Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76816.t1 | XP_003592478.1 | 86.8 | 212 | 10 | 4 | 1 | 212 | 1 | 194 | 3.00E-91 | 344.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76816.t1 | Q9SN23 | 68.4 | 117 | 34 | 1 | 1 | 117 | 1 | 114 | 2.8e-42 | 173.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76816.t1 | G7IE59 | 86.8 | 212 | 10 | 4 | 1 | 212 | 1 | 194 | 2.1e-91 | 344.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene76816.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76816.t1 | MTR_1g106420 | 86.792 | 212 | 10 | 4 | 1 | 212 | 1 | 194 | 4.42e-121 | 341 |
| MS.gene76816.t1 | MTR_4g095600 | 69.421 | 121 | 33 | 2 | 1 | 120 | 1 | 118 | 8.58e-55 | 174 |
| MS.gene76816.t1 | MTR_5g015880 | 64.567 | 127 | 41 | 2 | 1 | 127 | 1 | 123 | 5.52e-54 | 172 |
| MS.gene76816.t1 | MTR_7g007010 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 1.53e-46 | 155 |
| MS.gene76816.t1 | MTR_6g018270 | 43.636 | 165 | 87 | 2 | 1 | 165 | 3 | 161 | 5.44e-45 | 151 |
| MS.gene76816.t1 | MTR_6g453130 | 52.727 | 110 | 42 | 2 | 1 | 104 | 3 | 108 | 1.55e-37 | 130 |
| MS.gene76816.t1 | MTR_1g110840 | 39.157 | 166 | 85 | 5 | 1 | 158 | 3 | 160 | 2.29e-36 | 127 |
| MS.gene76816.t1 | MTR_6g054150 | 50.943 | 106 | 50 | 2 | 1 | 104 | 3 | 108 | 1.60e-34 | 122 |
| MS.gene76816.t1 | MTR_6g054080 | 49.057 | 106 | 52 | 2 | 1 | 104 | 3 | 108 | 7.45e-33 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76816.t1 | AT3G49940 | 68.376 | 117 | 34 | 1 | 1 | 117 | 1 | 114 | 3.23e-54 | 174 |
| MS.gene76816.t1 | AT5G67420 | 38.095 | 252 | 114 | 4 | 1 | 212 | 1 | 250 | 3.51e-53 | 171 |
| MS.gene76816.t1 | AT5G67420 | 38.095 | 252 | 114 | 4 | 1 | 212 | 1 | 250 | 3.51e-53 | 171 |
| MS.gene76816.t1 | AT4G37540 | 60.902 | 133 | 48 | 2 | 1 | 130 | 1 | 132 | 4.38e-51 | 166 |
| MS.gene76816.t1 | AT3G02550 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 1.09e-46 | 155 |
| MS.gene76816.t1 | AT1G67100 | 60.000 | 105 | 42 | 0 | 1 | 105 | 3 | 107 | 3.56e-44 | 147 |
| MS.gene76816.t1 | AT1G68510 | 47.328 | 131 | 66 | 1 | 1 | 131 | 3 | 130 | 1.10e-40 | 139 |
Find 52 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACAGCAAGAGTTTATTT+TGG | 0.186602 | 1.1:+76543201 | MS.gene76816:CDS |
| AGCCCAACTGCTCCATTTAC+TGG | 0.215944 | 1.1:-76543037 | None:intergenic |
| TTTATGACATACATGATCTT+TGG | 0.296751 | 1.1:-76542808 | None:intergenic |
