Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76255.t1 | XP_013470240.1 | 91.4 | 186 | 7 | 3 | 1 | 181 | 1 | 182 | 1.40E-86 | 328.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76255.t1 | Q9FMC8 | 48.8 | 209 | 63 | 9 | 1 | 181 | 1 | 193 | 1.9e-31 | 137.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76255.t1 | A0A072VS70 | 91.4 | 186 | 7 | 3 | 1 | 181 | 1 | 182 | 1.0e-86 | 328.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene76255.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76255.t1 | MTR_1g111640 | 91.398 | 186 | 7 | 3 | 1 | 181 | 1 | 182 | 2.74e-119 | 338 |
| MS.gene76255.t1 | MTR_7g105780 | 49.239 | 197 | 69 | 9 | 1 | 182 | 1 | 181 | 1.70e-46 | 154 |
| MS.gene76255.t1 | MTR_1g058000 | 38.860 | 193 | 84 | 7 | 6 | 182 | 1 | 175 | 6.52e-29 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76255.t1 | AT5G04820 | 50.718 | 209 | 59 | 9 | 1 | 181 | 1 | 193 | 1.54e-48 | 158 |
| MS.gene76255.t1 | AT2G36050 | 47.423 | 97 | 33 | 5 | 95 | 181 | 66 | 154 | 1.18e-17 | 78.2 |
| MS.gene76255.t1 | AT3G52540 | 50.000 | 92 | 38 | 4 | 95 | 181 | 93 | 181 | 3.08e-15 | 72.0 |
Find 28 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTATAATGTTCTCTGTT+TGG | 0.328276 | 1.1:+78786594 | None:intergenic |
| AACACAACACTTTCTTTGAA+TGG | 0.349626 | 1.1:+78786374 | None:intergenic |
| CTACAACGACGGCGAATCAT+TGG | 0.389604 | 1.1:-78786501 | MS.gene76255:CDS |
| GATCTCTTAAAATCTTCATA+TGG | 0.409145 | 1.1:+78786332 | None:intergenic |
| AGTCTCATGGTGTGAAAGAT+TGG | 0.409364 | 1.1:-78786292 | MS.gene76255:CDS |
| GATGTTGTGAACCATGATTT+TGG | 0.433746 | 1.1:+78786569 | None:intergenic |
| TGTGAAAGATTGGGAAGGTT+TGG | 0.450919 | 1.1:-78786282 | MS.gene76255:CDS |
| AGCTGGTATTTGAAAGTGAA+TGG | 0.468308 | 1.1:-78786248 | MS.gene76255:intron |
| TGGCAATTCTTACCCTCTTG+TGG | 0.498707 | 1.1:-78786698 | MS.gene76255:CDS |
| TAAGAGATCAATGGAAGAGA+TGG | 0.508969 | 1.1:-78786318 | MS.gene76255:CDS |
| AGAAAGTGTTGTGTTAGCTA+TGG | 0.521557 | 1.1:-78786366 | MS.gene76255:CDS |
| CACGAGTTCGATCTTAGAAA+AGG | 0.522622 | 1.1:-78786414 | MS.gene76255:CDS |
| TGATGATGACATATTCAAAA+CGG | 0.538193 | 1.1:-78786636 | MS.gene76255:CDS |
| TTATAGAAACACCAAAATCA+TGG | 0.542649 | 1.1:-78786580 | MS.gene76255:CDS |
| GTCTCATGGTGTGAAAGATT+GGG | 0.558996 | 1.1:-78786291 | MS.gene76255:CDS |
| TTAGAAAAGGCGAAAGCGAA+TGG | 0.571257 | 1.1:-78786401 | MS.gene76255:CDS |
| TGTTAAGTCGGAGAGGTCAT+CGG | 0.571491 | 1.1:-78786462 | MS.gene76255:CDS |
| CACAAAGAAAACAACATCCA+TGG | 0.572198 | 1.1:-78786718 | MS.gene76255:CDS |
| CGGCGAATCATTGGAGACAT+TGG | 0.582049 | 1.1:-78786492 | MS.gene76255:CDS |
| CAAGAGGGTAAGAATTGCCA+TGG | 0.585631 | 1.1:+78786701 | None:intergenic |
