AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78804

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78804.t1 MTR_4g021760 84.713 314 45 2 1 313 1 312 0.0 546
MS.gene78804.t1 MTR_4g021855 66.776 304 89 5 1 295 1 301 2.42e-139 396
MS.gene78804.t1 MTR_4g021845 85.845 219 29 1 1 219 1 217 7.74e-138 389
MS.gene78804.t1 MTR_4g021790 76.033 242 56 1 1 242 1 240 1.73e-129 370
MS.gene78804.t1 MTR_5g055260 60.173 231 63 5 1 231 1 202 2.51e-86 259
MS.gene78804.t1 MTR_4g023980 78.417 139 28 1 55 193 35 171 9.13e-74 225
MS.gene78804.t1 MTR_6g045327 45.752 306 143 9 1 296 1 293 3.25e-71 225
MS.gene78804.t1 MTR_1g032570 37.808 365 160 9 10 309 11 373 1.58e-68 225
MS.gene78804.t1 MTR_3g086100 39.921 253 119 5 10 231 13 263 8.88e-57 192
MS.gene78804.t1 MTR_8g079940 39.695 262 125 6 9 239 24 283 5.15e-56 190
MS.gene78804.t1 MTR_4g121020 38.868 265 133 4 10 248 31 292 6.00e-55 189
MS.gene78804.t1 MTR_2g034960 39.245 265 133 4 10 248 29 291 6.05e-55 188
MS.gene78804.t1 MTR_4g121020 38.868 265 133 4 10 248 31 292 6.11e-55 189
MS.gene78804.t1 MTR_8g105600 40.323 248 126 3 4 231 3 248 1.58e-53 184
MS.gene78804.t1 MTR_1g013170 39.200 250 118 4 7 226 2 247 2.32e-50 174
MS.gene78804.t1 MTR_4g131600 36.032 247 137 4 9 234 19 265 1.86e-48 171
MS.gene78804.t1 MTR_7g117705 36.090 266 142 4 7 244 2 267 2.79e-48 167
MS.gene78804.t1 MTR_8g093040 42.797 236 112 6 1 231 1 218 6.60e-48 162
MS.gene78804.t1 MTR_3g106220 37.652 247 124 3 10 226 8 254 1.96e-46 166
MS.gene78804.t1 MTR_1g013180 36.585 246 130 3 7 226 153 398 3.18e-44 160
MS.gene78804.t1 MTR_1g032570 33.987 306 136 8 68 309 1 304 1.65e-43 157
MS.gene78804.t1 MTR_4g131570 32.800 250 142 4 10 233 17 266 1.47e-42 155
MS.gene78804.t1 MTR_2g450070 34.510 255 132 4 10 229 18 272 2.73e-42 155
MS.gene78804.t1 MTR_4g131580 33.065 248 143 4 9 233 16 263 3.58e-42 154
MS.gene78804.t1 MTR_2g450070 34.510 255 132 4 10 229 18 272 3.60e-42 154
MS.gene78804.t1 MTR_3g102590 38.140 215 104 4 10 222 8 195 4.25e-41 143
MS.gene78804.t1 MTR_6g016850 43.750 160 86 2 4 161 3 160 4.75e-41 141
MS.gene78804.t1 MTR_3g102600 37.156 218 99 3 10 226 8 188 1.47e-39 139
MS.gene78804.t1 MTR_3g102590 37.264 212 104 4 10 219 8 192 1.13e-38 135
MS.gene78804.t1 MTR_3g086100 39.175 194 86 4 68 231 1 192 1.95e-38 142
MS.gene78804.t1 MTR_3g102600 35.616 219 101 4 4 221 4 183 4.78e-37 131
MS.gene78804.t1 MTR_0450s0040 29.719 249 154 4 12 248 6 245 4.37e-32 122
MS.gene78804.t1 MTR_3g102600 36.416 173 73 2 54 226 4 139 5.61e-32 117
MS.gene78804.t1 MTR_7g026400 33.175 211 120 4 52 248 30 233 1.26e-31 124
MS.gene78804.t1 MTR_3g102600 34.524 168 73 2 54 221 4 134 2.04e-29 109
MS.gene78804.t1 MTR_4g121020 36.559 186 90 3 88 248 6 188 1.38e-28 115
MS.gene78804.t1 MTR_8g032710 23.810 357 212 10 12 309 19 374 2.96e-21 94.0
MS.gene78804.t1 MTR_1g013160 34.211 152 67 3 71 193 1 148 5.50e-19 82.8
