AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76144

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76144.t1 MTR_2g099990 95.096 469 17 4 20 483 1 468 0.0 908
MS.gene76144.t1 MTR_2g099990 94.750 400 16 3 88 483 1 399 0.0 776
MS.gene76144.t1 MTR_8g017210 58.333 528 131 19 1 465 1 502 1.01e-176 506
MS.gene76144.t1 MTR_1g016010 49.091 495 181 15 12 480 3 452 1.32e-133 394
MS.gene76144.t1 MTR_1g094115 88.415 164 19 0 61 224 44 207 2.80e-110 337
MS.gene76144.t1 MTR_4g059870 42.589 479 191 15 61 475 35 493 3.25e-109 333
MS.gene76144.t1 MTR_1g112270 88.125 160 19 0 67 226 47 206 1.91e-107 331
MS.gene76144.t1 MTR_1g112270 84.337 166 26 0 61 226 52 217 3.18e-104 323
MS.gene76144.t1 MTR_2g093960 82.424 165 28 1 63 226 31 195 3.72e-102 312
MS.gene76144.t1 MTR_2g090745 87.582 153 19 0 74 226 17 169 4.53e-101 310
MS.gene76144.t1 MTR_1g094115 78.049 164 19 1 61 224 44 190 3.85e-93 292
MS.gene76144.t1 MTR_2g017720 60.606 165 51 2 33 188 273 432 1.24e-59 202
MS.gene76144.t1 MTR_3g102000 70.707 99 22 2 91 185 36 131 2.38e-41 147
MS.gene76144.t1 MTR_5g030560 76.000 50 12 0 69 118 11 60 1.35e-21 89.0
MS.gene76144.t1 MTR_1g093095 35.172 145 79 6 85 216 107 249 2.23e-18 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76144.t1 AT1G55110 49.895 477 169 15 16 470 17 445 2.43e-129 383
MS.gene76144.t1 AT1G55110 49.895 477 169 15 16 470 17 445 2.43e-129 383
MS.gene76144.t1 AT1G55110 49.895 477 169 15 16 470 17 445 2.43e-129 383
MS.gene76144.t1 AT3G13810 76.233 223 40 3 17 229 12 231 1.02e-117 355
MS.gene76144.t1 AT3G13810 36.782 174 86 8 315 482 353 508 5.09e-17 84.0
MS.gene76144.t1 AT3G13810 76.233 223 40 3 17 229 17 236 1.07e-117 355
MS.gene76144.t1 AT3G13810 36.782 174 86 8 315 482 358 513 6.29e-17 84.0
MS.gene76144.t1 AT3G13810 76.233 223 40 3 17 229 17 236 1.07e-117 355
MS.gene76144.t1 AT3G13810 36.782 174 86 8 315 482 358 513 6.29e-17 84.0
MS.gene76144.t1 AT3G13810 76.364 220 39 3 20 229 1 217 4.59e-116 350
MS.gene76144.t1 AT3G13810 36.782 174 86 8 315 482 339 494 4.57e-17 84.0
MS.gene76144.t1 AT3G50700 83.333 174 29 0 63 236 33 206 2.50e-112 339
MS.gene76144.t1 AT5G66730 73.077 208 39 1 19 226 4 194 3.14e-112 341
MS.gene76144.t1 AT1G03840 88.415 164 19 0 63 226 40 203 5.91e-112 340
MS.gene76144.t1 AT5G44160 82.222 180 27 1 52 226 20 199 1.36e-109 333
MS.gene76144.t1 AT1G14580 67.757 214 64 1 13 226 7 215 2.34e-108 330
MS.gene76144.t1 AT1G14580 67.757 214 64 1 13 226 7 215 2.34e-108 330
MS.gene76144.t1 AT4G02670 70.667 225 55 4 29 243 19 242 2.36e-108 328
MS.gene76144.t1 AT4G02670 70.667 225 55 4 29 243 17 240 2.39e-108 327
MS.gene76144.t1 AT1G14580 67.757 214 64 1 13 226 21 229 3.54e-108 330
MS.gene76144.t1 AT1G03840 87.195 164 19 1 63 226 40 201 4.67e-108 330
MS.gene76144.t1 AT2G02080 83.030 165 28 0 62 226 52 216 5.02e-108 330
MS.gene76144.t1 AT2G02080 83.030 165 28 0 62 226 52 216 5.02e-108 330
MS.gene76144.t1 AT2G02080 83.030 165 28 0 62 226 52 216 5.02e-108 330
MS.gene76144.t1 AT2G02080 83.030 165 28 0 62 226 52 216 5.02e-108 330
MS.gene76144.t1 AT3G45260 82.486 177 28 2 63 236 38 214 1.06e-107 327
MS.gene76144.t1 AT3G45260 82.486 177 28 2 63 236 38 214 1.06e-107 327
MS.gene76144.t1 AT5G60470 76.316 190 42 3 50 237 27 215 2.86e-106 324
MS.gene76144.t1 AT5G60470 76.316 190 42 3 50 237 31 219 2.96e-106 324
MS.gene76144.t1 AT3G13810 69.869 229 50 3 17 229 12 237 3.16e-106 326
MS.gene76144.t1 AT3G13810 36.782 174 86 8 315 482 359 514 6.54e-17 83.6
