AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76604

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76604.t1 MTR_5g041380 85.379 383 40 2 1 379 1 371 0.0 636
MS.gene76604.t1 MTR_5g041400 83.641 379 46 2 1 379 1 363 0.0 621
MS.gene76604.t1 MTR_5g041530 64.484 397 107 11 1 379 1 381 3.88e-149 427
MS.gene76604.t1 MTR_5g041420 61.697 389 72 9 1 379 1 322 4.50e-129 374
MS.gene76604.t1 MTR_4g082060 38.776 490 154 20 4 378 6 464 6.39e-75 240
MS.gene76604.t1 MTR_7g010950 33.602 497 193 17 2 377 4 484 1.20e-63 211
MS.gene76604.t1 MTR_6g012450 33.201 503 192 18 4 377 6 493 7.77e-53 182
MS.gene76604.t1 MTR_3g435480 36.508 378 128 14 101 377 97 463 5.02e-52 180
MS.gene76604.t1 MTR_8g044220 31.026 390 174 16 77 379 58 439 2.45e-39 145
MS.gene76604.t1 MTR_6g027460 58.586 99 35 2 96 188 51 149 6.11e-32 124
MS.gene76604.t1 MTR_2g016030 70.769 65 19 0 124 188 36 100 1.02e-29 112
MS.gene76604.t1 MTR_7g086780 29.428 367 155 12 103 379 70 422 2.14e-29 118
MS.gene76604.t1 MTR_6g027450 54.167 96 38 2 99 188 47 142 1.18e-27 111
MS.gene76604.t1 MTR_8g079060 63.492 63 23 0 135 197 29 91 5.76e-23 96.3
MS.gene76604.t1 MTR_4g022370 51.429 70 34 0 119 188 66 135 1.45e-21 95.1
MS.gene76604.t1 MTR_8g027295 56.250 64 28 0 125 188 14 77 3.56e-21 92.4
MS.gene76604.t1 MTR_2g093220 57.353 68 29 0 121 188 26 93 5.84e-21 92.4
MS.gene76604.t1 MTR_3g091820 49.398 83 38 1 109 191 21 99 7.62e-21 92.0
MS.gene76604.t1 MTR_8g479350 69.231 52 16 0 137 188 70 121 9.28e-21 92.4
MS.gene76604.t1 MTR_2g096740 67.308 52 17 0 137 188 64 115 9.65e-21 91.7
MS.gene76604.t1 MTR_2g096740 67.308 52 17 0 137 188 79 130 9.68e-21 92.0
MS.gene76604.t1 MTR_4g089095 64.151 53 19 0 137 189 52 104 1.42e-20 91.3
MS.gene76604.t1 MTR_4g089095 64.151 53 19 0 137 189 18 70 1.56e-20 90.5
MS.gene76604.t1 MTR_4g088580 71.154 52 15 0 137 188 57 108 1.67e-20 92.4
MS.gene76604.t1 MTR_1g077600 64.151 53 19 0 135 187 43 95 2.28e-20 90.1
MS.gene76604.t1 MTR_8g015840 67.308 52 17 0 135 186 20 71 2.33e-20 89.0
MS.gene76604.t1 MTR_4g109980 64.151 53 19 0 133 185 38 90 2.63e-20 90.9
MS.gene76604.t1 MTR_4g461080 61.538 65 21 1 127 187 17 81 2.66e-20 90.1
MS.gene76604.t1 MTR_1g077600 64.151 53 19 0 135 187 43 95 2.70e-20 90.5
MS.gene76604.t1 MTR_7g024670 68.627 51 16 0 137 187 79 129 2.91e-20 91.7
MS.gene76604.t1 MTR_1g056810 52.941 85 37 3 125 208 1 83 3.59e-20 90.1
MS.gene76604.t1 MTR_7g059400 51.429 70 34 0 119 188 60 129 4.31e-20 90.9
MS.gene76604.t1 MTR_4g063780 43.269 104 58 1 119 221 59 162 4.67e-20 90.5
MS.gene76604.t1 MTR_2g013370 68.519 54 16 1 135 188 46 98 5.34e-20 89.4
MS.gene76604.t1 MTR_8g068210 45.882 85 38 1 104 188 9 85 5.55e-20 90.1
MS.gene76604.t1 MTR_3g090430 63.158 57 20 1 133 188 48 104 1.41e-19 89.0
