AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77982

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77982.t1 MTR_3g022830 96.552 435 15 0 9 443 4 438 0.0 875
MS.gene77982.t1 MTR_5g009080 54.566 449 192 5 7 443 32 480 8.82e-169 483
MS.gene77982.t1 MTR_4g076140 49.888 447 214 4 7 443 25 471 7.30e-159 457
MS.gene77982.t1 MTR_3g022580 72.251 191 22 2 253 443 26 185 8.55e-93 278
MS.gene77982.t1 MTR_5g097480 30.788 419 230 6 31 441 178 544 5.68e-61 207
MS.gene77982.t1 MTR_1g029420 29.976 417 236 4 31 441 226 592 3.45e-59 204
MS.gene77982.t1 MTR_3g022005 91.667 96 8 0 348 443 32 127 7.64e-59 189
MS.gene77982.t1 MTR_2g082090 30.935 417 230 7 31 440 213 578 1.16e-55 194
MS.gene77982.t1 MTR_3g027430 53.211 218 41 7 13 230 124 280 2.77e-55 187
MS.gene77982.t1 MTR_3g027430 56.818 88 4 1 356 443 279 332 1.13e-22 98.6
MS.gene77982.t1 MTR_5g094450 27.685 419 247 6 31 441 162 532 5.96e-55 191
MS.gene77982.t1 MTR_3g089055 28.302 424 248 4 24 441 192 565 1.16e-54 191
MS.gene77982.t1 MTR_3g089055 28.302 424 248 4 24 441 197 570 1.83e-54 191
MS.gene77982.t1 MTR_7g027190 29.358 436 220 8 31 441 301 673 8.39e-54 191
MS.gene77982.t1 MTR_3g056110 27.818 417 245 6 31 441 176 542 1.19e-52 185
MS.gene77982.t1 MTR_7g074650 29.398 415 224 8 33 441 441 792 1.24e-52 189
MS.gene77982.t1 MTR_4g133660 26.841 421 243 7 31 440 176 542 4.22e-47 170
MS.gene77982.t1 MTR_4g102790 27.316 421 243 8 31 440 293 661 1.44e-46 171
MS.gene77982.t1 MTR_3g021320 58.000 150 35 3 130 276 1 125 3.48e-46 156
MS.gene77982.t1 MTR_8g442410 28.169 426 235 9 31 441 156 525 7.80e-45 164
MS.gene77982.t1 MTR_2g097467 28.469 418 237 8 31 440 290 653 1.88e-44 164
MS.gene77982.t1 MTR_4g064160 27.990 418 242 7 31 440 317 683 2.44e-44 164
MS.gene77982.t1 MTR_2g097410 26.555 418 250 6 31 440 372 740 2.54e-44 165
MS.gene77982.t1 MTR_2g026250 26.027 438 258 8 19 441 210 596 6.49e-44 162
MS.gene77982.t1 MTR_4g064200 28.708 418 237 8 31 440 285 649 6.18e-43 160
MS.gene77982.t1 MTR_7g062120 27.273 418 247 6 31 441 373 740 5.11e-42 158
MS.gene77982.t1 MTR_4g064150 27.423 423 242 9 31 440 362 732 6.14e-42 158
MS.gene77982.t1 MTR_2g097350 26.966 445 260 10 6 440 250 639 1.01e-41 157
MS.gene77982.t1 MTR_2g097310 26.969 419 243 8 31 440 273 637 2.55e-41 155
MS.gene77982.t1 MTR_2g097473 27.818 417 241 7 31 440 290 653 2.93e-41 155
MS.gene77982.t1 MTR_2g097390 25.542 415 254 7 31 440 276 640 5.75e-41 154
MS.gene77982.t1 MTR_4g104020 26.005 423 249 7 31 441 150 520 1.22e-40 152
MS.gene77982.t1 MTR_3g025350 43.396 212 36 5 181 391 1 129 1.47e-40 141
MS.gene77982.t1 MTR_4g064120 26.316 418 245 9 31 436 255 621 5.76e-40 152
MS.gene77982.t1 MTR_4g064180 26.139 417 247 8 31 436 255 621 2.98e-39 149
