AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77726

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77726.t1 MTR_1g047060 51.064 94 39 2 109 200 1 89 3.76e-23 89.4
MS.gene77726.t1 MTR_7g105170 29.885 174 98 7 1 152 1 172 4.16e-17 77.4
MS.gene77726.t1 MTR_6g477900 32.386 176 89 8 4 165 6 165 1.98e-16 75.5
MS.gene77726.t1 MTR_1g096430 30.769 156 92 5 4 148 7 157 6.30e-16 75.1
MS.gene77726.t1 MTR_5g012080 31.210 157 86 6 4 145 7 156 1.88e-15 73.9
MS.gene77726.t1 MTR_2g062730 30.769 156 87 5 4 145 10 158 3.32e-15 73.2
MS.gene77726.t1 MTR_8g023840 32.903 155 82 8 4 145 11 156 3.73e-15 73.6
MS.gene77726.t1 MTR_2g086880 31.081 148 83 6 6 144 20 157 4.47e-15 73.6
MS.gene77726.t1 MTR_2g086880 31.081 148 83 6 6 144 20 157 5.12e-15 73.2
MS.gene77726.t1 MTR_1g008740 33.758 157 85 7 2 145 25 175 6.37e-15 72.4
MS.gene77726.t1 MTR_7g100990 33.540 161 78 9 4 144 11 162 6.67e-15 72.4
MS.gene77726.t1 MTR_8g023900 33.333 156 80 8 4 144 12 158 6.76e-15 72.8
MS.gene77726.t1 MTR_8g023900 31.333 150 83 7 7 144 196 337 1.25e-11 63.2
MS.gene77726.t1 MTR_1g008740 33.758 157 85 7 2 145 11 161 8.87e-15 72.0
MS.gene77726.t1 MTR_4g101680 32.051 156 84 6 4 145 7 154 1.54e-14 71.6
MS.gene77726.t1 MTR_3g096140 32.704 159 84 7 2 145 5 155 2.21e-14 70.5
MS.gene77726.t1 MTR_8g023860 30.573 157 89 7 1 145 9 157 4.83e-14 70.1
MS.gene77726.t1 MTR_3g096140 32.704 159 84 7 2 145 5 155 5.43e-14 70.1
MS.gene77726.t1 MTR_1g069805 33.766 154 81 9 4 145 10 154 1.24e-13 68.2
MS.gene77726.t1 MTR_6g011860 29.268 164 87 6 4 145 15 171 5.38e-13 67.0
MS.gene77726.t1 MTR_7g097090 34.416 154 80 9 4 145 10 154 5.50e-13 66.6
MS.gene77726.t1 MTR_8g093790 29.240 171 100 6 4 171 10 162 1.19e-12 64.3
MS.gene77726.t1 MTR_8g094580 28.750 160 88 6 4 146 7 157 2.32e-12 64.7
MS.gene77726.t1 MTR_8g023930 31.373 153 89 6 2 144 22 168 2.37e-12 65.5
MS.gene77726.t1 MTR_4g098630 34.559 136 72 8 4 129 6 134 2.54e-12 65.1
MS.gene77726.t1 MTR_5g069030 32.500 160 79 10 4 145 25 173 2.79e-12 65.5
MS.gene77726.t1 MTR_8g059170 30.380 158 85 7 4 145 14 162 6.95e-12 63.5
MS.gene77726.t1 MTR_8g467490 28.743 167 89 8 2 145 12 171 8.45e-12 63.5
MS.gene77726.t1 MTR_2g079990 28.177 181 99 8 4 162 16 187 8.61e-12 63.5
MS.gene77726.t1 MTR_7g085220 28.743 167 87 7 4 145 8 167 1.13e-11 63.2
MS.gene77726.t1 MTR_1g090720 32.099 162 83 7 4 145 42 196 1.52e-11 63.2
MS.gene77726.t1 MTR_4g075980 29.333 150 89 6 6 145 17 159 1.64e-11 62.4
