AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78516

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78516.t1 MTR_5g065200 75.644 854 155 9 22 860 19 834 0.0 1264
MS.gene78516.t1 MTR_1g114150 74.428 743 162 6 123 860 331 1050 0.0 1151
MS.gene78516.t1 MTR_1g114130 72.861 748 185 6 116 862 310 1040 0.0 1123
MS.gene78516.t1 MTR_1g069565 64.821 867 250 11 40 860 24 881 0.0 1078
MS.gene78516.t1 MTR_1g114070 68.207 736 206 4 126 860 216 924 0.0 1034
MS.gene78516.t1 MTR_1g069565 60.281 924 255 14 40 860 24 938 0.0 1030
MS.gene78516.t1 MTR_1g114150 71.835 632 150 6 123 749 331 939 0.0 940
MS.gene78516.t1 MTR_5g047620 66.617 668 194 11 207 860 145 797 0.0 878
MS.gene78516.t1 MTR_0020s0100 61.751 651 197 11 203 843 83 691 0.0 773
MS.gene78516.t1 MTR_7g117445 45.839 805 345 15 131 859 136 925 0.0 667
MS.gene78516.t1 MTR_7g117445 45.839 805 345 15 131 859 136 925 0.0 666
MS.gene78516.t1 MTR_7g117445 45.839 805 345 15 131 859 136 925 0.0 666
MS.gene78516.t1 MTR_4g132540 44.602 778 348 15 110 861 147 867 0.0 630
MS.gene78516.t1 MTR_2g024270 46.730 734 323 12 129 858 148 817 0.0 606
MS.gene78516.t1 MTR_7g117445 44.430 745 323 15 131 799 136 865 0.0 589
MS.gene78516.t1 MTR_2g024140 45.652 736 318 13 128 858 27 685 0.0 587
MS.gene78516.t1 MTR_1g083120 36.913 745 406 14 135 862 198 895 6.78e-153 473
MS.gene78516.t1 MTR_1g083120 36.913 745 406 14 135 862 215 912 9.40e-153 473
MS.gene78516.t1 MTR_1g083120 36.550 684 370 14 135 801 198 834 1.55e-134 422
MS.gene78516.t1 MTR_1g083120 36.550 684 370 14 135 801 198 834 2.09e-134 422
MS.gene78516.t1 MTR_2g011630 34.805 770 432 19 135 857 207 953 4.78e-132 419
MS.gene78516.t1 MTR_1g008060 43.123 538 266 11 129 661 855 1357 5.23e-127 419
MS.gene78516.t1 MTR_1g008060 72.059 136 38 0 714 849 1542 1677 1.25e-57 216
MS.gene78516.t1 MTR_1g008060 43.123 538 266 11 129 661 864 1366 5.60e-127 419
MS.gene78516.t1 MTR_1g008060 72.059 136 38 0 714 849 1551 1686 1.26e-57 216
MS.gene78516.t1 MTR_2g011630 35.191 682 382 16 219 857 45 709 8.24e-118 375
MS.gene78516.t1 MTR_2g011630 35.191 682 382 16 219 857 77 741 1.31e-117 375
MS.gene78516.t1 MTR_8g062940 51.657 362 142 4 135 496 33 361 2.36e-114 353
MS.gene78516.t1 MTR_2g024240 54.019 311 107 3 513 823 114 388 1.79e-105 331
MS.gene78516.t1 MTR_4g091520 36.121 562 286 15 359 857 727 1278 6.88e-94 322
MS.gene78516.t1 MTR_4g091520 36.310 168 100 3 135 297 260 425 1.04e-25 115
MS.gene78516.t1 MTR_0295s0020 58.152 184 70 1 486 669 1 177 7.73e-62 207
MS.gene78516.t1 MTR_4g091520 32.632 475 250 14 359 773 657 1121 5.23e-59 219
MS.gene78516.t1 MTR_4g091520 36.310 168 100 3 135 297 260 425 7.41e-26 115
MS.gene78516.t1 MTR_4g091520 32.632 475 250 14 359 773 727 1191 6.98e-59 219
MS.gene78516.t1 MTR_4g091520 36.310 168 100 3 135 297 260 425 6.67e-26 115
MS.gene78516.t1 MTR_4g091520 32.632 475 250 14 359 773 692 1156 7.18e-59 219
MS.gene78516.t1 MTR_4g091520 36.310 168 100 3 135 297 260 425 7.32e-26 115
MS.gene78516.t1 MTR_8g465700 51.795 195 87 2 448 642 4 191 7.93e-58 199
MS.gene78516.t1 MTR_8g013920 60.256 156 43 3 538 693 40 176 1.63e-42 154
MS.gene78516.t1 MTR_8g465720 75.000 88 21 1 714 801 53 139 1.44e-40 147
MS.gene78516.t1 MTR_3g080750 57.377 61 20 1 624 678 85 145 5.35e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78516.t1 AT1G11950 48.098 736 326 10 131 862 189 872 0.0 674