| AGAACTATAAATCCAGTAAA+TGG | 0.325401 | 1.1:+76543025 | MS.gene76816:CDS |
| ATGGAGCAGTTGGGCTGTTA+TGG | 0.333353 | 1.1:+76543044 | MS.gene76816:CDS |
| GTCTTTGTCACCAAGTTCTT+CGG | 0.371969 | 1.1:+76542743 | MS.gene76816:CDS |
| TGTAATGAAGAAGCACAATC+TGG | 0.379276 | 1.1:+76543232 | MS.gene76816:CDS |
| GACATACATGATCTTTGGTT+GGG | 0.427328 | 1.1:-76542803 | None:intergenic |
| TCCACATCTAACAACTGATC+AGG | 0.449486 | 1.1:-76543145 | None:intergenic |
| CACTTATGTATGAAGCTGTT+GGG | 0.452221 | 1.1:+76543002 | MS.gene76816:CDS |
| TGGCGTTAGCCTGTGCTTGT+GGG | 0.452316 | 1.1:-76542718 | None:intergenic |
| GACACATGAGTCTGAGGAAT+CGG | 0.458754 | 1.1:+76543321 | MS.gene76816:CDS |
| GGTGGCACGGCCGAAGAACT+TGG | 0.467396 | 1.1:-76542753 | None:intergenic |
| AATCCAGTAAATGGAGCAGT+TGG | 0.467406 | 1.1:+76543034 | MS.gene76816:CDS |
| GAATCGGAGTCGTCCACGTT+GGG | 0.467550 | 1.1:+76543337 | MS.gene76816:CDS |
| GTGGCGTTAGCCTGTGCTTG+TGG | 0.478831 | 1.1:-76542719 | None:intergenic |
| TGACATACATGATCTTTGGT+TGG | 0.489850 | 1.1:-76542804 | None:intergenic |
| CCAAAATAAACTCTTGCTGT+TGG | 0.526318 | 1.1:-76543201 | None:intergenic |
| CCCTGATCAGTTGTTAGATG+TGG | 0.527616 | 1.1:+76543144 | MS.gene76816:CDS |
| ATCCAGTAAATGGAGCAGTT+GGG | 0.536591 | 1.1:+76543035 | MS.gene76816:CDS |
| TCACTTATGTATGAAGCTGT+TGG | 0.539852 | 1.1:+76543001 | MS.gene76816:CDS |
| TGCATCCCTTTCTCAAAACT+CGG | 0.542491 | 1.1:-76542652 | None:intergenic |
| GAACAAGTGCTGAGAGGTAA+CGG | 0.548928 | 1.1:+76543100 | MS.gene76816:CDS |
| ATGCTAATTCTCAAACTCAA+AGG | 0.552063 | 1.1:+76543395 | MS.gene76816:CDS |
| GAGATCATAAGAATAGTGTA+AGG | 0.555999 | 1.1:-76543426 | None:intergenic |
| GATACAAAACCCACAAGCAC+AGG | 0.556238 | 1.1:+76542709 | MS.gene76816:CDS |
| GTGCTGAGAGGTAACGGCGG+CGG | 0.556951 | 1.1:+76543106 | MS.gene76816:CDS |
| TATAATAAAATGAGTTGCAA+CGG | 0.559441 | 1.1:+76542626 | None:intergenic |
| TTTGAGAAAGGGATGCAGTG+AGG | 0.560200 | 1.1:+76542658 | MS.gene76816:CDS |
| ATAAGAATAGTGTAAGGAGT+TGG | 0.568732 | 1.1:-76543420 | None:intergenic |
| CAGCTTCATACATAAGTGAC+TGG | 0.569517 | 1.1:-76542997 | None:intergenic |
| GTAATGAAGAAGCACAATCT+GGG | 0.578649 | 1.1:+76543233 | MS.gene76816:CDS |
| ATACATGATCTTTGGTTGGG+AGG | 0.585857 | 1.1:-76542800 | None:intergenic |
| AGTACTCTAGAGTGGTGAGA+AGG | 0.591970 | 1.1:+76543266 | MS.gene76816:CDS |
| GGCTGTTATGGACTGGAAAG+TGG | 0.603769 | 1.1:+76543056 | MS.gene76816:CDS |
