AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77106

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77106.t1 MTR_2g061450 91.264 435 35 2 14 448 1 432 0.0 800
MS.gene77106.t1 MTR_2g060620 76.190 126 30 0 318 443 61 186 2.24e-60 196
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77106.t1 AT1G74120 49.436 443 220 2 6 448 7 445 6.87e-154 444
MS.gene77106.t1 AT5G54180 29.279 222 151 5 153 370 253 472 6.64e-16 80.1

Find 106 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTCAATTTCTACTTAAT+TGG 0.095296 2.4:+32598325 MS.gene77106:CDS
GAAAGCTTGGATATGAATTT+TGG 0.127070 2.4:-32598360 None:intergenic
TGTCAGGGATCCAAGGATTT+TGG 0.150914 2.4:+32598731 MS.gene77106:CDS
CATGTTCTTCACATCTTTAC+TGG 0.159159 2.4:-32599303 None:intergenic
TAGATTGTTTCCTAGGGTTT+TGG 0.199228 2.4:+32598623 MS.gene77106:CDS
AAATTTCAAGTGGGTTTAAA+TGG 0.215029 2.4:-32598434 None:intergenic
AATCTTGAAGAAGTGAAATT+AGG 0.248181 2.4:-32598287 None:intergenic
ATTTCTACTTAATTGGGAAT+TGG 0.257535 2.4:+32598332 MS.gene77106:CDS
CAACACCATATTTCATCCTT+TGG 0.306291 2.4:-32598998 None:intergenic
AAATTCACACCCATGTATTC+AGG 0.313425 2.4:-32599038 None:intergenic
AGGTCAGGGCGGAGGTTGTC+AGG 0.314844 2.4:+32598715 MS.gene77106:CDS
TTAATCCAAAGGATGAAATA+TGG 0.315058 2.4:+32598993 MS.gene77106:CDS
TTGAAAGGAAAAGGTGCAAT+TGG 0.315697 2.4:+32599101 MS.gene77106:CDS
ACCTCAATTTCTACTTAATT+GGG 0.327209 2.4:+32598326 MS.gene77106:CDS
TTTCTACTTAATTGGGAATT+GGG 0.329864 2.4:+32598333 MS.gene77106:CDS
TTTCCCATTGCAGTTGTAAT+GGG 0.333921 2.4:+32598525 MS.gene77106:CDS
AAGTGGTTCTTACAAGAATA+AGG 0.352405 2.4:-32598047 None:intergenic
GGGTTGTTAGATTGTTTCCT+AGG 0.353615 2.4:+32598616 MS.gene77106:CDS
GAAGGAAAAGAGGGTTGTTA+AGG 0.369880 2.4:-32598247 None:intergenic
GAAGAACATGAAGGCTTTCA+TGG 0.374988 2.4:+32599316 MS.gene77106:CDS
CACAATCTTTCCAATGGGGT+TGG 0.394153 2.4:+32597993 None:intergenic
AAGGATGATTGCTTACGGTT+TGG 0.397737 2.4:+32598944 MS.gene77106:CDS
GGTGCAATTGGGTTTGAAGT+TGG 0.399622 2.4:+32599113 MS.gene77106:CDS
TTCACATCTTTACTGGTTTG+AGG 0.401092 2.4:-32599296 None:intergenic
TCACATCTTTACTGGTTTGA+GGG 0.406910 2.4:-32599295 None:intergenic
GATTCGTAGGGATGCTTTGA+AGG 0.407568 2.4:+32598905 MS.gene77106:CDS
GAAAGCATCCAGCTGGACTT+TGG 0.410449 2.4:+32599249 MS.gene77106:CDS
ACAAGTTGGCGATCATCAAC+GGG 0.415975 2.4:-32598392 None:intergenic
AGGAAGGGAAACCGAAGGTT+TGG 0.417132 2.4:-32598155 None:intergenic
ATTTCCCATTGCAGTTGTAA+TGG 0.419491 2.4:+32598524 MS.gene77106:CDS
TTCCTAAGGATGAGATTGAT+AGG 0.422720 2.4:+32598595 MS.gene77106:CDS
ACAATCTTTCCAATGGGGTT+GGG 0.423298 2.4:+32597994 None:intergenic
AAACCACTTCCTCTTCAATT+CGG 0.423347 2.4:+32598203 MS.gene77106:CDS
CATTGTATCTAGGAACAATC+TGG 0.424186 2.4:-32599067 None:intergenic
TGAAAGGAAAAGGTGCAATT+GGG 0.424464 2.4:+32599102 MS.gene77106:CDS
