AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76606

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76606.t1 MTR_5g041420 92.672 232 17 0 132 363 57 288 1.87e-152 432
MS.gene76606.t1 MTR_5g041530 74.123 228 46 3 132 354 56 275 3.64e-109 324
MS.gene76606.t1 MTR_5g041380 70.588 238 50 5 130 362 54 276 4.41e-108 321
MS.gene76606.t1 MTR_5g041400 68.261 230 53 5 138 362 54 268 2.10e-103 309
MS.gene76606.t1 MTR_4g082060 38.095 315 99 14 132 359 37 342 5.78e-47 166
MS.gene76606.t1 MTR_7g010950 44.444 189 68 5 154 319 72 246 1.09e-39 147
MS.gene76606.t1 MTR_6g012450 45.455 165 68 4 154 296 75 239 6.01e-37 139
MS.gene76606.t1 MTR_3g435480 49.286 140 48 3 176 292 97 236 1.83e-33 129
MS.gene76606.t1 MTR_7g086780 52.800 125 34 3 195 296 81 203 1.90e-33 129
MS.gene76606.t1 MTR_6g027460 55.670 97 40 1 173 266 53 149 6.50e-32 123
MS.gene76606.t1 MTR_8g044220 67.568 74 24 0 193 266 98 171 7.60e-31 122
MS.gene76606.t1 MTR_6g027450 53.125 96 42 1 174 266 47 142 5.97e-29 114
MS.gene76606.t1 MTR_2g016030 70.000 60 18 0 207 266 41 100 2.36e-28 108
MS.gene76606.t1 MTR_2g093220 63.636 66 23 1 202 266 28 93 4.76e-24 100
MS.gene76606.t1 MTR_3g091820 57.143 70 30 0 200 269 30 99 8.85e-24 100
MS.gene76606.t1 MTR_8g079060 49.451 91 35 1 205 284 21 111 2.21e-23 97.4
MS.gene76606.t1 MTR_8g015840 62.500 64 23 1 202 264 8 71 9.17e-23 95.5
MS.gene76606.t1 MTR_2g096740 55.714 70 31 0 197 266 46 115 1.17e-22 96.7
MS.gene76606.t1 MTR_2g096740 55.714 70 31 0 197 266 61 130 1.37e-22 96.7
MS.gene76606.t1 MTR_4g022370 48.684 76 39 0 191 266 60 135 2.03e-22 97.4
MS.gene76606.t1 MTR_8g027295 61.017 59 23 0 208 266 19 77 2.17e-22 95.5
MS.gene76606.t1 MTR_4g461080 61.538 65 24 1 202 265 17 81 3.18e-22 95.1
MS.gene76606.t1 MTR_8g479350 57.333 75 31 1 211 284 66 140 5.35e-22 95.9
MS.gene76606.t1 MTR_4g109980 67.925 53 17 0 211 263 38 90 1.29e-21 94.4
MS.gene76606.t1 MTR_3g090430 57.746 71 28 2 198 266 34 104 1.32e-21 94.7
MS.gene76606.t1 MTR_1g077600 59.375 64 25 1 203 265 32 95 1.44e-21 93.2
MS.gene76606.t1 MTR_1g077600 59.375 64 25 1 203 265 32 95 1.57e-21 93.6
MS.gene76606.t1 MTR_4g089095 67.925 53 17 0 215 267 52 104 1.79e-21 93.6
MS.gene76606.t1 MTR_4g089095 67.925 53 17 0 215 267 18 70 2.43e-21 92.4
MS.gene76606.t1 MTR_7g024670 70.588 51 15 0 215 265 79 129 2.70e-21 94.4
MS.gene76606.t1 MTR_8g068210 55.714 70 31 0 195 264 14 83 2.87e-21 93.6
MS.gene76606.t1 MTR_1g056810 63.333 60 21 1 208 266 6 65 4.54e-21 92.4
MS.gene76606.t1 MTR_4g063780 61.404 57 22 0 209 265 71 127 6.89e-21 92.4
MS.gene76606.t1 MTR_2g013370 61.404 57 22 0 211 267 44 100 7.01e-21 91.7
MS.gene76606.t1 MTR_4g088580 69.231 52 16 0 215 266 57 108 7.66e-21 93.2
MS.gene76606.t1 MTR_2g059540 68.000 50 16 0 212 261 30 79 7.70e-21 89.7
MS.gene76606.t1 MTR_5g031440 65.455 55 19 0 209 263 39 93 1.54e-20 91.7
MS.gene76606.t1 MTR_7g059400 63.462 52 19 0 215 266 78 129 1.61e-19 89.0
MS.gene76606.t1 MTR_2g014170 66.000 50 17 0 212 261 44 93 2.35e-19 87.8
MS.gene76606.t1 MTR_3g077750 38.136 118 55 2 146 263 12 111 2.74e-19 88.2
MS.gene76606.t1 MTR_2g014060 70.000 50 15 0 215 264 27 76 3.26e-19 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76606.t1 AT1G69570 44.048 168 62 6 207 348 126 287 1.03e-37 140
MS.gene76606.t1 AT1G26790 37.443 219 110 7 160 357 59 271 1.28e-35 134
MS.gene76606.t1 AT5G62430 46.203 158 60 7 198 337 38 188 2.88e-34 128
MS.gene76606.t1 AT5G39660 43.931 173 70 5 146 298 74 239 1.08e-33 130
MS.gene76606.t1 AT5G39660 43.931 173 70 5 146 298 74 239 1.08e-33 130
MS.gene76606.t1 AT5G39660 43.931 173 70 5 146 298 74 239 1.08e-33 130
MS.gene76606.t1 AT3G47500 54.054 111 38 1 162 272 72 169 5.58e-32 125
