AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76308

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76308.t1 MTR_1g110710 85.121 289 14 2 3 262 86 374 0.0 504
MS.gene76308.t1 MTR_1g110740 42.982 228 57 11 14 209 7 193 1.07e-31 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76308.t1 AT1G68480 43.458 214 87 5 15 205 2 204 6.25e-42 144
MS.gene76308.t1 AT1G13400 41.262 206 91 9 17 208 5 194 9.89e-29 108

Find 81 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCAAATTGTTAAGATCTAA+TGG 0.262058 1.1:+78160386 None:intergenic
TTGATCAATGCTGCTGAATT+AGG 0.273273 1.1:+78158435 None:intergenic
AATTGGGGAAGGAGCTATTC+AGG 0.281026 1.1:-78158498 MS.gene76308:CDS
CATTACTTTCTATGATCATT+TGG 0.293484 1.1:-78160965 None:intergenic
CCCACATGATATTCATTCAT+TGG 0.354688 1.1:+78158690 None:intergenic
TATGGAAGGGACATTGAATT+GGG 0.356851 1.1:-78158514 MS.gene76308:CDS
TTAATTTGTTGGCAGGTTGC+AGG 0.357498 1.1:-78159778 MS.gene76308:intron
CTAGCTAACTTCGTCTGGTT+TGG 0.358337 1.1:+78160927 None:intergenic
TCTGTAAATCTCAGGCTCTT+GGG 0.385516 1.1:-78159679 MS.gene76308:CDS
GGAATAAAACCTTGTCTAAC+CGG 0.387416 1.1:+78158570 None:intergenic
CCAGCTCCCTCTCACCTTGT+AGG 0.387947 1.1:-78159293 MS.gene76308:CDS
TCCTTCCCACCACTACTTCC+TGG 0.390896 1.1:+78158549 None:intergenic
CCTAGCTAGCTAACTTCGTC+TGG 0.391677 1.1:+78160922 None:intergenic
TGATCTCCCATGTTATTTGC+TGG 0.392930 1.1:+78158861 None:intergenic
ATATGGAAGGGACATTGAAT+TGG 0.404608 1.1:-78158515 MS.gene76308:CDS
TTCTGTAAATCTCAGGCTCT+TGG 0.411398 1.1:-78159680 MS.gene76308:CDS
GCAAATTGTTAAGATCTAAT+GGG 0.416673 1.1:+78160387 None:intergenic
GATGAACCTGAGAAATATCT+TGG 0.416710 1.1:+78158819 None:intergenic
CACAACATGAACTATGTTGC+AGG 0.424362 1.1:-78158633 MS.gene76308:CDS
ATATAGCTTGCTGTTGGTAT+TGG 0.426802 1.1:+78158885 None:intergenic
CGGGTAGATCAGTGTCTTCA+AGG 0.439966 1.1:+78160330 None:intergenic
AAATATCTTGGGAATCTCAT+AGG 0.445139 1.1:+78158831 None:intergenic
ATTCAGCAGCATTGATCAAT+CGG 0.448010 1.1:-78158431 MS.gene76308:CDS
AGCTATTCAGGAGGAGGAGG+AGG 0.463489 1.1:-78158486 MS.gene76308:CDS
GATGAATACTCTAGAGATCA+TGG 0.470780 1.1:-78160362 MS.gene76308:CDS
TAGCTTGCTGTTGGTATTGG+TGG 0.471374 1.1:+78158888 None:intergenic
CTGTAAATCTCAGGCTCTTG+GGG 0.476136 1.1:-78159678 MS.gene76308:CDS
AGTTCATGTTGTGATATTGC+TGG 0.481239 1.1:+78158643 None:intergenic
AGATTCCCAAGATATTTCTC+AGG 0.488336 1.1:-78158825 MS.gene76308:intron
AGGAGCTATTCAGGAGGAGG+AGG 0.489907 1.1:-78158489 MS.gene76308:CDS
CCTCAAGTTCTGTAAATCTC+AGG 0.490774 1.1:-78159687 MS.gene76308:CDS
CAGCTCCCTCTCACCTTGTA+GGG 0.493565 1.1:-78159292 MS.gene76308:intron
GAAGGATCATCATCAAGATA+TGG 0.494180 1.1:-78158532 MS.gene76308:CDS
CTTGCTGTTGGTATTGGTGG+TGG 0.494554 1.1:+78158891 None:intergenic
ATGAACCTGAGAAATATCTT+GGG 0.500848 1.1:+78158820 None:intergenic
TGATCTCTAGAGTATTCATC+AGG 0.517140 1.1:+78160365 None:intergenic
AGGTGAGAGGGAGCTGGTTG+TGG 0.522713 1.1:+78159299 None:intergenic
CTGAGATTTACAGAACTTGA+GGG 0.524443 1.1:+78159688 None:intergenic
TGTTGTGATATTGCTGGTGG+TGG 0.527644 1.1:+78158649 None:intergenic
TTGATCAATCGGTTTCAAGA+TGG 0.529982 1.1:-78158420 MS.gene76308:CDS
ATAGCTAGCCTTGGCGGTGA+CGG 0.544615 1.1:+78159645 None:intergenic
AAAAGAATAGCTAGCCTTGG+CGG 0.547654 1.1:+78159639 None:intergenic
