AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76605

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76605.t1 MTR_5g041400 89.835 364 34 2 1 362 1 363 0.0 640
MS.gene76605.t1 MTR_5g041380 87.062 371 39 2 1 362 1 371 0.0 628
MS.gene76605.t1 MTR_5g041530 62.879 396 98 13 1 362 1 381 3.36e-140 403
MS.gene76605.t1 MTR_5g041420 57.216 388 74 10 1 362 1 322 3.29e-113 332
MS.gene76605.t1 MTR_4g082060 36.688 477 165 18 4 361 6 464 6.98e-68 221
MS.gene76605.t1 MTR_7g010950 35.010 497 169 20 2 360 4 484 4.07e-65 214
MS.gene76605.t1 MTR_3g435480 39.674 368 115 16 89 360 107 463 2.07e-55 188
MS.gene76605.t1 MTR_8g044220 31.767 447 198 19 4 362 12 439 1.73e-45 161
MS.gene76605.t1 MTR_6g012450 54.962 131 37 5 74 196 97 213 1.99e-34 132
MS.gene76605.t1 MTR_6g012450 40.000 165 61 8 222 360 341 493 1.31e-16 81.3
MS.gene76605.t1 MTR_7g086780 31.965 341 131 13 112 362 93 422 7.19e-33 127
MS.gene76605.t1 MTR_6g027460 60.000 90 30 2 88 171 60 149 4.53e-30 118
MS.gene76605.t1 MTR_2g016030 72.308 65 18 0 107 171 36 100 1.01e-29 112
MS.gene76605.t1 MTR_6g027450 55.556 90 34 2 88 171 53 142 1.84e-27 110
MS.gene76605.t1 MTR_8g079060 71.698 53 15 0 118 170 29 81 3.30e-22 94.0
MS.gene76605.t1 MTR_4g022370 48.718 78 40 0 94 171 58 135 2.03e-21 94.4
MS.gene76605.t1 MTR_4g109980 66.038 53 18 0 116 168 38 90 8.04e-21 92.0
MS.gene76605.t1 MTR_8g015840 69.231 52 16 0 118 169 20 71 8.27e-21 90.1
MS.gene76605.t1 MTR_8g479350 60.317 63 25 0 118 180 68 130 1.23e-20 92.0
MS.gene76605.t1 MTR_2g096740 64.815 54 19 0 118 171 62 115 2.80e-20 90.1
MS.gene76605.t1 MTR_2g096740 64.815 54 19 0 118 171 77 130 2.93e-20 90.1
MS.gene76605.t1 MTR_2g059540 69.388 49 15 0 118 166 31 79 3.33e-20 88.2
MS.gene76605.t1 MTR_3g091820 60.000 60 24 0 115 174 40 99 3.58e-20 90.1
MS.gene76605.t1 MTR_8g027295 54.688 64 29 0 108 171 14 77 4.01e-20 89.4
MS.gene76605.t1 MTR_2g093220 63.793 58 21 0 114 171 36 93 4.66e-20 89.4
MS.gene76605.t1 MTR_7g059400 69.231 52 16 0 120 171 78 129 6.27e-20 90.1
MS.gene76605.t1 MTR_4g063780 45.631 103 55 1 102 203 59 161 6.30e-20 89.7
MS.gene76605.t1 MTR_4g089095 61.818 55 21 0 118 172 50 104 8.03e-20 89.0
MS.gene76605.t1 MTR_4g089095 61.818 55 21 0 118 172 16 70 1.00e-19 88.2
MS.gene76605.t1 MTR_2g014170 48.148 81 41 1 87 166 13 93 1.28e-19 88.6
MS.gene76605.t1 MTR_1g077600 66.667 51 17 0 120 170 45 95 1.49e-19 87.8
MS.gene76605.t1 MTR_1g077600 66.667 51 17 0 120 170 45 95 1.53e-19 88.2
MS.gene76605.t1 MTR_4g461080 60.000 65 22 1 110 170 17 81 1.87e-19 87.4
MS.gene76605.t1 MTR_4g088580 69.231 52 16 0 120 171 57 108 2.09e-19 89.0
MS.gene76605.t1 MTR_7g024670 66.667 51 17 0 120 170 79 129 2.93e-19 88.6
MS.gene76605.t1 MTR_8g068210 45.263 95 41 3 100 191 22 108 6.92e-19 87.0
MS.gene76605.t1 MTR_3g090430 61.017 59 19 1 113 171 50 104 9.07e-19 86.7
