AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77117

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 124 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTATGTGGAGGCGGATTT+TGG 0.148513 2.4:-32492830 None:intergenic
TTCCTCCGGCGGCATTTCTC+AGG 0.275496 2.4:+32493319 MS.gene77117:CDS
AGATTTGTTGCACGAGTGTT+TGG 0.301888 2.4:+32493210 MS.gene77117:CDS
CCTGATTGCTGCCGAGATAC+TGG 0.331416 2.4:+32492584 MS.gene77117:CDS
TTCGGGATGAGGAGGAGAAT+TGG 0.336432 2.4:-32492766 None:intergenic
ACTCGTGCAACAAATCTTCT+TGG 0.342234 2.4:-32493204 None:intergenic
GGTGGGAATGTGGCTAATAA+TGG 0.350488 2.4:+32493014 MS.gene77117:CDS
GTATAGGCTAAGGAGTTTGC+TGG 0.351839 2.4:+32492425 MS.gene77117:CDS
TAAGACACACTTACCTTGTC+TGG 0.362065 2.4:-32491599 None:intergenic
TTCAACTCCTGTGGCAGAAC+CGG 0.369949 2.4:+32492719 MS.gene77117:CDS
TCCCTAGCATACATAACATA+TGG 0.379144 2.4:-32493420 None:intergenic
GACACAAGAGGTTCGAGGTT+TGG 0.386987 2.4:+32492366 MS.gene77117:CDS
TCATGGCCGCGCTTACATTC+AGG 0.392786 2.4:+32491555 None:intergenic
TGGCAGTTGGGAAAAGTGTT+AGG 0.395161 2.4:+32493376 MS.gene77117:CDS
GGCATTGTGTGGTTGTTGAT+TGG 0.403874 2.4:-32492799 None:intergenic
TGATTGGCATCATGAACTTC+GGG 0.411728 2.4:-32492783 None:intergenic
CGGAGAACCGGCTGGCAGTT+GGG 0.421662 2.4:+32493364 MS.gene77117:CDS
ACTCCTTAGCCTATACAAAA+AGG 0.422371 2.4:-32492418 None:intergenic
TGATGCTGCTGCTGTTGCCA+TGG 0.428743 2.4:+32492965 MS.gene77117:CDS
TCCTCCGGCGGCATTTCTCA+GGG 0.436877 2.4:+32493320 MS.gene77117:CDS
CGGACAGGGGCTGTTCTACA+TGG 0.447788 2.4:+32492494 MS.gene77117:CDS
TCGGGATGAGGAGGAGAATT+GGG 0.451241 2.4:-32492765 None:intergenic
GACGATGATTCCATCGATCT+TGG 0.451452 2.4:+32492663 MS.gene77117:CDS
GATTTGTTGCACGAGTGTTT+GGG 0.452697 2.4:+32493211 MS.gene77117:CDS
AGAGATTTGTTACGGAGAAC+CGG 0.452877 2.4:+32493352 MS.gene77117:CDS
GTGGGAATGTGGCTAATAAT+GGG 0.456886 2.4:+32493015 MS.gene77117:CDS
CCAGTATCTCGGCAGCAATC+AGG 0.457630 2.4:-32492584 None:intergenic
GAGCACTTCCACCAGCCGTT+AGG 0.466631 2.4:+32492447 MS.gene77117:CDS
CGAGATACTGGATCCCTTGG+AGG 0.468114 2.4:+32492596 MS.gene77117:CDS
ATCGTCGTCGTTGTCTTCAT+CGG 0.472155 2.4:-32492646 None:intergenic
ACATCAACATCCGCTGGATC+CGG 0.472816 2.4:-32492738 None:intergenic
TTGATTGGCATCATGAACTT+CGG 0.473733 2.4:-32492784 None:intergenic
ATGAACTACGACACAGAGTC+TGG 0.481562 2.4:+32492337 MS.gene77117:CDS
ACAGGAGTTGAAGGAGGTGC+AGG 0.491094 2.4:-32492708 None:intergenic
CATGGTGAAGACTAGCAATC+TGG 0.491937 2.4:+32493073 MS.gene77117:CDS
ACGGAGAACCGGCTGGCAGT+TGG 0.492128 2.4:+32493363 MS.gene77117:CDS
GCTGGATCCGGTTCTGCCAC+AGG 0.493035 2.4:-32492726 None:intergenic
GATGCTGCTGCTGTTGCCAT+GGG 0.497052 2.4:+32492966 MS.gene77117:CDS
GGACAGAGGCCATCTTCCTC+CGG 0.503311 2.4:+32493305 MS.gene77117:CDS
CGGCGGCATTTCTCAGGGGC+GGG 0.503464 2.4:+32493325 MS.gene77117:CDS
TGGGCTTTGACTTTCCGTGC+AGG 0.505024 2.4:+32493271 MS.gene77117:CDS
CCCTAGCATACATAACATAT+GGG 0.511261 2.4:-32493419 None:intergenic