MS.gene78516.t1 AT1G11950 48.098 736 326 10 131 862 189 872 0.0 674
MS.gene78516.t1 AT3G07610 45.207 772 340 8 134 857 154 890 0.0 662
MS.gene78516.t1 AT1G62310 46.433 743 343 13 110 842 181 878 0.0 655
MS.gene78516.t1 AT3G07610 43.995 791 344 9 134 857 154 912 0.0 641
MS.gene78516.t1 AT4G00990 42.692 780 361 17 135 858 80 829 0.0 590
MS.gene78516.t1 AT1G11950 46.015 665 303 10 131 791 189 801 0.0 569
MS.gene78516.t1 AT4G00990 41.427 729 341 17 135 807 80 778 1.12e-174 524
MS.gene78516.t1 AT4G00990 41.748 721 334 17 135 799 80 770 9.02e-174 522
MS.gene78516.t1 AT3G07610 42.125 546 259 6 134 631 154 690 1.65e-136 425
MS.gene78516.t1 AT1G09060 31.921 755 437 17 128 862 197 894 9.84e-117 377
MS.gene78516.t1 AT1G09060 31.921 755 437 17 128 862 197 894 9.84e-117 377
MS.gene78516.t1 AT1G09060 31.921 755 437 17 128 862 211 908 1.40e-116 377
MS.gene78516.t1 AT1G09060 31.921 755 437 17 128 862 211 908 1.40e-116 377
MS.gene78516.t1 AT4G21430 29.933 745 452 14 129 857 221 911 7.22e-95 318

Find 190 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACATTTATTCTTAGATTT+TGG 0.128373 5.1:+60876220 None:intergenic
AGTGCTCTCTGAAGCATTTA+TGG 0.163893 5.1:+60874545 None:intergenic
GGATTTGTATATTCTTTAAA+TGG 0.213495 5.1:+60875343 None:intergenic
TCATATTGCTAAAGCAAAAT+TGG 0.216686 5.1:-60874676 MS.gene78516:CDS
GGTTGGTGGTTCTACGTTCT+TGG 0.246061 5.1:+60877653 None:intergenic
GATCTTCATCACGAGGATTT+AGG 0.246459 5.1:-60875962 MS.gene78516:CDS
CTTCGTTGTGGCCAACTTCT+AGG 0.252701 5.1:-60876415 MS.gene78516:CDS
CTATCCTTCATCGCTATTCT+AGG 0.275882 5.1:-60877713 MS.gene78516:CDS
AGTACATTGCTGACAATTAC+AGG 0.278275 5.1:+60875908 None:intergenic
TGTCATTTCTTTGACATTGA+TGG 0.280482 5.1:+60877293 None:intergenic
TGTCTTGCTTAAGGTGCCTT+TGG 0.287167 5.1:+60874619 None:intergenic
ACTAAGTCAGAGATGTAATT+TGG 0.287365 5.1:+60876163 None:intergenic
GACCAAGCACAAGCCACTTT+TGG 0.295143 5.1:-60875819 MS.gene78516:CDS
GATGATGATGGCCAATCTTC+TGG 0.296228 5.1:-60874522 MS.gene78516:CDS
CAGCTCTTTGCGTAGATTCC+AGG 0.304385 5.1:-60876015 MS.gene78516:CDS
TACCTCCAATTTGTCCTCTA+TGG 0.305460 5.1:+60871567 None:intergenic
TCTGGCTTTAGAACATTATC+AGG 0.310569 5.1:+60875286 None:intergenic
AGTACGCGCGCTCATCATTA+TGG 0.311143 5.1:+60876617 None:intergenic
TCTACCACATTTAATACAAT+TGG 0.320615 5.1:-60876865 MS.gene78516:CDS
GCTCTTGTTTGAAGCCTGTT+AGG 0.324611 5.1:-60876072 MS.gene78516:CDS
GTCTTGCTTAAGGTGCCTTT+GGG 0.327340 5.1:+60874620 None:intergenic
TCTATGGACCTGTGATTTAC+TGG 0.329135 5.1:+60871583 None:intergenic
GCTCCCTCTTGTCTTGCTTA+AGG 0.329503 5.1:+60874610 None:intergenic
ATTCTTTAGATGGTGCTCTC+TGG 0.331445 5.1:-60874437 MS.gene78516:CDS
AGCATGAGGTGCTTTGTATC+TGG 0.334250 5.1:+60876315 None:intergenic
AATGTGCACCAATTCTTTAC+CGG 0.348314 5.1:-60875490 MS.gene78516:CDS
CAAGAAAATGGTTTGTTGGT+TGG 0.352054 5.1:-60874462 MS.gene78516:CDS
CGTCCATTGCTCTCCTTTAG+AGG 0.359183 5.1:-60874343 MS.gene78516:CDS
CAACACAATTCGTCGTAATA+AGG 0.364645 5.1:-60877445 MS.gene78516:intron
CTTTCCTCAAATAAATTAGA+AGG 0.368022 5.1:+60875403 None:intergenic
ATGCCGGACGGTTAGATAAA+AGG 0.368269 5.1:+60873070 None:intergenic
GCGACGATGAAACAAGAAAA+TGG 0.376184 5.1:-60874474 MS.gene78516:CDS
TTGGTTGGAGATTCTTTAGA+TGG 0.389380 5.1:-60874447 MS.gene78516:CDS
ATACGTACATGGTCAAAATC+TGG 0.391419 5.1:-60876277 MS.gene78516:CDS
AGAGGACGTACCCGCATTGC+AGG 0.392255 5.1:-60874400 MS.gene78516:CDS
ATTTATGGAAGAAGACAAAT+TGG 0.392384 5.1:+60874560 None:intergenic