MS.gene76605.t1 MTR_2g013370 67.308 52 16 1 120 171 48 98 1.16e-18 85.1
MS.gene76605.t1 MTR_1g056810 62.069 58 21 1 115 171 8 65 1.86e-18 84.7
MS.gene76605.t1 MTR_5g031440 58.182 55 23 0 114 168 39 93 7.12e-18 84.0
MS.gene76605.t1 MTR_2g014060 64.000 50 18 0 120 169 27 76 1.29e-17 83.2
MS.gene76605.t1 MTR_3g077750 64.706 51 18 0 118 168 61 111 2.16e-17 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76605.t1 AT5G62430 46.341 287 124 8 91 362 27 298 3.78e-65 209
MS.gene76605.t1 AT5G39660 38.727 377 133 16 74 360 87 455 6.10e-52 179
MS.gene76605.t1 AT5G39660 38.727 377 133 16 74 360 87 455 6.10e-52 179
MS.gene76605.t1 AT5G39660 38.727 377 133 16 74 360 87 455 6.10e-52 179
MS.gene76605.t1 AT1G69570 36.637 333 142 16 83 362 81 397 1.80e-46 163
MS.gene76605.t1 AT1G26790 35.274 292 150 10 99 362 92 372 5.72e-43 153
MS.gene76605.t1 AT1G29160 74.242 66 17 0 106 171 50 115 1.28e-32 120
MS.gene76605.t1 AT3G47500 57.658 111 41 2 77 186 68 173 1.46e-31 124
MS.gene76605.t1 AT3G47500 42.308 156 73 5 224 362 293 448 3.30e-20 92.0
MS.gene76605.t1 AT2G34140 47.059 119 52 2 81 188 15 133 2.34e-31 117
MS.gene76605.t1 AT3G55370 50.575 87 43 0 87 173 45 131 2.61e-23 99.4
MS.gene76605.t1 AT3G55370 50.575 87 43 0 87 173 90 176 2.91e-23 99.8
MS.gene76605.t1 AT3G55370 50.575 87 43 0 87 173 45 131 3.25e-23 99.4
MS.gene76605.t1 AT1G51700 62.500 64 24 0 118 181 33 96 4.75e-22 92.8
MS.gene76605.t1 AT2G28810 42.268 97 56 0 73 169 32 128 1.04e-21 94.7
MS.gene76605.t1 AT2G28810 42.268 97 56 0 73 169 49 145 1.27e-21 94.7
MS.gene76605.t1 AT5G60200 54.795 73 33 0 108 180 45 117 1.77e-21 92.8
MS.gene76605.t1 AT5G60850 67.857 56 18 0 118 173 51 106 2.84e-21 93.2
MS.gene76605.t1 AT3G50410 71.154 52 15 0 118 169 30 81 4.84e-21 91.7
MS.gene76605.t1 AT5G02460 55.714 70 31 0 102 171 79 148 1.29e-20 92.8
MS.gene76605.t1 AT2G37590 62.069 58 22 0 114 171 85 142 1.92e-20 91.3
MS.gene76605.t1 AT3G21270 67.925 53 17 0 118 170 29 81 2.45e-20 88.6
MS.gene76605.t1 AT5G66940 66.038 53 18 0 118 170 32 84 6.37e-20 87.8
MS.gene76605.t1 AT1G07640 56.716 67 26 1 104 170 10 73 8.54e-20 88.6
MS.gene76605.t1 AT2G28510 54.286 70 32 0 108 177 38 107 9.56e-20 88.6
MS.gene76605.t1 AT5G65590 54.054 74 31 1 100 173 26 96 1.10e-19 89.0
MS.gene76605.t1 AT1G07640 56.716 67 26 1 104 170 66 129 1.16e-19 89.0
MS.gene76605.t1 AT1G07640 56.716 67 26 1 104 170 74 137 1.37e-19 89.0
MS.gene76605.t1 AT3G45610 57.377 61 26 0 114 174 36 96 1.56e-19 87.0
MS.gene76605.t1 AT3G61850 59.016 61 25 0 114 174 58 118 1.98e-19 87.8
MS.gene76605.t1 AT3G61850 59.016 61 25 0 114 174 58 118 1.98e-19 87.8
MS.gene76605.t1 AT3G61850 59.016 61 25 0 114 174 70 130 2.03e-19 87.8
MS.gene76605.t1 AT1G28310 66.667 54 18 0 118 171 27 80 2.15e-19 88.2
MS.gene76605.t1 AT4G38000 56.250 64 27 1 113 175 36 99 2.34e-19 86.7