MS.gene76606.t1 AT1G29160 65.217 69 21 1 198 266 50 115 2.50e-30 114
MS.gene76606.t1 AT2G34140 46.018 113 51 3 161 266 2 111 3.99e-30 114
MS.gene76606.t1 AT5G60200 61.538 65 25 0 206 270 48 112 7.63e-24 99.4
MS.gene76606.t1 AT1G51700 60.000 65 25 1 201 264 20 84 2.01e-23 96.7
MS.gene76606.t1 AT2G37590 41.228 114 65 1 193 304 69 182 4.25e-23 99.0
MS.gene76606.t1 AT3G45610 61.538 65 25 0 206 270 33 97 5.10e-23 96.7
MS.gene76606.t1 AT4G24060 59.091 66 27 0 201 266 41 106 5.27e-23 98.6
MS.gene76606.t1 AT3G61850 55.714 70 31 0 201 270 50 119 2.22e-22 95.9
MS.gene76606.t1 AT3G61850 55.714 70 31 0 201 270 50 119 2.22e-22 95.9
MS.gene76606.t1 AT3G61850 55.714 70 31 0 201 270 62 131 2.48e-22 95.9
MS.gene76606.t1 AT3G55370 41.237 97 56 1 174 270 38 133 2.83e-22 96.3
MS.gene76606.t1 AT3G55370 41.237 97 56 1 174 270 83 178 3.29e-22 97.1
MS.gene76606.t1 AT3G61850 55.714 70 31 0 201 270 50 119 3.58e-22 95.9
MS.gene76606.t1 AT3G61850 55.714 70 31 0 201 270 62 131 3.64e-22 96.3
MS.gene76606.t1 AT3G55370 41.237 97 56 1 174 270 38 133 4.05e-22 96.3
MS.gene76606.t1 AT2G28810 43.182 88 48 1 177 264 43 128 5.93e-22 95.5
MS.gene76606.t1 AT3G50410 69.231 52 16 0 213 264 30 81 7.66e-22 93.6
MS.gene76606.t1 AT2G28810 43.182 88 48 1 177 264 60 145 8.13e-22 95.5
MS.gene76606.t1 AT5G60850 63.333 60 22 0 211 270 49 108 9.94e-22 94.7
MS.gene76606.t1 AT3G21270 70.588 51 15 0 215 265 31 81 1.72e-21 91.7
MS.gene76606.t1 AT1G07640 68.627 51 16 0 215 265 23 73 3.09e-21 92.4
MS.gene76606.t1 AT1G07640 68.627 51 16 0 215 265 79 129 3.75e-21 93.2
MS.gene76606.t1 AT1G07640 68.627 51 16 0 215 265 87 137 4.40e-21 93.2
MS.gene76606.t1 AT5G66940 60.000 60 24 0 206 265 25 84 4.42e-21 90.9
MS.gene76606.t1 AT2G28510 66.071 56 19 0 215 270 50 105 5.97e-21 92.0
MS.gene76606.t1 AT1G64620 56.250 64 27 1 198 261 35 97 1.63e-20 91.7
MS.gene76606.t1 AT1G28310 64.815 54 19 0 213 266 27 80 2.76e-20 90.5
MS.gene76606.t1 AT3G52440 61.404 57 21 1 211 266 22 78 3.07e-20 89.4
MS.gene76606.t1 AT1G28310 64.815 54 19 0 213 266 51 104 3.61e-20 90.5
MS.gene76606.t1 AT1G47655 67.347 49 16 0 213 261 29 77 3.69e-20 88.2
MS.gene76606.t1 AT5G65590 63.793 58 21 0 211 268 39 96 3.84e-20 90.1
MS.gene76606.t1 AT3G52440 61.404 57 21 1 211 266 41 97 4.01e-20 89.4
MS.gene76606.t1 AT4G38000 64.286 56 19 1 212 266 40 95 7.22e-20 88.2
MS.gene76606.t1 AT5G62940 40.984 122 48 2 215 312 75 196 9.34e-20 89.7
MS.gene76606.t1 AT5G02460 58.621 58 24 0 209 266 91 148 1.05e-19 90.1
MS.gene76606.t1 AT4G00940 57.627 59 25 0 208 266 63 121 1.09e-19 88.6
MS.gene76606.t1 AT4G00940 57.627 59 25 0 208 266 63 121 1.09e-19 88.6
MS.gene76606.t1 AT4G00940 57.627 59 25 0 208 266 63 121 1.09e-19 88.6
MS.gene76606.t1 AT1G21340 66.000 50 17 0 215 264 40 89 1.56e-19 87.4
MS.gene76606.t1 AT2G46590 60.377 53 21 0 208 260 63 115 4.38e-19 87.8
MS.gene76606.t1 AT2G46590 60.377 53 21 0 208 260 75 127 4.94e-19 87.8
MS.gene76606.t1 AT4G21050 54.688 64 29 0 201 264 12 75 2.49e-18 83.2
MS.gene76606.t1 AT4G21040 63.462 52 19 0 215 266 27 78 4.06e-17 80.1
MS.gene76606.t1 AT4G21080 63.462 52 19 0 215 266 27 78 1.76e-16 78.6

Find 72 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCGCAAGCATTCACAAATT+TGG 0.186595 5.1:-31230568 None:intergenic
TTTCCTTAAAGTTAGTTATT+TGG 0.224729 5.1:-31233204 None:intergenic
CCACCATAGAATGCTATATT+TGG 0.230148 5.1:-31233129 None:intergenic
ACAACTTGGTCTGGTTTCTT+TGG 0.248772 5.1:-31232736 None:intergenic
CTGTTGAATCATGGTACTTC+TGG 0.253813 5.1:-31230440 None:intergenic
TAATACTCTTCCAAGAATTC+AGG 0.253989 5.1:+31230389 MS.gene76606:CDS
TTTGGAAACCCCTGAATTCT+TGG 0.273548 5.1:-31230550 None:intergenic