TGTTGCAGAGTTTGAAATGC+AGG 0.431814 2.4:+32598790 MS.gene77106:CDS
TTATAAAATCTAAGTCTAGT+TGG 0.433063 2.4:-32599155 None:intergenic
TCAAGATTGCCCTTCTGTGT+TGG 0.433382 2.4:+32598302 MS.gene77106:CDS
TTACTGGTTTGAGGGAACTT+TGG 0.436445 2.4:-32599287 None:intergenic
GCTAATCTCTTCCAAACCTT+CGG 0.450033 2.4:+32598144 MS.gene77106:CDS
ATCTCCATTCCCAAAATCCT+TGG 0.454905 2.4:-32598741 None:intergenic
TACTCAATCACATTGTATCT+AGG 0.456654 2.4:-32599077 None:intergenic
GCGGTCACGGTTTGATTCGT+AGG 0.460595 2.4:+32598892 MS.gene77106:CDS
GATGATTGCTTACGGTTTGG+AGG 0.460844 2.4:+32598947 MS.gene77106:CDS
TCCCAATTAAGTAGAAATTG+AGG 0.461386 2.4:-32598327 None:intergenic
TTGTTAAGGATTGGCGAAGG+TGG 0.462969 2.4:-32598233 None:intergenic
GTTGTTATTAGTTGAAGAAG+TGG 0.465877 2.4:-32598064 None:intergenic
CGGGGAAGCATTAGAAAGCT+TGG 0.465957 2.4:-32598373 None:intergenic
AAATGGGGAAGAGAAGAGGA+AGG 0.466733 2.4:-32598171 None:intergenic
TGTTAAGGATTGGCGAAGGT+GGG 0.467753 2.4:-32598232 None:intergenic
TATGGAATTTGGGGTTCCTA+AGG 0.471062 2.4:+32598581 MS.gene77106:CDS
ATTGAGTATTTGAAAGGAAA+AGG 0.475963 2.4:+32599092 MS.gene77106:CDS
CAGTCGAGAGGTCAGGGCGG+AGG 0.476069 2.4:+32598707 MS.gene77106:CDS
TTTGAGGGAACTTTGGTGGT+TGG 0.477860 2.4:-32599280 None:intergenic
AAAAGAGGGTTGTTAAGGAT+TGG 0.487510 2.4:-32598242 None:intergenic
GTGGATTGTTTGTGCGGTCA+CGG 0.490001 2.4:+32598879 MS.gene77106:CDS
CTGGTTTGAGGGAACTTTGG+TGG 0.495315 2.4:-32599284 None:intergenic
GGTTGTTAGATTGTTTCCTA+GGG 0.496449 2.4:+32598617 MS.gene77106:CDS
AATGTGATTGAGTATTTGAA+AGG 0.505696 2.4:+32599086 MS.gene77106:CDS
AGATGTAGGTGGATTAGAAA+TGG 0.507251 2.4:-32598097 None:intergenic
ACCCTATCAATCTCATCCTT+AGG 0.512362 2.4:-32598597 None:intergenic
AACAAGTTGGCGATCATCAA+CGG 0.516565 2.4:-32598393 None:intergenic
TGTAGGTGGATTAGAAATGG+TGG 0.521617 2.4:-32598094 None:intergenic
AACATCTTCCAAAGTCCAGC+TGG 0.524566 2.4:-32599257 None:intergenic
GATCCGAATTGAAGAGGAAG+TGG 0.526858 2.4:-32598206 None:intergenic
GAACCAAGGATGATTGCTTA+CGG 0.528687 2.4:+32598939 MS.gene77106:CDS
GCCGTTGATTCATGAGTTGA+GGG 0.529192 2.4:+32598671 MS.gene77106:CDS
GCATGAAGTTCCTGAATACA+TGG 0.531558 2.4:+32599028 MS.gene77106:CDS
AGCCGTTGATTCATGAGTTG+AGG 0.532065 2.4:+32598670 MS.gene77106:CDS
TCCTAAGGATGAGATTGATA+GGG 0.538639 2.4:+32598596 MS.gene77106:CDS
CTCCGCCCTGACCTCTCGAC+TGG 0.540014 2.4:-32598706 None:intergenic
GAGGAAATGGGGAAGAGAAG+AGG 0.540975 2.4:-32598175 None:intergenic
AAACCGTAAGCAATCATCCT+TGG 0.541022 2.4:-32598942 None:intergenic
GTTAAAAGAAAGCATCCAGC+TGG 0.543247 2.4:+32599242 MS.gene77106:CDS
CGGAGGTTGTCAGGGATCCA+AGG 0.544470 2.4:+32598724 MS.gene77106:CDS
AAGGATTGGCGAAGGTGGGA+TGG 0.547823 2.4:-32598228 None:intergenic
CGGTCACGGTTTGATTCGTA+GGG 0.549755 2.4:+32598893 MS.gene77106:CDS