MS.gene76604.t1 MTR_2g059540 67.347 49 16 0 135 183 31 79 1.75e-19 86.3
MS.gene76604.t1 MTR_5g031440 60.000 55 22 0 131 185 39 93 1.31e-18 86.3
MS.gene76604.t1 MTR_2g014170 67.347 49 16 0 135 183 45 93 1.34e-18 85.9
MS.gene76604.t1 MTR_2g014060 66.000 50 17 0 137 186 27 76 1.95e-18 85.9
MS.gene76604.t1 MTR_3g077750 64.706 51 18 0 135 185 61 111 5.53e-18 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76604.t1 AT5G62430 44.599 287 133 9 106 379 25 298 2.25e-62 202
MS.gene76604.t1 AT3G47500 33.471 484 176 18 2 379 5 448 1.06e-52 181
MS.gene76604.t1 AT5G39660 35.616 365 147 13 93 377 99 455 5.65e-48 169
MS.gene76604.t1 AT5G39660 35.616 365 147 13 93 377 99 455 5.65e-48 169
MS.gene76604.t1 AT5G39660 35.616 365 147 13 93 377 99 455 5.65e-48 169
MS.gene76604.t1 AT1G69570 35.347 331 161 14 88 379 81 397 6.50e-44 157
MS.gene76604.t1 AT1G26790 34.062 320 170 11 90 379 64 372 1.42e-40 147
MS.gene76604.t1 AT1G29160 71.212 66 19 0 123 188 50 115 6.76e-32 119
MS.gene76604.t1 AT2G34140 55.914 93 36 2 101 188 19 111 1.81e-30 115
MS.gene76604.t1 AT3G55370 36.567 134 74 1 57 190 9 131 7.75e-24 100
MS.gene76604.t1 AT3G55370 36.567 134 74 1 57 190 9 131 1.36e-23 100
MS.gene76604.t1 AT1G51700 51.190 84 31 1 111 194 19 92 6.03e-22 92.8
MS.gene76604.t1 AT5G60200 57.143 70 30 0 125 194 45 114 7.93e-22 94.0
MS.gene76604.t1 AT3G55370 47.126 87 45 1 105 190 90 176 1.10e-21 95.5
MS.gene76604.t1 AT3G50410 71.154 52 15 0 135 186 30 81 1.30e-21 93.2
MS.gene76604.t1 AT3G61850 42.157 102 59 0 90 191 17 118 3.37e-21 92.8
MS.gene76604.t1 AT3G61850 42.157 102 59 0 90 191 17 118 3.37e-21 92.8
MS.gene76604.t1 AT3G21270 72.549 51 14 0 137 187 31 81 3.71e-21 90.9
MS.gene76604.t1 AT3G61850 42.157 102 59 0 90 191 29 130 3.85e-21 92.8
MS.gene76604.t1 AT3G61850 42.157 102 59 0 90 191 17 118 5.08e-21 92.8
MS.gene76604.t1 AT3G61850 42.157 102 59 0 90 191 29 130 5.38e-21 93.2
MS.gene76604.t1 AT2G28510 67.241 58 19 0 137 194 50 107 8.72e-21 91.7
MS.gene76604.t1 AT5G02460 52.857 70 33 0 119 188 79 148 1.04e-20 93.2
MS.gene76604.t1 AT5G60850 66.071 56 19 0 135 190 51 106 1.43e-20 91.7
MS.gene76604.t1 AT5G65590 49.425 87 41 1 104 190 13 96 1.48e-20 91.7
MS.gene76604.t1 AT5G66940 70.588 51 15 0 137 187 34 84 2.30e-20 89.4
MS.gene76604.t1 AT1G28310 66.667 54 18 0 135 188 27 80 3.81e-20 90.5
MS.gene76604.t1 AT4G24060 63.158 57 21 0 132 188 50 106 4.02e-20 90.9
MS.gene76604.t1 AT2G28810 52.239 67 31 1 121 186 62 128 4.14e-20 90.5
MS.gene76604.t1 AT1G07640 68.627 51 16 0 137 187 23 73 4.23e-20 89.7
MS.gene76604.t1 AT3G45610 59.016 61 25 0 131 191 36 96 4.31e-20 89.0
MS.gene76604.t1 AT1G28310 66.667 54 18 0 135 188 51 104 4.80e-20 90.5
MS.gene76604.t1 AT1G07640 68.627 51 16 0 137 187 79 129 4.90e-20 90.5