MS.gene76605.t1 AT3G61850 59.016 61 25 0 114 174 58 118 2.78e-19 87.8
MS.gene76605.t1 AT3G61850 59.016 61 25 0 114 174 70 130 3.02e-19 87.8
MS.gene76605.t1 AT1G28310 66.667 54 18 0 118 171 51 104 3.06e-19 87.8
MS.gene76605.t1 AT1G64620 52.941 68 28 1 99 166 34 97 3.26e-19 88.2
MS.gene76605.t1 AT1G47655 51.948 77 33 2 92 166 3 77 4.27e-19 85.1
MS.gene76605.t1 AT4G24060 59.677 62 21 1 110 171 49 106 4.63e-19 87.4
MS.gene76605.t1 AT3G52440 47.727 88 42 2 120 207 27 110 3.71e-18 83.2
MS.gene76605.t1 AT1G21340 45.977 87 44 1 97 183 17 100 3.86e-18 83.6
MS.gene76605.t1 AT3G52440 46.591 88 43 1 120 207 46 129 6.39e-18 83.2
MS.gene76605.t1 AT4G00940 60.714 56 22 0 120 175 70 125 7.62e-18 83.2
MS.gene76605.t1 AT4G00940 60.714 56 22 0 120 175 70 125 7.62e-18 83.2
MS.gene76605.t1 AT4G00940 60.714 56 22 0 120 175 70 125 7.62e-18 83.2
MS.gene76605.t1 AT4G21050 45.455 88 46 1 103 190 9 94 1.73e-17 80.5
MS.gene76605.t1 AT2G46590 55.932 59 25 1 108 165 57 115 2.89e-17 82.4
MS.gene76605.t1 AT2G46590 55.932 59 25 1 108 165 69 127 3.24e-17 82.4
MS.gene76605.t1 AT5G62940 65.306 49 17 0 120 168 75 123 3.86e-17 82.4
MS.gene76605.t1 AT4G21040 55.769 52 23 0 120 171 27 78 4.35e-15 74.3
MS.gene76605.t1 AT4G21080 55.769 52 23 0 120 171 27 78 1.45e-14 73.2

Find 71 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAATGGATTTGCATCTA+TGG 0.198741 5.1:+31228948 MS.gene76605:CDS
GTTCCAAGTAAACCTACTTT+AGG 0.234419 5.1:+31228775 MS.gene76605:CDS
TCTTCAATTCTTAATTTCTT+AGG 0.243331 5.1:-31228862 None:intergenic
GAACCCTTTGCAGCTTCATT+AGG 0.275268 5.1:-31228886 None:intergenic
GAGGTAGGTTCTTTCCTTGA+TGG 0.282122 5.1:-31228163 None:intergenic
CAACCCAATATAGCATACTA+TGG 0.283526 5.1:+31228700 MS.gene76605:CDS
AAGCTGCAAAGGGTTCTAAT+TGG 0.333760 5.1:+31228893 MS.gene76605:CDS
ATGAGTACTGTCTTGAACTT+TGG 0.343501 5.1:+31228532 MS.gene76605:CDS
AACCCTTTGCAGCTTCATTA+GGG 0.351302 5.1:-31228885 None:intergenic
TTCCAAGTAAACCTACTTTA+GGG 0.369270 5.1:+31228776 MS.gene76605:CDS
TTCCCTAAAGTAGGTTTACT+TGG 0.378626 5.1:-31228778 None:intergenic
AGTGAAGTTGAAGACATAAT+AGG 0.397363 5.1:-31228565 None:intergenic
GAATTCTTCAAAGAGATATA+AGG 0.418199 5.1:+31227592 MS.gene76605:CDS
GGGGTTGTTTGGTCCCATCA+TGG 0.418852 5.1:+31228722 MS.gene76605:CDS
CCTTGATAAAGCTGCAGGAT+TGG 0.440224 5.1:-31229019 None:intergenic
TTTGAAGAATTCACTTCAAG+TGG 0.441472 5.1:-31227581 None:intergenic
ACTTTGTTCATCAACTTTCG+TGG 0.442217 5.1:-31228275 None:intergenic
TTCAATGGATTTGCATCTAT+GGG 0.445750 5.1:+31228949 MS.gene76605:CDS
GAAACAGGATAACACATTGC+TGG 0.451292 5.1:-31228673 None:intergenic
ATATTGGGTTGGAATGAAAC+AGG 0.454445 5.1:-31228688 None:intergenic
CTTGAATGTTTCCCTAAAGT+AGG 0.465584 5.1:-31228787 None:intergenic
ATCAATTTCAGAAACCATCA+AGG 0.479118 5.1:+31228149 MS.gene76605:intron
