AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76502

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76502.t1 MTR_6g081160 40.426 282 89 7 185 397 1 272 2.22e-52 176
MS.gene76502.t1 MTR_6g081160 42.857 126 49 5 114 233 1 109 4.83e-17 80.9
MS.gene76502.t1 MTR_6g081160 65.000 60 17 1 20 75 1 60 5.19e-16 77.8
MS.gene76502.t1 MTR_5g098470 31.429 175 80 5 201 355 55 209 6.57e-14 71.6
MS.gene76502.t1 MTR_5g062300 25.957 235 135 7 8 233 3 207 4.25e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76502.t1 AT1G02030 27.132 258 95 8 41 232 1 231 6.51e-14 72.0
MS.gene76502.t1 AT5G56200 38.542 96 45 2 210 305 79 160 5.45e-13 70.9
MS.gene76502.t1 AT5G43170 29.114 158 80 2 80 233 12 141 5.87e-12 64.7
MS.gene76502.t1 AT2G17180 27.083 192 121 4 44 235 64 236 1.07e-11 65.5
MS.gene76502.t1 AT2G26940 37.143 105 61 1 210 309 49 153 1.23e-11 65.5
MS.gene76502.t1 AT3G19580 33.684 95 51 2 139 233 106 188 3.71e-11 63.9
MS.gene76502.t1 AT3G19580 33.684 95 51 2 139 233 106 188 3.71e-11 63.9
MS.gene76502.t1 AT3G60580 28.358 134 76 3 139 272 173 286 7.01e-11 63.2

Find 73 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCTCTCTTCTGCCATTT+TGG 0.136557 2.3:+2364033 None:intergenic
CAATACAAGGTAGTTATAAT+AGG 0.224337 2.3:-2364749 MS.gene76502:CDS
CAATACAAGGTAGTACTAAT+AGG 0.256706 2.3:-2364254 MS.gene76502:CDS
GTGATTTATCTTTGCCAAAA+TGG 0.304193 2.3:-2364047 MS.gene76502:CDS
GTCCTTTAAAATCTCTATTC+CGG 0.308881 2.3:+2364112 None:intergenic
CATGATGAAGTTTCTGCTTA+AGG 0.329845 2.3:+2364517 None:intergenic
GTGACCATACAAAGCATTCA+TGG 0.407800 2.3:+2364146 None:intergenic
CCAACCATGAATGCTTTGTA+TGG 0.410676 2.3:-2364150 MS.gene76502:CDS
AATACAAGGTAGTACTAATA+GGG 0.424373 2.3:-2364253 MS.gene76502:CDS
TACTACCTTGTATTGAGAAA+GGG 0.428907 2.3:+2364262 None:intergenic
AATACAAGGTAGTTATAATA+GGG 0.431054 2.3:-2364748 MS.gene76502:CDS
CACATCAGTGCAATGTTTGT+GGG 0.435235 2.3:-2364872 MS.gene76502:CDS
ACACATCAGTGCAATGTTTG+TGG 0.442286 2.3:-2364378 MS.gene76502:CDS
TTTAGCAATGGAAAAGCATT+GGG 0.444822 2.3:-2364843 MS.gene76502:CDS
TCACATCAGTGCAATGTTTG+TGG 0.451639 2.3:-2364873 MS.gene76502:CDS
GTACTACCTTGTATTGAGAA+AGG 0.452759 2.3:+2364261 None:intergenic
ACACATCAATGCAATGTTTG+TGG 0.453484 2.3:-2364591 MS.gene76502:CDS
TTTAGTAACGGAAAAGCATT+GGG 0.453684 2.3:-2364348 MS.gene76502:CDS
ATTCGGAAAAGAGGATGAAA+AGG 0.459648 2.3:-2364959 MS.gene76502:CDS
