AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78807

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78807.t1 MTR_4g021855 84.718 301 45 1 1 300 1 301 0.0 526
MS.gene78807.t1 MTR_4g021790 78.000 300 40 1 1 300 1 274 5.04e-171 474
MS.gene78807.t1 MTR_4g021845 83.410 217 36 0 1 217 1 217 3.25e-134 379
MS.gene78807.t1 MTR_4g021760 71.756 262 73 1 1 261 1 262 7.95e-132 377
MS.gene78807.t1 MTR_5g055260 61.364 264 75 4 1 264 1 237 3.36e-98 289
MS.gene78807.t1 MTR_4g023980 75.373 134 33 0 58 191 38 171 3.11e-72 220
MS.gene78807.t1 MTR_1g032570 46.000 250 98 4 10 225 11 257 3.98e-67 221
MS.gene78807.t1 MTR_6g045327 45.833 240 119 5 10 247 14 244 6.50e-58 191
MS.gene78807.t1 MTR_2g034960 40.698 258 126 3 10 243 29 283 6.42e-56 191
MS.gene78807.t1 MTR_4g121020 42.213 244 111 3 10 225 31 272 3.34e-55 190
MS.gene78807.t1 MTR_4g121020 42.213 244 111 3 10 225 31 272 3.99e-55 189
MS.gene78807.t1 MTR_3g086100 42.213 244 113 5 10 225 13 256 1.97e-54 186
MS.gene78807.t1 MTR_8g105600 42.213 244 121 2 10 233 9 252 9.64e-54 184
MS.gene78807.t1 MTR_8g079940 41.152 243 117 4 9 225 24 266 1.72e-53 182
MS.gene78807.t1 MTR_4g131600 39.496 238 121 4 10 224 20 257 7.08e-48 169
MS.gene78807.t1 MTR_1g013170 38.866 247 123 3 7 225 2 248 1.53e-46 163
MS.gene78807.t1 MTR_3g106220 38.247 251 117 4 10 225 8 255 1.72e-46 166
MS.gene78807.t1 MTR_7g117705 35.366 246 131 3 7 224 2 247 1.21e-44 157
MS.gene78807.t1 MTR_1g013180 36.800 250 130 3 7 228 153 402 1.24e-43 158
MS.gene78807.t1 MTR_1g032570 43.455 191 72 3 68 225 1 188 1.42e-43 156
MS.gene78807.t1 MTR_4g131570 35.600 250 133 4 10 231 17 266 7.51e-43 155
MS.gene78807.t1 MTR_4g131580 36.032 247 133 4 10 231 17 263 1.83e-42 154
MS.gene78807.t1 MTR_2g450070 34.252 254 130 4 10 226 18 271 9.04e-42 153
MS.gene78807.t1 MTR_2g450070 34.252 254 130 4 10 226 18 271 1.24e-41 152
MS.gene78807.t1 MTR_6g016850 44.172 163 88 2 10 169 9 171 1.27e-41 142
MS.gene78807.t1 MTR_8g093040 40.426 235 117 6 1 229 1 218 1.43e-41 145
MS.gene78807.t1 MTR_3g102600 36.406 217 102 2 10 225 8 189 6.51e-40 139
MS.gene78807.t1 MTR_3g086100 41.622 185 81 4 68 225 1 185 1.00e-36 137
MS.gene78807.t1 MTR_3g102590 35.981 214 110 4 10 221 8 196 2.47e-36 130
MS.gene78807.t1 MTR_3g102600 34.597 211 102 2 10 219 8 183 8.62e-36 127
MS.gene78807.t1 MTR_3g102590 35.238 210 109 4 10 217 8 192 1.65e-33 122
MS.gene78807.t1 MTR_7g026400 37.321 209 98 5 45 229 19 218 6.88e-33 127
MS.gene78807.t1 MTR_3g102600 36.207 174 76 1 52 225 2 140 1.50e-32 119
MS.gene78807.t1 MTR_0450s0040 31.330 233 145 3 12 233 6 234 1.91e-30 117
MS.gene78807.t1 MTR_4g121020 40.606 165 69 2 88 225 6 168 6.37e-29 116
MS.gene78807.t1 MTR_3g102600 33.929 168 76 1 52 219 2 134 2.72e-28 106
MS.gene78807.t1 MTR_5g041350 61.818 55 20 1 168 221 15 69 5.03e-17 80.5
MS.gene78807.t1 MTR_6g032990 63.636 55 19 1 168 221 42 96 1.69e-16 79.3
MS.gene78807.t1 MTR_8g032710 26.038 265 148 8 12 229 19 282 2.03e-16 79.7
MS.gene78807.t1 MTR_1g013160 31.081 148 75 2 71 191 1 148 6.23e-16 74.3
MS.gene78807.t1 MTR_2g086450 60.000 55 21 1 168 221 45 99 2.02e-15 76.3
MS.gene78807.t1 MTR_7g098250 48.052 77 39 1 161 236 255 331 8.86e-15 74.7
MS.gene78807.t1 MTR_7g115530 50.685 73 33 2 168 237 218 290 2.69e-14 72.4
MS.gene78807.t1 MTR_2g016220 48.649 74 32 2 153 220 69 142 3.36e-14 72.8
