AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76584

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76584.t1 MTR_5g040740 78.836 378 29 4 25 399 81 410 0.0 571
MS.gene76584.t1 MTR_5g040880 60.820 439 101 8 25 395 80 515 7.61e-168 480
MS.gene76584.t1 MTR_5g082140 60.000 385 127 7 25 389 81 458 8.50e-146 422
MS.gene76584.t1 MTR_2g024430 64.157 332 113 5 25 354 90 417 6.36e-138 404
MS.gene76584.t1 MTR_5g061220 47.333 450 146 13 26 395 89 527 5.05e-111 335
MS.gene76584.t1 MTR_2g006270 46.193 394 160 11 25 393 84 450 1.20e-98 301
MS.gene76584.t1 MTR_2g006380 42.092 392 170 12 26 393 92 450 8.08e-86 268
MS.gene76584.t1 MTR_1g058210 45.119 379 173 16 26 387 82 442 6.56e-83 261
MS.gene76584.t1 MTR_3g073420 42.328 378 162 11 25 354 86 455 8.28e-83 265
MS.gene76584.t1 MTR_2g094570 37.437 398 178 12 26 363 78 464 3.84e-68 228
MS.gene76584.t1 MTR_2g094570 37.437 398 178 12 26 363 78 464 8.02e-68 228
MS.gene76584.t1 MTR_2g094570 37.437 398 178 12 26 363 134 520 1.46e-67 228
MS.gene76584.t1 MTR_1g064430 39.943 348 143 8 54 344 127 465 6.72e-66 222
MS.gene76584.t1 MTR_1g064430 39.943 348 143 8 54 344 140 478 7.63e-66 222
MS.gene76584.t1 MTR_4g058930 39.503 362 138 11 54 349 107 453 4.03e-65 220
MS.gene76584.t1 MTR_4g058930 39.503 362 138 11 54 349 107 453 8.75e-65 219
MS.gene76584.t1 MTR_1g094960 37.922 385 167 13 25 347 84 458 2.99e-64 216
MS.gene76584.t1 MTR_5g060630 40.000 400 159 15 26 350 82 475 4.19e-63 211
MS.gene76584.t1 MTR_5g460740 40.000 400 159 15 26 350 82 475 4.19e-63 211
MS.gene76584.t1 MTR_5g060780 44.521 292 132 11 26 301 82 359 6.88e-61 202
MS.gene76584.t1 MTR_5g460920 44.521 292 132 11 26 301 82 359 3.72e-60 200
MS.gene76584.t1 MTR_5g061890 43.810 315 136 13 26 324 82 371 2.39e-57 193
MS.gene76584.t1 MTR_7g101280 35.196 358 161 11 55 350 130 478 5.05e-53 187
MS.gene76584.t1 MTR_0041s0110 35.196 358 161 11 55 350 130 478 5.05e-53 187
MS.gene76584.t1 MTR_7g101275 41.364 220 111 5 55 260 91 306 1.87e-49 172
MS.gene76584.t1 MTR_0041s0120 38.261 230 116 5 55 260 91 318 5.07e-43 155
MS.gene76584.t1 MTR_2g014770 35.470 234 130 6 58 276 160 387 3.34e-42 156
MS.gene76584.t1 MTR_2g014770 35.470 234 130 6 58 276 160 387 5.40e-42 157
MS.gene76584.t1 MTR_4g088210 35.897 234 128 7 58 276 150 376 1.61e-41 155
MS.gene76584.t1 MTR_4g088210 35.897 234 128 7 58 276 150 376 1.62e-41 155
MS.gene76584.t1 MTR_2g093740 36.014 286 156 10 58 324 40 317 4.05e-41 154
MS.gene76584.t1 MTR_2g093740 36.014 286 156 10 58 324 40 317 5.24e-41 154
MS.gene76584.t1 MTR_2g093740 36.014 286 156 10 58 324 170 447 6.24e-41 154
MS.gene76584.t1 MTR_5g060770 36.873 339 148 15 26 350 28 314 6.57e-41 148
MS.gene76584.t1 MTR_2g093740 36.014 286 156 10 58 324 170 447 9.51e-41 154
MS.gene76584.t1 MTR_2g093740 36.014 286 156 10 58 324 170 447 1.15e-40 154
MS.gene76584.t1 MTR_4g060460 37.647 255 136 9 58 295 168 416 7.93e-38 145
MS.gene76584.t1 MTR_2g093740 34.448 299 156 11 58 324 40 330 8.21e-38 144
MS.gene76584.t1 MTR_4g021580 32.526 289 170 8 25 295 90 371 8.59e-38 144
MS.gene76584.t1 MTR_4g021580 32.404 287 171 7 26 295 91 371 8.68e-38 145
MS.gene76584.t1 MTR_4g060460 37.647 255 136 9 58 295 168 416 8.91e-38 145
MS.gene76584.t1 MTR_2g093740 34.448 299 156 11 58 324 40 330 1.20e-37 144
MS.gene76584.t1 MTR_2g093740 34.448 299 156 11 58 324 170 460 1.51e-37 144
MS.gene76584.t1 MTR_2g005240 35.987 314 165 13 1 295 78 374 1.75e-37 144
MS.gene76584.t1 MTR_2g093740 34.448 299 156 11 58 324 170 460 1.79e-37 144