TGAGAAAACCTATGAAGATT+CGG 0.461549 2.3:-2364976 MS.gene76502:CDS
TTTAGCAACGGAAAAGCATT+GGG 0.462037 2.3:-2364561 MS.gene76502:CDS
TGTGTTGGTGAAACATCAAA+TGG 0.466991 2.3:-2363937 MS.gene76502:CDS
AGTCAGAAAAGAGGGTGAAA+AGG 0.473192 2.3:-2364464 MS.gene76502:CDS
GAATGAGTCGTATGTTATAG+AGG 0.473597 2.3:-2363836 MS.gene76502:CDS
GATGATGAAGAAGAAATTGA+TGG 0.474180 2.3:-2364213 MS.gene76502:CDS
AGAAGAGGTAGAAAGTGTAT+TGG 0.483023 2.3:-2364012 MS.gene76502:CDS
AGTCTGAAAAGAGGATAAAG+AGG 0.489348 2.3:-2364677 MS.gene76502:CDS
AGTGATAAGCAGCCAAAGAA+AGG 0.491245 2.3:-2363877 MS.gene76502:CDS
TTTGATAAAGATAGTAAGCA+TGG 0.491925 2.3:-2364720 MS.gene76502:CDS
AAGCAGCCAAAGAAAGGAAA+AGG 0.495623 2.3:-2363871 MS.gene76502:CDS
ACTTCATCATGATTCACAAA+AGG 0.507116 2.3:-2364505 MS.gene76502:CDS
ACTCATTCATCATCAACAAA+AGG 0.509294 2.3:-2364292 MS.gene76502:CDS
AACTACCTTGTATTGAGAGA+GGG 0.509993 2.3:+2364757 None:intergenic
CCTATGAAGATTCGGAAAAG+AGG 0.512041 2.3:-2364968 MS.gene76502:CDS
ACTTAATCATCGTTCACAAA+AGG 0.517591 2.3:-2364787 MS.gene76502:CDS
CATAAAGAAGATCAAGATGA+TGG 0.521149 2.3:-2364069 MS.gene76502:CDS
GCTTAAGGAAGTGAGATCTT+CGG 0.523012 2.3:+2364532 None:intergenic
CAAACCCCTCTCTCAATACA+AGG 0.526964 2.3:-2364762 MS.gene76502:CDS
TGGGAATGGCAAGAATGAGC+TGG 0.528852 2.3:-2363917 MS.gene76502:CDS
TCTTAAGAAAGTGAGATCTC+CGG 0.549197 2.3:+2364319 None:intergenic
TCTTAAGAAAGTGAGATCTC+CGG 0.549197 2.3:+2364814 None:intergenic
CCCGTGAAGAGTCTGAAAAG+AGG 0.549764 2.3:-2364686 MS.gene76502:CDS
GAAAAGCATTGGGTGGTCAC+CGG 0.556634 2.3:-2364833 MS.gene76502:CDS
GAAAAGCATTGGGTGGTCAC+CGG 0.560535 2.3:-2364338 MS.gene76502:CDS
ACTACCTTGTATTGAGAAAG+GGG 0.563148 2.3:+2364263 None:intergenic
AAAACCCCTTTCTCAATACA+AGG 0.568454 2.3:-2364267 MS.gene76502:CDS
CCATACAAAGCATTCATGGT+TGG 0.569961 2.3:+2364150 None:intergenic
CTATGAAGAGTCAGAAAAGA+GGG 0.570873 2.3:-2364472 MS.gene76502:CDS
CCTATGAAGAGTCAGAAAAG+AGG 0.571208 2.3:-2364473 MS.gene76502:CDS
ATCTTCTTTATGATATTCAG+TGG 0.577502 2.3:+2364080 None:intergenic
TCTTTATGATATTCAGTGGG+AGG 0.578179 2.3:+2364084 None:intergenic
TGGCAGAAGAGAGATAGAAG+AGG 0.582371 2.3:-2364027 MS.gene76502:CDS
AAGCGATGAAGATGATGCTA+AGG 0.582770 2.3:-2364424 MS.gene76502:CDS
GGGAATGGCAAGAATGAGCT+GGG 0.589572 2.3:-2363916 MS.gene76502:CDS
TGAAGATGATGCTAAGGATG+AGG 0.590398 2.3:-2364418 MS.gene76502:CDS