MS.gene77982.t1 MTR_2g097463 27.338 417 243 7 31 440 291 654 1.83e-38 147
MS.gene77982.t1 MTR_2g034280 28.271 428 236 9 31 442 204 576 1.46e-36 141
MS.gene77982.t1 MTR_4g097080 23.820 445 271 9 10 441 114 503 5.27e-36 139
MS.gene77982.t1 MTR_4g076020 28.641 412 222 12 33 441 87 429 7.83e-36 137
MS.gene77982.t1 MTR_2g034250 27.739 429 240 8 31 442 211 586 9.04e-36 139
MS.gene77982.t1 MTR_4g077760 27.103 428 240 10 26 441 188 555 9.81e-36 139
MS.gene77982.t1 MTR_1g069725 26.056 426 248 10 23 443 102 465 1.29e-35 137
MS.gene77982.t1 MTR_2g034260 27.442 430 239 9 31 442 211 585 3.18e-34 135
MS.gene77982.t1 MTR_2g097380 24.939 409 227 7 35 440 229 560 3.78e-34 134
MS.gene77982.t1 MTR_2g089100 25.943 424 266 10 31 440 53 442 5.31e-33 130
MS.gene77982.t1 MTR_5g015490 23.349 424 264 8 31 441 115 490 6.96e-32 127
MS.gene77982.t1 MTR_1g106590 50.000 110 55 0 32 141 132 241 7.11e-30 119
MS.gene77982.t1 MTR_1g106590 48.936 94 45 2 256 346 247 340 1.83e-14 74.7
MS.gene77982.t1 MTR_5g058860 24.190 401 236 7 56 440 148 496 8.17e-29 119
MS.gene77982.t1 MTR_3g065980 39.752 161 83 3 296 442 381 541 2.53e-28 117
MS.gene77982.t1 MTR_3g065980 31.100 209 130 4 31 234 173 372 8.66e-28 116
MS.gene77982.t1 MTR_3g072710 24.215 446 256 13 21 440 110 499 6.79e-28 116
MS.gene77982.t1 MTR_1g096030 24.070 457 258 15 15 441 62 459 2.69e-27 114
MS.gene77982.t1 MTR_4g095500 23.982 442 270 10 31 440 60 467 6.51e-26 110
MS.gene77982.t1 MTR_7g109580 23.737 396 236 9 57 442 216 555 1.62e-24 106
MS.gene77982.t1 MTR_7g057230 26.190 420 242 13 31 440 295 656 2.26e-24 106
MS.gene77982.t1 MTR_5g015950 22.864 433 288 11 31 440 36 445 1.20e-23 103
MS.gene77982.t1 MTR_3g053270 22.864 433 288 11 31 440 36 445 1.20e-23 103
MS.gene77982.t1 MTR_3g025340 73.770 61 16 0 88 148 9 69 1.63e-22 90.9
MS.gene77982.t1 MTR_1g086970 24.812 399 225 13 61 442 139 479 6.73e-20 92.4
MS.gene77982.t1 MTR_3g021090 43.411 129 58 4 144 269 297 413 2.93e-19 89.7
MS.gene77982.t1 MTR_6g047750 24.471 425 249 16 31 441 296 662 1.30e-18 89.0
MS.gene77982.t1 MTR_2g099110 22.529 435 255 11 31 441 75 451 3.58e-18 87.0
MS.gene77982.t1 MTR_8g093070 23.038 395 229 12 57 440 165 495 2.94e-17 84.3
MS.gene77982.t1 MTR_0092s0100 31.915 141 94 1 31 171 373 511 4.68e-11 65.5
MS.gene77982.t1 MTR_0092s0100 31.915 141 94 1 31 171 376 514 4.69e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77982.t1 AT1G50420 49.107 448 217 4 7 443 34 481 2.28e-150 436
MS.gene77982.t1 AT1G66350 31.075 428 225 10 21 442 144 507 3.36e-63 213
MS.gene77982.t1 AT5G48150 30.324 432 237 5 16 441 117 490 1.46e-61 207
MS.gene77982.t1 AT5G48150 30.324 432 237 5 16 441 117 490 1.46e-61 207
MS.gene77982.t1 AT5G48150 30.324 432 237 5 16 441 117 490 1.46e-61 207