GCATCATGAACTTCGGGATG+AGG 0.512150 2.4:-32492777 None:intergenic
GGTTCTGCCACAGGAGTTGA+AGG 0.512252 2.4:-32492717 None:intergenic
GCAAAATCAAAGAAATTATG+TGG 0.521917 2.4:-32492843 None:intergenic
TGCCGAGATACTGGATCCCT+TGG 0.522650 2.4:+32492593 MS.gene77117:CDS
TCATACTTCAACAACCACTT+TGG 0.527976 2.4:+32492312 MS.gene77117:CDS
ACCCATATGTTATGTATGCT+AGG 0.529159 2.4:+32493418 MS.gene77117:CDS
CGGCATTTCTCAGGGGCGGG+TGG 0.532162 2.4:+32493328 MS.gene77117:CDS
GTATGCTAGGGAAGCTCGGA+TGG 0.537149 2.4:+32493431 MS.gene77117:CDS
AAGTGGTTGTTGAAGTATGA+TGG 0.538484 2.4:-32492309 None:intergenic
AACATCAATGCCAAGATCGA+TGG 0.539611 2.4:-32492673 None:intergenic
GCGTCTAAGTTACAACGGAC+AGG 0.542298 2.4:+32492479 MS.gene77117:CDS
CATCCTACTTACAGATGCCA+TGG 0.546268 2.4:+32493251 MS.gene77117:CDS
ATTTGTTACGGAGAACCGGC+TGG 0.553165 2.4:+32493356 MS.gene77117:CDS
CCGGCGGCATTTCTCAGGGG+CGG 0.554229 2.4:+32493324 MS.gene77117:CDS
TCAAAGAAATTATGTGGAGG+CGG 0.554584 2.4:-32492837 None:intergenic
ATCTTCATCGTTGATATCCA+TGG 0.554841 2.4:-32492871 None:intergenic
CCCATATGTTATGTATGCTA+GGG 0.556719 2.4:+32493419 MS.gene77117:CDS
ACTCGACATGACCGAGCTAA+CGG 0.557804 2.4:+32492527 MS.gene77117:CDS
CTCCAAGGGATCCAGTATCT+CGG 0.558445 2.4:-32492595 None:intergenic
TAGAAGACACAAGAGGTTCG+AGG 0.561323 2.4:+32492361 MS.gene77117:CDS
TGTGGCTAATAATGGGAGAG+TGG 0.562385 2.4:+32493022 MS.gene77117:CDS
TCATAGGAAGTGATGGGACG+CGG 0.562900 2.4:+32492394 MS.gene77117:CDS
TCCTTCCACTTGATCCTGCA+CGG 0.565208 2.4:-32493285 None:intergenic
TGCACCTCCTTCAACTCCTG+TGG 0.569911 2.4:+32492710 MS.gene77117:CDS
GGTGCAACATCAACATCCGC+TGG 0.570592 2.4:-32492744 None:intergenic
GTATAATTCATACTCCTCCA+AGG 0.570762 2.4:-32492610 None:intergenic
TTATGTATGCTAGGGAAGCT+CGG 0.572454 2.4:+32493427 MS.gene77117:CDS
GGCATCTGTAAGTAGGATGG+TGG 0.575659 2.4:-32493247 None:intergenic
CGTCTAAGTTACAACGGACA+GGG 0.576116 2.4:+32492480 MS.gene77117:CDS
GGTGAAAACATGGGTGCAGG+TGG 0.578164 2.4:+32492996 MS.gene77117:CDS
ACTTTCCGTGCAGGATCAAG+TGG 0.580280 2.4:+32493280 MS.gene77117:CDS
GAAATGCCGCCGGAGGAAGA+TGG 0.581201 2.4:-32493314 None:intergenic
CATGGGTGCAGGTGGGAATG+TGG 0.582106 2.4:+32493004 MS.gene77117:CDS
CCTCTCACGAACCGTTAGCT+CGG 0.586517 2.4:-32492538 None:intergenic
CGGGTGGAAGAGATTTGTTA+CGG 0.590170 2.4:+32493344 MS.gene77117:CDS
CCGAGCTAACGGTTCGTGAG+AGG 0.592918 2.4:+32492538 MS.gene77117:CDS
GGAGTTGAAGGAGGTGCAGG+AGG 0.594963 2.4:-32492705 None:intergenic
GGGTAACAATGGTGAAAACA+TGG 0.597431 2.4:+32492986 MS.gene77117:CDS
TGTGGCAGAACCGGATCCAG+CGG 0.598738 2.4:+32492728 MS.gene77117:CDS
TCATGAACTTCGGGATGAGG+AGG 0.603718 2.4:-32492774 None:intergenic
CCCCTGAGAAATGCCGCCGG+AGG 0.604022 2.4:-32493321 None:intergenic
CATGGCATCTGTAAGTAGGA+TGG 0.604155 2.4:-32493250 None:intergenic
GTGCAACAAATCTTCTTGGT+AGG 0.604465 2.4:-32493200 None:intergenic
GCAGTATGAGACCTAACGGC+TGG 0.608111 2.4:-32492458 None:intergenic
AGCCCATGGCATCTGTAAGT+AGG 0.608677 2.4:-32493254 None:intergenic
GCTGGTAACAACAATGGTGA+TGG 0.608883 2.4:+32492915 MS.gene77117:CDS