GTGCAAGGTTATGATCGTTA+CGG 0.551633 1.1:+78159321 None:intergenic
ATGAACTATGTTGCAGGTGC+AGG 0.551767 1.1:-78158627 MS.gene76308:CDS
CTATATTCCAGCAAATAACA+TGG 0.555865 1.1:-78158868 MS.gene76308:CDS
GCTGGAATATAGCTTGCTGT+TGG 0.559096 1.1:+78158879 None:intergenic
TCCAATGAATGAATATCATG+TGG 0.567802 1.1:-78158691 MS.gene76308:CDS
TTTATTCCAGGAAGTAGTGG+TGG 0.567911 1.1:-78158555 MS.gene76308:CDS
CATTGGATACATACCCTACA+AGG 0.569471 1.1:+78159279 None:intergenic
TCCAGGAAGTAGTGGTGGGA+AGG 0.571603 1.1:-78158550 MS.gene76308:CDS
ATGGAAGGGACATTGAATTG+GGG 0.575489 1.1:-78158513 MS.gene76308:CDS
AGGATGGAAAAGATGAGTGT+GGG 0.577946 1.1:-78159736 MS.gene76308:CDS
TATGTTGCAGGTGCAGGTGG+TGG 0.578820 1.1:-78158621 MS.gene76308:CDS
GATCATCATCAAGATATGGA+AGG 0.580590 1.1:-78158528 MS.gene76308:CDS
CCTGAGATTTACAGAACTTG+AGG 0.580726 1.1:+78159687 None:intergenic
TATTCATTCATTGGATGCTG+AGG 0.581889 1.1:+78158699 None:intergenic
AACTATGTTGCAGGTGCAGG+TGG 0.582825 1.1:-78158624 MS.gene76308:CDS
AAGGATGGAAAAGATGAGTG+TGG 0.583920 1.1:-78159737 MS.gene76308:CDS
CCTACAAGGTGAGAGGGAGC+TGG 0.588325 1.1:+78159293 None:intergenic
CACATGAACCGTCACCGCCA+AGG 0.589189 1.1:-78159653 MS.gene76308:intron
TTACACTTGCATAGGTGCAC+CGG 0.591451 1.1:-78158589 MS.gene76308:CDS
GAGTCGAGTTACACTTGCAT+AGG 0.593277 1.1:-78158597 MS.gene76308:CDS
AGATCTAATGGGTTTCCTTG+CGG 0.593490 1.1:+78160398 None:intergenic
ATCATCATCAAGATATGGAA+GGG 0.601576 1.1:-78158527 MS.gene76308:CDS
GGAAGGAGCTATTCAGGAGG+AGG 0.605635 1.1:-78158492 MS.gene76308:CDS
GCTGAGGAATCAAATGTGTA+GGG 0.605957 1.1:+78158715 None:intergenic
AGGGAGCTGGTTGTGGTGCA+AGG 0.607305 1.1:+78159306 None:intergenic
TGGAAAAGATGAGTGTGGGA+AGG 0.610222 1.1:-78159732 MS.gene76308:CDS
ATAGGTGCACCGGTTAGACA+AGG 0.620734 1.1:-78158579 MS.gene76308:CDS
TTATTCCAGGAAGTAGTGGT+GGG 0.630975 1.1:-78158554 MS.gene76308:CDS
CTTGAAGACACTGATCTACC+CGG 0.633510 1.1:-78160329 MS.gene76308:intron
TATATTCCAGCAAATAACAT+GGG 0.637814 1.1:-78158867 MS.gene76308:CDS
TGCTGAGGAATCAAATGTGT+AGG 0.639346 1.1:+78158714 None:intergenic
GGAAGACTAGCTGACGTGCA+TGG 0.639350 1.1:+78159342 None:intergenic
TACATACCCTACAAGGTGAG+AGG 0.640151 1.1:+78159286 None:intergenic
ACATACCCTACAAGGTGAGA+GGG 0.643709 1.1:+78159287 None:intergenic
TCATGTTGTGATATTGCTGG+TGG 0.643930 1.1:+78158646 None:intergenic
AAGGGACATTGAATTGGGGA+AGG 0.649883 1.1:-78158509 MS.gene76308:CDS
CCAATGAATGAATATCATGT+GGG 0.659875 1.1:-78158690 MS.gene76308:CDS
TGTAAATCTCAGGCTCTTGG+GGG 0.671796 1.1:-78159677 MS.gene76308:CDS
TGGGGAAGGAGCTATTCAGG+AGG 0.735271 1.1:-78158495 MS.gene76308:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTATATTTTATTTT+TGG - chr1.1:78159315-78159334 MS.gene76308:CDS 0.0%
!!! AAAGTTAAATAAATTTTTAC+CGG + chr1.1:78159060-78159079 None:intergenic 10.0%
!! AAAATCATAACAATAGTATT+AGG - chr1.1:78160335-78160354 MS.gene76308:CDS 15.0%
!! AACAATTTAATAAGAAAGAA+AGG + chr1.1:78159349-78159368 None:intergenic 15.0%
!! ACAATTTAATAAGAAAGAAA+GGG + chr1.1:78159348-78159367 None:intergenic 15.0%
!! AGAGGAAAAATAAATAAAAA+GGG - chr1.1:78159446-78159465 MS.gene76308:intron 15.0%
!! CTAATTTATTTGTATGATAA+AGG + chr1.1:78160174-78160193 None:intergenic 15.0%
!! TAAATAAAGATCTGATTTAA+AGG + chr1.1:78159773-78159792 None:intergenic 15.0%
!! TAGAGGAAAAATAAATAAAA+AGG - chr1.1:78159445-78159464 MS.gene76308:intron 15.0%