ACTACCTTGTATTGAGAGAG+GGG 0.593179 2.3:+2364758 None:intergenic
GTGTTGGTGAAACATCAAAT+GGG 0.595282 2.3:-2363936 MS.gene76502:CDS
TAACTACCTTGTATTGAGAG+AGG 0.601425 2.3:+2364756 None:intergenic
TCTTCTTTATGATATTCAGT+GGG 0.603725 2.3:+2364081 None:intergenic
TAGTGATGAAGATGATGCTA+AGG 0.613974 2.3:-2364919 MS.gene76502:CDS
AGCAATGGAAAAGCATTGGG+TGG 0.615604 2.3:-2364840 MS.gene76502:CDS
AAGTGATGAAGATGATGCTA+AGG 0.616457 2.3:-2364637 MS.gene76502:CDS
ATGGATAAAGATCATAAGCA+TGG 0.623190 2.3:-2365002 MS.gene76502:CDS
ATGGTCACATGAGGACTCAC+CGG 0.623316 2.3:-2364131 MS.gene76502:CDS
AGCAACGGAAAAGCATTGGG+TGG 0.623901 2.3:-2364558 MS.gene76502:CDS
GGTGAAACATCAAATGGGAA+TGG 0.636070 2.3:-2363931 MS.gene76502:CDS
TGAAGATGATGCTAAGGATG+AGG 0.644459 2.3:-2364631 MS.gene76502:CDS
TGAAGATGATGCTAAGGATG+AGG 0.644459 2.3:-2364913 MS.gene76502:CDS
AGTAACGGAAAAGCATTGGG+TGG 0.649033 2.3:-2364345 MS.gene76502:CDS
GTGTATTGGTAGTGTTGAGG+CGG 0.666307 2.3:-2363998 MS.gene76502:CDS
AAAAGTTCTTGTCATAACGA+AGG 0.694153 2.3:+2363959 None:intergenic
AAAGTGTATTGGTAGTGTTG+AGG 0.694754 2.3:-2364001 MS.gene76502:CDS
ATGCTTTGTATGGTCACATG+AGG 0.725028 2.3:-2364140 MS.gene76502:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACAAGGTAGTTATAATA+GGG - chr2.3:2364085-2364104 MS.gene76502:CDS 20.0%
! AATACAAGGTAGTACTAATA+GGG - chr2.3:2364580-2364599 MS.gene76502:CDS 25.0%
! ATCTTCTTTATGATATTCAG+TGG + chr2.3:2364756-2364775 None:intergenic 25.0%
! CAATACAAGGTAGTTATAAT+AGG - chr2.3:2364084-2364103 MS.gene76502:CDS 25.0%
! TCTTCTTTATGATATTCAGT+GGG + chr2.3:2364755-2364774 None:intergenic 25.0%
! TTTGATAAAGATAGTAAGCA+TGG - chr2.3:2364113-2364132 MS.gene76502:CDS 25.0%
!! AATAAACCTTTTCCTTTCTT+TGG + chr2.3:2364971-2364990 None:intergenic 25.0%
!! TGTGGAAAAACTTTTAGTAA+CGG - chr2.3:2364473-2364492 MS.gene76502:CDS 25.0%
AAAAGTTCTTGTCATAACGA+AGG + chr2.3:2364877-2364896 None:intergenic 30.0%
ACTCATTCATCATCAACAAA+AGG - chr2.3:2364541-2364560 MS.gene76502:CDS 30.0%
ACTTAATCATCGTTCACAAA+AGG - chr2.3:2364046-2364065 MS.gene76502:CDS 30.0%
ACTTCATCATGATTCACAAA+AGG - chr2.3:2364328-2364347 MS.gene76502:CDS 30.0%
ATGGATAAAGATCATAAGCA+TGG - chr2.3:2363831-2363850 MS.gene76502:CDS 30.0%
CAATACAAGGTAGTACTAAT+AGG - chr2.3:2364579-2364598 MS.gene76502:CDS 30.0%
CATAAAGAAGATCAAGATGA+TGG - chr2.3:2364764-2364783 MS.gene76502:CDS 30.0%
