AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77413

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77413.t1 MTR_7g069660 93.220 354 18 2 1 350 1 352 0.0 644
MS.gene77413.t1 MTR_2g084230 73.494 83 22 0 50 132 80 162 1.86e-37 137
MS.gene77413.t1 MTR_2g084230 73.494 83 22 0 50 132 80 162 3.73e-37 137
MS.gene77413.t1 MTR_8g086410 60.909 110 39 1 27 132 65 174 6.98e-37 137
MS.gene77413.t1 MTR_8g086410 60.909 110 39 1 27 132 67 176 7.35e-37 137
MS.gene77413.t1 MTR_2g023580 81.159 69 13 0 55 123 43 111 7.57e-35 128
MS.gene77413.t1 MTR_8g086410 57.265 117 39 2 27 132 67 183 2.84e-34 130
MS.gene77413.t1 MTR_2g016220 71.951 82 23 0 50 131 72 153 9.22e-34 128
MS.gene77413.t1 MTR_2g016220 71.951 82 23 0 50 131 72 153 9.32e-34 128
MS.gene77413.t1 MTR_2g016220 68.605 86 23 1 50 131 72 157 1.24e-31 122
MS.gene77413.t1 MTR_1g112370 72.727 66 18 0 58 123 13 78 2.74e-28 112
MS.gene77413.t1 MTR_0223s0040 65.278 72 25 0 52 123 3 74 6.00e-28 110
MS.gene77413.t1 MTR_6g071625 55.294 85 31 1 66 150 201 278 4.16e-23 99.0
MS.gene77413.t1 MTR_8g066310 44.144 111 58 1 35 141 137 247 1.86e-22 97.1
MS.gene77413.t1 MTR_1g021520 59.211 76 29 1 66 141 125 198 2.66e-22 95.5
MS.gene77413.t1 MTR_5g029470 56.098 82 35 1 61 141 212 293 6.06e-22 96.3
MS.gene77413.t1 MTR_3g450310 42.105 114 56 3 49 162 1 104 1.02e-21 92.0
MS.gene77413.t1 MTR_3g064840 64.179 67 23 1 61 126 267 333 2.98e-21 94.7
MS.gene77413.t1 MTR_7g098250 64.286 56 20 0 68 123 263 318 7.18e-19 87.8
MS.gene77413.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 1.20e-18 85.5
MS.gene77413.t1 MTR_7g093030 49.367 79 39 1 63 140 208 286 1.78e-18 84.7
MS.gene77413.t1 MTR_1g080330 62.500 56 21 0 68 123 176 231 1.95e-18 84.7
MS.gene77413.t1 MTR_6g032990 66.038 53 18 0 68 120 43 95 2.05e-18 85.9
MS.gene77413.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 2.35e-18 85.1
MS.gene77413.t1 MTR_1g080330 62.500 56 21 0 68 123 176 231 2.47e-18 85.5
MS.gene77413.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 3.02e-18 85.9
MS.gene77413.t1 MTR_5g027440 62.069 58 22 0 63 120 21 78 3.10e-18 84.0
MS.gene77413.t1 MTR_2g086450 62.500 56 21 0 68 123 46 101 3.21e-18 85.5
MS.gene77413.t1 MTR_7g093030 49.367 79 39 1 63 140 208 286 4.63e-18 84.7
MS.gene77413.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 5.63e-18 84.3
MS.gene77413.t1 MTR_7g088070 55.224 67 30 0 59 125 42 108 1.36e-17 82.0
MS.gene77413.t1 MTR_7g088070 55.224 67 30 0 59 125 42 108 1.51e-17 82.0
MS.gene77413.t1 MTR_1g090670 60.714 56 22 0 68 123 45 100 7.79e-17 80.1
MS.gene77413.t1 MTR_2g027800 60.714 56 22 0 68 123 40 95 9.13e-17 80.1
MS.gene77413.t1 MTR_7g115530 63.462 52 19 0 68 119 219 270 1.05e-16 79.7
MS.gene77413.t1 MTR_7g115530 60.714 56 22 0 68 123 219 274 1.30e-16 79.3
MS.gene77413.t1 MTR_2g027800 62.264 53 20 0 68 120 40 92 1.38e-16 79.7
MS.gene77413.t1 MTR_5g041350 56.604 53 23 0 68 120 16 68 1.87e-16 79.7
MS.gene77413.t1 MTR_1g053830 56.364 55 24 0 69 123 20 74 2.22e-16 74.3
MS.gene77413.t1 MTR_2g027860 61.404 57 22 0 69 125 51 107 2.56e-16 79.3
MS.gene77413.t1 MTR_1g053835 52.055 73 34 1 69 140 260 332 2.87e-16 79.7