MS.gene76604.t1 AT2G37590 58.621 58 24 0 131 188 85 142 5.59e-20 90.1
MS.gene76604.t1 AT2G28810 52.239 67 31 1 121 186 79 145 5.61e-20 90.5
MS.gene76604.t1 AT1G07640 68.627 51 16 0 137 187 87 137 5.77e-20 90.5
MS.gene76604.t1 AT1G47655 69.388 49 15 0 135 183 29 77 1.41e-19 86.7
MS.gene76604.t1 AT4G00940 64.286 56 20 0 137 192 70 125 4.58e-19 87.0
MS.gene76604.t1 AT4G00940 64.286 56 20 0 137 192 70 125 4.58e-19 87.0
MS.gene76604.t1 AT4G00940 64.286 56 20 0 137 192 70 125 4.58e-19 87.0
MS.gene76604.t1 AT1G21340 49.398 83 39 1 118 200 21 100 5.63e-19 86.3
MS.gene76604.t1 AT4G38000 56.250 64 27 1 130 192 36 99 5.71e-19 85.9
MS.gene76604.t1 AT1G64620 51.471 68 29 1 116 183 34 97 6.40e-19 87.4
MS.gene76604.t1 AT3G52440 61.404 57 21 1 133 188 22 78 9.05e-19 85.1
MS.gene76604.t1 AT3G52440 61.404 57 21 1 133 188 41 97 1.23e-18 85.1
MS.gene76604.t1 AT5G62940 67.347 49 16 0 137 185 75 123 6.37e-18 84.7
MS.gene76604.t1 AT2G46590 59.615 52 21 0 131 182 64 115 2.69e-17 82.8
MS.gene76604.t1 AT2G46590 59.615 52 21 0 131 182 76 127 2.75e-17 82.8
MS.gene76604.t1 AT4G21050 50.725 69 32 1 120 188 11 77 3.86e-17 79.7
MS.gene76604.t1 AT4G21040 45.679 81 40 2 137 216 27 104 3.25e-15 74.7
MS.gene76604.t1 AT4G21080 55.769 52 23 0 137 188 27 78 1.56e-14 73.2

Find 69 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAATGGATTTGCATCTA+TGG 0.224783 5.1:+31224039 MS.gene76604:CDS
CTTGAATGTTTCCCTAAAAT+AGG 0.235391 5.1:-31223878 None:intergenic
AAACCCTTTGCATCTTCATT+AGG 0.263138 5.1:-31223977 None:intergenic
TCTTCAATTCTCAATTTCTT+AGG 0.264262 5.1:-31223953 None:intergenic
GCAAAAGCTGCCAGAGATAT+TGG 0.290195 5.1:+31223504 MS.gene76604:CDS
ACCACCATAGTATGCTATAT+TGG 0.313954 5.1:-31223795 None:intergenic
GGGAGTGTTTAAATCCTCTT+TGG 0.317886 5.1:-31223273 None:intergenic
AACCCTTTGCATCTTCATTA+GGG 0.318049 5.1:-31223976 None:intergenic
CTTGTCAAGAAAATCAATAT+TGG 0.352841 5.1:+31223677 MS.gene76604:CDS
TGCCAGAGATATTGGACTTC+CGG 0.368326 5.1:+31223512 MS.gene76604:CDS
ATGAGTAGTGTCTTGACCTT+TGG 0.369998 5.1:+31223623 MS.gene76604:CDS
AGTGAAGATGAAGACATAAT+AGG 0.382456 5.1:-31223656 None:intergenic
TTCCCTAAAATAGGTTTACT+TGG 0.385545 5.1:-31223869 None:intergenic
ACATGATTCAAAGAGATATA+AGG 0.401349 5.1:+31222781 MS.gene76604:CDS
CAACCCAATATAGCATACTA+TGG 0.408858 5.1:+31223791 MS.gene76604:CDS
CCACCATAGTATGCTATATT+GGG 0.415830 5.1:-31223794 None:intergenic
ATCAATTTCAGGAACAATCA+AGG 0.419402 5.1:+31223204 MS.gene76604:intron
CCTTGATAAAGCTGCAGGAT+TGG 0.440224 5.1:-31224110 None:intergenic
TTCAATGGATTTGCATCTAT+GGG 0.443267 5.1:+31224040 MS.gene76604:CDS
TTGCATCTATGGGTGGTGAT+AGG 0.444216 5.1:+31224050 MS.gene76604:CDS
ACACAGGATAACACATTGTT+GGG 0.445576 5.1:-31223763 None:intergenic
