AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76602

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76602.t1 MTR_5g041350 87.819 353 42 1 1 352 1 353 0.0 610
MS.gene76602.t1 MTR_5g041350 85.171 263 38 1 91 352 2 264 6.97e-152 428
MS.gene76602.t1 MTR_5g041350 85.537 242 34 1 112 352 1 242 1.65e-139 395
MS.gene76602.t1 MTR_4g081710 59.143 350 127 5 2 349 4 339 4.61e-128 370
MS.gene76602.t1 MTR_2g086450 51.020 245 102 5 8 244 38 272 3.83e-70 224
MS.gene76602.t1 MTR_6g032990 49.565 230 109 3 8 233 35 261 6.56e-64 208
MS.gene76602.t1 MTR_6g444980 55.414 157 61 3 8 164 28 175 1.17e-49 171
MS.gene76602.t1 MTR_5g027440 44.865 185 94 4 5 186 15 194 3.64e-46 159
MS.gene76602.t1 MTR_6g444980 52.174 161 68 3 8 168 28 179 2.41e-45 158
MS.gene76602.t1 MTR_2g027800 41.949 236 116 7 8 230 32 259 2.78e-42 149
MS.gene76602.t1 MTR_7g088070 50.345 145 63 2 14 158 49 184 8.25e-42 147
MS.gene76602.t1 MTR_2g027800 41.202 233 108 7 8 230 32 245 6.96e-41 145
MS.gene76602.t1 MTR_7g088070 50.345 145 59 3 14 158 49 180 7.29e-41 145
MS.gene76602.t1 MTR_1g090670 49.296 142 69 1 8 149 37 175 4.76e-39 140
MS.gene76602.t1 MTR_2g027860 47.742 155 70 3 15 169 49 192 1.01e-37 138
MS.gene76602.t1 MTR_7g098250 43.537 147 67 3 11 155 258 390 1.08e-35 135
MS.gene76602.t1 MTR_1g080330 43.750 160 72 5 11 168 171 314 4.72e-35 132
MS.gene76602.t1 MTR_7g115530 37.234 188 94 3 15 200 218 383 5.96e-35 133
MS.gene76602.t1 MTR_1g053835 40.940 149 74 2 11 159 254 388 4.09e-32 125
MS.gene76602.t1 MTR_7g093030 35.849 159 85 2 14 170 211 354 6.37e-30 118
MS.gene76602.t1 MTR_1g080330 44.444 126 54 4 11 134 171 282 3.83e-27 108
MS.gene76602.t1 MTR_7g115530 42.742 124 55 2 14 135 217 326 3.01e-26 107
MS.gene76602.t1 MTR_7g068600 38.621 145 74 4 11 152 9 141 2.95e-24 99.8
MS.gene76602.t1 MTR_1g053800 38.255 149 73 2 8 153 170 302 7.67e-23 97.8
MS.gene76602.t1 MTR_7g115530 51.220 82 38 1 14 93 217 298 6.91e-22 94.7
MS.gene76602.t1 MTR_7g115530 58.621 58 24 0 14 71 217 274 1.01e-20 92.0
MS.gene76602.t1 MTR_7g115530 61.538 52 20 0 15 66 218 269 3.58e-19 86.7
MS.gene76602.t1 MTR_7g093030 55.172 58 26 0 14 71 211 268 3.88e-19 86.7
MS.gene76602.t1 MTR_1g053830 49.180 61 31 0 11 71 14 74 5.50e-19 81.3
MS.gene76602.t1 MTR_7g115530 58.491 53 22 0 15 67 218 270 2.66e-18 84.3
MS.gene76602.t1 MTR_1g112370 47.778 90 37 3 16 100 23 107 2.07e-17 82.4
MS.gene76602.t1 MTR_3g450310 45.556 90 46 2 15 101 19 108 2.97e-17 79.7
MS.gene76602.t1 MTR_0223s0040 50.000 76 35 1 16 88 19 94 8.19e-17 80.1
MS.gene76602.t1 MTR_7g069660 56.604 53 23 0 16 68 72 124 1.77e-16 80.1
MS.gene76602.t1 MTR_2g023580 51.667 60 29 0 16 75 56 115 2.67e-16 77.8
MS.gene76602.t1 MTR_1g090670 42.718 103 56 1 47 149 1 100 3.56e-16 77.0
MS.gene76602.t1 MTR_4g021790 58.182 55 22 1 15 69 168 221 5.84e-16 77.4
MS.gene76602.t1 MTR_2g016220 41.304 92 45 3 7 98 83 165 1.83e-15 77.0
MS.gene76602.t1 MTR_2g016220 41.304 92 45 3 7 98 83 165 2.00e-15 77.0
MS.gene76602.t1 MTR_6g071625 42.045 88 50 1 16 102 203 290 2.61e-15 76.6
MS.gene76602.t1 MTR_5g029470 44.872 78 43 0 16 93 220 297 2.90e-15 76.6
MS.gene76602.t1 MTR_2g016220 38.710 93 50 3 7 98 83 169 6.28e-15 75.5
MS.gene76602.t1 MTR_8g086410 52.830 53 25 0 16 68 112 164 6.86e-15 75.9
MS.gene76602.t1 MTR_8g086410 52.830 53 25 0 16 68 110 162 7.13e-15 75.9
MS.gene76602.t1 MTR_2g084230 52.830 53 25 0 16 68 98 150 1.05e-14 74.7