MS.gene78804.t1 MTR_2g090675 38.298 94 55 2 135 226 125 217 3.79e-14 72.8
MS.gene78804.t1 MTR_5g041350 55.556 54 23 1 170 222 15 68 1.31e-13 70.9
MS.gene78804.t1 MTR_6g032990 44.872 78 42 1 170 246 42 119 3.84e-13 69.7
MS.gene78804.t1 MTR_7g098250 43.678 87 44 3 161 245 253 336 4.38e-13 69.7
MS.gene78804.t1 MTR_4g078810 48.276 58 30 0 169 226 157 214 6.52e-13 69.3
MS.gene78804.t1 MTR_7g088070 40.659 91 51 2 168 257 48 136 8.82e-13 67.8
MS.gene78804.t1 MTR_4g081710 51.852 54 25 1 170 222 17 70 1.60e-12 67.8
MS.gene78804.t1 MTR_2g086450 53.704 54 24 1 170 222 45 98 2.48e-12 67.4
MS.gene78804.t1 MTR_7g088070 51.786 56 26 1 168 222 48 103 2.95e-12 66.2
MS.gene78804.t1 MTR_5g014040 36.250 80 51 0 169 248 310 389 4.10e-12 67.0
MS.gene78804.t1 MTR_7g115530 47.222 72 36 2 170 239 218 289 5.22e-12 65.9
MS.gene78804.t1 MTR_7g115530 40.659 91 51 3 170 258 218 307 6.90e-12 65.5
MS.gene78804.t1 MTR_7g115530 50.000 64 30 2 170 231 218 281 1.20e-11 65.1
MS.gene78804.t1 MTR_2g027860 55.556 54 23 1 170 222 49 102 1.34e-11 64.7
MS.gene78804.t1 MTR_7g115530 40.659 91 51 3 170 258 218 307 1.63e-11 65.1
MS.gene78804.t1 MTR_7g089010 48.276 58 30 0 169 226 90 147 1.79e-11 64.3
MS.gene78804.t1 MTR_4g098870 48.148 54 28 0 169 222 309 362 1.88e-11 65.1
MS.gene78804.t1 MTR_4g098870 48.148 54 28 0 169 222 309 362 1.90e-11 65.1
MS.gene78804.t1 MTR_4g098870 48.148 54 28 0 169 222 329 382 2.00e-11 65.1
MS.gene78804.t1 MTR_4g098870 48.148 54 28 0 169 222 348 401 2.18e-11 65.1
MS.gene78804.t1 MTR_4g098870 48.148 54 28 0 169 222 329 382 2.18e-11 64.7
MS.gene78804.t1 MTR_1g080330 42.857 84 45 3 161 242 165 247 2.78e-11 63.5
MS.gene78804.t1 MTR_7g093030 42.466 73 40 2 167 238 209 280 3.15e-11 63.5
MS.gene78804.t1 MTR_1g093080 45.122 82 35 3 151 229 199 273 3.21e-11 63.9
MS.gene78804.t1 MTR_4g064730 41.250 80 45 2 147 226 110 187 3.72e-11 63.5
MS.gene78804.t1 MTR_1g080330 47.059 68 34 2 166 231 171 238 6.58e-11 63.2
MS.gene78804.t1 MTR_7g093030 47.368 57 29 1 167 222 209 265 8.40e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78804.t1 AT2G01760 38.403 263 121 5 7 232 4 262 1.47e-55 185
MS.gene78804.t1 AT1G67710 40.075 267 124 8 11 243 8 272 2.25e-53 182
MS.gene78804.t1 AT3G16857 36.039 308 139 9 10 259 33 340 1.28e-50 178
MS.gene78804.t1 AT3G16857 36.688 308 137 11 10 259 33 340 1.31e-50 177
MS.gene78804.t1 AT2G01760 35.052 291 120 7 7 232 4 290 1.31e-49 170
MS.gene78804.t1 AT4G31920 36.481 233 138 2 10 232 13 245 3.86e-46 164
MS.gene78804.t1 AT2G25180 38.750 240 123 6 10 226 13 251 1.04e-43 158
MS.gene78804.t1 AT3G16857 34.944 269 117 9 49 259 2 270 2.06e-39 146
MS.gene78804.t1 AT5G58080 37.008 254 126 7 10 236 14 260 3.80e-39 145
MS.gene78804.t1 AT1G67710 40.191 209 90 7 68 243 1 207 6.93e-36 134
MS.gene78804.t1 AT2G01760 35.821 201 89 4 68 232 1 197 4.07e-35 129
MS.gene78804.t1 AT5G49240 31.356 236 137 5 9 219 37 272 3.07e-33 124