MS.gene76144.t1 AT2G02070 84.663 163 25 0 64 226 52 214 7.02e-105 325
MS.gene76144.t1 AT2G02070 84.663 163 25 0 64 226 52 214 7.02e-105 325
MS.gene76144.t1 AT3G13810 69.912 226 49 3 20 229 1 223 1.40e-104 321
MS.gene76144.t1 AT3G13810 36.782 174 86 8 315 482 345 500 6.26e-17 83.6
MS.gene76144.t1 AT5G03150 84.242 165 25 1 63 226 52 216 2.78e-103 318
MS.gene76144.t1 AT5G44160 86.928 153 20 0 74 226 8 160 1.02e-100 309
MS.gene76144.t1 AT5G60470 79.394 165 32 2 75 237 46 210 5.99e-97 300
MS.gene76144.t1 AT2G02080 83.453 139 23 0 88 226 1 139 3.37e-91 285
MS.gene76144.t1 AT2G02080 83.453 139 23 0 88 226 1 139 3.37e-91 285
MS.gene76144.t1 AT1G68130 64.103 156 54 2 64 217 41 196 1.39e-72 236
MS.gene76144.t1 AT2G01940 63.924 158 55 2 64 219 44 201 5.94e-72 235
MS.gene76144.t1 AT1G25250 59.538 173 59 3 64 233 33 197 1.29e-71 232
MS.gene76144.t1 AT2G01940 62.893 159 56 3 64 219 44 202 2.18e-68 226
MS.gene76144.t1 AT1G25250 60.736 163 53 3 74 233 20 174 3.06e-68 223
MS.gene76144.t1 AT1G68130 64.545 110 37 2 110 217 1 110 6.71e-46 163
MS.gene76144.t1 AT2G01940 63.393 112 39 2 110 219 1 112 2.00e-45 162
MS.gene76144.t1 AT1G25250 58.268 127 42 3 110 233 1 119 4.01e-45 160
MS.gene76144.t1 AT1G34370 30.841 214 125 6 59 254 3 211 8.33e-24 101
MS.gene76144.t1 AT1G34370 30.415 217 128 6 56 254 210 421 1.55e-23 103
MS.gene76144.t1 AT1G34370 30.415 217 128 6 56 254 210 421 1.55e-23 103
MS.gene76144.t1 AT5G22890 32.026 153 90 3 78 216 202 354 1.79e-21 96.3
MS.gene76144.t1 AT1G13290 34.161 161 91 7 76 224 85 242 3.92e-19 88.2
MS.gene76144.t1 AT3G20880 33.562 146 87 4 80 216 243 387 2.42e-18 87.4
MS.gene76144.t1 AT1G51220 32.192 146 89 4 80 216 165 309 6.54e-18 85.1
MS.gene76144.t1 AT3G57670 32.653 147 87 5 80 216 204 348 1.02e-17 85.1
MS.gene76144.t1 AT1G08290 36.765 136 73 6 92 216 179 312 7.39e-17 82.0
MS.gene76144.t1 AT1G34790 32.450 151 90 5 76 216 128 276 3.75e-16 79.7

Find 103 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTTCTTCTGGATTTCA+TGG 0.140432 2.3:-3792678 MS.gene76144:CDS
TTCTCTTGTGCCACAGGTTT+TGG 0.189840 2.3:+3793804 None:intergenic
AACAACTCTCTCTCTTCTTC+TGG 0.241809 2.3:-3792687 MS.gene76144:CDS
GGTATTTGTACTTCTTGTTC+TGG 0.297642 2.3:+3793035 None:intergenic
GAAGCTAGTGCTAATTCTTC+TGG 0.298581 2.3:-3794726 MS.gene76144:CDS
GGAAGTGTAGGACCATTTCT+AGG 0.313383 2.3:+3792927 None:intergenic
AGCCTTGGATATCTTGAGTT+TGG 0.314047 2.3:+3793099 None:intergenic
TTTGTTGCCATCAGTGTCTT+TGG 0.324056 2.3:+3794117 None:intergenic
GCACTTACAAGAGATTTCTT+AGG 0.351186 2.3:-3792519 MS.gene76144:CDS
AGAGAGAGTTGTTTATCATT+TGG 0.355557 2.3:+3792697 None:intergenic
ACCTCTCCTCAAACTATTCA+AGG 0.372455 2.3:-3792986 MS.gene76144:CDS
GTAGGACCATTTCTAGGGTT+AGG 0.377918 2.3:+3792933 None:intergenic
ATCAAAGTTTCAACATGTTA+AGG 0.397845 2.3:-3793058 MS.gene76144:CDS
ATCCAACCTTGAATAGTTTG+AGG 0.405976 2.3:+3792980 None:intergenic
TTGTGTAGGTTGTGGAATTA+CGG 0.407829 2.3:+3793151 None:intergenic
AGTGATATCCTCACCATTGC+TGG 0.409995 2.3:-3792477 MS.gene76144:CDS
TCTAATTTGACTTCTGCATC+TGG 0.412009 2.3:-3794750 MS.gene76144:CDS
TAGGACCATTTCTAGGGTTA+GGG 0.414611 2.3:+3792934 None:intergenic
TGGTGCACACAAGTTGGTTC+TGG 0.417382 2.3:+3793958 None:intergenic
TATCCTCACCATTGCTGGTA+TGG 0.421802 2.3:-3792472 MS.gene76144:CDS
CATGACCCATCAAGAGCCTT+GGG 0.426349 2.3:-3793937 MS.gene76144:CDS
GTTGAGTGGGACCCACCAAC+AGG 0.427753 2.3:-3792805 MS.gene76144:CDS
GTTCTTTGCTTCAACTTCCA+TGG 0.431659 2.3:+3794015 None:intergenic
TCTGTATTCTCTTGTGCCAC+AGG 0.435175 2.3:+3793798 None:intergenic