GGATCAGAAGGTGAAACATC+TGG 0.482490 5.1:+31228607 MS.gene76605:CDS
CATACTTCTCGTGGTTCCTC+CGG 0.484155 5.1:-31228440 None:intergenic
GGTTCTAATTGGTTAACACT+TGG 0.486031 5.1:+31228904 MS.gene76605:CDS
TAACACATTGCTGGGTTCCA+TGG 0.491823 5.1:-31228664 None:intergenic
AACCCAATATAGCATACTAT+GGG 0.495182 5.1:+31228701 MS.gene76605:CDS
AAAATGAATGTTGGATCAGA+AGG 0.495845 5.1:+31228595 MS.gene76605:CDS
CTCGACAAGAAAATGAATGT+TGG 0.500402 5.1:+31228586 MS.gene76605:CDS
GCAGAAGTGACGGGGCTGGT+TGG 0.505366 5.1:-31228392 None:intergenic
TTCCCAATCAAATCCTCTCA+TGG 0.512251 5.1:-31229053 None:intergenic
AGTTTCTGTTTCTGATGTGG+TGG 0.517750 5.1:-31228239 None:intergenic
CCAATCCTGCAGCTTTATCA+AGG 0.521791 5.1:+31229019 MS.gene76605:CDS
AGAAGTTTCTGTTTCTGATG+TGG 0.528428 5.1:-31228242 None:intergenic
TTAGGGAAACATTCAAGAGA+TGG 0.534355 5.1:+31228793 MS.gene76605:CDS
TTTGTTTGGAGCTTTGTCCT+GGG 0.536610 5.1:-31228305 None:intergenic
AAAGACCTTGATAAAGCTGC+AGG 0.537292 5.1:-31229024 None:intergenic
TGTCAAAGATATTGGACTGC+CGG 0.537892 5.1:+31228421 MS.gene76605:CDS
GACCCTAATGAAGCTGCAAA+GGG 0.538987 5.1:+31228883 MS.gene76605:CDS
TAGGTTGTACGCTCCATGAT+GGG 0.539001 5.1:-31228735 None:intergenic
TTGACGATTATGTGAGGCAT+CGG 0.539506 5.1:-31228509 None:intergenic
AAACAGGATAACACATTGCT+GGG 0.549807 5.1:-31228672 None:intergenic
TTAACACTTGGAATCAAAAG+TGG 0.550116 5.1:+31228916 MS.gene76605:CDS
TCATAATCAAGTACTGAGGT+AGG 0.557713 5.1:-31228178 None:intergenic
CATAGTATGCTATATTGGGT+TGG 0.557895 5.1:-31228699 None:intergenic
AGAAGTATGCTTGTAGGCGC+AGG 0.559031 5.1:+31228454 MS.gene76605:CDS
GTTTCTGTTTCTGATGTGGT+GGG 0.561237 5.1:-31228238 None:intergenic
GTGTTGAAATCCTCTGTGGT+GGG 0.563237 5.1:-31228214 None:intergenic
ACCACGAGAAGTATGCTTGT+AGG 0.566681 5.1:+31228448 MS.gene76605:CDS
AGACCCTAATGAAGCTGCAA+AGG 0.568759 5.1:+31228882 MS.gene76605:CDS
AGTGTTGAAATCCTCTGTGG+TGG 0.573158 5.1:-31228215 None:intergenic
GAGAAATCTTCATGAGCAAG+AGG 0.580785 5.1:-31227521 None:intergenic
ATAGGTTGTACGCTCCATGA+TGG 0.581466 5.1:-31228736 None:intergenic
TGAACAAAGTGATATTTCCC+AGG 0.582852 5.1:+31228288 MS.gene76605:CDS
AAACTCAGAGGAACAATCAT+CGG 0.585405 5.1:-31227492 None:intergenic
GGGTTCCATGGAAACTGTTG+TGG 0.589831 5.1:-31228652 None:intergenic
ACACAAGATTGAGTTGCGAT+AGG 0.596525 5.1:-31228754 None:intergenic
ATTGGCTTTCAATACTGATG+AGG 0.608635 5.1:-31229001 None:intergenic
GCCTACAAGCATACTTCTCG+TGG 0.629329 5.1:-31228449 None:intergenic
AATGGATTTGCATCTATGGG+TGG 0.631829 5.1:+31228952 MS.gene76605:CDS
ACACTTGGAATCAAAAGTGG+TGG 0.634859 5.1:+31228919 MS.gene76605:CDS
CAAAGATATTGGACTGCCGG+AGG 0.649186 5.1:+31228424 MS.gene76605:CDS
AAATCTTCATGAGCAAGAGG+AGG 0.659290 5.1:-31227518 None:intergenic
ACCCAATATAGCATACTATG+GGG 0.674775 5.1:+31228702 MS.gene76605:CDS
ACTCATTTGACGATTATGTG+AGG 0.678683 5.1:-31228515 None:intergenic