MS.gene76584.t1 MTR_2g093740 34.448 299 156 11 58 324 170 460 2.14e-37 144
MS.gene76584.t1 MTR_2g018690 33.977 259 141 9 58 298 129 375 4.80e-37 143
MS.gene76584.t1 MTR_8g101360 34.874 238 133 7 58 279 137 368 1.60e-36 142
MS.gene76584.t1 MTR_8g079492 34.241 257 143 9 58 298 46 292 5.27e-36 140
MS.gene76584.t1 MTR_8g079492 34.241 257 143 9 58 298 128 374 6.14e-36 140
MS.gene76584.t1 MTR_8g079492 34.241 257 143 9 58 298 128 374 6.37e-36 140
MS.gene76584.t1 MTR_5g062970 53.226 124 55 1 26 146 105 228 1.27e-35 131
MS.gene76584.t1 MTR_5g076270 34.241 257 143 9 58 298 130 376 1.46e-35 139
MS.gene76584.t1 MTR_8g100050 33.591 259 150 7 53 295 159 411 2.28e-35 138
MS.gene76584.t1 MTR_7g062540 30.729 384 227 17 1 364 69 433 2.74e-35 137
MS.gene76584.t1 MTR_8g100050 33.591 259 150 7 53 295 159 411 3.26e-35 138
MS.gene76584.t1 MTR_3g064050 33.852 257 144 9 58 298 132 378 7.59e-35 137
MS.gene76584.t1 MTR_1g024025 35.021 237 128 8 58 276 148 376 4.23e-34 135
MS.gene76584.t1 MTR_2g043250 32.203 236 139 6 58 278 128 357 7.38e-33 131
MS.gene76584.t1 MTR_8g027440 36.170 235 125 9 58 276 133 358 7.46e-33 130
MS.gene76584.t1 MTR_4g124900 31.727 249 148 7 58 290 128 370 9.01e-33 131
MS.gene76584.t1 MTR_4g124900 31.727 249 148 7 58 290 128 370 1.02e-32 130
MS.gene76584.t1 MTR_5g062960 40.212 189 78 6 174 354 2 163 1.11e-29 114
MS.gene76584.t1 MTR_8g446900 28.720 289 175 9 52 318 58 337 2.27e-26 109
MS.gene76584.t1 MTR_5g460910 31.268 339 145 15 26 350 45 309 6.62e-22 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76584.t1 AT1G77850 36.709 395 192 11 9 349 61 451 2.18e-72 237
MS.gene76584.t1 AT1G77850 36.524 397 190 11 9 349 61 451 2.76e-72 237
MS.gene76584.t1 AT4G30080 39.628 376 164 10 25 345 84 451 2.31e-67 226
MS.gene76584.t1 AT2G28350 42.617 298 112 9 54 298 113 404 6.93e-62 211
MS.gene76584.t1 AT2G28350 41.346 312 124 9 40 298 99 404 1.11e-61 211
MS.gene76584.t1 AT2G33860 37.607 234 125 7 58 276 4 231 2.01e-42 154
MS.gene76584.t1 AT2G33860 37.607 234 125 7 58 276 161 388 1.90e-41 154
MS.gene76584.t1 AT1G35520 36.177 293 152 11 18 295 100 372 1.77e-39 149
MS.gene76584.t1 AT5G60450 38.528 231 126 7 58 276 155 381 8.64e-39 148
MS.gene76584.t1 AT5G60450 38.528 231 126 7 58 276 179 405 8.84e-39 148
MS.gene76584.t1 AT4G23980 32.979 282 156 12 15 280 82 346 2.86e-37 143
MS.gene76584.t1 AT4G23980 32.979 282 156 12 15 280 82 346 3.08e-37 143
MS.gene76584.t1 AT5G37020 32.941 255 149 8 58 298 33 279 6.24e-37 142
MS.gene76584.t1 AT5G37020 32.941 255 149 8 58 298 128 374 7.33e-37 142
MS.gene76584.t1 AT5G37020 32.941 255 149 8 58 298 128 374 7.59e-37 142
MS.gene76584.t1 AT1G34390 34.014 294 155 12 18 295 100 370 3.16e-36 140
MS.gene76584.t1 AT1G34410 36.680 259 139 10 53 295 123 372 3.66e-36 139
MS.gene76584.t1 AT1G19850 36.170 235 128 8 58 276 149 377 1.56e-35 139
MS.gene76584.t1 AT3G61830 36.910 233 125 7 58 276 79 303 1.68e-35 137
MS.gene76584.t1 AT1G19850 36.170 235 128 8 58 276 160 388 1.71e-35 139
MS.gene76584.t1 AT2G46530 37.131 237 125 9 58 279 39 266 1.74e-35 137
MS.gene76584.t1 AT3G61830 36.910 233 125 7 58 276 130 354 2.74e-35 137
MS.gene76584.t1 AT1G30330 33.074 257 146 8 58 298 131 377 4.23e-35 137
MS.gene76584.t1 AT1G30330 33.074 257 146 8 58 298 131 377 4.23e-35 137
MS.gene76584.t1 AT1G30330 33.074 257 146 8 58 298 129 375 4.60e-35 137
MS.gene76584.t1 AT2G46530 37.131 237 125 9 58 279 126 353 4.73e-35 136
MS.gene76584.t1 AT1G35240 35.521 259 142 9 53 295 123 372 6.18e-35 136