MS.gene78804.t1 AT3G62670 30.258 271 159 9 28 272 53 319 9.35e-32 123
MS.gene78804.t1 AT1G49190 27.734 256 154 6 10 234 30 285 1.44e-28 114
MS.gene78804.t1 AT1G67710 38.624 189 81 7 88 243 12 198 8.11e-27 109
MS.gene78804.t1 AT5G07210 27.778 270 141 7 14 231 16 283 3.90e-25 105
MS.gene78804.t1 AT5G07210 26.978 278 141 8 14 231 16 291 1.46e-22 98.6
MS.gene78804.t1 AT2G27070 25.379 264 140 8 14 222 16 277 1.87e-19 89.4
MS.gene78804.t1 AT1G49190 32.258 124 83 1 10 132 30 153 1.42e-17 83.6
MS.gene78804.t1 AT5G29000 40.566 106 54 4 135 238 203 301 1.26e-15 76.6
MS.gene78804.t1 AT5G29000 38.938 113 60 4 135 245 203 308 1.41e-15 77.0
MS.gene78804.t1 AT5G29000 38.938 113 60 4 135 245 160 265 1.53e-15 76.6
MS.gene78804.t1 AT5G29000 38.938 113 60 4 135 245 160 265 1.53e-15 76.6
MS.gene78804.t1 AT5G44190 48.333 60 31 0 167 226 144 203 1.29e-13 71.2
MS.gene78804.t1 AT5G44190 48.333 60 31 0 167 226 144 203 1.49e-13 70.9
MS.gene78804.t1 AT3G04030 55.556 54 23 1 170 222 45 98 3.86e-13 69.7
MS.gene78804.t1 AT3G04030 55.556 54 23 1 170 222 45 98 4.36e-13 69.7
MS.gene78804.t1 AT4G28610 47.126 87 40 4 170 252 225 309 7.19e-13 68.9
MS.gene78804.t1 AT5G18240 55.556 54 23 1 170 222 45 98 8.09e-13 68.9
MS.gene78804.t1 AT5G18240 55.556 54 23 1 170 222 45 98 8.09e-13 68.9
MS.gene78804.t1 AT5G18240 55.556 54 23 1 170 222 45 98 8.95e-13 68.9
MS.gene78804.t1 AT5G18240 55.556 54 23 1 170 222 45 98 8.95e-13 68.9
MS.gene78804.t1 AT5G18240 55.556 54 23 1 170 222 45 98 8.98e-13 68.9
MS.gene78804.t1 AT2G01060 45.783 83 42 2 159 238 4 86 1.13e-12 67.8
MS.gene78804.t1 AT5G59570 42.105 76 41 1 151 226 125 197 1.63e-12 67.4
MS.gene78804.t1 AT5G59570 42.105 76 41 1 151 226 125 197 1.63e-12 67.4
MS.gene78804.t1 AT3G04450 48.276 58 29 1 166 222 234 291 1.63e-12 67.4
MS.gene78804.t1 AT3G04030 52.830 53 25 0 170 222 45 97 2.61e-12 67.4
MS.gene78804.t1 AT3G46640 43.210 81 43 2 146 226 123 200 3.52e-12 66.6
MS.gene78804.t1 AT3G46640 43.210 81 43 2 146 226 123 200 3.52e-12 66.6
MS.gene78804.t1 AT3G46640 43.210 81 43 2 146 226 123 200 3.64e-12 66.6
MS.gene78804.t1 AT3G04450 48.276 58 29 1 166 222 234 291 3.71e-12 67.0
MS.gene78804.t1 AT3G04450 48.276 58 29 1 166 222 194 251 3.95e-12 67.0
MS.gene78804.t1 AT3G04450 48.276 58 29 1 166 222 194 251 3.95e-12 67.0
MS.gene78804.t1 AT1G69580 51.852 54 25 1 170 222 34 87 6.57e-12 65.9
MS.gene78804.t1 AT2G20570 46.552 58 31 0 169 226 152 209 6.66e-12 66.2
MS.gene78804.t1 AT1G69580 51.852 54 25 1 170 222 34 87 6.98e-12 65.9
MS.gene78804.t1 AT5G05090 42.697 89 39 3 138 226 61 137 7.19e-12 65.1
MS.gene78804.t1 AT2G40970 46.552 58 31 0 169 226 104 161 1.14e-11 64.3