| GAAAAGACATGAGGGTGGCA+CGG | 0.613200 | 1.1:-76542766 | None:intergenic |
| GCAGTTGGGCTGTTATGGAC+TGG | 0.615948 | 1.1:+76543049 | MS.gene76816:CDS |
| CCACATCTAACAACTGATCA+GGG | 0.626816 | 1.1:-76543144 | None:intergenic |
| CAAGTGAAAGAAAAGACATG+AGG | 0.628292 | 1.1:-76542775 | None:intergenic |
| GATTTCACAGTACTCTAGAG+TGG | 0.637833 | 1.1:+76543258 | MS.gene76816:CDS |
| TGCGGAGACACATGAGTCTG+AGG | 0.638167 | 1.1:+76543315 | MS.gene76816:CDS |
| GGAATCGGAGTCGTCCACGT+TGG | 0.646426 | 1.1:+76543336 | MS.gene76816:CDS |
| AAGTGAAAGAAAAGACATGA+GGG | 0.649406 | 1.1:-76542774 | None:intergenic |
| AAGTGGCAATTCTGTCAACT+TGG | 0.652925 | 1.1:+76543073 | MS.gene76816:CDS |
| CCAAGCAGAAAAGCAACGTG+CGG | 0.655633 | 1.1:+76543297 | MS.gene76816:CDS |
| TTCTGTTCTACTTCCCAACG+TGG | 0.659625 | 1.1:-76543350 | None:intergenic |
| TAACAACTGATCAGGGAGTG+CGG | 0.665187 | 1.1:-76543137 | None:intergenic |
| TGCTACGAGATTGTCTAACG+TGG | 0.685370 | 1.1:+76542687 | MS.gene76816:CDS |
| TGAAAGAAAAGACATGAGGG+TGG | 0.706469 | 1.1:-76542771 | None:intergenic |
| GAACTTGGTGACAAAGACAG+TGG | 0.712149 | 1.1:-76542738 | None:intergenic |
| GGTGTTGAACAAGTGCTGAG+AGG | 0.756300 | 1.1:+76543094 | MS.gene76816:CDS |
| CAAGTGCTGAGAGGTAACGG+CGG | 0.764348 | 1.1:+76543103 | MS.gene76816:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAATTACTCTATATTAC+AGG | + | chr1.1:76542968-76542987 | MS.gene76816:intron | 20.0% |
| !!! | ACAAATTAGTTTGATGTATT+TGG | - | chr1.1:76542888-76542907 | None:intergenic | 20.0% |
| !!! | TCAAACTAATTTGTATGTAT+AGG | + | chr1.1:76542894-76542913 | MS.gene76816:intron | 20.0% |
| ! | AGAACTATAAATCCAGTAAA+TGG | + | chr1.1:76543025-76543044 | MS.gene76816:CDS | 25.0% |
| ! | TTTATGACATACATGATCTT+TGG | - | chr1.1:76542811-76542830 | None:intergenic | 25.0% |
| AAGTGAAAGAAAAGACATGA+GGG | - | chr1.1:76542777-76542796 | None:intergenic | 30.0% | |
| ATAAGAATAGTGTAAGGAGT+TGG | - | chr1.1:76543423-76543442 | None:intergenic | 30.0% | |
| ATGCTAATTCTCAAACTCAA+AGG | + | chr1.1:76543395-76543414 | MS.gene76816:CDS | 30.0% | |
| !! | CAACAGCAAGAGTTTATTTT+GGG | + | chr1.1:76543202-76543221 | MS.gene76816:CDS | 30.0% |
| CAAGTGAAAGAAAAGACATG+AGG | - | chr1.1:76542778-76542797 | None:intergenic | 35.0% | |
| CACTTATGTATGAAGCTGTT+GGG | + | chr1.1:76543002-76543021 | MS.gene76816:CDS | 35.0% | |