TAACACATTGTTGGGTTCCA+TGG 0.452271 5.1:-31223755 None:intergenic
GACACAGGATAACACATTGT+TGG 0.452547 5.1:-31223764 None:intergenic
CTCCGGAAGTCCAATATCTC+TGG 0.468706 5.1:-31223514 None:intergenic
CTCAATAAGGCTTGAAGATG+TGG 0.477034 5.1:-31223297 None:intergenic
CATACTTCTCGTGGTTCCTC+CGG 0.479008 5.1:-31223531 None:intergenic
GCAGAAGTGACGGGGCTGGT+TGG 0.486295 5.1:-31223483 None:intergenic
GAAGTTTCTGTTTCTGATGA+GGG 0.487694 5.1:-31223332 None:intergenic
TTTGAAGAATTCACTTCAAG+TGG 0.499476 5.1:-31222755 None:intergenic
GTGGTTGTTTAGTCCCATCA+TGG 0.499829 5.1:+31223813 MS.gene76604:CDS
TATATCTCTTTGAATCATGT+TGG 0.511854 5.1:-31222778 None:intergenic
TTCCCAATCAAATCCTCTCA+TGG 0.512251 5.1:-31224144 None:intergenic
AGAAGTTTCTGTTTCTGATG+AGG 0.515525 5.1:-31223333 None:intergenic
CCAATCCTGCAGCTTTATCA+AGG 0.521791 5.1:+31224110 MS.gene76604:CDS
TTAGGGAAACATTCAAGAGA+TGG 0.525491 5.1:+31223884 MS.gene76604:CDS
AGAAGTATGCTTGTAGGTGC+AGG 0.528984 5.1:+31223545 MS.gene76604:CDS
TAGGTTGTACGCTCCATGAT+GGG 0.544678 5.1:-31223826 None:intergenic
TGAGACCTTGATAAAGCTGC+AGG 0.554542 5.1:-31224115 None:intergenic
ATCGTAATCAAGTACTGAGG+TGG 0.554962 5.1:-31223234 None:intergenic
CATAGTATGCTATATTGGGT+TGG 0.557895 5.1:-31223790 None:intergenic
GACCCTAATGAAGATGCAAA+GGG 0.573223 5.1:+31223974 MS.gene76604:CDS
CTAGGAAACAAGAGTGGTGG+AGG 0.573390 5.1:+31224013 MS.gene76604:CDS
TTTGTTAGGAGCTTTGTCCT+GGG 0.573883 5.1:-31223396 None:intergenic
ACCACGAGAAGTATGCTTGT+AGG 0.578460 5.1:+31223539 MS.gene76604:CDS
GAGAAATCTTCATGAGCAAG+AGG 0.580785 5.1:-31222695 None:intergenic
ATAGGTTGTACGCTCCATGA+TGG 0.581466 5.1:-31223827 None:intergenic
AATAAGGCTTGAAGATGTGG+TGG 0.584261 5.1:-31223294 None:intergenic
GGGTTCCATGGAAACTGTTG+TGG 0.587185 5.1:-31223743 None:intergenic
AGACCCTAATGAAGATGCAA+AGG 0.590368 5.1:+31223973 MS.gene76604:CDS
GGTCTCATGTGTTCCATGAG+AGG 0.592053 5.1:+31224131 MS.gene76604:CDS
ACACAAGATTGAGTTGCGAT+AGG 0.596525 5.1:-31223845 None:intergenic
AATAGGAGAATCTGATCCAA+AGG 0.598047 5.1:-31223639 None:intergenic
TTGACGATTATGTGAGACAT+CGG 0.603312 5.1:-31223600 None:intergenic
ATTGGCTTTCAATACTGATG+AGG 0.608635 5.1:-31224092 None:intergenic
TCGTAATCAAGTACTGAGGT+GGG 0.612458 5.1:-31223233 None:intergenic
TGAACAAAGTGATATGTCCC+AGG 0.620522 5.1:+31223379 MS.gene76604:CDS
ATAAGGCTTGAAGATGTGGT+GGG 0.622284 5.1:-31223293 None:intergenic
AGCCTCAAAACAAACCAAAG+AGG 0.625789 5.1:+31223259 MS.gene76604:CDS
AATGGATTTGCATCTATGGG+TGG 0.631829 5.1:+31224043 MS.gene76604:CDS
ACCTACAAGCATACTTCTCG+TGG 0.650658 5.1:-31223540 None:intergenic
CCCAATATAGCATACTATGG+TGG 0.653975 5.1:+31223794 MS.gene76604:CDS
ACACTAGGAAACAAGAGTGG+TGG 0.656394 5.1:+31224010 MS.gene76604:CDS
AAATCTTCATGAGCAAGAGG+AGG 0.659290 5.1:-31222692 None:intergenic