MS.gene77982.t1 AT5G48150 30.324 432 237 5 16 441 117 490 1.46e-61 207
MS.gene77982.t1 AT3G03450 29.009 424 228 9 31 442 184 546 8.37e-59 202
MS.gene77982.t1 AT1G50600 29.496 417 238 4 31 441 160 526 1.86e-57 197
MS.gene77982.t1 AT2G04890 30.583 412 233 7 31 441 54 413 4.79e-57 194
MS.gene77982.t1 AT1G50600 29.496 417 238 4 31 441 231 597 6.09e-57 198
MS.gene77982.t1 AT1G50600 29.496 417 238 4 31 441 235 601 7.77e-57 197
MS.gene77982.t1 AT2G01570 28.504 421 229 8 31 442 225 582 2.82e-54 191
MS.gene77982.t1 AT1G14920 29.812 426 217 11 31 442 173 530 6.19e-54 188
MS.gene77982.t1 AT4G17230 28.297 417 243 4 31 441 159 525 9.52e-52 182
MS.gene77982.t1 AT4G17230 28.297 417 243 4 31 441 159 525 9.52e-52 182
MS.gene77982.t1 AT3G54220 28.986 414 234 7 33 443 296 652 1.55e-51 184
MS.gene77982.t1 AT1G21450 29.009 424 244 7 25 441 220 593 1.37e-50 181
MS.gene77982.t1 AT1G63100 25.293 427 251 5 31 440 253 628 2.60e-45 167
MS.gene77982.t1 AT1G63100 25.293 427 251 5 31 440 278 653 2.77e-45 167
MS.gene77982.t1 AT2G29060 28.741 421 238 10 31 440 285 654 2.33e-43 161
MS.gene77982.t1 AT2G29060 28.741 421 238 10 31 440 322 691 3.44e-43 161
MS.gene77982.t1 AT1G07530 27.316 421 242 9 31 440 397 764 1.54e-42 160
MS.gene77982.t1 AT5G41920 27.446 419 234 8 31 441 44 400 1.33e-41 152
MS.gene77982.t1 AT5G59450 28.504 421 241 8 31 441 228 598 1.67e-41 155
MS.gene77982.t1 AT3G46600 27.990 418 243 8 31 441 5 371 7.00e-39 144
MS.gene77982.t1 AT3G46600 27.880 434 251 9 19 441 165 547 4.95e-38 145
MS.gene77982.t1 AT3G46600 27.880 434 251 9 19 441 197 579 1.17e-37 145
MS.gene77982.t1 AT1G07520 25.117 426 245 9 34 440 158 528 9.57e-36 139
MS.gene77982.t1 AT1G07520 25.117 426 245 9 34 440 158 528 9.57e-36 139
MS.gene77982.t1 AT1G55580 24.945 453 246 7 31 441 45 445 1.49e-35 137
MS.gene77982.t1 AT1G07520 25.117 426 245 9 34 440 322 692 3.43e-35 139
MS.gene77982.t1 AT1G07520 25.117 426 245 9 34 440 323 693 3.48e-35 138
MS.gene77982.t1 AT2G37650 25.837 418 251 7 31 441 348 713 1.08e-34 137
MS.gene77982.t1 AT2G37650 25.837 418 251 7 31 441 336 701 1.12e-34 137
MS.gene77982.t1 AT5G66770 27.187 423 237 9 31 441 221 584 2.95e-34 135
MS.gene77982.t1 AT2G29065 24.590 427 253 8 31 440 253 627 4.08e-32 129
MS.gene77982.t1 AT3G50650 25.743 404 226 7 53 441 198 542 1.25e-31 127
MS.gene77982.t1 AT4G37650 23.112 437 256 12 31 441 147 529 1.45e-31 127
MS.gene77982.t1 AT5G52510 23.843 432 248 9 31 441 269 640 1.98e-29 121
MS.gene77982.t1 AT5G17490 30.769 208 133 5 31 234 161 361 5.31e-29 119
MS.gene77982.t1 AT5G17490 38.000 150 90 2 295 442 369 517 7.18e-26 110
MS.gene77982.t1 AT3G49950 22.512 422 270 12 31 440 31 407 2.62e-24 104
MS.gene77982.t1 AT4G08250 22.603 438 271 12 17 440 93 476 4.16e-23 102