AAATACTCTTCCAAGAATTC+AGG 0.276905 5.1:+31230540 MS.gene76606:CDS
TGTGGAAACCCCTGAATTCT+TGG 0.279554 5.1:-31230399 None:intergenic
CTCCAGAAGTCCAGTATCTT+TGG 0.293736 5.1:-31232849 None:intergenic
TGCCAAAGATACTGGACTTC+TGG 0.300675 5.1:+31232847 MS.gene76606:CDS
AATGCTATATTTGGATAGAT+TGG 0.337846 5.1:-31233120 None:intergenic
ATGAGTACTGTCTTGACATT+TGG 0.342898 5.1:+31232961 MS.gene76606:CDS
TATCCAAATATAGCATTCTA+TGG 0.352424 5.1:+31233126 MS.gene76606:CDS
AGTGAAGTTGAAGACATAAT+AGG 0.361504 5.1:-31232994 None:intergenic
TTCTGCTGCAAGAAAATCTA+AGG 0.372714 5.1:+31230865 MS.gene76606:CDS
TTCTTGCAGCAGAATGCGGC+TGG 0.374115 5.1:-31230856 None:intergenic
ACATGATGCAAAGAGATATA+AGG 0.388068 5.1:+31231883 MS.gene76606:CDS
ATTCTGAATCCTCTGTGGTT+TGG 0.395071 5.1:-31232597 None:intergenic
AGCATTCTATGGTGGTTGTT+TGG 0.399605 5.1:+31233137 MS.gene76606:CDS
GGACATGGAACAACTTGGTC+TGG 0.406395 5.1:-31232745 None:intergenic
GTGGTTGTTTGGTACCATCT+TGG 0.412555 5.1:+31233148 MS.gene76606:CDS
CAAGAAAATCTAAGGACAAT+GGG 0.417276 5.1:+31230873 MS.gene76606:CDS
AGGTTTGAATGCCTCTCTAC+TGG 0.428831 5.1:-31230908 None:intergenic
ACCACGAGAAATATGCTTAT+AGG 0.437951 5.1:+31232874 MS.gene76606:CDS
ACTGTGTTGAGATGGATTGT+AGG 0.456181 5.1:-31233173 None:intergenic
GTTGAAGAATTCACTTCAAG+TGG 0.481761 5.1:-31231857 None:intergenic
AATACTCTTCCAAGAATTCA+GGG 0.489964 5.1:+31230390 MS.gene76606:CDS
AATACTCTTCCAAGAATTCA+GGG 0.494093 5.1:+31230541 MS.gene76606:CDS
TAGAGAGGCATTCAAACCTT+AGG 0.498363 5.1:+31230912 MS.gene76606:CDS
TACTGTCTTGACATTTGGAT+CGG 0.502009 5.1:+31232966 MS.gene76606:CDS
GGCTCTTGGCTGTTGAATCA+TGG 0.507282 5.1:-31230449 None:intergenic
TTTACGTATAAAAGTTCTTG+TGG 0.507739 5.1:-31230417 None:intergenic
AAAAGAACTCATCGAGGATG+AGG 0.512018 5.1:+31232567 MS.gene76606:CDS
GACGTCTCTGTTTCAGATGA+GGG 0.514212 5.1:-31232658 None:intergenic
GTGTGAGGACAGTAGGCTCT+TGG 0.528266 5.1:-31230463 None:intergenic
TTCATTAGACTGTGTTGAGA+TGG 0.528897 5.1:-31233181 None:intergenic
AGAAATATGCTTATAGGCGC+AGG 0.536173 5.1:+31232880 MS.gene76606:CDS
ATATTTGGATAGATTGGCAC+AGG 0.538462 5.1:-31233114 None:intergenic
TAACACATAGCAGGATTCCA+TGG 0.544411 5.1:-31233090 None:intergenic
GCAAGAAAATCTAAGGACAA+TGG 0.545927 5.1:+31230872 MS.gene76606:CDS
CACACTGCACGTGTCTTCCA+AGG 0.553355 5.1:+31230481 MS.gene76606:CDS
ACGTGCAGTGTGAGGACAGT+AGG 0.558802 5.1:-31230470 None:intergenic
CGCAAGTCAACAACTTCAAG+AGG 0.564603 5.1:+31230590 MS.gene76606:CDS
AGACGTCTCTGTTTCAGATG+AGG 0.571871 5.1:-31232659 None:intergenic
AAGAAGAGATTCTGTTCTTG+CGG 0.572733 5.1:-31232906 None:intergenic
ATCTTGGACATGGAACAACT+TGG 0.574876 5.1:-31232750 None:intergenic
TCAATAAGGCTTGAAGATGT+CGG 0.575632 5.1:-31232622 None:intergenic
GCCTATAAGCATATTTCTCG+TGG 0.581874 5.1:-31232875 None:intergenic
TCTTGCAGCAGAATGCGGCT+GGG 0.584089 5.1:-31230855 None:intergenic
GGCACAGGATAACACATAGC+AGG 0.586834 5.1:-31233099 None:intergenic
AAGAGTATTTACCTTGACAA+AGG 0.590247 5.1:-31230527 None:intergenic
GGATTCCATGGAAACTGTTG+AGG 0.594593 5.1:-31233078 None:intergenic
CCACTACATCAGCAAACGAA+GGG 0.601896 5.1:+31230827 MS.gene76606:CDS
ATGGATTGTAGGCTCCAAGA+TGG 0.604901 5.1:-31233162 None:intergenic
CCCTTCGTTTGCTGATGTAG+TGG 0.607194 5.1:-31230827 None:intergenic
AATTCAAGGATCCTTTGTCA+AGG 0.617783 5.1:+31230516 MS.gene76606:CDS
TGGAAGACACGTGCAGTGTG+AGG 0.628448 5.1:-31230478 None:intergenic
CGGAGATTCTGAATCCTCTG+TGG 0.629252 5.1:-31232602 None:intergenic
ATACTCTTCCAAGAATTCAG+GGG 0.630980 5.1:+31230542 MS.gene76606:CDS
ATCGCGATCCTCCAGTAGAG+AGG 0.633435 5.1:+31230897 MS.gene76606:CDS
CAAAGATACTGGACTTCTGG+AGG 0.634659 5.1:+31232850 MS.gene76606:CDS