MS.gene78807.t1 MTR_2g016220 48.649 74 32 2 153 220 69 142 3.57e-14 72.4
MS.gene78807.t1 MTR_7g089010 55.932 59 26 0 167 225 90 148 3.59e-14 72.0
MS.gene78807.t1 MTR_7g115530 50.685 73 33 2 168 237 218 290 4.29e-14 72.0
MS.gene78807.t1 MTR_4g064730 54.237 59 27 0 167 225 130 188 4.60e-14 71.6
MS.gene78807.t1 MTR_4g081710 56.364 55 23 1 168 221 17 71 5.86e-14 71.6
MS.gene78807.t1 MTR_7g115530 50.685 73 33 2 168 237 218 290 6.39e-14 72.0
MS.gene78807.t1 MTR_2g090675 45.556 90 46 3 137 224 129 217 7.17e-14 72.0
MS.gene78807.t1 MTR_1g080330 48.684 76 36 2 164 238 171 244 9.50e-14 70.5
MS.gene78807.t1 MTR_1g080330 44.828 87 45 2 164 249 171 255 1.25e-13 70.9
MS.gene78807.t1 MTR_7g115530 53.731 67 28 2 168 233 218 282 1.30e-13 70.9
MS.gene78807.t1 MTR_7g088070 53.125 64 27 2 168 228 50 113 2.52e-13 69.3
MS.gene78807.t1 MTR_1g093080 47.500 80 34 2 149 227 199 271 2.76e-13 70.1
MS.gene78807.t1 MTR_4g078810 55.172 58 26 0 167 224 157 214 3.16e-13 70.1
MS.gene78807.t1 MTR_7g088070 56.364 55 23 1 168 221 50 104 4.20e-13 68.6
MS.gene78807.t1 MTR_2g016220 42.857 84 41 3 153 229 69 152 5.26e-13 68.9
MS.gene78807.t1 MTR_5g014040 48.718 78 39 1 167 244 310 386 5.83e-13 69.3
MS.gene78807.t1 MTR_4g098870 50.725 69 33 1 167 235 329 396 6.15e-13 69.3
MS.gene78807.t1 MTR_4g098870 50.725 69 33 1 167 235 309 376 6.15e-13 69.3
MS.gene78807.t1 MTR_4g098870 55.357 56 25 0 167 222 309 364 9.96e-13 68.6
MS.gene78807.t1 MTR_4g098870 55.357 56 25 0 167 222 329 384 9.96e-13 68.6
MS.gene78807.t1 MTR_4g098870 50.725 69 33 1 167 235 348 415 1.03e-12 68.6
MS.gene78807.t1 MTR_0223s0040 53.571 56 25 1 166 220 16 71 1.07e-12 67.4
MS.gene78807.t1 MTR_4g086835 51.667 60 29 0 166 225 272 331 1.22e-12 68.2
MS.gene78807.t1 MTR_7g069660 51.786 56 26 1 166 220 69 124 1.30e-12 67.8
MS.gene78807.t1 MTR_2g027860 53.425 73 30 3 168 236 49 121 1.64e-12 67.4
MS.gene78807.t1 MTR_1g112370 38.710 93 44 4 166 255 20 102 2.24e-12 67.0
MS.gene78807.t1 MTR_7g093030 53.571 56 25 1 166 220 210 265 2.60e-12 66.6
MS.gene78807.t1 MTR_1g053835 52.632 57 26 1 166 221 256 312 2.64e-12 67.0
MS.gene78807.t1 MTR_3g450310 53.571 56 25 1 166 220 17 72 2.76e-12 65.1
MS.gene78807.t1 MTR_4g113140 46.753 77 39 2 158 233 226 301 3.22e-12 67.0
MS.gene78807.t1 MTR_7g093030 53.571 56 25 1 166 220 210 265 3.38e-12 66.6
MS.gene78807.t1 MTR_7g115530 59.615 52 20 1 168 218 218 269 7.90e-12 64.7
MS.gene78807.t1 MTR_2g023580 53.571 56 25 1 166 220 53 108 9.70e-12 63.9
MS.gene78807.t1 MTR_8g086410 50.000 56 27 1 166 220 107 162 1.24e-11 65.1
MS.gene78807.t1 MTR_8g086410 50.000 56 27 1 166 220 109 164 1.25e-11 65.1
MS.gene78807.t1 MTR_6g444980 56.364 55 23 1 168 221 35 89 1.48e-11 64.7
MS.gene78807.t1 MTR_6g444980 54.237 59 26 1 164 221 31 89 1.67e-11 64.3
MS.gene78807.t1 MTR_5g017980 51.667 60 29 0 166 225 190 249 1.68e-11 64.3
MS.gene78807.t1 MTR_5g017980 51.667 60 29 0 166 225 191 250 1.79e-11 64.3
MS.gene78807.t1 MTR_1g053800 53.968 63 28 1 162 223 171 233 1.85e-11 64.3
MS.gene78807.t1 MTR_5g054300 33.333 114 71 2 114 226 148 257 1.97e-11 64.3
MS.gene78807.t1 MTR_1g090670 56.364 55 23 1 168 221 44 98 2.65e-11 63.5
MS.gene78807.t1 MTR_5g027440 46.377 69 34 2 164 229 21 89 3.95e-11 62.8
MS.gene78807.t1 MTR_1g053830 49.057 53 26 1 170 221 20 72 4.03e-11 59.3
MS.gene78807.t1 MTR_4g086835 50.820 61 29 1 166 225 272 332 4.39e-11 63.5
MS.gene78807.t1 MTR_2g084230 43.243 74 38 2 148 220 80 150 5.37e-11 62.8