CACAACAATCGATCCAGACA+AGG 0.610416 2.4:+32491586 MS.gene77117:CDS
ATCTCGGCAGCAATCAGGAA+AGG 0.610893 2.4:-32492579 None:intergenic
ATCCTACTTACAGATGCCAT+GGG 0.611432 2.4:+32493252 MS.gene77117:CDS
TGTCAGACTTGCAGAATACA+TGG 0.612787 2.4:+32493055 MS.gene77117:CDS
CAGAGGCCATCTTCCTCCGG+CGG 0.613392 2.4:+32493308 MS.gene77117:CDS
GGATATCAACGATGAAGATG+TGG 0.616665 2.4:+32492875 MS.gene77117:CDS
TATAATTCATACTCCTCCAA+GGG 0.616989 2.4:-32492609 None:intergenic
GTATGAGACCTAACGGCTGG+TGG 0.617542 2.4:-32492455 None:intergenic
AATGGTGAAAACATGGGTGC+AGG 0.619307 2.4:+32492993 MS.gene77117:CDS
CCTCCGGCGGCATTTCTCAG+GGG 0.623483 2.4:+32493321 MS.gene77117:CDS
CCGCCCCTGAGAAATGCCGC+CGG 0.627703 2.4:-32493324 None:intergenic
GCATCTGTAAGTAGGATGGT+GGG 0.632206 2.4:-32493246 None:intergenic
GCACGGAAAGTCAAAGCCCA+TGG 0.634938 2.4:-32493268 None:intergenic
TCCGTGCAGGATCAAGTGGA+AGG 0.637315 2.4:+32493284 MS.gene77117:CDS
TGGGCATCATCGGCATTGTG+TGG 0.641687 2.4:-32492810 None:intergenic
GTCGTAGTTCATCACCAAAG+TGG 0.648562 2.4:-32492326 None:intergenic
AGACGCAGTATGAGACCTAA+CGG 0.653534 2.4:-32492462 None:intergenic
TCGGCAGCAATCAGGAAAGG+AGG 0.654636 2.4:-32492576 None:intergenic
GCTGTTGCCATGGGTAACAA+TGG 0.655928 2.4:+32492975 MS.gene77117:CDS
GCAGCATCAAAGAAGTTATG+CGG 0.656305 2.4:-32492951 None:intergenic
GAAGGTATAGACAATGCTGC+TGG 0.657055 2.4:+32492897 MS.gene77117:CDS
GGACAGGGGCTGTTCTACAT+GGG 0.657468 2.4:+32492495 MS.gene77117:CDS
GAACTTCCTGAATGTAAGCG+CGG 0.670661 2.4:-32491561 None:intergenic
GTGAAAACATGGGTGCAGGT+GGG 0.671071 2.4:+32492997 MS.gene77117:CDS
GTTGTCTTCATCGGATGACG+AGG 0.675992 2.4:-32492637 None:intergenic
GCATCAAAGAAGTTATGCGG+AGG 0.686757 2.4:-32492948 None:intergenic
AGGATCAAGTGGAAGGACAG+AGG 0.686964 2.4:+32493291 MS.gene77117:CDS
GTCTGGTTAGAAGACACAAG+AGG 0.687596 2.4:+32492354 MS.gene77117:CDS
ATCAACGATGAAGATGTGGA+AGG 0.687890 2.4:+32492879 MS.gene77117:CDS
ATACTGCGTCTAAGTTACAA+CGG 0.691908 2.4:+32492474 MS.gene77117:CDS
AAATCAAAGAAATTATGTGG+AGG 0.696121 2.4:-32492840 None:intergenic
TCTGCCACAGGAGTTGAAGG+AGG 0.696157 2.4:-32492714 None:intergenic
TCTGTAAGTAGGATGGTGGG+CGG 0.704271 2.4:-32493243 None:intergenic
GTCTAAGTTACAACGGACAG+GGG 0.730281 2.4:+32492481 MS.gene77117:CDS
GGTAACAATGGTGAAAACAT+GGG 0.742892 2.4:+32492987 MS.gene77117:CDS
AATGCTGCTGGTAACAACAA+TGG 0.748660 2.4:+32492909 MS.gene77117:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTTAAAAAAGAACAC+AGG + chr2.4:32492192-32492211 MS.gene77117:intron 15.0%
!!! AAATGATAACAATTTAAAAC+AGG - chr2.4:32493170-32493189 None:intergenic 15.0%
!!! AATTACTGTTATATAGTTTT+TGG + chr2.4:32493129-32493148 MS.gene77117:intron 15.0%
! AAATCAAAGAAATTATGTGG+AGG - chr2.4:32492843-32492862 None:intergenic 25.0%
! AGTACACAAAATCTTAGATA+CGG - chr2.4:32491852-32491871 None:intergenic 25.0%
! GCAAAATCAAAGAAATTATG+TGG - chr2.4:32492846-32492865 None:intergenic 25.0%
!!! CATTTGTTGTTACATGTTTA+AGG + chr2.4:32491739-32491758 MS.gene77117:intron 25.0%