MS.gene77413.t1 MTR_6g444980 55.357 56 25 0 68 123 36 91 3.40e-15 76.3
MS.gene77413.t1 MTR_6g444980 55.357 56 25 0 68 123 36 91 3.81e-15 75.9
MS.gene77413.t1 MTR_4g081710 55.357 56 25 0 68 123 18 73 5.04e-15 75.5
MS.gene77413.t1 MTR_7g068600 56.667 60 23 1 69 125 15 74 3.81e-13 68.6
MS.gene77413.t1 MTR_4g021790 51.786 56 26 1 65 120 166 220 1.42e-12 67.4
MS.gene77413.t1 MTR_1g053800 54.717 53 24 0 69 121 179 231 1.75e-12 67.8
MS.gene77413.t1 MTR_4g086835 45.205 73 31 1 57 120 255 327 3.09e-12 67.8
MS.gene77413.t1 MTR_4g064730 48.684 76 37 2 46 120 109 183 8.49e-12 65.9
MS.gene77413.t1 MTR_2g450070 39.316 117 57 6 66 178 212 318 1.08e-11 66.2
MS.gene77413.t1 MTR_2g450070 39.316 117 57 6 66 178 212 318 1.10e-11 66.2
MS.gene77413.t1 MTR_4g113140 55.769 52 23 0 69 120 237 288 1.19e-11 65.9
MS.gene77413.t1 MTR_7g093010 49.231 65 33 0 69 133 215 279 2.22e-11 64.7
MS.gene77413.t1 MTR_4g086835 45.205 73 30 2 57 120 255 326 7.09e-11 63.5
MS.gene77413.t1 MTR_5g054300 55.102 49 22 0 72 120 203 251 9.46e-11 62.8
MS.gene77413.t1 MTR_1g093080 55.102 49 22 0 72 120 216 264 9.98e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77413.t1 AT2G38300 41.718 326 154 13 44 350 32 340 3.07e-57 189
MS.gene77413.t1 AT2G40260 54.610 141 45 2 17 157 49 170 3.44e-39 144
MS.gene77413.t1 AT2G42660 78.788 66 14 0 59 124 42 107 2.08e-31 119
MS.gene77413.t1 AT2G02060 65.432 81 28 0 40 120 2 82 1.47e-29 114
MS.gene77413.t1 AT2G02060 65.432 81 28 0 40 120 2 82 1.47e-29 114
MS.gene77413.t1 AT4G04605 59.302 86 33 1 55 138 2 87 1.68e-28 110
MS.gene77413.t1 AT1G14600 68.657 67 21 0 59 125 15 81 2.21e-28 111
MS.gene77413.t1 AT4G04580 58.209 67 28 0 57 123 4 70 3.32e-23 95.1
MS.gene77413.t1 AT5G42630 67.213 61 20 0 66 126 104 164 2.36e-22 94.4
MS.gene77413.t1 AT5G42630 67.213 61 20 0 66 126 104 164 2.46e-22 95.5
MS.gene77413.t1 AT1G32240 54.878 82 36 1 61 141 206 287 9.24e-22 95.5
MS.gene77413.t1 AT1G32240 54.878 82 36 1 61 141 206 287 1.13e-21 95.1
MS.gene77413.t1 AT4G04555 60.000 65 26 0 59 123 6 70 1.52e-21 88.6
MS.gene77413.t1 AT5G16560 58.108 74 30 1 61 133 212 285 5.45e-21 93.6
MS.gene77413.t1 AT4G17695 62.295 61 23 0 66 126 163 223 4.17e-20 90.1
MS.gene77413.t1 AT5G45580 62.069 58 22 0 63 120 3 60 1.94e-18 84.3
MS.gene77413.t1 AT5G45580 62.069 58 22 0 63 120 19 76 2.31e-18 84.3
MS.gene77413.t1 AT3G04030 58.929 56 23 0 68 123 46 101 1.75e-17 83.2
MS.gene77413.t1 AT3G04030 58.929 56 23 0 68 123 46 101 1.98e-17 83.2
MS.gene77413.t1 AT3G04450 49.333 75 30 1 57 123 220 294 2.17e-17 82.0
MS.gene77413.t1 AT3G04450 49.333 75 30 1 57 123 180 254 2.60e-17 82.8
MS.gene77413.t1 AT3G04450 49.333 75 30 1 57 123 180 254 2.60e-17 82.8
MS.gene77413.t1 AT3G04450 49.333 75 30 1 57 123 220 294 2.62e-17 83.2
MS.gene77413.t1 AT5G29000 63.636 55 20 0 69 123 190 244 3.26e-17 82.4
MS.gene77413.t1 AT5G29000 63.636 55 20 0 69 123 190 244 3.26e-17 82.4
MS.gene77413.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.40e-17 82.4
MS.gene77413.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.40e-17 82.4
MS.gene77413.t1 AT5G29000 63.636 55 20 0 69 123 233 287 3.68e-17 81.3