ATATTGGGTTGGAATGACAC+AGG 0.664359 5.1:-31223779 None:intergenic
CAGAGATATTGGACTTCCGG+AGG 0.693631 5.1:+31223515 MS.gene76604:CDS
GGGTGGTGATAGGAATCATG+TGG 0.704890 5.1:+31224060 MS.gene76604:CDS
AAGAGGAGGAGAAAACTCAG+AGG 0.721417 5.1:-31222678 None:intergenic
TTCATCGTAATCAAGTACTG+AGG 0.724323 5.1:-31223237 None:intergenic
TTAACACTAGGAAACAAGAG+TGG 0.767808 5.1:+31224007 MS.gene76604:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATTACTTATAGTT+AGG + chr5.1:31222842-31222861 MS.gene76604:intron 10.0%
!! AATTATTGTTTATCAATTTC+AGG + chr5.1:31223193-31223212 MS.gene76604:intron 15.0%
!! TGATAAACAATAATTAGATT+AGG - chr5.1:31223188-31223207 None:intergenic 15.0%
!!! TGAAAAATATACAAAGTTTT+TGG + chr5.1:31223017-31223036 MS.gene76604:intron 15.0%
!! ATAGACTTCAAAATATGAAT+TGG - chr5.1:31223098-31223117 None:intergenic 20.0%
!!! AAGAAGAGATTTTATTCTTA+CGG - chr5.1:31223574-31223593 None:intergenic 20.0%
!!! TTTTGGTGATAAAATTCTTA+TGG + chr5.1:31222971-31222990 MS.gene76604:intron 20.0%
! ACATGATTCAAAGAGATATA+AGG + chr5.1:31222781-31222800 MS.gene76604:CDS 25.0%
! CTTGTCAAGAAAATCAATAT+TGG + chr5.1:31223677-31223696 MS.gene76604:CDS 25.0%
! TATATCTCTTTGAATCATGT+TGG - chr5.1:31222781-31222800 None:intergenic 25.0%
! TCTTCAATTCTCAATTTCTT+AGG - chr5.1:31223956-31223975 None:intergenic 25.0%
!! ACTTTGTTCATCAATTTTAG+TGG - chr5.1:31223369-31223388 None:intergenic 25.0%
!! ATTCAACCATTAAGCTTTTT+GGG + chr5.1:31222618-31222637 MS.gene76604:CDS 25.0%
!! TAACTTTGTGGTTCATATTT+TGG + chr5.1:31223157-31223176 MS.gene76604:intron 25.0%
!! TTCCAAGTAAACCTATTTTA+GGG + chr5.1:31223867-31223886 MS.gene76604:CDS 25.0%
AATTGCAATCAAACCAGTTT+AGG - chr5.1:31223124-31223143 None:intergenic 30.0%
AGTGAAGATGAAGACATAAT+AGG - chr5.1:31223659-31223678 None:intergenic 30.0%
ATCAATTTCAGGAACAATCA+AGG + chr5.1:31223204-31223223 MS.gene76604:intron 30.0%
CTTGAATGTTTCCCTAAAAT+AGG - chr5.1:31223881-31223900 None:intergenic 30.0%
TAAGTTTCATTGCAATCTTG+TGG + chr5.1:31222888-31222907 MS.gene76604:intron 30.0%
TGAAGTCTATAATCCTAAAC+TGG + chr5.1:31223108-31223127 MS.gene76604:intron 30.0%
TTCCCTAAAATAGGTTTACT+TGG - chr5.1:31223872-31223891 None:intergenic 30.0%
TTTGAAGAATTCACTTCAAG+TGG - chr5.1:31222758-31222777 None:intergenic 30.0%
! GATTCAACCATTAAGCTTTT+TGG + chr5.1:31222617-31222636 MS.gene76604:CDS 30.0%
! GTTCCAAGTAAACCTATTTT+AGG + chr5.1:31223866-31223885 MS.gene76604:CDS 30.0%
! TTCAATGGATTTGCATCTAT+GGG + chr5.1:31224040-31224059 MS.gene76604:CDS 30.0%
! TTTCAATGGATTTGCATCTA+TGG + chr5.1:31224039-31224058 MS.gene76604:CDS 30.0%
!! AAGATGCAAAGGGTTTTAAT+TGG + chr5.1:31223984-31224003 MS.gene76604:CDS 30.0%
!!! GGTTTTAATTGGTTAACACT+AGG + chr5.1:31223995-31224014 MS.gene76604:CDS 30.0%