AAGAAAAGAAGAAACCTTCC+TGG 0.442018 2.3:-3794621 MS.gene76144:CDS
ATTTGAGTTTATCATGTTGT+TGG 0.445102 2.3:+3792755 None:intergenic
GTTTGAGGAGAGGTCAATGT+TGG 0.446017 2.3:+3792995 None:intergenic
CTTTCTTCAATGTCAAGCCT+TGG 0.446505 2.3:+3793084 None:intergenic
AAGCTATAACTTCAGCTTGT+GGG 0.447498 2.3:+3794145 None:intergenic
GAAAACCCTAACCCTAGAAA+TGG 0.454115 2.3:-3792939 MS.gene76144:CDS
AGTGCTTCTTGATACCAGTT+AGG 0.454887 2.3:+3793911 None:intergenic
GAAAATGAACCTGGATTGCC+AGG 0.461039 2.3:+3794603 None:intergenic
CAGAGCCTTCTGTGATGCAT+TGG 0.469401 2.3:-3793192 MS.gene76144:CDS
TGCATTGTTTCCTCCGGCAC+CGG 0.471541 2.3:+3792539 None:intergenic
AGAAACCTTCCTGGCAATCC+AGG 0.472577 2.3:-3794612 MS.gene76144:intron
CCAAGGCTCTTGATGGGTCA+TGG 0.475409 2.3:+3793938 None:intergenic
TGTAAGTGCATTGTTTCCTC+CGG 0.475628 2.3:+3792533 None:intergenic
CCATGACCCATCAAGAGCCT+TGG 0.490603 2.3:-3793938 MS.gene76144:CDS
ACCGGCACCGTCATCATCAT+TGG 0.490777 2.3:+3792557 None:intergenic
AACAACATGATAAACTCAAA+TGG 0.493838 2.3:-3792753 MS.gene76144:CDS
AATCTTCAACTTCATAAAAG+AGG 0.500458 2.3:-3794048 MS.gene76144:CDS
ATCCTCACCATTGCTGGTAT+GGG 0.502220 2.3:-3792471 MS.gene76144:CDS
AGTTGATGCAAAATTCTCTC+AGG 0.503692 2.3:-3794691 MS.gene76144:CDS
GCTTGATGATCGGAGTTGGA+AGG 0.505394 2.3:+3792435 None:intergenic
GATGATGACGGTGCCGGTGC+CGG 0.516820 2.3:-3792552 MS.gene76144:CDS
GTTACAGAAAGCAGCTCAGA+TGG 0.517581 2.3:-3792853 MS.gene76144:CDS
CTTGATGATCGGAGTTGGAA+GGG 0.517775 2.3:+3792436 None:intergenic
ATGAACCTGGATTGCCAGGA+AGG 0.520133 2.3:+3794607 None:intergenic
ATAGTTATACCTTGAGAAGA+GGG 0.521705 2.3:+3793762 None:intergenic
ATATGTGAGATCTGTAACAA+AGG 0.526520 2.3:-3794087 MS.gene76144:CDS
AAGAAGTACAAATACCCTCT+TGG 0.528888 2.3:-3793028 MS.gene76144:CDS
ACAACAATCATTAGTTGTTG+AGG 0.530940 2.3:-3794781 MS.gene76144:CDS
AGTATGCAGTTCAATCAGAC+TGG 0.532991 2.3:-3793837 MS.gene76144:CDS
TTTGGAGATTGTTCATGTTG+TGG 0.533091 2.3:+3793117 None:intergenic
AAAGAACAAGCAATGAGATA+AGG 0.533182 2.3:-3793999 MS.gene76144:CDS
CTCCTCAAACTATTCAAGGT+TGG 0.534048 2.3:-3792982 MS.gene76144:CDS
TGAGGTGAAGCTGCAGATGC+TGG 0.537360 2.3:+3792894 None:intergenic
TGAGTTTATCATGTTGTTGG+TGG 0.541113 2.3:+3792758 None:intergenic
GCAGATGCTGGTTGGTAGGA+TGG 0.542786 2.3:+3792906 None:intergenic
TTGGTGGGTCCCACTCAACA+TGG 0.546559 2.3:+3792809 None:intergenic
CCAAACTAATTACCTTCCCA+TGG 0.548476 2.3:+3792396 None:intergenic
GTTTGCTTGATGATCGGAGT+TGG 0.548511 2.3:+3792431 None:intergenic
CAACATGGACTGAGATGAGC+TGG 0.548999 2.3:+3792824 None:intergenic
TATCATTTGGTCTTCACATG+AGG 0.559455 2.3:+3792710 None:intergenic
GGAATGGTTTGCTTGATGAT+CGG 0.561157 2.3:+3792425 None:intergenic
TATAGTTATACCTTGAGAAG+AGG 0.562344 2.3:+3793761 None:intergenic
GAACATAATATCAATCATGA+TGG 0.565329 2.3:-3792606 MS.gene76144:CDS
GAAAGCACGGTGAGAAGAAG+TGG 0.566601 2.3:-3793882 MS.gene76144:CDS
GAAGTGTAGGACCATTTCTA+GGG 0.573649 2.3:+3792928 None:intergenic
ACCTTGAATAGTTTGAGGAG+AGG 0.575888 2.3:+3792985 None:intergenic
TCATCTCAGTCCATGTTGAG+TGG 0.578988 2.3:-3792819 MS.gene76144:CDS
CTCAGCCAATGCATCACAGA+AGG 0.582209 2.3:+3793187 None:intergenic
TAGACACGTGACCCTGTTGG+TGG 0.583962 2.3:+3792793 None:intergenic
CTTCTGTGATGCATTGGCTG+AGG 0.585782 2.3:-3793186 MS.gene76144:CDS
GGCACCGTCATCATCATTGG+TGG 0.585980 2.3:+3792560 None:intergenic
AAAGAGGACACAATTTGCCA+TGG 0.587343 2.3:-3794032 MS.gene76144:CDS
CATCTCAGTCCATGTTGAGT+GGG 0.587878 2.3:-3792818 MS.gene76144:CDS
CTCCAAACTCAAGATATCCA+AGG 0.596098 2.3:-3793101 MS.gene76144:CDS