MS.gene77413.t1 AT4G28610 61.818 55 21 0 69 123 227 281 3.76e-17 82.4
MS.gene77413.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.83e-17 82.4
MS.gene77413.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.83e-17 82.4
MS.gene77413.t1 AT5G18240 58.929 56 23 0 68 123 46 101 4.00e-17 82.4
MS.gene77413.t1 AT5G29000 63.636 55 20 0 69 123 233 287 4.54e-17 82.0
MS.gene77413.t1 AT2G01060 61.404 57 22 0 69 125 17 73 1.06e-16 79.7
MS.gene77413.t1 AT3G12730 57.143 56 24 0 68 123 24 79 1.41e-16 78.6
MS.gene77413.t1 AT4G13640 56.604 53 23 0 68 120 38 90 5.41e-16 77.8
MS.gene77413.t1 AT4G13640 56.604 53 23 0 68 120 38 90 5.65e-16 77.8
MS.gene77413.t1 AT3G24120 56.604 53 23 0 68 120 42 94 7.05e-16 77.4
MS.gene77413.t1 AT3G24120 56.604 53 23 0 68 120 42 94 7.16e-16 77.4
MS.gene77413.t1 AT3G13040 59.649 57 23 0 69 125 243 299 8.11e-16 78.6
MS.gene77413.t1 AT3G13040 59.649 57 23 0 69 125 243 299 8.11e-16 78.6
MS.gene77413.t1 AT3G13040 59.649 57 23 0 69 125 243 299 8.11e-16 78.6
MS.gene77413.t1 AT1G79430 55.357 56 25 0 68 123 35 90 1.84e-15 77.0
MS.gene77413.t1 AT2G20400 57.895 57 24 0 69 125 233 289 1.94e-15 77.4
MS.gene77413.t1 AT2G20400 57.895 57 24 0 69 125 233 289 1.95e-15 77.4
MS.gene77413.t1 AT5G06800 54.098 61 28 0 69 129 194 254 3.20e-15 75.5
MS.gene77413.t1 AT3G04030 57.143 56 23 1 68 123 46 100 4.93e-15 75.9
MS.gene77413.t1 AT1G69580 55.357 56 25 0 68 123 35 90 6.59e-15 75.1
MS.gene77413.t1 AT1G69580 55.357 56 25 0 68 123 35 90 7.10e-15 75.1
MS.gene77413.t1 AT5G06800 54.098 61 28 0 69 129 194 254 7.56e-15 75.5
MS.gene77413.t1 AT5G06800 54.098 61 28 0 69 129 194 254 7.92e-15 75.1
MS.gene77413.t1 AT2G06020 36.522 115 62 3 15 120 27 139 5.34e-14 72.0
MS.gene77413.t1 AT5G59570 45.455 88 44 3 35 120 108 193 2.68e-13 70.1
MS.gene77413.t1 AT5G59570 45.455 88 44 3 35 120 108 193 2.68e-13 70.1
MS.gene77413.t1 AT2G40260 58.929 56 21 1 102 157 1 54 2.00e-12 67.4
MS.gene77413.t1 AT1G25550 59.184 49 20 0 72 120 214 262 2.22e-12 67.8
MS.gene77413.t1 AT2G03500 57.143 49 21 0 72 120 238 286 1.09e-11 66.2
MS.gene77413.t1 AT1G25550 46.269 67 32 1 72 138 214 276 1.45e-11 64.7
MS.gene77413.t1 AT3G46640 54.545 55 24 1 66 120 143 196 1.97e-11 64.7
MS.gene77413.t1 AT3G46640 54.545 55 24 1 66 120 143 196 1.97e-11 64.7
MS.gene77413.t1 AT3G46640 54.545 55 24 1 66 120 143 196 2.99e-11 64.3
MS.gene77413.t1 AT3G62670 43.056 72 34 2 53 120 200 268 3.47e-11 64.7

Find 58 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTGAAGAACCAATTT+TGG 0.129269 7.3:-35299569 None:intergenic
TGTTGATAGTTCTCTAAAAT+TGG 0.214021 7.3:-35300644 None:intergenic
GATATTGATGCTGCTATTAT+TGG 0.224571 7.3:-35300590 None:intergenic
CTTCAATTGATGAACATAAA+AGG 0.228877 7.3:+35299581 MS.gene77413:CDS
GAAAGGAGTCAATATTTCTT+TGG 0.270906 7.3:+35301002 MS.gene77413:CDS
CTATATGATGATGTTTGATT+TGG 0.300830 7.3:-35299990 None:intergenic
GGAAATTATCATTTGGTTCA+TGG 0.310661 7.3:-35300675 None:intergenic
GAAAAGAAAAGCTTCAAATA+TGG 0.323316 7.3:+35300813 MS.gene77413:CDS
TCATGTGAAGAGTCATTTAC+AGG 0.340032 7.3:+35299616 MS.gene77413:CDS
TGTAGGAGCAACTCCAAAAT+TGG 0.369328 7.3:+35299556 MS.gene77413:intron
