AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77146

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77146.t1 MTR_2g061030 90.332 331 15 4 1 317 1 328 0.0 533
MS.gene77146.t1 MTR_7g093430 71.204 191 33 4 114 302 103 273 1.76e-82 252
MS.gene77146.t1 MTR_1g054285 51.622 339 81 12 1 315 1 280 5.05e-82 251
MS.gene77146.t1 MTR_4g100550 55.777 251 72 6 75 313 22 245 8.08e-73 226
MS.gene77146.t1 MTR_5g013010 61.972 213 46 8 111 312 72 260 3.74e-70 220
MS.gene77146.t1 MTR_4g097600 76.119 134 29 1 168 301 109 239 4.39e-67 211
MS.gene77146.t1 MTR_5g014890 63.462 156 50 2 175 327 113 264 1.57e-61 197
MS.gene77146.t1 MTR_8g006705 57.277 213 65 4 102 300 58 258 4.80e-61 197
MS.gene77146.t1 MTR_1g017090 63.433 134 42 2 171 302 117 245 5.99e-53 176
MS.gene77146.t1 MTR_4g084750 66.129 124 35 2 184 300 59 182 4.41e-49 163
MS.gene77146.t1 MTR_3g103590 62.676 142 49 2 175 316 134 271 1.11e-44 155
MS.gene77146.t1 MTR_2g038625 54.545 132 57 2 189 318 82 212 4.55e-38 135
MS.gene77146.t1 MTR_2g038580 54.545 132 57 2 189 318 82 212 4.55e-38 135
MS.gene77146.t1 MTR_4g126900 55.462 119 39 2 191 300 90 203 9.21e-34 124
MS.gene77146.t1 MTR_5g013010 55.000 140 29 7 111 240 72 187 2.20e-29 112
MS.gene77146.t1 MTR_7g010020 45.946 111 55 3 175 281 67 176 1.79e-18 84.3
MS.gene77146.t1 MTR_6g007647 42.105 114 66 0 189 302 33 146 2.48e-18 83.6
MS.gene77146.t1 MTR_6g007647 42.105 114 66 0 189 302 67 180 2.60e-18 84.0
MS.gene77146.t1 MTR_6g011610 41.085 129 51 2 154 275 79 189 3.19e-18 83.6
MS.gene77146.t1 MTR_4g107650 44.355 124 63 2 159 276 59 182 3.90e-18 84.3
MS.gene77146.t1 MTR_1g061660 44.086 93 52 0 185 277 55 147 4.03e-18 82.4
MS.gene77146.t1 MTR_6g011610 41.085 129 51 2 154 275 60 170 4.33e-18 82.8
MS.gene77146.t1 MTR_5g039000 40.152 132 74 2 145 276 38 164 1.81e-17 81.6
MS.gene77146.t1 MTR_3g080100 43.119 109 55 1 175 276 6 114 2.06e-17 80.1
MS.gene77146.t1 MTR_3g080100 39.200 125 67 2 161 276 29 153 2.65e-17 80.5
MS.gene77146.t1 MTR_7g103340 41.489 94 55 0 183 276 57 150 4.01e-17 79.7
MS.gene77146.t1 MTR_3g086790 37.671 146 79 4 176 310 46 190 4.45e-17 80.9
MS.gene77146.t1 MTR_8g468210 38.732 142 70 3 145 282 46 174 5.39e-17 80.1
MS.gene77146.t1 MTR_5g038280 40.909 132 66 4 153 275 52 180 8.80e-17 80.1
MS.gene77146.t1 MTR_8g089895 46.875 96 44 1 188 276 53 148 4.95e-16 77.8
MS.gene77146.t1 MTR_8g469430 44.681 94 45 1 189 275 89 182 3.15e-15 75.1
MS.gene77146.t1 MTR_5g019650 45.882 85 46 0 189 273 53 137 4.94e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77146.t1 AT5G06710 50.000 326 132 9 1 310 1 311 1.52e-84 258