!! TCATACAAATAAATTAGAAT+CGG - chr1.1:78160177-78160196 MS.gene76308:intron 15.0%
!!! AAAGTAAAAACATTTGATTT+AGG + chr1.1:78160282-78160301 None:intergenic 15.0%
!!! AAGTAAAAACATTTGATTTA+GGG + chr1.1:78160281-78160300 None:intergenic 15.0%
!!! AAGTTAAATAAATTTTTACC+GGG + chr1.1:78159059-78159078 None:intergenic 15.0%
!!! ATAATTTGATGATGATATAT+GGG - chr1.1:78159500-78159519 MS.gene76308:intron 15.0%
!!! GAAATATATGAATTGATTTA+GGG + chr1.1:78160384-78160403 None:intergenic 15.0%
!!! TATAATTTGATGATGATATA+TGG - chr1.1:78159499-78159518 MS.gene76308:intron 15.0%
!!! TGAAATATATGAATTGATTT+AGG + chr1.1:78160385-78160404 None:intergenic 15.0%
!! AAAGAAAATGGAACTATAAA+TGG + chr1.1:78160444-78160463 None:intergenic 20.0%
!! AAAGTATAATAAGTCTTTCA+TGG + chr1.1:78159202-78159221 None:intergenic 20.0%
!! AAGATAAATATGAATGAGAA+AGG + chr1.1:78158868-78158887 None:intergenic 20.0%
!! ACCCAAAAATAAAATAATGA+GGG - chr1.1:78159808-78159827 MS.gene76308:intron 20.0%
!! ATATGTGTAGATGAAATTAA+AGG + chr1.1:78160151-78160170 None:intergenic 20.0%
!! CTTCAAATATATTGTCATAA+TGG - chr1.1:78159413-78159432 MS.gene76308:intron 20.0%
!! GCTTGAAAATTAAAAGTAAT+TGG + chr1.1:78160315-78160334 None:intergenic 20.0%
!! GTATTAATTAAAGGAAAAAG+TGG + chr1.1:78160118-78160137 None:intergenic 20.0%
!! GTGATTATGGAATATAATAT+AGG + chr1.1:78159174-78159193 None:intergenic 20.0%
!!! ATATATTGTCATAATGGAAA+AGG - chr1.1:78159419-78159438 MS.gene76308:intron 20.0%
!!! ATCCCTCATTATTTTATTTT+TGG + chr1.1:78159813-78159832 None:intergenic 20.0%
!!! TCATTATTTTATTTTTGGGT+GGG + chr1.1:78159808-78159827 None:intergenic 20.0%
!!! TCCCTCATTATTTTATTTTT+GGG + chr1.1:78159812-78159831 None:intergenic 20.0%
! ACAACATCTGAAAAAGAAAA+TGG + chr1.1:78160456-78160475 None:intergenic 25.0%
! ATGATGATATATGGGAAATT+GGG - chr1.1:78159508-78159527 MS.gene76308:intron 25.0%
! ATGGAAAAAGATAAAAGAGA+AGG + chr1.1:78159374-78159393 None:intergenic 25.0%
! CACAAAATTGAATGAACAAT+AGG + chr1.1:78158566-78158585 None:intergenic 25.0%
! CACCCAAAAATAAAATAATG+AGG - chr1.1:78159807-78159826 MS.gene76308:intron 25.0%
! GATGTGTATATATTTGTGTT+GGG - chr1.1:78159965-78159984 MS.gene76308:intron 25.0%
! GCAAATTGTTAAGATCTAAT+GGG + chr1.1:78158983-78159002 None:intergenic 25.0%
! GGCATCATGATATAAATTAA+TGG - chr1.1:78160356-78160375 MS.gene76308:CDS 25.0%
! TATATTCCAGCAAATAACAT+GGG - chr1.1:78160500-78160519 MS.gene76308:intron 25.0%
! TATTTCATGTATGTCCATTA+TGG - chr1.1:78160398-78160417 MS.gene76308:CDS 25.0%
! TATTTGTGTTGGGTTTAATT+TGG - chr1.1:78159975-78159994 MS.gene76308:intron 25.0%
! TGATGTGTATATATTTGTGT+TGG - chr1.1:78159964-78159983 MS.gene76308:intron 25.0%
!! AAATTGGGAATGTTGATTAA+GGG - chr1.1:78159523-78159542 MS.gene76308:intron 25.0%
!! AGGAGCAAAGTATTAATTAA+AGG + chr1.1:78160127-78160146 None:intergenic 25.0%
!! GAAGTACTAAAATTCTTTCT+TGG - chr1.1:78159901-78159920 MS.gene76308:intron 25.0%
!! TATCTATTTGTTCCATTTTC+AGG - chr1.1:78158943-78158962 MS.gene76308:intron 25.0%
!!! CTCATTATTTTATTTTTGGG+TGG + chr1.1:78159809-78159828 None:intergenic 25.0%
!!! GGATATTGTTGTTAATTTGT+TGG - chr1.1:78159578-78159597 MS.gene76308:intron 25.0%
!!! TTTCTTGGTTTTGATTTGAA+AGG - chr1.1:78159916-78159935 MS.gene76308:intron 25.0%
AAATATCTTGGGAATCTCAT+AGG + chr1.1:78160539-78160558 None:intergenic 30.0%