GCAACTTCCCATACCAGCAA+TGG 0.602937 2.3:+3792464 None:intergenic
AAAGCTATAACTTCAGCTTG+TGG 0.603171 2.3:+3794144 None:intergenic
AAGCACTTCTGCAGAAAGCA+CGG 0.603251 2.3:-3793895 MS.gene76144:CDS
TTGGATTGACATAGCCAAGA+GGG 0.612593 2.3:+3793014 None:intergenic
GATGACGGTGCCGGTGCCGG+AGG 0.615538 2.3:-3792549 MS.gene76144:CDS
TTCCCATACCAGCAATGGTG+AGG 0.621943 2.3:+3792469 None:intergenic
CAATAGACACGTGACCCTGT+TGG 0.624582 2.3:+3792790 None:intergenic
ACCAATGATGATGACGGTGC+CGG 0.625084 2.3:-3792558 MS.gene76144:CDS
ACTGTGGAACCCTCTTCTCA+AGG 0.626939 2.3:-3793771 MS.gene76144:intron
AGCTGCAGATGCTGGTTGGT+AGG 0.631564 2.3:+3792902 None:intergenic
TCAACCACCAATGATGATGA+CGG 0.633146 2.3:-3792564 MS.gene76144:CDS
GGTTGGTAGGATGGAAGTGT+AGG 0.641310 2.3:+3792915 None:intergenic
TTACAGAAAGCAGCTCAGAT+GGG 0.645481 2.3:-3792852 MS.gene76144:CDS
GTGAAGCTGCAGATGCTGGT+TGG 0.647374 2.3:+3792898 None:intergenic
AGACACGTGACCCTGTTGGT+GGG 0.651658 2.3:+3792794 None:intergenic
TGACCCATCAAGAGCCTTGG+GGG 0.652313 2.3:-3793935 MS.gene76144:CDS
TTTGTCACCAAAGACACTGA+TGG 0.656547 2.3:-3794124 MS.gene76144:CDS
AGGATATCACTATCAGAGAG+TGG 0.656639 2.3:+3792489 None:intergenic
GGGTCATGGTGCACACAAGT+TGG 0.674016 2.3:+3793952 None:intergenic
TTGAGTGGGACCCACCAACA+GGG 0.675919 2.3:-3792804 MS.gene76144:CDS
AAAGCTCACTCCAAAACCTG+TGG 0.698299 2.3:-3793814 MS.gene76144:CDS
AGAGAATACAGATGTGACTG+TGG 0.700052 2.3:-3793787 MS.gene76144:CDS
ATGACCCATCAAGAGCCTTG+GGG 0.705499 2.3:-3793936 MS.gene76144:CDS
GTTGGATTGACATAGCCAAG+AGG 0.725600 2.3:+3793013 None:intergenic
AATGCTGTAGCTGACATGTG+AGG 0.760209 2.3:+3792876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATATTATATGATTTTC+AGG - chr2.3:3793976-3793995 MS.gene76144:CDS 10.0%
!!! TTTTATTAATAACTTAGTTT+AGG + chr2.3:3793502-3793521 None:intergenic 10.0%
!! ATCTTAATTGTTATAAATTG+TGG - chr2.3:3792656-3792675 MS.gene76144:CDS 15.0%
!!! CATATTTTGTTTATCTAAAT+TGG - chr2.3:3792995-3793014 MS.gene76144:CDS 15.0%
!! ATTAAGATGAAAAGAAAAGA+TGG + chr2.3:3792644-3792663 None:intergenic 20.0%
!! GAAAAATATGAAGAAAAACA+TGG - chr2.3:3792722-3792741 MS.gene76144:CDS 20.0%
!! TATACTATTATCAACTTGAA+GGG + chr2.3:3793544-3793563 None:intergenic 20.0%
!!! AATATTATATGATTTTCAGG+AGG - chr2.3:3793979-3793998 MS.gene76144:CDS 20.0%
!!! ATATTATATGATTTTCAGGA+GGG - chr2.3:3793980-3793999 MS.gene76144:CDS 20.0%
!!! CAAACATTTTTTAAAGAAAG+TGG - chr2.3:3793612-3793631 MS.gene76144:intron 20.0%
!!! GAAAGTGGAAATTTTTAAAA+TGG - chr2.3:3793627-3793646 MS.gene76144:intron 20.0%
!!! TTTTTAATGTACTATCTCTT+TGG - chr2.3:3793662-3793681 MS.gene76144:intron 20.0%
! AACAACATGATAAACTCAAA+TGG - chr2.3:3794458-3794477 MS.gene76144:intron 25.0%
! AATCTTCAACTTCATAAAAG+AGG - chr2.3:3793163-3793182 MS.gene76144:CDS 25.0%
! ATCAAAGTTTCAACATGTTA+AGG - chr2.3:3794153-3794172 MS.gene76144:intron 25.0%
! GAACATAATATCAATCATGA+TGG - chr2.3:3794605-3794624 MS.gene76144:intron 25.0%
! GTATACTATTATCAACTTGA+AGG + chr2.3:3793545-3793564 None:intergenic 25.0%
! TTTAACATGTTATGCTTGTT+TGG - chr2.3:3792843-3792862 MS.gene76144:CDS 25.0%
!! ATTTGAGTTTATCATGTTGT+TGG + chr2.3:3794459-3794478 None:intergenic 25.0%
!! CATATTTTTCCACAATATCA+TGG + chr2.3:3792712-3792731 None:intergenic 25.0%
AAAAATACAAAAGTGAGGTG+AGG + chr2.3:3793946-3793965 None:intergenic 30.0%
AAAGAACAAGCAATGAGATA+AGG - chr2.3:3793212-3793231 MS.gene76144:CDS 30.0%
ACAACAATCATTAGTTGTTG+AGG - chr2.3:3792430-3792449 MS.gene76144:CDS 30.0%