MS.gene78804.t1 AT3G10760 48.276 58 30 0 169 226 104 161 1.34e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.84e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.84e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.84e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.84e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.84e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.84e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.97e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.97e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.97e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.97e-11 65.1
MS.gene78804.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.97e-11 65.1
MS.gene78804.t1 AT2G20570 47.273 55 29 0 172 226 171 225 4.83e-11 63.5
MS.gene78804.t1 AT3G24120 48.529 68 31 2 170 233 41 108 6.18e-11 62.8
MS.gene78804.t1 AT3G24120 48.529 68 31 2 170 233 41 108 6.22e-11 62.8
MS.gene78804.t1 AT4G04605 46.970 66 33 2 168 231 12 77 9.65e-11 61.2

Find 58 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATTGTGTCGCAGAATTC+AGG 0.268438 4.2:-80786139 None:intergenic
ACATATTGCCAGCCATTTAC+AGG 0.282271 4.2:+80785641 MS.gene78804:CDS
TTCAGTTACCAAATGTAGTA+TGG 0.293347 4.2:+80784916 MS.gene78804:intron
CAACGACATTGGGTGGAAAT+TGG 0.345706 4.2:+80786179 MS.gene78804:CDS
TGAGTTGTAGGGTAACATTT+TGG 0.376494 4.2:-80785033 None:intergenic
GATAAATCATCATCAACATA+TGG 0.380393 4.2:-80786251 None:intergenic
AATGTGGGGCTTGCGAATAT+TGG 0.385194 4.2:+80785229 MS.gene78804:CDS
CGAATATTGGACTAAGCCTT+TGG 0.392176 4.2:+80785242 MS.gene78804:CDS
TGACAGGAATGTTGAGTTGT+AGG 0.408636 4.2:-80785045 None:intergenic
ATCAGAAGCCATACTACATT+TGG 0.409595 4.2:-80784924 None:intergenic
AATAAAGAATATGAAATTGT+TGG 0.416766 4.2:+80785327 MS.gene78804:CDS
CTTTCTTTCCAACATGTTGA+AGG 0.419409 4.2:-80784948 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.435563 4.2:+80785418 MS.gene78804:CDS
AAAAGAAACACTTGCAAAGA+AGG 0.435703 4.2:+80785404 MS.gene78804:CDS
GGTTCAAGAAAATAGAAAAC+GGG 0.441425 4.2:+80785359 MS.gene78804:CDS
TTGCGATGCACAACGACATT+GGG 0.452490 4.2:+80786169 MS.gene78804:CDS
TTGAGACCGGCTGGGAATGA+TGG 0.453544 4.2:-80784720 None:intergenic
CTTCAACATGTTGGAAAGAA+AGG 0.459586 4.2:+80784949 MS.gene78804:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4.2:-80785061 None:intergenic
ACATCTCCTCAATAGAGTTA+AGG 0.467190 4.2:-80784773 None:intergenic
TTCTCGTGACCAAGACAAAC+GGG 0.467708 4.2:-80785427 None:intergenic
TCAACATATGGGAACAAGTT+GGG 0.468173 4.2:-80786239 None:intergenic
TACTCACTCAATGCCGGAGC+AGG 0.473606 4.2:+80786103 MS.gene78804:CDS
GCAATAACCTTGAGACCGGC+TGG 0.475576 4.2:-80784729 None:intergenic
AAAAGTGTTGCTCATAATGT+TGG 0.490442 4.2:-80786207 None:intergenic
AAGTCATAAACATCCATGTC+TGG 0.491105 4.2:-80785007 None:intergenic
TGGTTCAAGAAAATAGAAAA+CGG 0.501089 4.2:+80785358 MS.gene78804:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4.2:-80785488 None:intergenic
ATATGAAATTGTTGGAAGCT+CGG 0.515274 4.2:+80785335 MS.gene78804:CDS
ATTGCGATGCACAACGACAT+TGG 0.516098 4.2:+80786168 MS.gene78804:CDS