MS.gene76584.t1 AT2G46530 37.131 237 125 9 58 279 147 374 7.21e-35 136
MS.gene76584.t1 AT1G34310 33.667 300 150 12 18 295 100 372 7.86e-35 135
MS.gene76584.t1 AT1G59750 33.566 286 167 9 25 293 93 372 1.06e-34 136
MS.gene76584.t1 AT1G59750 33.566 286 167 9 25 293 93 372 1.19e-34 135
MS.gene76584.t1 AT1G59750 33.566 286 167 9 25 293 93 372 1.44e-34 135
MS.gene76584.t1 AT1G59750 33.566 286 167 9 25 293 93 372 1.44e-34 135
MS.gene76584.t1 AT5G62000 34.363 259 148 8 53 295 161 413 2.22e-34 135
MS.gene76584.t1 AT5G62000 34.363 259 148 8 53 295 161 413 2.22e-34 135
MS.gene76584.t1 AT5G62000 34.363 259 148 8 53 295 161 413 2.22e-34 135
MS.gene76584.t1 AT5G62000 34.363 259 148 8 53 295 161 413 2.22e-34 135
MS.gene76584.t1 AT5G62000 34.363 259 148 8 53 295 161 413 2.89e-34 135
MS.gene76584.t1 AT1G35540 34.296 277 140 11 18 276 100 352 5.74e-34 133
MS.gene76584.t1 AT1G19220 33.071 254 148 8 58 295 128 375 4.79e-33 132
MS.gene76584.t1 AT5G20730 31.873 251 150 6 58 292 129 374 1.62e-32 130
MS.gene76584.t1 AT5G20730 31.873 251 150 6 58 292 129 374 1.84e-32 130
MS.gene76584.t1 AT5G20730 32.271 251 148 7 58 292 129 373 5.30e-32 129
MS.gene76584.t1 AT1G34170 33.333 237 130 10 58 276 126 352 2.57e-28 116
MS.gene76584.t1 AT1G34170 33.040 227 118 10 58 276 126 326 3.19e-23 101
MS.gene76584.t1 AT1G34170 33.040 227 118 10 58 276 126 326 4.55e-23 101
MS.gene76584.t1 AT1G43950 38.971 136 73 4 23 157 96 222 6.92e-18 82.4

Find 94 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCAATATCATAGGTTAAT+TGG 0.114277 5.1:+30921708 MS.gene76584:intron
AGGATTCCATTTAATCTTAA+TGG 0.197798 5.1:-30921055 None:intergenic
CCTGGTTCAATTATGTTATT+TGG 0.201738 5.1:+30922043 MS.gene76584:CDS
GAAGAATGAAATTCTATCTT+TGG 0.239725 5.1:-30921896 None:intergenic
CGAACCAGCTGCGAAACTTT+AGG 0.296884 5.1:-30921737 None:intergenic
AGGAAGCCATTAAGATTAAA+TGG 0.321373 5.1:+30921049 MS.gene76584:CDS
GGAATTGGCCGATAAGAAAA+TGG 0.351667 5.1:+30920957 MS.gene76584:CDS
GGTCGTTAGTGACGGGAGTT+AGG 0.352092 5.1:-30920513 None:intergenic
TGTTACTGAGGCCGTGGAAT+TGG 0.365443 5.1:+30920942 MS.gene76584:CDS
GTCAATATCATAGGTTAATT+GGG 0.372441 5.1:+30921709 MS.gene76584:intron
CACTCCAGTTGGTGGTGATC+AGG 0.388147 5.1:-30920786 None:intergenic
ATTCAACGATGTTTCATTGA+AGG 0.410291 5.1:-30921930 None:intergenic
TTCGAGTGCGAGTCAGTCCT+TGG 0.414064 5.1:+30921756 MS.gene76584:CDS
GGGGTTGACGTTGTTTCCTC+TGG 0.416086 5.1:+30920577 MS.gene76584:CDS
GGAATCCGTCGGCAGAAGTT+TGG 0.417648 5.1:+30920892 MS.gene76584:CDS
AGCATGCGCCATGGACGATT+AGG 0.434996 5.1:-30921165 None:intergenic
TGCTTTGCTCGATGACATGC+AGG 0.446126 5.1:+30921958 MS.gene76584:CDS
TGCCTTACACAATGATATAG+AGG 0.453622 5.1:+30921859 MS.gene76584:CDS
GAATGTTTAATTCTCAATCC+AGG 0.457999 5.1:-30921075 None:intergenic
TTCGTGAAGACGAAGATGAC+GGG 0.459706 5.1:+30920557 MS.gene76584:CDS
TGTAGTTAATGCTAAGGTTG+TGG 0.462109 5.1:+30921026 MS.gene76584:CDS
ATTAAGATTAAATGGAATCC+TGG 0.463670 5.1:+30921057 MS.gene76584:CDS
ATGAAATTCTATCTTTGGAA+TGG 0.465057 5.1:-30921891 None:intergenic
ATTCCCGCCACTTGACCTCC+AGG 0.472125 5.1:+30920672 MS.gene76584:CDS
TTTACACGATTCAGATATCA+AGG 0.473052 5.1:+30922093 MS.gene76584:CDS
TCGATGACATGCAGGGAGCC+AGG 0.473164 5.1:+30921966 MS.gene76584:CDS
CTGACATTCACGGCAAAGAA+TGG 0.477327 5.1:+30920725 MS.gene76584:CDS
ATCAACCGCGGAGACGGTGC+AGG 0.485860 5.1:-30920451 None:intergenic
CCAGGCCGAAAAGCCGTCTC+AGG 0.490565 5.1:+30920690 MS.gene76584:CDS
GAGACGGTGCAGGGAATGAA+TGG 0.491412 5.1:-30920441 None:intergenic
TCTTCGTCTTCACGAACCTC+AGG 0.495857 5.1:-30920550 None:intergenic