ATGGTGGAACCCAACCCCAT+TGG 0.554824 2.4:-32598003 None:intergenic
CGTTGATTCATGAGTTGAGG+GGG 0.559779 2.4:+32598673 MS.gene77106:CDS
GTTGCAGAGTTTGAAATGCA+GGG 0.562878 2.4:+32598791 MS.gene77106:CDS
ATACAAAATCCCAAAACCCT+AGG 0.565652 2.4:-32598633 None:intergenic
CCCCTCAACTCATGAATCAA+CGG 0.566111 2.4:-32598672 None:intergenic
GAAGGCTTTCATGGACTCGT+TGG 0.570328 2.4:+32599325 MS.gene77106:CDS
TCCCATTGCAGTTGTAATGG+GGG 0.579132 2.4:+32598527 MS.gene77106:CDS
TGCTTTGAAGGTGTTGTGGA+AGG 0.580553 2.4:+32598917 MS.gene77106:CDS
GGTCAGGGCGGAGGTTGTCA+GGG 0.584107 2.4:+32598716 MS.gene77106:CDS
TTCCCATTGCAGTTGTAATG+GGG 0.586307 2.4:+32598526 MS.gene77106:CDS
AATTGAGGTTCCAACACAGA+AGG 0.588412 2.4:-32598312 None:intergenic
AATGGGGAAGAGAAGAGGAA+GGG 0.597700 2.4:-32598170 None:intergenic
TGTAGAACAACTCAACAAGT+TGG 0.602305 2.4:-32598406 None:intergenic
ATTGAGGTTCCAACACAGAA+GGG 0.606343 2.4:-32598311 None:intergenic
GGGTTGTTAAGGATTGGCGA+AGG 0.612411 2.4:-32598236 None:intergenic
GGGATGCTTTGAAGGTGTTG+TGG 0.613256 2.4:+32598913 MS.gene77106:CDS
TAAAGATGTGAAGAACATGA+AGG 0.617044 2.4:+32599307 MS.gene77106:CDS
AGAAGAGGAAGGGAAACCGA+AGG 0.621331 2.4:-32598160 None:intergenic
GGGATGGATCCGAATTGAAG+AGG 0.622695 2.4:-32598212 None:intergenic
CCGTTGATTCATGAGTTGAG+GGG 0.629124 2.4:+32598672 MS.gene77106:CDS
ACTTTACGGGAAATTTCAAG+TGG 0.630860 2.4:-32598444 None:intergenic
CTGAGAGTGGATTGTTTGTG+CGG 0.636549 2.4:+32598873 MS.gene77106:CDS
CTTTACGGGAAATTTCAAGT+GGG 0.640015 2.4:-32598443 None:intergenic
TTTCTGTAATGAGATGTAGG+TGG 0.659285 2.4:-32598108 None:intergenic
CATGAAGTTCCTGAATACAT+GGG 0.707620 2.4:+32599029 MS.gene77106:CDS
AGGTGTTGTGGAAGGAACCA+AGG 0.715844 2.4:+32598925 MS.gene77106:CDS
TTCCAGTCGAGAGGTCAGGG+CGG 0.718711 2.4:+32598704 MS.gene77106:CDS
GCAGAGTTTGAAATGCAGGG+AGG 0.745889 2.4:+32598794 MS.gene77106:CDS
CAAGTTGGCGATCATCAACG+GGG 0.759642 2.4:-32598391 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTCTTATGGAATTT+GGG + chr2.4:32598571-32598590 MS.gene77106:CDS 15.0%
!! TTATAAAATCTAAGTCTAGT+TGG - chr2.4:32599158-32599177 None:intergenic 20.0%
!!! AATTAAAGAGAAGATTTTTG+GGG + chr2.4:32598818-32598837 MS.gene77106:CDS 20.0%
!!! CAATTAAAGAGAAGATTTTT+GGG + chr2.4:32598817-32598836 MS.gene77106:CDS 20.0%
!!! CATATATTTTTTCACATTCA+GGG - chr2.4:32599199-32599218 None:intergenic 20.0%
!!! GTTAATTTTCTTATGGAATT+TGG + chr2.4:32598570-32598589 MS.gene77106:CDS 20.0%
!!! TAATTTTCTTATGGAATTTG+GGG + chr2.4:32598572-32598591 MS.gene77106:CDS 20.0%
! AACCTCAATTTCTACTTAAT+TGG + chr2.4:32598325-32598344 MS.gene77106:CDS 25.0%
! AATCTTGAAGAAGTGAAATT+AGG - chr2.4:32598290-32598309 None:intergenic 25.0%
! AATGTGATTGAGTATTTGAA+AGG + chr2.4:32599086-32599105 MS.gene77106:CDS 25.0%
! ACCTCAATTTCTACTTAATT+GGG + chr2.4:32598326-32598345 MS.gene77106:CDS 25.0%