TGTAATCGATATCATTATCA+AGG 0.517908 4.2:+80784795 MS.gene78804:CDS
ATCATGAAATCTATTGAATG+TGG 0.519581 4.2:+80785213 MS.gene78804:CDS
GACAGGAATGTTGAGTTGTA+GGG 0.519779 4.2:-80785044 None:intergenic
TCATGAAATCTATTGAATGT+GGG 0.521948 4.2:+80785214 MS.gene78804:CDS
ATTCTCGTGACCAAGACAAA+CGG 0.526657 4.2:-80785428 None:intergenic
AAAATGAACCTGTAAATGGC+TGG 0.527802 4.2:-80785649 None:intergenic
TTCTGATGCCTTCAACATGT+TGG 0.527887 4.2:+80784940 MS.gene78804:CDS
CATGTTGCAAGAATTAGAAA+CGG 0.528421 4.2:+80785294 MS.gene78804:CDS
CACTCTCCTTAACTCTATTG+AGG 0.546467 4.2:+80784767 MS.gene78804:CDS
CTACGTGCAGTCAATCAACT+AGG 0.547867 4.2:+80785465 MS.gene78804:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4.2:-80786250 None:intergenic
AGCTCAACAGAAGAGACAAA+AGG 0.548661 4.2:+80786026 MS.gene78804:CDS
AGAAAACTCTGGTCCTGCTC+CGG 0.548712 4.2:-80786116 None:intergenic
ATCATTCCCAGCCGGTCTCA+AGG 0.552068 4.2:+80784722 MS.gene78804:CDS
ATCAACATATGGGAACAAGT+TGG 0.563419 4.2:-80786240 None:intergenic
CATGAAATCTATTGAATGTG+GGG 0.577494 4.2:+80785215 MS.gene78804:CDS
TGAGACCGGCTGGGAATGAT+GGG 0.577892 4.2:-80784719 None:intergenic
CGATGCACAACGACATTGGG+TGG 0.580466 4.2:+80786172 MS.gene78804:CDS
AATTGCAATAACCTTGAGAC+CGG 0.581075 4.2:-80784733 None:intergenic
AGAAGCTACTCACTCAATGC+CGG 0.591913 4.2:+80786097 MS.gene78804:CDS
CAGAATTCAGGAGAAAACTC+TGG 0.602905 4.2:-80786127 None:intergenic
CAATAACCTTGAGACCGGCT+GGG 0.613963 4.2:-80784728 None:intergenic
CAACAGAAGAGACAAAAGGT+AGG 0.617016 4.2:+80786030 MS.gene78804:CDS
GACAACCCATCATTCCCAGC+CGG 0.620153 4.2:+80784714 MS.gene78804:CDS
TGATGCTCATATGCCAGACA+TGG 0.629107 4.2:+80784994 MS.gene78804:CDS
TGAAATTGTTGGAAGCTCGG+TGG 0.670504 4.2:+80785338 MS.gene78804:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4.2:+80786264 MS.gene78804:CDS
GTTCAAGAAAATAGAAAACG+GGG 0.742093 4.2:+80785360 MS.gene78804:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG + chr4.2:80785327-80785346 MS.gene78804:CDS 15.0%
!!! AATATGTTCTTTTTTCATAT+CGG - chr4.2:80785628-80785647 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4.2:80785941-80785960 MS.gene78804:intron 15.0%
!! AAAAGATGAAAACTTGAATA+AGG - chr4.2:80784838-80784857 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4.2:80785064-80785083 None:intergenic 20.0%
!! AGCATAGTAAAAAAGTAATT+AGG - chr4.2:80785511-80785530 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4.2:80786253-80786272 None:intergenic 20.0%
!! CAATATAAGAATAAACAAAC+AGG - chr4.2:80784891-80784910 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4.2:80785596-80785615 MS.gene78804:CDS 20.0%
!!! ATATGTTCTTTTTTCATATC+GGG - chr4.2:80785627-80785646 None:intergenic 20.0%
!!! ATATTTCTTTTTTGTAGTGA+TGG + chr4.2:80785167-80785186 MS.gene78804:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4.2:80785597-80785616 None:intergenic 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4.2:80785564-80785583 MS.gene78804:intron 20.0%