| TGAGAAACTTGCAGATTCAG+AGG | 0.589678 | 1.1:+78786547 | None:intergenic |
| TGTGTTAGCTATGGAGTCAG+AGG | 0.627955 | 1.1:-78786357 | MS.gene76255:CDS |
| GAAGAGATGGTTGAGTCTCA+TGG | 0.637125 | 1.1:-78786305 | MS.gene76255:CDS |
| ATTGGTACGAGGTGTTAAGT+CGG | 0.638901 | 1.1:-78786474 | MS.gene76255:CDS |
| CATGGTGTGAAAGATTGGGA+AGG | 0.649930 | 1.1:-78786287 | MS.gene76255:CDS |
| GATTACTATTGCTACAACGA+CGG | 0.655187 | 1.1:-78786512 | MS.gene76255:CDS |
| TACGAGGTGTTAAGTCGGAG+AGG | 0.688804 | 1.1:-78786469 | MS.gene76255:CDS |
| TCATTGGAGACATTGGTACG+AGG | 0.736058 | 1.1:-78786485 | MS.gene76255:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGTACAACAACAAAAAA+AGG | + | chr1.1:78786763-78786782 | None:intergenic | 20.0% |
| !!! | TTTTCTTTGTGAAATTTCTT+TGG | + | chr1.1:78786021-78786040 | None:intergenic | 20.0% |
| ! | GATCTCTTAAAATCTTCATA+TGG | + | chr1.1:78786419-78786438 | None:intergenic | 25.0% |
| ! | GTGAATGGTAAAAATAATCA+TGG | - | chr1.1:78786515-78786534 | MS.gene76255:CDS | 25.0% |
| ! | TTATAGAAACACCAAAATCA+TGG | - | chr1.1:78786168-78786187 | MS.gene76255:intron | 25.0% |
| ! | TTTCTATAATGTTCTCTGTT+TGG | + | chr1.1:78786157-78786176 | None:intergenic | 25.0% |
| !! | TGATGATGACATATTCAAAA+CGG | - | chr1.1:78786112-78786131 | MS.gene76255:intron | 25.0% |
| !!! | AATAATCATGGCTTTATTGT+TGG | - | chr1.1:78786527-78786546 | MS.gene76255:CDS | 25.0% |
| !!! | TGAAGATTTTAAGAGATCAA+TGG | - | chr1.1:78786421-78786440 | MS.gene76255:CDS | 25.0% |
| AACACAACACTTTCTTTGAA+TGG | + | chr1.1:78786377-78786396 | None:intergenic | 30.0% | |
| ! | ACTCGAAAAGAAAGAGTTTT+TGG | + | chr1.1:78786080-78786099 | None:intergenic | 30.0% |
| !! | GAAAAGAAAGAGTTTTTGGA+TGG | + | chr1.1:78786076-78786095 | None:intergenic | 30.0% |
| !!! | AAAACTCTTTCTTTTCGAGT+TGG | - | chr1.1:78786080-78786099 | MS.gene76255:intron | 30.0% |
| !!! | TCTTTGGTTTTGAAAAGAGA+AGG | + | chr1.1:78786005-78786024 | None:intergenic | 30.0% |
| AGCTGGTATTTGAAAGTGAA+TGG | - | chr1.1:78786500-78786519 | MS.gene76255:CDS | 35.0% | |
| CACAAAGAAAACAACATCCA+TGG | - | chr1.1:78786030-78786049 | MS.gene76255:intron | 35.0% | |
| GATTACTATTGCTACAACGA+CGG | - | chr1.1:78786236-78786255 | MS.gene76255:intron | 35.0% | |
| TAAGAGATCAATGGAAGAGA+TGG | - | chr1.1:78786430-78786449 | MS.gene76255:CDS | 35.0% | |
| TAGATACAAAGGTGAAGAAG+AGG | + | chr1.1:78786651-78786670 | None:intergenic | 35.0% | |
| TCACCTTTGTATCTATGTGA+GGG | - | chr1.1:78786656-78786675 | MS.gene76255:CDS | 35.0% | |
| TTCACCTTTGTATCTATGTG+AGG | - | chr1.1:78786655-78786674 | MS.gene76255:CDS | 35.0% | |
| ! | GATGTTGTGAACCATGATTT+TGG | + | chr1.1:78786182-78786201 | None:intergenic | 35.0% |