AGACTTATTATACTTTCCAC+AGG - chr1.1:78159206-78159225 MS.gene76308:intron 30.0%
ATCATCATCAAGATATGGAA+GGG - chr1.1:78160840-78160859 MS.gene76308:intron 30.0%
ATGAACCTGAGAAATATCTT+GGG + chr1.1:78160550-78160569 None:intergenic 30.0%
ATGGTCAAAAGAATATACCA+CGG + chr1.1:78159266-78159285 None:intergenic 30.0%
CCAATGAATGAATATCATGT+GGG - chr1.1:78160677-78160696 MS.gene76308:intron 30.0%
CTATATTCCAGCAAATAACA+TGG - chr1.1:78160499-78160518 MS.gene76308:intron 30.0%
GATGATGATATATGGGAAAT+TGG - chr1.1:78159507-78159526 MS.gene76308:intron 30.0%
GGAACAAATAGATATAGTGA+AGG + chr1.1:78158937-78158956 None:intergenic 30.0%
GGCAAATTGTTAAGATCTAA+TGG + chr1.1:78158984-78159003 None:intergenic 30.0%
GTGTAGATGAAATTAAAGGA+AGG + chr1.1:78160147-78160166 None:intergenic 30.0%
TACAAAACAATGAGCCATAA+TGG + chr1.1:78160415-78160434 None:intergenic 30.0%
TCCAATGAATGAATATCATG+TGG - chr1.1:78160676-78160695 MS.gene76308:intron 30.0%
TTTAAAGAAGCTAGCAAGTA+TGG + chr1.1:78159393-78159412 None:intergenic 30.0%
TTTCATGGTATGTGTGATTA+TGG + chr1.1:78159187-78159206 None:intergenic 30.0%
! AATTATTGAGCACTGTTTCA+TGG + chr1.1:78159285-78159304 None:intergenic 30.0%
! ATCTTCCCTTCTAAAAGAAA+GGG - chr1.1:78158763-78158782 MS.gene76308:intron 30.0%
! GAAATTGGGAATGTTGATTA+AGG - chr1.1:78159522-78159541 MS.gene76308:intron 30.0%
! TAAACACCCTTTCTTTTAGA+AGG + chr1.1:78158772-78158791 None:intergenic 30.0%
! TATCTTCCCTTCTAAAAGAA+AGG - chr1.1:78158762-78158781 MS.gene76308:intron 30.0%
! TGAAAGTCTCACAAATTTTG+TGG - chr1.1:78158726-78158745 MS.gene76308:intron 30.0%
!! AAACACCCTTTCTTTTAGAA+GGG + chr1.1:78158771-78158790 None:intergenic 30.0%
!! ATTGTTGTTAATTTGTTGGC+AGG - chr1.1:78159582-78159601 MS.gene76308:intron 30.0%
AACCCACCGTACTAAATTAA+TGG - chr1.1:78160253-78160272 MS.gene76308:intron 35.0%
AGAAGGGAAGATAAAACTAG+AGG + chr1.1:78158755-78158774 None:intergenic 35.0%
AGAATCGGATAAGATGTTGT+GGG - chr1.1:78160192-78160211 MS.gene76308:intron 35.0%
AGATTCCCAAGATATTTCTC+AGG - chr1.1:78160542-78160561 MS.gene76308:intron 35.0%
AGGGCTCCATTAATTTAGTA+CGG + chr1.1:78160262-78160281 None:intergenic 35.0%
AGTTCATGTTGTGATATTGC+TGG + chr1.1:78160727-78160746 None:intergenic 35.0%
ATATGGAAGGGACATTGAAT+TGG - chr1.1:78160852-78160871 MS.gene76308:intron 35.0%
CCCACATGATATTCATTCAT+TGG + chr1.1:78160680-78160699 None:intergenic 35.0%
CTCCATTAATTTAGTACGGT+GGG + chr1.1:78160258-78160277 None:intergenic 35.0%
CTGAGATTTACAGAACTTGA+GGG + chr1.1:78159682-78159701 None:intergenic 35.0%
GAAGGATCATCATCAAGATA+TGG - chr1.1:78160835-78160854 MS.gene76308:intron 35.0%
GATCATCATCAAGATATGGA+AGG - chr1.1:78160839-78160858 MS.gene76308:intron 35.0%
GATGAACCTGAGAAATATCT+TGG + chr1.1:78160551-78160570 None:intergenic 35.0%
GGAATAAAACCTTGTCTAAC+CGG + chr1.1:78160800-78160819 None:intergenic 35.0%
GGTTGCAGGAAAAAGAAAAA+CGG - chr1.1:78159603-78159622 MS.gene76308:intron 35.0%
TAGAATCGGATAAGATGTTG+TGG - chr1.1:78160191-78160210 MS.gene76308:intron 35.0%
TATGGAAGGGACATTGAATT+GGG - chr1.1:78160853-78160872 MS.gene76308:intron 35.0%
TATTCATTCATTGGATGCTG+AGG + chr1.1:78160671-78160690 None:intergenic 35.0%
TGGTCAAAAGAATATACCAC+GGG + chr1.1:78159265-78159284 None:intergenic 35.0%
TTGAATGAACAATAGGACAG+TGG + chr1.1:78158559-78158578 None:intergenic 35.0%