Find 71 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCAAGTTTGCTAATTAT+TGG 0.118308 3.4:+14350219 None:intergenic
CTGTATCGGCCTGGAAATTT+AGG 0.184893 3.4:-14349510 MS.gene77982:CDS
GACAACCGAAGCAGGGATTT+TGG 0.226524 3.4:-14350262 MS.gene77982:CDS
TCTGAATGGATGTAATTTAA+TGG 0.250687 3.4:-14349872 MS.gene77982:CDS
ATGGATTCAAAGACTTAAAA+TGG 0.281483 3.4:-14349671 MS.gene77982:CDS
CAACTCAGCTGTTATGCATT+AGG 0.310901 3.4:-14350813 MS.gene77982:CDS
TGCTTCTTGGAGAGCAAATA+AGG 0.316928 3.4:-14349750 MS.gene77982:CDS
AAACTCGAGAGCATGCTTCT+TGG 0.323417 3.4:-14349763 MS.gene77982:CDS
TCCAAGTGCTTCACTGAAAT+AGG 0.355279 3.4:+14350611 None:intergenic
TTAAGAAAGATCCCCATCTT+AGG 0.362039 3.4:+14349949 None:intergenic
TAATTGCTTGATTTGTGATC+AGG 0.367971 3.4:+14350460 None:intergenic
CCGCCCCATCTGAAAATTAC+AGG 0.369403 3.4:-14350330 MS.gene77982:CDS
TTTAGGTGCTTCACCTAAGA+TGG 0.374066 3.4:-14349962 MS.gene77982:CDS
ATGATTGTTTACTTGTTTGT+TGG 0.377997 3.4:-14349552 MS.gene77982:CDS
AAGAGGTGATAATGCTGAAT+CGG 0.390322 3.4:+14349987 None:intergenic
GAATCATCACCTAAATTTCC+AGG 0.414889 3.4:+14349501 None:intergenic
AATTCCCTCAGCCAGTGATA+TGG 0.418979 3.4:+14350786 None:intergenic
CACTCAATAAGAGGGATCAT+TGG 0.426274 3.4:+14350738 None:intergenic
CCTTGCCACTGATGACGAAA+TGG 0.436802 3.4:-14350121 MS.gene77982:CDS
TTCAGATGGGGCGGACCTCC+CGG 0.437773 3.4:+14350339 None:intergenic
ATTCAAAGACTTAAAATGGC+TGG 0.439982 3.4:-14349667 MS.gene77982:CDS
CGAATCGATGGAACTTGAAA+AGG 0.443331 3.4:-14350436 MS.gene77982:CDS
ATTATTGGATTGAACTGTAA+AGG 0.444269 3.4:+14350234 None:intergenic
TCAGTAATCACTAAAAGTTT+CGG 0.445565 3.4:+14349907 None:intergenic
TTTCATTTGACAACCGAAGC+AGG 0.447617 3.4:-14350270 MS.gene77982:CDS
GACGAAATGGTCTCCTCAGC+AGG 0.460998 3.4:-14350108 MS.gene77982:CDS
ACAAACTTTAAAGAAACGTC+CGG 0.481008 3.4:-14350358 MS.gene77982:CDS
AAAATCCAAAATCCCTGCTT+CGG 0.483217 3.4:+14350257 None:intergenic
TAAAATGGCTGGATTTATGA+AGG 0.487865 3.4:-14349656 MS.gene77982:CDS
TGTGCAAAATGTGTTGCATC+TGG 0.490537 3.4:-14350717 MS.gene77982:CDS
AGAGGTGATAATGCTGAATC+GGG 0.492776 3.4:+14349988 None:intergenic
TTAGGTGCTTCACCTAAGAT+GGG 0.494167 3.4:-14349961 MS.gene77982:CDS
GAAAACTTGCCTGTCAAGAC+AGG 0.495316 3.4:-14350186 MS.gene77982:CDS
GAACAGGAATCAAATCTGAA+TGG 0.501626 3.4:-14349886 MS.gene77982:CDS
ATTTATCCATTGCGCTGGCT+CGG 0.503400 3.4:+14350386 None:intergenic
ATTTCATCACCTCATGGTAA+TGG 0.506040 3.4:-14350651 MS.gene77982:CDS
TGGTAATGGTGTACAAAGAA+TGG 0.507522 3.4:-14350637 MS.gene77982:CDS
TTGAGAGCTTTGTATACGCC+AGG 0.508327 3.4:+14350567 None:intergenic
CATTAGGTTCGCCATATCAC+TGG 0.511134 3.4:-14350797 MS.gene77982:CDS
ACAGCGTCTCCTGTCTTGAC+AGG 0.513933 3.4:+14350177 None:intergenic
AGGTACCTTTGAGTTACAAT+GGG 0.518881 3.4:-14349636 MS.gene77982:CDS