AAGATAAACTCAAAATCAAA+TGG 0.394363 5.1:+60876382 None:intergenic
GCCTCCTTCTAATTTATTTG+AGG 0.400880 5.1:-60875407 MS.gene78516:CDS
ATGTCATCTCTGTTCCTAAC+AGG 0.405147 5.1:+60876058 None:intergenic
AGGCCAATCCTGTTTATCCT+TGG 0.405342 5.1:+60875453 None:intergenic
GGCCAATCCTGTTTATCCTT+GGG 0.421151 5.1:+60875454 None:intergenic
GGAGCTACATGGTGATAATC+AGG 0.421783 5.1:-60874592 MS.gene78516:CDS
AATGTACTTGAATGTACATC+TGG 0.421952 5.1:-60875893 MS.gene78516:CDS
ATATAAAATACCTTGTATCT+TGG 0.422375 5.1:+60876809 None:intergenic
TTCCACCATGCAGATAATCT+TGG 0.430174 5.1:+60876353 None:intergenic
GAGTATTGAAATTGAAAGAT+TGG 0.430433 5.1:-60875429 MS.gene78516:CDS
CCTTCAAATTTCTGACTTGG+TGG 0.430574 5.1:+60872857 None:intergenic
AGAAGCATTTCAAAGAGTTC+AGG 0.434223 5.1:-60874368 MS.gene78516:CDS
TTGTGTACCCGGTAAAGAAT+TGG 0.436382 5.1:+60875482 None:intergenic
TTCAGATATTCCTGCAATGC+GGG 0.438768 5.1:+60874390 None:intergenic
GAAAATGATTATATACACTA+TGG 0.439229 5.1:-60870955 MS.gene78516:CDS
AAATTGTCCCAAGGATAAAC+AGG 0.439965 5.1:-60875461 MS.gene78516:CDS
TTCTTTAGATGGTGCTCTCT+GGG 0.441915 5.1:-60874436 MS.gene78516:CDS
AAGAGCTTTGAGCATATACT+TGG 0.442351 5.1:+60876887 None:intergenic
CGTTAAACGCAACCGTAAAA+CGG 0.451202 5.1:-60877628 MS.gene78516:CDS
GAAATATGCTTCTCAGTTCA+AGG 0.451338 5.1:+60876188 None:intergenic
AATGTACATCTGGTTTAAGC+TGG 0.451500 5.1:-60875883 MS.gene78516:CDS
TTCAAATTTCTGACTTGGTG+GGG 0.453619 5.1:+60872859 None:intergenic
CTTCAAATTTCTGACTTGGT+GGG 0.454550 5.1:+60872858 None:intergenic
AACAAAATCAAGTGCAACCT+TGG 0.456526 5.1:+60871660 None:intergenic
CCACCAAGTCAGAAATTTGA+AGG 0.456844 5.1:-60872857 MS.gene78516:intron
CAGGTCCATAGAGGACAAAT+TGG 0.466017 5.1:-60871572 MS.gene78516:CDS
GAAACAAGAAAATGGTTTGT+TGG 0.467660 5.1:-60874466 MS.gene78516:CDS
AATTCAGAAATGGCGTTGGC+GGG 0.468398 5.1:-60877810 None:intergenic
AGGCGCTTGAATGCTTAGAT+TGG 0.470973 5.1:-60875790 MS.gene78516:CDS
ATAATGATGAGCGCGCGTAC+TGG 0.472042 5.1:-60876615 MS.gene78516:intron
CTCAACCTGATGATCATTGT+TGG 0.473165 5.1:+60876914 None:intergenic
TCTAAGAATAAATGTGATCA+TGG 0.477946 5.1:-60876214 MS.gene78516:CDS
TCTTCCTAGAATAGCGATGA+AGG 0.478322 5.1:+60877709 None:intergenic
TGACCTCTGAGCCAGAAGAT+TGG 0.482683 5.1:+60874511 None:intergenic
CGATACCAACAATGATCATC+AGG 0.484087 5.1:-60876919 MS.gene78516:CDS
AAAAGAACTTGCAGATTCAA+TGG 0.485093 5.1:-60876131 MS.gene78516:CDS
TGCGGAATTCCTCTGTCAAA+CGG 0.487229 5.1:+60871611 None:intergenic
TGTGATTTACTGGAAGTTTG+CGG 0.491065 5.1:+60871593 None:intergenic
GAACGGTTCATGCGACGCCT+TGG 0.493522 5.1:-60877754 MS.gene78516:CDS
ATCTTCTGGCTCAGAGGTCA+AGG 0.494381 5.1:-60874508 MS.gene78516:CDS
TTCAATGGCACTGGAGCAGA+GGG 0.494515 5.1:-60875934 MS.gene78516:CDS
GTTCTTTAGGTATCCTCACT+TGG 0.494596 5.1:-60877137 MS.gene78516:intron
TTACCTTCAAATTTCTGACT+TGG 0.497862 5.1:+60872854 None:intergenic
GAATAGCGATGAAGGATAGA+TGG 0.502947 5.1:+60877717 None:intergenic
GAATTCAGAAATGGCGTTGG+CGG 0.503051 5.1:-60877811 None:intergenic
GTGTATGCTGTATTAACAAA+TGG 0.503342 5.1:-60877225 MS.gene78516:intron
TCAAATTTCTGACTTGGTGG+GGG 0.505542 5.1:+60872860 None:intergenic
GTCAAAGAAATGACAAAGGA+AGG 0.505650 5.1:-60877285 MS.gene78516:CDS
CGGTTGCGTTTAACGGTGGT+TGG 0.507184 5.1:+60877636 None:intergenic
ATCCAAAAGTGGCTTGTGCT+TGG 0.509797 5.1:+60875817 None:intergenic
TGTATATTCTTTAAATGGCA+AGG 0.512473 5.1:+60875348 None:intergenic