ACTTAGTATTCTCAAGTCTA+AGG - chr2.3:3793837-3793856 MS.gene76144:CDS 30.0%
ACTTGAAGGGATAAAATATG+TGG + chr2.3:3793531-3793550 None:intergenic 30.0%
AGAACTTAAGAAAATGAACC+TGG + chr2.3:3792620-3792639 None:intergenic 30.0%
AGAGAGAGTTGTTTATCATT+TGG + chr2.3:3794517-3794536 None:intergenic 30.0%
ATAAATTGTGGTTATAGTCG+CGG - chr2.3:3792668-3792687 MS.gene76144:CDS 30.0%
ATAGTTATACCTTGAGAAGA+GGG + chr2.3:3793452-3793471 None:intergenic 30.0%
ATATGTGAGATCTGTAACAA+AGG - chr2.3:3793124-3793143 MS.gene76144:CDS 30.0%
CAACAAAATCATACTAACAC+CGG - chr2.3:3793789-3793808 MS.gene76144:CDS 30.0%
CACACAAAAATACAAAAGTG+AGG + chr2.3:3793951-3793970 None:intergenic 30.0%
GAATATTTCTTGGCTGTTAT+AGG - chr2.3:3793906-3793925 MS.gene76144:CDS 30.0%
GCAATGACATCTAATTAAAC+CGG - chr2.3:3792779-3792798 MS.gene76144:CDS 30.0%
TATAGTTATACCTTGAGAAG+AGG + chr2.3:3793453-3793472 None:intergenic 30.0%
TGTACTATCTCTTTGGTTAT+CGG - chr2.3:3793669-3793688 MS.gene76144:intron 30.0%
TTAATTAGATGTCATTGCAG+TGG + chr2.3:3792777-3792796 None:intergenic 30.0%
! ATTTCATGGAACTTCTTTTG+AGG - chr2.3:3794547-3794566 MS.gene76144:intron 30.0%
! TGGGATAAATGTGAATTTTC+CGG + chr2.3:3793811-3793830 None:intergenic 30.0%
AAACCATTCCCAAAAACCAT+GGG - chr2.3:3794799-3794818 MS.gene76144:CDS 35.0%
AAAGCTATAACTTCAGCTTG+TGG + chr2.3:3793070-3793089 None:intergenic 35.0%
AAGAAAAGAAGAAACCTTCC+TGG - chr2.3:3792590-3792609 MS.gene76144:CDS 35.0%
AAGCTATAACTTCAGCTTGT+GGG + chr2.3:3793069-3793088 None:intergenic 35.0%
ATAGTGCATGCTTAGATTGA+TGG - chr2.3:3793695-3793714 MS.gene76144:intron 35.0%
ATCCAACCTTGAATAGTTTG+AGG + chr2.3:3794234-3794253 None:intergenic 35.0%
GAGAATACTAAGTGCATGTT+GGG + chr2.3:3793830-3793849 None:intergenic 35.0%
GCACTTACAAGAGATTTCTT+AGG - chr2.3:3794692-3794711 MS.gene76144:CDS 35.0%
GGTATTTGTACTTCTTGTTC+TGG + chr2.3:3794179-3794198 None:intergenic 35.0%
GTGAAACAGAAAATGAGAGA+GGG - chr2.3:3793882-3793901 MS.gene76144:CDS 35.0%
TGAGAATACTAAGTGCATGT+TGG + chr2.3:3793831-3793850 None:intergenic 35.0%
TGAGTTTATCATGTTGTTGG+TGG + chr2.3:3794456-3794475 None:intergenic 35.0%
TGTGAAACAGAAAATGAGAG+AGG - chr2.3:3793881-3793900 MS.gene76144:CDS 35.0%
TTGTGTAGGTTGTGGAATTA+CGG + chr2.3:3794063-3794082 None:intergenic 35.0%
TTTGGAGATTGTTCATGTTG+TGG + chr2.3:3794097-3794116 None:intergenic 35.0%
! AAGAAGTACAAATACCCTCT+TGG - chr2.3:3794183-3794202 MS.gene76144:intron 35.0%
! AATTACCTTCCCATGGTTTT+TGG + chr2.3:3794811-3794830 None:intergenic 35.0%
! AGGTTTCTTCTTTTCTTAGC+TGG + chr2.3:3792587-3792606 None:intergenic 35.0%
! AGTTGATGCAAAATTCTCTC+AGG - chr2.3:3792520-3792539 MS.gene76144:CDS 35.0%
! ATTACCTTCCCATGGTTTTT+GGG + chr2.3:3794810-3794829 None:intergenic 35.0%
! GAAGAAAAACATGGTTGATG+CGG - chr2.3:3792731-3792750 MS.gene76144:CDS 35.0%
! GTTATGCTTGTTTGGAATCA+TGG - chr2.3:3792851-3792870 MS.gene76144:CDS 35.0%
! TAGGGTTAGGGTTTTCATAA+AGG + chr2.3:3794268-3794287 None:intergenic 35.0%
! TATCATTTGGTCTTCACATG+AGG + chr2.3:3794504-3794523 None:intergenic 35.0%
! TCTAATTTGACTTCTGCATC+TGG - chr2.3:3792461-3792480 MS.gene76144:CDS 35.0%
!! GAATTTTGTTGTGTAGGTTG+TGG + chr2.3:3794071-3794090 None:intergenic 35.0%
!! TCTTTTGAGGATACTTTTGC+TGG - chr2.3:3794560-3794579 MS.gene76144:intron 35.0%
!! TGGTGAGAATTTTGTTGTGT+AGG + chr2.3:3794077-3794096 None:intergenic 35.0%
AAAGAGGACACAATTTGCCA+TGG - chr2.3:3793179-3793198 MS.gene76144:CDS 40.0%
AACAACTCTCTCTCTTCTTC+TGG - chr2.3:3794524-3794543 MS.gene76144:intron 40.0%
AATATGAATGCGCGCATGTT+TGG + chr2.3:3792981-3793000 None:intergenic 40.0%