MS.gene76602.t1 MTR_2g084230 52.830 53 25 0 16 68 98 150 1.18e-14 73.9
MS.gene76602.t1 MTR_4g121020 57.627 59 24 1 11 69 211 268 1.31e-14 75.5
MS.gene76602.t1 MTR_4g121020 57.627 59 24 1 11 69 211 268 1.35e-14 75.5
MS.gene76602.t1 MTR_4g121020 57.627 59 24 1 11 69 107 164 1.49e-14 75.1
MS.gene76602.t1 MTR_4g021855 56.364 55 23 1 15 69 168 221 2.26e-14 73.2
MS.gene76602.t1 MTR_2g034960 54.286 70 25 2 11 74 207 275 2.28e-14 74.7
MS.gene76602.t1 MTR_5g017980 60.000 55 22 0 15 69 193 247 3.28e-14 73.2
MS.gene76602.t1 MTR_8g066310 45.070 71 39 0 16 86 174 244 3.87e-14 73.2
MS.gene76602.t1 MTR_8g086410 44.615 65 32 1 16 76 112 176 4.68e-14 73.2
MS.gene76602.t1 MTR_3g064840 52.632 57 27 0 16 72 275 331 8.93e-14 72.4
MS.gene76602.t1 MTR_1g032570 54.237 59 26 1 11 69 127 184 1.01e-13 72.4
MS.gene76602.t1 MTR_1g032570 54.237 59 26 1 11 69 196 253 1.06e-13 72.4
MS.gene76602.t1 MTR_1g021520 39.773 88 45 1 16 95 127 214 1.07e-13 71.2
MS.gene76602.t1 MTR_4g086835 55.172 58 26 0 12 69 271 328 1.21e-13 72.0
MS.gene76602.t1 MTR_4g021760 55.556 54 23 1 15 68 169 221 1.30e-13 71.2
MS.gene76602.t1 MTR_7g089010 56.364 55 23 1 15 69 91 144 1.82e-13 70.5
MS.gene76602.t1 MTR_2g450070 52.542 59 27 1 11 69 209 266 2.15e-13 71.6
MS.gene76602.t1 MTR_2g450070 52.542 59 27 1 11 69 209 266 2.17e-13 71.6
MS.gene76602.t1 MTR_4g064730 53.448 58 26 1 12 69 128 184 2.80e-13 70.1
MS.gene76602.t1 MTR_4g113140 53.448 58 27 0 12 69 232 289 6.12e-13 69.7
MS.gene76602.t1 MTR_1g093080 60.000 55 22 0 15 69 211 265 6.90e-13 69.3
MS.gene76602.t1 MTR_4g021845 56.863 51 21 1 15 65 168 217 7.09e-13 67.8
MS.gene76602.t1 MTR_5g017980 60.000 55 21 1 15 69 193 246 7.56e-13 68.9
MS.gene76602.t1 MTR_5g017980 60.000 55 21 1 15 69 192 245 8.17e-13 68.9
MS.gene76602.t1 MTR_4g086835 55.172 58 25 1 12 69 271 327 1.88e-12 68.2
MS.gene76602.t1 MTR_7g093010 33.577 137 78 3 15 150 213 337 2.33e-12 67.8
MS.gene76602.t1 MTR_7g026400 52.727 55 25 1 15 69 157 210 1.85e-11 65.5
MS.gene76602.t1 MTR_4g131600 49.153 59 29 1 11 69 197 254 2.19e-11 65.5
MS.gene76602.t1 MTR_5g054300 54.545 55 25 0 15 69 198 252 2.40e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76602.t1 AT1G69580 50.000 266 116 5 6 262 25 282 3.58e-76 238
MS.gene76602.t1 AT1G69580 49.438 267 117 5 6 262 25 283 5.49e-75 235
MS.gene76602.t1 AT3G04030 54.077 233 97 4 8 235 38 265 7.01e-69 221
MS.gene76602.t1 AT5G18240 52.361 233 101 4 8 235 38 265 6.95e-68 219
MS.gene76602.t1 AT5G18240 52.361 233 101 4 8 235 38 265 6.95e-68 219
MS.gene76602.t1 AT3G04030 53.219 233 98 5 8 235 38 264 1.01e-65 213
MS.gene76602.t1 AT5G18240 51.502 233 101 5 8 235 38 263 1.83e-64 210
MS.gene76602.t1 AT3G04030 51.931 233 96 5 8 235 38 259 6.73e-62 203
MS.gene76602.t1 AT5G18240 50.215 233 100 5 8 235 38 259 4.10e-61 201
MS.gene76602.t1 AT5G18240 50.215 233 100 5 8 235 38 259 4.10e-61 201
MS.gene76602.t1 AT3G24120 45.133 226 116 4 8 226 34 258 1.89e-56 186
MS.gene76602.t1 AT4G13640 44.872 234 107 6 8 229 30 253 8.85e-56 184
MS.gene76602.t1 AT3G24120 44.541 229 116 5 8 226 34 261 3.42e-54 180
MS.gene76602.t1 AT4G13640 44.304 237 107 7 8 229 30 256 1.25e-53 179
MS.gene76602.t1 AT1G79430 56.051 157 60 3 8 164 27 174 1.62e-50 172
MS.gene76602.t1 AT5G45580 44.512 164 85 2 9 166 1 164 1.01e-42 149
MS.gene76602.t1 AT5G45580 44.512 164 85 2 9 166 17 180 1.74e-42 149