!!! ATTCAAGTTTTCATCTTTTT+TGG + chr4.2:80784839-80784858 MS.gene78804:intron 20.0%
!!! TGTTTTTCTCTTAATTTTCA+GGG + chr4.2:80785565-80785584 MS.gene78804:intron 20.0%
!!! TTTTGCAGAAATATAGAAAT+CGG + chr4.2:80785997-80786016 MS.gene78804:intron 20.0%
! ATCATGAAATCTATTGAATG+TGG + chr4.2:80785213-80785232 MS.gene78804:CDS 25.0%
! GATAAATCATCATCAACATA+TGG - chr4.2:80786254-80786273 None:intergenic 25.0%
! TACAAAAATGAACCTGTAAA+TGG - chr4.2:80785656-80785675 None:intergenic 25.0%
! TCATGAAATCTATTGAATGT+GGG + chr4.2:80785214-80785233 MS.gene78804:CDS 25.0%
! TGGTTCAAGAAAATAGAAAA+CGG + chr4.2:80785358-80785377 MS.gene78804:CDS 25.0%
! TGTAATCGATATCATTATCA+AGG + chr4.2:80784795-80784814 MS.gene78804:CDS 25.0%
!! GTTTTTCTCTTAATTTTCAG+GGG + chr4.2:80785566-80785585 MS.gene78804:intron 25.0%
!! TTTCATGATAGAACTTTTTG+TGG - chr4.2:80785202-80785221 None:intergenic 25.0%
!!! AAGTCAAATTTTGTTTCAAG+AGG - chr4.2:80786077-80786096 None:intergenic 25.0%
AAAAGAAACACTTGCAAAGA+AGG + chr4.2:80785404-80785423 MS.gene78804:CDS 30.0%
AACTGAGAAACTATAGACTT+AGG - chr4.2:80785717-80785736 None:intergenic 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4.2:80785491-80785510 None:intergenic 30.0%
AGTAGCATATTATCAACAAG+AGG - chr4.2:80785910-80785929 None:intergenic 30.0%
ATATGAAATTGTTGGAAGCT+CGG + chr4.2:80785335-80785354 MS.gene78804:CDS 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4.2:80786264-80786283 MS.gene78804:CDS 30.0%
CATGAAATCTATTGAATGTG+GGG + chr4.2:80785215-80785234 MS.gene78804:CDS 30.0%
CATGTTGCAAGAATTAGAAA+CGG + chr4.2:80785294-80785313 MS.gene78804:CDS 30.0%
GGTTCAAGAAAATAGAAAAC+GGG + chr4.2:80785359-80785378 MS.gene78804:CDS 30.0%
GTTCAAGAAAATAGAAAACG+GGG + chr4.2:80785360-80785379 MS.gene78804:CDS 30.0%
TGATTTACAACAACAGACTT+AGG - chr4.2:80785748-80785767 None:intergenic 30.0%
TTCAGTTACCAAATGTAGTA+TGG + chr4.2:80784916-80784935 MS.gene78804:intron 30.0%
TTGAATAACATGCAAGACTA+AGG - chr4.2:80785786-80785805 None:intergenic 30.0%
!! AAAAGTGTTGCTCATAATGT+TGG - chr4.2:80786210-80786229 None:intergenic 30.0%
AAAAAGAGGTCAAGATCATG+TGG + chr4.2:80785268-80785287 MS.gene78804:CDS 35.0%
AAAATGAACCTGTAAATGGC+TGG - chr4.2:80785652-80785671 None:intergenic 35.0%
AAGTCATAAACATCCATGTC+TGG - chr4.2:80785010-80785029 None:intergenic 35.0%
AATTGCAATAACCTTGAGAC+CGG - chr4.2:80784736-80784755 None:intergenic 35.0%
ACATCTCCTCAATAGAGTTA+AGG - chr4.2:80784776-80784795 None:intergenic 35.0%
AGAGGATGTATAGAAGCTAA+TGG - chr4.2:80785892-80785911 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4.2:80785822-80785841 MS.gene78804:intron 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4.2:80786243-80786262 None:intergenic 35.0%
ATCAGAAGCCATACTACATT+TGG - chr4.2:80784927-80784946 None:intergenic 35.0%