CCCAATATAGCATACTATGG+GGG 0.689551 5.1:+31228703 MS.gene76605:CDS
TTCTTCATAATCAAGTACTG+AGG 0.698198 5.1:-31228182 None:intergenic
GGGAGTGTTGAAATCCTCTG+TGG 0.719304 5.1:-31228218 None:intergenic
GGGTGGTGATAAGAATCATG+TGG 0.719528 5.1:+31228969 MS.gene76605:CDS
AAGAGGAGGAGAAAACTCAG+AGG 0.721417 5.1:-31227504 None:intergenic
AGCCTCAAAACCCACCACAG+AGG 0.733290 5.1:+31228204 MS.gene76605:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTTATTGTGAATTATTAA+TGG - chr5.1:31227828-31227847 None:intergenic 15.0%
!!! AAATTTAATTACTTCTAGTT+AGG + chr5.1:31227650-31227669 MS.gene76605:intron 15.0%
!!! ACTTTGAGTTTATTTTTTAT+TGG + chr5.1:31227998-31228017 MS.gene76605:intron 15.0%
!!! ATGAAAAATATTTAACTTTG+TGG + chr5.1:31228090-31228109 MS.gene76605:intron 15.0%
!!! TGAAATATATACAATGTTTT+TGG + chr5.1:31227713-31227732 MS.gene76605:intron 15.0%
!! CTTTAAGTAATTGTGATTAA+AGG + chr5.1:31227858-31227877 MS.gene76605:intron 20.0%
!! GAATAAAAAATAATATGAGC+TGG - chr5.1:31227927-31227946 None:intergenic 20.0%
!! TCTTCAATTCTTAATTTCTT+AGG - chr5.1:31228865-31228884 None:intergenic 20.0%
!! TGATAAACAATGATTAGATT+AGG - chr5.1:31228133-31228152 None:intergenic 20.0%
!! TTAAATAAAAAGTTAAGAGC+TGG - chr5.1:31228034-31228053 None:intergenic 20.0%
!!! TATTTTTTATTGGATCTCAT+AGG + chr5.1:31228008-31228027 MS.gene76605:intron 20.0%
! GAATTCTTCAAAGAGATATA+AGG + chr5.1:31227592-31227611 MS.gene76605:CDS 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5.1:31227444-31227463 MS.gene76605:CDS 25.0%
AAAATGAATGTTGGATCAGA+AGG + chr5.1:31228595-31228614 MS.gene76605:CDS 30.0%
AACCCAATATAGCATACTAT+GGG + chr5.1:31228701-31228720 MS.gene76605:CDS 30.0%
AGTGAAGTTGAAGACATAAT+AGG - chr5.1:31228568-31228587 None:intergenic 30.0%
ATCAATTTCAGAAACCATCA+AGG + chr5.1:31228149-31228168 MS.gene76605:intron 30.0%
GCAAAAACTGTCAAAGATAT+TGG + chr5.1:31228413-31228432 MS.gene76605:CDS 30.0%
TGAAGTCTATAATCCTAAAC+TGG + chr5.1:31227953-31227972 MS.gene76605:intron 30.0%
TTAACACTTGGAATCAAAAG+TGG + chr5.1:31228916-31228935 MS.gene76605:CDS 30.0%
TTCCAAGTAAACCTACTTTA+GGG + chr5.1:31228776-31228795 MS.gene76605:CDS 30.0%
TTTCAAGAAAGACCAATAGT+AGG + chr5.1:31227901-31227920 MS.gene76605:intron 30.0%
TTTGAAGAATTCACTTCAAG+TGG - chr5.1:31227584-31227603 None:intergenic 30.0%
! GATTCAACCATAAAGCTTTT+TGG + chr5.1:31227443-31227462 MS.gene76605:CDS 30.0%
! TAACTTTGTGGTTCATCTTT+TGG + chr5.1:31228102-31228121 MS.gene76605:intron 30.0%
! TTCAATGGATTTGCATCTAT+GGG + chr5.1:31228949-31228968 MS.gene76605:CDS 30.0%
! TTCTTCATAATCAAGTACTG+AGG - chr5.1:31228185-31228204 None:intergenic 30.0%
! TTTCAATGGATTTGCATCTA+TGG + chr5.1:31228948-31228967 MS.gene76605:CDS 30.0%
AAACAGGATAACACATTGCT+GGG - chr5.1:31228675-31228694 None:intergenic 35.0%
AAACTCAGAGGAACAATCAT+CGG - chr5.1:31227495-31227514 None:intergenic 35.0%