AAATATATTGCAGAGTGACA+AGG - chr2.4:32491935-32491954 None:intergenic 30.0%
AGTACACATTATCGTAGATA+CGG - chr2.4:32492039-32492058 None:intergenic 30.0%
TATAATTCATACTCCTCCAA+GGG - chr2.4:32492612-32492631 None:intergenic 30.0%
TTGTTACTAAACTTTGAGTG+TGG + chr2.4:32491636-32491655 MS.gene77117:intron 30.0%
AAAATCTACCGGATACGAAA+AGG - chr2.4:32491710-32491729 None:intergenic 35.0%
AAATCTACCGGATACGAAAA+GGG - chr2.4:32491709-32491728 None:intergenic 35.0%
AAGTGGTTGTTGAAGTATGA+TGG - chr2.4:32492312-32492331 None:intergenic 35.0%
ACCCATATGTTATGTATGCT+AGG + chr2.4:32493418-32493437 MS.gene77117:CDS 35.0%
ACTCCTTAGCCTATACAAAA+AGG - chr2.4:32492421-32492440 None:intergenic 35.0%
ATACTGCGTCTAAGTTACAA+CGG + chr2.4:32492474-32492493 MS.gene77117:CDS 35.0%
ATCTTCATCGTTGATATCCA+TGG - chr2.4:32492874-32492893 None:intergenic 35.0%
ATTACAGAGTGACAACAAAC+AGG - chr2.4:32491896-32491915 None:intergenic 35.0%
ATTACAGAGTGACAACAAAC+TGG - chr2.4:32491896-32491915 None:intergenic 35.0%
CCCATATGTTATGTATGCTA+GGG + chr2.4:32493419-32493438 MS.gene77117:CDS 35.0%
CCCTAGCATACATAACATAT+GGG - chr2.4:32493422-32493441 None:intergenic 35.0%
GGTAACAATGGTGAAAACAT+GGG + chr2.4:32492987-32493006 MS.gene77117:CDS 35.0%
GTATAATTCATACTCCTCCA+AGG - chr2.4:32492613-32492632 None:intergenic 35.0%
TCAAAGAAATTATGTGGAGG+CGG - chr2.4:32492840-32492859 None:intergenic 35.0%
TCATACTTCAACAACCACTT+TGG + chr2.4:32492312-32492331 MS.gene77117:CDS 35.0%
TCCCTAGCATACATAACATA+TGG - chr2.4:32493423-32493442 None:intergenic 35.0%
TTACAGAGTGACAACAAACT+GGG - chr2.4:32492082-32492101 None:intergenic 35.0%
TTCTTGTGTGTGCATAATAG+TGG - chr2.4:32491828-32491847 None:intergenic 35.0%
TTGATTGGCATCATGAACTT+CGG - chr2.4:32492787-32492806 None:intergenic 35.0%
TTTACAGAGTGACATCAAAC+TGG - chr2.4:32492121-32492140 None:intergenic 35.0%
! GAAGTTGTCACTTGTAATGT+AGG + chr2.4:32492272-32492291 MS.gene77117:intron 35.0%
! GTGTTTTCATAGGAAGTGAT+GGG + chr2.4:32492388-32492407 MS.gene77117:CDS 35.0%
AACATCAATGCCAAGATCGA+TGG - chr2.4:32492676-32492695 None:intergenic 40.0%
AACCAACAAGAAGGCAAACA+TGG - chr2.4:32491772-32491791 None:intergenic 40.0%
AATGCTGCTGGTAACAACAA+TGG + chr2.4:32492909-32492928 MS.gene77117:CDS 40.0%
ACTCGTGCAACAAATCTTCT+TGG - chr2.4:32493207-32493226 None:intergenic 40.0%
ATCAACGATGAAGATGTGGA+AGG + chr2.4:32492879-32492898 MS.gene77117:CDS 40.0%
ATCCTACTTACAGATGCCAT+GGG + chr2.4:32493252-32493271 MS.gene77117:CDS 40.0%
ATGTTTGCCTTCTTGTTGGT+TGG + chr2.4:32491771-32491790 MS.gene77117:intron 40.0%
GACTATGTTTGCCTTCTTGT+TGG + chr2.4:32491767-32491786 MS.gene77117:intron 40.0%
GCAGCATCAAAGAAGTTATG+CGG - chr2.4:32492954-32492973 None:intergenic 40.0%
GGATATCAACGATGAAGATG+TGG + chr2.4:32492875-32492894 MS.gene77117:CDS 40.0%
GGGTAACAATGGTGAAAACA+TGG + chr2.4:32492986-32493005 MS.gene77117:CDS 40.0%
GTCACTTGTAATGTAGGATG+AGG + chr2.4:32492278-32492297 MS.gene77117:intron 40.0%