! ATTGAGTATTTGAAAGGAAA+AGG + chr2.4:32599092-32599111 MS.gene77106:CDS 25.0%
! ATTTCTACTTAATTGGGAAT+TGG + chr2.4:32598332-32598351 MS.gene77106:CDS 25.0%
! CCCTAAAAATTTCAACTTTA+CGG - chr2.4:32598461-32598480 None:intergenic 25.0%
! CCTAAAAATTTCAACTTTAC+GGG - chr2.4:32598460-32598479 None:intergenic 25.0%
! CCTGAATGTGAAAAAATATA+TGG + chr2.4:32599197-32599216 MS.gene77106:CDS 25.0%
! TTAATCCAAAGGATGAAATA+TGG + chr2.4:32598993-32599012 MS.gene77106:CDS 25.0%
! TTTCTACTTAATTGGGAATT+GGG + chr2.4:32598333-32598352 MS.gene77106:CDS 25.0%
!! AAATTTCAAGTGGGTTTAAA+TGG - chr2.4:32598437-32598456 None:intergenic 25.0%
!! AGATTGAGTTTTTAATCCAA+AGG + chr2.4:32598982-32599001 MS.gene77106:CDS 25.0%
!! CCGTAAAGTTGAAATTTTTA+GGG + chr2.4:32598458-32598477 MS.gene77106:CDS 25.0%
!! GCAATTAAAGAGAAGATTTT+TGG + chr2.4:32598816-32598835 MS.gene77106:CDS 25.0%
!!! CCATATATTTTTTCACATTC+AGG - chr2.4:32599200-32599219 None:intergenic 25.0%
!!! CGTAAAGTTGAAATTTTTAG+GGG + chr2.4:32598459-32598478 MS.gene77106:CDS 25.0%
!!! TTCTGATGATGTTTTGTTAA+GGG + chr2.4:32598491-32598510 MS.gene77106:CDS 25.0%
!!! TTTCTGATGATGTTTTGTTA+AGG + chr2.4:32598490-32598509 MS.gene77106:CDS 25.0%
AAGTGGTTCTTACAAGAATA+AGG - chr2.4:32598050-32598069 None:intergenic 30.0%
TAAAGATGTGAAGAACATGA+AGG + chr2.4:32599307-32599326 MS.gene77106:CDS 30.0%
TACTCAATCACATTGTATCT+AGG - chr2.4:32599080-32599099 None:intergenic 30.0%
TCCCAATTAAGTAGAAATTG+AGG - chr2.4:32598330-32598349 None:intergenic 30.0%
! AGTGAAATTAGGTTTTTCTG+TGG - chr2.4:32598279-32598298 None:intergenic 30.0%
! ATATGGCAGATTTTCAGAAA+AGG + chr2.4:32599214-32599233 MS.gene77106:CDS 30.0%
! CCCGTAAAGTTGAAATTTTT+AGG + chr2.4:32598457-32598476 MS.gene77106:CDS 30.0%
! GAAAGCTTGGATATGAATTT+TGG - chr2.4:32598363-32598382 None:intergenic 30.0%
! GTTGTTATTAGTTGAAGAAG+TGG - chr2.4:32598067-32598086 None:intergenic 30.0%
! GTTTTTCTGTGGAATTTTGA+AGG - chr2.4:32598268-32598287 None:intergenic 30.0%
! TGTTTTCTGTAATGAGATGT+AGG - chr2.4:32598114-32598133 None:intergenic 30.0%
!! GTTGAAATTTTTAGGGGTTT+AGG + chr2.4:32598465-32598484 MS.gene77106:CDS 30.0%
!! TGATGTTTTGTTAAGGGTTT+TGG + chr2.4:32598497-32598516 MS.gene77106:CDS 30.0%
!! TGGAATTTTGAAGGAAAAGA+GGG - chr2.4:32598259-32598278 None:intergenic 30.0%
!!! ATTTTTTCACATTCAGGGTA+AGG - chr2.4:32599194-32599213 None:intergenic 30.0%
!!! GTTGTTTCTTTTTCTGACTA+TGG - chr2.4:32598025-32598044 None:intergenic 30.0%
!!! TGGTGTCGTTAATTTTCTTA+TGG + chr2.4:32598563-32598582 MS.gene77106:CDS 30.0%
AAAAGAGGGTTGTTAAGGAT+TGG - chr2.4:32598245-32598264 None:intergenic 35.0%
AAACCACTTCCTCTTCAATT+CGG + chr2.4:32598203-32598222 MS.gene77106:CDS 35.0%
AAATTCACACCCATGTATTC+AGG - chr2.4:32599041-32599060 None:intergenic 35.0%
ACTTTACGGGAAATTTCAAG+TGG - chr2.4:32598447-32598466 None:intergenic 35.0%