AGCTCTTTGCGTAGATTCCA+GGG 0.513640 5.1:-60876014 MS.gene78516:CDS
GCAAAGGGTGTTTGAGATCA+CGG 0.514544 5.1:-60877063 MS.gene78516:CDS
CATGCAGATAATCTTGGCCT+CGG 0.514765 5.1:+60876359 None:intergenic
AGATAAACTCAAAATCAAAT+GGG 0.515679 5.1:+60876383 None:intergenic
CCATGTACGTATCTCAAGTT+CGG 0.518603 5.1:+60876288 None:intergenic
TGTATGTAGGAATTGAGCCT+TGG 0.523387 5.1:-60872930 MS.gene78516:intron
CGCAACCGTAAAACGGAAGA+AGG 0.528137 5.1:-60877621 MS.gene78516:CDS
CTTGGGACAATTTGTGTACC+CGG 0.535581 5.1:+60875471 None:intergenic
ACTAACTTTACCTCCTCTAA+AGG 0.536444 5.1:+60874330 None:intergenic
TTAGATAAAAGGTCTGATCA+TGG 0.536799 5.1:+60873081 None:intergenic
TTGCGTTTAACGGTGGTTGG+TGG 0.543542 5.1:+60877639 None:intergenic
ACAAACACATCTTATCATGC+TGG 0.544179 5.1:-60877540 MS.gene78516:CDS
GAGATAGAAGCCAAGATACA+AGG 0.546758 5.1:-60876819 MS.gene78516:intron
AGCGCCTTAACATCCAAAAG+TGG 0.548428 5.1:+60875806 None:intergenic
ATGTGCACCAATTCTTTACC+GGG 0.551064 5.1:-60875489 MS.gene78516:CDS
TGATGAAGATCTACTGCCCT+AGG 0.552902 5.1:+60875974 None:intergenic
TAAAAGGTCTGATCATGGAT+GGG 0.555220 5.1:+60873086 None:intergenic
AGACATATCACAATGGAGCT+TGG 0.555405 5.1:+60875198 None:intergenic
TTGCAATTACCACAACATAC+AGG 0.555718 5.1:+60877088 None:intergenic
GGTCTGCACCACCTAGAAGT+TGG 0.555830 5.1:+60876404 None:intergenic
ATAAAAGGTCTGATCATGGA+TGG 0.557609 5.1:+60873085 None:intergenic
TCTTTCAATTTCAATACTCG+AGG 0.562439 5.1:+60875433 None:intergenic
AGGTTGAGAGCACCTTTGAA+AGG 0.563537 5.1:+60875322 None:intergenic
TACGTACATGGTCAAAATCT+GGG 0.563636 5.1:-60876276 MS.gene78516:CDS
GTGGTAATTGCAATTGCAAA+GGG 0.564159 5.1:-60877078 MS.gene78516:CDS
CAACAATGATCATCAGGTTG+AGG 0.564275 5.1:-60876913 MS.gene78516:CDS
ACGCAGAACAAACGCGACGA+AGG 0.566903 5.1:-60877685 MS.gene78516:CDS
GTCCCAAGGATAAACAGGAT+TGG 0.567361 5.1:-60875456 MS.gene78516:CDS
GAATATACAAATCCTTTCAA+AGG 0.567529 5.1:-60875334 MS.gene78516:CDS
AACGAAGCTCGCGGCAACAG+AGG 0.568999 5.1:+60876431 None:intergenic
AGATTATCTGCATGGTGGAA+AGG 0.569015 5.1:-60876350 MS.gene78516:CDS
TTAATTGCATCAATACTCGC+AGG 0.570155 5.1:+60874651 None:intergenic
ATGTACATCTGGTTTAAGCT+GGG 0.572127 5.1:-60875882 MS.gene78516:CDS
ATTTGATCTTCTTCCAAGTG+AGG 0.572865 5.1:+60877124 None:intergenic
ATGACATACACAATAACTCA+AGG 0.573104 5.1:-60876042 MS.gene78516:CDS
TTTCAATGGCACTGGAGCAG+AGG 0.573700 5.1:-60875935 MS.gene78516:CDS
TGGTCTCTCAGTCGCAGCCA+AGG 0.578123 5.1:+60877737 None:intergenic
TTTGAATTCAGAAATGGCGT+TGG 0.581594 5.1:-60877814 None:intergenic
GCTGGGAGCCGCTTGTCATG+TGG 0.582104 5.1:-60875865 MS.gene78516:CDS
TCAACAACAAATGTCTGAGA+AGG 0.585137 5.1:-60876841 MS.gene78516:CDS
GTCCATAGAGGACAAATTGG+AGG 0.587047 5.1:-60871569 MS.gene78516:intron
CAAAACATTAGAGAAAGCAT+TGG 0.588335 5.1:-60870922 MS.gene78516:CDS
AGTAAATCACAGGTCCATAG+AGG 0.591019 5.1:-60871581 MS.gene78516:CDS
AGCTTCTGAACAAAAGTCCA+AGG 0.591626 5.1:+60872913 None:intergenic
CGAGGCCAAGATTATCTGCA+TGG 0.592075 5.1:-60876358 MS.gene78516:CDS
TATCAGGCAGCTTCACAGCA+AGG 0.593858 5.1:+60875302 None:intergenic
CAAGACAAGAGGGAGCTACA+TGG 0.594046 5.1:-60874603 MS.gene78516:CDS
ACGACGGCGTCATCGTTGAA+CGG 0.597980 5.1:-60877771 MS.gene78516:CDS
GCAAACTTCCAGTAAATCAC+AGG 0.600882 5.1:-60871591 MS.gene78516:CDS
AAAAGGTCTGATCATGGATG+GGG 0.603357 5.1:+60873087 None:intergenic
GCACCTTAAGCAAGACAAGA+GGG 0.606408 5.1:-60874613 MS.gene78516:CDS
TCCTCAAATAAATTAGAAGG+AGG 0.607054 5.1:+60875406 None:intergenic