GTGCAACAAATCTTCTTGGT+AGG - chr2.4:32493203-32493222 None:intergenic 40.0%
GTGGGAATGTGGCTAATAAT+GGG + chr2.4:32493015-32493034 MS.gene77117:CDS 40.0%
TAAGACACACTTACCTTGTC+TGG - chr2.4:32491602-32491621 None:intergenic 40.0%
TCATCATCCAACCAACAAGA+AGG - chr2.4:32491781-32491800 None:intergenic 40.0%
TGAAGCCATGCAAAATCTAC+CGG - chr2.4:32491721-32491740 None:intergenic 40.0%
TGATTGGCATCATGAACTTC+GGG - chr2.4:32492786-32492805 None:intergenic 40.0%
TGTCAGACTTGCAGAATACA+TGG + chr2.4:32493055-32493074 MS.gene77117:CDS 40.0%
TTATGTATGCTAGGGAAGCT+CGG + chr2.4:32493427-32493446 MS.gene77117:CDS 40.0%
! GGACCTTTTTGTATAGGCTA+AGG + chr2.4:32492415-32492434 MS.gene77117:CDS 40.0%
! GGTGTTTTCATAGGAAGTGA+TGG + chr2.4:32492387-32492406 MS.gene77117:CDS 40.0%
! GTTTTCACCATTGTTACCCA+TGG - chr2.4:32492985-32493004 None:intergenic 40.0%
!! AATTATGTGGAGGCGGATTT+TGG - chr2.4:32492833-32492852 None:intergenic 40.0%
!! AGAGATTTGTTACGGAGAAC+CGG + chr2.4:32493352-32493371 MS.gene77117:CDS 40.0%
!! AGATTTGTTGCACGAGTGTT+TGG + chr2.4:32493210-32493229 MS.gene77117:CDS 40.0%
!! ATTATGTGGAGGCGGATTTT+GGG - chr2.4:32492832-32492851 None:intergenic 40.0%
!! GATTTGTTGCACGAGTGTTT+GGG + chr2.4:32493211-32493230 MS.gene77117:CDS 40.0%
!!! TCGAGGTTTGGTGTTTTCAT+AGG + chr2.4:32492378-32492397 MS.gene77117:CDS 40.0%
AATGGTGAAAACATGGGTGC+AGG + chr2.4:32492993-32493012 MS.gene77117:CDS 45.0%
AGACGCAGTATGAGACCTAA+CGG - chr2.4:32492465-32492484 None:intergenic 45.0%
ATCGTCGTCGTTGTCTTCAT+CGG - chr2.4:32492649-32492668 None:intergenic 45.0%
ATGAACTACGACACAGAGTC+TGG + chr2.4:32492337-32492356 MS.gene77117:CDS 45.0%
CACAACAATCGATCCAGACA+AGG + chr2.4:32491586-32491605 MS.gene77117:CDS 45.0%
CATCCTACTTACAGATGCCA+TGG + chr2.4:32493251-32493270 MS.gene77117:CDS 45.0%
CATGGCATCTGTAAGTAGGA+TGG - chr2.4:32493253-32493272 None:intergenic 45.0%
CATGGTGAAGACTAGCAATC+TGG + chr2.4:32493073-32493092 MS.gene77117:CDS 45.0%
CGTCTAAGTTACAACGGACA+GGG + chr2.4:32492480-32492499 MS.gene77117:CDS 45.0%
GAACTTCCTGAATGTAAGCG+CGG - chr2.4:32491564-32491583 None:intergenic 45.0%
GACCATGTTTGCCTTCTTGT+TGG + chr2.4:32491956-32491975 MS.gene77117:intron 45.0%
GACGATGATTCCATCGATCT+TGG + chr2.4:32492663-32492682 MS.gene77117:CDS 45.0%
GCATCAAAGAAGTTATGCGG+AGG - chr2.4:32492951-32492970 None:intergenic 45.0%
GCATCTGTAAGTAGGATGGT+GGG - chr2.4:32493249-32493268 None:intergenic 45.0%
GCTGGTAACAACAATGGTGA+TGG + chr2.4:32492915-32492934 MS.gene77117:CDS 45.0%
GGCATTGTGTGGTTGTTGAT+TGG - chr2.4:32492802-32492821 None:intergenic 45.0%
GGTGGGAATGTGGCTAATAA+TGG + chr2.4:32493014-32493033 MS.gene77117:CDS 45.0%
GTATAGGCTAAGGAGTTTGC+TGG + chr2.4:32492425-32492444 MS.gene77117:CDS 45.0%
GTCGTAGTTCATCACCAAAG+TGG - chr2.4:32492329-32492348 None:intergenic 45.0%
GTCTAAGTTACAACGGACAG+GGG + chr2.4:32492481-32492500 MS.gene77117:CDS 45.0%
GTCTGGTTAGAAGACACAAG+AGG + chr2.4:32492354-32492373 MS.gene77117:CDS 45.0%
GTGAAGACTAGCAATCTGGT+AGG + chr2.4:32493077-32493096 MS.gene77117:intron 45.0%