ACCCAATATAGCATACTATG+GGG + chr5.1:31228702-31228721 MS.gene76605:CDS 35.0%
ACTCATTTGACGATTATGTG+AGG - chr5.1:31228518-31228537 None:intergenic 35.0%
ACTTTGTTCATCAACTTTCG+TGG - chr5.1:31228278-31228297 None:intergenic 35.0%
CAACCCAATATAGCATACTA+TGG + chr5.1:31228700-31228719 MS.gene76605:CDS 35.0%
CATAGTATGCTATATTGGGT+TGG - chr5.1:31228702-31228721 None:intergenic 35.0%
CTCGACAAGAAAATGAATGT+TGG + chr5.1:31228586-31228605 MS.gene76605:CDS 35.0%
CTTGAATGTTTCCCTAAAGT+AGG - chr5.1:31228790-31228809 None:intergenic 35.0%
GTTCCAAGTAAACCTACTTT+AGG + chr5.1:31228775-31228794 MS.gene76605:CDS 35.0%
GTTGCAAATCAAACCAGTTT+AGG - chr5.1:31227969-31227988 None:intergenic 35.0%
TGAACAAAGTGATATTTCCC+AGG + chr5.1:31228288-31228307 MS.gene76605:CDS 35.0%
TTAGGGAAACATTCAAGAGA+TGG + chr5.1:31228793-31228812 MS.gene76605:CDS 35.0%
TTCCCTAAAGTAGGTTTACT+TGG - chr5.1:31228781-31228800 None:intergenic 35.0%
! ATGAGTACTGTCTTGAACTT+TGG + chr5.1:31228532-31228551 MS.gene76605:CDS 35.0%
! GGACATGGAAGAATTTTGTT+TGG - chr5.1:31228322-31228341 None:intergenic 35.0%
! GGTGTTGTGTGTTTGAAAAA+TGG - chr5.1:31227474-31227493 None:intergenic 35.0%
! GGTTCTAATTGGTTAACACT+TGG + chr5.1:31228904-31228923 MS.gene76605:CDS 35.0%
! TTTTTCCACAACAGTTTCCA+TGG + chr5.1:31228647-31228666 MS.gene76605:CDS 35.0%
!! AGAAGTTTCTGTTTCTGATG+TGG - chr5.1:31228245-31228264 None:intergenic 35.0%
!! ATATTGGGTTGGAATGAAAC+AGG - chr5.1:31228691-31228710 None:intergenic 35.0%
!! ATTGGCTTTCAATACTGATG+AGG - chr5.1:31229004-31229023 None:intergenic 35.0%
!! TCATAATCAAGTACTGAGGT+AGG - chr5.1:31228181-31228200 None:intergenic 35.0%
AAAGACCTTGATAAAGCTGC+AGG - chr5.1:31229027-31229046 None:intergenic 40.0%
AAATCTTCATGAGCAAGAGG+AGG - chr5.1:31227521-31227540 None:intergenic 40.0%
AACCCTTTGCAGCTTCATTA+GGG - chr5.1:31228888-31228907 None:intergenic 40.0%
ACACTTGGAATCAAAAGTGG+TGG + chr5.1:31228919-31228938 MS.gene76605:CDS 40.0%
ACCCCCATAGTATGCTATAT+TGG - chr5.1:31228707-31228726 None:intergenic 40.0%
CCCAATATAGCATACTATGG+GGG + chr5.1:31228703-31228722 MS.gene76605:CDS 40.0%
CCCCCATAGTATGCTATATT+GGG - chr5.1:31228706-31228725 None:intergenic 40.0%
GAAACAGGATAACACATTGC+TGG - chr5.1:31228676-31228695 None:intergenic 40.0%
GAGAAATCTTCATGAGCAAG+AGG - chr5.1:31227524-31227543 None:intergenic 40.0%
GGTTCTCCCAAAAAGCTTTA+TGG - chr5.1:31227453-31227472 None:intergenic 40.0%
GTTAGGTGTATGCAATCTTG+TGG + chr5.1:31227667-31227686 MS.gene76605:intron 40.0%
TGTCAAAGATATTGGACTGC+CGG + chr5.1:31228421-31228440 MS.gene76605:CDS 40.0%
TTGACGATTATGTGAGGCAT+CGG - chr5.1:31228512-31228531 None:intergenic 40.0%
! AAGCTGCAAAGGGTTCTAAT+TGG + chr5.1:31228893-31228912 MS.gene76605:CDS 40.0%
! AATATGAGCTGGCCTACTAT+TGG - chr5.1:31227916-31227935 None:intergenic 40.0%
! AATGGATTTGCATCTATGGG+TGG + chr5.1:31228952-31228971 MS.gene76605:CDS 40.0%