MS.gene76602.t1 AT3G04030 48.969 194 89 4 47 235 1 189 2.93e-41 147
MS.gene76602.t1 AT2G01060 48.052 154 68 4 14 167 14 155 1.09e-39 142
MS.gene76602.t1 AT3G12730 48.052 154 72 3 8 159 16 163 1.11e-39 140
MS.gene76602.t1 AT3G04450 42.568 148 76 2 11 158 194 332 6.60e-35 132
MS.gene76602.t1 AT3G04450 42.568 148 76 2 11 158 194 332 6.60e-35 132
MS.gene76602.t1 AT5G29000 43.333 150 66 3 14 158 187 322 8.76e-35 131
MS.gene76602.t1 AT5G29000 43.333 150 66 3 14 158 187 322 8.76e-35 131
MS.gene76602.t1 AT3G04450 42.568 148 76 2 11 158 234 372 1.11e-34 132
MS.gene76602.t1 AT3G04030 46.392 194 88 5 47 235 1 183 1.39e-34 129
MS.gene76602.t1 AT5G29000 43.333 150 66 3 14 158 230 365 1.46e-34 131
MS.gene76602.t1 AT5G18240 45.361 194 90 5 47 235 1 183 1.58e-34 129
MS.gene76602.t1 AT4G28610 41.216 148 76 2 14 161 224 360 5.64e-34 129
MS.gene76602.t1 AT5G06800 41.667 144 76 2 15 158 192 327 6.57e-32 123
MS.gene76602.t1 AT5G06800 41.667 144 71 3 15 158 192 322 4.14e-31 121
MS.gene76602.t1 AT3G13040 43.919 148 66 4 11 153 237 372 9.69e-30 119
MS.gene76602.t1 AT3G13040 43.919 148 66 4 11 153 237 372 9.69e-30 119
MS.gene76602.t1 AT3G13040 43.919 148 66 4 11 153 237 372 9.69e-30 119
MS.gene76602.t1 AT2G20400 41.176 153 69 3 15 162 231 367 5.07e-29 116
MS.gene76602.t1 AT2G20400 40.523 153 69 3 15 162 231 366 1.42e-28 114
MS.gene76602.t1 AT1G79430 51.695 118 48 3 47 164 1 109 2.67e-27 109
MS.gene76602.t1 AT3G04450 57.377 61 26 0 11 71 234 294 7.21e-21 92.0
MS.gene76602.t1 AT5G29000 56.897 58 25 0 14 71 230 287 1.94e-20 90.9
MS.gene76602.t1 AT2G01060 44.068 118 54 4 50 167 1 106 5.24e-20 88.2
MS.gene76602.t1 AT2G06020 54.098 61 28 0 11 71 82 142 2.37e-18 84.7
MS.gene76602.t1 AT5G06800 56.140 57 25 0 15 71 192 248 4.25e-18 83.6
MS.gene76602.t1 AT1G14600 58.491 53 22 0 16 68 24 76 1.42e-17 81.6
MS.gene76602.t1 AT2G02060 52.459 61 29 0 16 76 30 90 8.57e-17 79.3
MS.gene76602.t1 AT2G02060 52.459 61 29 0 16 76 30 90 8.57e-17 79.3
MS.gene76602.t1 AT3G12730 41.739 115 59 3 47 159 1 109 9.41e-17 77.8
MS.gene76602.t1 AT1G32240 44.872 78 43 0 16 93 214 291 7.71e-16 78.6
MS.gene76602.t1 AT1G32240 44.872 78 43 0 16 93 214 291 8.69e-16 78.2
MS.gene76602.t1 AT2G38300 56.604 53 23 0 16 68 55 107 1.44e-15 77.0
MS.gene76602.t1 AT4G04605 52.830 53 25 0 16 68 15 67 1.84e-15 75.1
MS.gene76602.t1 AT2G42660 50.000 64 29 1 16 79 51 111 5.81e-15 74.3
MS.gene76602.t1 AT2G40260 50.847 59 29 0 16 74 83 141 9.80e-15 75.1
MS.gene76602.t1 AT4G17695 45.570 79 37 1 16 88 165 243 1.67e-14 73.9
MS.gene76602.t1 AT5G16560 52.632 57 27 0 16 72 220 276 2.85e-14 73.9
MS.gene76602.t1 AT5G59570 53.448 58 26 1 12 69 138 194 3.23e-14 72.8
MS.gene76602.t1 AT5G59570 53.448 58 26 1 12 69 138 194 3.23e-14 72.8
MS.gene76602.t1 AT2G40970 55.172 58 25 1 12 69 102 158 6.02e-14 71.2
MS.gene76602.t1 AT5G05090 56.364 55 23 1 15 69 81 134 6.16e-14 71.6
MS.gene76602.t1 AT5G42630 51.786 56 27 0 16 71 106 161 7.65e-14 70.5
MS.gene76602.t1 AT3G10760 56.364 55 23 1 15 69 105 158 7.76e-14 72.0
MS.gene76602.t1 AT3G16857 56.364 55 23 1 15 69 167 220 9.96e-14 72.8
MS.gene76602.t1 AT3G16857 56.364 55 23 1 15 69 237 290 1.14e-13 72.8
MS.gene76602.t1 AT3G16857 56.364 55 23 1 15 69 237 290 1.19e-13 72.4
MS.gene76602.t1 AT5G42630 51.786 56 27 0 16 71 106 161 1.21e-13 70.9
MS.gene76602.t1 AT3G46640 54.545 55 24 1 15 69 144 197 1.22e-13 71.2