| GACATACATGATCTTTGGTT+GGG | - | chr1.1:76542806-76542825 | None:intergenic | 35.0% | |
| TCACTTATGTATGAAGCTGT+TGG | + | chr1.1:76543001-76543020 | MS.gene76816:CDS | 35.0% | |
| TGACATACATGATCTTTGGT+TGG | - | chr1.1:76542807-76542826 | None:intergenic | 35.0% | |
| ! | CCAAAATAAACTCTTGCTGT+TGG | - | chr1.1:76543204-76543223 | None:intergenic | 35.0% |
| ! | CCAACAGCAAGAGTTTATTT+TGG | + | chr1.1:76543201-76543220 | MS.gene76816:CDS | 35.0% |
| ! | GTAATGAAGAAGCACAATCT+GGG | + | chr1.1:76543233-76543252 | MS.gene76816:CDS | 35.0% |
| ! | TGTAATGAAGAAGCACAATC+TGG | + | chr1.1:76543232-76543251 | MS.gene76816:CDS | 35.0% |
| AAGTGGCAATTCTGTCAACT+TGG | + | chr1.1:76543073-76543092 | MS.gene76816:CDS | 40.0% | |
| AATCCAGTAAATGGAGCAGT+TGG | + | chr1.1:76543034-76543053 | MS.gene76816:CDS | 40.0% | |
| ATACATGATCTTTGGTTGGG+AGG | - | chr1.1:76542803-76542822 | None:intergenic | 40.0% | |
| ATCCAGTAAATGGAGCAGTT+GGG | + | chr1.1:76543035-76543054 | MS.gene76816:CDS | 40.0% | |
| CAGCTTCATACATAAGTGAC+TGG | - | chr1.1:76543000-76543019 | None:intergenic | 40.0% | |
| CCACATCTAACAACTGATCA+GGG | - | chr1.1:76543147-76543166 | None:intergenic | 40.0% | |
| GTCTTTGTCACCAAGTTCTT+CGG | + | chr1.1:76542743-76542762 | MS.gene76816:CDS | 40.0% | |
| TCCACATCTAACAACTGATC+AGG | - | chr1.1:76543148-76543167 | None:intergenic | 40.0% | |
| TGAAAGAAAAGACATGAGGG+TGG | - | chr1.1:76542774-76542793 | None:intergenic | 40.0% | |
| TGCATCCCTTTCTCAAAACT+CGG | - | chr1.1:76542655-76542674 | None:intergenic | 40.0% | |
| ! | GATTTCACAGTACTCTAGAG+TGG | + | chr1.1:76543258-76543277 | MS.gene76816:CDS | 40.0% |
| CCCTGATCAGTTGTTAGATG+TGG | + | chr1.1:76543144-76543163 | MS.gene76816:CDS | 45.0% | |
| GACACATGAGTCTGAGGAAT+CGG | + | chr1.1:76543321-76543340 | MS.gene76816:CDS | 45.0% | |
| GATACAAAACCCACAAGCAC+AGG | + | chr1.1:76542709-76542728 | MS.gene76816:CDS | 45.0% | |
| TAACAACTGATCAGGGAGTG+CGG | - | chr1.1:76543140-76543159 | None:intergenic | 45.0% | |
| TGCTACGAGATTGTCTAACG+TGG | + | chr1.1:76542687-76542706 | MS.gene76816:CDS | 45.0% | |
| TTCTGTTCTACTTCCCAACG+TGG | - | chr1.1:76543353-76543372 | None:intergenic | 45.0% | |
| TTTGAGAAAGGGATGCAGTG+AGG | + | chr1.1:76542658-76542677 | MS.gene76816:CDS | 45.0% | |
| ! | GAACTTGGTGACAAAGACAG+TGG | - | chr1.1:76542741-76542760 | None:intergenic | 45.0% |
| !! | AGTACTCTAGAGTGGTGAGA+AGG | + | chr1.1:76543266-76543285 | MS.gene76816:CDS | 45.0% |