ATACAAAATCCCAAAACCCT+AGG - chr2.4:32598636-32598655 None:intergenic 35.0%
ATTTCCCATTGCAGTTGTAA+TGG + chr2.4:32598524-32598543 MS.gene77106:CDS 35.0%
CAACACCATATTTCATCCTT+TGG - chr2.4:32599001-32599020 None:intergenic 35.0%
CATGAAGTTCCTGAATACAT+GGG + chr2.4:32599029-32599048 MS.gene77106:CDS 35.0%
CATGTTCTTCACATCTTTAC+TGG - chr2.4:32599306-32599325 None:intergenic 35.0%
CATTGTATCTAGGAACAATC+TGG - chr2.4:32599070-32599089 None:intergenic 35.0%
GGTTGTTAGATTGTTTCCTA+GGG + chr2.4:32598617-32598636 MS.gene77106:CDS 35.0%
TCCTAAGGATGAGATTGATA+GGG + chr2.4:32598596-32598615 MS.gene77106:CDS 35.0%
TGTAGAACAACTCAACAAGT+TGG - chr2.4:32598409-32598428 None:intergenic 35.0%
TTCCTAAGGATGAGATTGAT+AGG + chr2.4:32598595-32598614 MS.gene77106:CDS 35.0%
TTTCCCATTGCAGTTGTAAT+GGG + chr2.4:32598525-32598544 MS.gene77106:CDS 35.0%
TTTCTGTAATGAGATGTAGG+TGG - chr2.4:32598111-32598130 None:intergenic 35.0%
! AGATTGTTTCCTAGGGTTTT+GGG + chr2.4:32598624-32598643 MS.gene77106:CDS 35.0%
! ATTGAAGAGGAAGTGGTTTT+TGG - chr2.4:32598202-32598221 None:intergenic 35.0%
! CTTTACGGGAAATTTCAAGT+GGG - chr2.4:32598446-32598465 None:intergenic 35.0%
! GTTTCTTTTTCTGACTATGG+TGG - chr2.4:32598022-32598041 None:intergenic 35.0%
! TAGATTGTTTCCTAGGGTTT+TGG + chr2.4:32598623-32598642 MS.gene77106:CDS 35.0%
! TGAAAGGAAAAGGTGCAATT+GGG + chr2.4:32599102-32599121 MS.gene77106:CDS 35.0%
! TGGGATTTTGTATTGAGGAT+AGG + chr2.4:32598643-32598662 MS.gene77106:CDS 35.0%
! TTGAAAGGAAAAGGTGCAAT+TGG + chr2.4:32599101-32599120 MS.gene77106:CDS 35.0%
!! AAAGAGAAGATTTTTGGGGA+GGG + chr2.4:32598822-32598841 MS.gene77106:CDS 35.0%
!! AGATGTAGGTGGATTAGAAA+TGG - chr2.4:32598100-32598119 None:intergenic 35.0%
!! GTGGAATTTTGAAGGAAAAG+AGG - chr2.4:32598260-32598279 None:intergenic 35.0%
!! TAAAGAGAAGATTTTTGGGG+AGG + chr2.4:32598821-32598840 MS.gene77106:CDS 35.0%
!! TCACATCTTTACTGGTTTGA+GGG - chr2.4:32599298-32599317 None:intergenic 35.0%
!! TGTTTTGTTAAGGGTTTTGG+AGG + chr2.4:32598500-32598519 MS.gene77106:CDS 35.0%
!! TTCACATCTTTACTGGTTTG+AGG - chr2.4:32599299-32599318 None:intergenic 35.0%
!!! GGTTTTGGGATTTTGTATTG+AGG + chr2.4:32598638-32598657 MS.gene77106:CDS 35.0%
AAACCGTAAGCAATCATCCT+TGG - chr2.4:32598945-32598964 None:intergenic 40.0%
AAGGATGATTGCTTACGGTT+TGG + chr2.4:32598944-32598963 MS.gene77106:CDS 40.0%
AATTGAGGTTCCAACACAGA+AGG - chr2.4:32598315-32598334 None:intergenic 40.0%
ACCCTATCAATCTCATCCTT+AGG - chr2.4:32598600-32598619 None:intergenic 40.0%
ATCTCCATTCCCAAAATCCT+TGG - chr2.4:32598744-32598763 None:intergenic 40.0%
ATGGGGGAAACTCAAATTGT+TGG + chr2.4:32598543-32598562 MS.gene77106:CDS 40.0%
ATTGAGGTTCCAACACAGAA+GGG - chr2.4:32598314-32598333 None:intergenic 40.0%
GAACCAAGGATGATTGCTTA+CGG + chr2.4:32598939-32598958 MS.gene77106:CDS 40.0%
