AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78827

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78827.t1 MTR_4g022370 97.527 364 8 1 1 363 1 364 0.0 724
MS.gene78827.t1 MTR_7g059400 50.000 384 125 15 1 359 1 342 3.45e-88 270
MS.gene78827.t1 MTR_4g063780 41.803 366 153 14 1 342 1 330 5.01e-67 215
MS.gene78827.t1 MTR_2g096740 40.719 334 112 18 1 314 1 268 8.00e-56 185
MS.gene78827.t1 MTR_2g096740 38.782 312 122 16 1 307 1 248 1.35e-48 166
MS.gene78827.t1 MTR_7g024670 36.829 391 183 17 1 353 1 365 6.80e-47 164
MS.gene78827.t1 MTR_3g091820 63.636 88 28 1 51 138 16 99 3.53e-32 123
MS.gene78827.t1 MTR_4g088580 52.459 122 52 2 83 200 56 175 2.48e-31 122
MS.gene78827.t1 MTR_2g093220 80.328 61 12 0 76 136 34 94 4.72e-31 119
MS.gene78827.t1 MTR_8g027295 45.324 139 65 3 69 205 11 140 6.59e-31 118
MS.gene78827.t1 MTR_8g479350 51.351 111 49 2 82 188 68 177 8.72e-30 117
MS.gene78827.t1 MTR_4g461080 65.385 78 26 1 57 134 5 81 3.65e-29 114
MS.gene78827.t1 MTR_5g031440 81.818 55 10 0 78 132 39 93 3.76e-29 115
MS.gene78827.t1 MTR_8g015840 75.000 68 15 2 74 140 13 79 5.23e-29 112
MS.gene78827.t1 MTR_3g077750 63.855 83 27 2 50 132 32 111 6.12e-29 115
MS.gene78827.t1 MTR_3g090430 64.865 74 26 0 63 136 32 105 6.51e-29 114
MS.gene78827.t1 MTR_8g079060 54.255 94 32 2 61 153 17 100 2.19e-28 110
MS.gene78827.t1 MTR_8g068210 69.841 63 19 0 79 141 29 91 2.25e-28 113
MS.gene78827.t1 MTR_2g014170 86.275 51 7 0 81 131 44 94 2.95e-28 112
MS.gene78827.t1 MTR_1g056810 56.250 96 36 1 84 179 14 103 2.81e-27 109
MS.gene78827.t1 MTR_2g059540 86.000 50 7 0 81 130 30 79 4.64e-27 106
MS.gene78827.t1 MTR_1g077600 55.000 80 34 1 59 136 18 97 9.31e-27 107
MS.gene78827.t1 MTR_1g077600 55.000 80 34 1 59 136 18 97 1.13e-26 107
MS.gene78827.t1 MTR_4g109980 80.769 52 10 0 81 132 39 90 1.32e-26 108
MS.gene78827.t1 MTR_2g014060 66.667 72 19 2 62 133 10 76 3.52e-26 107
MS.gene78827.t1 MTR_4g089095 74.138 58 14 1 82 139 50 106 6.83e-26 105
MS.gene78827.t1 MTR_4g089095 74.138 58 14 1 82 139 16 72 7.97e-26 104
MS.gene78827.t1 MTR_8g044220 68.333 60 19 0 78 137 114 173 8.11e-26 107
MS.gene78827.t1 MTR_6g012450 67.213 61 20 0 78 138 132 192 2.39e-25 107
MS.gene78827.t1 MTR_2g013370 71.930 57 15 1 80 136 44 99 2.55e-25 103
MS.gene78827.t1 MTR_2g016030 70.175 57 17 0 79 135 44 100 5.97e-25 99.8
MS.gene78827.t1 MTR_6g027450 62.903 62 23 0 75 136 82 143 7.31e-25 103
MS.gene78827.t1 MTR_7g010950 62.500 64 24 0 75 138 128 191 1.18e-24 105
MS.gene78827.t1 MTR_4g082060 65.079 63 22 0 78 140 118 180 1.31e-24 104
MS.gene78827.t1 MTR_5g041420 45.833 96 49 1 43 138 102 194 2.79e-24 102
MS.gene78827.t1 MTR_7g086780 62.295 61 23 0 75 135 92 152 3.82e-24 102
MS.gene78827.t1 MTR_3g435480 63.492 63 23 0 78 140 132 194 4.32e-24 103
MS.gene78827.t1 MTR_6g027460 60.656 61 24 0 75 135 89 149 6.65e-24 101
MS.gene78827.t1 MTR_5g041380 53.247 77 36 0 66 142 111 187 1.07e-22 98.2
MS.gene78827.t1 MTR_5g041400 48.235 85 44 0 58 142 95 179 4.45e-22 96.3
MS.gene78827.t1 MTR_5g041530 45.977 87 44 1 52 135 107 193 7.23e-22 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78827.t1 AT3G55370 38.243 387 168 17 1 350 1 353 4.59e-56 187
MS.gene78827.t1 AT3G55370 43.599 289 115 13 1 275 1 255 9.36e-56 185
MS.gene78827.t1 AT2G37590 41.337 329 148 11 1 308 1 305 5.16e-50 171