ATACTCTTCCAAGAATTCAG+GGG 0.640038 5.1:+31230391 MS.gene76606:CDS
CCAAATATAGCATTCTATGG+TGG 0.645659 5.1:+31233129 MS.gene76606:CDS
ACCACTACATCAGCAAACGA+AGG 0.645823 5.1:+31230826 MS.gene76606:CDS
ATGTCCAAGATGCAAAAGCA+CGG 0.662046 5.1:+31232762 MS.gene76606:CDS
TTTGAATGCCTCTCTACTGG+AGG 0.667661 5.1:-31230905 None:intergenic
AAGAAAATCTAAGGACAATG+GGG 0.677742 5.1:+31230874 MS.gene76606:CDS
CACTACATCAGCAAACGAAG+GGG 0.682742 5.1:+31230828 MS.gene76606:CDS
ATCAAGAAAAGAACTCATCG+AGG 0.704142 5.1:+31232561 MS.gene76606:CDS
GGCTTCATTCCAAACCACAG+AGG 0.724092 5.1:+31232588 MS.gene76606:CDS
ACTCATTTGATGATGATGTG+AGG 0.757465 5.1:-31232944 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATAAGAACAAATAAAT+CGG + chr5.1:31232323-31232342 MS.gene76606:intron 10.0%
!! AATACATGTAGTTATATTAT+CGG + chr5.1:31232412-31232431 MS.gene76606:intron 15.0%
!! TAGCATATAATAAAATGAAA+TGG + chr5.1:31231315-31231334 MS.gene76606:intron 15.0%
!!! TATAAGCCAATAAAATTTTA+TGG + chr5.1:31232491-31232510 MS.gene76606:intron 15.0%
!!! TTCAAATTTTTCATCTTTTT+TGG + chr5.1:31231982-31232001 MS.gene76606:intron 15.0%
!!! TTCATTTTTAGGTATTTTAA+TGG + chr5.1:31230682-31230701 MS.gene76606:intron 15.0%
!!! TTTTATAAAGGTAAAAAAGT+TGG - chr5.1:31231536-31231555 None:intergenic 15.0%
!! AATACCTAAAAATGAATATC+AGG - chr5.1:31230678-31230697 None:intergenic 20.0%
!! TGAATAAAATAGACTTCAAA+GGG + chr5.1:31231239-31231258 MS.gene76606:intron 20.0%
!! TTACCTTAATATGAAAACAA+AGG + chr5.1:31231033-31231052 MS.gene76606:intron 20.0%
!! TTGAATAAAATAGACTTCAA+AGG + chr5.1:31231238-31231257 MS.gene76606:intron 20.0%
!!! AAATTTTATGGCTCATATTT+TGG + chr5.1:31232503-31232522 MS.gene76606:intron 20.0%
!!! AGTTTGTTTATCAATTTTAG+TGG - chr5.1:31232695-31232714 None:intergenic 20.0%
!!! TATGAGCCATAAAATTTTAT+TGG - chr5.1:31232500-31232519 None:intergenic 20.0%
!!! TTAAAGTATATCTTTAGTTC+AGG - chr5.1:31231286-31231305 None:intergenic 20.0%
!!! TTACATATTTTACTCAACAT+TGG + chr5.1:31231010-31231029 MS.gene76606:intron 20.0%
! AAACAACAGAAAATTCATGT+TGG - chr5.1:31232301-31232320 None:intergenic 25.0%
! AACAGCTATATATCAATTTC+AGG + chr5.1:31232534-31232553 MS.gene76606:intron 25.0%
! AATAAAATAGACTTCAAAGG+GGG + chr5.1:31231241-31231260 MS.gene76606:intron 25.0%
! AATGCTATATTTGGATAGAT+TGG - chr5.1:31233123-31233142 None:intergenic 25.0%
! ATGTTATCCTCTTAAAAGAA+TGG - chr5.1:31231214-31231233 None:intergenic 25.0%
! GAATAAAAGAATCTAACAAG+AGG + chr5.1:31231337-31231356 MS.gene76606:intron 25.0%
! GAATAAAATAGACTTCAAAG+GGG + chr5.1:31231240-31231259 MS.gene76606:intron 25.0%
! GGACAAAAAAACTAAATTCA+AGG + chr5.1:31230502-31230521 MS.gene76606:CDS 25.0%
! TATCCAAATATAGCATTCTA+TGG + chr5.1:31233126-31233145 MS.gene76606:CDS 25.0%
! TTCTGTTCGAAAAATATACA+CGG + chr5.1:31232085-31232104 MS.gene76606:intron 25.0%
! TTTACGTATAAAAGTTCTTG+TGG - chr5.1:31230420-31230439 None:intergenic 25.0%
!! AATTCAACCATAAAGCTTTT+TGG + chr5.1:31231707-31231726 MS.gene76606:intron 25.0%
!! AGTGAACATTTTATGTATAG+GGG + chr5.1:31230780-31230799 MS.gene76606:intron 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5.1:31231708-31231727 MS.gene76606:intron 25.0%
!! GAGTGAACATTTTATGTATA+GGG + chr5.1:31230779-31230798 MS.gene76606:intron 25.0%
!! TCTGTGAAGATGTTTTATAA+AGG - chr5.1:31231548-31231567 None:intergenic 25.0%
!! TGAGTGAACATTTTATGTAT+AGG + chr5.1:31230778-31230797 MS.gene76606:intron 25.0%
!!! GAATTTAGTTTTTTTGTCCT+TGG - chr5.1:31230501-31230520 None:intergenic 25.0%
!!! TATATTTTGATCCTAACATG+AGG - chr5.1:31232195-31232214 None:intergenic 25.0%
!!! TGGGATCAATATTTTGATAA+GGG + chr5.1:31230803-31230822 MS.gene76606:CDS 25.0%
!!! TGTCCTTTGTTTTCATATTA+AGG - chr5.1:31231039-31231058 None:intergenic 25.0%