TAGAATATGTCTCATTCATA+TGG 0.376004 7.3:-35301031 None:intergenic
AGAAGAACAAGATAGTTCTA+AGG 0.388489 7.3:+35300972 MS.gene77413:CDS
GCCTTGAAGCATGGGAAGTT+GGG 0.390047 7.3:-35299961 None:intergenic
ATTAATTGAAACACTTGCCT+TGG 0.402233 7.3:-35299051 None:intergenic
TATCACTAAAATTAAATTCA+AGG 0.404007 7.3:+35300851 MS.gene77413:CDS
GATCATCTCTTAAAGATTCA+TGG 0.404027 7.3:-35300711 None:intergenic
AATGAGAGGAAATTATCATT+TGG 0.404536 7.3:-35300682 None:intergenic
TAAAATTGGATGAATCCATT+TGG 0.405764 7.3:-35300630 None:intergenic
CTTCATGCTGTGCAAAGACT+TGG 0.407098 7.3:+35299251 MS.gene77413:CDS
ACCCAACTTCCCATGCTTCA+AGG 0.422458 7.3:+35299960 MS.gene77413:CDS
GAATCTAGAGCTGAGTTCTA+TGG 0.429313 7.3:+35300550 MS.gene77413:CDS
CAAATCAAACATCATCATAT+AGG 0.434083 7.3:+35299991 MS.gene77413:CDS
TTTACAGATGTGGATCATAT+GGG 0.447788 7.3:+35300476 MS.gene77413:intron
AATCATCTTATGTTTGCAAA+AGG 0.448039 7.3:+35300944 MS.gene77413:CDS
TAACAGTGTTGGCTGATCAT+AGG 0.450325 7.3:+35299898 MS.gene77413:intron
GAAATGAGATCTTGTGCTCT+TGG 0.453092 7.3:-35300745 None:intergenic
TAATAGCAGCATCAATATCC+AGG 0.455941 7.3:+35300594 MS.gene77413:CDS
AGAAAACGAAAATGAAGATC+AGG 0.462194 7.3:-35299230 None:intergenic
CCAACGTAGCCTAGGCAACT+TGG 0.470061 7.3:-35299204 None:intergenic
AGCCTTGAAGCATGGGAAGT+TGG 0.470813 7.3:-35299962 None:intergenic
GGAGTATAGCCTTGAAGCAT+GGG 0.488714 7.3:-35299969 None:intergenic
ATGAGAGAGGAAGAAACATA+TGG 0.490280 7.3:+35299017 MS.gene77413:CDS
TTCAAATATGGATCTTGAGT+TGG 0.495575 7.3:+35300825 MS.gene77413:CDS
CATGCTGTGCAAAGACTTGG+TGG 0.512111 7.3:+35299254 MS.gene77413:CDS
TGGAGTATAGCCTTGAAGCA+TGG 0.512500 7.3:-35299970 None:intergenic
GGCAACTTGGATCTAACATA+AGG 0.518664 7.3:-35299191 None:intergenic
ATGTTAGATCCAAGTTGCCT+AGG 0.523365 7.3:+35299195 MS.gene77413:CDS
AGTTCTAAGGAGCAAGAGAA+AGG 0.525178 7.3:+35300985 MS.gene77413:CDS
AAAGTAGTTGACACAAATCA+AGG 0.545661 7.3:+35299772 MS.gene77413:CDS
TGGTGATCATAAATGTATAG+AGG 0.550152 7.3:+35300879 MS.gene77413:CDS
TTTGGAAAATACTATGACCC+TGG 0.552012 7.3:-35300612 None:intergenic
TCAGGAGTCCAACGTAGCCT+AGG 0.559435 7.3:-35299212 None:intergenic
CCAAGTTGCCTAGGCTACGT+TGG 0.561524 7.3:+35299204 MS.gene77413:CDS
TCTTGCCATGTATGAAAACA+TGG 0.579727 7.3:+35300507 MS.gene77413:CDS
CATAGGCTTCTTGTAGACAA+TGG 0.581433 7.3:+35299915 MS.gene77413:CDS
CTAACATAAGGCCTCACTGA+TGG 0.585574 7.3:-35299179 None:intergenic
TTACAGATGTGGATCATATG+GGG 0.594097 7.3:+35300477 MS.gene77413:intron
AAATTAAATTCAAGGAGCAA+TGG 0.605129 7.3:+35300859 MS.gene77413:CDS
TAACTTGCCTGAAAATCAAG+AGG 0.607982 7.3:+35300774 MS.gene77413:CDS
ATTCATGGCCACAAAATGAG+AGG 0.620365 7.3:-35300696 None:intergenic
GATGTGCAAAAGAACAACAA+TGG 0.621747 7.3:+35299107 MS.gene77413:CDS
AATAGCAGCATCAATATCCA+GGG 0.637864 7.3:+35300595 MS.gene77413:CDS
TATGATTGTGAAATTAGTGA+AGG 0.638841 7.3:+35299077 MS.gene77413:CDS
ATATGGATCAGAATGTTCCA+AGG 0.643313 7.3:+35299034 MS.gene77413:CDS
GTGCAAAAGAACAACAATGG+AGG 0.658548 7.3:+35299110 MS.gene77413:CDS
TAGCTAGATGAATATGAGAG+AGG 0.659511 7.3:+35299004 None:intergenic
ATGCTGTGCAAAGACTTGGT+GGG 0.679037 7.3:+35299255 MS.gene77413:CDS