MS.gene77726.t1 MTR_3g088110 28.931 159 88 6 4 146 7 156 1.78e-11 62.4
MS.gene77726.t1 MTR_6g084430 30.968 155 88 7 2 145 3 149 1.99e-11 62.4
MS.gene77726.t1 MTR_7g005280 30.968 155 83 7 4 145 15 158 2.35e-11 61.6
MS.gene77726.t1 MTR_4g108760 31.210 157 85 8 4 145 17 165 2.80e-11 62.0
MS.gene77726.t1 MTR_4g052620 30.061 163 90 7 2 145 3 160 3.56e-11 61.2
MS.gene77726.t1 MTR_2g086690 27.329 161 92 7 5 145 5 160 5.40e-11 60.5
MS.gene77726.t1 MTR_8g093580 30.769 156 87 7 4 145 6 154 5.89e-11 61.2
MS.gene77726.t1 MTR_4g094302 32.283 127 76 5 4 129 11 128 9.95e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77726.t1 AT2G27300 31.724 145 90 4 4 144 14 153 9.33e-18 80.5
MS.gene77726.t1 AT4G36160 33.117 154 85 6 4 145 14 161 2.95e-16 76.6
MS.gene77726.t1 AT4G36160 33.117 154 85 6 4 145 10 157 3.01e-16 76.6
MS.gene77726.t1 AT4G36160 33.117 154 85 6 4 145 10 157 3.01e-16 76.6
MS.gene77726.t1 AT2G24430 34.395 157 83 7 2 145 14 163 4.32e-16 75.5
MS.gene77726.t1 AT2G24430 34.395 157 83 7 2 145 14 163 4.32e-16 75.5
MS.gene77726.t1 AT1G71930 31.410 156 91 5 4 148 9 159 5.74e-16 75.5
MS.gene77726.t1 AT1G71930 31.410 156 91 5 4 148 9 159 5.74e-16 75.5
MS.gene77726.t1 AT3G44290 31.081 148 83 6 6 144 16 153 6.24e-16 73.9
MS.gene77726.t1 AT1G62700 32.692 156 83 7 4 145 7 154 2.25e-15 73.9
MS.gene77726.t1 AT1G62700 32.692 156 83 7 4 145 7 154 2.25e-15 73.9
MS.gene77726.t1 AT3G44290 29.655 145 89 5 6 144 16 153 4.01e-15 73.2
MS.gene77726.t1 AT4G17980 32.278 158 84 7 4 144 6 157 9.08e-15 71.6
MS.gene77726.t1 AT4G17980 32.278 158 84 7 4 144 6 157 9.75e-15 71.2
MS.gene77726.t1 AT5G22290 30.822 146 87 6 6 144 23 161 2.16e-14 70.9
MS.gene77726.t1 AT1G61110 34.848 132 73 4 4 129 16 140 2.38e-14 70.9
MS.gene77726.t1 AT1G65910 33.121 157 82 8 4 145 6 154 2.93e-14 71.2
MS.gene77726.t1 AT1G54330 30.921 152 89 6 4 145 6 151 3.67e-14 70.1
MS.gene77726.t1 AT1G32510 31.515 165 82 8 3 144 5 161 3.72e-14 69.7
MS.gene77726.t1 AT4G35580 30.247 162 92 7 1 148 6 160 4.00e-14 70.9
MS.gene77726.t1 AT4G35580 30.247 162 92 7 1 148 6 160 4.07e-14 70.9
MS.gene77726.t1 AT4G35580 29.944 177 101 8 1 161 6 175 4.33e-14 70.5
MS.gene77726.t1 AT5G46590 32.331 133 77 5 4 129 6 132 4.98e-14 69.3
MS.gene77726.t1 AT1G26870 32.903 155 88 6 2 148 14 160 6.06e-14 70.1
MS.gene77726.t1 AT5G24590 30.573 157 87 5 4 145 13 162 1.85e-13 68.6
MS.gene77726.t1 AT3G18400 31.847 157 87 8 2 146 3 151 2.16e-13 68.2