!!! TTGATAGTGAAGAGTTATAA+GGG - chr5.1:31231619-31231638 None:intergenic 25.0%
AAAATATGTTGCTAGCTAGT+AGG + chr5.1:31231954-31231973 MS.gene76606:intron 30.0%
AAATACTCTTCCAAGAATTC+AGG + chr5.1:31230540-31230559 MS.gene76606:CDS 30.0%
AAATCTTCATGAGCAATAGT+AGG - chr5.1:31231797-31231816 None:intergenic 30.0%
AAGAAAATCTAAGGACAATG+GGG + chr5.1:31230874-31230893 MS.gene76606:CDS 30.0%
AATACTCTTCCAAGAATTCA+GGG + chr5.1:31230390-31230409 MS.gene76606:CDS 30.0%
AATAGTAGGAGTAAATTCTG+AGG - chr5.1:31231783-31231802 None:intergenic 30.0%
AATTCATGTGACATACATCT+TGG - chr5.1:31230748-31230767 None:intergenic 30.0%
ACATGATGCAAAGAGATATA+AGG + chr5.1:31231883-31231902 MS.gene76606:CDS 30.0%
AGTGAAGTTGAAGACATAAT+AGG - chr5.1:31232997-31233016 None:intergenic 30.0%
CAACTTCACTTCACAAAAAA+GGG + chr5.1:31233007-31233026 MS.gene76606:CDS 30.0%
CAAGAAAATCTAAGGACAAT+GGG + chr5.1:31230873-31230892 MS.gene76606:CDS 30.0%
CCTAAAAATGAATATCAGGA+AGG - chr5.1:31230674-31230693 None:intergenic 30.0%
GCATCTCTCTTTATATTTGT+TGG - chr5.1:31232265-31232284 None:intergenic 30.0%
GGGTGTAATAATAATATGCA+CGG - chr5.1:31230644-31230663 None:intergenic 30.0%
GTTGAATTGTTTATCATCTC+AGG - chr5.1:31231695-31231714 None:intergenic 30.0%
TAACAAGAGGAAATACCAAA+AGG + chr5.1:31231350-31231369 MS.gene76606:intron 30.0%
TAATACTCTTCCAAGAATTC+AGG + chr5.1:31230389-31230408 MS.gene76606:CDS 30.0%
TCAACTTCACTTCACAAAAA+AGG + chr5.1:31233006-31233025 MS.gene76606:CDS 30.0%
TTCTTCAAATAGAATCACCA+TGG - chr5.1:31231920-31231939 None:intergenic 30.0%
! AACATTTTATGTATAGGGGT+GGG + chr5.1:31230784-31230803 MS.gene76606:intron 30.0%
! CTCTCTTTATATTTGTTGGT+AGG - chr5.1:31232261-31232280 None:intergenic 30.0%
! GTTGATGAAATTGTTCATCA+AGG + chr5.1:31232107-31232126 MS.gene76606:intron 30.0%
! TAATGTTTTACCATTCACAC+TGG + chr5.1:31231111-31231130 MS.gene76606:intron 30.0%
! TACATGTATTGACATCATGT+TGG - chr5.1:31232402-31232421 None:intergenic 30.0%
! TTTCACTCCATTCTTTTAAG+AGG + chr5.1:31231204-31231223 MS.gene76606:intron 30.0%
!! AAGAGAGATGCTTTTTATCA+AGG + chr5.1:31232274-31232293 MS.gene76606:intron 30.0%
!! AAGAGTATTTACCTTGACAA+AGG - chr5.1:31230530-31230549 None:intergenic 30.0%
!! CCTTCCTGATATTCATTTTT+AGG + chr5.1:31230671-31230690 MS.gene76606:intron 30.0%
!! CTTGATAGTGAAGAGTTATA+AGG - chr5.1:31231620-31231639 None:intergenic 30.0%
!!! GTGGGATCAATATTTTGATA+AGG + chr5.1:31230802-31230821 MS.gene76606:CDS 30.0%
AAAGAGAGAAAGAGAGAAAG+AGG - chr5.1:31231589-31231608 None:intergenic 35.0%
AAGAAGAGATTCTGTTCTTG+CGG - chr5.1:31232909-31232928 None:intergenic 35.0%
AAGAGGAAATACCAAAAGGA+AGG + chr5.1:31231354-31231373 MS.gene76606:intron 35.0%
AATTCAAGGATCCTTTGTCA+AGG + chr5.1:31230516-31230535 MS.gene76606:CDS 35.0%
ACCACGAGAAATATGCTTAT+AGG + chr5.1:31232874-31232893 MS.gene76606:CDS 35.0%
ATACTCTTCCAAGAATTCAG+GGG + chr5.1:31230391-31230410 MS.gene76606:CDS 35.0%
ATAGTAACAAGAGACCTTGA+TGG - chr5.1:31232232-31232251 None:intergenic 35.0%
ATATTTGGATAGATTGGCAC+AGG - chr5.1:31233117-31233136 None:intergenic 35.0%
ATCAAGAAAAGAACTCATCG+AGG + chr5.1:31232561-31232580 MS.gene76606:CDS 35.0%
CAAATAGAATCACCATGGTA+TGG - chr5.1:31231915-31231934 None:intergenic 35.0%
CCAAATATAGCATTCTATGG+TGG + chr5.1:31233129-31233148 MS.gene76606:CDS 35.0%
CCACCATAGAATGCTATATT+TGG - chr5.1:31233132-31233151 None:intergenic 35.0%
CTTCACAAAAAAGGGAATGT+TGG + chr5.1:31233015-31233034 MS.gene76606:CDS 35.0%
GCAAAAATTGCCAAAGATAC+TGG + chr5.1:31232839-31232858 MS.gene76606:CDS 35.0%
GCAAGAAAATCTAAGGACAA+TGG + chr5.1:31230872-31230891 MS.gene76606:CDS 35.0%
GTTGAAGAATTCACTTCAAG+TGG - chr5.1:31231860-31231879 None:intergenic 35.0%