MS.gene76602.t1 AT3G46640 54.545 55 24 1 15 69 144 197 1.22e-13 71.2
MS.gene76602.t1 AT3G46640 54.545 55 24 1 15 69 144 197 1.40e-13 71.2
MS.gene76602.t1 AT1G25550 55.172 58 26 0 12 69 206 263 3.33e-13 70.1
MS.gene76602.t1 AT1G49560 60.000 55 22 0 15 69 192 246 3.59e-13 70.1
MS.gene76602.t1 AT1G68670 56.897 58 25 0 12 69 212 269 6.25e-13 69.3
MS.gene76602.t1 AT3G25790 49.206 63 32 0 20 82 201 263 6.53e-13 69.3
MS.gene76602.t1 AT4G04580 45.588 68 36 1 1 68 1 67 1.34e-12 65.5
MS.gene76602.t1 AT2G03500 51.724 58 28 0 12 69 230 287 2.68e-12 67.8
MS.gene76602.t1 AT4G37180 52.830 53 25 0 17 69 212 264 3.05e-12 67.4
MS.gene76602.t1 AT4G37180 52.830 53 25 0 17 69 219 271 3.46e-12 67.4
MS.gene76602.t1 AT5G45580 34.545 110 66 2 63 166 16 125 6.67e-12 65.1
MS.gene76602.t1 AT5G58080 54.237 59 26 1 11 69 190 247 8.73e-12 66.6
MS.gene76602.t1 AT2G01760 50.909 55 26 1 15 69 135 188 9.08e-12 65.9
MS.gene76602.t1 AT2G01760 50.909 55 26 1 15 69 200 253 1.41e-11 65.5
MS.gene76602.t1 AT1G67710 45.588 68 32 1 17 79 121 188 1.71e-11 65.5
MS.gene76602.t1 AT2G01760 50.909 55 26 1 15 69 228 281 1.95e-11 65.1
MS.gene76602.t1 AT1G13300 46.269 67 36 0 15 81 181 247 2.26e-11 64.7
MS.gene76602.t1 AT1G67710 45.588 68 32 1 17 79 195 262 2.28e-11 65.5
MS.gene76602.t1 AT1G67710 45.588 68 32 1 17 79 130 197 2.55e-11 65.1
MS.gene76602.t1 AT3G62670 46.774 62 29 1 11 68 207 268 4.88e-11 63.9
MS.gene76602.t1 AT5G44190 50.000 54 26 1 15 68 147 199 5.23e-11 63.9
MS.gene76602.t1 AT5G44190 50.000 54 26 1 15 68 147 199 6.66e-11 63.2
MS.gene76602.t1 AT1G49560 50.000 64 30 1 15 78 192 253 8.90e-11 62.0
MS.gene76602.t1 AT1G25550 51.852 54 26 0 12 65 206 259 9.95e-11 62.4

Find 60 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACTCAGTGATTTGATTC+TGG 0.268350 5.1:+31170907 None:intergenic
TGGAACTTCTTTGTGTTCAT+TGG 0.299674 5.1:-31170216 MS.gene76602:CDS
TTCACTTGAATTGCCACTAA+TGG 0.314380 5.1:-31170105 MS.gene76602:CDS
CAGAGTAGGTTTCTAGTTGT+TGG 0.316123 5.1:+31171245 None:intergenic
AAAATGGGAATACCTGTAAA+TGG 0.318364 5.1:+31171367 None:intergenic
TAATTGCATCAATGAATCTT+TGG 0.326033 5.1:+31171594 None:intergenic
AAACATTCAATAGAAGAAAT+AGG 0.327972 5.1:-31170152 MS.gene76602:CDS
ATCACCCTCATAATACTCTT+TGG 0.337237 5.1:+31171426 None:intergenic
AGTCCTTGTCATTCTGATAT+TGG 0.344487 5.1:+31169877 None:intergenic
ATGAATCTTTGGTGAAGTTC+AGG 0.353886 5.1:+31171605 None:intergenic
CCATCTCAAGAGCCATTTAC+AGG 0.357774 5.1:-31171379 MS.gene76602:intron
AATGAAGAAACTAAATTTGA+TGG 0.358160 5.1:-31169987 MS.gene76602:CDS
ATGCAAAACCAAGGCTCAAA+TGG 0.392729 5.1:-31171631 MS.gene76602:CDS
GCATTGTTCATCACAGCTCT+TGG 0.396519 5.1:+31170953 None:intergenic
AAGGCGCAGGAGGCCCTCTC+TGG 0.408389 5.1:-31170392 MS.gene76602:CDS
GGATACAATTCTTCACCAAT+TGG 0.409885 5.1:-31170371 MS.gene76602:CDS
TTGTTCTTTGTTGTCAGAGT+AGG 0.427163 5.1:+31171231 None:intergenic
AAAAGACGAACTGTCTCAAT+TGG 0.442895 5.1:-31170336 MS.gene76602:CDS
GCAGCAGAGAAATCAGTCTT+GGG 0.443255 5.1:-31170932 MS.gene76602:CDS
GATTCTGTTAGATCTGAGAT+TGG 0.449789 5.1:+31170281 None:intergenic
TCTGAGTGCATGGCCATTAG+TGG 0.458598 5.1:+31170092 None:intergenic
AAACTATGAGGAGAGGAAAC+AGG 0.459208 5.1:-31170243 MS.gene76602:CDS
TCAGGAGTCCATTTGAGCCT+TGG 0.464509 5.1:+31171623 None:intergenic
TGCAGCAGAGAAATCAGTCT+TGG 0.471008 5.1:-31170933 MS.gene76602:CDS
ATGAGGGTGATGGAAATTCC+AGG 0.471652 5.1:-31171414 MS.gene76602:CDS