TAGAAGACACAAGAGGTTCG+AGG + chr2.4:32492361-32492380 MS.gene77117:CDS 45.0%
! CGTATCCGGTAGATTTTGCA+TGG + chr2.4:32491713-32491732 MS.gene77117:intron 45.0%
! GAAGGTATAGACAATGCTGC+TGG + chr2.4:32492897-32492916 MS.gene77117:CDS 45.0%
! TGTGGCTAATAATGGGAGAG+TGG + chr2.4:32493022-32493041 MS.gene77117:CDS 45.0%
!! CGGGTGGAAGAGATTTGTTA+CGG + chr2.4:32493344-32493363 MS.gene77117:CDS 45.0%
!! CTTTGATTTTGCTGCTGCCA+TGG + chr2.4:32492854-32492873 MS.gene77117:CDS 45.0%
!! TGGCAGTTGGGAAAAGTGTT+AGG + chr2.4:32493376-32493395 MS.gene77117:CDS 45.0%
ACATCAACATCCGCTGGATC+CGG - chr2.4:32492741-32492760 None:intergenic 50.0%
ACTCGACATGACCGAGCTAA+CGG + chr2.4:32492527-32492546 MS.gene77117:CDS 50.0%
ACTTTCCGTGCAGGATCAAG+TGG + chr2.4:32493280-32493299 MS.gene77117:CDS 50.0%
AGCCCATGGCATCTGTAAGT+AGG - chr2.4:32493257-32493276 None:intergenic 50.0%
AGGATCAAGTGGAAGGACAG+AGG + chr2.4:32493291-32493310 MS.gene77117:CDS 50.0%
ATCTCGGCAGCAATCAGGAA+AGG - chr2.4:32492582-32492601 None:intergenic 50.0%
CTCCAAGGGATCCAGTATCT+CGG - chr2.4:32492598-32492617 None:intergenic 50.0%
GACACAAGAGGTTCGAGGTT+TGG + chr2.4:32492366-32492385 MS.gene77117:CDS 50.0%
GCATCATGAACTTCGGGATG+AGG - chr2.4:32492780-32492799 None:intergenic 50.0%
GCGTCTAAGTTACAACGGAC+AGG + chr2.4:32492479-32492498 MS.gene77117:CDS 50.0%
GCTGTTGCCATGGGTAACAA+TGG + chr2.4:32492975-32492994 MS.gene77117:CDS 50.0%
GGCATCTGTAAGTAGGATGG+TGG - chr2.4:32493250-32493269 None:intergenic 50.0%
GTGAAAACATGGGTGCAGGT+GGG + chr2.4:32492997-32493016 MS.gene77117:CDS 50.0%
GTTGTCTTCATCGGATGACG+AGG - chr2.4:32492640-32492659 None:intergenic 50.0%
TCATGAACTTCGGGATGAGG+AGG - chr2.4:32492777-32492796 None:intergenic 50.0%
TCCTTCCACTTGATCCTGCA+CGG - chr2.4:32493288-32493307 None:intergenic 50.0%
TCGGGATGAGGAGGAGAATT+GGG - chr2.4:32492768-32492787 None:intergenic 50.0%
TTCAACTCCTGTGGCAGAAC+CGG + chr2.4:32492719-32492738 MS.gene77117:CDS 50.0%
TTCGGGATGAGGAGGAGAAT+TGG - chr2.4:32492769-32492788 None:intergenic 50.0%
! ATTTGTTACGGAGAACCGGC+TGG + chr2.4:32493356-32493375 MS.gene77117:CDS 50.0%
! CCTGTCTCCCTTTTCGTATC+CGG + chr2.4:32491699-32491718 MS.gene77117:intron 50.0%
! GGACGCGGACCTTTTTGTAT+AGG + chr2.4:32492409-32492428 MS.gene77117:CDS 50.0%
! TCATAGGAAGTGATGGGACG+CGG + chr2.4:32492394-32492413 MS.gene77117:CDS 50.0%
!! AGGCGGATTTTGGGCATCAT+CGG - chr2.4:32492823-32492842 None:intergenic 50.0%
!! TCTGTAAGTAGGATGGTGGG+CGG - chr2.4:32493246-32493265 None:intergenic 50.0%
CCAGTATCTCGGCAGCAATC+AGG - chr2.4:32492587-32492606 None:intergenic 55.0%
CCGGATACGAAAAGGGAGAC+AGG - chr2.4:32491702-32491721 None:intergenic 55.0%
CCTCTCACGAACCGTTAGCT+CGG - chr2.4:32492541-32492560 None:intergenic 55.0%
CCTGATTGCTGCCGAGATAC+TGG + chr2.4:32492584-32492603 MS.gene77117:CDS 55.0%
CGAGATACTGGATCCCTTGG+AGG + chr2.4:32492596-32492615 MS.gene77117:CDS 55.0%
GATGCTGCTGCTGTTGCCAT+GGG + chr2.4:32492966-32492985 MS.gene77117:CDS 55.0%
GCACGGAAAGTCAAAGCCCA+TGG - chr2.4:32493271-32493290 None:intergenic 55.0%