TAATAATATGCACGGTTGCA+TGG - chr5.1:31230636-31230655 None:intergenic 35.0%
TACCAAAAGGAAGGAACAAA+AGG + chr5.1:31231363-31231382 MS.gene76606:intron 35.0%
TACTGTCTTGACATTTGGAT+CGG + chr5.1:31232966-31232985 MS.gene76606:CDS 35.0%
TATATGAGCAACCTCATGTT+AGG + chr5.1:31232181-31232200 MS.gene76606:intron 35.0%
TCAATAAGGCTTGAAGATGT+CGG - chr5.1:31232625-31232644 None:intergenic 35.0%
TGCAATAAGTGGAAGATACA+TGG - chr5.1:31231173-31231192 None:intergenic 35.0%
TTCATTAGACTGTGTTGAGA+TGG - chr5.1:31233184-31233203 None:intergenic 35.0%
TTCTGCTGCAAGAAAATCTA+AGG + chr5.1:31230865-31230884 MS.gene76606:CDS 35.0%
! ATGAGTACTGTCTTGACATT+TGG + chr5.1:31232961-31232980 MS.gene76606:CDS 35.0%
! GAACATTTTATGTATAGGGG+TGG + chr5.1:31230783-31230802 MS.gene76606:intron 35.0%
! TCAAGAGGTATGTTACCTTT+AGG + chr5.1:31230605-31230624 MS.gene76606:intron 35.0%
! TTGTGAGCCACAAATTTTTG+TGG - chr5.1:31231482-31231501 None:intergenic 35.0%
!! AAACTTCCACTTTTCTGATG+TGG + chr5.1:31231501-31231520 MS.gene76606:intron 35.0%
!! ACTCATTTGATGATGATGTG+AGG - chr5.1:31232947-31232966 None:intergenic 35.0%
!!! GAGTTTTCAGGTTCTCAATA+AGG - chr5.1:31232639-31232658 None:intergenic 35.0%
AAAAGAACTCATCGAGGATG+AGG + chr5.1:31232567-31232586 MS.gene76606:CDS 40.0%
AACACATCTCTTTCCATGCA+AGG + chr5.1:31230709-31230728 MS.gene76606:intron 40.0%
AATATCAGGAAGGACTTGCA+GGG - chr5.1:31230664-31230683 None:intergenic 40.0%
AGAAATATGCTTATAGGCGC+AGG + chr5.1:31232880-31232899 MS.gene76606:CDS 40.0%
AGCATTCTATGGTGGTTGTT+TGG + chr5.1:31233137-31233156 MS.gene76606:CDS 40.0%
ATAAGGTATGTGCCATACCA+TGG + chr5.1:31231900-31231919 MS.gene76606:intron 40.0%
ATCTTGGACATGGAACAACT+TGG - chr5.1:31232753-31232772 None:intergenic 40.0%
ATGTCCAAGATGCAAAAGCA+CGG + chr5.1:31232762-31232781 MS.gene76606:CDS 40.0%
ATTCTGAATCCTCTGTGGTT+TGG - chr5.1:31232600-31232619 None:intergenic 40.0%
CAAAAGGAAGGAACAAAAGG+AGG + chr5.1:31231366-31231385 MS.gene76606:intron 40.0%
CGTTCTCCCAAAAAGCTTTA+TGG - chr5.1:31231717-31231736 None:intergenic 40.0%
CTCGATGTTTGTGTATGTGT+CGG + chr5.1:31232442-31232461 MS.gene76606:intron 40.0%
CTGTTGAATCATGGTACTTC+TGG - chr5.1:31230443-31230462 None:intergenic 40.0%
GCAATGTGATGTGCAATAAG+TGG - chr5.1:31231184-31231203 None:intergenic 40.0%
GCCTATAAGCATATTTCTCG+TGG - chr5.1:31232878-31232897 None:intergenic 40.0%
GTCGCAAGCATTCACAAATT+TGG - chr5.1:31230571-31230590 None:intergenic 40.0%
GTGACATGGATGAAAAAGGT+AGG + chr5.1:31231388-31231407 MS.gene76606:intron 40.0%
TAACACATAGCAGGATTCCA+TGG - chr5.1:31233093-31233112 None:intergenic 40.0%
TCACAGGCCACAAAAATTTG+TGG + chr5.1:31231472-31231491 MS.gene76606:intron 40.0%
TGAAAGAGATCTGTCCATCA+AGG + chr5.1:31232215-31232234 MS.gene76606:intron 40.0%
TGAGTTTCACTGCGATTTCT+CGG + chr5.1:31232046-31232065 MS.gene76606:intron 40.0%
TTTGGAAACCCCTGAATTCT+TGG - chr5.1:31230553-31230572 None:intergenic 40.0%
! ACAACTTGGTCTGGTTTCTT+TGG - chr5.1:31232739-31232758 None:intergenic 40.0%
! CTCCTTTTGTTCCTTCCTTT+TGG - chr5.1:31231368-31231387 None:intergenic 40.0%
! GATTTTCTTGCAGCAGAATG+CGG - chr5.1:31230863-31230882 None:intergenic 40.0%
! GTTTTCCTCAACAGTTTCCA+TGG + chr5.1:31233073-31233092 MS.gene76606:CDS 40.0%
! TTTCAGATGAGGGAGTTTTC+AGG - chr5.1:31232651-31232670 None:intergenic 40.0%
!! ACTGTGTTGAGATGGATTGT+AGG - chr5.1:31233176-31233195 None:intergenic 40.0%
!! TAGAGAGGCATTCAAACCTT+AGG + chr5.1:31230912-31230931 MS.gene76606:CDS 40.0%
!!! CAATTTTTGCAGAAGTGTCG+AGG - chr5.1:31232829-31232848 None:intergenic 40.0%
ACCACTACATCAGCAAACGA+AGG + chr5.1:31230826-31230845 MS.gene76606:CDS 45.0%
AGACGTCTCTGTTTCAGATG+AGG - chr5.1:31232662-31232681 None:intergenic 45.0%