MS.gene78807.t1 MTR_2g084230 50.000 56 27 1 166 220 95 150 5.57e-11 63.2
MS.gene78807.t1 MTR_5g014040 45.783 83 39 2 167 244 310 391 6.09e-11 63.2
MS.gene78807.t1 MTR_5g014040 45.783 83 39 2 167 244 270 351 6.46e-11 63.2
MS.gene78807.t1 MTR_2g027800 51.667 60 28 1 168 226 39 98 7.03e-11 62.4
MS.gene78807.t1 MTR_2g027800 50.000 62 30 1 168 228 39 100 9.84e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78807.t1 AT2G01760 39.544 263 120 4 7 232 4 264 1.78e-56 187
MS.gene78807.t1 AT3G16857 37.086 302 137 7 10 261 33 331 1.33e-51 180
MS.gene78807.t1 AT3G16857 37.086 302 137 7 10 261 33 331 1.62e-51 180
MS.gene78807.t1 AT2G01760 36.082 291 119 6 7 232 4 292 1.30e-50 172
MS.gene78807.t1 AT1G67710 40.164 244 117 4 11 225 8 251 3.30e-48 168
MS.gene78807.t1 AT4G31920 38.983 236 126 5 10 230 13 245 1.70e-45 162
MS.gene78807.t1 AT2G25180 38.843 242 120 6 10 225 13 252 4.65e-43 155
MS.gene78807.t1 AT3G16857 35.741 263 116 7 49 261 2 261 2.40e-39 145
MS.gene78807.t1 AT5G58080 37.165 261 128 8 10 243 14 265 5.12e-38 142
MS.gene78807.t1 AT2G01760 37.313 201 88 3 68 232 1 199 2.03e-36 132
MS.gene78807.t1 AT3G62670 32.735 223 127 6 27 226 52 274 1.88e-32 124
MS.gene78807.t1 AT1G49190 28.839 267 153 7 10 243 30 292 3.57e-31 120
MS.gene78807.t1 AT1G67710 39.785 186 84 3 68 225 1 186 4.20e-31 121
MS.gene78807.t1 AT5G49240 32.627 236 132 5 9 217 37 272 1.41e-30 117
MS.gene78807.t1 AT5G07210 31.852 270 128 8 14 229 16 283 2.28e-28 115
MS.gene78807.t1 AT5G07210 30.935 278 128 9 14 229 16 291 7.37e-26 107
MS.gene78807.t1 AT1G67710 38.554 166 74 3 88 225 12 177 8.65e-23 98.2
MS.gene78807.t1 AT2G27070 29.054 296 147 11 14 252 16 305 2.01e-21 94.7
MS.gene78807.t1 AT1G68210 24.825 286 158 6 8 237 7 291 2.33e-19 88.6
MS.gene78807.t1 AT1G49190 33.871 124 77 2 10 130 30 151 4.82e-18 84.7
MS.gene78807.t1 AT3G04030 61.818 55 20 1 168 221 45 99 3.73e-16 78.6
MS.gene78807.t1 AT3G04030 61.818 55 20 1 168 221 45 99 4.06e-16 78.6
MS.gene78807.t1 AT5G18240 61.818 55 20 1 168 221 45 99 8.91e-16 77.4
MS.gene78807.t1 AT5G18240 61.818 55 20 1 168 221 45 99 8.91e-16 77.4
MS.gene78807.t1 AT5G18240 61.818 55 20 1 168 221 45 99 8.99e-16 77.4
MS.gene78807.t1 AT5G18240 61.818 55 20 1 168 221 45 99 9.24e-16 77.4
MS.gene78807.t1 AT5G18240 61.818 55 20 1 168 221 45 99 9.24e-16 77.4
MS.gene78807.t1 AT5G29000 38.938 113 61 3 134 245 204 309 1.47e-15 77.0
MS.gene78807.t1 AT5G29000 38.938 113 61 3 134 245 161 266 1.51e-15 76.6
MS.gene78807.t1 AT5G29000 38.938 113 61 3 134 245 161 266 1.51e-15 76.6
MS.gene78807.t1 AT2G01060 49.398 83 39 2 157 236 4 86 1.99e-15 75.5
MS.gene78807.t1 AT5G29000 45.455 88 40 3 134 220 204 284 3.68e-15 75.1
MS.gene78807.t1 AT3G04030 57.407 54 23 0 168 221 45 98 4.54e-15 75.5
MS.gene78807.t1 AT3G46640 49.296 71 36 0 167 237 143 213 1.06e-14 73.6
MS.gene78807.t1 AT3G10760 50.000 72 34 1 154 225 93 162 1.07e-14 73.9
MS.gene78807.t1 AT5G05090 51.471 68 31 1 158 225 73 138 1.07e-14 73.2
MS.gene78807.t1 AT5G59570 53.333 60 28 0 166 225 139 198 1.12e-14 73.6
MS.gene78807.t1 AT5G59570 53.333 60 28 0 166 225 139 198 1.12e-14 73.6
MS.gene78807.t1 AT3G46640 49.296 71 36 0 167 237 143 213 1.21e-14 73.6
MS.gene78807.t1 AT3G46640 49.296 71 36 0 167 237 143 213 1.21e-14 73.6
MS.gene78807.t1 AT3G04450 50.794 63 30 1 159 220 229 291 2.01e-14 73.6