MS.gene77146.t1 AT4G37790 61.236 178 58 3 151 320 83 257 6.25e-65 206
MS.gene77146.t1 AT2G44910 58.209 201 55 3 113 304 99 279 6.90e-65 207
MS.gene77146.t1 AT5G47370 57.339 218 75 6 111 319 53 261 2.14e-64 205
MS.gene77146.t1 AT3G60390 59.375 192 67 4 113 300 90 274 1.78e-63 204
MS.gene77146.t1 AT4G16780 64.198 162 49 3 163 320 108 264 6.01e-61 196
MS.gene77146.t1 AT4G17460 59.500 200 62 7 113 307 69 254 1.96e-60 195
MS.gene77146.t1 AT2G22800 75.862 116 28 0 186 301 110 225 1.94e-59 192
MS.gene77146.t1 AT2G01430 57.746 142 58 2 175 316 125 264 5.90e-48 162
MS.gene77146.t1 AT1G70920 55.479 146 49 3 173 318 26 155 2.54e-41 142
MS.gene77146.t1 AT1G70920 55.479 146 49 3 173 318 56 185 3.71e-41 142
MS.gene77146.t1 AT5G06710 40.769 260 115 10 1 240 1 241 5.81e-38 136
MS.gene77146.t1 AT2G01430 63.636 66 24 0 175 240 125 190 7.71e-21 89.4
MS.gene77146.t1 AT5G03790 44.681 94 52 0 184 277 72 165 7.77e-18 81.6
MS.gene77146.t1 AT5G06710 35.193 233 112 11 1 213 1 214 2.38e-17 80.5
MS.gene77146.t1 AT5G06710 35.193 233 112 11 1 213 1 214 2.38e-17 80.5
MS.gene77146.t1 AT1G69780 44.554 101 56 0 176 276 72 172 2.98e-17 81.3
MS.gene77146.t1 AT1G26960 36.242 149 72 3 177 321 63 192 9.51e-17 79.0
MS.gene77146.t1 AT1G27045 51.852 81 38 1 175 254 10 90 3.49e-16 75.9
MS.gene77146.t1 AT1G27045 51.852 81 38 1 175 254 10 90 3.49e-16 75.9
MS.gene77146.t1 AT1G27045 51.852 81 38 1 175 254 2 82 4.35e-16 75.5
MS.gene77146.t1 AT1G27045 51.852 81 38 1 175 254 53 133 6.15e-16 76.3
MS.gene77146.t1 AT5G15150 40.909 110 54 2 173 275 103 208 9.06e-16 77.0
MS.gene77146.t1 AT2G36610 40.196 102 53 1 184 277 66 167 9.45e-16 74.7
MS.gene77146.t1 AT1G27045 51.852 81 38 1 175 254 53 133 1.13e-15 75.9
MS.gene77146.t1 AT2G18550 37.671 146 84 2 167 312 40 178 1.62e-15 74.7
MS.gene77146.t1 AT3G01220 42.453 106 55 2 177 278 77 180 2.06e-15 75.9
MS.gene77146.t1 AT3G01470 45.652 92 50 0 189 280 68 159 2.82e-15 75.1
MS.gene77146.t1 AT5G65310 40.909 110 55 2 177 276 57 166 4.57e-15 75.1
MS.gene77146.t1 AT5G65310 40.909 110 55 2 177 276 39 148 5.19e-15 74.7
MS.gene77146.t1 AT5G03790 37.190 121 68 1 184 296 72 192 5.31e-15 72.8
MS.gene77146.t1 AT2G22430 45.455 99 50 1 185 279 58 156 1.09e-14 73.9
MS.gene77146.t1 AT4G40060 39.669 121 67 2 163 279 35 153 3.05e-14 72.4
MS.gene77146.t1 AT4G36740 43.011 93 52 1 183 275 51 142 1.29e-12 66.6
MS.gene77146.t1 AT4G36740 43.011 93 52 1 183 275 50 141 1.31e-12 66.6
MS.gene77146.t1 AT2G46680 40.659 91 54 0 184 274 27 117 3.17e-12 66.2