ATTGATGCAATTAATCAACT+TGG 0.481050 5.1:-31171584 MS.gene76602:CDS
ATCTTCTGAAATCTCAGAAA+GGG 0.484544 5.1:-31170180 MS.gene76602:CDS
AGATCTGAGATTGGAGAACT+TGG 0.485427 5.1:+31170290 None:intergenic
CAACACCAAAGAGTATTATG+AGG 0.491136 5.1:-31171431 MS.gene76602:CDS
CATCTTCTGAAATCTCAGAA+AGG 0.495942 5.1:-31170181 MS.gene76602:CDS
TTTACAATCGGTACTAATGA+AGG 0.497419 5.1:-31170411 MS.gene76602:CDS
ATTTAAGTAATGAAGTCAAT+GGG 0.515023 5.1:-31170019 MS.gene76602:CDS
AATTTAAGTAATGAAGTCAA+TGG 0.515931 5.1:-31170020 MS.gene76602:CDS
TCAAGGAAAATATTTACAAT+CGG 0.522599 5.1:-31170423 MS.gene76602:CDS
AGATGGTACAAAGTAAGTCC+TGG 0.523577 5.1:+31171396 None:intergenic
GAAACTTAATGAACAAATTG+AGG 0.524227 5.1:-31170730 MS.gene76602:intron
GATGCAATTAATCAACTTGG+AGG 0.524466 5.1:-31171581 MS.gene76602:CDS
AAATGCAAGTGGAAGTAGAA+AGG 0.528516 5.1:-31170752 MS.gene76602:CDS
TGATTTCTCTTCTGAGTGCA+TGG 0.534501 5.1:+31170082 None:intergenic
CGAAGCACAGACACAGACAT+TGG 0.544995 5.1:+31170050 None:intergenic
CCTGTAAATGGCTCTTGAGA+TGG 0.548306 5.1:+31171379 None:intergenic
AAAGAGTATTATGAGGGTGA+TGG 0.553041 5.1:-31171424 MS.gene76602:CDS
AGGGTTAAGTTTAAACTATG+AGG 0.556660 5.1:-31170255 MS.gene76602:CDS
CAGCTTCGAATCGAAGCTCA+AGG 0.569311 5.1:-31170440 MS.gene76602:CDS
TAAGTTTAAACTATGAGGAG+AGG 0.577698 5.1:-31170250 MS.gene76602:CDS
TCATGCTCTAGAAATGCAAG+TGG 0.579389 5.1:-31170763 MS.gene76602:CDS
ATCGGTACTAATGAAGGCGC+AGG 0.585210 5.1:-31170405 MS.gene76602:CDS
GGTTGTAGAAATATAGACTT+GGG 0.601442 5.1:-31171273 MS.gene76602:intron
GTGAAGAATTGTATCCAGAG+AGG 0.609917 5.1:+31170378 None:intergenic
TGGTTGTAGAAATATAGACT+TGG 0.620704 5.1:-31171274 MS.gene76602:intron
TCAGATCTAACAGAATCAAG+AGG 0.622910 5.1:-31170275 MS.gene76602:CDS
AACACCAAAGAGTATTATGA+GGG 0.627296 5.1:-31171430 MS.gene76602:CDS
TGAAGAATTGTATCCAGAGA+GGG 0.628116 5.1:+31170379 None:intergenic
TACTCTGACAACAAAGAACA+AGG 0.633344 5.1:-31171229 MS.gene76602:intron
CAGATCTAACAGAATCAAGA+GGG 0.636389 5.1:-31170274 MS.gene76602:CDS
GAGGAGAGGAAACAGGACAA+TGG 0.639420 5.1:-31170236 MS.gene76602:CDS
GGTACTAATGAAGGCGCAGG+AGG 0.653630 5.1:-31170402 MS.gene76602:CDS
CAGCAGAGAAATCAGTCTTG+GGG 0.657853 5.1:-31170931 MS.gene76602:CDS
TAGCCAATATCAGAATGACA+AGG 0.715504 5.1:-31169880 MS.gene76602:CDS
TATCCACTGATGCAAAACCA+AGG 0.720964 5.1:-31171640 MS.gene76602:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATGAAAAAAAATAGTA+AGG + chr5.1:31169966-31169985 None:intergenic 10.0%
!! AATTATTCATTAAGAAAAAG+AGG - chr5.1:31171535-31171554 MS.gene76602:intron 15.0%
!! TACAATATCATTACAAAAAT+GGG + chr5.1:31170137-31170156 None:intergenic 15.0%
!! TTACAATATCATTACAAAAA+TGG + chr5.1:31170138-31170157 None:intergenic 15.0%
!!! AAAATAATTTTAGATTGAAC+TGG - chr5.1:31170644-31170663 MS.gene76602:intron 15.0%
!!! GTAATGATATTGTAATTTTT+TGG - chr5.1:31170143-31170162 MS.gene76602:CDS 15.0%
!!! TTTTTTTTTTACCAAAATCA+CGG + chr5.1:31170889-31170908 None:intergenic 15.0%
!! AAACATTCAATAGAAGAAAT+AGG - chr5.1:31171334-31171353 MS.gene76602:intron 20.0%
!! AATCCAAATGATTGATTATT+TGG - chr5.1:31170470-31170489 MS.gene76602:intron 20.0%
!! AATGAAGAAACTAAATTTGA+TGG - chr5.1:31171499-31171518 MS.gene76602:intron 20.0%
!! AATTTAAGTAATGAAGTCAA+TGG - chr5.1:31171466-31171485 MS.gene76602:intron 20.0%
!! ATTTAAGTAATGAAGTCAAT+GGG - chr5.1:31171467-31171486 MS.gene76602:intron 20.0%
!! TCAAGGAAAATATTTACAAT+CGG - chr5.1:31171063-31171082 MS.gene76602:intron 20.0%