MS.gene78807.t1 AT3G04450 50.794 63 30 1 159 220 189 251 2.15e-14 73.6
MS.gene78807.t1 AT3G04450 50.794 63 30 1 159 220 189 251 2.15e-14 73.6
MS.gene78807.t1 AT3G04450 50.794 63 30 1 159 220 229 291 2.28e-14 72.8
MS.gene78807.t1 AT2G40970 54.237 59 27 0 167 225 104 162 3.55e-14 71.2
MS.gene78807.t1 AT5G44190 58.621 58 24 0 167 224 146 203 7.71e-14 71.6
MS.gene78807.t1 AT5G44190 58.621 58 24 0 167 224 146 203 9.31e-14 71.2
MS.gene78807.t1 AT4G28610 60.000 55 21 1 168 221 225 279 1.53e-13 70.9
MS.gene78807.t1 AT1G69580 49.231 65 32 1 168 231 34 98 4.86e-13 68.9
MS.gene78807.t1 AT1G69580 49.231 65 32 1 168 231 34 98 4.89e-13 68.9
MS.gene78807.t1 AT2G20570 50.000 74 33 1 167 236 152 225 5.26e-13 69.3
MS.gene78807.t1 AT1G14600 61.538 52 19 1 170 220 25 76 1.40e-12 66.6
MS.gene78807.t1 AT1G25550 48.000 75 38 1 154 227 195 269 1.47e-12 67.8
MS.gene78807.t1 AT3G24120 38.462 104 58 3 168 270 41 139 2.03e-12 67.0
MS.gene78807.t1 AT3G24120 38.462 104 58 3 168 270 41 139 2.07e-12 67.0
MS.gene78807.t1 AT2G40260 49.275 69 31 2 166 233 80 145 2.40e-12 67.4
MS.gene78807.t1 AT3G13040 50.769 65 30 2 159 221 231 295 2.56e-12 67.4
MS.gene78807.t1 AT3G13040 50.769 65 30 2 159 221 231 295 2.56e-12 67.4
MS.gene78807.t1 AT3G13040 50.769 65 30 2 159 221 231 295 2.56e-12 67.4
MS.gene78807.t1 AT5G06800 58.182 55 22 1 168 221 192 246 3.25e-12 66.2
MS.gene78807.t1 AT2G20570 50.704 71 31 1 170 236 171 241 3.30e-12 67.0
MS.gene78807.t1 AT5G45580 37.838 111 60 3 164 272 3 106 3.82e-12 65.9
MS.gene78807.t1 AT4G13640 51.471 68 29 2 168 231 37 104 3.93e-12 65.9
MS.gene78807.t1 AT4G13640 51.471 68 29 2 168 231 37 104 4.18e-12 65.9
MS.gene78807.t1 AT5G45580 37.838 111 60 3 164 272 19 122 4.36e-12 65.9
MS.gene78807.t1 AT5G06800 50.000 68 31 2 168 234 192 257 4.84e-12 66.2
MS.gene78807.t1 AT5G06800 58.182 55 22 1 168 221 192 246 5.10e-12 66.2
MS.gene78807.t1 AT4G04605 50.000 66 31 2 166 229 12 77 5.73e-12 64.3
MS.gene78807.t1 AT1G79430 48.000 75 34 2 168 237 34 108 7.84e-12 65.5
MS.gene78807.t1 AT2G20400 59.259 54 21 1 168 220 231 284 9.70e-12 65.5
MS.gene78807.t1 AT2G20400 59.259 54 21 1 168 220 231 284 9.72e-12 65.5
MS.gene78807.t1 AT3G12730 52.727 55 25 1 168 221 23 77 1.73e-11 63.5
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.67e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.67e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.67e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.67e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.67e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene78807.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene78807.t1 AT2G03500 50.000 62 30 1 167 227 232 293 2.78e-11 64.3
MS.gene78807.t1 AT2G38300 48.214 56 28 1 166 220 52 107 5.70e-11 62.8
MS.gene78807.t1 AT2G42660 50.943 53 25 1 169 220 51 103 9.46e-11 61.6

Find 70 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATGATTCTCAAAATTA+TGG 0.243357 4.2:+80725091 MS.gene78807:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 4.2:+80725136 MS.gene78807:CDS
AACTGCATCAACGATTTCTA+TGG 0.292867 4.2:+80724944 MS.gene78807:CDS
ATTATGGATGTTCCTGGTTT+AGG 0.300681 4.2:+80725107 MS.gene78807:CDS
ATCCTACCTTTACAACCTTA+TGG 0.307805 4.2:+80725503 MS.gene78807:CDS
CTCAAAATTATGGATGTTCC+TGG 0.317981 4.2:+80725101 MS.gene78807:CDS
CTTGAATTTGTTGGAAAGAA+TGG 0.358508 4.2:+80724476 MS.gene78807:CDS