CTTCAACATGTTGGAAAGAA+AGG + chr4.2:80784949-80784968 MS.gene78804:CDS 35.0%
CTTTCTTTCCAACATGTTGA+AGG - chr4.2:80784951-80784970 None:intergenic 35.0%
TAAGCCTTTGGTTGAAAAAG+AGG + chr4.2:80785254-80785273 MS.gene78804:CDS 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4.2:80786242-80786261 None:intergenic 35.0%
! CTTTGCAAGTGTTTCTTTTG+AGG - chr4.2:80785403-80785422 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4.2:80785036-80785055 None:intergenic 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4.2:80785261-80785280 None:intergenic 35.0%
ACATATTGCCAGCCATTTAC+AGG + chr4.2:80785641-80785660 MS.gene78804:CDS 40.0%
AGCTCAACAGAAGAGACAAA+AGG + chr4.2:80786026-80786045 MS.gene78804:CDS 40.0%
ATTCTCGTGACCAAGACAAA+CGG - chr4.2:80785431-80785450 None:intergenic 40.0%
CAACAGAAGAGACAAAAGGT+AGG + chr4.2:80786030-80786049 MS.gene78804:CDS 40.0%
CACTCTCCTTAACTCTATTG+AGG + chr4.2:80784767-80784786 MS.gene78804:CDS 40.0%
CAGAATTCAGGAGAAAACTC+TGG - chr4.2:80786130-80786149 None:intergenic 40.0%
CGAATATTGGACTAAGCCTT+TGG + chr4.2:80785242-80785261 MS.gene78804:CDS 40.0%
TTCTGATGCCTTCAACATGT+TGG + chr4.2:80784940-80784959 MS.gene78804:CDS 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4.2:80785047-80785066 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4.2:80785048-80785067 None:intergenic 40.0%
AATGTGGGGCTTGCGAATAT+TGG + chr4.2:80785229-80785248 MS.gene78804:CDS 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4.2:80786097-80786116 MS.gene78804:CDS 45.0%
ATTGCGATGCACAACGACAT+TGG + chr4.2:80786168-80786187 MS.gene78804:CDS 45.0%
CAACGACATTGGGTGGAAAT+TGG + chr4.2:80786179-80786198 MS.gene78804:CDS 45.0%
CTACGTGCAGTCAATCAACT+AGG + chr4.2:80785465-80785484 MS.gene78804:CDS 45.0%
GACATTGTGTCGCAGAATTC+AGG - chr4.2:80786142-80786161 None:intergenic 45.0%
TGAAATTGTTGGAAGCTCGG+TGG + chr4.2:80785338-80785357 MS.gene78804:CDS 45.0%
TGATGCTCATATGCCAGACA+TGG + chr4.2:80784994-80785013 MS.gene78804:CDS 45.0%
TTCTCGTGACCAAGACAAAC+GGG - chr4.2:80785430-80785449 None:intergenic 45.0%
TTGCGATGCACAACGACATT+GGG + chr4.2:80786169-80786188 MS.gene78804:CDS 45.0%
AGAAAACTCTGGTCCTGCTC+CGG - chr4.2:80786119-80786138 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4.2:80785418-80785437 MS.gene78804:CDS 50.0%
CAATAACCTTGAGACCGGCT+GGG - chr4.2:80784731-80784750 None:intergenic 50.0%
ATCATTCCCAGCCGGTCTCA+AGG + chr4.2:80784722-80784741 MS.gene78804:CDS 55.0%
CGATGCACAACGACATTGGG+TGG + chr4.2:80786172-80786191 MS.gene78804:CDS 55.0%
GACAACCCATCATTCCCAGC+CGG + chr4.2:80784714-80784733 MS.gene78804:CDS 55.0%
GCAATAACCTTGAGACCGGC+TGG - chr4.2:80784732-80784751 None:intergenic 55.0%
TACTCACTCAATGCCGGAGC+AGG + chr4.2:80786103-80786122 MS.gene78804:CDS 55.0%
TGAGACCGGCTGGGAATGAT+GGG - chr4.2:80784722-80784741 None:intergenic 55.0%
TTGAGACCGGCTGGGAATGA+TGG - chr4.2:80784723-80784742 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 80784699 80786289 80784699 ID=MS.gene78804