TTGATCAATGCTGCTGAATT+AGG + chr1.1:78160935-78160954 None:intergenic 35.0%
! AGTTTGAGACTGCAATATAG+AGG + chr1.1:78158707-78158726 None:intergenic 35.0%
! ATGAAAAGAATAGCTAGCCT+TGG + chr1.1:78159734-78159753 None:intergenic 35.0%
! ATGAGAAAGGTGTAGTATAG+TGG + chr1.1:78158855-78158874 None:intergenic 35.0%
! CATAATGGAAAAGGAGTTAG+AGG - chr1.1:78159428-78159447 MS.gene76308:intron 35.0%
! GATGAATACTCTAGAGATCA+TGG - chr1.1:78159005-78159024 MS.gene76308:intron 35.0%
! TCAATTTTGTGTGAGTGTGT+GGG - chr1.1:78158575-78158594 MS.gene76308:CDS 35.0%
! TGAGAAAGGTGTAGTATAGT+GGG + chr1.1:78158854-78158873 None:intergenic 35.0%
! TGTTGATTAAGGGAAAGTAG+TGG - chr1.1:78159533-78159552 MS.gene76308:intron 35.0%
! TTCAATTTTGTGTGAGTGTG+TGG - chr1.1:78158574-78158593 MS.gene76308:CDS 35.0%
! TTGATCAATCGGTTTCAAGA+TGG - chr1.1:78160947-78160966 MS.gene76308:CDS 35.0%
!! ATATAGCTTGCTGTTGGTAT+TGG + chr1.1:78160485-78160504 None:intergenic 35.0%
!! ATTCAGCAGCATTGATCAAT+CGG - chr1.1:78160936-78160955 MS.gene76308:CDS 35.0%
!! GTGTTCAATTTTGTTTCCTG+TGG + chr1.1:78159225-78159244 None:intergenic 35.0%
!! TGATCTCTAGAGTATTCATC+AGG + chr1.1:78159005-78159024 None:intergenic 35.0%
!!! GTTAGACAAGGTTTTATTCC+AGG - chr1.1:78160800-78160819 MS.gene76308:intron 35.0%
AAATGTGTAGGGAAAGACAC+TGG + chr1.1:78160644-78160663 None:intergenic 40.0%
AAGGAAAAAGTGGTGTGCAT+TGG + chr1.1:78160108-78160127 None:intergenic 40.0%
AAGGATGGAAAAGATGAGTG+TGG - chr1.1:78159630-78159649 MS.gene76308:intron 40.0%
AATGTGTAGGGAAAGACACT+GGG + chr1.1:78160643-78160662 None:intergenic 40.0%
AGGATGGAAAAGATGAGTGT+GGG - chr1.1:78159631-78159650 MS.gene76308:intron 40.0%
ATGGAAGGGACATTGAATTG+GGG - chr1.1:78160854-78160873 MS.gene76308:intron 40.0%
CACAACATGAACTATGTTGC+AGG - chr1.1:78160734-78160753 MS.gene76308:intron 40.0%
CATTGGATACATACCCTACA+AGG + chr1.1:78160091-78160110 None:intergenic 40.0%
CCTCAAGTTCTGTAAATCTC+AGG - chr1.1:78159680-78159699 MS.gene76308:CDS 40.0%
CCTGAGATTTACAGAACTTG+AGG + chr1.1:78159683-78159702 None:intergenic 40.0%
CTCGTCGAAGTTGCTTAAAT+AGG - chr1.1:78159557-78159576 MS.gene76308:intron 40.0%
GAAAGAAAGAGATTCGAGAG+AGG - chr1.1:78158618-78158637 MS.gene76308:CDS 40.0%
GAAAGGTCTTTCTCTTGTGT+TGG - chr1.1:78159933-78159952 MS.gene76308:intron 40.0%
GAGTACAGATATGGTTGAGA+TGG + chr1.1:78160608-78160627 None:intergenic 40.0%
GCTCCATTAATTTAGTACGG+TGG + chr1.1:78160259-78160278 None:intergenic 40.0%
GCTGAGGAATCAAATGTGTA+GGG + chr1.1:78160655-78160674 None:intergenic 40.0%
GGTCAAAAGAATATACCACG+GGG + chr1.1:78159264-78159283 None:intergenic 40.0%
GTGCAAGGTTATGATCGTTA+CGG + chr1.1:78160049-78160068 None:intergenic 40.0%
TAAACAGCTGTGAGATGAGT+GGG + chr1.1:78160234-78160253 None:intergenic 40.0%
TCATGTTGTGATATTGCTGG+TGG + chr1.1:78160724-78160743 None:intergenic 40.0%
TCTGTAAATCTCAGGCTCTT+GGG - chr1.1:78159688-78159707 MS.gene76308:CDS 40.0%
TGACATGTGACAACGTTTCA+TGG + chr1.1:78159862-78159881 None:intergenic 40.0%
TGCAGGAAAAAGAAAAACGG+CGG - chr1.1:78159606-78159625 MS.gene76308:intron 40.0%
TGCTGAGGAATCAAATGTGT+AGG + chr1.1:78160656-78160675 None:intergenic 40.0%
TTAAACAGCTGTGAGATGAG+TGG + chr1.1:78160235-78160254 None:intergenic 40.0%
TTATTCCAGGAAGTAGTGGT+GGG - chr1.1:78160813-78160832 MS.gene76308:intron 40.0%