MS.gene77726.t1 AT4G27410 29.814 161 90 7 4 150 14 165 2.19e-13 67.8
MS.gene77726.t1 AT1G69490 31.250 176 96 8 4 162 9 176 2.25e-13 67.4
MS.gene77726.t1 AT1G76420 30.818 159 84 7 4 146 22 170 4.73e-13 67.0
MS.gene77726.t1 AT5G39610 31.210 157 85 7 4 145 20 168 6.77e-13 66.2
MS.gene77726.t1 AT2G43000 32.468 154 82 8 6 145 20 165 1.17e-12 65.5
MS.gene77726.t1 AT5G39820 33.835 133 70 6 6 129 22 145 1.26e-12 65.9
MS.gene77726.t1 AT1G52890 30.769 156 85 7 4 145 14 160 1.50e-12 65.5
MS.gene77726.t1 AT3G15500 30.323 155 87 7 4 145 14 160 1.86e-12 65.5
MS.gene77726.t1 AT5G04410 30.968 155 88 6 4 145 9 157 1.92e-12 65.9
MS.gene77726.t1 AT5G09330 30.968 155 88 6 4 145 6 154 2.85e-12 65.5
MS.gene77726.t1 AT5G09330 30.968 155 88 6 4 145 6 154 2.85e-12 65.5
MS.gene77726.t1 AT5G09330 30.968 155 88 6 4 145 6 154 2.85e-12 65.5
MS.gene77726.t1 AT5G09330 30.968 155 88 6 4 145 6 154 2.85e-12 65.5
MS.gene77726.t1 AT5G09330 30.968 155 88 6 4 145 6 154 2.85e-12 65.5
MS.gene77726.t1 AT3G29035 32.576 132 75 5 4 129 24 147 4.44e-12 64.3
MS.gene77726.t1 AT1G34190 29.936 157 91 6 2 145 14 164 1.01e-11 63.5
MS.gene77726.t1 AT2G33480 32.051 156 80 7 4 145 15 158 1.12e-11 62.8
MS.gene77726.t1 AT5G61430 30.247 162 90 7 4 150 16 169 1.88e-11 62.4
MS.gene77726.t1 AT4G27410 27.429 175 93 7 4 150 14 182 2.28e-11 62.4
MS.gene77726.t1 AT1G32870 32.061 131 79 5 4 130 10 134 4.22e-11 62.0
MS.gene77726.t1 AT3G04060 28.342 187 110 7 4 174 20 198 4.28e-11 61.6
MS.gene77726.t1 AT1G32870 32.061 131 79 5 4 130 10 134 4.41e-11 62.0
MS.gene77726.t1 AT5G64530 28.351 194 104 7 4 175 3 183 4.70e-11 59.7
MS.gene77726.t1 AT1G32870 32.061 131 79 5 4 130 46 170 6.91e-11 61.2
MS.gene77726.t1 AT5G18270 30.380 158 86 7 4 145 21 170 8.44e-11 60.8
MS.gene77726.t1 AT5G18270 30.380 158 86 7 4 145 21 170 8.53e-11 60.8
MS.gene77726.t1 AT2G17040 29.747 158 78 8 6 145 8 150 8.67e-11 60.5
MS.gene77726.t1 AT5G13180 31.169 154 85 7 4 145 14 158 9.23e-11 60.1

Find 46 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATCTGGACTCTGAATT+TGG 0.253239 4.4:-68517145 MS.gene77726:CDS
CAATGGTTCTGACCTGATTA+AGG 0.258535 4.4:-68517604 MS.gene77726:CDS
CAGAGGAGGCATTGTAAGAT+TGG 0.351545 4.4:-68517477 MS.gene77726:CDS
GGCATTGTAAGATTGGGTTT+TGG 0.353732 4.4:-68517470 MS.gene77726:CDS
TGAAGGAACTCAGCAACTTA+AGG 0.387687 4.4:-68517062 MS.gene77726:CDS
ATAAGTTCAGCTCCTTAATC+AGG 0.404146 4.4:+68517592 None:intergenic
GAAGAGGTTCAGAATTTCAA+TGG 0.424440 4.4:-68517621 MS.gene77726:CDS