| !! | AGAAAGTGTTGTGTTAGCTA+TGG | - | chr1.1:78786382-78786401 | MS.gene76255:CDS | 35.0% |
| AGTCTCATGGTGTGAAAGAT+TGG | - | chr1.1:78786456-78786475 | MS.gene76255:CDS | 40.0% | |
| CACGAGTTCGATCTTAGAAA+AGG | - | chr1.1:78786334-78786353 | MS.gene76255:CDS | 40.0% | |
| GTCTCATGGTGTGAAAGATT+GGG | - | chr1.1:78786457-78786476 | MS.gene76255:CDS | 40.0% | |
| TGAGAAACTTGCAGATTCAG+AGG | + | chr1.1:78786204-78786223 | None:intergenic | 40.0% | |
| TGTCCCTCACATAGATACAA+AGG | + | chr1.1:78786662-78786681 | None:intergenic | 40.0% | |
| TGTGAAAGATTGGGAAGGTT+TGG | - | chr1.1:78786466-78786485 | MS.gene76255:CDS | 40.0% | |
| TTAGAAAAGGCGAAAGCGAA+TGG | - | chr1.1:78786347-78786366 | MS.gene76255:CDS | 40.0% | |
| ! | ATTGGTACGAGGTGTTAAGT+CGG | - | chr1.1:78786274-78786293 | MS.gene76255:CDS | 40.0% |
| !!! | ACTCTTTCTTTTCGAGTTGG+TGG | - | chr1.1:78786083-78786102 | MS.gene76255:intron | 40.0% |
| CAAGAGGGTAAGAATTGCCA+TGG | + | chr1.1:78786050-78786069 | None:intergenic | 45.0% | |
| CATGGTGTGAAAGATTGGGA+AGG | - | chr1.1:78786461-78786480 | MS.gene76255:CDS | 45.0% | |
| GAAGAGATGGTTGAGTCTCA+TGG | - | chr1.1:78786443-78786462 | MS.gene76255:CDS | 45.0% | |
| TCATTGGAGACATTGGTACG+AGG | - | chr1.1:78786263-78786282 | MS.gene76255:CDS | 45.0% | |
| TGGCAATTCTTACCCTCTTG+TGG | - | chr1.1:78786050-78786069 | MS.gene76255:intron | 45.0% | |
| TGTGTTAGCTATGGAGTCAG+AGG | - | chr1.1:78786391-78786410 | MS.gene76255:CDS | 45.0% | |
| TGTTAAGTCGGAGAGGTCAT+CGG | - | chr1.1:78786286-78786305 | MS.gene76255:CDS | 45.0% | |
| ! | GTTTGGAAGAGCTTTTGAGC+TGG | - | chr1.1:78786483-78786502 | MS.gene76255:CDS | 45.0% |
| !! | AGTTTTTGGATGGCCACAAG+AGG | + | chr1.1:78786066-78786085 | None:intergenic | 45.0% |
| !! | GTTTTTGGATGGCCACAAGA+GGG | + | chr1.1:78786065-78786084 | None:intergenic | 45.0% |
| CGGCGAATCATTGGAGACAT+TGG | - | chr1.1:78786256-78786275 | MS.gene76255:CDS | 50.0% | |
| CTACAACGACGGCGAATCAT+TGG | - | chr1.1:78786247-78786266 | MS.gene76255:intron | 50.0% | |
| ! | TACGAGGTGTTAAGTCGGAG+AGG | - | chr1.1:78786279-78786298 | MS.gene76255:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 78785978 | 78786792 | 78785978 | ID=MS.gene76255 |
| chr1.1 | mRNA | 78785978 | 78786792 | 78785978 | ID=MS.gene76255.t1;Parent=MS.gene76255 |
| chr1.1 | exon | 78786249 | 78786792 | 78786249 | ID=MS.gene76255.t1.exon1;Parent=MS.gene76255.t1 |
| chr1.1 | CDS | 78786249 | 78786792 | 78786249 | ID=cds.MS.gene76255.t1;Parent=MS.gene76255.t1 |
| chr1.1 | exon | 78785978 | 78785982 | 78785978 | ID=MS.gene76255.t1.exon2;Parent=MS.gene76255.t1 |
| chr1.1 | CDS | 78785978 | 78785982 | 78785978 | ID=cds.MS.gene76255.t1;Parent=MS.gene76255.t1 |
| Gene Sequence |
| Protein sequence |