MS.gene77146.t1 AT2G46680 40.659 91 54 0 184 274 27 117 3.22e-12 66.2

Find 62 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTACCTTGCTCTTCTATTT+TGG 0.134685 2.4:-32175665 None:intergenic
AAAGCCTCTAATTGGATTAA+TGG 0.207556 2.4:+32174316 MS.gene77146:CDS
GACTCTTCAAAGCCTCTAAT+TGG 0.231354 2.4:+32174308 MS.gene77146:CDS
GGTAGGAGTTTGAGTGGTTC+AGG 0.247485 2.4:+32174890 MS.gene77146:CDS
CTTTGTGTAGTACTAGTAAT+TGG 0.274501 2.4:-32174404 None:intergenic
AGTTGATGCAATACAGGATT+AGG 0.292885 2.4:-32174506 None:intergenic
AAGAAGATGCTGCACTATTC+GGG 0.307898 2.4:-32174826 None:intergenic
AATTTATTATCAACCGAGTT+TGG 0.329723 2.4:+32174752 MS.gene77146:CDS
GAAGCCTAAGCCAAGTTCTT+TGG 0.340212 2.4:-32174360 None:intergenic
AAAGAAGATGCTGCACTATT+CGG 0.346517 2.4:-32174827 None:intergenic
TTCAACACCACTTTATCAAT+TGG 0.386708 2.4:+32174380 MS.gene77146:CDS
GAAGAAACTTAGGCTCTCTA+AGG 0.396097 2.4:+32175018 MS.gene77146:CDS
CATCAAGCTTCCAAAGAACT+TGG 0.420365 2.4:+32174350 MS.gene77146:CDS
TGGAGGTTCTTCAAGGGGAT+TGG 0.425919 2.4:+32174772 MS.gene77146:CDS
AGATCAAGTTGATGCAATAC+AGG 0.427277 2.4:-32174512 None:intergenic
CCGAGTTTGGAGGTTCTTCA+AGG 0.430226 2.4:+32174765 MS.gene77146:CDS
CACATAGTTAAAGTAGTTGC+TGG 0.454337 2.4:-32175967 None:intergenic
AAACACTAACAGAAGAGAAT+AGG 0.466193 2.4:+32175878 MS.gene77146:CDS
GCTTCCAAAGAACTTGGCTT+AGG 0.468530 2.4:+32174356 MS.gene77146:CDS
ATGGAGCTAGGTTTAAGCTT+AGG 0.480018 2.4:+32174284 MS.gene77146:CDS
TTCCTCAACTCTCATTCCCA+TGG 0.491801 2.4:+32174537 MS.gene77146:CDS
TTTGGAACCAGACTTCTACT+TGG 0.497117 2.4:-32175647 None:intergenic
AATACTAGTGCCACAATCAA+TGG 0.499863 2.4:+32176042 MS.gene77146:CDS
GCTGGTAATTGCATGTTGAA+TGG 0.500603 2.4:-32175949 None:intergenic
AGTATTAGTGACTGAGGAAG+TGG 0.500829 2.4:-32176025 None:intergenic
GCCCTCGCCAAGTAGAAGTC+TGG 0.501753 2.4:+32175640 MS.gene77146:CDS
CGAGTTTGGAGGTTCTTCAA+GGG 0.504410 2.4:+32174766 MS.gene77146:CDS
GTAACACAAGGAAGAAACTT+AGG 0.517327 2.4:+32175008 MS.gene77146:CDS
TGGAATCCTCCTTCTCAAAA+CGG 0.522286 2.4:+32174557 MS.gene77146:CDS
GAGAAAGAAATCATGGAGCT+AGG 0.525164 2.4:+32174272 None:intergenic
ACAACAAGAAAAGACTCAAA+GGG 0.526599 2.4:+32174451 MS.gene77146:CDS
AATTGGACCAATTGATAAAG+TGG 0.530240 2.4:-32174387 None:intergenic
AACAACAAGAAAAGACTCAA+AGG 0.531710 2.4:+32174450 MS.gene77146:CDS
GATAACTGTGGTGTTGGTAA+TGG 0.532740 2.4:+32174986 MS.gene77146:CDS
GTTGCAACACGTTCACAAGA+AGG 0.536936 2.4:-32175991 None:intergenic
CCTTGAAGAACCTCCAAACT+CGG 0.537882 2.4:-32174765 None:intergenic
TTATTATCAACCGAGTTTGG+AGG 0.547939 2.4:+32174755 MS.gene77146:CDS