GAAGGAACTCAGCAACTTAA+GGG 0.437746 4.4:-68517061 MS.gene77726:CDS
CCTCCTCTGCTCATCAACTT+TGG 0.447718 4.4:+68517491 None:intergenic
AAAACGCGGCAAACAACAAA+AGG 0.449123 4.4:+68517184 None:intergenic
AGAGGAGGCATTGTAAGATT+GGG 0.450614 4.4:-68517476 MS.gene77726:CDS
TGGAGTTGATAACGTGGTTG+TGG 0.453423 4.4:-68517418 MS.gene77726:CDS
GAATTCCTTCCACCTGGTGT+TGG 0.454396 4.4:-68517699 MS.gene77726:CDS
CTGCCCTAGAGGGATGGATC+TGG 0.463977 4.4:-68517158 MS.gene77726:CDS
TTCGATGACAAGGCGAATGA+AGG 0.469350 4.4:-68517079 MS.gene77726:CDS
GCAATTCTAACGGATCATAC+TGG 0.483561 4.4:+68517568 None:intergenic
GAGTCCAGATCCATCCCTCT+AGG 0.508074 4.4:+68517154 None:intergenic
GTGAAGCAGTAAAATTCCCT+TGG 0.511128 4.4:+68517516 None:intergenic
ACCTTCATCGACATCAGAAA+AGG 0.512248 4.4:+68517120 None:intergenic
GGAGTTGATAACGTGGTTGT+GGG 0.517524 4.4:-68517417 MS.gene77726:CDS
GGAGTTGTTCGAGACATTTG+TGG 0.527780 4.4:-68517438 MS.gene77726:CDS
GGCTACTCAAGCCACTCCTT+AGG 0.532323 4.4:-68516990 None:intergenic
AGTAAAATTCCCTTGGGTGA+CGG 0.539767 4.4:+68517523 None:intergenic
TAACAAGGAGCTCATCTGAA+GGG 0.545729 4.4:+68517670 None:intergenic
AGAACGCGATCAAAACTAGC+TGG 0.546737 4.4:-68517353 MS.gene77726:CDS
AGGAGTGGCTTGAGTAGCCA+TGG 0.558328 4.4:+68516994 None:intergenic
TGAAGCAGTAAAATTCCCTT+GGG 0.559532 4.4:+68517517 None:intergenic
GAAGGGGCGAAGCCAACACC+AGG 0.562826 4.4:+68517687 None:intergenic
CGAAGCCAACACCAGGTGGA+AGG 0.569669 4.4:+68517694 None:intergenic
AGGCTATGCATCGCATTCCA+TGG 0.571087 4.4:-68517011 MS.gene77726:CDS
CCAAAGTTGATGAGCAGAGG+AGG 0.574327 4.4:-68517491 MS.gene77726:CDS
AGTCCAGATCCATCCCTCTA+GGG 0.585447 4.4:+68517155 None:intergenic
CACTCAGGTTAACAACCCTC+AGG 0.585811 4.4:-68517316 MS.gene77726:intron
CTAACAAGGAGCTCATCTGA+AGG 0.597005 4.4:+68517669 None:intergenic
GCAATGGAATTCCTTCCACC+TGG 0.603855 4.4:-68517705 MS.gene77726:CDS
AAGGAACTCAGCAACTTAAG+GGG 0.605135 4.4:-68517060 MS.gene77726:CDS
GGACTAAGAGGCAATTCTAA+CGG 0.613861 4.4:+68517558 None:intergenic
TGGCAAGGTAGTAGCTAACA+AGG 0.615826 4.4:+68517655 None:intergenic
AACAAGGAGCTCATCTGAAG+GGG 0.618835 4.4:+68517671 None:intergenic
CTGCCAAAGTTGATGAGCAG+AGG 0.624165 4.4:-68517494 MS.gene77726:CDS
TGATGACGACTTCGATGACA+AGG 0.631139 4.4:-68517089 MS.gene77726:CDS
GGGGCGAAGCCAACACCAGG+TGG 0.632420 4.4:+68517690 None:intergenic
GACTATGTACGAGTACACTC+AGG 0.643748 4.4:-68517331 MS.gene77726:CDS
GCTAGTTCTAGTGCTGAGCA+AGG 0.673401 4.4:-68517031 MS.gene77726:CDS