GTGAGAAGAAACCATCAGTG+AGG 0.735513 7.3:+35299168 MS.gene77413:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTAATTTTATTTTT+TGG - chr7.3:35300293-35300312 None:intergenic 0.0%
!! AATAAGTGATTACATAATTA+GGG + chr7.3:35299833-35299852 MS.gene77413:intron 15.0%
!! TAAAAAGTCATAGATAAATA+TGG + chr7.3:35299639-35299658 MS.gene77413:intron 15.0%
!! TATCACTAAAATTAAATTCA+AGG + chr7.3:35300851-35300870 MS.gene77413:CDS 15.0%
!!! TGAATTAACCATTTTTTTTA+TGG + chr7.3:35300029-35300048 MS.gene77413:intron 15.0%
!! AGTTATCAAAACAAATACAA+AGG + chr7.3:35300240-35300259 MS.gene77413:intron 20.0%
!! CAATAAGTGATTACATAATT+AGG + chr7.3:35299832-35299851 MS.gene77413:intron 20.0%
!! GAAAAATTGAAATCTAGAAA+AGG - chr7.3:35299418-35299437 None:intergenic 20.0%
!! GTTATCAAAACAAATACAAA+GGG + chr7.3:35300241-35300260 MS.gene77413:intron 20.0%
!!! AAGACCTTTTTTTATTCATA+GGG + chr7.3:35300181-35300200 MS.gene77413:intron 20.0%
!!! CTAGATTTCAATTTTTCATA+GGG + chr7.3:35299421-35299440 MS.gene77413:intron 20.0%
!!! TAGATTTCAATTTTTCATAG+GGG + chr7.3:35299422-35299441 MS.gene77413:intron 20.0%
!!! TCTAGATTTCAATTTTTCAT+AGG + chr7.3:35299420-35299439 MS.gene77413:intron 20.0%
!!! TTTTAAACGTAAATGACTTA+AGG - chr7.3:35300368-35300387 None:intergenic 20.0%
!!! TTTTATTTTTTGGTCAAGTT+CGG - chr7.3:35300283-35300302 None:intergenic 20.0%
!!! TTTTCTTTTTTTTACAGATG+TGG + chr7.3:35300466-35300485 MS.gene77413:intron 20.0%
! AAATTAAATTCAAGGAGCAA+TGG + chr7.3:35300859-35300878 MS.gene77413:CDS 25.0%
! AATCATCTTATGTTTGCAAA+AGG + chr7.3:35300944-35300963 MS.gene77413:CDS 25.0%
! AATGAGAGGAAATTATCATT+TGG - chr7.3:35300685-35300704 None:intergenic 25.0%
! AGCATTCTCCATAAAAAAAA+TGG - chr7.3:35300040-35300059 None:intergenic 25.0%
! ATACATATATGTTATGTGTG+TGG - chr7.3:35299295-35299314 None:intergenic 25.0%
! CAAATCAAACATCATCATAT+AGG + chr7.3:35299991-35300010 MS.gene77413:CDS 25.0%
! CTTCAATTGATGAACATAAA+AGG + chr7.3:35299581-35299600 MS.gene77413:CDS 25.0%
! GAAAAGAAAAGCTTCAAATA+TGG + chr7.3:35300813-35300832 MS.gene77413:CDS 25.0%
! GTAAATGACTTAAGGAAATT+TGG - chr7.3:35300360-35300379 None:intergenic 25.0%
! TAAAATTGGATGAATCCATT+TGG - chr7.3:35300633-35300652 None:intergenic 25.0%
! TATGATTGTGAAATTAGTGA+AGG + chr7.3:35299077-35299096 MS.gene77413:CDS 25.0%
! TGATCCCTATGAATAAAAAA+AGG - chr7.3:35300188-35300207 None:intergenic 25.0%
! TGTTATTGTCTTAGAATTGA+AGG + chr7.3:35300441-35300460 MS.gene77413:intron 25.0%
! TTGTATATATGTAACAGTGT+TGG + chr7.3:35299887-35299906 MS.gene77413:intron 25.0%
!! ATCAATTGAAGAACCAATTT+TGG - chr7.3:35299572-35299591 None:intergenic 25.0%
!! CTATATGATGATGTTTGATT+TGG - chr7.3:35299993-35300012 None:intergenic 25.0%
!! TTAATGATTTTCTTGTGTGT+AGG + chr7.3:35299539-35299558 MS.gene77413:intron 25.0%
!!! CAAGACCTTTTTTTATTCAT+AGG + chr7.3:35300180-35300199 MS.gene77413:intron 25.0%
!!! TGTTGATAGTTCTCTAAAAT+TGG - chr7.3:35300647-35300666 None:intergenic 25.0%
AAAGCTAGCTAGCTATATAT+AGG - chr7.3:35299671-35299690 None:intergenic 30.0%
AAAGTAGTTGACACAAATCA+AGG + chr7.3:35299772-35299791 MS.gene77413:CDS 30.0%
ACACAAATCAAGGTACAATA+AGG + chr7.3:35299782-35299801 MS.gene77413:intron 30.0%
AGAAAACGAAAATGAAGATC+AGG - chr7.3:35299233-35299252 None:intergenic 30.0%