CAGTTTGAATGTTATCGTTC+CGG 0.359832 4.2:-80725419 None:intergenic
AGAAATCGTTGATGCAGTTC+TGG 0.372917 4.2:-80724940 None:intergenic
TTCTGATGCCTTGAATTTGT+TGG 0.373586 4.2:+80724467 MS.gene78807:CDS
AAAGTGACTAACCTTGATAA+TGG 0.378712 4.2:-80724309 None:intergenic
TACATATTGGACTAAGCCTT+TGG 0.386876 4.2:+80724744 MS.gene78807:CDS
TAGCTGAGTGGATTGATTGC+GGG 0.403037 4.2:+80725475 MS.gene78807:CDS
GGTTGAAGAAAATAGAAAAC+GGG 0.411991 4.2:+80724855 MS.gene78807:CDS
ATCAGAAGCTGTGTTACATT+TGG 0.412873 4.2:-80724451 None:intergenic
AATGTGGGGCTTGTACATAT+TGG 0.414028 4.2:+80724731 MS.gene78807:CDS
AATAAAGAATATGAAATTGT+TGG 0.416766 4.2:+80724823 MS.gene78807:CDS
TCAAATTCAATCGATCAATC+AGG 0.418663 4.2:+80725395 MS.gene78807:CDS
CATTCTTTCCAACAAATTCA+AGG 0.419108 4.2:-80724475 None:intergenic
AAACTGCATATTAGGCCATA+AGG 0.423301 4.2:-80725518 None:intergenic
AATGTCGTTTGCAGAAATAT+AGG 0.426550 4.2:+80725265 MS.gene78807:intron
GAGTGGATTGATTGCGGGAC+GGG 0.433574 4.2:+80725480 MS.gene78807:CDS
CTTTGCATGTGTATCTTTCG+AGG 0.437721 4.2:-80724896 None:intergenic
TGTAATCGATTCCATTATCA+AGG 0.450836 4.2:+80724298 MS.gene78807:CDS
TTTGCATGTGTATCTTTCGA+GGG 0.456538 4.2:-80724895 None:intergenic
TTCTGGCGACCAAGACAAAC+GGG 0.460294 4.2:-80724923 None:intergenic
AATAAATTCTTACTAATGAC+AGG 0.461511 4.2:-80724588 None:intergenic
ATATGAAATTGTTGGAAGCT+TGG 0.461542 4.2:+80724831 MS.gene78807:CDS
TTGAGACCAGCAGGGAATGA+TGG 0.467536 4.2:-80724223 None:intergenic
AAGTCATAAGCATCCATGTT+TGG 0.471983 4.2:-80724534 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4.2:+80724914 MS.gene78807:CDS
GAAAGATACACATGCAAAGA+AGG 0.490287 4.2:+80724900 MS.gene78807:CDS
CACTTTCCTCAACTCTATTG+AGG 0.492388 4.2:+80724270 MS.gene78807:CDS
CAGCTAGAAAACCATCATTC+CGG 0.492887 4.2:-80725458 None:intergenic
GTTCTGGCGACCAAGACAAA+CGG 0.493157 4.2:-80724924 None:intergenic
GCAATAACCTTGAGACCAGC+AGG 0.494358 4.2:-80724232 None:intergenic
CTAGCTGAGTGGATTGATTG+CGG 0.505708 4.2:+80725474 MS.gene78807:CDS
TGGTTGAAGAAAATAGAAAA+CGG 0.506154 4.2:+80724854 MS.gene78807:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4.2:-80724984 None:intergenic
GATGTTCCTGGTTTAGGTGT+AGG 0.515547 4.2:+80725113 MS.gene78807:CDS
ACTGATCACGACCGGAATGA+TGG 0.518029 4.2:+80725447 MS.gene78807:CDS
CTATGGGCTGTCAATCAACT+AGG 0.518108 4.2:+80724961 MS.gene78807:CDS
ATCATTCCCTGCTGGTCTCA+AGG 0.520371 4.2:+80724225 MS.gene78807:CDS
TTATGAAGTCTATTCAATGT+GGG 0.525603 4.2:+80724716 MS.gene78807:CDS
ATTAGGCCATAAGGTTGTAA+AGG 0.531488 4.2:-80725509 None:intergenic
TTGACGTCTTCTTCAACCAA+AGG 0.537631 4.2:-80724760 None:intergenic
GACAACCCATCATTCCCTGC+TGG 0.539365 4.2:+80724217 MS.gene78807:CDS
TGAGACCAGCAGGGAATGAT+GGG 0.539790 4.2:-80724222 None:intergenic
TTCAATCGATCAATCAGGAC+CGG 0.542488 4.2:+80725400 MS.gene78807:CDS
TGGCAACATGTTGTCAGAAA+CGG 0.543020 4.2:+80724790 MS.gene78807:CDS
ATTATGAAGTCTATTCAATG+TGG 0.558796 4.2:+80724715 MS.gene78807:CDS
ATTGACAAGGTTCATAGTGA+TGG 0.561362 4.2:+80725369 MS.gene78807:CDS
CTGAGCTGACTGATCACGAC+CGG 0.562799 4.2:+80725439 MS.gene78807:CDS
TGAAATTGTTGGAAGCTTGG+TGG 0.576315 4.2:+80724834 MS.gene78807:CDS
GAAAGTGATGTCATGATCAA+TGG 0.586165 4.2:-80724254 None:intergenic
ACTGCATCAACGATTTCTAT+GGG 0.587946 4.2:+80724945 MS.gene78807:CDS
TCAAGACGAGTGCATTGACA+AGG 0.590781 4.2:+80725356 MS.gene78807:CDS
GGCCATAAGGTTGTAAAGGT+AGG 0.602949 4.2:-80725505 None:intergenic
