Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77688.t1 | XP_012464685.1 | 52.2 | 92 | 27 | 3 | 7 | 82 | 226 | 316 | 6.00E-11 | 77.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77688.t1 | Q9LJL9 | 82.5 | 40 | 7 | 0 | 7 | 46 | 240 | 279 | 2.1e-12 | 73.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77688.t1 | A0A1U8KYM7 | 52.2 | 92 | 27 | 3 | 7 | 82 | 226 | 316 | 4.3e-11 | 77.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77688.t1 | MTR_4g086660 | 87.500 | 40 | 5 | 0 | 7 | 46 | 233 | 272 | 9.99e-17 | 75.9 |
| MS.gene77688.t1 | MTR_4g088560 | 74.419 | 43 | 11 | 0 | 90 | 132 | 117 | 159 | 5.25e-15 | 68.2 |
| MS.gene77688.t1 | MTR_8g079790 | 80.000 | 40 | 8 | 0 | 7 | 46 | 224 | 263 | 6.67e-15 | 70.9 |
| MS.gene77688.t1 | MTR_3g092230 | 70.000 | 40 | 12 | 0 | 7 | 46 | 234 | 273 | 1.17e-13 | 67.4 |
| MS.gene77688.t1 | MTR_5g017830 | 70.000 | 40 | 12 | 0 | 7 | 46 | 230 | 269 | 2.68e-13 | 66.2 |
| MS.gene77688.t1 | MTR_7g118020 | 72.973 | 37 | 10 | 0 | 7 | 43 | 240 | 276 | 8.15e-13 | 64.7 |
| MS.gene77688.t1 | MTR_7g089480 | 67.500 | 40 | 13 | 0 | 7 | 46 | 237 | 276 | 1.20e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77688.t1 | AT3G19100 | 82.500 | 40 | 7 | 0 | 7 | 46 | 240 | 279 | 5.27e-16 | 73.9 |
| MS.gene77688.t1 | AT3G50530 | 77.500 | 40 | 9 | 0 | 7 | 46 | 244 | 283 | 5.25e-15 | 71.2 |
| MS.gene77688.t1 | AT3G50530 | 77.500 | 40 | 9 | 0 | 7 | 46 | 244 | 283 | 5.26e-15 | 71.2 |
| MS.gene77688.t1 | AT2G41140 | 80.000 | 40 | 8 | 0 | 7 | 46 | 219 | 258 | 8.31e-15 | 70.5 |
| MS.gene77688.t1 | AT1G49580 | 75.000 | 40 | 10 | 0 | 7 | 46 | 246 | 285 | 4.25e-14 | 68.6 |
| MS.gene77688.t1 | AT2G46700 | 75.000 | 40 | 10 | 0 | 7 | 46 | 58 | 97 | 8.31e-14 | 67.4 |
| MS.gene77688.t1 | AT2G46700 | 75.000 | 40 | 10 | 0 | 7 | 46 | 58 | 97 | 8.31e-14 | 67.4 |
| MS.gene77688.t1 | AT2G46700 | 81.081 | 37 | 7 | 0 | 7 | 43 | 239 | 275 | 8.49e-14 | 67.8 |
| MS.gene77688.t1 | AT3G56760 | 75.000 | 40 | 10 | 0 | 7 | 46 | 220 | 259 | 2.69e-13 | 66.2 |
Find 24 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCCAACAGTCACGTTTGTT+GGG | 0.299044 | 4.4:-67599621 | None:intergenic |
| AACTCTTCATTTGCGACTGT+TGG | 0.374249 | 4.4:+67599527 | MS.gene77688:CDS |
| CATGATCCATGCACTTCTAT+TGG | 0.388803 | 4.4:-67599010 | None:intergenic |
| CCCCAACAAACGTGACTGTT+GGG | 0.392125 | 4.4:+67599620 | MS.gene77688:CDS |
| TCCCCAACAGTCACGTTTGT+TGG | 0.397482 | 4.4:-67599622 | None:intergenic |
| GCCGCTCCAACTGCCGCAAA+AGG | 0.436342 | 4.4:-67599564 | None:intergenic |
| ACTCTTCATTTGCGACTGTT+GGG | 0.448333 | 4.4:+67599528 | MS.gene77688:CDS |
| ACCCCAACAAACGTGACTGT+TGG | 0.491561 | 4.4:+67599619 | MS.gene77688:CDS |