!!! GTGCACTTTTTTCATCTTTT+TGG + chr5.1:31222954-31222973 MS.gene76604:intron 30.0%
AAACCCTTTGCATCTTCATT+AGG - chr5.1:31223980-31223999 None:intergenic 35.0%
AACCCTTTGCATCTTCATTA+GGG - chr5.1:31223979-31223998 None:intergenic 35.0%
AATAGGAGAATCTGATCCAA+AGG - chr5.1:31223642-31223661 None:intergenic 35.0%
ACACAGGATAACACATTGTT+GGG - chr5.1:31223766-31223785 None:intergenic 35.0%
ACCACCATAGTATGCTATAT+TGG - chr5.1:31223798-31223817 None:intergenic 35.0%
CAACCCAATATAGCATACTA+TGG + chr5.1:31223791-31223810 MS.gene76604:CDS 35.0%
CATAGTATGCTATATTGGGT+TGG - chr5.1:31223793-31223812 None:intergenic 35.0%
CCACCATAGTATGCTATATT+GGG - chr5.1:31223797-31223816 None:intergenic 35.0%
GCAACTTCAACTTAACTTTG+TGG + chr5.1:31223145-31223164 MS.gene76604:intron 35.0%
TGTTCTCCCAAAAAGCTTAA+TGG - chr5.1:31222627-31222646 None:intergenic 35.0%
TTAACACTAGGAAACAAGAG+TGG + chr5.1:31224007-31224026 MS.gene76604:CDS 35.0%
TTAGGGAAACATTCAAGAGA+TGG + chr5.1:31223884-31223903 MS.gene76604:CDS 35.0%
TTGACGATTATGTGAGACAT+CGG - chr5.1:31223603-31223622 None:intergenic 35.0%
! GGACATGGAACAATTTTGTT+AGG - chr5.1:31223413-31223432 None:intergenic 35.0%
! TTCATCGTAATCAAGTACTG+AGG - chr5.1:31223240-31223259 None:intergenic 35.0%
! TTTTTCCACAACAGTTTCCA+TGG + chr5.1:31223738-31223757 MS.gene76604:CDS 35.0%
!! AGAAGTTTCTGTTTCTGATG+AGG - chr5.1:31223336-31223355 None:intergenic 35.0%
!! ATTGGCTTTCAATACTGATG+AGG - chr5.1:31224095-31224114 None:intergenic 35.0%
!! GAAGTTTCTGTTTCTGATGA+GGG - chr5.1:31223335-31223354 None:intergenic 35.0%
!!! ATCCTCTTTGGTTTGTTTTG+AGG - chr5.1:31223264-31223283 None:intergenic 35.0%
AAATCTTCATGAGCAAGAGG+AGG - chr5.1:31222695-31222714 None:intergenic 40.0%
AATAAGGCTTGAAGATGTGG+TGG - chr5.1:31223297-31223316 None:intergenic 40.0%
AGACCCTAATGAAGATGCAA+AGG + chr5.1:31223973-31223992 MS.gene76604:CDS 40.0%
AGCCTCAAAACAAACCAAAG+AGG + chr5.1:31223259-31223278 MS.gene76604:CDS 40.0%
ATAAGGCTTGAAGATGTGGT+GGG - chr5.1:31223296-31223315 None:intergenic 40.0%
ATGAGTAGTGTCTTGACCTT+TGG + chr5.1:31223623-31223642 MS.gene76604:CDS 40.0%
CCCAATATAGCATACTATGG+TGG + chr5.1:31223794-31223813 MS.gene76604:CDS 40.0%
CTCAATAAGGCTTGAAGATG+TGG - chr5.1:31223300-31223319 None:intergenic 40.0%
GACACAGGATAACACATTGT+TGG - chr5.1:31223767-31223786 None:intergenic 40.0%
GACCCTAATGAAGATGCAAA+GGG + chr5.1:31223974-31223993 MS.gene76604:CDS 40.0%
GAGAAATCTTCATGAGCAAG+AGG - chr5.1:31222698-31222717 None:intergenic 40.0%
TAACACATTGTTGGGTTCCA+TGG - chr5.1:31223758-31223777 None:intergenic 40.0%
TGAACAAAGTGATATGTCCC+AGG + chr5.1:31223379-31223398 MS.gene76604:CDS 40.0%
! AATGGATTTGCATCTATGGG+TGG + chr5.1:31224043-31224062 MS.gene76604:CDS 40.0%
! ACACAAGATTGAGTTGCGAT+AGG - chr5.1:31223848-31223867 None:intergenic 40.0%