! ATGCCAAATAATCAATCATT+TGG + chr5.1:31170476-31170495 None:intergenic 25.0%
! ATGTATATGTGATGAAATCA+CGG + chr5.1:31170945-31170964 None:intergenic 25.0%
! ATTATTTGGCATAACATTGA+TGG - chr5.1:31170484-31170503 MS.gene76602:intron 25.0%
! ATTGATGCAATTAATCAACT+TGG - chr5.1:31169902-31169921 MS.gene76602:CDS 25.0%
! CCGATAATTTAAAAATCAGA+CGG + chr5.1:31170413-31170432 None:intergenic 25.0%
! GAAACTTAATGAACAAATTG+AGG - chr5.1:31170756-31170775 MS.gene76602:CDS 25.0%
! TAATTGCATCAATGAATCTT+TGG + chr5.1:31169895-31169914 None:intergenic 25.0%
! TATTCATTAAGAAAAAGAGG+TGG - chr5.1:31171538-31171557 MS.gene76602:intron 25.0%
! TCAAATTGTGTATGTTAATG+TGG - chr5.1:31170026-31170045 MS.gene76602:CDS 25.0%
!! CCGTCTGATTTTTAAATTAT+CGG - chr5.1:31170410-31170429 MS.gene76602:CDS 25.0%
!!! GACTTTTCATGTTTTTTTAC+TGG - chr5.1:31170192-31170211 MS.gene76602:CDS 25.0%
!!! TTTGTCAGTTTTATTCCAAT+TGG + chr5.1:31171133-31171152 None:intergenic 25.0%
AAAATGGGAATACCTGTAAA+TGG + chr5.1:31170122-31170141 None:intergenic 30.0%
AGGGTTAAGTTTAAACTATG+AGG - chr5.1:31171231-31171250 MS.gene76602:CDS 30.0%
ATTACTCAGTGATTTGATTC+TGG + chr5.1:31170582-31170601 None:intergenic 30.0%
GGTTGTAGAAATATAGACTT+GGG - chr5.1:31170213-31170232 MS.gene76602:CDS 30.0%
TAAGGACGTGTTAATCAATT+TGG - chr5.1:31170995-31171014 MS.gene76602:intron 30.0%
TAAGTTTAAACTATGAGGAG+AGG - chr5.1:31171236-31171255 MS.gene76602:CDS 30.0%
TATGCACTAAATGTTGCATA+AGG - chr5.1:31170977-31170996 MS.gene76602:intron 30.0%
TCAATTTGGTTGTTCATTTC+AGG - chr5.1:31171009-31171028 MS.gene76602:intron 30.0%
TGCAACATTTAGTGCATATT+TGG + chr5.1:31170975-31170994 None:intergenic 30.0%
TGGTTGTAGAAATATAGACT+TGG - chr5.1:31170212-31170231 MS.gene76602:CDS 30.0%
TTTACAATCGGTACTAATGA+AGG - chr5.1:31171075-31171094 MS.gene76602:intron 30.0%
! CTAACTTCCAAGTACATTTT+TGG - chr5.1:31170828-31170847 MS.gene76602:intron 30.0%
!! AACACCAAAGAGTATTATGA+GGG - chr5.1:31170056-31170075 MS.gene76602:CDS 30.0%
!! ATCTTCTGAAATCTCAGAAA+GGG - chr5.1:31171306-31171325 MS.gene76602:intron 30.0%
!! TTCCAAGTACATTTTTGGAT+TGG - chr5.1:31170833-31170852 MS.gene76602:intron 30.0%
AAAAGACGAACTGTCTCAAT+TGG - chr5.1:31171150-31171169 MS.gene76602:intron 35.0%
AAATGCAAGTGGAAGTAGAA+AGG - chr5.1:31170734-31170753 MS.gene76602:CDS 35.0%
ACCATCTCGTATCAAAATAG+TGG - chr5.1:31170340-31170359 MS.gene76602:CDS 35.0%
AGTCCTTGTCATTCTGATAT+TGG + chr5.1:31171612-31171631 None:intergenic 35.0%
ATCACCCTCATAATACTCTT+TGG + chr5.1:31170063-31170082 None:intergenic 35.0%
ATGAATCTTTGGTGAAGTTC+AGG + chr5.1:31169884-31169903 None:intergenic 35.0%
ATGAGATATCTCCATGTTTC+TGG + chr5.1:31170707-31170726 None:intergenic 35.0%
CAGATCTAACAGAATCAAGA+GGG - chr5.1:31171212-31171231 MS.gene76602:intron 35.0%
GATGCAATTAATCAACTTGG+AGG - chr5.1:31169905-31169924 MS.gene76602:CDS 35.0%
GGATACAATTCTTCACCAAT+TGG - chr5.1:31171115-31171134 MS.gene76602:intron 35.0%
TACTCTGACAACAAAGAACA+AGG - chr5.1:31170257-31170276 MS.gene76602:CDS 35.0%
TAGCCAATATCAGAATGACA+AGG - chr5.1:31171606-31171625 MS.gene76602:CDS 35.0%
TATATGTGATGAAATCACGG+TGG + chr5.1:31170942-31170961 None:intergenic 35.0%
TCAGATCTAACAGAATCAAG+AGG - chr5.1:31171211-31171230 MS.gene76602:intron 35.0%
TGAAGAATTGTATCCAGAGA+GGG + chr5.1:31171110-31171129 None:intergenic 35.0%
TGGAACTTCTTTGTGTTCAT+TGG - chr5.1:31171270-31171289 MS.gene76602:intron 35.0%