| CCCAACAGTCACGTTTGTTG+GGG | 0.500328 | 4.4:-67599620 | None:intergenic |
| TCAACAACAGAGTGAAACTT+TGG | 0.514255 | 4.4:-67598967 | None:intergenic |
| ATTTATTGCTTCTTCAGAGG+AGG | 0.547525 | 4.4:+67598725 | MS.gene77688:CDS |
| TATTTGTACCATGACAGCCT+TGG | 0.549299 | 4.4:-67598768 | None:intergenic |
| ATGATTTATTGCTTCTTCAG+AGG | 0.551860 | 4.4:+67598722 | MS.gene77688:CDS |
| AGATGATGCCAAGGCTGTCA+TGG | 0.554908 | 4.4:+67598760 | MS.gene77688:CDS |
| TTTATTGCTTCTTCAGAGGA+GGG | 0.559234 | 4.4:+67598726 | MS.gene77688:CDS |
| TGAAACTTTGGTGTAAGCAT+AGG | 0.579401 | 4.4:-67598955 | None:intergenic |
| AAAGTTTCACTCTGTTGTTG+AGG | 0.582670 | 4.4:+67598969 | MS.gene77688:CDS |
| TGGAGCGGCTGTTCCACGAA+GGG | 0.587349 | 4.4:+67599578 | MS.gene77688:CDS |
| CCCAACAAACGTGACTGTTG+GGG | 0.598159 | 4.4:+67599621 | MS.gene77688:CDS |
| TTGGAGCGGCTGTTCCACGA+AGG | 0.600775 | 4.4:+67599577 | MS.gene77688:CDS |
| ATACTCAGAAGATGATGCCA+AGG | 0.618920 | 4.4:+67598751 | MS.gene77688:CDS |
| GCGGTACCAATAGAAGTGCA+TGG | 0.619785 | 4.4:+67599004 | MS.gene77688:CDS |
| GTTGAGGATAGCAGCAGATG+CGG | 0.641502 | 4.4:+67598985 | MS.gene77688:CDS |
| GCACATAGATCTTAAGCCTG+AGG | 0.656272 | 4.4:+67598829 | MS.gene77688:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATATATTTATTTAAAT+TGG | + | chr4.4:67599274-67599293 | MS.gene77688:intron | 0.0% |
| !!! | TAATTTTTTAAAAAAAAAAA+TGG | - | chr4.4:67599457-67599476 | None:intergenic | 0.0% |
| !! | AAATAAAATAAAATTGAATG+GGG | - | chr4.4:67599312-67599331 | None:intergenic | 10.0% |
| !! | AATCATATTTAAATATCTTA+GGG | + | chr4.4:67598910-67598929 | MS.gene77688:intron | 10.0% |
| !! | TAATCATATTTAAATATCTT+AGG | + | chr4.4:67598909-67598928 | MS.gene77688:intron | 10.0% |
| !!! | ATATTACTTAATCTAATTTT+TGG | + | chr4.4:67599099-67599118 | MS.gene77688:intron | 10.0% |
| !! | TTTAAAAAATTCAACATTTG+CGG | + | chr4.4:67599399-67599418 | MS.gene77688:intron | 15.0% |
| !!! | AAAAAAAAAAATGGTTTTGA+AGG | - | chr4.4:67599448-67599467 | None:intergenic | 15.0% |
| !!! | AAAAAAAAAATGGTTTTGAA+GGG | - | chr4.4:67599382-67599401 | None:intergenic | 15.0% |
| !!! | TAAAAAAAAAATGGTTTTGA+AGG | - | chr4.4:67599383-67599402 | None:intergenic | 15.0% |
| !! | GTAATATTCCTAAATATTGT+TGG | - | chr4.4:67599086-67599105 | None:intergenic | 20.0% |
| !!! | AAAAAAAAATGGTTTTGAAG+GGG | - | chr4.4:67599381-67599400 | None:intergenic | 20.0% |
| ! | TCTTTCATTTATCTTTCAGA+TGG | + | chr4.4:67598861-67598880 | MS.gene77688:intron | 25.0% |
| ! | TGAAAAACCAATACATTAGT+GGG | - | chr4.4:67599146-67599165 | None:intergenic | 25.0% |
| ! | TTGAAAAACCAATACATTAG+TGG | - | chr4.4:67599147-67599166 | None:intergenic | 25.0% |