TGATGCTCATATGCCAAACA+TGG 0.604750 4.2:+80724521 MS.gene78807:CDS
TATGAAGTCTATTCAATGTG+GGG 0.614025 4.2:+80724717 MS.gene78807:CDS
ACATCTCCTCAATAGAGTTG+AGG 0.625514 4.2:-80724276 None:intergenic
TTGCATGTGTATCTTTCGAG+GGG 0.630230 4.2:-80724894 None:intergenic
TGAGTGGATTGATTGCGGGA+CGG 0.641804 4.2:+80725479 MS.gene78807:CDS
AAGAAGACGTCAAGAGCATG+TGG 0.649026 4.2:+80724770 MS.gene78807:CDS
CTGATGAAACAAAACGTGGG+AGG 0.661820 4.2:+80725307 MS.gene78807:CDS
ACATGTCCTACACCTAAACC+AGG 0.664952 4.2:-80725119 None:intergenic
CAATAACCTTGAGACCAGCA+GGG 0.699486 4.2:-80724231 None:intergenic
CAGCTGATGAAACAAAACGT+GGG 0.702126 4.2:+80725304 MS.gene78807:CDS
TCAGCTGATGAAACAAAACG+TGG 0.708886 4.2:+80725303 MS.gene78807:CDS
GTTGAAGAAAATAGAAAACG+GGG 0.712341 4.2:+80724856 MS.gene78807:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAGTGAGATAAAAA+TGG - chr4.2:80725058-80725077 None:intergenic 15.0%
!! AATAAAGAATATGAAATTGT+TGG + chr4.2:80724823-80724842 MS.gene78807:CDS 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4.2:80725092-80725111 None:intergenic 15.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4.2:80724591-80724610 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4.2:80725091-80725110 MS.gene78807:CDS 20.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4.2:80725093-80725112 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG + chr4.2:80724669-80724688 MS.gene78807:intron 20.0%
! ACAAAGCAAAGAAAAGAATT+AGG - chr4.2:80725007-80725026 None:intergenic 25.0%
! ATTATGAAGTCTATTCAATG+TGG + chr4.2:80724715-80724734 MS.gene78807:CDS 25.0%
! TGGTTGAAGAAAATAGAAAA+CGG + chr4.2:80724854-80724873 MS.gene78807:CDS 25.0%
! TTATGAAGTCTATTCAATGT+GGG + chr4.2:80724716-80724735 MS.gene78807:CDS 25.0%
AAAGTGACTAACCTTGATAA+TGG - chr4.2:80724312-80724331 None:intergenic 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4.2:80724987-80725006 None:intergenic 30.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4.2:80724831-80724850 MS.gene78807:CDS 30.0%
CATTCTTTCCAACAAATTCA+AGG - chr4.2:80724478-80724497 None:intergenic 30.0%
GGTTGAAGAAAATAGAAAAC+GGG + chr4.2:80724855-80724874 MS.gene78807:CDS 30.0%
GTTGAAGAAAATAGAAAACG+GGG + chr4.2:80724856-80724875 MS.gene78807:CDS 30.0%
TATGAAGTCTATTCAATGTG+GGG + chr4.2:80724717-80724736 MS.gene78807:CDS 30.0%
TCAAATTCAATCGATCAATC+AGG + chr4.2:80725395-80725414 MS.gene78807:CDS 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4.2:80725265-80725284 MS.gene78807:intron 30.0%
! TGTAATCGATTCCATTATCA+AGG + chr4.2:80724298-80724317 MS.gene78807:CDS 30.0%
!! CTTGAATTTGTTGGAAAGAA+TGG + chr4.2:80724476-80724495 MS.gene78807:CDS 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG + chr4.2:80725147-80725166 MS.gene78807:intron 30.0%
!!! TGAGTTTTAGGGTAACATTT+TGG - chr4.2:80724563-80724582 None:intergenic 30.0%
AACTGCATCAACGATTTCTA+TGG + chr4.2:80724944-80724963 MS.gene78807:CDS 35.0%
AAGTCATAAGCATCCATGTT+TGG - chr4.2:80724537-80724556 None:intergenic 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4.2:80725136-80725155 MS.gene78807:CDS 35.0%
ACTGCATCAACGATTTCTAT+GGG + chr4.2:80724945-80724964 MS.gene78807:CDS 35.0%
ATCAGAAGCTGTGTTACATT+TGG - chr4.2:80724454-80724473 None:intergenic 35.0%
ATCCTACCTTTACAACCTTA+TGG + chr4.2:80725503-80725522 MS.gene78807:CDS 35.0%
ATTATGGATGTTCCTGGTTT+AGG + chr4.2:80725107-80725126 MS.gene78807:CDS 35.0%