CGGCCTGGAGGTCAAGTGGC+GGG 0.508553 5.1:-30920675 None:intergenic
TCGTGTAAATCACTTCCAAC+AGG 0.511200 5.1:-30922079 None:intergenic
TCCCTCTATATCATTGTGTA+AGG 0.511256 5.1:-30921861 None:intergenic
GACACCTGATCACCACCAAC+TGG 0.514844 5.1:+30920782 MS.gene76584:CDS
TGAACCTAAAGTTTCGCAGC+TGG 0.518797 5.1:+30921733 MS.gene76584:CDS
AAAATCTGTTACTGAGGCCG+TGG 0.525649 5.1:+30920936 MS.gene76584:CDS
CTCTCAGTCACTGACATTCA+CGG 0.532201 5.1:+30920715 MS.gene76584:CDS
CCTCAGGAGGTTCGTGGACA+CGG 0.532546 5.1:-30920534 None:intergenic
CACGAACCTCAGGAGGTTCG+TGG 0.537961 5.1:-30920540 None:intergenic
TCGAGTGCGAGTCAGTCCTT+GGG 0.537997 5.1:+30921757 MS.gene76584:CDS
TTTCTTATCGGCCAATTCCA+CGG 0.546106 5.1:-30920953 None:intergenic
GTTGACGAGAAGAAACTGAT+TGG 0.549713 5.1:+30920814 MS.gene76584:CDS
GAGTAGATCAACCGCGGAGA+CGG 0.550031 5.1:-30920457 None:intergenic
GTATATTATCCTACTGCTGA+AGG 0.552234 5.1:+30920991 MS.gene76584:CDS
TCGTCCATGGCGCATGCTTG+AGG 0.553392 5.1:+30921170 MS.gene76584:CDS
TCGGCCTGGAGGTCAAGTGG+CGG 0.557087 5.1:-30920676 None:intergenic
GGTGGTGATCAGGTGTCGCA+AGG 0.563669 5.1:-30920776 None:intergenic
GGAACTGCAATAATCTCCGT+TGG 0.563886 5.1:+30920871 MS.gene76584:CDS
AGCTTCGCCACGTCTACCGT+GGG 0.564056 5.1:+30920749 MS.gene76584:CDS
GTGGACACGGTCGTTAGTGA+CGG 0.566445 5.1:-30920521 None:intergenic
CGGTGCAGGGAATGAATGGA+CGG 0.567424 5.1:-30920437 None:intergenic
GAATGAATGGACGGGAACCA+TGG 0.573275 5.1:-30920428 None:intergenic
ACTCCGTCTGATGCTAATAA+CGG 0.577996 5.1:+30920610 MS.gene76584:CDS
TCACACCAACCTTCAGCAGT+AGG 0.580736 5.1:-30921000 None:intergenic
TATCTGCTCCTAATCGTCCA+TGG 0.582308 5.1:+30921157 MS.gene76584:CDS
AACAAACTCACTCCAGTTGG+TGG 0.582643 5.1:-30920794 None:intergenic
AAATTCTATCTTTGGAATGG+AGG 0.586110 5.1:-30921888 None:intergenic
GAAATAAGCTCAACCTCCCA+AGG 0.586576 5.1:-30921773 None:intergenic
GGTCAGATCATACGTCCTGT+TGG 0.588139 5.1:+30922064 MS.gene76584:CDS
TGGACACGGTCGTTAGTGAC+GGG 0.588676 5.1:-30920520 None:intergenic
TCGTCTTCACGAACCTCAGG+AGG 0.589597 5.1:-30920547 None:intergenic
CCAAATAACATAATTGAACC+AGG 0.590972 5.1:-30922043 None:intergenic
TGGATGTAGTGCATGTATGT+TGG 0.599102 5.1:-30921795 None:intergenic
TACACGATTCAGATATCAAG+GGG 0.600673 5.1:+30922095 MS.gene76584:CDS
AGGAGTAGTTTGACGTTCGT+CGG 0.604774 5.1:-30920493 None:intergenic
GTTCGTGAAGACGAAGATGA+CGG 0.606081 5.1:+30920556 MS.gene76584:CDS
ATTATCCTACTGCTGAAGGT+TGG 0.606451 5.1:+30920995 MS.gene76584:CDS
GCCTTACACAATGATATAGA+GGG 0.612818 5.1:+30921860 MS.gene76584:CDS
AGTTAATGCTAAGGTTGTGG+AGG 0.615178 5.1:+30921029 MS.gene76584:CDS
AGGGAGTCCCACGGTAGACG+TGG 0.627277 5.1:-30920756 None:intergenic
GTGGTGATCAGGTGTCGCAA+GGG 0.628071 5.1:-30920775 None:intergenic
TAATCTCCGTTGGAATCCGT+CGG 0.630455 5.1:+30920881 MS.gene76584:CDS
CAGCACCAAACTTCTGCCGA+CGG 0.631188 5.1:-30920897 None:intergenic
TATACCTCAAGCATGCGCCA+TGG 0.631418 5.1:-30921174 None:intergenic
GGTGCAGGGAATGAATGGAC+GGG 0.631616 5.1:-30920436 None:intergenic
AGTGCGAGTCAGTCCTTGGG+AGG 0.635441 5.1:+30921760 MS.gene76584:CDS
TTACACGATTCAGATATCAA+GGG 0.635502 5.1:+30922094 MS.gene76584:CDS
GTCAACAAACTCACTCCAGT+TGG 0.636827 5.1:-30920797 None:intergenic
TCAACCGCGGAGACGGTGCA+GGG 0.637633 5.1:-30920450 None:intergenic
AAGCTTCGCCACGTCTACCG+TGG 0.643601 5.1:+30920748 MS.gene76584:CDS
CCGTGTCCACGAACCTCCTG+AGG 0.646741 5.1:+30920534 MS.gene76584:CDS
GACGAGAAGAAACTGATTGG+TGG 0.658536 5.1:+30920817 MS.gene76584:CDS
GCTTTGCTCGATGACATGCA+GGG 0.669755 5.1:+30921959 MS.gene76584:CDS