AGGAGTTTGAGTGGTTCAGG+AGG 0.550877 2.4:+32174893 MS.gene77146:CDS
TTGCAACACGTTCACAAGAA+GGG 0.553307 2.4:-32175990 None:intergenic
ATGTACAGTCGAAGAGGAAG+TGG 0.554028 2.4:+32174866 MS.gene77146:CDS
AGTGGTGGTAGGAGTTTGAG+TGG 0.555829 2.4:+32174884 MS.gene77146:CDS
CACTAACAGAAGAGAATAGG+AGG 0.565496 2.4:+32175881 MS.gene77146:CDS
GAAGATGATAACTGTGGTGT+TGG 0.570473 2.4:+32174980 MS.gene77146:CDS
ATGGTGAAGGGTATGATCAA+AGG 0.575898 2.4:+32174936 MS.gene77146:CDS
TTTGAGAAGGAGGATTCCAT+GGG 0.580464 2.4:-32174553 None:intergenic
AACCAGACTTCTACTTGGCG+AGG 0.590253 2.4:-32175642 None:intergenic
GAGTTTGGAGGTTCTTCAAG+GGG 0.603616 2.4:+32174767 MS.gene77146:CDS
AGTTCTTTGGAAGCTTGATG+AGG 0.611059 2.4:-32174347 None:intergenic
ATTGGATGTGAACGATGAGA+TGG 0.615034 2.4:+32174790 MS.gene77146:CDS
ATTGTTATTACCATTGATTG+TGG 0.625260 2.4:-32176052 None:intergenic
TTCCATGGGAATGAGAGTTG+AGG 0.625263 2.4:-32174539 None:intergenic
ACCAGACTTCTACTTGGCGA+GGG 0.626726 2.4:-32175641 None:intergenic
CTATGCATGTACAGTCGAAG+AGG 0.633196 2.4:+32174860 MS.gene77146:CDS
TACAGTCGAAGAGGAAGTGG+TGG 0.637044 2.4:+32174869 MS.gene77146:CDS
GTCGAAGAGGAAGTGGTGGT+AGG 0.639483 2.4:+32174873 MS.gene77146:CDS
GGAGTTTGAGTGGTTCAGGA+GGG 0.643496 2.4:+32174894 MS.gene77146:CDS
GGTTCCAAAATAGAAGAGCA+AGG 0.645595 2.4:+32175661 MS.gene77146:CDS
AGAAGATGCTGCACTATTCG+GGG 0.651031 2.4:-32174825 None:intergenic
GGCACTAGTATTAGTGACTG+AGG 0.651032 2.4:-32176031 None:intergenic
AGAGAATAGGAGGCTACACA+AGG 0.664591 2.4:+32175891 MS.gene77146:CDS
AGTGATGAAGATGATAACTG+TGG 0.784782 2.4:+32174974 MS.gene77146:CDS
GTGTTGGTAATGGTAACACA+AGG 0.792389 2.4:+32174996 MS.gene77146:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAATAAAACAAATAAA+TGG - chr2.4:32175534-32175553 None:intergenic 10.0%
!! ACAAAATAAAACAAATAAAT+GGG - chr2.4:32175533-32175552 None:intergenic 10.0%
!!! TTTTTATAGATTTTTTTTTG+TGG - chr2.4:32175215-32175234 None:intergenic 10.0%
!!! TTTTTTGTTTAAAATACTAA+TGG + chr2.4:32175559-32175578 MS.gene77146:intron 10.0%
!! AAGAGTAAAAGATAAAATAT+TGG - chr2.4:32174608-32174627 None:intergenic 15.0%
!! AATATTCTATGTATTGTTAT+TGG + chr2.4:32175316-32175335 MS.gene77146:intron 15.0%
!! AGTGCTTATTATTATTATAA+GGG + chr2.4:32174706-32174725 MS.gene77146:intron 15.0%
!! ATATCTCTAATATATAGTAT+TGG + chr2.4:32175359-32175378 MS.gene77146:intron 15.0%
!! CTATATATTAGAGATATATT+TGG - chr2.4:32175356-32175375 None:intergenic 15.0%
!!! ATAGAATATTTTCTTTTGAA+AGG - chr2.4:32175306-32175325 None:intergenic 15.0%
!!! CAACTTTATTTTTTAAACTT+TGG + chr2.4:32175755-32175774 MS.gene77146:intron 15.0%