ACCTATTTCAGTGAAGCACT+TGG 0.523468 3.4:-14350612 MS.gene77982:CDS
TCAAGCAATTATCGAATCGA+TGG 0.531832 3.4:-14350448 MS.gene77982:CDS
ATCAGATTTATCCATTGCGC+TGG 0.534751 3.4:+14350381 None:intergenic
TTGTGAGGGAGTCGATAGAA+AGG 0.541472 3.4:-14349716 MS.gene77982:CDS
AAGGTACCTTTGAGTTACAA+TGG 0.544427 3.4:-14349637 MS.gene77982:CDS
ATTGCGCTGGCTCGGAACAA+TGG 0.551742 3.4:+14350394 None:intergenic
TCTCAGATTTCATCACCTCA+TGG 0.558265 3.4:-14350657 MS.gene77982:CDS
TTCATTTGACAACCGAAGCA+GGG 0.560755 3.4:-14350269 MS.gene77982:CDS
GAGCAGCGCACATGGGTCAG+AGG 0.566671 3.4:-14350062 MS.gene77982:CDS
TACCTTTGAGTTACAATGGG+AGG 0.570596 3.4:-14349633 MS.gene77982:CDS
GGCATGAGAAGCTCGAGCAA+TGG 0.571455 3.4:-14349690 MS.gene77982:CDS
GTTCGCCATATCACTGGCTG+AGG 0.574878 3.4:-14350791 MS.gene77982:CDS
ATCCTCCCATTGTAACTCAA+AGG 0.576632 3.4:+14349631 None:intergenic
TATGCAGAGAGCAGCGCACA+TGG 0.577233 3.4:-14350070 MS.gene77982:CDS
AGGAACATCATTACTTGTGA+GGG 0.584932 3.4:-14349730 MS.gene77982:CDS
ATCGATGGAACTTGAAAAGG+TGG 0.592860 3.4:-14350433 MS.gene77982:CDS
GATAGATGTCGCACCTGCTG+AGG 0.593900 3.4:+14350095 None:intergenic
AAGGAACATCATTACTTGTG+AGG 0.600863 3.4:-14349731 MS.gene77982:CDS
TAGGTGCTTCACCTAAGATG+GGG 0.607732 3.4:-14349960 MS.gene77982:CDS
CCATTTCGTCATCAGTGGCA+AGG 0.614165 3.4:+14350121 None:intergenic
ATTGTTCCGAGCCAGCGCAA+TGG 0.617587 3.4:-14350392 MS.gene77982:CDS
AAGATCGTTAAACATTTACC+TGG 0.629906 3.4:-14350585 MS.gene77982:CDS
CAAACTTTAAAGAAACGTCC+GGG 0.635686 3.4:-14350357 MS.gene77982:CDS
GGAGACCATTTCGTCATCAG+TGG 0.638809 3.4:+14350116 None:intergenic
ATGCAGAGAGCAGCGCACAT+GGG 0.647261 3.4:-14350069 MS.gene77982:CDS
GTCAGAGGACTTTCGCAGAG+TGG 0.652841 3.4:-14350047 MS.gene77982:CDS
TTCGCCATATCACTGGCTGA+GGG 0.658373 3.4:-14350790 MS.gene77982:CDS
AGGGAGTCGATAGAAAGGAA+AGG 0.670114 3.4:-14349711 MS.gene77982:CDS
CTTTGTACACCATTACCATG+AGG 0.671867 3.4:+14350642 None:intergenic
ACTTTAAAGAAACGTCCGGG+AGG 0.713179 3.4:-14350354 MS.gene77982:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGCATAAAAAATGCTGATAT+TGG - chr3.4:14349630-14349649 MS.gene77982:CDS 25.0%
! AGGTATGAAAACTTCAAAAA+AGG + chr3.4:14349846-14349865 None:intergenic 25.0%
! ATGATTGTTTACTTGTTTGT+TGG - chr3.4:14350771-14350790 MS.gene77982:CDS 25.0%
! ATGGATTCAAAGACTTAAAA+TGG - chr3.4:14350652-14350671 MS.gene77982:CDS 25.0%
! ATTATTGGATTGAACTGTAA+AGG + chr3.4:14350092-14350111 None:intergenic 25.0%
! TCAGTAATCACTAAAAGTTT+CGG + chr3.4:14350419-14350438 None:intergenic 25.0%
! TCTGAATGGATGTAATTTAA+TGG - chr3.4:14350451-14350470 MS.gene77982:CDS 25.0%
!! AATTCCTGTAATTTTCAGAT+GGG + chr3.4:14350000-14350019 None:intergenic 25.0%
!!! CATAGAAGTATTTTTGAACA+AGG + chr3.4:14349815-14349834 None:intergenic 25.0%
!!! TCTTCAAGTTTGCTAATTAT+TGG + chr3.4:14350107-14350126 None:intergenic 25.0%