GCAGTATGAGACCTAACGGC+TGG - chr2.4:32492461-32492480 None:intergenic 55.0%
GGACAGGGGCTGTTCTACAT+GGG + chr2.4:32492495-32492514 MS.gene77117:CDS 55.0%
GGTGAAAACATGGGTGCAGG+TGG + chr2.4:32492996-32493015 MS.gene77117:CDS 55.0%
GGTGCAACATCAACATCCGC+TGG - chr2.4:32492747-32492766 None:intergenic 55.0%
GGTTCTGCCACAGGAGTTGA+AGG - chr2.4:32492720-32492739 None:intergenic 55.0%
GTATGAGACCTAACGGCTGG+TGG - chr2.4:32492458-32492477 None:intergenic 55.0%
GTATGCTAGGGAAGCTCGGA+TGG + chr2.4:32493431-32493450 MS.gene77117:CDS 55.0%
TCCGTGCAGGATCAAGTGGA+AGG + chr2.4:32493284-32493303 MS.gene77117:CDS 55.0%
TCGGCAGCAATCAGGAAAGG+AGG - chr2.4:32492579-32492598 None:intergenic 55.0%
TCTGCCACAGGAGTTGAAGG+AGG - chr2.4:32492717-32492736 None:intergenic 55.0%
TGATGCTGCTGCTGTTGCCA+TGG + chr2.4:32492965-32492984 MS.gene77117:CDS 55.0%
TGCACCTCCTTCAACTCCTG+TGG + chr2.4:32492710-32492729 MS.gene77117:CDS 55.0%
TGCCGAGATACTGGATCCCT+TGG + chr2.4:32492593-32492612 MS.gene77117:CDS 55.0%
TGGGCATCATCGGCATTGTG+TGG - chr2.4:32492813-32492832 None:intergenic 55.0%
! ACAGGAGTTGAAGGAGGTGC+AGG - chr2.4:32492711-32492730 None:intergenic 55.0%
! CACTTTTCCCAACTGCCAGC+CGG - chr2.4:32493374-32493393 None:intergenic 55.0%
! TGGGCTTTGACTTTCCGTGC+AGG + chr2.4:32493271-32493290 MS.gene77117:CDS 55.0%
CATGGGTGCAGGTGGGAATG+TGG + chr2.4:32493004-32493023 MS.gene77117:CDS 60.0%
CCGAGCTAACGGTTCGTGAG+AGG + chr2.4:32492538-32492557 MS.gene77117:CDS 60.0%
CGGACAGGGGCTGTTCTACA+TGG + chr2.4:32492494-32492513 MS.gene77117:CDS 60.0%
GAAATGCCGCCGGAGGAAGA+TGG - chr2.4:32493317-32493336 None:intergenic 60.0%
GAGCACTTCCACCAGCCGTT+AGG + chr2.4:32492447-32492466 MS.gene77117:CDS 60.0%
GGACAGAGGCCATCTTCCTC+CGG + chr2.4:32493305-32493324 MS.gene77117:CDS 60.0%
TCCTCCGGCGGCATTTCTCA+GGG + chr2.4:32493320-32493339 MS.gene77117:CDS 60.0%
TGTGGCAGAACCGGATCCAG+CGG + chr2.4:32492728-32492747 MS.gene77117:CDS 60.0%
TTCCTCCGGCGGCATTTCTC+AGG + chr2.4:32493319-32493338 MS.gene77117:CDS 60.0%
! GGAGTTGAAGGAGGTGCAGG+AGG - chr2.4:32492708-32492727 None:intergenic 60.0%
ACGGAGAACCGGCTGGCAGT+TGG + chr2.4:32493363-32493382 MS.gene77117:CDS 65.0%
CAGAGGCCATCTTCCTCCGG+CGG + chr2.4:32493308-32493327 MS.gene77117:CDS 65.0%
CCTCCGGCGGCATTTCTCAG+GGG + chr2.4:32493321-32493340 MS.gene77117:CDS 65.0%
CGGAGAACCGGCTGGCAGTT+GGG + chr2.4:32493364-32493383 MS.gene77117:CDS 65.0%
GCTGGATCCGGTTCTGCCAC+AGG - chr2.4:32492729-32492748 None:intergenic 65.0%
CCCCTGAGAAATGCCGCCGG+AGG - chr2.4:32493324-32493343 None:intergenic 70.0%
CCGCCCCTGAGAAATGCCGC+CGG - chr2.4:32493327-32493346 None:intergenic 70.0%
CCGGCGGCATTTCTCAGGGG+CGG + chr2.4:32493324-32493343 MS.gene77117:CDS 70.0%
CGGCATTTCTCAGGGGCGGG+TGG + chr2.4:32493328-32493347 MS.gene77117:CDS 70.0%
CGGCGGCATTTCTCAGGGGC+GGG + chr2.4:32493325-32493344 MS.gene77117:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32491557 32493461 32491557 ID=MS.gene77117