ATTGACAAGGTTCATAGTGA+TGG + chr4.2:80725369-80725388 MS.gene78807:CDS 35.0%
CAGTTTGAATGTTATCGTTC+CGG - chr4.2:80725422-80725441 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+TGG + chr4.2:80725101-80725120 MS.gene78807:CDS 35.0%
GAAAGATACACATGCAAAGA+AGG + chr4.2:80724900-80724919 MS.gene78807:CDS 35.0%
GAAAGTGATGTCATGATCAA+TGG - chr4.2:80724257-80724276 None:intergenic 35.0%
TACATATTGGACTAAGCCTT+TGG + chr4.2:80724744-80724763 MS.gene78807:CDS 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4.2:80724467-80724486 MS.gene78807:CDS 35.0%
TTTGCATGTGTATCTTTCGA+GGG - chr4.2:80724898-80724917 None:intergenic 35.0%
! GACAGGAATGTTGAGTTTTA+GGG - chr4.2:80724574-80724593 None:intergenic 35.0%
! TGACAGGAATGTTGAGTTTT+AGG - chr4.2:80724575-80724594 None:intergenic 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG + chr4.2:80725151-80725170 MS.gene78807:intron 35.0%
AATGTGGGGCTTGTACATAT+TGG + chr4.2:80724731-80724750 MS.gene78807:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4.2:80724279-80724298 None:intergenic 40.0%
AGAAATCGTTGATGCAGTTC+TGG - chr4.2:80724943-80724962 None:intergenic 40.0%
CACTTTCCTCAACTCTATTG+AGG + chr4.2:80724270-80724289 MS.gene78807:CDS 40.0%
CAGCTAGAAAACCATCATTC+CGG - chr4.2:80725461-80725480 None:intergenic 40.0%
CAGCTGATGAAACAAAACGT+GGG + chr4.2:80725304-80725323 MS.gene78807:CDS 40.0%
CTTTGCATGTGTATCTTTCG+AGG - chr4.2:80724899-80724918 None:intergenic 40.0%
GTGGGAGGAAAAAATCAAAG+TGG + chr4.2:80725322-80725341 MS.gene78807:CDS 40.0%
TCAGCTGATGAAACAAAACG+TGG + chr4.2:80725303-80725322 MS.gene78807:CDS 40.0%
TGAAATTGTTGGAAGCTTGG+TGG + chr4.2:80724834-80724853 MS.gene78807:CDS 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4.2:80724521-80724540 MS.gene78807:CDS 40.0%
TGGCAACATGTTGTCAGAAA+CGG + chr4.2:80724790-80724809 MS.gene78807:CDS 40.0%
TTCAATCGATCAATCAGGAC+CGG + chr4.2:80725400-80725419 MS.gene78807:CDS 40.0%
TTGACGTCTTCTTCAACCAA+AGG - chr4.2:80724763-80724782 None:intergenic 40.0%
TTGCATGTGTATCTTTCGAG+GGG - chr4.2:80724897-80724916 None:intergenic 40.0%
!!! ATGATGGTTTTCTAGCTGAG+TGG + chr4.2:80725463-80725482 MS.gene78807:CDS 40.0%
ACATGTCCTACACCTAAACC+AGG - chr4.2:80725122-80725141 None:intergenic 45.0%
CAATAACCTTGAGACCAGCA+GGG - chr4.2:80724234-80724253 None:intergenic 45.0%
CTGATGAAACAAAACGTGGG+AGG + chr4.2:80725307-80725326 MS.gene78807:CDS 45.0%
GATGTTCCTGGTTTAGGTGT+AGG + chr4.2:80725113-80725132 MS.gene78807:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG - chr4.2:80725508-80725527 None:intergenic 45.0%
! AAGAAGACGTCAAGAGCATG+TGG + chr4.2:80724770-80724789 MS.gene78807:CDS 45.0%
! CTATGGGCTGTCAATCAACT+AGG + chr4.2:80724961-80724980 MS.gene78807:CDS 45.0%
! TCAAGACGAGTGCATTGACA+AGG + chr4.2:80725356-80725375 MS.gene78807:CDS 45.0%
!! CTAGCTGAGTGGATTGATTG+CGG + chr4.2:80725474-80725493 MS.gene78807:CDS 45.0%
!! TAGCTGAGTGGATTGATTGC+GGG + chr4.2:80725475-80725494 MS.gene78807:CDS 45.0%
ACTGATCACGACCGGAATGA+TGG + chr4.2:80725447-80725466 MS.gene78807:CDS 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4.2:80724914-80724933 MS.gene78807:CDS 50.0%
GCAATAACCTTGAGACCAGC+AGG - chr4.2:80724235-80724254 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4.2:80724927-80724946 None:intergenic 50.0%
TGAGACCAGCAGGGAATGAT+GGG - chr4.2:80724225-80724244 None:intergenic 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4.2:80724926-80724945 None:intergenic 50.0%