ACCTCTCCTCAAACTATTCA+AGG - chr2.3:3794225-3794244 MS.gene76144:intron 40.0%
ACCTTGAATAGTTTGAGGAG+AGG + chr2.3:3794229-3794248 None:intergenic 40.0%
AGAGAATACAGATGTGACTG+TGG - chr2.3:3793424-3793443 MS.gene76144:intron 40.0%
AGCCTTGGATATCTTGAGTT+TGG + chr2.3:3794115-3794134 None:intergenic 40.0%
AGGATATCACTATCAGAGAG+TGG + chr2.3:3794725-3794744 None:intergenic 40.0%
AGTATGCAGTTCAATCAGAC+TGG - chr2.3:3793374-3793393 MS.gene76144:intron 40.0%
AGTGCTTCTTGATACCAGTT+AGG + chr2.3:3793303-3793322 None:intergenic 40.0%
CAAACCATTCCCAAAAACCA+TGG - chr2.3:3794798-3794817 MS.gene76144:CDS 40.0%
CGCTATGAACCATGATATTG+TGG - chr2.3:3792700-3792719 MS.gene76144:CDS 40.0%
CTCCAAACTCAAGATATCCA+AGG - chr2.3:3794110-3794129 MS.gene76144:CDS 40.0%
CTCCTCAAACTATTCAAGGT+TGG - chr2.3:3794229-3794248 MS.gene76144:intron 40.0%
CTCGAGCTTCAACAAAATCA+CGG + chr2.3:3792899-3792918 None:intergenic 40.0%
CTCTCTTCTTCTGGATTTCA+TGG - chr2.3:3794533-3794552 MS.gene76144:intron 40.0%
CTTTCTTCAATGTCAAGCCT+TGG + chr2.3:3794130-3794149 None:intergenic 40.0%
GAAAACCCTAACCCTAGAAA+TGG - chr2.3:3794272-3794291 MS.gene76144:intron 40.0%
GAAGCTAGTGCTAATTCTTC+TGG - chr2.3:3792485-3792504 MS.gene76144:CDS 40.0%
GAAGTGTAGGACCATTTCTA+GGG + chr2.3:3794286-3794305 None:intergenic 40.0%
GAGAGAGGGTGAATATTTCT+TGG - chr2.3:3793896-3793915 MS.gene76144:CDS 40.0%
GTTCTTTGCTTCAACTTCCA+TGG + chr2.3:3793199-3793218 None:intergenic 40.0%
TGGCATCACAACTACAATTG+CGG + chr2.3:3792757-3792776 None:intergenic 40.0%
TGTAAGTGCATTGTTTCCTC+CGG + chr2.3:3794681-3794700 None:intergenic 40.0%
TTACAGAAAGCAGCTCAGAT+GGG - chr2.3:3794359-3794378 MS.gene76144:intron 40.0%
TTGGATTGACATAGCCAAGA+GGG + chr2.3:3794200-3794219 None:intergenic 40.0%
TTTGTCACCAAAGACACTGA+TGG - chr2.3:3793087-3793106 MS.gene76144:CDS 40.0%
TTTGTTGCCATCAGTGTCTT+TGG + chr2.3:3793097-3793116 None:intergenic 40.0%
! TCAACCACCAATGATGATGA+CGG - chr2.3:3794647-3794666 MS.gene76144:CDS 40.0%
!! GGAATGGTTTGCTTGATGAT+CGG + chr2.3:3794789-3794808 None:intergenic 40.0%
!! TAGGACCATTTCTAGGGTTA+GGG + chr2.3:3794280-3794299 None:intergenic 40.0%
AAAGCTCACTCCAAAACCTG+TGG - chr2.3:3793397-3793416 MS.gene76144:intron 45.0%
AAGCACTTCTGCAGAAAGCA+CGG - chr2.3:3793316-3793335 MS.gene76144:intron 45.0%
AATGCTGTAGCTGACATGTG+AGG + chr2.3:3794338-3794357 None:intergenic 45.0%
AGTGATATCCTCACCATTGC+TGG - chr2.3:3794734-3794753 MS.gene76144:CDS 45.0%
ATCCTCACCATTGCTGGTAT+GGG - chr2.3:3794740-3794759 MS.gene76144:CDS 45.0%
CATCTCAGTCCATGTTGAGT+GGG - chr2.3:3794393-3794412 MS.gene76144:intron 45.0%
CATTCCCAAAAACCATGGGA+AGG - chr2.3:3794803-3794822 MS.gene76144:CDS 45.0%
CGTTTGCAGCAGCAATATAC+CGG + chr2.3:3792801-3792820 None:intergenic 45.0%
GAAAATGAACCTGGATTGCC+AGG + chr2.3:3792611-3792630 None:intergenic 45.0%
GAGCTTCAACAAAATCACGG+TGG + chr2.3:3792896-3792915 None:intergenic 45.0%
GTTACAGAAAGCAGCTCAGA+TGG - chr2.3:3794358-3794377 MS.gene76144:intron 45.0%
GTTGGATTGACATAGCCAAG+AGG + chr2.3:3794201-3794220 None:intergenic 45.0%
GTTTGAGGAGAGGTCAATGT+TGG + chr2.3:3794219-3794238 None:intergenic 45.0%
TATCCTCACCATTGCTGGTA+TGG - chr2.3:3794739-3794758 MS.gene76144:CDS 45.0%
TCATCTCAGTCCATGTTGAG+TGG - chr2.3:3794392-3794411 MS.gene76144:intron 45.0%
TCTGTATTCTCTTGTGCCAC+AGG + chr2.3:3793416-3793435 None:intergenic 45.0%
! CTTGATGATCGGAGTTGGAA+GGG + chr2.3:3794778-3794797 None:intergenic 45.0%
! GGAAGTGTAGGACCATTTCT+AGG + chr2.3:3794287-3794306 None:intergenic 45.0%
! GTTTGCTTGATGATCGGAGT+TGG + chr2.3:3794783-3794802 None:intergenic 45.0%