! TTTGTTAGGAGCTTTGTCCT+GGG - chr5.1:31223399-31223418 None:intergenic 40.0%
!! ATATTGGGTTGGAATGACAC+AGG - chr5.1:31223782-31223801 None:intergenic 40.0%
!! ATCGTAATCAAGTACTGAGG+TGG - chr5.1:31223237-31223256 None:intergenic 40.0%
!! GGGAGTGTTTAAATCCTCTT+TGG - chr5.1:31223276-31223295 None:intergenic 40.0%
!! TCGTAATCAAGTACTGAGGT+GGG - chr5.1:31223236-31223255 None:intergenic 40.0%
!! TTTTGTTAGGAGCTTTGTCC+TGG - chr5.1:31223400-31223419 None:intergenic 40.0%
!!! GTGTTTTCGGGTTCTCAATA+AGG - chr5.1:31223313-31223332 None:intergenic 40.0%
!!! GTTTCTGATGAGGGTGTTTT+CGG - chr5.1:31223326-31223345 None:intergenic 40.0%
!!! TTTCTGATGAGGGTGTTTTC+GGG - chr5.1:31223325-31223344 None:intergenic 40.0%
AAGAGGAGGAGAAAACTCAG+AGG - chr5.1:31222681-31222700 None:intergenic 45.0%
ACACTAGGAAACAAGAGTGG+TGG + chr5.1:31224010-31224029 MS.gene76604:CDS 45.0%
ACCTACAAGCATACTTCTCG+TGG - chr5.1:31223543-31223562 None:intergenic 45.0%
ATAGGTTGTACGCTCCATGA+TGG - chr5.1:31223830-31223849 None:intergenic 45.0%
CCTTGATAAAGCTGCAGGAT+TGG - chr5.1:31224113-31224132 None:intergenic 45.0%
GCAAAAGCTGCCAGAGATAT+TGG + chr5.1:31223504-31223523 MS.gene76604:CDS 45.0%
GTGGTTGTTTAGTCCCATCA+TGG + chr5.1:31223813-31223832 MS.gene76604:CDS 45.0%
TAGGTTGTACGCTCCATGAT+GGG - chr5.1:31223829-31223848 None:intergenic 45.0%
TGAGACCTTGATAAAGCTGC+AGG - chr5.1:31224118-31224137 None:intergenic 45.0%
TGCCAGAGATATTGGACTTC+CGG + chr5.1:31223512-31223531 MS.gene76604:CDS 45.0%
TTGCATCTATGGGTGGTGAT+AGG + chr5.1:31224050-31224069 MS.gene76604:CDS 45.0%
! ACCACGAGAAGTATGCTTGT+AGG + chr5.1:31223539-31223558 MS.gene76604:CDS 45.0%
! ACGCTTTTGCATCTTGGACA+TGG - chr5.1:31223428-31223447 None:intergenic 45.0%
! AGAAGTATGCTTGTAGGTGC+AGG + chr5.1:31223545-31223564 MS.gene76604:CDS 45.0%
! CCAATCCTGCAGCTTTATCA+AGG + chr5.1:31224110-31224129 MS.gene76604:CDS 45.0%
! GTGTCTACGCTTTTGCATCT+TGG - chr5.1:31223434-31223453 None:intergenic 45.0%
!! AGTGGTGGAGGACTTTTCAA+TGG + chr5.1:31224025-31224044 MS.gene76604:CDS 45.0%
CAGAGATATTGGACTTCCGG+AGG + chr5.1:31223515-31223534 MS.gene76604:CDS 50.0%
CATACTTCTCGTGGTTCCTC+CGG - chr5.1:31223534-31223553 None:intergenic 50.0%
CTAGGAAACAAGAGTGGTGG+AGG + chr5.1:31224013-31224032 MS.gene76604:CDS 50.0%
CTCCGGAAGTCCAATATCTC+TGG - chr5.1:31223517-31223536 None:intergenic 50.0%
GGGTGGTGATAGGAATCATG+TGG + chr5.1:31224060-31224079 MS.gene76604:CDS 50.0%
GGGTTCCATGGAAACTGTTG+TGG - chr5.1:31223746-31223765 None:intergenic 50.0%
GGTCTCATGTGTTCCATGAG+AGG + chr5.1:31224131-31224150 MS.gene76604:CDS 50.0%
! CAGCTTTTGCAGAAGTGACG+GGG - chr5.1:31223494-31223513 None:intergenic 50.0%
! GCAGCTTTTGCAGAAGTGAC+GGG - chr5.1:31223495-31223514 None:intergenic 50.0%