TTGAGACCAGCAGGGAATGA+TGG - chr4.2:80724226-80724245 None:intergenic 50.0%
! ATCATTCCCTGCTGGTCTCA+AGG + chr4.2:80724225-80724244 MS.gene78807:CDS 50.0%
!! TGAGTGGATTGATTGCGGGA+CGG + chr4.2:80725479-80725498 MS.gene78807:CDS 50.0%
CTGAGCTGACTGATCACGAC+CGG + chr4.2:80725439-80725458 MS.gene78807:CDS 55.0%
GACAACCCATCATTCCCTGC+TGG + chr4.2:80724217-80724236 MS.gene78807:CDS 55.0%
!! GAGTGGATTGATTGCGGGAC+GGG + chr4.2:80725480-80725499 MS.gene78807:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 80724202 80725530 80724202 ID=MS.gene78807
chr4.2 mRNA 80724202 80725530 80724202 ID=MS.gene78807.t1;Parent=MS.gene78807
chr4.2 exon 80724202 80724319 80724202 ID=MS.gene78807.t1.exon1;Parent=MS.gene78807.t1
chr4.2 CDS 80724202 80724319 80724202 ID=cds.MS.gene78807.t1;Parent=MS.gene78807.t1
chr4.2 exon 80724448 80724597 80724448 ID=MS.gene78807.t1.exon2;Parent=MS.gene78807.t1
chr4.2 CDS 80724448 80724597 80724448 ID=cds.MS.gene78807.t1;Parent=MS.gene78807.t1
chr4.2 exon 80724686 80724991 80724686 ID=MS.gene78807.t1.exon3;Parent=MS.gene78807.t1
chr4.2 CDS 80724686 80724991 80724686 ID=cds.MS.gene78807.t1;Parent=MS.gene78807.t1
chr4.2 exon 80725081 80725157 80725081 ID=MS.gene78807.t1.exon4;Parent=MS.gene78807.t1
chr4.2 CDS 80725081 80725157 80725081 ID=cds.MS.gene78807.t1;Parent=MS.gene78807.t1
chr4.2 exon 80725279 80725530 80725279 ID=MS.gene78807.t1.exon5;Parent=MS.gene78807.t1
chr4.2 CDS 80725279 80725530 80725279 ID=cds.MS.gene78807.t1;Parent=MS.gene78807.t1
Gene Sequence

>MS.gene78807

ATGGCTGTTTTCGTTGACAACCCATCATTCCCTGCTGGTCTCAAGGTTATTGCCATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATTCCATTATCAAGGTTAGTCACTTTGTTAATTTCACTTTATTCAATTCTGAATTTTCTATCTATGTTCTTACTTTTATGTATTTTCATGTTTGTTTGTTTCTTCATTCTTATGCATATTTTTATATGTTTCTTTATTTCAGTTACCAAATGTAACACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAATGGACTGTTTCGATTTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCTAAAACTCAACATTCCTGTCATTAGTAAGAATTTATTCAACTTTACATGATTCATTCATATATATGTACTTAATTAGAGTGAACTGACATTATTATTATTTCTTTTTTGTAGTGATGGCTGTTGATTTCGCAAAAAGTTCTATTATGAAGTCTATTCAATGTGGGGCTTGTACATATTGGACTAAGCCTTTGGTTGAAGAAGACGTCAAGAGCATGTGGCAACATGTTGTCAGAAACGGTTCGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTGGTTGAAGAAAATAGAAAACGGGGTAGAGAAGATGATAACCCCTCGAAAGATACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAACTGCATCAACGATTTCTATGGGCTGTCAATCAACTAGGCCTTGATAGTATGATTCTTTCCTAATTCTTTTCTTTGCTTTGTTTGTATTATTTAACAACTCAACTTATGTCCATTTTTATCTCACTTATTTTTCAGAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCTGGTTTAGGTGTAGGACATGTTGCTAGTCATTTACAGGTTTGTTTTCGGAAGGAGTCTAATTAAATTATTAATAATATACTACATGTATTTTGAGTGATTTTAAATTGAAACTTATAGAGTACTATTGTTAAAATTGTGATTGCAATGTCGTTTGCAGAAATATAGGATTCATTTGAAGAGATCAGCTGATGAAACAAAACGTGGGAGGAAAAAATCAAAGTGGAGTGATAGCTTTCAAGACGAGTGCATTGACAAGGTTCATAGTGATGGCTTTCAAATTCAATCGATCAATCAGGACCGGAACGATAACATTCAAACTGAGCTGACTGATCACGACCGGAATGATGGTTTTCTAGCTGAGTGGATTGATTGCGGGACGGGATCCTACCTTTACAACCTTATGGCCTAA
Protein sequence

>MS.gene78807.t1

MAVFVDNPSFPAGLKVIAIDHDITFLNSIEEMCNRFHYQVTKCNTASDALNLLERMDCFDLMLIDAHMPNMDAYDFVQNVTLKLNIPVIMMAVDFAKSSIMKSIQCGACTYWTKPLVEEDVKSMWQHVVRNGSTENKEYEIVGSLVVEENRKRGREDDNPSKDTHAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKRGRKKSKWSDSFQDECIDKVHSDGFQIQSINQDRNDNIQTELTDHDRNDGFLAEWIDCGTGSYLYNLMA