!!! TAAAATATTGGTAACTTTTT+AGG - chr2.4:32174596-32174615 None:intergenic 15.0%
!! CAGTGCTTATTATTATTATA+AGG + chr2.4:32174705-32174724 MS.gene77146:intron 20.0%
!! TAATCCTATTTAAAAAACAC+TGG - chr2.4:32174675-32174694 None:intergenic 20.0%
!!! GTTATTATTGTTTTCACAAT+AGG + chr2.4:32174728-32174747 MS.gene77146:intron 20.0%
!!! TTGTTTAAAATACTAATGGT+TGG + chr2.4:32175563-32175582 MS.gene77146:intron 20.0%
! AAAATTATAGTCAAATGCAG+TGG + chr2.4:32175729-32175748 MS.gene77146:intron 25.0%
! AATTTATTATCAACCGAGTT+TGG + chr2.4:32174752-32174771 MS.gene77146:CDS 25.0%
! AGAGAAAAAAAAACACATAC+AGG - chr2.4:32175094-32175113 None:intergenic 25.0%
! ATAAATTTATGTTCTGATGC+AGG + chr2.4:32175803-32175822 MS.gene77146:intron 25.0%
! GAGAAAAAAAAACACATACA+GGG - chr2.4:32175093-32175112 None:intergenic 25.0%
! TGCATAAAAATATGCAAAGT+TGG + chr2.4:32175175-32175194 MS.gene77146:intron 25.0%
!! ATTGTTATTACCATTGATTG+TGG - chr2.4:32176055-32176074 None:intergenic 25.0%
!! ATTTTATGACAATTCTTCCT+TGG + chr2.4:32175506-32175525 MS.gene77146:intron 25.0%
!! TTTTCCATTAATCCAATTAG+AGG - chr2.4:32174323-32174342 None:intergenic 25.0%
!!! GAAACCAGTGTTTTTTAAAT+AGG + chr2.4:32174668-32174687 MS.gene77146:intron 25.0%
!!! TATAGATTTTTTTTTGTGGC+CGG - chr2.4:32175211-32175230 None:intergenic 25.0%
AAAACAAATAAATGGGTCCA+AGG - chr2.4:32175526-32175545 None:intergenic 30.0%
AAACACTAACAGAAGAGAAT+AGG + chr2.4:32175878-32175897 MS.gene77146:CDS 30.0%
AAAGCCTCTAATTGGATTAA+TGG + chr2.4:32174316-32174335 MS.gene77146:CDS 30.0%
AACAACAAGAAAAGACTCAA+AGG + chr2.4:32174450-32174469 MS.gene77146:CDS 30.0%
AATTGGACCAATTGATAAAG+TGG - chr2.4:32174390-32174409 None:intergenic 30.0%
ACAACAAGAAAAGACTCAAA+GGG + chr2.4:32174451-32174470 MS.gene77146:CDS 30.0%
CTTTGTGTAGTACTAGTAAT+TGG - chr2.4:32174407-32174426 None:intergenic 30.0%
TTCAACACCACTTTATCAAT+TGG + chr2.4:32174380-32174399 MS.gene77146:CDS 30.0%
!! ATTGGTAACTTTTTAGGAAG+TGG - chr2.4:32174590-32174609 None:intergenic 30.0%
!! TTGGTAACTTTTTAGGAAGT+GGG - chr2.4:32174589-32174608 None:intergenic 30.0%
!!! ATAGATTTTTTTTTGTGGCC+GGG - chr2.4:32175210-32175229 None:intergenic 30.0%
AAAGAAGATGCTGCACTATT+CGG - chr2.4:32174830-32174849 None:intergenic 35.0%
AATACTAGTGCCACAATCAA+TGG + chr2.4:32176042-32176061 MS.gene77146:CDS 35.0%
AGTGATGAAGATGATAACTG+TGG + chr2.4:32174974-32174993 MS.gene77146:CDS 35.0%
CACATAGTTAAAGTAGTTGC+TGG - chr2.4:32175970-32175989 None:intergenic 35.0%
GTAACACAAGGAAGAAACTT+AGG + chr2.4:32175008-32175027 MS.gene77146:CDS 35.0%
TAAACTGAGTTATGCTCAAG+AGG - chr2.4:32175148-32175167 None:intergenic 35.0%
