AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77150.t1 MTR_1g019850 90.000 150 15 0 1 150 28 177 9.80e-96 276
MS.gene77150.t1 MTR_2g024230 79.000 100 20 1 1 99 47 146 5.00e-49 155
MS.gene77150.t1 MTR_0853s0010 57.627 118 43 1 40 150 22 139 2.66e-41 135
MS.gene77150.t1 MTR_1g033820 61.224 49 19 0 39 87 23 71 1.91e-17 72.8
MS.gene77150.t1 MTR_6g048540 49.333 75 30 1 38 104 23 97 6.76e-15 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77150.t1 AT4G38180 33.577 137 80 3 24 150 54 189 6.94e-11 59.7

Find 25 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCCAAAACCTTCTTTCT+TGG 0.194532 2.4:+32100866 None:intergenic
TATTTGTCCTATTGTTGATA+TGG 0.229024 2.4:-32100934 MS.gene77150:CDS
AAGCAGGGAAGAGAAGTAAA+TGG 0.304863 2.4:-32100701 MS.gene77150:CDS
AGCAGATTGGCCACACTCTT+AGG 0.344272 2.4:+32100633 None:intergenic
TTTGCCAAGAAAGAAGGTTT+TGG 0.354079 2.4:-32100870 MS.gene77150:CDS
TCAAATTCCATATCAACAAT+AGG 0.427064 2.4:+32100927 None:intergenic
GAAGGTACACATATAGTAAA+GGG 0.446132 2.4:-32100795 MS.gene77150:CDS
TCACTTACTGTTTCAAAAGC+AGG 0.452498 2.4:-32100717 MS.gene77150:CDS
ACATCGTTTGCCAAGAAAGA+AGG 0.462978 2.4:-32100876 MS.gene77150:CDS
ATAAACAATGTTCACAATCA+TGG 0.474196 2.4:-32100666 MS.gene77150:CDS
AATATTAGCTTCAGGTGACT+TGG 0.474696 2.4:+32100953 None:intergenic
ATAGGACAAATATTAGCTTC+AGG 0.478911 2.4:+32100945 None:intergenic
AGCAGGGAAGAGAAGTAAAT+GGG 0.489503 2.4:-32100700 MS.gene77150:CDS
CGAAGGTACACATATAGTAA+AGG 0.490844 2.4:-32100796 MS.gene77150:CDS
GTAAAGGGTGAAAATGAAGA+AGG 0.507431 2.4:-32100780 MS.gene77150:CDS
CTAATTATGAGAAAGCAGAT+TGG 0.522880 2.4:+32100620 None:intergenic
TGCTTTACAATCAGTTCTTG+AGG 0.537334 2.4:+32100740 None:intergenic
CACTTACTGTTTCAAAAGCA+GGG 0.542698 2.4:-32100716 MS.gene77150:CDS
GTACATGGATAATGAGTATG+TGG 0.550566 2.4:-32101057 None:intergenic
TAAAGGGTGAAAATGAAGAA+GGG 0.554329 2.4:-32100779 MS.gene77150:CDS
CAATGTTCACAATCATGGAA+TGG 0.570975 2.4:-32100661 MS.gene77150:CDS
ACACATCATCAACTATCTCA+TGG 0.582009 2.4:+32101006 None:intergenic
TATGGAATTTGACTCCATTG+TGG 0.618496 2.4:-32100916 MS.gene77150:CDS
AATGGTTAGTCCTAAGAGTG+TGG 0.676645 2.4:-32100643 MS.gene77150:CDS
ATGCTTGTATGCGCTAACGA+AGG 0.715770 2.4:-32100813 MS.gene77150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATAAACAATGTTCACAATCA+TGG - chr2.4:32101007-32101026 MS.gene77150:CDS 25.0%
! TATTTGTCCTATTGTTGATA+TGG - chr2.4:32100739-32100758 MS.gene77150:CDS 25.0%
! TCAAATTCCATATCAACAAT+AGG + chr2.4:32100749-32100768 None:intergenic 25.0%
!! GACTTGGAATTTTCTAAATT+TGG + chr2.4:32100707-32100726 None:intergenic 25.0%
CTAATTATGAGAAAGCAGAT+TGG + chr2.4:32101056-32101075 None:intergenic 30.0%
TAAAGGGTGAAAATGAAGAA+GGG - chr2.4:32100894-32100913 MS.gene77150:CDS 30.0%
! ATAGGACAAATATTAGCTTC+AGG + chr2.4:32100731-32100750 None:intergenic 30.0%
! GAAGGTACACATATAGTAAA+GGG - chr2.4:32100878-32100897 MS.gene77150:CDS 30.0%
! GAATTCTTTTACATCCACAA+TGG + chr2.4:32100774-32100793 None:intergenic 30.0%
AACTCCAAAACCTTCTTTCT+TGG + chr2.4:32100810-32100829 None:intergenic 35.0%
ACACATCATCAACTATCTCA+TGG + chr2.4:32100670-32100689 None:intergenic 35.0%
CAATGTTCACAATCATGGAA+TGG - chr2.4:32101012-32101031 MS.gene77150:CDS 35.0%
CACTTACTGTTTCAAAAGCA+GGG - chr2.4:32100957-32100976 MS.gene77150:CDS 35.0%
GTAAAGGGTGAAAATGAAGA+AGG - chr2.4:32100893-32100912 MS.gene77150:CDS 35.0%
TATGGAATTTGACTCCATTG+TGG - chr2.4:32100757-32100776 MS.gene77150:CDS 35.0%
TCACTTACTGTTTCAAAAGC+AGG - chr2.4:32100956-32100975 MS.gene77150:CDS 35.0%
TGCTTTACAATCAGTTCTTG+AGG + chr2.4:32100936-32100955 None:intergenic 35.0%
! AATATTAGCTTCAGGTGACT+TGG + chr2.4:32100723-32100742 None:intergenic 35.0%
! CGAAGGTACACATATAGTAA+AGG - chr2.4:32100877-32100896 MS.gene77150:CDS 35.0%
! TTTGCCAAGAAAGAAGGTTT+TGG - chr2.4:32100803-32100822 MS.gene77150:CDS 35.0%
AAGCAGGGAAGAGAAGTAAA+TGG - chr2.4:32100972-32100991 MS.gene77150:CDS 40.0%
ACATCGTTTGCCAAGAAAGA+AGG - chr2.4:32100797-32100816 MS.gene77150:CDS 40.0%
AGCAGGGAAGAGAAGTAAAT+GGG - chr2.4:32100973-32100992 MS.gene77150:CDS 40.0%
! AATGGTTAGTCCTAAGAGTG+TGG - chr2.4:32101030-32101049 MS.gene77150:CDS 40.0%
!!! AAGGTTTTGGAGTTCGTGTT+CGG - chr2.4:32100816-32100835 MS.gene77150:CDS 40.0%
ATGCTTGTATGCGCTAACGA+AGG - chr2.4:32100860-32100879 MS.gene77150:CDS 45.0%
! AGCAGATTGGCCACACTCTT+AGG + chr2.4:32101043-32101062 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32100620 32101075 32100620 ID=MS.gene77150
chr2.4 mRNA 32100620 32101075 32100620 ID=MS.gene77150.t1;Parent=MS.gene77150
chr2.4 exon 32100620 32101075 32100620 ID=MS.gene77150.t1.exon1;Parent=MS.gene77150.t1
chr2.4 CDS 32100620 32101075 32100620 ID=cds.MS.gene77150.t1;Parent=MS.gene77150.t1
Gene Sequence