CCACCGTTATTAGCATCAGA+CGG 0.670947 5.1:-30920613 None:intergenic
GTCAGCGAGTAGATCAACCG+CGG 0.673456 5.1:-30920463 None:intergenic
GTGACTGAGAGTTCCTGAGA+CGG 0.676102 5.1:-30920703 None:intergenic
CATTCCCTGCACCGTCTCCG+CGG 0.679524 5.1:+30920446 MS.gene76584:CDS
GGTGTCGCAAGGGAGTCCCA+CGG 0.699974 5.1:-30920765 None:intergenic
TCGTGAAGACGAAGATGACG+GGG 0.700053 5.1:+30920558 MS.gene76584:CDS
GCAGTTGGAAGAAGTTGATG+CGG 0.705608 5.1:-30922002 None:intergenic
CCGTCTGATGCTAATAACGG+TGG 0.711385 5.1:+30920613 MS.gene76584:CDS
CTTCTGCCGACGGATTCCAA+CGG 0.718297 5.1:-30920887 None:intergenic
ACACGATTCAGATATCAAGG+GGG 0.776881 5.1:+30922096 MS.gene76584:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTTTAATAAATGTTAT+CGG + chr5.1:30921262-30921281 MS.gene76584:intron 10.0%
!!! TTTTTTTTTTTTTTAGCAAA+TGG + chr5.1:30921519-30921538 MS.gene76584:intron 10.0%
!!! ACATCTCATTTTATTTTTTA+AGG - chr5.1:30921653-30921672 None:intergenic 15.0%
!! AGAAACTTTATACTTGAAAT+AGG + chr5.1:30921483-30921502 MS.gene76584:intron 20.0%
!! ATTATAGTTAATTCATGATG+TGG - chr5.1:30921457-30921476 None:intergenic 20.0%
!! ATTTGTACAATATACTTTGT+TGG + chr5.1:30921578-30921597 MS.gene76584:intron 20.0%
!! CAAAATTCGCTATATTTATA+GGG - chr5.1:30921622-30921641 None:intergenic 20.0%
!! TCAAAATTCGCTATATTTAT+AGG - chr5.1:30921623-30921642 None:intergenic 20.0%
!!! AATGTTTAAGATTAACACAT+TGG - chr5.1:30921380-30921399 None:intergenic 20.0%
!!! ATATCCTATATTTTTCACAT+TGG - chr5.1:30921354-30921373 None:intergenic 20.0%
!!! ATTTTTCATGTCAATATCAT+AGG + chr5.1:30921700-30921719 MS.gene76584:intron 20.0%
!!! GATGTTTTAAATGATTGATA+CGG + chr5.1:30921668-30921687 MS.gene76584:intron 20.0%
!!! TGATTTTTTCATGAAAACTA+AGG - chr5.1:30920848-30920867 None:intergenic 20.0%
! AGGATTCCATTTAATCTTAA+TGG - chr5.1:30921058-30921077 None:intergenic 25.0%
! ATTAAGATTAAATGGAATCC+TGG + chr5.1:30921057-30921076 MS.gene76584:CDS 25.0%
! GAAGAATGAAATTCTATCTT+TGG - chr5.1:30921899-30921918 None:intergenic 25.0%
! GTCAATATCATAGGTTAATT+GGG + chr5.1:30921709-30921728 MS.gene76584:intron 25.0%
! GTTGTGCAATTAAATTTAAG+TGG + chr5.1:30921403-30921422 MS.gene76584:intron 25.0%
! TGTCAATATCATAGGTTAAT+TGG + chr5.1:30921708-30921727 MS.gene76584:intron 25.0%
! TGTTCCAATGTGAAAAATAT+AGG + chr5.1:30921347-30921366 MS.gene76584:intron 25.0%
!! ATGAAATTCTATCTTTGGAA+TGG - chr5.1:30921894-30921913 None:intergenic 25.0%
!! GTTTTCATGAAAAAATCAAC+TGG + chr5.1:30920850-30920869 MS.gene76584:CDS 25.0%
!! TGATTTTGTAGTTAATGCTA+AGG + chr5.1:30921020-30921039 MS.gene76584:CDS 25.0%
!!! TTCAAAAGCCATTTTCTTAT+CGG - chr5.1:30920968-30920987 None:intergenic 25.0%
!!! TTTTTTGATCAGTTCATGTT+AGG + chr5.1:30921317-30921336 MS.gene76584:intron 25.0%
AACAGAAAAATCTGTTACTG+AGG + chr5.1:30920930-30920949 MS.gene76584:CDS 30.0%
AATGTTATCGGTCAAAGTTA+TGG + chr5.1:30921274-30921293 MS.gene76584:intron 30.0%
AGGAAGCCATTAAGATTAAA+TGG + chr5.1:30921049-30921068 MS.gene76584:CDS 30.0%
ATTCAACGATGTTTCATTGA+AGG - chr5.1:30921933-30921952 None:intergenic 30.0%
CCAAATAACATAATTGAACC+AGG - chr5.1:30922046-30922065 None:intergenic 30.0%
CCTGGTTCAATTATGTTATT+TGG + chr5.1:30922043-30922062 MS.gene76584:CDS 30.0%
GAATGTTTAATTCTCAATCC+AGG - chr5.1:30921078-30921097 None:intergenic 30.0%
TCAAAGTTATGGTTCATGTT+AGG + chr5.1:30921285-30921304 MS.gene76584:intron 30.0%
TTACACGATTCAGATATCAA+GGG + chr5.1:30922094-30922113 MS.gene76584:CDS 30.0%
TTTACACGATTCAGATATCA+AGG + chr5.1:30922093-30922112 MS.gene76584:CDS 30.0%
! AAAGAGATGCTCAAATTTTG+AGG + chr5.1:30921119-30921138 MS.gene76584:CDS 30.0%