AAGATCGTTAAACATTTACC+TGG - chr3.4:14349738-14349757 MS.gene77982:CDS 30.0%
ATTCAAAGACTTAAAATGGC+TGG - chr3.4:14350656-14350675 MS.gene77982:CDS 30.0%
TAAAATGGCTGGATTTATGA+AGG - chr3.4:14350667-14350686 MS.gene77982:CDS 30.0%
! ACAAACTTTAAAGAAACGTC+CGG - chr3.4:14349965-14349984 MS.gene77982:CDS 30.0%
! ACTTTTAGTGATTACTGAAC+AGG - chr3.4:14350421-14350440 MS.gene77982:CDS 30.0%
! ATTCCTGTAATTTTCAGATG+GGG + chr3.4:14349999-14350018 None:intergenic 30.0%
! ATTTTGCACACTCAATAAGA+GGG + chr3.4:14349596-14349615 None:intergenic 30.0%
! GAATTCCTGTAATTTTCAGA+TGG + chr3.4:14350001-14350020 None:intergenic 30.0%
!! TAATTGCTTGATTTGTGATC+AGG + chr3.4:14349866-14349885 None:intergenic 30.0%
!!! GCATTATCACCTCTTTTTTT+AGG - chr3.4:14350344-14350363 MS.gene77982:CDS 30.0%
AAAATCCAAAATCCCTGCTT+CGG + chr3.4:14350069-14350088 None:intergenic 35.0%
AAGGAACATCATTACTTGTG+AGG - chr3.4:14350592-14350611 MS.gene77982:CDS 35.0%
AGGAACATCATTACTTGTGA+GGG - chr3.4:14350593-14350612 MS.gene77982:CDS 35.0%
ATTTCATCACCTCATGGTAA+TGG - chr3.4:14349672-14349691 MS.gene77982:CDS 35.0%
GAACAGGAATCAAATCTGAA+TGG - chr3.4:14350437-14350456 MS.gene77982:CDS 35.0%
TCAAGCAATTATCGAATCGA+TGG - chr3.4:14349875-14349894 MS.gene77982:CDS 35.0%
TTAAGAAAGATCCCCATCTT+AGG + chr3.4:14350377-14350396 None:intergenic 35.0%
! AAGAGGTGATAATGCTGAAT+CGG + chr3.4:14350339-14350358 None:intergenic 35.0%
! AAGGTACCTTTGAGTTACAA+TGG - chr3.4:14350686-14350705 MS.gene77982:CDS 35.0%
! AGGTACCTTTGAGTTACAAT+GGG - chr3.4:14350687-14350706 MS.gene77982:CDS 35.0%
! CAAACTTTAAAGAAACGTCC+GGG - chr3.4:14349966-14349985 MS.gene77982:CDS 35.0%
! CATTTTGCACACTCAATAAG+AGG + chr3.4:14349597-14349616 None:intergenic 35.0%
! TGAAGACGATGATATTTTCG+AGG + chr3.4:14349784-14349803 None:intergenic 35.0%
!! GATCGAGCATTGTACTTTTA+CGG - chr3.4:14350482-14350501 MS.gene77982:CDS 35.0%
!! TGGTAATGGTGTACAAAGAA+TGG - chr3.4:14349686-14349705 MS.gene77982:CDS 35.0%
!!! CAGACCACTTTTTTCTGTAT+CGG - chr3.4:14350799-14350818 MS.gene77982:CDS 35.0%
ATCAGATTTATCCATTGCGC+TGG + chr3.4:14349945-14349964 None:intergenic 40.0%
ATCCTCCCATTGTAACTCAA+AGG + chr3.4:14350695-14350714 None:intergenic 40.0%
ATCGATGGAACTTGAAAAGG+TGG - chr3.4:14349890-14349909 MS.gene77982:CDS 40.0%
CAACTCAGCTGTTATGCATT+AGG - chr3.4:14349510-14349529 MS.gene77982:CDS 40.0%
CACTCAATAAGAGGGATCAT+TGG + chr3.4:14349588-14349607 None:intergenic 40.0%
CGAATCGATGGAACTTGAAA+AGG - chr3.4:14349887-14349906 MS.gene77982:CDS 40.0%
CTTTGTACACCATTACCATG+AGG + chr3.4:14349684-14349703 None:intergenic 40.0%
TCCAAGTGCTTCACTGAAAT+AGG + chr3.4:14349715-14349734 None:intergenic 40.0%
TCTCAGATTTCATCACCTCA+TGG - chr3.4:14349666-14349685 MS.gene77982:CDS 40.0%