chr2.4 mRNA 32491557 32493461 32491557 ID=MS.gene77117.t1;Parent=MS.gene77117
chr2.4 exon 32491557 32491607 32491557 ID=MS.gene77117.t1.exon1;Parent=MS.gene77117.t1
chr2.4 CDS 32491557 32491607 32491557 ID=cds.MS.gene77117.t1;Parent=MS.gene77117.t1
chr2.4 exon 32492294 32493094 32492294 ID=MS.gene77117.t1.exon2;Parent=MS.gene77117.t1
chr2.4 CDS 32492294 32493094 32492294 ID=cds.MS.gene77117.t1;Parent=MS.gene77117.t1
chr2.4 exon 32493198 32493461 32493198 ID=MS.gene77117.t1.exon3;Parent=MS.gene77117.t1
chr2.4 CDS 32493198 32493461 32493198 ID=cds.MS.gene77117.t1;Parent=MS.gene77117.t1
Gene Sequence

>MS.gene77117

ATGGCCGCGCTTACATTCAGGAAGTTCTTCACAACAATCGATCCAGACAAGGTAAGTGTGTCTTAATATTCACTAACTTTTGTTACTAAACTTTGAGTGTGGAGTAGATTTTTTAATCAAAAGTACGTTGATTGATTATTTTCCTGTCTCCCTTTTCGTATCCGGTAGATTTTGCATGGCTTCATTTGTTGTTACATGTTTAAGGATTTTGACTATGTTTGCCTTCTTGTTGGTTGGATGATGATGAATCTGTAAGTATAATTTATTTCCACTATTATGCACACACAAGAAACCGTATCTAAGATTTTGTGTACTCTGATTGTTAGATTGATAATTCCTGTTTGTTGTCACTCTGTAATGTTAGTTTGATATTTTCCTTGTCACTCTGCAATATATTTTGACCATGTTTGCCTTCTTGTTGGTTGGATGATGATGAATTGATAACTGTATTTTTTTCAACTATTATGCACACAAAAAAACCGTATCTACGATAATGTGTACTCTGATTGTTACTTTGATATTCCCAGTTTGTTGTCACTCTGTAATGTTAGTTTGAAATTTCCAGTTTGATGTCACTCTGTAAATCACATCGTCGTCAAATGACATCTCAATAAACATCAGTTGTATCTTAAGTAAATTATTTAAAAAAGAACACAGGTTTGTTATTTTCATGAGATATAGTATCTTTCAAATCATTTGTTGTAAAATGACTAATGAAGTTGTCACTTGTAATGTAGGATGAGGCTGATATTCCATCATACTTCAACAACCACTTTGGTGATGAACTACGACACAGAGTCTGGTTAGAAGACACAAGAGGTTCGAGGTTTGGTGTTTTCATAGGAAGTGATGGGACGCGGACCTTTTTGTATAGGCTAAGGAGTTTGCTGGAGCACTTCCACCAGCCGTTAGGTCTCATACTGCGTCTAAGTTACAACGGACAGGGGCTGTTCTACATGGGTGCGCTTACACTCGACATGACCGAGCTAACGGTTCGTGAGAGGCGTACTTATGAACATCCTCCTTTCCTGATTGCTGCCGAGATACTGGATCCCTTGGAGGAGTATGAATTATACAATACCTCGTCATCCGATGAAGACAACGACGACGATGATTCCATCGATCTTGGCATTGATGTTGATGTTCAACCTCCTGCACCTCCTTCAACTCCTGTGGCAGAACCGGATCCAGCGGATGTTGATGTTGCACCCAATTCTCCTCCTCATCCCGAAGTTCATGATGCCAATCAACAACCACACAATGCCGATGATGCCCAAAATCCGCCTCCACATAATTTCTTTGATTTTGCTGCTGCCATGGATATCAACGATGAAGATGTGGAAGGTATAGACAATGCTGCTGGTAACAACAATGGTGATGGAGCACAAAATCCTCCGCATAACTTCTTTGATGCTGCTGCTGTTGCCATGGGTAACAATGGTGAAAACATGGGTGCAGGTGGGAATGTGGCTAATAATGGGAGAGTGGAGAGCTGTGTTGTCAGACTTGCAGAATACATGGTGAAGACTAGCAATCTGGTAGGTGAATTGCATAACTGCTAATGCATAATGCAATTACTGTTATATAGTTTTTGGTAGATATGTCTAACGCCTGTTTTAAATTGTTATCATTTGCATGCAGTACCTACCAAGAAGATTTGTTGCACGAGTGTTTGGGAATGCAACACCGCCCACCATCCTACTTACAGATGCCATGGGCTTTGACTTTCCGTGCAGGATCAAGTGGAAGGACAGAGGCCATCTTCCTCCGGCGGCATTTCTCAGGGGCGGGTGGAAGAGATTTGTTACGGAGAACCGGCTGGCAGTTGGGAAAAGTGTTAGGTTGTCTGTCAATTTAGACAACCCATATGTTATGTATGCTAGGGAAGCTCGGATGGAGATTTGA
Protein sequence

>MS.gene77117.t1

MAALTFRKFFTTIDPDKDEADIPSYFNNHFGDELRHRVWLEDTRGSRFGVFIGSDGTRTFLYRLRSLLEHFHQPLGLILRLSYNGQGLFYMGALTLDMTELTVRERRTYEHPPFLIAAEILDPLEEYELYNTSSSDEDNDDDDSIDLGIDVDVQPPAPPSTPVAEPDPADVDVAPNSPPHPEVHDANQQPHNADDAQNPPPHNFFDFAAAMDINDEDVEGIDNAAGNNNGDGAQNPPHNFFDAAAVAMGNNGENMGAGGNVANNGRVESCVVRLAEYMVKTSNLYLPRRFVARVFGNATPPTILLTDAMGFDFPCRIKWKDRGHLPPAAFLRGGWKRFVTENRLAVGKSVRLSVNLDNPYVMYAREARMEI