AGGAACAAAAGGAGGTGACA+TGG + chr5.1:31231374-31231393 MS.gene76606:intron 45.0%
AGGTTTGAATGCCTCTCTAC+TGG - chr5.1:31230911-31230930 None:intergenic 45.0%
AGTTTCACTGCGATTTCTCG+GGG + chr5.1:31232048-31232067 MS.gene76606:intron 45.0%
CAAAGATACTGGACTTCTGG+AGG + chr5.1:31232850-31232869 MS.gene76606:CDS 45.0%
CACTACATCAGCAAACGAAG+GGG + chr5.1:31230828-31230847 MS.gene76606:CDS 45.0%
CCACTACATCAGCAAACGAA+GGG + chr5.1:31230827-31230846 MS.gene76606:CDS 45.0%
CGCAAGTCAACAACTTCAAG+AGG + chr5.1:31230590-31230609 MS.gene76606:CDS 45.0%
CTAACTCTCAACTCCTTGCA+TGG - chr5.1:31230725-31230744 None:intergenic 45.0%
CTCCAGAAGTCCAGTATCTT+TGG - chr5.1:31232852-31232871 None:intergenic 45.0%
GAATATCAGGAAGGACTTGC+AGG - chr5.1:31230665-31230684 None:intergenic 45.0%
GACGTCTCTGTTTCAGATGA+GGG - chr5.1:31232661-31232680 None:intergenic 45.0%
GAGTTTCACTGCGATTTCTC+GGG + chr5.1:31232047-31232066 MS.gene76606:intron 45.0%
GCAATGCCACATCAGAAAAG+TGG - chr5.1:31231510-31231529 None:intergenic 45.0%
GGAGGTGACATGGATGAAAA+AGG + chr5.1:31231384-31231403 MS.gene76606:intron 45.0%
GGATTCCATGGAAACTGTTG+AGG - chr5.1:31233081-31233100 None:intergenic 45.0%
GGTTGCATGGTCATTCCTAA+AGG - chr5.1:31230623-31230642 None:intergenic 45.0%
GTGAGATCTTAGTGTGCAGA+TGG - chr5.1:31231456-31231475 None:intergenic 45.0%
GTGGTTGTTTGGTACCATCT+TGG + chr5.1:31233148-31233167 MS.gene76606:CDS 45.0%
TCTGCACACTAAGATCTCAC+AGG + chr5.1:31231456-31231475 MS.gene76606:intron 45.0%
TGCCAAAGATACTGGACTTC+TGG + chr5.1:31232847-31232866 MS.gene76606:CDS 45.0%
TGTGGAAACCCCTGAATTCT+TGG - chr5.1:31230402-31230421 None:intergenic 45.0%
TTTGAATGCCTCTCTACTGG+AGG - chr5.1:31230908-31230927 None:intergenic 45.0%
! ATGGATTGTAGGCTCCAAGA+TGG - chr5.1:31233165-31233184 None:intergenic 45.0%
! GTGCTTTTGCATCTTGGACA+TGG - chr5.1:31232763-31232782 None:intergenic 45.0%
!! TTTATTATAACATATTATTA+AGG - chr5.1:31230987-31231006 None:intergenic 5.0%
CGATCTCTAGCCAGTGTGAA+TGG - chr5.1:31231124-31231143 None:intergenic 50.0%
CGGAGATTCTGAATCCTCTG+TGG - chr5.1:31232605-31232624 None:intergenic 50.0%
GGACATGGAACAACTTGGTC+TGG - chr5.1:31232748-31232767 None:intergenic 50.0%
GGCACAGGATAACACATAGC+AGG - chr5.1:31233102-31233121 None:intergenic 50.0%
GGCTCTTGGCTGTTGAATCA+TGG - chr5.1:31230452-31230471 None:intergenic 50.0%
GGCTTCATTCCAAACCACAG+AGG + chr5.1:31232588-31232607 MS.gene76606:CDS 50.0%
TTACACATGCGCTGCACCTA+AGG - chr5.1:31230931-31230950 None:intergenic 50.0%
! CCCTTCGTTTGCTGATGTAG+TGG - chr5.1:31230830-31230849 None:intergenic 50.0%
! GTGTCCGTGCTTTTGCATCT+TGG - chr5.1:31232769-31232788 None:intergenic 50.0%
ACGTGCAGTGTGAGGACAGT+AGG - chr5.1:31230473-31230492 None:intergenic 55.0%
ATCGCGATCCTCCAGTAGAG+AGG + chr5.1:31230897-31230916 MS.gene76606:CDS 55.0%
CACACTGCACGTGTCTTCCA+AGG + chr5.1:31230481-31230500 MS.gene76606:CDS 55.0%
GTGTGAGGACAGTAGGCTCT+TGG - chr5.1:31230466-31230485 None:intergenic 55.0%
TCTTGCAGCAGAATGCGGCT+GGG - chr5.1:31230858-31230877 None:intergenic 55.0%
TGGAAGACACGTGCAGTGTG+AGG - chr5.1:31230481-31230500 None:intergenic 55.0%
TTCTTGCAGCAGAATGCGGC+TGG - chr5.1:31230859-31230878 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 31230384 31233215 31230384 ID=MS.gene76606
chr5.1 mRNA 31230384 31233215 31230384 ID=MS.gene76606.t1;Parent=MS.gene76606
chr5.1 exon 31230384 31230611 31230384 ID=MS.gene76606.t1.exon1;Parent=MS.gene76606.t1
chr5.1 CDS 31230384 31230611 31230384 ID=cds.MS.gene76606.t1;Parent=MS.gene76606.t1
chr5.1 exon 31230800 31230933 31230800 ID=MS.gene76606.t1.exon2;Parent=MS.gene76606.t1
chr5.1 CDS 31230800 31230933 31230800 ID=cds.MS.gene76606.t1;Parent=MS.gene76606.t1