TTCACTTGAATTGCCACTAA+TGG - chr5.1:31171381-31171400 MS.gene76602:CDS 35.0%
! GATTCTGTTAGATCTGAGAT+TGG + chr5.1:31171208-31171227 None:intergenic 35.0%
! TTGTTCTTTGTTGTCAGAGT+AGG + chr5.1:31170258-31170277 None:intergenic 35.0%
!! AAAGAGTATTATGAGGGTGA+TGG - chr5.1:31170062-31170081 MS.gene76602:CDS 35.0%
!! CAACACCAAAGAGTATTATG+AGG - chr5.1:31170055-31170074 MS.gene76602:CDS 35.0%
!! CAAGTACATTTTTGGATTGG+CGG - chr5.1:31170836-31170855 MS.gene76602:intron 35.0%
!! CATAACATTGATGGTCCTTT+TGG - chr5.1:31170493-31170512 MS.gene76602:intron 35.0%
!! CATCTTCTGAAATCTCAGAA+AGG - chr5.1:31171305-31171324 MS.gene76602:intron 35.0%
!!! ACCACTATTTTGATACGAGA+TGG + chr5.1:31170344-31170363 None:intergenic 35.0%
!!! TTCTGTTTTTGCCAGAAACA+TGG - chr5.1:31170693-31170712 MS.gene76602:intron 35.0%
AAACTATGAGGAGAGGAAAC+AGG - chr5.1:31171243-31171262 MS.gene76602:CDS 40.0%
AGATGGTACAAAGTAAGTCC+TGG + chr5.1:31170093-31170112 None:intergenic 40.0%
ATGCAAAACCAAGGCTCAAA+TGG - chr5.1:31169855-31169874 MS.gene76602:CDS 40.0%
CGCCAATCCAAAAATGTACT+TGG + chr5.1:31170838-31170857 None:intergenic 40.0%
GTGAAGAATTGTATCCAGAG+AGG + chr5.1:31171111-31171130 None:intergenic 40.0%
TATCCACTGATGCAAAACCA+AGG - chr5.1:31169846-31169865 MS.gene76602:CDS 40.0%
TGATTTCTCTTCTGAGTGCA+TGG + chr5.1:31171407-31171426 None:intergenic 40.0%
! AGAACAAGGTGATGACTTGT+AGG - chr5.1:31170271-31170290 MS.gene76602:CDS 40.0%
! AGATCTGAGATTGGAGAACT+TGG + chr5.1:31171199-31171218 None:intergenic 40.0%
! GAACAAGGTGATGACTTGTA+GGG - chr5.1:31170272-31170291 MS.gene76602:CDS 40.0%
!! CAGAGTAGGTTTCTAGTTGT+TGG + chr5.1:31170244-31170263 None:intergenic 40.0%
!! TCATGCTCTAGAAATGCAAG+TGG - chr5.1:31170723-31170742 MS.gene76602:intron 40.0%
CAGCAGAGAAATCAGTCTTG+GGG - chr5.1:31170555-31170574 MS.gene76602:intron 45.0%
CCATCTCAAGAGCCATTTAC+AGG - chr5.1:31170107-31170126 MS.gene76602:CDS 45.0%
CCTGTAAATGGCTCTTGAGA+TGG + chr5.1:31170110-31170129 None:intergenic 45.0%
CGGTGAGTTCAGTAGAATCA+TGG - chr5.1:31170856-31170875 MS.gene76602:intron 45.0%
GCAGCAGAGAAATCAGTCTT+GGG - chr5.1:31170554-31170573 MS.gene76602:intron 45.0%
GCATTGTTCATCACAGCTCT+TGG + chr5.1:31170536-31170555 None:intergenic 45.0%
TCTGTGTACACTGCACCAAA+AGG + chr5.1:31170511-31170530 None:intergenic 45.0%
TGCAGCAGAGAAATCAGTCT+TGG - chr5.1:31170553-31170572 MS.gene76602:intron 45.0%
! ATGGTGTGCTACCGTGATTT+TGG - chr5.1:31170875-31170894 MS.gene76602:intron 45.0%
!! ATGAGGGTGATGGAAATTCC+AGG - chr5.1:31170072-31170091 MS.gene76602:CDS 45.0%
CAGCTTCGAATCGAAGCTCA+AGG - chr5.1:31171046-31171065 MS.gene76602:intron 50.0%
CGAAGCACAGACACAGACAT+TGG + chr5.1:31171439-31171458 None:intergenic 50.0%
GAGGAGAGGAAACAGGACAA+TGG - chr5.1:31171250-31171269 MS.gene76602:CDS 50.0%
TCAGGAGTCCATTTGAGCCT+TGG + chr5.1:31169866-31169885 None:intergenic 50.0%
TCTGAGTGCATGGCCATTAG+TGG + chr5.1:31171397-31171416 None:intergenic 50.0%
! ATCGGTACTAATGAAGGCGC+AGG - chr5.1:31171081-31171100 MS.gene76602:intron 50.0%
!!! GAGCCTTGGTTTTGCATCAG+TGG + chr5.1:31169852-31169871 None:intergenic 50.0%
! GGTACTAATGAAGGCGCAGG+AGG - chr5.1:31171084-31171103 MS.gene76602:intron 55.0%
AAGGCGCAGGAGGCCCTCTC+TGG - chr5.1:31171094-31171113 MS.gene76602:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 31169818 31171690 31169818 ID=MS.gene76602
chr5.1 mRNA 31169818 31171690 31169818 ID=MS.gene76602.t1;Parent=MS.gene76602