TTCTGTAAATCTCAGGCTCT+TGG - chr1.1:78159687-78159706 MS.gene76308:CDS 40.0%
TTCTTTCTGTCTCTCTCTGT+CGG - chr1.1:78158808-78158827 MS.gene76308:intron 40.0%
TTTATTCCAGGAAGTAGTGG+TGG - chr1.1:78160812-78160831 MS.gene76308:intron 40.0%
! AATTTTGTGTGAGTGTGTGG+GGG - chr1.1:78158577-78158596 MS.gene76308:CDS 40.0%
! AGAAAGGTGTAGTATAGTGG+GGG + chr1.1:78158852-78158871 None:intergenic 40.0%
! CAATTTTGTGTGAGTGTGTG+GGG - chr1.1:78158576-78158595 MS.gene76308:CDS 40.0%
! GAGAAAGGTGTAGTATAGTG+GGG + chr1.1:78158853-78158872 None:intergenic 40.0%
! GTAGGTGAAGAGTACAGATA+TGG + chr1.1:78160617-78160636 None:intergenic 40.0%
! TATAGAGGCTAGTTGAGTGA+TGG + chr1.1:78158692-78158711 None:intergenic 40.0%
! TGATCTCCCATGTTATTTGC+TGG + chr1.1:78160509-78160528 None:intergenic 40.0%
!! AAAAGAATAGCTAGCCTTGG+CGG + chr1.1:78159731-78159750 None:intergenic 40.0%
!! AGATCTAATGGGTTTCCTTG+CGG + chr1.1:78158972-78158991 None:intergenic 40.0%
!! GGTTTAATTTGGTGCAGAGA+GGG - chr1.1:78159986-78160005 MS.gene76308:intron 40.0%
!! TTAATTTGTTGGCAGGTTGC+AGG - chr1.1:78159589-78159608 MS.gene76308:intron 40.0%
!!! GGTTTTATTCCAGGAAGTAG+TGG - chr1.1:78160809-78160828 MS.gene76308:intron 40.0%
AAGGGACATTGAATTGGGGA+AGG - chr1.1:78160858-78160877 MS.gene76308:intron 45.0%
AATTGGGGAAGGAGCTATTC+AGG - chr1.1:78160869-78160888 MS.gene76308:intron 45.0%
ACATACCCTACAAGGTGAGA+GGG + chr1.1:78160083-78160102 None:intergenic 45.0%
AGAAAGAGATTCGAGAGAGG+AGG - chr1.1:78158621-78158640 MS.gene76308:CDS 45.0%
AGATATGGTTGAGATGGCTG+TGG + chr1.1:78160602-78160621 None:intergenic 45.0%
ATGAACTATGTTGCAGGTGC+AGG - chr1.1:78160740-78160759 MS.gene76308:intron 45.0%
CTAGCTAACTTCGTCTGGTT+TGG + chr1.1:78158443-78158462 None:intergenic 45.0%
CTCTGTCGGTACAATCATGT+AGG - chr1.1:78158822-78158841 MS.gene76308:intron 45.0%
CTGTAAATCTCAGGCTCTTG+GGG - chr1.1:78159689-78159708 MS.gene76308:CDS 45.0%
CTTGAAGACACTGATCTACC+CGG - chr1.1:78159038-78159057 MS.gene76308:intron 45.0%
GAAAAAGAAAAACGGCGGGA+AGG - chr1.1:78159611-78159630 MS.gene76308:intron 45.0%
GAGTCGAGTTACACTTGCAT+AGG - chr1.1:78160770-78160789 MS.gene76308:intron 45.0%
GCAGGAAAAAGAAAAACGGC+GGG - chr1.1:78159607-78159626 MS.gene76308:intron 45.0%
TACATACCCTACAAGGTGAG+AGG + chr1.1:78160084-78160103 None:intergenic 45.0%
TGGAAAAGATGAGTGTGGGA+AGG - chr1.1:78159635-78159654 MS.gene76308:intron 45.0%
TGTAAATCTCAGGCTCTTGG+GGG - chr1.1:78159690-78159709 MS.gene76308:CDS 45.0%
TGTTGTGATATTGCTGGTGG+TGG + chr1.1:78160721-78160740 None:intergenic 45.0%
TTACACTTGCATAGGTGCAC+CGG - chr1.1:78160778-78160797 MS.gene76308:intron 45.0%
! GTCATATTTTGTCATCCCCG+TGG - chr1.1:78159246-78159265 MS.gene76308:intron 45.0%
! TGAGATGAGTGGGAGTGTAT+GGG + chr1.1:78160224-78160243 None:intergenic 45.0%
!! GCTGGAATATAGCTTGCTGT+TGG + chr1.1:78160491-78160510 None:intergenic 45.0%
!! GGGTTTAATTTGGTGCAGAG+AGG - chr1.1:78159985-78160004 MS.gene76308:intron 45.0%
!! TAGCTTGCTGTTGGTATTGG+TGG + chr1.1:78160482-78160501 None:intergenic 45.0%
AACTATGTTGCAGGTGCAGG+TGG - chr1.1:78160743-78160762 MS.gene76308:intron 50.0%
AAGAAAAACGGCGGGAAGGA+TGG - chr1.1:78159615-78159634 MS.gene76308:intron 50.0%
AAGAGATTCGAGAGAGGAGG+TGG - chr1.1:78158624-78158643 MS.gene76308:CDS 50.0%
CCAGACGAAGTTAGCTAGCT+AGG - chr1.1:78158445-78158464 MS.gene76308:CDS 50.0%