chr5.1 exon 31231832 31231904 31231832 ID=MS.gene76606.t1.exon3;Parent=MS.gene76606.t1
chr5.1 CDS 31231832 31231904 31231832 ID=cds.MS.gene76606.t1;Parent=MS.gene76606.t1
chr5.1 exon 31232556 31233215 31232556 ID=MS.gene76606.t1.exon4;Parent=MS.gene76606.t1
chr5.1 CDS 31232556 31233215 31232556 ID=cds.MS.gene76606.t1;Parent=MS.gene76606.t1
Gene Sequence

>MS.gene76606

ATGGTTAATACTCTTCCAAGAATTCAGGGGTTTCCACAAGAACTTTTATACGTAAACCAGAAGTACCATGATTCAACAGCCAAGAGCCTACTGTCCTCACACTGCACGTGTCTTCCAAGGACAAAAAAACTAAATTCAAGGATCCTTTGTCAAGGTAAATACTCTTCCAAGAATTCAGGGGTTTCCAAATTTGTGAATGCTTGCGACGCAAGTCAACAACTTCAAGAGGTATGTTACCTTTAGGAATGACCATGCAACCGTGCATATTATTATTACACCCTGCAAGTCCTTCCTGATATTCATTTTTAGGTATTTTAATGGCAACAACACATCTCTTTCCATGCAAGGAGTTGAGAGTTAGCCAAGATGTATGTCACATGAATTGTTTAGAACTTGAGTGAACATTTTATGTATAGGGGTGGGATCAATATTTTGATAAGGGACCACTACATCAGCAAACGAAGGGGACAACCCAGCCGCATTCTGCTGCAAGAAAATCTAAGGACAATGGGGATCGCGATCCTCCAGTAGAGAGGCATTCAAACCTTAGGTGCAGCGCATGTGTAACAAAAGAAGTTCATTTTAATAAATTCGCTTATACCTTAATAATATGTTATAATAAAGTTTTACATATTTTACTCAACATTGGTTACCTTAATATGAAAACAAAGGACAAGTGACTTGTGCGTATACATAGTTATTTTGTAAGTTTTATTTATTTATAAACTAATGTTTTACCATTCACACTGGCTAGAGATCGAACTTTCGATCAAATAGCTAACTGATCCATGTATCTTCCACTTATTGCACATCACATTGCTTTCACTCCATTCTTTTAAGAGGATAACATTTCTTTGAATAAAATAGACTTCAAAGGGGGAAGAAAAATAAGAGTAAATCCTGAACTAAAGATATACTTTAATTGTTGATGTAGCATATAATAAAATGAAATGGAATAAAAGAATCTAACAAGAGGAAATACCAAAAGGAAGGAACAAAAGGAGGTGACATGGATGAAAAAGGTAGGACATAAGAGAACAACACTATCTTTATAATTATGTGTGTGAATCCATCTGCACACTAAGATCTCACAGGCCACAAAAATTTGTGGCTCACAAAACTTCCACTTTTCTGATGTGGCATTGCTTTCCAACTTTTTTACCTTTATAAAACATCTTCACAGAAACACATAAACTATTGTACCTCTTTCTCTCTTTCTCTCTTTTTCTTCTCCCTTATAACTCTTCACTATCAAGCAAACAGAACAAAGAAATAACAGCAACAACAAAATCTTCTTCTACACATATACCTGAGATGATAAACAATTCAACCATAAAGCTTTTTGGGAGAACGATTTTTCAAACACACAACACTGATGTGTCTATCAATGATTCTTCCTCAGAATTTACTCCTACTATTGCTCATGAAGATTTCTCTGATCATAGTAGTCATTCATCTTTATCTGCATCTTCTCCACTTGAAGTGAATTCTTCAACTGAACATGATGCAAAGAGATATAAGGTATGTGCCATACCATGGTGATTCTATTTGAAGAATTATATTTTCTTGAAAAATATGTTGCTAGCTAGTAGGTTTAATTCAAATTTTTCATCTTTTTTGGTTTGAAATAAATTGCTTCTAGTTACGTGTATGCAATTAAATTGAGTTTCACTGCGATTTCTCGGGGCTGTGTTGACAACTTTCTGTTCGAAAAATATACACGGTTGATGAAATTGTTCATCAAGGTCTCATTCAACTTATGCACAAAAAACTTGTCTCTTTATGTGAACAATTTCATATATGAGCAACCTCATGTTAGGATCAAAATATATGAAAGAGATCTGTCCATCAAGGTCTCTTGTTACTATTTCGATCCTACCAACAAATATAAAGAGAGATGCTTTTTATCAAGGACCAACATGAATTTTCTGTTGTTTATTTATATAAGAACAAATAAATCGGATACTATATGCTTATGCAGCACTGACACTTGAGAATAAAGTTGTGTTTTGTGTCCAACATGATGTCAATACATGTAGTTATATTATCGGTGTCTTACTCGATGTTTGTGTATGTGTCGGTGCTTCATAGCTTTATGTTGTCAATTTATAAGCCAATAAAATTTTATGGCTCATATTTTGGTCATATCTAACAGCTATATATCAATTTCAGGAAACATCAAGAAAAGAACTCATCGAGGATGAGGCTTCATTCCAAACCACAGAGGATTCAGAATCTCCGACATCTTCAAGCCTTATTGAGAACCTGAAAACTCCCTCATCTGAAACAGAGACGTCTCAATCAATTTCCACTAAAATTGATAAACAAACTGATATGTCACAAGAAAAATCTCCAAAGAAACCAGACCAAGTTGTTCCATGTCCAAGATGCAAAAGCACGGACACAAAATTTTGCTACTACAACAACTACAACGTCAAACAGCCTCGACACTTCTGCAAAAATTGCCAAAGATACTGGACTTCTGGAGGAACCACGAGAAATATGCTTATAGGCGCAGGTCGCCGCAAGAACAGAATCTCTTCTTCTTTTCATTCTGATACCTCACATCATCATCAAATGAGTACTGTCTTGACATTTGGATCGGATTCTCCTATTATGTCTTCAACTTCACTTCACAAAAAAGGGAATGTTGGTTCACTTGAAAAAACATTTGACAAGAGTTATCAAAGTTTTCCTCAACAGTTTCCATGGAATCCTGCTATGTGTTATCCTGTGCCAATCTATCCAAATATAGCATTCTATGGTGGTTGTTTGGTACCATCTTGGAGCCTACAATCCATCTCAACACAGTCTAATGAACCAAATAACTAA
Protein sequence

>MS.gene76606.t1

MVNTLPRIQGFPQELLYVNQKYHDSTAKSLLSSHCTCLPRTKKLNSRILCQGKYSSKNSGVSKFVNACDASQQLQEGWDQYFDKGPLHQQTKGTTQPHSAARKSKDNGDRDPPVERHSNLSHSSLSASSPLEVNSSTEHDAKRYKETSRKELIEDEASFQTTEDSESPTSSSLIENLKTPSSETETSQSISTKIDKQTDMSQEKSPKKPDQVVPCPRCKSTDTKFCYYNNYNVKQPRHFCKNCQRYWTSGGTTRNMLIGAGRRKNRISSSFHSDTSHHHQMSTVLTFGSDSPIMSSTSLHKKGNVGSLEKTFDKSYQSFPQQFPWNPAMCYPVPIYPNIAFYGGCLVPSWSLQSISTQSNEPNN