! AAGAGATGCTCAAATTTTGA+GGG + chr5.1:30921120-30921139 MS.gene76584:CDS 30.0%
! TATTTTCATTGCATCCTCTA+CGG - chr5.1:30922129-30922148 None:intergenic 30.0%
!! AAATTCTATCTTTGGAATGG+AGG - chr5.1:30921891-30921910 None:intergenic 30.0%
AACACCAAAAAACTTAGTCC+TGG + chr5.1:30922025-30922044 MS.gene76584:CDS 35.0%
GCCTTACACAATGATATAGA+GGG + chr5.1:30921860-30921879 MS.gene76584:CDS 35.0%
GGTACGATATGACTCAAAAA+AGG - chr5.1:30921436-30921455 None:intergenic 35.0%
GTATATTATCCTACTGCTGA+AGG + chr5.1:30920991-30921010 MS.gene76584:CDS 35.0%
TACACGATTCAGATATCAAG+GGG + chr5.1:30922095-30922114 MS.gene76584:CDS 35.0%
TCCCTCTATATCATTGTGTA+AGG - chr5.1:30921864-30921883 None:intergenic 35.0%
TCGAACAAAAAAGCTTAGGA+TGG + chr5.1:30921826-30921845 MS.gene76584:CDS 35.0%
TGCCTTACACAATGATATAG+AGG + chr5.1:30921859-30921878 MS.gene76584:CDS 35.0%
TGTAGTTAATGCTAAGGTTG+TGG + chr5.1:30921026-30921045 MS.gene76584:CDS 35.0%
! ATTAATTTTGCGCATTCGCT+AGG - chr5.1:30920652-30920671 None:intergenic 35.0%
!!! AAGTTTTTTGGTGTTGCAGT+TGG - chr5.1:30922020-30922039 None:intergenic 35.0%
!!! ATCCTAAGCTTTTTTGTTCG+AGG - chr5.1:30921827-30921846 None:intergenic 35.0%
!!! TCCTAAGCTTTTTTGTTCGA+GGG - chr5.1:30921826-30921845 None:intergenic 35.0%
!!! TGAACCAGGACTAAGTTTTT+TGG - chr5.1:30922032-30922051 None:intergenic 35.0%
ACACGATTCAGATATCAAGG+GGG + chr5.1:30922096-30922115 MS.gene76584:CDS 40.0%
ACTCCGTCTGATGCTAATAA+CGG + chr5.1:30920610-30920629 MS.gene76584:CDS 40.0%
AGTTAATGCTAAGGTTGTGG+AGG + chr5.1:30921029-30921048 MS.gene76584:CDS 40.0%
ATTATCCTACTGCTGAAGGT+TGG + chr5.1:30920995-30921014 MS.gene76584:CDS 40.0%
GGAATTGGCCGATAAGAAAA+TGG + chr5.1:30920957-30920976 MS.gene76584:CDS 40.0%
GTTGACGAGAAGAAACTGAT+TGG + chr5.1:30920814-30920833 MS.gene76584:CDS 40.0%
TCCCTCGAACAAAAAAGCTT+AGG + chr5.1:30921822-30921841 MS.gene76584:CDS 40.0%
TCGTGTAAATCACTTCCAAC+AGG - chr5.1:30922082-30922101 None:intergenic 40.0%
TGGATGTAGTGCATGTATGT+TGG - chr5.1:30921798-30921817 None:intergenic 40.0%
TTTCTTATCGGCCAATTCCA+CGG - chr5.1:30920956-30920975 None:intergenic 40.0%
!!! TTTTTTGTTCGAGGGAACTG+TGG - chr5.1:30921818-30921837 None:intergenic 40.0%
AAAATCTGTTACTGAGGCCG+TGG + chr5.1:30920936-30920955 MS.gene76584:CDS 45.0%
AACAAACTCACTCCAGTTGG+TGG - chr5.1:30920797-30920816 None:intergenic 45.0%
AGGAGTAGTTTGACGTTCGT+CGG - chr5.1:30920496-30920515 None:intergenic 45.0%
CCACCGTTATTAGCATCAGA+CGG - chr5.1:30920616-30920635 None:intergenic 45.0%
CTCTCAGTCACTGACATTCA+CGG + chr5.1:30920715-30920734 MS.gene76584:CDS 45.0%
CTGACATTCACGGCAAAGAA+TGG + chr5.1:30920725-30920744 MS.gene76584:CDS 45.0%
GAAATAAGCTCAACCTCCCA+AGG - chr5.1:30921776-30921795 None:intergenic 45.0%
GACGAGAAGAAACTGATTGG+TGG + chr5.1:30920817-30920836 MS.gene76584:CDS 45.0%
GGAACTGCAATAATCTCCGT+TGG + chr5.1:30920871-30920890 MS.gene76584:CDS 45.0%
GTCAACAAACTCACTCCAGT+TGG - chr5.1:30920800-30920819 None:intergenic 45.0%
GTTCGTGAAGACGAAGATGA+CGG + chr5.1:30920556-30920575 MS.gene76584:CDS 45.0%
TAATCTCCGTTGGAATCCGT+CGG + chr5.1:30920881-30920900 MS.gene76584:CDS 45.0%
TATCTGCTCCTAATCGTCCA+TGG + chr5.1:30921157-30921176 MS.gene76584:CDS 45.0%
TGAACCTAAAGTTTCGCAGC+TGG + chr5.1:30921733-30921752 MS.gene76584:CDS 45.0%
TGCGGAAAAAAGATTGTGCC+TGG - chr5.1:30921987-30922006 None:intergenic 45.0%
TTCGTGAAGACGAAGATGAC+GGG + chr5.1:30920557-30920576 MS.gene76584:CDS 45.0%
! CGGAGTTAAAGTTTTGCCAG+AGG - chr5.1:30920596-30920615 None:intergenic 45.0%
! TTTTGCGCATTCGCTAGGTA+CGG - chr5.1:30920647-30920666 None:intergenic 45.0%