chr4.2 mRNA 80784699 80786289 80784699 ID=MS.gene78804.t1;Parent=MS.gene78804
chr4.2 exon 80784699 80784816 80784699 ID=MS.gene78804.t1.exon1;Parent=MS.gene78804.t1
chr4.2 CDS 80784699 80784816 80784699 ID=cds.MS.gene78804.t1;Parent=MS.gene78804.t1
chr4.2 exon 80784921 80785070 80784921 ID=MS.gene78804.t1.exon2;Parent=MS.gene78804.t1
chr4.2 CDS 80784921 80785070 80784921 ID=cds.MS.gene78804.t1;Parent=MS.gene78804.t1
chr4.2 exon 80785184 80785495 80785184 ID=MS.gene78804.t1.exon3;Parent=MS.gene78804.t1
chr4.2 CDS 80785184 80785495 80785184 ID=cds.MS.gene78804.t1;Parent=MS.gene78804.t1
chr4.2 exon 80785586 80785662 80785586 ID=MS.gene78804.t1.exon4;Parent=MS.gene78804.t1
chr4.2 CDS 80785586 80785662 80785586 ID=cds.MS.gene78804.t1;Parent=MS.gene78804.t1
chr4.2 exon 80786005 80786289 80786005 ID=MS.gene78804.t1.exon5;Parent=MS.gene78804.t1
chr4.2 CDS 80786005 80786289 80786005 ID=cds.MS.gene78804.t1;Parent=MS.gene78804.t1
Gene Sequence

>MS.gene78804

ATGGCAGTTTTCACTGACAACCCATCATTCCCAGCCGGTCTCAAGGTTATTGCAATTGATCATGACATCACTCTCCTTAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGGTTAGTTACTGAATCATACCTTATTCAAGTTTTCATCTTTTTTGGTAAAAATTCTTACTTTTATATGTATTCCTGTTTGTTTATTCTTATATTGTTTATTTCAGTTACCAAATGTAGTATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTAGTAAGAATTTATTCAACTTTTTCATTAAAATTCATTAGTTACACATTTCACATAATTTATTCATATATATGTACTTAAGAAGTGTGAATTGACATTATATTTCTTTTTTGTAGTGATGGCTGTTGATTCCACAAAAAGTTCTATCATGAAATCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAAGAGGTCAAGATCATGTGGCAACATGTTGCAAGAATTAGAAACGGTTTGATTGAAAATAAAGAATATGAAATTGTTGGAAGCTCGGTGGTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCACGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGTATGATTCTTTCCTAATTACTTTTTTACTATGCTTTGTTGCATTCTTCAACAGTTGAACTTATGTGCATGTTTTTCTCTTAATTTTCAGGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATATTGCCAGCCATTTACAGGTTCATTTTTGTATTAATTTTTTGTTGATTCTGTAAATTTGATATATTTTACCTAAGTCTATAGTTTCTCAGTTATCTTTTACCTAAGTCTGTTGTTGTAAATCATTTATGTTGTTTATACCTTAGTCTTGCATGTTATTCAAACTATTGAAGTTGTAAAGTAGTGAATTATGCAGTCATGGCAGATCTTGAAGTCTAAGTTTTAGAATAGAATATAAGTATTTCTCCATTAGCTTCTATACATCCTCTTGTTGATAATATGCTACTTGTCTTTTGTGTGATTTTAAACTGAAATTTATGGAGTATTATTATTAACATTGTAACTGCAATGTTGTTTTGCAGAAATATAGAAATCGGTTAAAGAGCTCAACAGAAGAGACAAAAGGTAGGATGAAAAAATCAAAGACGAGTCCTCTTGAAACAAAATTTGACTTAGAAGCTACTCACTCAATGCCGGAGCAGGACCAGAGTTTTCTCCTGAATTCTGCGACACAATGTCGCGACAATTGCGATGCACAACGACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>MS.gene78804.t1

MAVFTDNPSFPAGLKVIAIDHDITLLNSIEEMCNRYHYQVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLNIPVIMMAVDSTKSSIMKSIECGACEYWTKPLVEKEVKIMWQHVARIRNGLIENKEYEIVGSSVVQENRKRGREDANASKETLAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHIASHLQKYRNRLKSSTEETKGRMKKSKTSPLETKFDLEATHSMPEQDQSFLLNSATQCRDNCDAQRHWVEIGESNIMSNTFSDLPNLFPYVDDDLSSLVW