AGGAACTCAGCAACTTAAGG+GGG 0.676579 4.4:-68517059 MS.gene77726:CDS
CATTTGTGGAGTTGATAACG+TGG 0.688917 4.4:-68517424 MS.gene77726:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATATAAATAATTATGC+AGG - chr4.4:68517484-68517503 MS.gene77726:CDS 10.0%
!! TCGATGATTAATTTATGTAT+TGG - chr4.4:68517444-68517463 MS.gene77726:CDS 20.0%
!!! TCTCTTTTTATGTTCTATAT+AGG - chr4.4:68517306-68517325 MS.gene77726:intron 20.0%
! TCGATGAAATAATGAACATA+TGG + chr4.4:68517428-68517447 None:intergenic 25.0%
!! AAGAAAAATGATGGACTAAG+AGG + chr4.4:68517150-68517169 None:intergenic 30.0%
!!! AACCTCTTCATTGTTTTTGT+TGG + chr4.4:68517061-68517080 None:intergenic 30.0%
ATAAGTTCAGCTCCTTAATC+AGG + chr4.4:68517104-68517123 None:intergenic 35.0%
GAAGAGGTTCAGAATTTCAA+TGG - chr4.4:68517072-68517091 MS.gene77726:CDS 35.0%
TGAAGCAGTAAAATTCCCTT+GGG + chr4.4:68517179-68517198 None:intergenic 35.0%
TGCCAACAAAAACAATGAAG+AGG - chr4.4:68517056-68517075 MS.gene77726:CDS 35.0%
!!! CTTCATTGTTTTTGTTGGCA+AGG + chr4.4:68517056-68517075 None:intergenic 35.0%
AAAACGCGGCAAACAACAAA+AGG + chr4.4:68517512-68517531 None:intergenic 40.0%
AAGGAACTCAGCAACTTAAG+GGG - chr4.4:68517633-68517652 MS.gene77726:CDS 40.0%
ACCTTCATCGACATCAGAAA+AGG + chr4.4:68517576-68517595 None:intergenic 40.0%
AGTAAAATTCCCTTGGGTGA+CGG + chr4.4:68517173-68517192 None:intergenic 40.0%
ATGGATCTGGACTCTGAATT+TGG - chr4.4:68517548-68517567 MS.gene77726:CDS 40.0%
GAAGGAACTCAGCAACTTAA+GGG - chr4.4:68517632-68517651 MS.gene77726:CDS 40.0%
GGACTAAGAGGCAATTCTAA+CGG + chr4.4:68517138-68517157 None:intergenic 40.0%
GTGAAGCAGTAAAATTCCCT+TGG + chr4.4:68517180-68517199 None:intergenic 40.0%
TAACAAGGAGCTCATCTGAA+GGG + chr4.4:68517026-68517045 None:intergenic 40.0%
TGAAGGAACTCAGCAACTTA+AGG - chr4.4:68517631-68517650 MS.gene77726:CDS 40.0%
TTCCCAAATGACATACCTGA+GGG + chr4.4:68517395-68517414 None:intergenic 40.0%
TTTCCCAAATGACATACCTG+AGG + chr4.4:68517396-68517415 None:intergenic 40.0%
! AACCCTCAGGTATGTCATTT+GGG - chr4.4:68517390-68517409 MS.gene77726:CDS 40.0%
! ATTTTTCTTCCGTCACCCAA+GGG - chr4.4:68517161-68517180 MS.gene77726:CDS 40.0%
! GCAATTCTAACGGATCATAC+TGG + chr4.4:68517128-68517147 None:intergenic 40.0%
! GGCATTGTAAGATTGGGTTT+TGG - chr4.4:68517223-68517242 MS.gene77726:intron 40.0%
!! AGAGGAGGCATTGTAAGATT+GGG - chr4.4:68517217-68517236 MS.gene77726:intron 40.0%
!! CAATGGTTCTGACCTGATTA+AGG - chr4.4:68517089-68517108 MS.gene77726:CDS 40.0%