| !! | GAACAAGTGCTGAGAGGTAA+CGG | + | chr1.1:76543100-76543119 | MS.gene76816:CDS | 45.0% |
| !!! | GGTTGCCGAGTTTTGAGAAA+GGG | + | chr1.1:76542647-76542666 | MS.gene76816:CDS | 45.0% |
| AGCCCAACTGCTCCATTTAC+TGG | - | chr1.1:76543040-76543059 | None:intergenic | 50.0% | |
| ATGGAGCAGTTGGGCTGTTA+TGG | + | chr1.1:76543044-76543063 | MS.gene76816:CDS | 50.0% | |
| CCAAGCAGAAAAGCAACGTG+CGG | + | chr1.1:76543297-76543316 | MS.gene76816:CDS | 50.0% | |
| GAAAAGACATGAGGGTGGCA+CGG | - | chr1.1:76542769-76542788 | None:intergenic | 50.0% | |
| !! | GGCTGTTATGGACTGGAAAG+TGG | + | chr1.1:76543056-76543075 | MS.gene76816:CDS | 50.0% |
| !! | GGTGTTGAACAAGTGCTGAG+AGG | + | chr1.1:76543094-76543113 | MS.gene76816:CDS | 50.0% |
| !!! | CGGTTGCCGAGTTTTGAGAA+AGG | + | chr1.1:76542646-76542665 | MS.gene76816:CDS | 50.0% |
| GAATCGGAGTCGTCCACGTT+GGG | + | chr1.1:76543337-76543356 | MS.gene76816:CDS | 55.0% | |
| GCAGTTGGGCTGTTATGGAC+TGG | + | chr1.1:76543049-76543068 | MS.gene76816:CDS | 55.0% | |
| TGCGGAGACACATGAGTCTG+AGG | + | chr1.1:76543315-76543334 | MS.gene76816:CDS | 55.0% | |
| ! | TGGCGTTAGCCTGTGCTTGT+GGG | - | chr1.1:76542721-76542740 | None:intergenic | 55.0% |
| !! | CAAGTGCTGAGAGGTAACGG+CGG | + | chr1.1:76543103-76543122 | MS.gene76816:CDS | 55.0% |
| !!! | CCGCACGTTGCTTTTCTGCT+TGG | - | chr1.1:76543300-76543319 | None:intergenic | 55.0% |
| GGAATCGGAGTCGTCCACGT+TGG | + | chr1.1:76543336-76543355 | MS.gene76816:CDS | 60.0% | |
| ! | GTGGCGTTAGCCTGTGCTTG+TGG | - | chr1.1:76542722-76542741 | None:intergenic | 60.0% |
| GGTGGCACGGCCGAAGAACT+TGG | - | chr1.1:76542756-76542775 | None:intergenic | 65.0% | |
| ! | GTGCTGAGAGGTAACGGCGG+CGG | + | chr1.1:76543106-76543125 | MS.gene76816:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 76542635 | 76543444 | 76542635 | ID=MS.gene76816 |
| chr1.1 | mRNA | 76542635 | 76543444 | 76542635 | ID=MS.gene76816.t1;Parent=MS.gene76816 |
| chr1.1 | exon | 76542635 | 76542818 | 76542635 | ID=MS.gene76816.t1.exon1;Parent=MS.gene76816.t1 |
| chr1.1 | CDS | 76542635 | 76542818 | 76542635 | ID=cds.MS.gene76816.t1;Parent=MS.gene76816.t1 |
| chr1.1 | exon | 76542990 | 76543444 | 76542990 | ID=MS.gene76816.t1.exon2;Parent=MS.gene76816.t1 |
| chr1.1 | CDS | 76542990 | 76543444 | 76542990 | ID=cds.MS.gene76816.t1;Parent=MS.gene76816.t1 |
| Gene Sequence |
| Protein sequence |