ATCTATGTGTTTGATCATCA+TGG - chr7.3:35299381-35299400 None:intergenic 30.0%
ATTAATTGAAACACTTGCCT+TGG - chr7.3:35299054-35299073 None:intergenic 30.0%
ATTCTTATCGAAAAACGAGA+AGG - chr7.3:35300218-35300237 None:intergenic 30.0%
GAAAGGAGTCAATATTTCTT+TGG + chr7.3:35301002-35301021 MS.gene77413:CDS 30.0%
GATATTGATGCTGCTATTAT+TGG - chr7.3:35300593-35300612 None:intergenic 30.0%
GATCATCTCTTAAAGATTCA+TGG - chr7.3:35300714-35300733 None:intergenic 30.0%
GTAACCTAGAATTTACAACA+TGG + chr7.3:35299349-35299368 MS.gene77413:intron 30.0%
TGGTGATCATAAATGTATAG+AGG + chr7.3:35300879-35300898 MS.gene77413:CDS 30.0%
TTCAAATATGGATCTTGAGT+TGG + chr7.3:35300825-35300844 MS.gene77413:CDS 30.0%
TTCAATATCAAGTTAGTCCT+AGG + chr7.3:35299447-35299466 MS.gene77413:intron 30.0%
TTCTTATCGAAAAACGAGAA+GGG - chr7.3:35300217-35300236 None:intergenic 30.0%
TTTACAGATGTGGATCATAT+GGG + chr7.3:35300476-35300495 MS.gene77413:intron 30.0%
! AAGGAAATTTGGAACAAAGT+TGG - chr7.3:35300349-35300368 None:intergenic 30.0%
! AGAAGAACAAGATAGTTCTA+AGG + chr7.3:35300972-35300991 MS.gene77413:CDS 30.0%
! AGTAATTTTACAACTGTCGT+TGG - chr7.3:35300422-35300441 None:intergenic 30.0%
! CGTTTAAAATTGTAACCTCA+AGG + chr7.3:35300379-35300398 MS.gene77413:intron 30.0%
! GATAATTTCCTCTCATTTTG+TGG + chr7.3:35300688-35300707 MS.gene77413:CDS 30.0%
! GATTTGAAGTTTAAACCTTG+AGG - chr7.3:35300397-35300416 None:intergenic 30.0%
! GGAAATTATCATTTGGTTCA+TGG - chr7.3:35300678-35300697 None:intergenic 30.0%
! TTGTACCATGTTTTCATACA+TGG - chr7.3:35300515-35300534 None:intergenic 30.0%
! TTTTACAGATGTGGATCATA+TGG + chr7.3:35300475-35300494 MS.gene77413:intron 30.0%
AATAGCAGCATCAATATCCA+GGG + chr7.3:35300595-35300614 MS.gene77413:CDS 35.0%
ATATCAAGTTAGTCCTAGGT+AGG + chr7.3:35299451-35299470 MS.gene77413:intron 35.0%
ATATGGATCAGAATGTTCCA+AGG + chr7.3:35299034-35299053 MS.gene77413:CDS 35.0%
ATGAGAGAGGAAGAAACATA+TGG + chr7.3:35299017-35299036 MS.gene77413:CDS 35.0%
CACACCATGTTGTAAATTCT+AGG - chr7.3:35299356-35299375 None:intergenic 35.0%
CTAGAATTTACAACATGGTG+TGG + chr7.3:35299354-35299373 MS.gene77413:intron 35.0%
CTTTCTTGCTTCTATACATC+TGG - chr7.3:35299756-35299775 None:intergenic 35.0%
GATGTGCAAAAGAACAACAA+TGG + chr7.3:35299107-35299126 MS.gene77413:CDS 35.0%
TAATAGCAGCATCAATATCC+AGG + chr7.3:35300594-35300613 MS.gene77413:CDS 35.0%
TCATGTGAAGAGTCATTTAC+AGG + chr7.3:35299616-35299635 MS.gene77413:CDS 35.0%
TCTTGCCATGTATGAAAACA+TGG + chr7.3:35300507-35300526 MS.gene77413:CDS 35.0%
TTACAGATGTGGATCATATG+GGG + chr7.3:35300477-35300496 MS.gene77413:intron 35.0%
TTTGGAAAATACTATGACCC+TGG - chr7.3:35300615-35300634 None:intergenic 35.0%
! AATTTTACAACTGTCGTTGG+AGG - chr7.3:35300419-35300438 None:intergenic 35.0%
! AGTTCTTCCTCTTGATTTTC+AGG - chr7.3:35300784-35300803 None:intergenic 35.0%
! GGGTCATAGTATTTTCCAAA+TGG + chr7.3:35300615-35300634 MS.gene77413:CDS 35.0%
! TAACTTGCCTGAAAATCAAG+AGG + chr7.3:35300774-35300793 MS.gene77413:CDS 35.0%
ATCTTTAACCATTACCCCTG+AGG + chr7.3:35300146-35300165 MS.gene77413:intron 40.0%
ATGTTAGATCCAAGTTGCCT+AGG + chr7.3:35299195-35299214 MS.gene77413:CDS 40.0%