TATCGACATGTTAGTATGTG+TGG - chr2.4:32175255-32175274 None:intergenic 35.0%
TGTGGTGTAGAAACAAAAAC+TGG + chr2.4:32175593-32175612 MS.gene77146:intron 35.0%
TTATTATCAACCGAGTTTGG+AGG + chr2.4:32174755-32174774 MS.gene77146:CDS 35.0%
! AACAAGAGAGAGTTTTCTGA+TGG + chr2.4:32174917-32174936 MS.gene77146:CDS 35.0%
! AGTTGATGCAATACAGGATT+AGG - chr2.4:32174509-32174528 None:intergenic 35.0%
! TGTACCTTGCTCTTCTATTT+TGG - chr2.4:32175668-32175687 None:intergenic 35.0%
!! AGATCAAGTTGATGCAATAC+AGG - chr2.4:32174515-32174534 None:intergenic 35.0%
!!! TAGATTTTTTTTTGTGGCCG+GGG - chr2.4:32175209-32175228 None:intergenic 35.0%
AAGAAGATGCTGCACTATTC+GGG - chr2.4:32174829-32174848 None:intergenic 40.0%
AGTATTAGTGACTGAGGAAG+TGG - chr2.4:32176028-32176047 None:intergenic 40.0%
ATGGAGCTAGGTTTAAGCTT+AGG + chr2.4:32174284-32174303 MS.gene77146:CDS 40.0%
ATTGGATGTGAACGATGAGA+TGG + chr2.4:32174790-32174809 MS.gene77146:CDS 40.0%
CACTAACAGAAGAGAATAGG+AGG + chr2.4:32175881-32175900 MS.gene77146:CDS 40.0%
CATCAAGCTTCCAAAGAACT+TGG + chr2.4:32174350-32174369 MS.gene77146:CDS 40.0%
CTAATGGTTGGACACAATTG+TGG + chr2.4:32175575-32175594 MS.gene77146:intron 40.0%
GAAGAAACTTAGGCTCTCTA+AGG + chr2.4:32175018-32175037 MS.gene77146:CDS 40.0%
GAAGATGATAACTGTGGTGT+TGG + chr2.4:32174980-32174999 MS.gene77146:CDS 40.0%
GACTCTTCAAAGCCTCTAAT+TGG + chr2.4:32174308-32174327 MS.gene77146:CDS 40.0%
GCTGGTAATTGCATGTTGAA+TGG - chr2.4:32175952-32175971 None:intergenic 40.0%
GGTTCCAAAATAGAAGAGCA+AGG + chr2.4:32175661-32175680 MS.gene77146:CDS 40.0%
TGGAATCCTCCTTCTCAAAA+CGG + chr2.4:32174557-32174576 MS.gene77146:CDS 40.0%
TTGCAACACGTTCACAAGAA+GGG - chr2.4:32175993-32176012 None:intergenic 40.0%
TTTGAGAAGGAGGATTCCAT+GGG - chr2.4:32174556-32174575 None:intergenic 40.0%
! AGTTCTTTGGAAGCTTGATG+AGG - chr2.4:32174350-32174369 None:intergenic 40.0%
! ATGGTGAAGGGTATGATCAA+AGG + chr2.4:32174936-32174955 MS.gene77146:CDS 40.0%
! TGCAGCATCTTCTTTTCAGA+TGG + chr2.4:32174835-32174854 MS.gene77146:CDS 40.0%
! TTTGGAACCAGACTTCTACT+TGG - chr2.4:32175650-32175669 None:intergenic 40.0%
!! AGAGAGTTTTCTGATGGTGA+AGG + chr2.4:32174923-32174942 MS.gene77146:CDS 40.0%
!! GAGAGTTTTCTGATGGTGAA+GGG + chr2.4:32174924-32174943 MS.gene77146:CDS 40.0%
!! GATAACTGTGGTGTTGGTAA+TGG + chr2.4:32174986-32175005 MS.gene77146:CDS 40.0%
!! GTGTTGGTAATGGTAACACA+AGG + chr2.4:32174996-32175015 MS.gene77146:CDS 40.0%
!! TTTTGAGAAGGAGGATTCCA+TGG - chr2.4:32174557-32174576 None:intergenic 40.0%
!!! TTGTTACCGTTTTGAGAAGG+AGG - chr2.4:32174566-32174585 None:intergenic 40.0%