! TTCTCTTGTGCCACAGGTTT+TGG + chr2.3:3793410-3793429 None:intergenic 45.0%
!! CTTCCCATGGTTTTTGGGAA+TGG + chr2.3:3794805-3794824 None:intergenic 45.0%
!! GTAGGACCATTTCTAGGGTT+AGG + chr2.3:3794281-3794300 None:intergenic 45.0%
ACTGTGGAACCCTCTTCTCA+AGG - chr2.3:3793440-3793459 MS.gene76144:intron 50.0%
AGAAACCTTCCTGGCAATCC+AGG - chr2.3:3792599-3792618 MS.gene76144:CDS 50.0%
ATGAACCTGGATTGCCAGGA+AGG + chr2.3:3792607-3792626 None:intergenic 50.0%
ATGACCCATCAAGAGCCTTG+GGG - chr2.3:3793275-3793294 MS.gene76144:intron 50.0%
CAATAGACACGTGACCCTGT+TGG + chr2.3:3794424-3794443 None:intergenic 50.0%
CAGAGCCTTCTGTGATGCAT+TGG - chr2.3:3794019-3794038 MS.gene76144:CDS 50.0%
CATGACCCATCAAGAGCCTT+GGG - chr2.3:3793274-3793293 MS.gene76144:intron 50.0%
CTCAGCCAATGCATCACAGA+AGG + chr2.3:3794027-3794046 None:intergenic 50.0%
GCAACTTCCCATACCAGCAA+TGG + chr2.3:3794750-3794769 None:intergenic 50.0%
GCGTAGCTTCTGCAAACTCA+CGG - chr2.3:3792920-3792939 MS.gene76144:CDS 50.0%
TTCCCATACCAGCAATGGTG+AGG + chr2.3:3794745-3794764 None:intergenic 50.0%
! CAACATGGACTGAGATGAGC+TGG + chr2.3:3794390-3794409 None:intergenic 50.0%
! GAAAGCACGGTGAGAAGAAG+TGG - chr2.3:3793329-3793348 MS.gene76144:intron 50.0%
! GCTTGATGATCGGAGTTGGA+AGG + chr2.3:3794779-3794798 None:intergenic 50.0%
!! ACCAATGATGATGACGGTGC+CGG - chr2.3:3794653-3794672 MS.gene76144:CDS 50.0%
!! CTTCTGTGATGCATTGGCTG+AGG - chr2.3:3794025-3794044 MS.gene76144:CDS 50.0%
!! GGTTGGTAGGATGGAAGTGT+AGG + chr2.3:3794299-3794318 None:intergenic 50.0%
!! TGGTGCACACAAGTTGGTTC+TGG + chr2.3:3793256-3793275 None:intergenic 50.0%
ACCGGCACCGTCATCATCAT+TGG + chr2.3:3794657-3794676 None:intergenic 55.0%
AGACACGTGACCCTGTTGGT+GGG + chr2.3:3794420-3794439 None:intergenic 55.0%
CCATGACCCATCAAGAGCCT+TGG - chr2.3:3793273-3793292 MS.gene76144:intron 55.0%
GATACCAGTTAGGTCCCCCA+AGG + chr2.3:3793293-3793312 None:intergenic 55.0%
GGCACCGTCATCATCATTGG+TGG + chr2.3:3794654-3794673 None:intergenic 55.0%
GGGTCATGGTGCACACAAGT+TGG + chr2.3:3793262-3793281 None:intergenic 55.0%
GTGAAGCTGCAGATGCTGGT+TGG + chr2.3:3794316-3794335 None:intergenic 55.0%
TAGACACGTGACCCTGTTGG+TGG + chr2.3:3794421-3794440 None:intergenic 55.0%
TGACCCATCAAGAGCCTTGG+GGG - chr2.3:3793276-3793295 MS.gene76144:intron 55.0%
TGAGGTGAAGCTGCAGATGC+TGG + chr2.3:3794320-3794339 None:intergenic 55.0%
TGCATTGTTTCCTCCGGCAC+CGG + chr2.3:3794675-3794694 None:intergenic 55.0%
TTGAGTGGGACCCACCAACA+GGG - chr2.3:3794407-3794426 MS.gene76144:intron 55.0%
! AGCTGCAGATGCTGGTTGGT+AGG + chr2.3:3794312-3794331 None:intergenic 55.0%
! CCAAGGCTCTTGATGGGTCA+TGG + chr2.3:3793276-3793295 None:intergenic 55.0%
! TTGGTGGGTCCCACTCAACA+TGG + chr2.3:3794405-3794424 None:intergenic 55.0%
!! GCAGATGCTGGTTGGTAGGA+TGG + chr2.3:3794308-3794327 None:intergenic 55.0%
AGAGCCTTGGGGGACCTAAC+TGG - chr2.3:3793286-3793305 MS.gene76144:intron 60.0%
AGGTCCCCCAAGGCTCTTGA+TGG + chr2.3:3793283-3793302 None:intergenic 60.0%
GGTCCCCCAAGGCTCTTGAT+GGG + chr2.3:3793282-3793301 None:intergenic 60.0%
GTTGAGTGGGACCCACCAAC+AGG - chr2.3:3794406-3794425 MS.gene76144:intron 60.0%
!! GATGATGACGGTGCCGGTGC+CGG - chr2.3:3794659-3794678 MS.gene76144:CDS 65.0%
!! GATGACGGTGCCGGTGCCGG+AGG - chr2.3:3794662-3794681 MS.gene76144:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 3792401 3794832 3792401 ID=MS.gene76144
chr2.3 mRNA 3792401 3794832 3792401 ID=MS.gene76144.t1;Parent=MS.gene76144
chr2.3 exon 3794613 3794832 3794613 ID=MS.gene76144.t1.exon1;Parent=MS.gene76144.t1