ATTCATGGCCACAAAATGAG+AGG - chr7.3:35300699-35300718 None:intergenic 40.0%
CATAGGCTTCTTGTAGACAA+TGG + chr7.3:35299915-35299934 MS.gene77413:CDS 40.0%
GAAATGAGATCTTGTGCTCT+TGG - chr7.3:35300748-35300767 None:intergenic 40.0%
GGCAACTTGGATCTAACATA+AGG - chr7.3:35299194-35299213 None:intergenic 40.0%
GTGCAAAAGAACAACAATGG+AGG + chr7.3:35299110-35299129 MS.gene77413:CDS 40.0%
TCTTTAACCATTACCCCTGA+GGG + chr7.3:35300147-35300166 MS.gene77413:intron 40.0%
TGTAGGAGCAACTCCAAAAT+TGG + chr7.3:35299556-35299575 MS.gene77413:intron 40.0%
TTGAATTGACCTACCTACCT+AGG - chr7.3:35299467-35299486 None:intergenic 40.0%
! GAATCTAGAGCTGAGTTCTA+TGG + chr7.3:35300550-35300569 MS.gene77413:CDS 40.0%
!! AGTTCTAAGGAGCAAGAGAA+AGG + chr7.3:35300985-35301004 MS.gene77413:CDS 40.0%
!! TAACAGTGTTGGCTGATCAT+AGG + chr7.3:35299898-35299917 MS.gene77413:intron 40.0%
AATGCTAACTAGTGCCCCAA+GGG + chr7.3:35300054-35300073 MS.gene77413:intron 45.0%
CAAGTTAGTCCTAGGTAGGT+AGG + chr7.3:35299455-35299474 MS.gene77413:intron 45.0%
CTAACATAAGGCCTCACTGA+TGG - chr7.3:35299182-35299201 None:intergenic 45.0%
CTTAACCAATGCATGCCCTT+GGG - chr7.3:35300072-35300091 None:intergenic 45.0%
CTTCATGCTGTGCAAAGACT+TGG + chr7.3:35299251-35299270 MS.gene77413:CDS 45.0%
GTGAGAAGAAACCATCAGTG+AGG + chr7.3:35299168-35299187 MS.gene77413:CDS 45.0%
TTAACCAATGCATGCCCTTG+GGG - chr7.3:35300071-35300090 None:intergenic 45.0%
! ATGCTGTGCAAAGACTTGGT+GGG + chr7.3:35299255-35299274 MS.gene77413:CDS 45.0%
! GGAGTATAGCCTTGAAGCAT+GGG - chr7.3:35299972-35299991 None:intergenic 45.0%
! TGGAGTATAGCCTTGAAGCA+TGG - chr7.3:35299973-35299992 None:intergenic 45.0%
ACCCAACTTCCCATGCTTCA+AGG + chr7.3:35299960-35299979 MS.gene77413:CDS 50.0%
AGCCTTGAAGCATGGGAAGT+TGG - chr7.3:35299965-35299984 None:intergenic 50.0%
CATGCTGTGCAAAGACTTGG+TGG + chr7.3:35299254-35299273 MS.gene77413:CDS 50.0%
GAATGCTAACTAGTGCCCCA+AGG + chr7.3:35300053-35300072 MS.gene77413:intron 50.0%
GCCTTGAAGCATGGGAAGTT+GGG - chr7.3:35299964-35299983 None:intergenic 50.0%
GCTTAACCAATGCATGCCCT+TGG - chr7.3:35300073-35300092 None:intergenic 50.0%
CCAACGTAGCCTAGGCAACT+TGG - chr7.3:35299207-35299226 None:intergenic 55.0%
CCAAGTTGCCTAGGCTACGT+TGG + chr7.3:35299204-35299223 MS.gene77413:CDS 55.0%
CTTGTTAACCAGCGCCCTCA+GGG - chr7.3:35300164-35300183 None:intergenic 55.0%
TCAGGAGTCCAACGTAGCCT+AGG - chr7.3:35299215-35299234 None:intergenic 55.0%
TCTTGTTAACCAGCGCCCTC+AGG - chr7.3:35300165-35300184 None:intergenic 55.0%
TTGTTAACCAGCGCCCTCAG+GGG - chr7.3:35300163-35300182 None:intergenic 55.0%
! AGTGCCCCAAGGGCATGCAT+TGG + chr7.3:35300064-35300083 MS.gene77413:intron 60.0%
ACCAGCGCCCTCAGGGGTAA+TGG - chr7.3:35300157-35300176 None:intergenic 65.0%
! ACCATTACCCCTGAGGGCGC+TGG + chr7.3:35300153-35300172 MS.gene77413:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 35299011 35301038 35299011 ID=MS.gene77413
chr7.3 mRNA 35299011 35301038 35299011 ID=MS.gene77413.t1;Parent=MS.gene77413
chr7.3 exon 35299011 35299284 35299011 ID=MS.gene77413.t1.exon1;Parent=MS.gene77413.t1
chr7.3 CDS 35299011 35299284 35299011 ID=cds.MS.gene77413.t1;Parent=MS.gene77413.t1
chr7.3 exon 35299561 35299637 35299561 ID=MS.gene77413.t1.exon2;Parent=MS.gene77413.t1