AGAAGATGCTGCACTATTCG+GGG - chr2.4:32174828-32174847 None:intergenic 45.0%
AGAGAATAGGAGGCTACACA+AGG + chr2.4:32175891-32175910 MS.gene77146:CDS 45.0%
ATGTACAGTCGAAGAGGAAG+TGG + chr2.4:32174866-32174885 MS.gene77146:CDS 45.0%
CAAAGTTGGAGTTCAAACCC+CGG + chr2.4:32175189-32175208 MS.gene77146:intron 45.0%
CCTTGAAGAACCTCCAAACT+CGG - chr2.4:32174768-32174787 None:intergenic 45.0%
CGAGTTTGGAGGTTCTTCAA+GGG + chr2.4:32174766-32174785 MS.gene77146:CDS 45.0%
CTATGCATGTACAGTCGAAG+AGG + chr2.4:32174860-32174879 MS.gene77146:CDS 45.0%
GAAGCCTAAGCCAAGTTCTT+TGG - chr2.4:32174363-32174382 None:intergenic 45.0%
GAGTTTGGAGGTTCTTCAAG+GGG + chr2.4:32174767-32174786 MS.gene77146:CDS 45.0%
GCTTCCAAAGAACTTGGCTT+AGG + chr2.4:32174356-32174375 MS.gene77146:CDS 45.0%
GGCACTAGTATTAGTGACTG+AGG - chr2.4:32176034-32176053 None:intergenic 45.0%
GTTGCAACACGTTCACAAGA+AGG - chr2.4:32175994-32176013 None:intergenic 45.0%
TTCCATGGGAATGAGAGTTG+AGG - chr2.4:32174542-32174561 None:intergenic 45.0%
TTCCTCAACTCTCATTCCCA+TGG + chr2.4:32174537-32174556 MS.gene77146:CDS 45.0%
!!! GGGTTGTTACCGTTTTGAGA+AGG - chr2.4:32174569-32174588 None:intergenic 45.0%
AACCAGACTTCTACTTGGCG+AGG - chr2.4:32175645-32175664 None:intergenic 50.0%
ACCAGACTTCTACTTGGCGA+GGG - chr2.4:32175644-32175663 None:intergenic 50.0%
AGGAGTTTGAGTGGTTCAGG+AGG + chr2.4:32174893-32174912 MS.gene77146:CDS 50.0%
AGTGGTGGTAGGAGTTTGAG+TGG + chr2.4:32174884-32174903 MS.gene77146:CDS 50.0%
CCGAGTTTGGAGGTTCTTCA+AGG + chr2.4:32174765-32174784 MS.gene77146:CDS 50.0%
GGAGTTTGAGTGGTTCAGGA+GGG + chr2.4:32174894-32174913 MS.gene77146:CDS 50.0%
GGTAGGAGTTTGAGTGGTTC+AGG + chr2.4:32174890-32174909 MS.gene77146:CDS 50.0%
TACAGTCGAAGAGGAAGTGG+TGG + chr2.4:32174869-32174888 MS.gene77146:CDS 50.0%
TGGAGGTTCTTCAAGGGGAT+TGG + chr2.4:32174772-32174791 MS.gene77146:CDS 50.0%
GTCGAAGAGGAAGTGGTGGT+AGG + chr2.4:32174873-32174892 MS.gene77146:CDS 55.0%
GCCCTCGCCAAGTAGAAGTC+TGG + chr2.4:32175640-32175659 MS.gene77146:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32174284 32176092 32174284 ID=MS.gene77146
chr2.4 mRNA 32174284 32176092 32174284 ID=MS.gene77146.t1;Parent=MS.gene77146
chr2.4 exon 32174284 32174578 32174284 ID=MS.gene77146.t1.exon1;Parent=MS.gene77146.t1
chr2.4 CDS 32174284 32174578 32174284 ID=cds.MS.gene77146.t1;Parent=MS.gene77146.t1
chr2.4 exon 32174750 32175093 32174750 ID=MS.gene77146.t1.exon2;Parent=MS.gene77146.t1
chr2.4 CDS 32174750 32175093 32174750 ID=cds.MS.gene77146.t1;Parent=MS.gene77146.t1