! ACACAAGATTGAGTTGCGAT+AGG - chr5.1:31228757-31228776 None:intergenic 40.0%
! ACAGTTTTTGCAGAAGTGAC+GGG - chr5.1:31228404-31228423 None:intergenic 40.0%
! ATGCTTTTGCATCTTGGACA+TGG - chr5.1:31228337-31228356 None:intergenic 40.0%
! GACAGTTTTTGCAGAAGTGA+CGG - chr5.1:31228405-31228424 None:intergenic 40.0%
! GTGTCTATGCTTTTGCATCT+TGG - chr5.1:31228343-31228362 None:intergenic 40.0%
! TTTGTTTGGAGCTTTGTCCT+GGG - chr5.1:31228308-31228327 None:intergenic 40.0%
!! AGTGGTGGAGCATTTTTCAA+TGG + chr5.1:31228934-31228953 MS.gene76605:CDS 40.0%
!! AGTTTCTGTTTCTGATGTGG+TGG - chr5.1:31228242-31228261 None:intergenic 40.0%
!! GTTTCTGTTTCTGATGTGGT+GGG - chr5.1:31228241-31228260 None:intergenic 40.0%
!! TTTTGTTTGGAGCTTTGTCC+TGG - chr5.1:31228309-31228328 None:intergenic 40.0%
AAGAGGAGGAGAAAACTCAG+AGG - chr5.1:31227507-31227526 None:intergenic 45.0%
AGACCCTAATGAAGCTGCAA+AGG + chr5.1:31228882-31228901 MS.gene76605:CDS 45.0%
AGCATACTATGGGGGTTGTT+TGG + chr5.1:31228711-31228730 MS.gene76605:CDS 45.0%
ATAGGTTGTACGCTCCATGA+TGG - chr5.1:31228739-31228758 None:intergenic 45.0%
CCTTGATAAAGCTGCAGGAT+TGG - chr5.1:31229022-31229041 None:intergenic 45.0%
GAACCCTTTGCAGCTTCATT+AGG - chr5.1:31228889-31228908 None:intergenic 45.0%
GACCCTAATGAAGCTGCAAA+GGG + chr5.1:31228883-31228902 MS.gene76605:CDS 45.0%
GAGGTAGGTTCTTTCCTTGA+TGG - chr5.1:31228166-31228185 None:intergenic 45.0%
GGATCAGAAGGTGAAACATC+TGG + chr5.1:31228607-31228626 MS.gene76605:CDS 45.0%
GGGTGGTGATAAGAATCATG+TGG + chr5.1:31228969-31228988 MS.gene76605:CDS 45.0%
GTGTTGAAATCCTCTGTGGT+GGG - chr5.1:31228217-31228236 None:intergenic 45.0%
TAACACATTGCTGGGTTCCA+TGG - chr5.1:31228667-31228686 None:intergenic 45.0%
TAGGTTGTACGCTCCATGAT+GGG - chr5.1:31228738-31228757 None:intergenic 45.0%
! ACCACGAGAAGTATGCTTGT+AGG + chr5.1:31228448-31228467 MS.gene76605:CDS 45.0%
! AGTGTTGAAATCCTCTGTGG+TGG - chr5.1:31228218-31228237 None:intergenic 45.0%
! CAGTTTTTGCAGAAGTGACG+GGG - chr5.1:31228403-31228422 None:intergenic 45.0%
! CCAATCCTGCAGCTTTATCA+AGG + chr5.1:31229019-31229038 MS.gene76605:CDS 45.0%
! GGTCTTTTGTGTTCCATGAG+AGG + chr5.1:31229040-31229059 MS.gene76605:CDS 45.0%
CAAAGATATTGGACTGCCGG+AGG + chr5.1:31228424-31228443 MS.gene76605:CDS 50.0%
CATACTTCTCGTGGTTCCTC+CGG - chr5.1:31228443-31228462 None:intergenic 50.0%
GCCTACAAGCATACTTCTCG+TGG - chr5.1:31228452-31228471 None:intergenic 50.0%
GGGTTCCATGGAAACTGTTG+TGG - chr5.1:31228655-31228674 None:intergenic 50.0%
! AGAAGTATGCTTGTAGGCGC+AGG + chr5.1:31228454-31228473 MS.gene76605:CDS 50.0%
!! GGGAGTGTTGAAATCCTCTG+TGG - chr5.1:31228221-31228240 None:intergenic 50.0%
!!! ATCCTCTGTGGTGGGTTTTG+AGG - chr5.1:31228209-31228228 None:intergenic 50.0%
AGCCTCAAAACCCACCACAG+AGG + chr5.1:31228204-31228223 MS.gene76605:CDS 55.0%