chr7.3 CDS 35299561 35299637 35299561 ID=cds.MS.gene77413.t1;Parent=MS.gene77413.t1
chr7.3 exon 35299757 35299793 35299757 ID=MS.gene77413.t1.exon3;Parent=MS.gene77413.t1
chr7.3 CDS 35299757 35299793 35299757 ID=cds.MS.gene77413.t1;Parent=MS.gene77413.t1
chr7.3 exon 35299904 35300012 35299904 ID=MS.gene77413.t1.exon4;Parent=MS.gene77413.t1
chr7.3 CDS 35299904 35300012 35299904 ID=cds.MS.gene77413.t1;Parent=MS.gene77413.t1
chr7.3 exon 35300483 35301038 35300483 ID=MS.gene77413.t1.exon5;Parent=MS.gene77413.t1
chr7.3 CDS 35300483 35301038 35300483 ID=cds.MS.gene77413.t1;Parent=MS.gene77413.t1
Gene Sequence

>MS.gene77413

ATGAATATGAGAGAGGAAGAAACATATGGATCAGAATGTTCCAAGGCAAGTGTTTCAATTAATGAATATGATTGTGAAATTAGTGAAGGAAATGAAGATGTGCAAAAGAACAACAATGGAGGAATAAGCTCAAGCAATAGTACTATAGAAGAGAATTGTGAGAAGAAACCATCAGTGAGGCCTTATGTTAGATCCAAGTTGCCTAGGCTACGTTGGACTCCTGATCTTCATTTTCGTTTTCTTCATGCTGTGCAAAGACTTGGTGGGCAAGAGAGTAAGTTCCACACACATAACATATATGTATATTTTTCTTATAATTATTAGCTAAGAATTGAAAAGTAACCTAGAATTTACAACATGGTGTGGACCATGATGATCAAACACATAGATATAGCTTCTTTTTTCCTTTTCTAGATTTCAATTTTTCATAGGGGATTTCAATATCAAGTTAGTCCTAGGTAGGTAGGTCAATTCAACAATATGATTATTCTTGTTATTTTAATTTTAATTTTAATTTTGTATGTATTGTTAATGATTTTCTTGTGTGTAGGAGCAACTCCAAAATTGGTTCTTCAATTGATGAACATAAAAGGTTTAAGTATTGCTCATGTGAAGAGTCATTTACAGGTAAAAAGTCATAGATAAATATGGTTGCAACCTATATATAGCTAGCTAGCTTTCAATATTTCTTAATATGTATAATTGTTTAATTAATTTCTTTTCTTTCACATGTATTTCTTTTCCAGATGTATAGAAGCAAGAAAGTAGTTGACACAAATCAAGGTACAATAAGGTTTTCTTTTTTGTATGAATTCAACTATCAATAAGTGATTACATAATTAGGGTTTGTGCTTGCAAGTCACTAATTATTTATGATTGTATATATGTAACAGTGTTGGCTGATCATAGGCTTCTTGTAGACAATGGAGACAGAAATGTTTATAATCTCACCCAACTTCCCATGCTTCAAGGCTATACTCCAAATCAAACATCATCATATAGGTATACATATACTACTTGAATTAACCATTTTTTTTATGGAGAATGCTAACTAGTGCCCCAAGGGCATGCATTGGTTAAGCATCTAATCAAAAGTAACATCTTATATTTCAAAGACAAAAATTCTATTTGTAGAATCTTTAACCATTACCCCTGAGGGCGCTGGTTAACAAGACCTTTTTTTATTCATAGGGATCATATAAACCCTTCTCGTTTTTCGATAAGAATAAAGTTATCAAAACAAATACAAAGGGTACTAAAATCGAGTTACCGAACTTGACCAAAAAATAAAATTAAAAAAATCGAGTTACACAAGAACAAAATACGAGTTGCTAACCAACTTTGTTCCAAATTTCCTTAAGTCATTTACGTTTAAAATTGTAACCTCAAGGTTTAAACTTCAAATCCTCCAACGACAGTTGTAAAATTACTGTTATTGTCTTAGAATTGAAGGTTTTTTCTTTTTTTTACAGATGTGGATCATATGGGGATGCTTCTCTTGCCATGTATGAAAACATGGTACAAATGAGTTCAATAAGTGAATCTAGAGCTGAGTTCTATGGCAAAATGATTGAGAGAACCAATAATAGCAGCATCAATATCCAGGGTCATAGTATTTTCCAAATGGATTCATCCAATTTTAGAGAACTATCAACAACTAAAGTCCATGAACCAAATGATAATTTCCTCTCATTTTGTGGCCATGAATCTTTAAGAGATGATCTACAAGTTCAGCCAAGAGCACAAGATCTCATTTCGAACGATAACTTGCCTGAAAATCAAGAGGAAGAACTGAAGACAATGAAAAGAAAAGCTTCAAATATGGATCTTGAGTTGGATCTATCACTAAAATTAAATTCAAGGAGCAATGGTGATCATAAATGTATAGAGGAGCATGAAATTGATAGCAATTTATCACTCTCTTTGTATTCTCAATCATCTTATGTTTGCAAAAGGATGAAAGAAGAACAAGATAGTTCTAAGGAGCAAGAGAAAGGAGTCAATATTTCTTTGGATCTAACCATATGA
Protein sequence

>MS.gene77413.t1

MNMREEETYGSECSKASVSINEYDCEISEGNEDVQKNNNGGISSSNSTIEENCEKKPSVRPYVRSKLPRLRWTPDLHFRFLHAVQRLGGQERATPKLVLQLMNIKGLSIAHVKSHLQMYRSKKVVDTNQVLADHRLLVDNGDRNVYNLTQLPMLQGYTPNQTSSYRCGSYGDASLAMYENMVQMSSISESRAEFYGKMIERTNNSSINIQGHSIFQMDSSNFRELSTTKVHEPNDNFLSFCGHESLRDDLQVQPRAQDLISNDNLPENQEEELKTMKRKASNMDLELDLSLKLNSRSNGDHKCIEEHEIDSNLSLSLYSQSSYVCKRMKEEQDSSKEQEKGVNISLDLTI