Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76138.t1 | XP_013461367.1 | 87.8 | 49 | 6 | 0 | 1 | 49 | 1 | 49 | 4.80E-16 | 92.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76138.t1 | Q6R0C4 | 72.2 | 36 | 10 | 0 | 14 | 49 | 3 | 38 | 9.8e-10 | 63.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76138.t1 | A0A072V0T7 | 87.8 | 49 | 6 | 0 | 1 | 49 | 1 | 49 | 3.5e-16 | 92.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene76138.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76138.t1 | MTR_3g089045 | 87.755 | 49 | 6 | 0 | 1 | 49 | 1 | 49 | 5.13e-24 | 90.5 |
| MS.gene76138.t1 | MTR_4g082040 | 69.231 | 39 | 12 | 0 | 11 | 49 | 100 | 138 | 6.13e-13 | 60.8 |
| MS.gene76138.t1 | MTR_7g086960 | 64.103 | 39 | 14 | 0 | 11 | 49 | 84 | 122 | 9.24e-13 | 60.5 |
| MS.gene76138.t1 | MTR_6g074860 | 69.444 | 36 | 11 | 0 | 14 | 49 | 2 | 37 | 1.15e-12 | 59.3 |
| MS.gene76138.t1 | MTR_5g041570 | 66.667 | 39 | 13 | 0 | 11 | 49 | 129 | 167 | 1.32e-12 | 60.1 |
| MS.gene76138.t1 | MTR_6g027370 | 48.077 | 52 | 24 | 1 | 1 | 49 | 81 | 132 | 4.08e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76138.t1 | AT1G73410 | 75.000 | 36 | 9 | 0 | 14 | 49 | 4 | 39 | 6.34e-14 | 62.4 |
| MS.gene76138.t1 | AT1G73410 | 75.000 | 36 | 9 | 0 | 14 | 49 | 4 | 39 | 1.62e-13 | 62.0 |
| MS.gene76138.t1 | AT1G17950 | 72.222 | 36 | 10 | 0 | 14 | 49 | 3 | 38 | 2.00e-13 | 61.6 |
| MS.gene76138.t1 | AT1G69560 | 72.973 | 37 | 10 | 0 | 13 | 49 | 104 | 140 | 2.50e-12 | 58.9 |
| MS.gene76138.t1 | AT1G69560 | 72.973 | 37 | 10 | 0 | 13 | 49 | 120 | 156 | 2.56e-12 | 58.9 |
| MS.gene76138.t1 | AT4G33450 | 67.568 | 37 | 12 | 0 | 13 | 49 | 16 | 52 | 3.35e-12 | 58.2 |
| MS.gene76138.t1 | AT1G26780 | 78.788 | 33 | 7 | 0 | 17 | 49 | 99 | 131 | 4.84e-12 | 58.2 |
| MS.gene76138.t1 | AT1G26780 | 78.788 | 33 | 7 | 0 | 17 | 49 | 99 | 131 | 7.60e-12 | 57.8 |
| MS.gene76138.t1 | AT5G17800 | 60.000 | 40 | 16 | 0 | 10 | 49 | 87 | 126 | 2.01e-11 | 56.6 |
Find 0 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAATATGGTGCTCAAAAT+TGG | + | 23439:18822-18841 | MS.gene76138:CDS | 30.0% | |
| TCAATTGCTGAAAAACTTCA+AGG | + | 23439:18848-18867 | MS.gene76138:CDS | 30.0% | |
| CCATATTGTTCAACAAGTTG+AGG | - | 23439:18812-18831 | MS.gene76138:intergenic | 35.0% | |
| !! | CCTCAACTTGTTGAACAATA+TGG | + | 23439:18809-18828 | MS.gene76138:CDS | 35.0% |
| ! | GGAAGTTTTTCATCTTCAGC+AGG | - | 23439:18791-18810 | MS.gene76138:intergenic | 40.0% |
| GATACCAAGAGTTGTCCATG+TGG | + | 23439:18755-18774 | MS.gene76138:CDS | 45.0% | |
| AGAGTTGTCCATGTGGTCAC+TGG | + | 23439:18762-18781 | MS.gene76138:CDS | 50.0% | |
| GTGACCACATGGACAACTCT+TGG | - | 23439:18762-18781 | MS.gene76138:intergenic | 50.0% | |
| GCAGGTCGCCAGTGACCACA+TGG | - | 23439:18773-18792 | MS.gene76138:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 23439 | gene | 18728 | 18878 | 18728 | ID=MS.gene76138 |
| 23439 | mRNA | 18728 | 18878 | 18728 | ID=MS.gene76138.t1;Parent=MS.gene76138 |
| 23439 | exon | 18728 | 18878 | 18728 | ID=MS.gene76138.t1.exon1;Parent=MS.gene76138.t1 |
| 23439 | CDS | 18728 | 18878 | 18728 | ID=cds.MS.gene76138.t1;Parent=MS.gene76138.t1 |
| Gene Sequence |
| Protein sequence |