chr2.3 CDS 3794613 3794832 3794613 ID=cds.MS.gene76144.t1;Parent=MS.gene76144.t1
chr2.3 exon 3793772 3794168 3793772 ID=MS.gene76144.t1.exon2;Parent=MS.gene76144.t1
chr2.3 CDS 3793772 3794168 3793772 ID=cds.MS.gene76144.t1;Parent=MS.gene76144.t1
chr2.3 exon 3792401 3793235 3792401 ID=MS.gene76144.t1.exon3;Parent=MS.gene76144.t1
chr2.3 CDS 3792401 3793235 3792401 ID=cds.MS.gene76144.t1;Parent=MS.gene76144.t1
Gene Sequence

>MS.gene76144

ATGATCAAAGGTTTTATGTCTCAACAAGAACAACAATCATTAGTTGTTGAGGAAAACATGTCTAATTTGACTTCTGCATCTGGTGAAGCTAGTGCTAATTCTTCTGGAAACAGAACTGAAGTTGATGCAAAATTCTCTCAGGAATACTTTGCTTCATCACAAACTCAAACTCATGATGAGACTCCAGCTAAGAAAAGAAGAAACCTTCCTGGCAATCCAGGTTCATTTTCTTAAGTTCTACCATCTTTTCTTTTCATCTTAATTGTTATAAATTGTGGTTATAGTCGCGGTTATGATTACGCTATGAACCATGATATTGTGGAAAAATATGAAGAAAAACATGGTTGATGCGGCCGCAATTGTAGTTGTGATGCCACTGCAATGACATCTAATTAAACCGGTATATTGCTGCTGCAAACGACAATTTAGAACTATATGTGTATTTAACATGTTATGCTTGTTTGGAATCATGGTATAGATTTGACAAAGTTACCACCGTGATTTTGTTGAAGCTCGAGAGCGTAGCTTCTGCAAACTCACGGTGACTGACAGTAATTTTATCAAACTCATCATGAATCCAAACATGCGCGCATTCATATTTTGTTTATCTAAATTGGATTTCTTTTGTTTTGTGTTTGTTTTTTTATTTGATTATTCATTTTAGACCCACAAGCTGAAGTTATAGCTTTGTCACCAAAGACACTGATGGCAACAAATAGATTCATATGTGAGATCTGTAACAAAGGATTTCAAAGAGATCAGAATCTTCAACTTCATAAAAGAGGACACAATTTGCCATGGAAGTTGAAGCAAAGAACAAGCAATGAGATAAGGAAGAAAGTGTATGTGTGTCCAGAACCAACTTGTGTGCACCATGACCCATCAAGAGCCTTGGGGGACCTAACTGGTATCAAGAAGCACTTCTGCAGAAAGCACGGTGAGAAGAAGTGGAAATGTGACAAGTGCTCTAAGAAGTATGCAGTTCAATCAGACTGGAAAGCTCACTCCAAAACCTGTGGCACAAGAGAATACAGATGTGACTGTGGAACCCTCTTCTCAAGGTATAACTATAACTATAACTATAAATATATTAACTAACCTAAACTAAGTTATTAATAAAATAATAACCACATATTTTATCCCTTCAAGTTGATAATAGTATACTGAACATGTAAAAAGAGTTCAATACATTGATCATTGATAGATAAAATCAAACATTTTTTAAAGAAAGTGGAAATTTTTAAAATGGAAAACTGATTTTTTTTTAATGTACTATCTCTTTGGTTATCGGTGCATAGTGCATGCTTAGATTGATGGTGAATTTCTCAGAATCATTTAGAATCACATTGTATCATTGTAATTTTGACAAAAGTTATACTTTAGAGTTTCAACAAAATCATACTAACACCGGAAAATTCACATTTATCCCAACATGCACTTAGTATTCTCAAGTCTAAGGAGAGTGAGACTAAAAAAATGATGTGAAACAGAAAATGAGAGAGGGTGAATATTTCTTGGCTGTTATAGGATTTTAGTAAAAAACCTCACCTCACTTTTGTATTTTTGTGTGTTTGTTTAAATATTATATGATTTTCAGGAGGGACAGTTTCATAACTCACAGAGCCTTCTGTGATGCATTGGCTGAGGAGAGTTCAAGAACCGTAATTCCACAACCTACACAACAAAATTCTCACCACAACATGAACAATCTCCAAACTCAAGATATCCAAGGCTTGACATTGAAGAAAGAGCATCAAAGTTTCAACATGTTAAGGCCAGAACAAGAAGTACAAATACCCTCTTGGCTATGTCAATCCAACATTGACCTCTCCTCAAACTATTCAAGGTTGGATCAAGATCTACACCTTTATGAAAACCCTAACCCTAGAAATGGTCCTACACTTCCATCCTACCAACCAGCATCTGCAGCTTCACCTCACATGTCAGCTACAGCATTGTTACAGAAAGCAGCTCAGATGGGTGCAACCAGCTCATCTCAGTCCATGTTGAGTGGGACCCACCAACAGGGTCACGTGTCTATTGTTGACTCTGCCACCAACAACATGATAAACTCAAATGGTAATTTTAGTTTGAATTTGTCCTCATGTGAAGACCAAATGATAAACAACTCTCTCTCTTCTTCTGGATTTCATGGAACTTCTTTTGAGGATACTTTTGCTGGAAATATTCTTCATTCAAATCAAGAACATAATATCAATCATGATGGTAATAATGATATTACAAAATCAACCACCAATGATGATGACGGTGCCGGTGCCGGAGGAAACAATGCACTTACAAGAGATTTCTTAGGTCTTAAACCACTCTCTGATAGTGATATCCTCACCATTGCTGGTATGGGAAGTTGCATGAACCCTTCCAACTCCGATCATCAAGCAAACCATTCCCAAAAACCATGGGAAGGTAATTAG
Protein sequence

>MS.gene76144.t1

MIKGFMSQQEQQSLVVEENMSNLTSASGEASANSSGNRTEVDAKFSQEYFASSQTQTHDETPAKKRRNLPGNPDPQAEVIALSPKTLMATNRFICEICNKGFQRDQNLQLHKRGHNLPWKLKQRTSNEIRKKVYVCPEPTCVHHDPSRALGDLTGIKKHFCRKHGEKKWKCDKCSKKYAVQSDWKAHSKTCGTREYRCDCGTLFSRRDSFITHRAFCDALAEESSRTVIPQPTQQNSHHNMNNLQTQDIQGLTLKKEHQSFNMLRPEQEVQIPSWLCQSNIDLSSNYSRLDQDLHLYENPNPRNGPTLPSYQPASAASPHMSATALLQKAAQMGATSSSQSMLSGTHQQGHVSIVDSATNNMINSNGNFSLNLSSCEDQMINNSLSSSGFHGTSFEDTFAGNILHSNQEHNINHDGNNDITKSTTNDDDGAGAGGNNALTRDFLGLKPLSDSDILTIAGMGSCMNPSNSDHQANHSQKPWEGN