GAAGAACATGAAGGCTTTCA+TGG + chr2.4:32599316-32599335 MS.gene77106:CDS 40.0%
GAAGGAAAAGAGGGTTGTTA+AGG - chr2.4:32598250-32598269 None:intergenic 40.0%
GCATGAAGTTCCTGAATACA+TGG + chr2.4:32599028-32599047 MS.gene77106:CDS 40.0%
GCTAATCTCTTCCAAACCTT+CGG + chr2.4:32598144-32598163 MS.gene77106:CDS 40.0%
GGGTTGTTAGATTGTTTCCT+AGG + chr2.4:32598616-32598635 MS.gene77106:CDS 40.0%
TTCCCATTGCAGTTGTAATG+GGG + chr2.4:32598526-32598545 MS.gene77106:CDS 40.0%
! AACAAGTTGGCGATCATCAA+CGG - chr2.4:32598396-32598415 None:intergenic 40.0%
! GTTGCAGAGTTTGAAATGCA+GGG + chr2.4:32598791-32598810 MS.gene77106:CDS 40.0%
! TATGGAATTTGGGGTTCCTA+AGG + chr2.4:32598581-32598600 MS.gene77106:CDS 40.0%
! TGGAGATTGGGGAATTTTCA+CGG + chr2.4:32598757-32598776 MS.gene77106:CDS 40.0%
! TGTTGCAGAGTTTGAAATGC+AGG + chr2.4:32598790-32598809 MS.gene77106:CDS 40.0%
!! AGGATTTTGGGAATGGAGAT+TGG + chr2.4:32598744-32598763 MS.gene77106:CDS 40.0%
!! AGTGGTTTTTGGAGAGGAAA+TGG - chr2.4:32598191-32598210 None:intergenic 40.0%
!! GATTTTGGGAATGGAGATTG+GGG + chr2.4:32598746-32598765 MS.gene77106:CDS 40.0%
!! GGATTTTGGGAATGGAGATT+GGG + chr2.4:32598745-32598764 MS.gene77106:CDS 40.0%
!! GTGGTTTTTGGAGAGGAAAT+GGG - chr2.4:32598190-32598209 None:intergenic 40.0%
!! GTTAAAAGAAAGCATCCAGC+TGG + chr2.4:32599242-32599261 MS.gene77106:CDS 40.0%
!! TGGTTTTTGGAGAGGAAATG+GGG - chr2.4:32598189-32598208 None:intergenic 40.0%
!! TGTAGGTGGATTAGAAATGG+TGG - chr2.4:32598097-32598116 None:intergenic 40.0%
!! TTACTGGTTTGAGGGAACTT+TGG - chr2.4:32599290-32599309 None:intergenic 40.0%
!!! TTTTGAAGTGAAGCTGAGAG+TGG + chr2.4:32598860-32598879 MS.gene77106:CDS 40.0%
AAATGGGGAAGAGAAGAGGA+AGG - chr2.4:32598174-32598193 None:intergenic 45.0%
AACATCTTCCAAAGTCCAGC+TGG - chr2.4:32599260-32599279 None:intergenic 45.0%
AATGGGGAAGAGAAGAGGAA+GGG - chr2.4:32598173-32598192 None:intergenic 45.0%
CCCCTCAACTCATGAATCAA+CGG - chr2.4:32598675-32598694 None:intergenic 45.0%
GATCCGAATTGAAGAGGAAG+TGG - chr2.4:32598209-32598228 None:intergenic 45.0%
GATTCGTAGGGATGCTTTGA+AGG + chr2.4:32598905-32598924 MS.gene77106:CDS 45.0%
TCAAGATTGCCCTTCTGTGT+TGG + chr2.4:32598302-32598321 MS.gene77106:CDS 45.0%
TCCCATTGCAGTTGTAATGG+GGG + chr2.4:32598527-32598546 MS.gene77106:CDS 45.0%
TCCCCCATTACAACTGCAAT+GGG - chr2.4:32598531-32598550 None:intergenic 45.0%
TTCCCCCATTACAACTGCAA+TGG - chr2.4:32598532-32598551 None:intergenic 45.0%
! ACAAGTTGGCGATCATCAAC+GGG - chr2.4:32598395-32598414 None:intergenic 45.0%
! ATTCATGAGTTGAGGGGGTT+GGG + chr2.4:32598678-32598697 MS.gene77106:CDS 45.0%
! GATGATTGCTTACGGTTTGG+AGG + chr2.4:32598947-32598966 MS.gene77106:CDS 45.0%
! GGTGCAATTGGGTTTGAAGT+TGG + chr2.4:32599113-32599132 MS.gene77106:CDS 45.0%
! GTCAGGGATCCAAGGATTTT+GGG + chr2.4:32598732-32598751 MS.gene77106:CDS 45.0%