!! GCAGTTGGAAGAAGTTGATG+CGG - chr5.1:30922005-30922024 None:intergenic 45.0%
CCGTCTGATGCTAATAACGG+TGG + chr5.1:30920613-30920632 MS.gene76584:CDS 50.0%
CGAACCAGCTGCGAAACTTT+AGG - chr5.1:30921740-30921759 None:intergenic 50.0%
CGATTCAGATATCAAGGGGG+AGG + chr5.1:30922099-30922118 MS.gene76584:CDS 50.0%
GCTTTGCTCGATGACATGCA+GGG + chr5.1:30921959-30921978 MS.gene76584:CDS 50.0%
GGTCAGATCATACGTCCTGT+TGG + chr5.1:30922064-30922083 MS.gene76584:CDS 50.0%
GTGACTGAGAGTTCCTGAGA+CGG - chr5.1:30920706-30920725 None:intergenic 50.0%
TATACCTCAAGCATGCGCCA+TGG - chr5.1:30921177-30921196 None:intergenic 50.0%
TCACACCAACCTTCAGCAGT+AGG - chr5.1:30921003-30921022 None:intergenic 50.0%
TCAGATATCAAGGGGGAGGA+TGG + chr5.1:30922103-30922122 MS.gene76584:CDS 50.0%
TCGTGAAGACGAAGATGACG+GGG + chr5.1:30920558-30920577 MS.gene76584:CDS 50.0%
TCTTCGTCTTCACGAACCTC+AGG - chr5.1:30920553-30920572 None:intergenic 50.0%
TGCTTTGCTCGATGACATGC+AGG + chr5.1:30921958-30921977 MS.gene76584:CDS 50.0%
TGTTACTGAGGCCGTGGAAT+TGG + chr5.1:30920942-30920961 MS.gene76584:CDS 50.0%
! GAGTTCCTGAGACGGCTTTT+CGG - chr5.1:30920698-30920717 None:intergenic 50.0%
AGCATGCGCCATGGACGATT+AGG - chr5.1:30921168-30921187 None:intergenic 55.0%
CAGCACCAAACTTCTGCCGA+CGG - chr5.1:30920900-30920919 None:intergenic 55.0%
CGGTGCAGGGAATGAATGGA+CGG - chr5.1:30920440-30920459 None:intergenic 55.0%
GACACCTGATCACCACCAAC+TGG + chr5.1:30920782-30920801 MS.gene76584:CDS 55.0%
GAGACGGTGCAGGGAATGAA+TGG - chr5.1:30920444-30920463 None:intergenic 55.0%
GAGTAGATCAACCGCGGAGA+CGG - chr5.1:30920460-30920479 None:intergenic 55.0%
GGAATCCGTCGGCAGAAGTT+TGG + chr5.1:30920892-30920911 MS.gene76584:CDS 55.0%
GGTGCAGGGAATGAATGGAC+GGG - chr5.1:30920439-30920458 None:intergenic 55.0%
GTCAGCGAGTAGATCAACCG+CGG - chr5.1:30920466-30920485 None:intergenic 55.0%
GTGGACACGGTCGTTAGTGA+CGG - chr5.1:30920524-30920543 None:intergenic 55.0%
TCGAGTGCGAGTCAGTCCTT+GGG + chr5.1:30921757-30921776 MS.gene76584:CDS 55.0%
TCGTCTTCACGAACCTCAGG+AGG - chr5.1:30920550-30920569 None:intergenic 55.0%
TGGACACGGTCGTTAGTGAC+GGG - chr5.1:30920523-30920542 None:intergenic 55.0%
TTCGAGTGCGAGTCAGTCCT+TGG + chr5.1:30921756-30921775 MS.gene76584:CDS 55.0%
! CTTCTGCCGACGGATTCCAA+CGG - chr5.1:30920890-30920909 None:intergenic 55.0%
! GGGGTTGACGTTGTTTCCTC+TGG + chr5.1:30920577-30920596 MS.gene76584:CDS 55.0%
! GGTCGTTAGTGACGGGAGTT+AGG - chr5.1:30920516-30920535 None:intergenic 55.0%
! GTGGTGATCAGGTGTCGCAA+GGG - chr5.1:30920778-30920797 None:intergenic 55.0%
! TTTTCGGCCTGGAGGTCAAG+TGG - chr5.1:30920682-30920701 None:intergenic 55.0%
!! CACTCCAGTTGGTGGTGATC+AGG - chr5.1:30920789-30920808 None:intergenic 55.0%
AAGCTTCGCCACGTCTACCG+TGG + chr5.1:30920748-30920767 MS.gene76584:CDS 60.0%
AGCTTCGCCACGTCTACCGT+GGG + chr5.1:30920749-30920768 MS.gene76584:CDS 60.0%
AGTGCGAGTCAGTCCTTGGG+AGG + chr5.1:30921760-30921779 MS.gene76584:CDS 60.0%
ATTCCCGCCACTTGACCTCC+AGG + chr5.1:30920672-30920691 MS.gene76584:CDS 60.0%
CACGAACCTCAGGAGGTTCG+TGG - chr5.1:30920543-30920562 None:intergenic 60.0%
CCTCAGGAGGTTCGTGGACA+CGG - chr5.1:30920537-30920556 None:intergenic 60.0%
TCGATGACATGCAGGGAGCC+AGG + chr5.1:30921966-30921985 MS.gene76584:CDS 60.0%
TCGTCCATGGCGCATGCTTG+AGG + chr5.1:30921170-30921189 MS.gene76584:CDS 60.0%
! GGTGGTGATCAGGTGTCGCA+AGG - chr5.1:30920779-30920798 None:intergenic 60.0%
AAGGGGGAGGATGGCCGTAG+AGG + chr5.1:30922112-30922131 MS.gene76584:CDS 65.0%
AGGGAGTCCCACGGTAGACG+TGG - chr5.1:30920759-30920778 None:intergenic 65.0%