| ATGATTTATTGCTTCTTCAG+AGG | + | chr4.4:67598722-67598741 | MS.gene77688:CDS | 30.0% | |
| CAGATGTATCTATTGTAATG+AGG | + | chr4.4:67599043-67599062 | MS.gene77688:intron | 30.0% | |
| TATTCCTAAATATTGTTGGC+CGG | - | chr4.4:67599082-67599101 | None:intergenic | 30.0% | |
| !!! | AGTTTTCACTTGGTTTTGAA+GGG | - | chr4.4:67599511-67599530 | None:intergenic | 30.0% |
| AAAGTTTCACTCTGTTGTTG+AGG | + | chr4.4:67598969-67598988 | MS.gene77688:CDS | 35.0% | |
| AACTTAACATTTGCGACTGT+TGG | + | chr4.4:67599341-67599360 | MS.gene77688:intron | 35.0% | |
| ATGATTGCCCACTAATGTAT+TGG | + | chr4.4:67599136-67599155 | MS.gene77688:intron | 35.0% | |
| ATTTATTGCTTCTTCAGAGG+AGG | + | chr4.4:67598725-67598744 | MS.gene77688:CDS | 35.0% | |
| TCAACAACAGAGTGAAACTT+TGG | - | chr4.4:67598970-67598989 | None:intergenic | 35.0% | |
| TTTATTGCTTCTTCAGAGGA+GGG | + | chr4.4:67598726-67598745 | MS.gene77688:CDS | 35.0% | |
| ! | ATTTTGTCATCTTCAGAGTG+TGG | + | chr4.4:67598805-67598824 | MS.gene77688:CDS | 35.0% |
| ! | GCAAATGAAGAGTTTTCACT+TGG | - | chr4.4:67599521-67599540 | None:intergenic | 35.0% |
| ! | TGAAACTTTGGTGTAAGCAT+AGG | - | chr4.4:67598958-67598977 | None:intergenic | 35.0% |
| ! | TTTTCCGGCCAACAATATTT+AGG | + | chr4.4:67599075-67599094 | MS.gene77688:intron | 35.0% |
| !!! | GAGTTTTCACTTGGTTTTGA+AGG | - | chr4.4:67599512-67599531 | None:intergenic | 35.0% |
| !!! | GTTTTCACTTGGTTTTGAAG+GGG | - | chr4.4:67599510-67599529 | None:intergenic | 35.0% |
| AACATTTGCGACTGTTGTGA+AGG | + | chr4.4:67599476-67599495 | MS.gene77688:intron | 40.0% | |
| AACTCTTCATTTGCGACTGT+TGG | + | chr4.4:67599527-67599546 | MS.gene77688:CDS | 40.0% | |
| AATTCAACATTTGCGGCTGT+TGG | + | chr4.4:67599406-67599425 | MS.gene77688:intron | 40.0% | |
| ACATTTGCGACTGTTGTGAA+GGG | + | chr4.4:67599477-67599496 | MS.gene77688:intron | 40.0% | |
| ACTCTTCATTTGCGACTGTT+GGG | + | chr4.4:67599528-67599547 | MS.gene77688:CDS | 40.0% | |
| ATACTCAGAAGATGATGCCA+AGG | + | chr4.4:67598751-67598770 | MS.gene77688:CDS | 40.0% | |
| CATGATCCATGCACTTCTAT+TGG | - | chr4.4:67599013-67599032 | None:intergenic | 40.0% | |
| TATTTGTACCATGACAGCCT+TGG | - | chr4.4:67598771-67598790 | None:intergenic | 40.0% | |
| ! | ATGAGGCTCTTCAACTTTTC+CGG | + | chr4.4:67599060-67599079 | MS.gene77688:intron | 40.0% |
| ! | TGAAAGAATTTGCTCACCTC+AGG | - | chr4.4:67598848-67598867 | None:intergenic | 40.0% |
| GCACATAGATCTTAAGCCTG+AGG | + | chr4.4:67598829-67598848 | MS.gene77688:CDS | 45.0% | |
| ! | AACTGTCGCAACACCTTTTG+CGG | + | chr4.4:67599551-67599570 | MS.gene77688:CDS | 45.0% |
| ! | ACATTTGCGACTGTTGGTGA+AGG | + | chr4.4:67599347-67599366 | MS.gene77688:intron | 45.0% |
| ! | CATTTGCGACTGTTGGTGAA+GGG | + | chr4.4:67599348-67599367 | MS.gene77688:intron | 45.0% |