GATGATGAAGAAGAAATTGA+TGG - chr2.3:2364620-2364639 MS.gene76502:CDS 30.0%
GTGATTTATCTTTGCCAAAA+TGG - chr2.3:2364786-2364805 MS.gene76502:CDS 30.0%
TACTACCTTGTATTGAGAAA+GGG + chr2.3:2364574-2364593 None:intergenic 30.0%
TGAGAAAACCTATGAAGATT+CGG - chr2.3:2363857-2363876 MS.gene76502:CDS 30.0%
! TATGACAAGAACTTTTGTGT+TGG - chr2.3:2364881-2364900 MS.gene76502:CDS 30.0%
! TGTGGAAAAACTTTTAGCAA+CGG - chr2.3:2364260-2364279 MS.gene76502:CDS 30.0%
!! GTCCTTTAAAATCTCTATTC+CGG + chr2.3:2364724-2364743 None:intergenic 30.0%
!! TTTAGCAATGGAAAAGCATT+GGG - chr2.3:2363990-2364009 MS.gene76502:CDS 30.0%
!! TTTAGTAACGGAAAAGCATT+GGG - chr2.3:2364485-2364504 MS.gene76502:CDS 30.0%
!! TTTTAGCAATGGAAAAGCAT+TGG - chr2.3:2363989-2364008 MS.gene76502:CDS 30.0%
!! TTTTAGTAACGGAAAAGCAT+TGG - chr2.3:2364484-2364503 MS.gene76502:CDS 30.0%
AAAACCCCTTTCTCAATACA+AGG - chr2.3:2364566-2364585 MS.gene76502:CDS 35.0%
AACTACCTTGTATTGAGAGA+GGG + chr2.3:2364079-2364098 None:intergenic 35.0%
AAGTGATGAAGATGATGCTA+AGG - chr2.3:2364196-2364215 MS.gene76502:CDS 35.0%
ACACATCAATGCAATGTTTG+TGG - chr2.3:2364242-2364261 MS.gene76502:CDS 35.0%
ACTACCTTGTATTGAGAAAG+GGG + chr2.3:2364573-2364592 None:intergenic 35.0%
AGTCTGAAAAGAGGATAAAG+AGG - chr2.3:2364156-2364175 MS.gene76502:CDS 35.0%
ATTCGGAAAAGAGGATGAAA+AGG - chr2.3:2363874-2363893 MS.gene76502:CDS 35.0%
CATGATGAAGTTTCTGCTTA+AGG + chr2.3:2364319-2364338 None:intergenic 35.0%
CTATGAAGAGTCAGAAAAGA+GGG - chr2.3:2364361-2364380 MS.gene76502:CDS 35.0%
GAATGAGTCGTATGTTATAG+AGG - chr2.3:2364997-2365016 MS.gene76502:CDS 35.0%
GTACTACCTTGTATTGAGAA+AGG + chr2.3:2364575-2364594 None:intergenic 35.0%
TAACTACCTTGTATTGAGAG+AGG + chr2.3:2364080-2364099 None:intergenic 35.0%
TAGTGATGAAGATGATGCTA+AGG - chr2.3:2363914-2363933 MS.gene76502:CDS 35.0%
TCTTAAGAAAGTGAGATCTC+CGG + chr2.3:2364022-2364041 None:intergenic 35.0%
TCTTTATGATATTCAGTGGG+AGG + chr2.3:2364752-2364771 None:intergenic 35.0%
! AGAAGAGGTAGAAAGTGTAT+TGG - chr2.3:2364821-2364840 MS.gene76502:CDS 35.0%
! CACCGGAATAGAGATTTTAA+AGG - chr2.3:2364719-2364738 MS.gene76502:CDS 35.0%
! GTGTTGGTGAAACATCAAAT+GGG - chr2.3:2364897-2364916 MS.gene76502:CDS 35.0%
! TGTGGGAAAACTTTTAGCAA+TGG - chr2.3:2363978-2363997 MS.gene76502:CDS 35.0%
! TGTGTTGGTGAAACATCAAA+TGG - chr2.3:2364896-2364915 MS.gene76502:CDS 35.0%
! TTTTTGCAGAGAGAACAAGT+TGG + chr2.3:2364656-2364675 None:intergenic 35.0%