!! CATTTGTGGAGTTGATAACG+TGG - chr4.4:68517269-68517288 MS.gene77726:intron 40.0%
!!! ATTGGGTTTTGGATGCGTAA+GGG - chr4.4:68517234-68517253 MS.gene77726:intron 40.0%
!!! TAGTTTTGATCGCGTTCTGA+GGG + chr4.4:68517338-68517357 None:intergenic 40.0%
AACAAGGAGCTCATCTGAAG+GGG + chr4.4:68517025-68517044 None:intergenic 45.0%
AGAACGCGATCAAAACTAGC+TGG - chr4.4:68517340-68517359 MS.gene77726:CDS 45.0%
AGGAACTCAGCAACTTAAGG+GGG - chr4.4:68517634-68517653 MS.gene77726:CDS 45.0%
CAACCCTCAGGTATGTCATT+TGG - chr4.4:68517389-68517408 MS.gene77726:CDS 45.0%
CTAACAAGGAGCTCATCTGA+AGG + chr4.4:68517027-68517046 None:intergenic 45.0%
GACTATGTACGAGTACACTC+AGG - chr4.4:68517362-68517381 MS.gene77726:CDS 45.0%
GGAGTTGTTCGAGACATTTG+TGG - chr4.4:68517255-68517274 MS.gene77726:intron 45.0%
TGGCAAGGTAGTAGCTAACA+AGG + chr4.4:68517041-68517060 None:intergenic 45.0%
TTCGATGACAAGGCGAATGA+AGG - chr4.4:68517614-68517633 MS.gene77726:CDS 45.0%
! CATTTTTCTTCCGTCACCCA+AGG - chr4.4:68517160-68517179 MS.gene77726:CDS 45.0%
! GCCTTTTCTGATGTCGATGA+AGG - chr4.4:68517572-68517591 MS.gene77726:CDS 45.0%
! TGATGACGACTTCGATGACA+AGG - chr4.4:68517604-68517623 MS.gene77726:CDS 45.0%
!! AGTTTTGATCGCGTTCTGAG+GGG + chr4.4:68517337-68517356 None:intergenic 45.0%
!! CAGAGGAGGCATTGTAAGAT+TGG - chr4.4:68517216-68517235 MS.gene77726:intron 45.0%
!! GGAGTTGATAACGTGGTTGT+GGG - chr4.4:68517276-68517295 MS.gene77726:intron 45.0%
!! GGGTGACGGAAGAAAAATGA+TGG + chr4.4:68517159-68517178 None:intergenic 45.0%
!! TGGAGTTGATAACGTGGTTG+TGG - chr4.4:68517275-68517294 MS.gene77726:intron 45.0%
!!! CTAGTTTTGATCGCGTTCTG+AGG + chr4.4:68517339-68517358 None:intergenic 45.0%
!!! GATTGGGTTTTGGATGCGTA+AGG - chr4.4:68517233-68517252 MS.gene77726:intron 45.0%
AGGCTATGCATCGCATTCCA+TGG - chr4.4:68517682-68517701 MS.gene77726:CDS 50.0%
AGTCCAGATCCATCCCTCTA+GGG + chr4.4:68517541-68517560 None:intergenic 50.0%
CACTCAGGTTAACAACCCTC+AGG - chr4.4:68517377-68517396 MS.gene77726:CDS 50.0%
CCTCCTCTGCTCATCAACTT+TGG + chr4.4:68517205-68517224 None:intergenic 50.0%
GAATTCCTTCCACCTGGTGT+TGG - chr4.4:68516994-68517013 MS.gene77726:CDS 50.0%
!! CCAAAGTTGATGAGCAGAGG+AGG - chr4.4:68517202-68517221 MS.gene77726:intron 50.0%
!! CTCTAGGGCAGTCAAAAACG+CGG + chr4.4:68517526-68517545 None:intergenic 50.0%
!! CTGCCAAAGTTGATGAGCAG+AGG - chr4.4:68517199-68517218 MS.gene77726:intron 50.0%
!! GCGTTTTTGACTGCCCTAGA+GGG - chr4.4:68517525-68517544 MS.gene77726:CDS 50.0%