TGCTTCTTGGAGAGCAAATA+AGG - chr3.4:14350573-14350592 MS.gene77982:CDS 40.0%
TGTGCAAAATGTGTTGCATC+TGG - chr3.4:14349606-14349625 MS.gene77982:CDS 40.0%
TTAGGTGCTTCACCTAAGAT+GGG - chr3.4:14350362-14350381 MS.gene77982:CDS 40.0%
TTCATTTGACAACCGAAGCA+GGG - chr3.4:14350054-14350073 MS.gene77982:CDS 40.0%
TTTAGGTGCTTCACCTAAGA+TGG - chr3.4:14350361-14350380 MS.gene77982:CDS 40.0%
TTTCATTTGACAACCGAAGC+AGG - chr3.4:14350053-14350072 MS.gene77982:CDS 40.0%
! ACCTATTTCAGTGAAGCACT+TGG - chr3.4:14349711-14349730 MS.gene77982:CDS 40.0%
! AGAGGTGATAATGCTGAATC+GGG + chr3.4:14350338-14350357 None:intergenic 40.0%
! GGTGAAGCACCTAAAAAAAG+AGG + chr3.4:14350356-14350375 None:intergenic 40.0%
! TACCTTTGAGTTACAATGGG+AGG - chr3.4:14350690-14350709 MS.gene77982:CDS 40.0%
AATTCCCTCAGCCAGTGATA+TGG + chr3.4:14349540-14349559 None:intergenic 45.0%
AGGGAGTCGATAGAAAGGAA+AGG - chr3.4:14350612-14350631 MS.gene77982:CDS 45.0%
CAGGCCGATACAGAAAAAAG+TGG + chr3.4:14350806-14350825 None:intergenic 45.0%
CATTAGGTTCGCCATATCAC+TGG - chr3.4:14349526-14349545 MS.gene77982:CDS 45.0%
GAAAACTTGCCTGTCAAGAC+AGG - chr3.4:14350137-14350156 MS.gene77982:CDS 45.0%
TAGGTGCTTCACCTAAGATG+GGG - chr3.4:14350363-14350382 MS.gene77982:CDS 45.0%
TTGTGAGGGAGTCGATAGAA+AGG - chr3.4:14350607-14350626 MS.gene77982:CDS 45.0%
! AAACTCGAGAGCATGCTTCT+TGG - chr3.4:14350560-14350579 MS.gene77982:CDS 45.0%
! ACTTTAAAGAAACGTCCGGG+AGG - chr3.4:14349969-14349988 MS.gene77982:CDS 45.0%
! CCTGTAATTTTCAGATGGGG+CGG + chr3.4:14349996-14350015 None:intergenic 45.0%
! CTGTATCGGCCTGGAAATTT+AGG - chr3.4:14350813-14350832 MS.gene77982:CDS 45.0%
! TTGAGAGCTTTGTATACGCC+AGG + chr3.4:14349759-14349778 None:intergenic 45.0%
!! ATTTATCCATTGCGCTGGCT+CGG + chr3.4:14349940-14349959 None:intergenic 45.0%
!!! CACTTTTTTCTGTATCGGCC+TGG - chr3.4:14350804-14350823 MS.gene77982:CDS 45.0%
!!! CGGTTCACTTTTTGACTGCT+TGG - chr3.4:14350502-14350521 MS.gene77982:CDS 45.0%
CCATTTCGTCATCAGTGGCA+AGG + chr3.4:14350205-14350224 None:intergenic 50.0%
CCGCCCCATCTGAAAATTAC+AGG - chr3.4:14349993-14350012 MS.gene77982:CDS 50.0%
GGAGACCATTTCGTCATCAG+TGG + chr3.4:14350210-14350229 None:intergenic 50.0%
! CCTTGCCACTGATGACGAAA+TGG - chr3.4:14350202-14350221 MS.gene77982:CDS 50.0%
! GACAACCGAAGCAGGGATTT+TGG - chr3.4:14350061-14350080 MS.gene77982:CDS 50.0%
! TTCGCCATATCACTGGCTGA+GGG - chr3.4:14349533-14349552 MS.gene77982:CDS 50.0%
ACAGCGTCTCCTGTCTTGAC+AGG + chr3.4:14350149-14350168 None:intergenic 55.0%
ATGCAGAGAGCAGCGCACAT+GGG - chr3.4:14350254-14350273 MS.gene77982:CDS 55.0%
ATTGTTCCGAGCCAGCGCAA+TGG - chr3.4:14349931-14349950 MS.gene77982:CDS 55.0%
GACGAAATGGTCTCCTCAGC+AGG - chr3.4:14350215-14350234 MS.gene77982:CDS 55.0%
GATAGATGTCGCACCTGCTG+AGG + chr3.4:14350231-14350250 None:intergenic 55.0%