ATCAACCGCGGAGACGGTGC+AGG - chr5.1:30920454-30920473 None:intergenic 65.0%
CATTCCCTGCACCGTCTCCG+CGG + chr5.1:30920446-30920465 MS.gene76584:CDS 65.0%
CCAGGCCGAAAAGCCGTCTC+AGG + chr5.1:30920690-30920709 MS.gene76584:CDS 65.0%
CCGTGTCCACGAACCTCCTG+AGG + chr5.1:30920534-30920553 MS.gene76584:CDS 65.0%
TCAACCGCGGAGACGGTGCA+GGG - chr5.1:30920453-30920472 None:intergenic 65.0%
TCGGCCTGGAGGTCAAGTGG+CGG - chr5.1:30920679-30920698 None:intergenic 65.0%
! CCTGAGACGGCTTTTCGGCC+TGG - chr5.1:30920693-30920712 None:intergenic 65.0%
! GAGACGGCTTTTCGGCCTGG+AGG - chr5.1:30920690-30920709 None:intergenic 65.0%
! GGTGTCGCAAGGGAGTCCCA+CGG - chr5.1:30920768-30920787 None:intergenic 65.0%
CGGCCTGGAGGTCAAGTGGC+GGG - chr5.1:30920678-30920697 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 30920430 30922159 30920430 ID=MS.gene76584
chr5.1 mRNA 30920430 30922159 30920430 ID=MS.gene76584.t1;Parent=MS.gene76584
chr5.1 exon 30920430 30921191 30920430 ID=MS.gene76584.t1.exon1;Parent=MS.gene76584.t1
chr5.1 CDS 30920430 30921191 30920430 ID=cds.MS.gene76584.t1;Parent=MS.gene76584.t1
chr5.1 exon 30921722 30922159 30921722 ID=MS.gene76584.t1.exon2;Parent=MS.gene76584.t1
chr5.1 CDS 30921722 30922159 30921722 ID=cds.MS.gene76584.t1;Parent=MS.gene76584.t1
Gene Sequence

>MS.gene76584

ATGGTTCCCGTCCATTCATTCCCTGCACCGTCTCCGCGGTTGATCTACTCGCTGACTCTCGTACCGACGAACGTCAAACTACTCCTAACTCCCGTCACTAACGACCGTGTCCACGAACCTCCTGAGGTTCGTGAAGACGAAGATGACGGGGTTGACGTTGTTTCCTCTGGCAAAACTTTAACTCCGTCTGATGCTAATAACGGTGGTGCATTCTCCGTACCTAGCGAATGCGCAAAATTAATATTCCCGCCACTTGACCTCCAGGCCGAAAAGCCGTCTCAGGAACTCTCAGTCACTGACATTCACGGCAAAGAATGGAAGCTTCGCCACGTCTACCGTGGGACTCCCTTGCGACACCTGATCACCACCAACTGGAGTGAGTTTGTTGACGAGAAGAAACTGATTGGTGGTGATTCCTTAGTTTTCATGAAAAAATCAACTGGAACTGCAATAATCTCCGTTGGAATCCGTCGGCAGAAGTTTGGTGCTGCTACTAAAATAACAGAAAAATCTGTTACTGAGGCCGTGGAATTGGCCGATAAGAAAATGGCTTTTGAAGTTGTATATTATCCTACTGCTGAAGGTTGGTGTGATTTTGTAGTTAATGCTAAGGTTGTGGAGGAAGCCATTAAGATTAAATGGAATCCTGGATTGAGAATTAAACATTCTTTGAAGAAAGACAATTCTTCAAAGAGATGCTCAAATTTTGAGGGAACAATATCTGCTCTATCTGCTCCTAATCGTCCATGGCGCATGCTTGAGGTATATATTTATATTTGCTCTCTTCTTTTAGTCTTGTTATATTTCAACTGCACAATGTTATATGTAGTATTTTTGTTTAATAAATGTTATCGGTCAAAGTTATGGTTCATGTTAGGAAACTATATTTTTTTGATCAGTTCATGTTAGGAACTATATGTTCCAATGTGAAAAATATAGGATATGTTCCAATGTGTTAATCTTAAACATTAATGTTGTGCAATTAAATTTAAGTGGCTTAATTCCTTTTTTGAGTCATATCGTACCACATCATGAATTAACTATAATGACTTTAGAAACTTTATACTTGAAATAGGTCGAATAAGTTTTTTTTTTTTTTTTTTAGCAAATGGTTGAATAAGTTGTCTCACTTGTGTGTTTACATATGTATTTGTACAATATACTTTGTTGGATTAAATAAGTTTCTCGTCCCTATAAATATAGCGAATTTTGATTTTAATCCTTAAAAAATAAAATGAGATGTTTTAAATGATTGATACGGATACTTATCATTTTTCATGTCAATATCATAGGTTAATTGGGATGAACCTAAAGTTTCGCAGCTGGTTCGAGTGCGAGTCAGTCCTTGGGAGGTTGAGCTTATTTCCAACATACATGCACTACATCCACAGTTCCCTCGAACAAAAAAGCTTAGGATGGCTAAAGATTCTGCCTTACACAATGATATAGAGGGAGACTCCTCCATTCCAAAGATAGAATTTCATTCTTCAGCGACGAGCTCCTTCAATGAAACATCGTTGAATAGCAATGCTTTGCTCGATGACATGCAGGGAGCCAGGCACAATCTTTTTTCCGCATCAACTTCTTCCAACTGCAACACCAAAAAACTTAGTCCTGGTTCAATTATGTTATTTGGTCAGATCATACGTCCTGTTGGAAGTGATTTACACGATTCAGATATCAAGGGGGAGGATGGCCGTAGAGGATGCAATGAAAATAGTCTTGTGTAG
Protein sequence

>MS.gene76584.t1

MVPVHSFPAPSPRLIYSLTLVPTNVKLLLTPVTNDRVHEPPEVREDEDDGVDVVSSGKTLTPSDANNGGAFSVPSECAKLIFPPLDLQAEKPSQELSVTDIHGKEWKLRHVYRGTPLRHLITTNWSEFVDEKKLIGGDSLVFMKKSTGTAIISVGIRRQKFGAATKITEKSVTEAVELADKKMAFEVVYYPTAEGWCDFVVNAKVVEEAIKIKWNPGLRIKHSLKKDNSSKRCSNFEGTISALSAPNRPWRMLEVNWDEPKVSQLVRVRVSPWEVELISNIHALHPQFPRTKKLRMAKDSALHNDIEGDSSIPKIEFHSSATSSFNETSLNSNALLDDMQGARHNLFSASTSSNCNTKKLSPGSIMLFGQIIRPVGSDLHDSDIKGEDGRRGCNENSLV