GGGGTTGTTTGGTCCCATCA+TGG + chr5.1:31228722-31228741 MS.gene76605:CDS 55.0%
!! TTTTGCAGAAGTGACGGGGC+TGG - chr5.1:31228399-31228418 None:intergenic 55.0%
! GCAGAAGTGACGGGGCTGGT+TGG - chr5.1:31228395-31228414 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 31227434 31229068 31227434 ID=MS.gene76605
chr5.1 mRNA 31227434 31229068 31227434 ID=MS.gene76605.t1;Parent=MS.gene76605
chr5.1 exon 31227434 31227613 31227434 ID=MS.gene76605.t1.exon1;Parent=MS.gene76605.t1
chr5.1 CDS 31227434 31227613 31227434 ID=cds.MS.gene76605.t1;Parent=MS.gene76605.t1
chr5.1 exon 31228160 31229068 31228160 ID=MS.gene76605.t1.exon2;Parent=MS.gene76605.t1
chr5.1 CDS 31228160 31229068 31228160 ID=cds.MS.gene76605.t1;Parent=MS.gene76605.t1
Gene Sequence

>MS.gene76605

ATGACTAATGATTCAACCATAAAGCTTTTTGGGAGAACCATTTTTCAAACACACAACACCGATGATTGTTCCTCTGAGTTTTCTCCTCCTCTTGCTCATGAAGATTTCTCTGATCATAGTCTTCATTCATCTTTATCTTCATCTTCTCCACTTGAAGTGAATTCTTCAAAGAGATATAAGGTATGTTTCATGCAATTTTTTCATCTTTTTTGTTTTAAATTTAATTACTTCTAGTTAGGTGTATGCAATCTTGTGGCTGTTTTGACAACTTTTTTTGCTTGAAATATATACAATGTTTTTGGATTTCATGATGACAGATGCTTGATGAAATTGTGCACTTTTTTCATTTGTATATTTATTTAAAAGTTTAGTTTTGATAGTGTCTGTAAAACCATTAATAATTCACAATAAATCATATGTACAACTTTAAGTAATTGTGATTAAAGGAACTTAATCAAAGTTCATTCTTTCAAGAAAGACCAATAGTAGGCCAGCTCATATTATTTTTTATTCATATTTTGAAGTCTATAATCCTAAACTGGTTTGATTTGCAACTTCAACTCAACTTTGAGTTTATTTTTTATTGGATCTCATAGGCCAGCTCTTAACTTTTTATTTAATATCTTAATGAATCAGTTAATCTATACTTTCAATTCATGAAAAATATTTAACTTTGTGGTTCATCTTTTGGTCTTACCTAATCTAATCATTGTTTATCAATTTCAGAAACCATCAAGGAAAGAACCTACCTCAGTACTTGATTATGAAGAAGCCTCAAAACCCACCACAGAGGATTTCAACACTCCCACCACATCAGAAACAGAAACTTCTCAACTGAATTCCACGAAAGTTGATGAACAAAGTGATATTTCCCAGGACAAAGCTCCAAACAAAATTCTTCCATGTCCAAGATGCAAAAGCATAGACACAAAATTTTGTTACTTCAATAATTACAATGCCAACCAGCCCCGTCACTTCTGCAAAAACTGTCAAAGATATTGGACTGCCGGAGGAACCACGAGAAGTATGCTTGTAGGCGCAGGTCGTCGCAAGAACAAAATCTCTTCTTTTTCTTCCGATGCCTCACATAATCGTCAAATGAGTACTGTCTTGAACTTTGGATCAGATTCTCCTATTATGTCTTCAACTTCACTCGACAAGAAAATGAATGTTGGATCAGAAGGTGAAACATCTGGTAAGAGTAATCAGTGTTTTTTTCCACAACAGTTTCCATGGAACCCAGCAATGTGTTATCCTGTTTCATTCCAACCCAATATAGCATACTATGGGGGTTGTTTGGTCCCATCATGGAGCGTACAACCTATCGCAACTCAATCTTGTGTTCCAAGTAAACCTACTTTAGGGAAACATTCAAGAGATGGCTTAGAAAATAACAAGAAAGAAAGTGATAATAATAGTGTGTTGATTCCTAAGAAATTAAGAATTGAAGACCCTAATGAAGCTGCAAAGGGTTCTAATTGGTTAACACTTGGAATCAAAAGTGGTGGAGCATTTTTCAATGGATTTGCATCTATGGGTGGTGATAAGAATCATGTGGTTGAAGCAGCCTCATCAGTATTGAAAGCCAATCCTGCAGCTTTATCAAGGTCTTTTGTGTTCCATGAGAGGATTTGA
Protein sequence

>MS.gene76605.t1

MTNDSTIKLFGRTIFQTHNTDDCSSEFSPPLAHEDFSDHSLHSSLSSSSPLEVNSSKRYKKPSRKEPTSVLDYEEASKPTTEDFNTPTTSETETSQLNSTKVDEQSDISQDKAPNKILPCPRCKSIDTKFCYFNNYNANQPRHFCKNCQRYWTAGGTTRSMLVGAGRRKNKISSFSSDASHNRQMSTVLNFGSDSPIMSSTSLDKKMNVGSEGETSGKSNQCFFPQQFPWNPAMCYPVSFQPNIAYYGGCLVPSWSVQPIATQSCVPSKPTLGKHSRDGLENNKKESDNNSVLIPKKLRIEDPNEAAKGSNWLTLGIKSGGAFFNGFASMGGDKNHVVEAASSVLKANPAALSRSFVFHERI