! GGCAGCTTTTGCAGAAGTGA+CGG - chr5.1:31223496-31223515 None:intergenic 50.0%
!! TTTTGCAGAAGTGACGGGGC+TGG - chr5.1:31223490-31223509 None:intergenic 55.0%
! GCAGAAGTGACGGGGCTGGT+TGG - chr5.1:31223486-31223505 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 31222608 31224159 31222608 ID=MS.gene76604
chr5.1 mRNA 31222608 31224159 31222608 ID=MS.gene76604.t1;Parent=MS.gene76604
chr5.1 exon 31222608 31222802 31222608 ID=MS.gene76604.t1.exon1;Parent=MS.gene76604.t1
chr5.1 CDS 31222608 31222802 31222608 ID=cds.MS.gene76604.t1;Parent=MS.gene76604.t1
chr5.1 exon 31223215 31224159 31223215 ID=MS.gene76604.t1.exon2;Parent=MS.gene76604.t1
chr5.1 CDS 31223215 31224159 31223215 ID=cds.MS.gene76604.t1;Parent=MS.gene76604.t1
Gene Sequence

>MS.gene76604

ATGACTAATGATTCAACCATTAAGCTTTTTGGGAGAACAATTTTTCAAACACACAACACTGATGATTGTTCCTCTGAGTTTTCTCCTCCTCTTGCTCATGAAGATTTCTCTGATCATAGTCTTCATTCATCTTTATCTTCATCTTCTCCACTTGAAGTGAATTCTTCAAACCAACATGATTCAAAGAGATATAAGGTATGTTTCATGCTAATTTTTCATCTTTTTTTTTGTTTTAAAATTAATTACTTATAGTTAGGTGTATGCAAATGTGTATTTAAACTAAGTTTCATTGCAATCTTGTGGCTATTTTGACAACTTTTTGCTTGAAAATATGCTTCATGAAATTGTGCACTTTTTTCATCTTTTTGGTGATAAAATTCTTATGGCTGTTTTGACAACTTTTTTTGCTTGAAAAATATACAAAGTTTTTGGATTTCATGATGACTGATGCTTGATGAAATTGTGCTTTTAAAGTTTTCAAATTTTTCCAATTCATATTTTGAAGTCTATAATCCTAAACTGGTTTGATTGCAATTTGCAACTTCAACTTAACTTTGTGGTTCATATTTTGGTCTTACCTAATCTAATTATTGTTTATCAATTTCAGGAACAATCAAGGAAAGAACCCACCTCAGTACTTGATTACGATGAAGCCTCAAAACAAACCAAAGAGGATTTAAACACTCCCACCACATCTTCAAGCCTTATTGAGAACCCGAAAACACCCTCATCAGAAACAGAAACTTCTCAACAGAATTCCACTAAAATTGATGAACAAAGTGATATGTCCCAGGACAAAGCTCCTAACAAAATTGTTCCATGTCCAAGATGCAAAAGCGTAGACACAAAATTTTGTTACTACAATAATTACAACGCCAACCAGCCCCGTCACTTCTGCAAAAGCTGCCAGAGATATTGGACTTCCGGAGGAACCACGAGAAGTATGCTTGTAGGTGCAGGTCGCCGTAAGAATAAAATCTCTTCTTTTTCTTCCGATGTCTCACATAATCGTCAAATGAGTAGTGTCTTGACCTTTGGATCAGATTCTCCTATTATGTCTTCATCTTCACTTGTCAAGAAAATCAATATTGGATCAAATGATGAAACATCTGATAAGAGTAATCAGTGTTTTTTTCCACAACAGTTTCCATGGAACCCAACAATGTGTTATCCTGTGTCATTCCAACCCAATATAGCATACTATGGTGGTTGTTTAGTCCCATCATGGAGCGTACAACCTATCGCAACTCAATCTTGTGTTCCAAGTAAACCTATTTTAGGGAAACATTCAAGAGATGGCTTAGAAAATAACAACAAAGAAAGTGATAATAATAGTGTGTTGATTCCTAAGAAATTGAGAATTGAAGACCCTAATGAAGATGCAAAGGGTTTTAATTGGTTAACACTAGGAAACAAGAGTGGTGGAGGACTTTTCAATGGATTTGCATCTATGGGTGGTGATAGGAATCATGTGGTTGAAGCAGCCTCATCAGTATTGAAAGCCAATCCTGCAGCTTTATCAAGGTCTCATGTGTTCCATGAGAGGATTTGA
Protein sequence

>MS.gene76604.t1

MTNDSTIKLFGRTIFQTHNTDDCSSEFSPPLAHEDFSDHSLHSSLSSSSPLEVNSSNQHDSKRYKEQSRKEPTSVLDYDEASKQTKEDLNTPTTSSSLIENPKTPSSETETSQQNSTKIDEQSDMSQDKAPNKIVPCPRCKSVDTKFCYYNNYNANQPRHFCKSCQRYWTSGGTTRSMLVGAGRRKNKISSFSSDVSHNRQMSSVLTFGSDSPIMSSSSLVKKINIGSNDETSDKSNQCFFPQQFPWNPTMCYPVSFQPNIAYYGGCLVPSWSVQPIATQSCVPSKPILGKHSRDGLENNNKESDNNSVLIPKKLRIEDPNEDAKGFNWLTLGNKSGGGLFNGFASMGGDRNHVVEAASSVLKANPAALSRSHVFHERI