CCTAGCTAGCTAACTTCGTC+TGG + chr1.1:78158448-78158467 None:intergenic 50.0%
CGGGTAGATCAGTGTCTTCA+AGG + chr1.1:78159040-78159059 None:intergenic 50.0%
TATGGTTGAGATGGCTGTGG+TGG + chr1.1:78160599-78160618 None:intergenic 50.0%
TCCTTGCGGTCTCCTGAAAA+TGG + chr1.1:78158958-78158977 None:intergenic 50.0%
! GTGAGATGAGTGGGAGTGTA+TGG + chr1.1:78160225-78160244 None:intergenic 50.0%
!! ATAGGTGCACCGGTTAGACA+AGG - chr1.1:78160788-78160807 MS.gene76308:intron 50.0%
!! CTTGCTGTTGGTATTGGTGG+TGG + chr1.1:78160479-78160498 None:intergenic 50.0%
!! TCCATTTTCAGGAGACCGCA+AGG - chr1.1:78158954-78158973 MS.gene76308:intron 50.0%
AGCTATTCAGGAGGAGGAGG+AGG - chr1.1:78160881-78160900 MS.gene76308:intron 55.0%
AGGAGCTATTCAGGAGGAGG+AGG - chr1.1:78160878-78160897 MS.gene76308:intron 55.0%
CAGCTCCCTCTCACCTTGTA+GGG - chr1.1:78160075-78160094 MS.gene76308:intron 55.0%
GGAAGGAGCTATTCAGGAGG+AGG - chr1.1:78160875-78160894 MS.gene76308:intron 55.0%
TATGTTGCAGGTGCAGGTGG+TGG - chr1.1:78160746-78160765 MS.gene76308:intron 55.0%
TCCTTCCCACCACTACTTCC+TGG + chr1.1:78160821-78160840 None:intergenic 55.0%
TGGGGAAGGAGCTATTCAGG+AGG - chr1.1:78160872-78160891 MS.gene76308:intron 55.0%
! GGAAGACTAGCTGACGTGCA+TGG + chr1.1:78160028-78160047 None:intergenic 55.0%
! TCCAGGAAGTAGTGGTGGGA+AGG - chr1.1:78160817-78160836 MS.gene76308:intron 55.0%
!! ATAGCTAGCCTTGGCGGTGA+CGG + chr1.1:78159725-78159744 None:intergenic 55.0%
!! TGGTGGTGGTGGTGGAATGT+GGG + chr1.1:78160579-78160598 None:intergenic 55.0%
AGGGAGCTGGTTGTGGTGCA+AGG + chr1.1:78160064-78160083 None:intergenic 60.0%
AGGTGAGAGGGAGCTGGTTG+TGG + chr1.1:78160071-78160090 None:intergenic 60.0%
CACATGAACCGTCACCGCCA+AGG - chr1.1:78159714-78159733 MS.gene76308:CDS 60.0%
CCAGCTCCCTCTCACCTTGT+AGG - chr1.1:78160074-78160093 MS.gene76308:intron 60.0%
CCTACAAGGTGAGAGGGAGC+TGG + chr1.1:78160077-78160096 None:intergenic 60.0%
GGGAAAGACACTGGGCGTGT+AGG + chr1.1:78160635-78160654 None:intergenic 60.0%
GGTTGAGATGGCTGTGGTGG+TGG + chr1.1:78160596-78160615 None:intergenic 60.0%
TTCGAGAGAGGAGGTGGCAG+AGG - chr1.1:78158630-78158649 MS.gene76308:CDS 60.0%
!! GTGGTGGTGGTGGTGGAATG+TGG + chr1.1:78160580-78160599 None:intergenic 60.0%
!! TGAGATGGCTGTGGTGGTGG+TGG + chr1.1:78160593-78160612 None:intergenic 60.0%
!! GATGGCTGTGGTGGTGGTGG+TGG + chr1.1:78160590-78160609 None:intergenic 65.0%
!! GGCTGTGGTGGTGGTGGTGG+TGG + chr1.1:78160587-78160606 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 78158413 78160976 78158413 ID=MS.gene76308
chr1.1 mRNA 78158413 78160976 78158413 ID=MS.gene76308.t1;Parent=MS.gene76308
chr1.1 exon 78160936 78160976 78160936 ID=MS.gene76308.t1.exon1;Parent=MS.gene76308.t1
chr1.1 CDS 78160936 78160976 78160936 ID=cds.MS.gene76308.t1;Parent=MS.gene76308.t1
chr1.1 exon 78160330 78160424 78160330 ID=MS.gene76308.t1.exon2;Parent=MS.gene76308.t1
chr1.1 CDS 78160330 78160424 78160330 ID=cds.MS.gene76308.t1;Parent=MS.gene76308.t1
chr1.1 exon 78159654 78159785 78159654 ID=MS.gene76308.t1.exon3;Parent=MS.gene76308.t1
chr1.1 CDS 78159654 78159785 78159654 ID=cds.MS.gene76308.t1;Parent=MS.gene76308.t1
chr1.1 exon 78159293 78159386 78159293 ID=MS.gene76308.t1.exon4;Parent=MS.gene76308.t1
chr1.1 CDS 78159293 78159386 78159293 ID=cds.MS.gene76308.t1;Parent=MS.gene76308.t1