| !! | AAAAATAAAATAAAATTGAA+TGG | - | chr4.4:67599314-67599333 | None:intergenic | 5.0% |
| !! | AAAATAAAATAAAATTGAAT+GGG | - | chr4.4:67599313-67599332 | None:intergenic | 5.0% |
| !!! | TGAATTTTTTAAAAAAAAAA+TGG | - | chr4.4:67599392-67599411 | None:intergenic | 5.0% |
| ACCCCAACAAACGTGACTGT+TGG | + | chr4.4:67599619-67599638 | MS.gene77688:CDS | 50.0% | |
| AGATGATGCCAAGGCTGTCA+TGG | + | chr4.4:67598760-67598779 | MS.gene77688:CDS | 50.0% | |
| CCCAACAAACGTGACTGTTG+GGG | + | chr4.4:67599621-67599640 | MS.gene77688:CDS | 50.0% | |
| CCCCAACAAACGTGACTGTT+GGG | + | chr4.4:67599620-67599639 | MS.gene77688:CDS | 50.0% | |
| CCCCAACAGTCACGTTTGTT+GGG | - | chr4.4:67599624-67599643 | None:intergenic | 50.0% | |
| GTTGAGGATAGCAGCAGATG+CGG | + | chr4.4:67598985-67599004 | MS.gene77688:CDS | 50.0% | |
| TCCCCAACAGTCACGTTTGT+TGG | - | chr4.4:67599625-67599644 | None:intergenic | 50.0% | |
| ! | ACATTTGCGGCTGTTGGTGA+AGG | + | chr4.4:67599412-67599431 | MS.gene77688:intron | 50.0% |
| ! | CATTTGCGGCTGTTGGTGAA+GGG | + | chr4.4:67599413-67599432 | MS.gene77688:intron | 50.0% |
| ! | CCCAACAGTCACGTTTGTTG+GGG | - | chr4.4:67599623-67599642 | None:intergenic | 50.0% |
| ! | GCGGTACCAATAGAAGTGCA+TGG | + | chr4.4:67599004-67599023 | MS.gene77688:CDS | 50.0% |
| ! | ACCTTTTGCGGCAGTTGGAG+CGG | + | chr4.4:67599563-67599582 | MS.gene77688:CDS | 55.0% |
| ! | CTGTCTTTTGCGACCCTTCG+TGG | - | chr4.4:67599594-67599613 | None:intergenic | 55.0% |
| ! | GCAACACCTTTTGCGGCAGT+TGG | + | chr4.4:67599558-67599577 | MS.gene77688:CDS | 55.0% |
| TGGAGCGGCTGTTCCACGAA+GGG | + | chr4.4:67599578-67599597 | MS.gene77688:CDS | 60.0% | |
| TTGGAGCGGCTGTTCCACGA+AGG | + | chr4.4:67599577-67599596 | MS.gene77688:CDS | 60.0% | |
| GCCGCTCCAACTGCCGCAAA+AGG | - | chr4.4:67599567-67599586 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 67598722 | 67599677 | 67598722 | ID=MS.gene77688 |
| chr4.4 | mRNA | 67598722 | 67599677 | 67598722 | ID=MS.gene77688.t1;Parent=MS.gene77688 |
| chr4.4 | exon | 67598722 | 67598850 | 67598722 | ID=MS.gene77688.t1.exon1;Parent=MS.gene77688.t1 |
| chr4.4 | CDS | 67598722 | 67598850 | 67598722 | ID=cds.MS.gene77688.t1;Parent=MS.gene77688.t1 |
| chr4.4 | exon | 67598931 | 67599056 | 67598931 | ID=MS.gene77688.t1.exon2;Parent=MS.gene77688.t1 |
| chr4.4 | CDS | 67598931 | 67599056 | 67598931 | ID=cds.MS.gene77688.t1;Parent=MS.gene77688.t1 |
| chr4.4 | exon | 67599498 | 67599677 | 67599498 | ID=MS.gene77688.t1.exon3;Parent=MS.gene77688.t1 |
| chr4.4 | CDS | 67599498 | 67599677 | 67599498 | ID=cds.MS.gene77688.t1;Parent=MS.gene77688.t1 |
| Gene Sequence |
| Protein sequence |