!! AAAGTGTATTGGTAGTGTTG+AGG - chr2.3:2364832-2364851 MS.gene76502:CDS 35.0%
!! TTTAGCAACGGAAAAGCATT+GGG - chr2.3:2364272-2364291 MS.gene76502:CDS 35.0%
!! TTTTAGCAACGGAAAAGCAT+TGG - chr2.3:2364271-2364290 MS.gene76502:CDS 35.0%
AAGCAGCCAAAGAAAGGAAA+AGG - chr2.3:2364962-2364981 MS.gene76502:CDS 40.0%
AAGCGATGAAGATGATGCTA+AGG - chr2.3:2364409-2364428 MS.gene76502:CDS 40.0%
ACACATCAGTGCAATGTTTG+TGG - chr2.3:2364455-2364474 MS.gene76502:CDS 40.0%
ACTACCTTGTATTGAGAGAG+GGG + chr2.3:2364078-2364097 None:intergenic 40.0%
AGTCAGAAAAGAGGGTGAAA+AGG - chr2.3:2364369-2364388 MS.gene76502:CDS 40.0%
AGTGATAAGCAGCCAAAGAA+AGG - chr2.3:2364956-2364975 MS.gene76502:CDS 40.0%
ATGCTTTGTATGGTCACATG+AGG - chr2.3:2364693-2364712 MS.gene76502:CDS 40.0%
CACATCAGTGCAATGTTTGT+GGG - chr2.3:2363961-2363980 MS.gene76502:CDS 40.0%
CCAACCATGAATGCTTTGTA+TGG - chr2.3:2364683-2364702 MS.gene76502:CDS 40.0%
CCTATGAAGAGTCAGAAAAG+AGG - chr2.3:2364360-2364379 MS.gene76502:CDS 40.0%
CCTATGAAGATTCGGAAAAG+AGG - chr2.3:2363865-2363884 MS.gene76502:CDS 40.0%
GCTTAAGGAAGTGAGATCTT+CGG + chr2.3:2364304-2364323 None:intergenic 40.0%
GGTGAAACATCAAATGGGAA+TGG - chr2.3:2364902-2364921 MS.gene76502:CDS 40.0%
TCACATCAGTGCAATGTTTG+TGG - chr2.3:2363960-2363979 MS.gene76502:CDS 40.0%
TGAAGATGATGCTAAGGATG+AGG - chr2.3:2363920-2363939 MS.gene76502:CDS 40.0%
! CCATACAAAGCATTCATGGT+TGG + chr2.3:2364686-2364705 None:intergenic 40.0%
! CCTCTTTTCCGAATCTTCAT+AGG + chr2.3:2363868-2363887 None:intergenic 40.0%
! CCTCTTTTCTGACTCTTCAT+AGG + chr2.3:2364363-2364382 None:intergenic 40.0%
! GTGACCATACAAAGCATTCA+TGG + chr2.3:2364690-2364709 None:intergenic 40.0%
! TCCTCTTTTCAGACTCTTCA+CGG + chr2.3:2364151-2364170 None:intergenic 40.0%
!! CTATCTCTCTTCTGCCATTT+TGG + chr2.3:2364803-2364822 None:intergenic 40.0%
CAAACCCCTCTCTCAATACA+AGG - chr2.3:2364071-2364090 MS.gene76502:CDS 45.0%
TGGCAGAAGAGAGATAGAAG+AGG - chr2.3:2364806-2364825 MS.gene76502:CDS 45.0%
! CCTCTTTTCAGACTCTTCAC+GGG + chr2.3:2364150-2364169 None:intergenic 45.0%
!! AGCAATGGAAAAGCATTGGG+TGG - chr2.3:2363993-2364012 MS.gene76502:CDS 45.0%
!! AGTAACGGAAAAGCATTGGG+TGG - chr2.3:2364488-2364507 MS.gene76502:CDS 45.0%
!! GTGTATTGGTAGTGTTGAGG+CGG - chr2.3:2364835-2364854 MS.gene76502:CDS 45.0%
ATGGTCACATGAGGACTCAC+CGG - chr2.3:2364702-2364721 MS.gene76502:CDS 50.0%
CCCGTGAAGAGTCTGAAAAG+AGG - chr2.3:2364147-2364166 MS.gene76502:CDS 50.0%