chr1.1 exon 78158826 78158920 78158826 ID=MS.gene76308.t1.exon5;Parent=MS.gene76308.t1
chr1.1 CDS 78158826 78158920 78158826 ID=cds.MS.gene76308.t1;Parent=MS.gene76308.t1
chr1.1 exon 78158413 78158744 78158413 ID=MS.gene76308.t1.exon6;Parent=MS.gene76308.t1
chr1.1 CDS 78158413 78158744 78158413 ID=cds.MS.gene76308.t1;Parent=MS.gene76308.t1
Gene Sequence

>MS.gene76308

ATGATCATTTGGTTTCTTGAGTCTCAACCAAACCAGACGAAGTTAGCTAGCTAGGAATTAGCAAGTATCTGATTAAGCTAGCTTTAGCAATCTTCACTACTTTCACACACACACACACACTCTCTTTTTAAAGTCACGAGAAGCCACTGTCCTATTGTTCATTCAATTTTGTGTGAGTGTGTGGGGGAGATATAAGAGAGAGAAAGAAAGAAAGAGATTCGAGAGAGGAGGTGGCAGAGGAACTGATAGAGAACTTTCAAAGCTCTTTATCTTCTACCATCACTCAACTAGCCTCTATATTGCAGTCTCAAACTGAAAGTCTCACAAATTTTGTGGAAACCTCTAGTTTTATCTTCCCTTCTAAAAGAAAGGGTGTTTATCTCATATCATTTTTCTTCTTTCTGTCTCTCTCTGTCGGTACAATCATGTAGGTCTTCCCCCACTATACTACACCTTTCTCATTCATATTTATCTTCTTCTCTATAACTCTAACTCATTAATTAACTCTCTCTTTTCTCTCTCCTTCACTATATCTATTTGTTCCATTTTCAGGAGACCGCAAGGAAACCCATTAGATCTTAACAATTTGCCTGATGAATACTCTAGAGATCATGGTAAACAACTCCTTGAAGACACTGATCTACCCGGTAAAAATTTATTTAACTTTTTTTCTTCTTCTTCTTCTTAATTTAGTGATCAAATCATACAATATAAATATACACTGAAACATATATAAAAACAGCATAAAACTTGTTTGTCCTATATTATATTCCATAATCACACATACCATGAAAGACTTATTATACTTTCCACAGGAAACAAAATTGAACACTGTCATATTTTGTCATCCCCGTGGTATATTCTTTTGACCATGAAACAGTGCTCAATAATTAAATGTTTTTATTTTATTATATTTTATTTTTGGTTTAACTCCCTTTCTTTCTTATTAAATTGTTTTCCTTCTCTTTTATCTTTTTCCATACTTGCTAGCTTCTTTAAACTTCAAATATATTGTCATAATGGAAAAGGAGTTAGAGGAAAAATAAATAAAAAGGGCAGTGAGTAAATTAATAATAGTGCTAAATATATAATTTGATGATGATATATGGGAAATTGGGAATGTTGATTAAGGGAAAGTAGTGGTCTCGTCGAAGTTGCTTAAATAGGATATTGTTGTTAATTTGTTGGCAGGTTGCAGGAAAAAGAAAAACGGCGGGAAGGATGGAAAAGATGAGTGTGGGAAGGTCTACGAATGTAGATTCTGTTCCCTCAAGTTCTGTAAATCTCAGGCTCTTGGGGGACACATGAACCGTCACCGCCAAGGCTAGCTATTCTTTTCATTTTTTCAAATTAATTTCCTTTAAATCAGATCTTTATTTAAAATATATATATCCCACCCAAAAATAAAATAATGAGGGATACTCATACTAGTTGTTAATGATTTGTCCATGAAACGTTGTCACATGTCATTTCAAAAACAAAAGTTATGAAGTACTAAAATTCTTTCTTGGTTTTGATTTGAAAGGTCTTTCTCTTGTGTTGGCTTTGATTTGATGTGTATATATTTGTGTTGGGTTTAATTTGGTGCAGAGAGGGAAACTGAGACGCTTAACCATGCACGTCAGCTAGTCTTCCGTAACGATCATAACCTTGCACCACAACCAGCTCCCTCTCACCTTGTAGGGTATGTATCCAATGCACACCACTTTTTCCTTTAATTAATACTTTGCTCCTTCCTTTAATTTCATCTACACATATCCTTTATCATACAAATAAATTAGAATCGGATAAGATGTTGTGGGTTCAAACCCATACACTCCCACTCATCTCACAGCTGTTTAACCCACCGTACTAAATTAATGGAGCCCTAAATCAAATGTTTTTACTTTATTTTCTATACCAATTACTTTTAATTTTCAAGCAAAATCATAACAATAGTATTAGGCATCATGATATAAATTAATGGAACCCTAAATCAATTCATATATTTCATGTATGTCCATTATGGCTCATTGTTTTGTATACTTTCCATTTATAGTTCCATTTTCTTTTTCAGATGTTGTCCACCACCAATACCAACAGCAAGCTATATTCCAGCAAATAACATGGGAGATCAAACAATTCCTATGAGATTCCCAAGATATTTCTCAGGTTCATCATCAACCCACATTCCACCACCACCACCACCACAGCCATCTCAACCATATCTGTACTCTTCACCTACACGCCCAGTGTCTTTCCCTACACATTTGATTCCTCAGCATCCAATGAATGAATATCATGTGGGTCACGTCATGAGTAGCAGCCACCACCAGCAATATCACAACATGAACTATGTTGCAGGTGCAGGTGGTGGTGAGTCGAGTTACACTTGCATAGGTGCACCGGTTAGACAAGGTTTTATTCCAGGAAGTAGTGGTGGGAAGGATCATCATCAAGATATGGAAGGGACATTGAATTGGGGAAGGAGCTATTCAGGAGGAGGAGGAGGACAGCAGCATCGTTTAGATACAACAACTCCTAATTCAGCAGCATTGATCAATCGGTTTCAAGATGGTTTCTAA
Protein sequence

>MS.gene76308.t1

MIIWFLESQPNQTKRPQGNPLDLNNLPDEYSRDHGKQLLEDTDLPGCRKKKNGGKDGKDECGKVYECRFCSLKFCKSQALGGHMNRHRQERETETLNHARQLVFRNDHNLAPQPAPSHLVGCCPPPIPTASYIPANNMGDQTIPMRFPRYFSVSFPTHLIPQHPMNEYHVGHVMSSSHHQQYHNMNYVAGAGGGESSYTCIGAPVRQGFIPGSSGGKDHHQDMEGTLNWGRSYSGGGGGQQHRLDTTTPNSAALINRFQDGF