>MS.gene77150

ATGGATAATGAGTATGTGGAAAATTCTACTGTTGAGTGTTTAGAAGACCATGAGATAGTTGATGATGTGTCTAATGATTTAGAACCAAATTTAGAAAATTCCAAGTCACCTGAAGCTAATATTTGTCCTATTGTTGATATGGAATTTGACTCCATTGTGGATGTAAAAGAATTCTACACATCGTTTGCCAAGAAAGAAGGTTTTGGAGTTCGTGTTCGGTCTACTAAGCAAATTTTTTGCATGCTTGTATGCGCTAACGAAGGTACACATATAGTAAAGGGTGAAAATGAAGAAGGGAAGAGAAAGAGTTCAACCTCAAGAACTGATTGTAAAGCATCACTTACTGTTTCAAAAGCAGGGAAGAGAAGTAAATGGGTTATAAAAAGCATAAACAATGTTCACAATCATGGAATGGTTAGTCCTAAGAGTGTGGCCAATCTGCTTTCTCATAATTAG
Protein sequence

>MS.gene77150.t1

MDNEYVENSTVECLEDHEIVDDVSNDLEPNLENSKSPEANICPIVDMEFDSIVDVKEFYTSFAKKEGFGVRVRSTKQIFCMLVCANEGTHIVKGENEEGKRKSSTSRTDCKASLTVSKAGKRSKWVIKSINNVHNHGMVSPKSVANLLSHN