chr2.4 exon 32175603 32175682 32175603 ID=MS.gene77146.t1.exon3;Parent=MS.gene77146.t1
chr2.4 CDS 32175603 32175682 32175603 ID=cds.MS.gene77146.t1;Parent=MS.gene77146.t1
chr2.4 exon 32175825 32176092 32175825 ID=MS.gene77146.t1.exon4;Parent=MS.gene77146.t1
chr2.4 CDS 32175825 32176092 32175825 ID=cds.MS.gene77146.t1;Parent=MS.gene77146.t1
Gene Sequence

>MS.gene77146

ATGGAGCTAGGTTTAAGCTTAGGAGACTCTTCAAAGCCTCTAATTGGATTAATGGAAAAGCATCCTCATCAAGCTTCCAAAGAACTTGGCTTAGGCTTCAACACCACTTTATCAATTGGTCCAATTACTAGTACTACACAAAGAGATCAACTTCAACAACAACAACAACAACAAGAAAAGACTCAAAGGGACATCAACAACAACAGTAATAGAACTGAAAATCCTAATCCTGTATTGCATCAACTTGATCTTCTTCCTCAACTCTCATTCCCATGGAATCCTCCTTCTCAAAACGGTAACAACCCACTTCCTAAAAAGTTACCAATATTTTATCTTTTACTCTTGTTTTTTCTCTTATAAAATTTAACTAGTTACAGTGATGATGAAACCAGTGTTTTTTAAATAGGATTAAAAATAGAATCAGTGCTTATTATTATTATAAGGGTTATTATTGTTTTCACAATAGGTAATTTATTATCAACCGAGTTTGGAGGTTCTTCAAGGGGATTGGATGTGAACGATGAGATGGCGCTTTCGTCGTCCCCGAATAGTGCAGCATCTTCTTTTCAGATGGATCTATGCATGTACAGTCGAAGAGGAAGTGGTGGTAGGAGTTTGAGTGGTTCAGGAGGGAACAAGAGAGAGTTTTCTGATGGTGAAGGGTATGATCAAAGGAATTCTTCAAGAGTCAGTGATGAAGATGATAACTGTGGTGTTGGTAATGGTAACACAAGGAAGAAACTTAGGCTCTCTAAGGATCAATCTGCTTTTCTTGAAGAAAGCTTCAAAGAACATCACACTCTTAACCCTGTATGTGTTTTTTTTTCTCTCTCATGTGAAGTATAAATTAGTTTATATTGTCCTCTTGAGCATAACTCAGTTTATAGACGATGCATAAAAATATGCAAAGTTGGAGTTCAAACCCCGGCCACAAAAAAAAATCTATAAAAAATACTTTCAGATTCATACCACACATACTAACATGTCGATATCAATAATAAATCTATATATGTAATGTTCCTTTCAAAAGAAAATATTCTATGTATTGTTATTGGTGTCATAAGAAACACCAAATATATCTCTAATATATAGTATTGGTGTTACAAATGTTTAAATGAATGTTTCTACTTTAGATTGAATTGAAAAAAAAATGAATGTTTAAATTTTAACACAATTTTTTTTATTATACATTTTTTTGTACTAATTTTGTAATAAGATGAAAATTTTATGACAATTCTTCCTTGGACCCATTTATTTGTTTTATTTTGTTTTTGATTTTTTGTTTAAAATACTAATGGTTGGACACAATTGTGGTGTAGAAACAAAAACTGGCTCTTGCAAAACAACTCAATCTTCGCCCTCGCCAAGTAGAAGTCTGGTTCCAAAATAGAAGAGCAAGGTACAGATACACTTTGCTAATTTTGATAAATCATACTTAGATTAAAAAAATTATAGTCAAATGCAGTGGATACAACTTTATTTTTTAAACTTTGGATATGAAAATATATAATTTAATCATATAAATTTATGTTCTGATGCAGGACTAAGTTGAAACAAACAGAAGTAGATTGTGAATACTTAAAGAGATGCTGTGAAACACTAACAGAAGAGAATAGGAGGCTACACAAGGAACTTCAAGAATTAAGAGCTTTAAAAACTTCAAATCCATTCAACATGCAATTACCAGCAACTACTTTAACTATGTGCCCTTCTTGTGAACGTGTTGCAACAAATTCTTCAGCCACTTCCTCAGTCACTAATACTAGTGCCACAATCAATGGTAATAACAATAACAAAATTAACCAGTGA
Protein sequence

>MS.gene77146.t1

MELGLSLGDSSKPLIGLMEKHPHQASKELGLGFNTTLSIGPITSTTQRDQLQQQQQQQEKTQRDINNNSNRTENPNPVLHQLDLLPQLSFPWNPPSQNGNLLSTEFGGSSRGLDVNDEMALSSSPNSAASSFQMDLCMYSRRGSGGRSLSGSGGNKREFSDGEGYDQRNSSRVSDEDDNCGVGNGNTRKKLRLSKDQSAFLEESFKEHHTLNPKQKLALAKQLNLRPRQVEVWFQNRRARTKLKQTEVDCEYLKRCCETLTEENRRLHKELQELRALKTSNPFNMQLPATTLTMCPSCERVATNSSATSSVTNTSATINGNNNNKINQ