! TGTCAGGGATCCAAGGATTT+TGG + chr2.4:32598731-32598750 MS.gene77106:CDS 45.0%
! TGTTAAGGATTGGCGAAGGT+GGG - chr2.4:32598235-32598254 None:intergenic 45.0%
! TTGTTAAGGATTGGCGAAGG+TGG - chr2.4:32598236-32598255 None:intergenic 45.0%
! TTTGAGGGAACTTTGGTGGT+TGG - chr2.4:32599283-32599302 None:intergenic 45.0%
!! AGAGGAAGTGGTTTTTGGAG+AGG - chr2.4:32598197-32598216 None:intergenic 45.0%
!! AGCCGTTGATTCATGAGTTG+AGG + chr2.4:32598670-32598689 MS.gene77106:CDS 45.0%
!! CCGTTGATTCATGAGTTGAG+GGG + chr2.4:32598672-32598691 MS.gene77106:CDS 45.0%
!! CGTTGATTCATGAGTTGAGG+GGG + chr2.4:32598673-32598692 MS.gene77106:CDS 45.0%
!! CTGAGAGTGGATTGTTTGTG+CGG + chr2.4:32598873-32598892 MS.gene77106:CDS 45.0%
!! GAAGATTTTTGGGGAGGGTT+TGG + chr2.4:32598827-32598846 MS.gene77106:CDS 45.0%
!! GCCGTTGATTCATGAGTTGA+GGG + chr2.4:32598671-32598690 MS.gene77106:CDS 45.0%
!! GGATCCAAGGATTTTGGGAA+TGG + chr2.4:32598737-32598756 MS.gene77106:CDS 45.0%
!! TGCTTTGAAGGTGTTGTGGA+AGG + chr2.4:32598917-32598936 MS.gene77106:CDS 45.0%
!! TTTTGGGGAGGGTTTGGTTA+GGG + chr2.4:32598833-32598852 MS.gene77106:CDS 45.0%
!! TTTTTGGGGAGGGTTTGGTT+AGG + chr2.4:32598832-32598851 MS.gene77106:CDS 45.0%
AGAAGAGGAAGGGAAACCGA+AGG - chr2.4:32598163-32598182 None:intergenic 50.0%
AGGAAGGGAAACCGAAGGTT+TGG - chr2.4:32598158-32598177 None:intergenic 50.0%
GAAGGCTTTCATGGACTCGT+TGG + chr2.4:32599325-32599344 MS.gene77106:CDS 50.0%
GAGGAAATGGGGAAGAGAAG+AGG - chr2.4:32598178-32598197 None:intergenic 50.0%
GGGATGGATCCGAATTGAAG+AGG - chr2.4:32598215-32598234 None:intergenic 50.0%
! AGGTGTTGTGGAAGGAACCA+AGG + chr2.4:32598925-32598944 MS.gene77106:CDS 50.0%
! CAAGTTGGCGATCATCAACG+GGG - chr2.4:32598394-32598413 None:intergenic 50.0%
! CGGTCACGGTTTGATTCGTA+GGG + chr2.4:32598893-32598912 MS.gene77106:CDS 50.0%
! CTGGTTTGAGGGAACTTTGG+TGG - chr2.4:32599287-32599306 None:intergenic 50.0%
! GATTCATGAGTTGAGGGGGT+TGG + chr2.4:32598677-32598696 MS.gene77106:CDS 50.0%
! GCAGAGTTTGAAATGCAGGG+AGG + chr2.4:32598794-32598813 MS.gene77106:CDS 50.0%
! GGGTTGTTAAGGATTGGCGA+AGG - chr2.4:32598239-32598258 None:intergenic 50.0%
! GTGGATTGTTTGTGCGGTCA+CGG + chr2.4:32598879-32598898 MS.gene77106:CDS 50.0%
! GTTTTCCAGTCGAGAGGTCA+GGG + chr2.4:32598701-32598720 MS.gene77106:CDS 50.0%
! TTCATGAGTTGAGGGGGTTG+GGG + chr2.4:32598679-32598698 MS.gene77106:CDS 50.0%
!! CGGGGAAGCATTAGAAAGCT+TGG - chr2.4:32598376-32598395 None:intergenic 50.0%
!! GAAAGCATCCAGCTGGACTT+TGG + chr2.4:32599249-32599268 MS.gene77106:CDS 50.0%
!! GGGATGCTTTGAAGGTGTTG+TGG + chr2.4:32598913-32598932 MS.gene77106:CDS 50.0%
! AAGGATTGGCGAAGGTGGGA+TGG - chr2.4:32598231-32598250 None:intergenic 55.0%
! GCGGTCACGGTTTGATTCGT+AGG + chr2.4:32598892-32598911 MS.gene77106:CDS 55.0%
! GGTTTTCCAGTCGAGAGGTC+AGG + chr2.4:32598700-32598719 MS.gene77106:CDS 55.0%