chr5.1 exon 31171573 31171690 31171573 ID=MS.gene76602.t1.exon1;Parent=MS.gene76602.t1
chr5.1 CDS 31171573 31171690 31171573 ID=cds.MS.gene76602.t1;Parent=MS.gene76602.t1
chr5.1 exon 31171380 31171456 31171380 ID=MS.gene76602.t1.exon2;Parent=MS.gene76602.t1
chr5.1 CDS 31171380 31171456 31171380 ID=cds.MS.gene76602.t1;Parent=MS.gene76602.t1
chr5.1 exon 31171230 31171287 31171230 ID=MS.gene76602.t1.exon3;Parent=MS.gene76602.t1
chr5.1 CDS 31171230 31171287 31171230 ID=cds.MS.gene76602.t1;Parent=MS.gene76602.t1
chr5.1 exon 31170912 31170987 31170912 ID=MS.gene76602.t1.exon4;Parent=MS.gene76602.t1
chr5.1 CDS 31170912 31170987 31170912 ID=cds.MS.gene76602.t1;Parent=MS.gene76602.t1
chr5.1 exon 31170731 31170800 31170731 ID=MS.gene76602.t1.exon5;Parent=MS.gene76602.t1
chr5.1 CDS 31170731 31170800 31170731 ID=cds.MS.gene76602.t1;Parent=MS.gene76602.t1
chr5.1 exon 31169818 31170477 31169818 ID=MS.gene76602.t1.exon6;Parent=MS.gene76602.t1
chr5.1 CDS 31169818 31170477 31169818 ID=cds.MS.gene76602.t1;Parent=MS.gene76602.t1
Gene Sequence

>MS.gene76602

ATGCAGAATCAAAGTATGCATTTTGTTCTATCCACTGATGCAAAACCAAGGCTCAAATGGACTCCTGAACTTCACCAAAGATTCATTGATGCAATTAATCAACTTGGAGGTGCAGCTAGTAAGTACAAAACAAACTCAATAATGACCTTACTATTTTTTTTCATATTTTTATTACACTTTAGAGCATAAATGTTACTTAATGTATCATTCAAATTGTGTATGTTAATGTGGCAGAAGCAACACCAAAGAGTATTATGAGGGTGATGGAAATTCCAGGACTTACTTTGTACCATCTCAAGAGCCATTTACAGGTATTCCCATTTTTGTAATGATATTGTAATTTTTTGGTTATTTTTGTTGACTGAAGAAATTGTGACTTTTCATGTTTTTTTACTGGTTGTAGAAATATAGACTTGGGAAAAGCCAACAACTAGAAACCTACTCTGACAACAAAGAACAAGGTGATGACTTGTAGGGAAACATATTTACTTCAGTAACTGCGTCGCACAGTTTTCAATCTCGACCATCTCGTATCAAAATAGTGGTCGAGATTGTTTATAGACTAATTGATTTTGTTATTGTGTAATTTGAGCCGTCTGATTTTTAAATTATCGGTCAAATTGTTTACAACGTGTAAATGTGTGTGTTTGTTAATCCAAATGATTGATTATTTGGCATAACATTGATGGTCCTTTTGGTGCAGTGTACACAGAAACCAAGAGCTGTGATGAACAATGCAGCAGAGAAATCAGTCTTGGGGACCAGAATCAAATCACTGAGTAATAATTAGAGCTTTTATCAAATATTTGATTATGATTTTTTCTACAAAATAATTTTAGATTGAACTGGTATTAGCTAGAGATTTTATTTTCAAATTCTGTTTTTGCCAGAAACATGGAGATATCTCATGCTCTAGAAATGCAAGTGGAAGTAGAAAGGAAACTTAATGAACAAATTGAGGTAACATAAATAATGCAATATCATGATTAAAATTTGCTTCAATCATAATTCTAACTTCCAAGTACATTTTTGGATTGGCGGTGAGTTCAGTAGAATCATGGTGTGCTACCGTGATTTTGGTAAAAAAAAAATACACTTTAAGCTGCAACAAAATCACGCTGCCACCGTGATTTCATCACATATACATTGTGTATCCAAATATGCACTAAATGTTGCATAAGGACGTGTTAATCAATTTGGTTGTTCATTTCAGGTGCAGAAACATTTGCAGCTTCGAATCGAAGCTCAAGGAAAATATTTACAATCGGTACTAATGAAGGCGCAGGAGGCCCTCTCTGGATACAATTCTTCACCAATTGGAATAAAACTGACAAAAGACGAACTGTCTCAATTGGTTACTATGATCAACAATGCATGTCCAAGTTCTCCAATCTCAGATCTAACAGAATCAAGAGGGTTAAGTTTAAACTATGAGGAGAGGAAACAGGACAATGGAACTTCTTTGTGTTCATTGGAAAGTTCTTTGACATCTTCTGAAATCTCAGAAAGGGAAGATAAACATTCAATAGAAGAAATAGGAGACTTCAAAAATTCTAGTGCTATTTCACTTGAATTGCCACTAATGGCCATGCACTCAGAAGAGAAATCATTGTCGTGTCCAATGTCTGTGTCTGTGCTTCGTAGCGATAATTTAAGTAATGAAGTCAATGGGAGAAAGAGAAATGAAGAAACTAAATTTGATGGTAGCTATGTTGAGAATTATTCATTAAGAAAAAGAGGTGGCAACAAGTTGAAGAAAGCTAAGTTGTCAGAAAAGTTTGACTTGAATAGCCAATATCAGAATGACAAGGACTCAACAAGTTCAAAAACGTTACTAGACTTAAATTGCAGTTTAAACTTTTGTGAACCATGA
Protein sequence

>MS.gene76602.t1

MQNQSMHFVLSTDAKPRLKWTPELHQRFIDAINQLGGAAKATPKSIMRVMEIPGLTLYHLKSHLQKYRLGKSQQLETYSDNKEQVYTETKSCDEQCSREISLGDQNQITENMEISHALEMQVEVERKLNEQIEVQKHLQLRIEAQGKYLQSVLMKAQEALSGYNSSPIGIKLTKDELSQLVTMINNACPSSPISDLTESRGLSLNYEERKQDNGTSLCSLESSLTSSEISEREDKHSIEEIGDFKNSSAISLELPLMAMHSEEKSLSCPMSVSVLRSDNLSNEVNGRKRNEETKFDGSYVENYSLRKRGGNKLKKAKLSEKFDLNSQYQNDKDSTSSKTLLDLNCSLNFCEP