GGCATGAGAAGCTCGAGCAA+TGG - chr3.4:14350633-14350652 MS.gene77982:CDS 55.0%
GTCAGAGGACTTTCGCAGAG+TGG - chr3.4:14350276-14350295 MS.gene77982:CDS 55.0%
TATGCAGAGAGCAGCGCACA+TGG - chr3.4:14350253-14350272 MS.gene77982:CDS 55.0%
! GTTCGCCATATCACTGGCTG+AGG - chr3.4:14349532-14349551 MS.gene77982:CDS 55.0%
!! ATTGCGCTGGCTCGGAACAA+TGG + chr3.4:14349932-14349951 None:intergenic 55.0%
GAGCAGCGCACATGGGTCAG+AGG - chr3.4:14350261-14350280 MS.gene77982:CDS 65.0%
TTCAGATGGGGCGGACCTCC+CGG + chr3.4:14349987-14350006 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 14349507 14350838 14349507 ID=MS.gene77982
chr3.4 mRNA 14349507 14350838 14349507 ID=MS.gene77982.t1;Parent=MS.gene77982
chr3.4 exon 14349507 14350838 14349507 ID=MS.gene77982.t1.exon1;Parent=MS.gene77982.t1
chr3.4 CDS 14349507 14350838 14349507 ID=cds.MS.gene77982.t1;Parent=MS.gene77982.t1
Gene Sequence

>MS.gene77982

ATGCAACTCAGCTGTTATGCATTAGGTTCGCCATATCACTGGCTGAGGGAATTGAGATATGACTCTCAGAGCTCAAATCCAATGATCCCTCTTATTGAGTGTGCAAAATGTGTTGCATCTGGAAGCATAAAAAATGCTGATATTGGACTTGAGTATATTTCTCAGATTTCATCACCTCATGGTAATGGTGTACAAAGAATGGTCACCTATTTCAGTGAAGCACTTGGATACAAGATCGTTAAACATTTACCTGGCGTATACAAAGCTCTCAATTCCTCGAAAATATCATCGTCTTCAGATGATATCCTTGTTCAAAAATACTTCTATGATCTGTGTCCTTTTTTGAAGTTTTCATACCTGATCACAAATCAAGCAATTATCGAATCGATGGAACTTGAAAAGGTGGTTCATATTATCGATCTCCATTGTTCCGAGCCAGCGCAATGGATAAATCTGATACAAACTTTAAAGAAACGTCCGGGAGGTCCGCCCCATCTGAAAATTACAGGAATTCATGAAAAGAAAGAAGTATTAGAGCAAATGAGCTTTCATTTGACAACCGAAGCAGGGATTTTGGATTTTCCTTTACAGTTCAATCCAATAATTAGCAAACTTGAAGATGTCGATTTCGAAAACTTGCCTGTCAAGACAGGAGACGCTGTCGCAATTTCTTCTGTTCTTCAGCTGCATTCTCTCCTTGCCACTGATGACGAAATGGTCTCCTCAGCAGGTGCGACATCTATCAATATGCAGAGAGCAGCGCACATGGGTCAGAGGACTTTCGCAGAGTGGCTAGAGAGAGACATGATCAATGCATATATCTTGAGTCCCGATTCAGCATTATCACCTCTTTTTTTAGGTGCTTCACCTAAGATGGGGATCTTTCTTAACGCTATGCGAAAACTACAACCGAAACTTTTAGTGATTACTGAACAGGAATCAAATCTGAATGGATGTAATTTAATGGAGAGAATCGATCGAGCATTGTACTTTTACGGTTCACTTTTTGACTGCTTGGAATCTACTGTTACGAGAACATCAGTCGAGAGACAAAAACTCGAGAGCATGCTTCTTGGAGAGCAAATAAGGAACATCATTACTTGTGAGGGAGTCGATAGAAAGGAAAGGCATGAGAAGCTCGAGCAATGGATTCAAAGACTTAAAATGGCTGGATTTATGAAGGTACCTTTGAGTTACAATGGGAGGATAGAAGCGACAAATCTATTGCAGCGATATAGTCATAAATACAAGTTTAAAGAAGAAAATGATTGTTTACTTGTTTGTTGGAGTGACAGACCACTTTTTTCTGTATCGGCCTGGAAATTTAGGTGA
Protein sequence

>MS.gene77982.t1

MQLSCYALGSPYHWLRELRYDSQSSNPMIPLIECAKCVASGSIKNADIGLEYISQISSPHGNGVQRMVTYFSEALGYKIVKHLPGVYKALNSSKISSSSDDILVQKYFYDLCPFLKFSYLITNQAIIESMELEKVVHIIDLHCSEPAQWINLIQTLKKRPGGPPHLKITGIHEKKEVLEQMSFHLTTEAGILDFPLQFNPIISKLEDVDFENLPVKTGDAVAISSVLQLHSLLATDDEMVSSAGATSINMQRAAHMGQRTFAEWLERDMINAYILSPDSALSPLFLGASPKMGIFLNAMRKLQPKLLVITEQESNLNGCNLMERIDRALYFYGSLFDCLESTVTRTSVERQKLESMLLGEQIRNIITCEGVDRKERHEKLEQWIQRLKMAGFMKVPLSYNGRIEATNLLQRYSHKYKFKEENDCLLVCWSDRPLFSVSAWKFR