! GTTGGGGTTTTCCAGTCGAG+AGG + chr2.4:32598695-32598714 MS.gene77106:CDS 55.0%
CGGAGGTTGTCAGGGATCCA+AGG + chr2.4:32598724-32598743 MS.gene77106:CDS 60.0%
TTCCAGTCGAGAGGTCAGGG+CGG + chr2.4:32598704-32598723 MS.gene77106:CDS 60.0%
AGGTCAGGGCGGAGGTTGTC+AGG + chr2.4:32598715-32598734 MS.gene77106:CDS 65.0%
GGTCAGGGCGGAGGTTGTCA+GGG + chr2.4:32598716-32598735 MS.gene77106:CDS 65.0%
CAGTCGAGAGGTCAGGGCGG+AGG + chr2.4:32598707-32598726 MS.gene77106:CDS 70.0%
CTCCGCCCTGACCTCTCGAC+TGG - chr2.4:32598709-32598728 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32598006 32599352 32598006 ID=MS.gene77106
chr2.4 mRNA 32598006 32599352 32598006 ID=MS.gene77106.t1;Parent=MS.gene77106
chr2.4 exon 32598006 32599352 32598006 ID=MS.gene77106.t1.exon1;Parent=MS.gene77106.t1
chr2.4 CDS 32598006 32599352 32598006 ID=cds.MS.gene77106.t1;Parent=MS.gene77106.t1
Gene Sequence

>MS.gene77106

ATGGGGTTGGGTTCCACCATAGTCAGAAAAAGAAACAACGTCCTTATTCTTGTAAGAACCACTTCTTCAACTAATAACAACTACTTCTCCACCATTTCTAATCCACCTACATCTCATTACAGAAAACAAATCGCACTCGCTAATCTCTTCCAAACCTTCGGTTTCCCTTCCTCTTCTCTTCCCCATTTCCTCTCCAAAAACCACTTCCTCTTCAATTCGGATCCATCCCACCTTCGCCAATCCTTAACAACCCTCTTTTCCTTCAAAATTCCACAGAAAAACCTAATTTCACTTCTTCAAGATTGCCCTTCTGTGTTGGAACCTCAATTTCTACTTAATTGGGAATTGGGTTTTCCAAAATTCATATCCAAGCTTTCTAATGCTTCCCCGTTGATGATCGCCAACTTGTTGAGTTGTTCTACAACTTTCCATTTAAACCCACTTGAAATTTCCCGTAAAGTTGAAATTTTTAGGGGTTTAGGATTTTCTGATGATGTTTTGTTAAGGGTTTTGGAGGAATTTCCCATTGCAGTTGTAATGGGGGAAACTCAAATTGTTGGTGTCGTTAATTTTCTTATGGAATTTGGGGTTCCTAAGGATGAGATTGATAGGGTTGTTAGATTGTTTCCTAGGGTTTTGGGATTTTGTATTGAGGATAGGTTGAAGCCGTTGATTCATGAGTTGAGGGGGTTGGGGTTTTCCAGTCGAGAGGTCAGGGCGGAGGTTGTCAGGGATCCAAGGATTTTGGGAATGGAGATTGGGGAATTTTCACGGTGTTTGAAGTTGTTGCAGAGTTTGAAATGCAGGGAGGCAATTAAAGAGAAGATTTTTGGGGAGGGTTTGGTTAGGGCATGTTTTGAAGTGAAGCTGAGAGTGGATTGTTTGTGCGGTCACGGTTTGATTCGTAGGGATGCTTTGAAGGTGTTGTGGAAGGAACCAAGGATGATTGCTTACGGTTTGGAGGATATTGAGAAGAAGATTGAGTTTTTAATCCAAAGGATGAAATATGGTGTTGATTGTTTGCATGAAGTTCCTGAATACATGGGTGTGAATTTTGAAAACCAGATTGTTCCTAGATACAATGTGATTGAGTATTTGAAAGGAAAAGGTGCAATTGGGTTTGAAGTTGGATTGAAAGACATTATCAAACCAACTAGACTTAGATTTTATAATCTTTATGTCAAGCCTTACCCTGAATGTGAAAAAATATATGGCAGATTTTCAGAAAAGGTTGAAGTTAAAAGAAAGCATCCAGCTGGACTTTGGAAGATGTTCCAACCACCAAAGTTCCCTCAAACCAGTAAAGATGTGAAGAACATGAAGGCTTTCATGGACTCGTTGGTGTAG
Protein sequence

>MS.gene77106.t1

MGLGSTIVRKRNNVLILVRTTSSTNNNYFSTISNPPTSHYRKQIALANLFQTFGFPSSSLPHFLSKNHFLFNSDPSHLRQSLTTLFSFKIPQKNLISLLQDCPSVLEPQFLLNWELGFPKFISKLSNASPLMIANLLSCSTTFHLNPLEISRKVEIFRGLGFSDDVLLRVLEEFPIAVVMGETQIVGVVNFLMEFGVPKDEIDRVVRLFPRVLGFCIEDRLKPLIHELRGLGFSSREVRAEVVRDPRILGMEIGEFSRCLKLLQSLKCREAIKEKIFGEGLVRACFEVKLRVDCLCGHGLIRRDALKVLWKEPRMIAYGLEDIEKKIEFLIQRMKYGVDCLHEVPEYMGVNFENQIVPRYNVIEYLKGKGAIGFEVGLKDIIKPTRLRFYNLYVKPYPECEKIYGRFSEKVEVKRKHPAGLWKMFQPPKFPQTSKDVKNMKAFMDSLV