TCTGCATGGTGGAAAGGCGG+AGG 0.608026 5.1:-60876344 MS.gene78516:CDS
GAAGATCTACTGCCCTAGGA+TGG 0.610352 5.1:+60875978 None:intergenic
TCGAACAACAACAGCAGCAA+CGG 0.610845 5.1:+60877584 None:intergenic
TTGTTGTTCGAAAGAGTGAA+CGG 0.611861 5.1:-60877572 MS.gene78516:CDS
TAATGTTCTAAAGCCAGACA+TGG 0.612070 5.1:-60875281 MS.gene78516:CDS
TCAGAGAGCACTGATGATGA+TGG 0.614655 5.1:-60874534 MS.gene78516:CDS
TGTTGCCGCGAGCTTCGTTG+TGG 0.615230 5.1:-60876427 MS.gene78516:CDS
AGATACAAAGCACCTCATGC+TGG 0.616281 5.1:-60876313 MS.gene78516:CDS
AATGCACGCCACATGACAAG+CGG 0.617629 5.1:+60875857 None:intergenic
TTTACGGTTGCGTTTAACGG+TGG 0.618613 5.1:+60877632 None:intergenic
TCAAAGAAATGACAAAGGAA+GGG 0.618886 5.1:-60877284 MS.gene78516:CDS
AATTTAACAGTGGATTGAAG+AGG 0.619121 5.1:-60877329 MS.gene78516:intron
CAGAACAAACGCGACGAAGG+AGG 0.619183 5.1:-60877682 MS.gene78516:CDS
GGCACCTTAAGCAAGACAAG+AGG 0.620603 5.1:-60874614 MS.gene78516:CDS
GGGTGGCATGCAGATAGTAA+TGG 0.625312 5.1:-60876256 MS.gene78516:CDS
CAATTAAGAAATTGACCCAA+AGG 0.625816 5.1:-60874635 MS.gene78516:CDS
TGGCCAATCTTCTGGCTCAG+AGG 0.629159 5.1:-60874514 MS.gene78516:CDS
AATGGAACTTCAAGATGCAG+AGG 0.629339 5.1:-60876113 MS.gene78516:CDS
CCGAACTTGAGATACGTACA+TGG 0.632394 5.1:-60876288 MS.gene78516:CDS
CCATTGCTCTCCTTTAGAGG+AGG 0.635145 5.1:-60874340 MS.gene78516:intron
CTACATGGTGATAATCAGGA+TGG 0.636072 5.1:-60874588 MS.gene78516:CDS
CTTCAGATATTCCTGCAATG+CGG 0.636107 5.1:+60874389 None:intergenic
TATATACACTATGGTTGATG+TGG 0.641942 5.1:-60870946 MS.gene78516:CDS
TGTGGTAATTGCAATTGCAA+AGG 0.644805 5.1:-60877079 MS.gene78516:CDS
CCTCCTCTAAAGGAGAGCAA+TGG 0.645248 5.1:+60874340 None:intergenic
CGTTGTGGCCAACTTCTAGG+TGG 0.649865 5.1:-60876412 MS.gene78516:CDS
GAGGCATGTCCTGTATGTTG+TGG 0.650351 5.1:-60877097 MS.gene78516:CDS
CGGGTACACAAATTGTCCCA+AGG 0.655726 5.1:-60875470 MS.gene78516:CDS
TTTGTTTCTCCTGAAAACGT+TGG 0.656335 5.1:-60871643 MS.gene78516:CDS
TTATCTGCATGGTGGAAAGG+CGG 0.658125 5.1:-60876347 MS.gene78516:CDS
GTTGGCCACAACGAAGCTCG+CGG 0.661562 5.1:+60876422 None:intergenic
CGCGGCAACAGAGGACACAA+AGG 0.661878 5.1:+60876440 None:intergenic
TTAGATGCACCACATGCCAA+CGG 0.661969 5.1:-60877258 MS.gene78516:CDS
TGATCCAATTGTATTAAATG+TGG 0.662345 5.1:+60876861 None:intergenic
TGACATACACAATAACTCAA+GGG 0.663436 5.1:-60876041 MS.gene78516:CDS
TGAGTGCTTCCGTTTGACAG+AGG 0.665002 5.1:-60871620 MS.gene78516:CDS
TTCTGGCTCAGAGGTCAAGG+AGG 0.666246 5.1:-60874505 MS.gene78516:CDS
AATGTTCTAAAGCCAGACAT+GGG 0.666543 5.1:-60875280 MS.gene78516:CDS
GGAAGAAGATCAAATTGCTG+AGG 0.669254 5.1:-60877116 MS.gene78516:CDS
TCAAGTTCGGAACCAGCATG+AGG 0.678381 5.1:+60876301 None:intergenic
TGGCAAGGAAGATATGAACT+CGG 0.681914 5.1:+60875363 None:intergenic
GCTCTTTGCGTAGATTCCAG+GGG 0.682462 5.1:-60876013 MS.gene78516:CDS
GGCAGTAGATCTTCATCACG+AGG 0.683005 5.1:-60875969 MS.gene78516:CDS
CAATGTCAAAGAAATGACAA+AGG 0.691997 5.1:-60877289 MS.gene78516:CDS
GGCCAAGATTATCTGCATGG+TGG 0.693766 5.1:-60876355 MS.gene78516:CDS
TGAATGCTTAGATTGGTGCG+AGG 0.693830 5.1:-60875783 MS.gene78516:intron
ATGAACTCGGCACAATGACG+AGG 0.695465 5.1:+60875376 None:intergenic
AGAAGACGACACATTGACGA+CGG 0.696403 5.1:-60877787 MS.gene78516:CDS
CTGCATCAGACATATCACAA+TGG 0.714139 5.1:+60875191 None:intergenic
GTACATGGTCAAAATCTGGG+TGG 0.756530 5.1:-60876273 MS.gene78516:CDS