!! GCTAGTTCTAGTGCTGAGCA+AGG - chr4.4:68517662-68517681 MS.gene77726:CDS 50.0%
!! TTTTGACTGCCCTAGAGGGA+TGG - chr4.4:68517529-68517548 MS.gene77726:CDS 50.0%
ACTCAGCAACTTAAGGGGGC+TGG - chr4.4:68517638-68517657 MS.gene77726:CDS 55.0%
AGGAGTGGCTTGAGTAGCCA+TGG + chr4.4:68517702-68517721 None:intergenic 55.0%
CTCAGCAACTTAAGGGGGCT+GGG - chr4.4:68517639-68517658 MS.gene77726:CDS 55.0%
GAGTCCAGATCCATCCCTCT+AGG + chr4.4:68517542-68517561 None:intergenic 55.0%
TCAGCAACTTAAGGGGGCTG+GGG - chr4.4:68517640-68517659 MS.gene77726:CDS 55.0%
!! CGCGTTTTTGACTGCCCTAG+AGG - chr4.4:68517524-68517543 MS.gene77726:CDS 55.0%
CGAAGCCAACACCAGGTGGA+AGG + chr4.4:68517002-68517021 None:intergenic 60.0%
CTGCCCTAGAGGGATGGATC+TGG - chr4.4:68517535-68517554 MS.gene77726:CDS 60.0%
GAAGGGGCGAAGCCAACACC+AGG + chr4.4:68517009-68517028 None:intergenic 65.0%
GGGGCGAAGCCAACACCAGG+TGG + chr4.4:68517006-68517025 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 68516991 68517724 68516991 ID=MS.gene77726
chr4.4 mRNA 68516991 68517724 68516991 ID=MS.gene77726.t1;Parent=MS.gene77726
chr4.4 exon 68517317 68517724 68517317 ID=MS.gene77726.t1.exon1;Parent=MS.gene77726.t1
chr4.4 CDS 68517317 68517724 68517317 ID=cds.MS.gene77726.t1;Parent=MS.gene77726.t1
chr4.4 exon 68516991 68517209 68516991 ID=MS.gene77726.t1.exon2;Parent=MS.gene77726.t1
chr4.4 CDS 68516991 68517209 68516991 ID=cds.MS.gene77726.t1;Parent=MS.gene77726.t1
Gene Sequence

>MS.gene77726

ATGGAATTCCTTCCACCTGGTGTTGGCTTCGCCCCTTCAGATGAGCTCCTTGTTAGCTACTACCTTGCCAACAAAAACAATGAAGAGGTTCAGAATTTCAATGGTTCTGACCTGATTAAGGAGCTGAACTTATACCAGTATGATCCGTTAGAATTGCCTCTTAGTCCATCATTTTTCTTCCGTCACCCAAGGGAATTTTACTGCTTCACTGCCAAAGTTGATGAGCAGAGGAGGCATTGTAAGATTGGGTTTTGGATGCGTAAGGGAGTTGTTCGAGACATTTGTGGAGTTGATAACGTGGTTGTGGGAAAGAAGTCTCTTTTTATGTTCTATATAGGAAATTCCCCTCAGAACGCGATCAAAACTAGCTGGACTATGTACGAGTACACTCAGGTTAACAACCCTCAGGTATGTCATTTGGGAAAACTAAGTTTCCATATGTTCATTATTTCATCGATGATTAATTTATGTATTGGTAACGACAAACTTTTTTTTTAATATAAATAATTATGCAGGCTCCTTTTGTTGTTTGCCGCGTTTTTGACTGCCCTAGAGGGATGGATCTGGACTCTGAATTTGGAGCCTTTTCTGATGTCGATGAAGGTGATGCTGCTGATGACGACTTCGATGACAAGGCGAATGAAGGAACTCAGCAACTTAAGGGGGCTGGGGCTAGTTCTAGTGCTGAGCAAGGCTATGCATCGCATTCCATGGCTACTCAAGCCACTCCTTAG
Protein sequence

>MS.gene77726.t1

MEFLPPGVGFAPSDELLVSYYLANKNNEEVQNFNGSDLIKELNLYQYDPLELPLSPSFFFRHPREFYCFTAKVDEQRRHCKIGFWMRKGVVRDICGVDNVVVGKKSLFMFYIGNSPQNAIKTSWTMYEYTQVNNPQAPFVVCRVFDCPRGMDLDSEFGAFSDVDEGDAADDDFDDKANEGTQQLKGAGASSSAEQGYASHSMATQATP