GGGAATGGCAAGAATGAGCT+GGG - chr2.3:2364917-2364936 MS.gene76502:CDS 50.0%
TGGGAATGGCAAGAATGAGC+TGG - chr2.3:2364916-2364935 MS.gene76502:CDS 50.0%
!! AGCAACGGAAAAGCATTGGG+TGG - chr2.3:2364275-2364294 MS.gene76502:CDS 50.0%
!! GAAAAGCATTGGGTGGTCAC+CGG - chr2.3:2364000-2364019 MS.gene76502:CDS 50.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 2363831 2365024 2363831 ID=MS.gene76502
chr2.3 mRNA 2363831 2365024 2363831 ID=MS.gene76502.t1;Parent=MS.gene76502
chr2.3 exon 2363831 2365024 2363831 ID=MS.gene76502.t1.exon1;Parent=MS.gene76502.t1
chr2.3 CDS 2363831 2365024 2363831 ID=cds.MS.gene76502.t1;Parent=MS.gene76502.t1
Gene Sequence

>MS.gene76502

ATGGATAAAGATCATAAGCATGGTTATGAGAAAACCTATGAAGATTCGGAAAAGAGGATGAAAAGGTCTTCATGTTCACTATCTAGTGATGAAGATGATGCTAAGGATGAGGTTTCTGTTCAAGAATCTTCACATCAGTGCAATGTTTGTGGGAAAACTTTTAGCAATGGAAAAGCATTGGGTGGTCACCGGAGATCTCACTTTCTTAAGAAGAAACTTAATCATCGTTCACAAAAGGTACAAACCCCTCTCTCAATACAAGGTAGTTATAATAGGGCTAGTTTTGATAAAGATAGTAAGCATGGTTTTGAGAACACCCGTGAAGAGTCTGAAAAGAGGATAAAGAGGTCTTTTAGTTCTCTATCAAGTGATGAAGATGATGCTAAGGATGAGGTTTCTATTCAAGAATCTACACATCAATGCAATGTTTGTGGAAAAACTTTTAGCAACGGAAAAGCATTGGGTGGTCACCGAAGATCTCACTTCCTTAAGCAGAAACTTCATCATGATTCACAAAAGGTTGATAATACCTATGAAGAGTCAGAAAAGAGGGTGAAAAGGTCAGCTTGTTCTCTATCAAGCGATGAAGATGATGCTAAGGATGAGGTTTCTGTTCAAGAATCTACACATCAGTGCAATGTTTGTGGAAAAACTTTTAGTAACGGAAAAGCATTGGGTGGTCACCGGAGATCTCACTTTCTTAAGAAGAAACTCATTCATCATCAACAAAAGGTGAAAACCCCTTTCTCAATACAAGGTAGTACTAATAGGGCTAGTTTTGATGATTATGATGATGAAGAAGAAATTGATGGCATAGAGAAACCAACTTGTTCTCTCTGCAAAAAGAAGTTTCCAACCATGAATGCTTTGTATGGTCACATGAGGACTCACCGGAATAGAGATTTTAAAGGACTTAATCCTCCCACTGAATATCATAAAGAAGATCAAGATGATGGTGATTTATCTTTGCCAAAATGGCAGAAGAGAGATAGAAGAGGTAGAAAGTGTATTGGTAGTGTTGAGGCGGCTGCAAATCTTTTGCACCTTCGTTATGACAAGAACTTTTGTGTTGGTGAAACATCAAATGGGAATGGCAAGAATGAGCTGGGCAGTGATAGAAACAGCAGTGATAAGCAGCCAAAGAAAGGAAAAGGTTTATTTGATTTGAATGAGTCGTATGTTATAGAGGACTAA
Protein sequence

>MS.gene76502.t1

MDKDHKHGYEKTYEDSEKRMKRSSCSLSSDEDDAKDEVSVQESSHQCNVCGKTFSNGKALGGHRRSHFLKKKLNHRSQKVQTPLSIQGSYNRASFDKDSKHGFENTREESEKRIKRSFSSLSSDEDDAKDEVSIQESTHQCNVCGKTFSNGKALGGHRRSHFLKQKLHHDSQKVDNTYEESEKRVKRSACSLSSDEDDAKDEVSVQESTHQCNVCGKTFSNGKALGGHRRSHFLKKKLIHHQQKVKTPFSIQGSTNRASFDDYDDEEEIDGIEKPTCSLCKKKFPTMNALYGHMRTHRNRDFKGLNPPTEYHKEDQDDGDLSLPKWQKRDRRGRKCIGSVEAAANLLHLRYDKNFCVGETSNGNGKNELGSDRNSSDKQPKKGKGLFDLNESYVIED