TCAATGGCACTGGAGCAGAG+GGG 0.763341 5.1:-60875933 MS.gene78516:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.1 gene 60870917 60877823 60870917 ID=MS.gene78516
chr5.1 mRNA 60870917 60877823 60870917 ID=MS.gene78516.t1;Parent=MS.gene78516
chr5.1 exon 60877446 60877823 60877446 ID=MS.gene78516.t1.exon1;Parent=MS.gene78516.t1
chr5.1 CDS 60877446 60877823 60877446 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60877226 60877341 60877226 ID=MS.gene78516.t1.exon2;Parent=MS.gene78516.t1
chr5.1 CDS 60877226 60877341 60877226 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60877051 60877150 60877051 ID=MS.gene78516.t1.exon3;Parent=MS.gene78516.t1
chr5.1 CDS 60877051 60877150 60877051 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60876820 60876964 60876820 ID=MS.gene78516.t1.exon4;Parent=MS.gene78516.t1
chr5.1 CDS 60876820 60876964 60876820 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60876616 60876679 60876616 ID=MS.gene78516.t1.exon5;Parent=MS.gene78516.t1
chr5.1 CDS 60876616 60876679 60876616 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60875784 60876522 60875784 ID=MS.gene78516.t1.exon6;Parent=MS.gene78516.t1
chr5.1 CDS 60875784 60876522 60875784 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60875192 60875521 60875192 ID=MS.gene78516.t1.exon7;Parent=MS.gene78516.t1
chr5.1 CDS 60875192 60875521 60875192 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60874341 60874712 60874341 ID=MS.gene78516.t1.exon8;Parent=MS.gene78516.t1
chr5.1 CDS 60874341 60874712 60874341 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60873045 60873117 60873045 ID=MS.gene78516.t1.exon9;Parent=MS.gene78516.t1
chr5.1 CDS 60873045 60873117 60873045 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60872858 60872943 60872858 ID=MS.gene78516.t1.exon10;Parent=MS.gene78516.t1
chr5.1 CDS 60872858 60872943 60872858 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60871570 60871689 60871570 ID=MS.gene78516.t1.exon11;Parent=MS.gene78516.t1
chr5.1 CDS 60871570 60871689 60871570 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
chr5.1 exon 60870917 60870982 60870917 ID=MS.gene78516.t1.exon12;Parent=MS.gene78516.t1
chr5.1 CDS 60870917 60870982 60870917 ID=cds.MS.gene78516.t1;Parent=MS.gene78516.t1
Gene Sequence

>MS.gene78516

ATGGCGTTGGCGGGAGAAGACGACACATTGACGACGGCGTCATCGTTGAACGGTTCATGCGACGCCTTGGCTGCGACTGAGAGACCATCTATCCTTCATCGCTATTCTAGGAAGAAACGCAGAACAAACGCGACGAAGGAGGATTTAACCAAGAACGTAGAACCACCAACCACCGTTAAACGCAACCGTAAAACGGAAGAAGGTGCTTCTTCTACTACCGTTGCTGCTGTTGTTGTTCGAAAGAGTGAACGGATAAGAAAAACAAACACATCTTATCATGCTGGTTTAGAGCTTCAAGAAAAACCTAAAAAAGGAAAACAGAGTCATCAAAATCATACGAAAGCTGAAAAAATCAACAACACAATTCGTCGTAATAAGGTCACAACGTCTTCTCTTCTTCTTCTAATGATTACTACTATTATTATTGTTACATTCCTTTGTGGTTCTGTAATTTACTAAATCTGATTCAACAAATTTAACAGTGGATTGAAGAGGAGTCTTTAATGTGCCATCAATGTCAAAGAAATGACAAAGGAAGGGTTGTTAGATGCACCACATGCCAACGGAAAAGATTTTGTGTATGCTGTATTAACAAATGGTATGTATCTATATTTCTACAACTTCTTCATTTGCCAATGTAATGTATCATATATATATTGTTTTTGTTCTTTAGGTATCCTCACTTGGAAGAAGATCAAATTGCTGAGGCATGTCCTGTATGTTGTGGTAATTGCAATTGCAAAGGGTGTTTGAGATCACGGAAACTTATCGATGTATGTCTCTAACTTCTTCGACCCCTTTTACGTACATTTTCTCTATTCATTCAACATTTTTAATCTTATTATCTTATACATTACAGTCAATTAAACAAAAAGAGGACGACGATACCAACAATGATCATCAGGTTGAGGTCTCCAAGTATATGCTCAAAGCTCTTCTACCACATTTAATACAATTGGATCAACAACAAATGTCTGAGAAGGAGATAGAAGCCAAGATACAAGGTATTTTATATACACCTTTCTTTCTTTTCAATTACTTACATACTAATTCATTTCCCACATGTGCTTTTTCATGGTCGATCATTTTTAGTTTCATATATTTCCTCTTTTTTTTTAATTTGCTTTTGGGATCAACATTGTAGGACGCTCACTCTCTGAGCTAAAAATTAAAGTATCAGTTCTCCATAATGATGAGCGCGCGTACTGGTAGGGAATTATTTCGTATATAAATTGGATTTTTACGTAGTTAGTTAGTTATTCCAATCCTATGTTGTTATTAATGACTCATTACTTTTGTAGTGACAACTGCAAAACATCAATATTTGACTACCACAGAAGATGCACAAAATGCTCTTTTGACCTTTGTGTCCTCTGTTGCCGCGAGCTTCGTTGTGGCCAACTTCTAGGTGGTGCAGACCCATTTGATTTTGAGTTTATCTTCCGAGGCCAAGATTATCTGCATGGTGGAAAGGCGGAGGAAGAAACCAGATACAAAGCACCTCATGCTGGTTCCGAACTTGAGATACGTACATGGTCAAAATCTGGGTGGCATGCAGATAGTAATGGCAATATTCCCTGTCCAAAATCTAAGAATAAATGTGATCATGGTTACCTTGAACTGAGAAGCATATTTCAGCCAAATTACATCTCTGACTTAGTATGTAAAGCAAAAGAACTTGCAGATTCAATGGAACTTCAAGATGCAGAGGAGACTCTTGATAATAGCTGCTCTTGTTTGAAGCCTGTTAGGAACAGAGATGACATACACAATAACTCAAGGGAAGCAGCTCTTTGCGTAGATTCCAGGGGCAATTTTTTGTACCATCCTAGGGCAGTAGATCTTCATCACGAGGATTTAGGACATTTTCAATGGCACTGGAGCAGAGGGGAGCCTGTAATTGTCAGCAATGTACTTGAATGTACATCTGGTTTAAGCTGGGAGCCGCTTGTCATGTGGCGTGCATTTCGTCAGAAAAGCAAGACCAAGCACAAGCCACTTTTGGATGTTAAGGCGCTTGAATGCTTAGATTGGTGCGAGGTTTGTTCGATTTCTAAACCTTGTACATGAATTTTGTTCGATTTCTTGATCTTTGAGCTTCTCATATGGTTTCTTGCACTCTAGTGTCCCTTGCATCCCCTGCTTTCTGTTTTTCTGTTGGTATTTTTCTCTGTTTCTTATGCATACTAACCGCAGTTGTTTTCTTTTTCTTTCATTTCTGTATTTTTTTTTTTTACTATACTTGACATGTCTATGTGTTACCGGTTATTTAAATTTTATTTGAGTTTTTGATTCTATGCAGATAGATATTAATGTGCACCAATTCTTTACCGGGTACACAAATTGTCCCAAGGATAAACAGGATTGGCCTCGAGTATTGAAATTGAAAGATTGGCCTCCTTCTAATTTATTTGAGGAAAGTTTACCTCGTCATTGTGCCGAGTTCATATCTTCCTTGCCATTTAAAGAATATACAAATCCTTTCAAAGGTGCTCTCAACCTTGCTGTGAAGCTGCCTGATAATGTTCTAAAGCCAGACATGGGACCAAAAACATATATAGCTTATGGATTTTCTCAGGAGCTTGGACGGGGTGATTCAGTGACCAAGCTCCATTGTGATATGTCTGATGCAGTATGCTTCAATTCTCTGTGTCTTTCCTGGACTGTTTTTTGTAGATTTTGTTATGAATTTTTTTATGTTTATATTTTTATTTATTATTTGAGTATCATATTGAAAGATGCATAATTTATAGGATGTAAATGATGCCTTTGTATGCCATTGCATTAAGAGCTGATAGTTGTGTAGTTTTAAGTACATCAAATCTTAAATTTTTGGTCATTGATCTGTCTTTGAAACACTCATTTGATTATATATTTGATATATTTGATTTGAAGTGTCATTGACCTTGGTATTTTGGTAACTCATAATTTGAAAGTATTATTTTTCTGATTAGTTGCATCATTTGACAAAGTATTTTTTAGACTTATATGCACTGTCTTTCCCGATTAATTATTTGTTTGTACTTTATATGTTTTGCTTTGTGTCTTGAGTTCTTACTGTATTATGGGGAAAATTAAATTGTTTTGTTTCTTTTACAATTTGGCTTTTAGGTAAATGTGTTGACTCATATTGCTAAAGCAAAATTGGATCCTGCGAGTATTGATGCAATTAAGAAATTGACCCAAAGGCACCTTAAGCAAGACAAGAGGGAGCTACATGGTGATAATCAGGATGGAGAGACCAATTTGTCTTCTTCCATAAATGCTTCAGAGAGCACTGATGATGATGGCCAATCTTCTGGCTCAGAGGTCAAGGAGGTTGATAAAGCGACGATGAAACAAGAAAATGGTTTGTTGGTTGGAGATTCTTTAGATGGTGCTCTCTGGGATATTTTTCGGAGAGAGGACGTACCCGCATTGCAGGAATATCTGAAGAAGCATTTCAAAGAGTTCAGGCACGTCCATTGCTCTCCTTTAGAGGAGGTAAAGTTAGTTGATATGATTTTCTATTATCTGTTTTGATGCCGCTTGTTAACTTGAGTCATTAATGAAACTTAGTTGCTACTAATTAGTTTTGCTGCTGCGAACAAACATGCCATATAGCTACCCCTTTATTTTTCACATTTTGCTGCTGCGAACATGATAAAATTGACACTATCTCATTGCTTTTCCCTATTTGACCTAATTCTTAGCTTATGCTTTATTTTATTTTTCAGTGTTGTTACTGTTGTTTTGCTTATATACCTTGTAATATGTTCTATGGGGCCTTCATAGTTTAGTCTCATAGCCCATTTAACTCCCTATAAATTCCTATCAATAATTTGAGTTACATCAAGTAGTTTAGTTTTCACCTTTTCAAACAAAAGAACCAAAATGCCCCAAATAAAGGTATCGTGCAGCGTGGATTTGGCTTCGTCTGGGCATCACATTGCCTATTGCCTGCTGGCTGCTGCTACCTCGCCTCCTGTGGCGTTGCTTCTTTTTACCTAGCCCGCTCTTTTTGTAGAGACCTTGTGCCTCTCTTCTTTTTTCGTCTACCTAAATCTCTCTTTTTATAGTGTTATGCTTGTTATACTTCTGTACATTGTAATAAGTTGGATTGGTCCTTTGGTTGTTCAGTCCTTCAGCACATTGGTAAGTGCTGCCCAGTGTAAAAGGTTCCAGCACACTTTGTTAGGGGCAACTGTCCATAACAGATTGCAATTCCTATAAATCATTATCGTACTTTCAGTTTTGCGAATAAGCTACATCAACAGAAAGTTATCTCCAGTTATCTGTTTACATTTTCCTTCATTATCTCTTGTTTTCTCAGCTAGTGATTGCTAACATCTGTTAGGTTCAGATTTTTTTAATTATGAAATTTACATGTCCCAAAGGATTGGGCTTGGCGAAGGATCAAGCTGGTAATAAATAATATTTGTGAGTAGTTAATTTTGAATACATACTATCACGAGTAAGGAATTATGTTGATGTGAATAGCGGTATTGAAACATAAGATATGCAGGGAAATGCATTCTGGATGTAATTCCAGTCCAGTAGGTCACTGATTCAGTAGACGTTCTGTATATCTTTCGTTTTACGGTTTCAGTTTCGAAGTTCACTAGGCAGTAGACGGGGGCTTCTTTATTTAGATAACTCGGCAGTCTGGTGACCTTTCACTCCTTATGTATGTATCTTCTTGAATGACATCTCCTTTGGCAATGTAGGTTATTCACCCCATCCATGATCAGACCTTTTATCTAACCGTCCGGCATAAGAAAAAGCTAAAGGAGGAGTATGGTTAGTAGTGCTTATATTTTACTTATGTTGTTTGGTCTGTGGAGTGTAGATCAATAGGCTTGTACCATCTTGTTAACAATTCAACACTGACTTGTATGTAGGAATTGAGCCTTGGACTTTTGTTCAGAAGCTAGGGGATGCTGTTTTCATTCCAGCTGGTTGCCCCCACCAAGTCAGAAATTTGAAGGTAAAATTTACGTACATAGTTGTTTTGTTTTATGTTTAATGAATGCGTAAATTTTCCTTGTTTGCTAAGAAGTGTAGATGTTGATGTTATGAGTTTTACATAATAGAAGAGAAAAAAATAAATATAAATATGGTTAAAGATGATTGATAATGACTTGATGACTAACTATTATGCTACCAAGTTCCTGAAAGTTTGAGAATGTCCTAGTAGTTATATTGTATTCAAAATCCTTATCACATTAATGCTAAATAATTGTCATTTGACAATAATGTGATATTAACTTGATACAAAAGACTAAATGCTAATTCCTAGGTAAAACATTATTACAACAACGTCATAGGTAAAAAATACCTTCCATATTCTGTTATACTTCTTATTATAGAACATTATAATAGGAGATTGCCCAGTTAGTTATTATGCAATTATGTTTTTATTATTATCTGCGTGTGTCGTTTATGTATAGTAGTTATGAGTGTTTCTGTTTAGAATAAAGTAAGAAGGACATAATAGAATTGGTTATATGGAAATTAATTAAGTGGTTATTGGGACTCAAAATCACGGGGAACCAGCATAACCCATCACTGTCAAGTGTGTTCCTGTTCTGTCTTTTCTTGTAACAGTCTGTGTAAAAAATGTATATGTATATGTATTTGTGTGAATCTCTTCTCCAGAGGTTAGATCAATAATATTCCACTGTATTTCATACATAATTATACTTGCTACCAGTTTCTATCATATTCCCATTTAAACATTCCTAAGAGGGGGAGGGGTTTGGGAAAATGGTTCCTCTGAATGTGGGCATGGAATTTAGATTGAGCCTAACTCAATTTGCAAAAAACTAGCTTATAAGTGAGAGATACTTACCACATAAATACATCAAGCCATATCATCCAGCCGTCTAGATCGTTACTCGAGTTGCATGACAATTGTCGCCCAATGAATATAGCATAGCTTCTGATATCTTAGTTTTTAGGTTGGTCTTAACTCAAACCAATGAGTCATTTAAGTTAACGGTTTGAGTGCATCTAAGTTCTTTTTGAAGACTGCATTTTTAAACCTTCAATTTCTTACAGGTTACCCAAGCCATTATCGCTAGCTCACTTTAATGTTCTTGTTAATGCTTGAAAAATTTGTATGAACGTTAATGATATGCATCGTCATTTTTCAGTCATGCACCAAGGTTGCACTTGATTTTGTTTCTCCTGAAAACGTTGGTGAGTGCTTCCGTTTGACAGAGGAATTCCGCAAACTTCCAGTAAATCACAGGTCCATAGAGGACAAATTGGAGGTATGCCAGACTATTATGGATTTAATTCTTCTGTTTATACGCTTACCAAAGCAATTAAGCCTGATTAGAAGCTTTCAGTTAGACCTATTGTTGGCACTGTCATGTTGCAATTGGCCAAATCTGAACACTTCCATTGGTTGAACTGGTTCAAGCATATTATTCACATTTGTTTGAATTGATCATTTTTTACCATCTAGATTCTAGACTGCACAGTGCTATTTAATTCACTAGTAATTATAATGTAACATTAATTTCTGAAAAAATGATGGGAATAGGAAAAAATGGTTATGCATAGTTAGTAGACAATTTAACTTTTTATCGAATGTTTCTTGGAAAAGTCATCAATAGCTCTAGTGAAGAGTATTTGTTTTATATTTTATATTTTTCTATCTATTAGTATTGTAGTTTTTATGCAATGACAAGGTTCTAATATTAACTATTTATACTTGTTGAATGTTTGGCAGTTTTGAGTGTAAAGCTATTTCAACAAACTATGTTACCGTGTCACACCGTTATGTCATATCAATCGATTCTTTATAGTATTTTCTGTAAAGTTTGTTCCTAATTGCTGCTTCTTTTGGTGACAGGTGAAGAAAATGATTATATACACTATGGTTGATGTGGTCAAAACATTAGAGAAAGCATTGGTATGA
Protein sequence

>MS.gene78516.t1

MALAGEDDTLTTASSLNGSCDALAATERPSILHRYSRKKRRTNATKEDLTKNVEPPTTVKRNRKTEEGASSTTVAAVVVRKSERIRKTNTSYHAGLELQEKPKKGKQSHQNHTKAEKINNTIRRNKWIEEESLMCHQCQRNDKGRVVRCTTCQRKRFCVCCINKWYPHLEEDQIAEACPVCCGNCNCKGCLRSRKLIDSIKQKEDDDTNNDHQVEVSKYMLKALLPHLIQLDQQQMSEKEIEAKIQGRSLSELKIKVSVLHNDERAYCDNCKTSIFDYHRRCTKCSFDLCVLCCRELRCGQLLGGADPFDFEFIFRGQDYLHGGKAEEETRYKAPHAGSELEIRTWSKSGWHADSNGNIPCPKSKNKCDHGYLELRSIFQPNYISDLVCKAKELADSMELQDAEETLDNSCSCLKPVRNRDDIHNNSREAALCVDSRGNFLYHPRAVDLHHEDLGHFQWHWSRGEPVIVSNVLECTSGLSWEPLVMWRAFRQKSKTKHKPLLDVKALECLDWCEIDINVHQFFTGYTNCPKDKQDWPRVLKLKDWPPSNLFEESLPRHCAEFISSLPFKEYTNPFKGALNLAVKLPDNVLKPDMGPKTYIAYGFSQELGRGDSVTKLHCDMSDAVNVLTHIAKAKLDPASIDAIKKLTQRHLKQDKRELHGDNQDGETNLSSSINASESTDDDGQSSGSEVKEVDKATMKQENGLLVGDSLDGALWDIFRREDVPALQEYLKKHFKEFRHVHCSPLEEVIHPIHDQTFYLTVRHKKKLKEEYGIEPWTFVQKLGDAVFIPAGCPHQVRNLKSCTKVALDFVSPENVGECFRLTEEFRKLPVNHRSIEDKLEVKKMIIYTMVDVVKTLEKALV