MS.gene78827.t1 AT3G55370 47.059 238 93 12 45 275 89 300 8.62e-49 168
MS.gene78827.t1 AT5G02460 39.096 376 141 13 1 312 1 352 6.85e-48 167
MS.gene78827.t1 AT2G28810 34.688 369 176 15 1 342 1 331 1.27e-38 141
MS.gene78827.t1 AT1G07640 50.694 144 65 3 5 142 2 145 5.47e-37 136
MS.gene78827.t1 AT5G60200 57.407 108 45 1 78 184 51 158 1.60e-36 133
MS.gene78827.t1 AT1G07640 53.731 134 56 3 15 142 4 137 1.80e-36 135
MS.gene78827.t1 AT2G28810 34.897 341 164 12 23 342 11 314 3.01e-36 134
MS.gene78827.t1 AT1G07640 70.886 79 23 0 64 142 3 81 3.59e-36 132
MS.gene78827.t1 AT3G45610 55.238 105 44 2 78 181 36 138 4.56e-34 126
MS.gene78827.t1 AT5G65590 38.559 236 101 9 42 246 8 230 3.77e-31 120
MS.gene78827.t1 AT1G51700 60.000 95 34 3 77 167 27 121 9.22e-31 116
MS.gene78827.t1 AT1G28310 79.310 58 12 0 82 139 27 84 3.32e-30 117
MS.gene78827.t1 AT2G28510 81.967 61 11 0 81 141 47 107 4.10e-30 117
MS.gene78827.t1 AT1G28310 79.310 58 12 0 82 139 51 108 5.17e-30 117
MS.gene78827.t1 AT5G60850 71.014 69 20 0 69 137 38 106 1.96e-29 115
MS.gene78827.t1 AT3G61850 75.410 61 15 0 78 138 58 118 6.02e-29 114
MS.gene78827.t1 AT3G61850 75.410 61 15 0 78 138 58 118 6.02e-29 114
MS.gene78827.t1 AT3G61850 75.410 61 15 0 78 138 70 130 8.16e-29 114
MS.gene78827.t1 AT3G21270 84.906 53 8 0 82 134 29 81 9.02e-29 111
MS.gene78827.t1 AT3G61850 75.410 61 15 0 78 138 58 118 9.94e-29 114
MS.gene78827.t1 AT3G61850 75.410 61 15 0 78 138 70 130 1.24e-28 114
MS.gene78827.t1 AT3G52440 58.140 86 36 0 79 164 22 107 4.19e-28 110
MS.gene78827.t1 AT4G38000 73.438 64 16 1 76 138 35 98 4.64e-28 110
MS.gene78827.t1 AT4G24060 73.770 61 16 0 76 136 47 107 6.84e-28 112
MS.gene78827.t1 AT3G52440 58.140 86 36 0 79 164 41 126 7.34e-28 110
MS.gene78827.t1 AT5G66940 48.958 96 38 2 63 147 2 97 4.78e-27 107
MS.gene78827.t1 AT1G21340 68.750 64 20 0 80 143 36 99 7.84e-27 107
MS.gene78827.t1 AT5G62940 86.000 50 7 0 83 132 74 123 1.78e-26 108
MS.gene78827.t1 AT2G34140 51.685 89 40 1 47 135 26 111 2.26e-26 103
MS.gene78827.t1 AT3G50410 69.492 59 18 0 82 140 30 88 3.00e-26 105
MS.gene78827.t1 AT4G00940 71.186 59 17 0 78 136 64 122 5.37e-26 106
MS.gene78827.t1 AT4G00940 71.186 59 17 0 78 136 64 122 5.37e-26 106
MS.gene78827.t1 AT4G00940 71.186 59 17 0 78 136 64 122 5.37e-26 106
MS.gene78827.t1 AT2G46590 78.846 52 11 0 78 129 64 115 2.08e-25 105
MS.gene78827.t1 AT2G46590 78.846 52 11 0 78 129 76 127 2.29e-25 105
MS.gene78827.t1 AT1G29160 68.966 58 18 0 78 135 58 115 5.38e-25 100
MS.gene78827.t1 AT4G21050 66.129 62 21 0 75 136 17 78 5.61e-25 101
MS.gene78827.t1 AT3G47500 64.062 64 23 0 75 138 103 166 1.08e-24 104
MS.gene78827.t1 AT1G64620 74.545 55 14 0 76 130 43 97 1.23e-24 103
MS.gene78827.t1 AT1G26790 55.072 69 31 0 67 135 96 164 3.53e-24 102
MS.gene78827.t1 AT1G69570 62.295 61 23 0 75 135 125 185 5.40e-24 102
MS.gene78827.t1 AT5G62430 58.209 67 28 0 75 141 47 113 1.31e-23 99.8
MS.gene78827.t1 AT5G39660 62.903 62 23 0 74 135 130 191 1.32e-23 102
MS.gene78827.t1 AT5G39660 62.903 62 23 0 74 135 130 191 1.32e-23 102
MS.gene78827.t1 AT5G39660 62.903 62 23 0 74 135 130 191 1.32e-23 102
MS.gene78827.t1 AT1G47655 68.333 60 17 1 73 130 18 77 1.93e-23 97.1
MS.gene78827.t1 AT4G21040 50.562 89 40 1 84 172 27 111 4.29e-22 94.0
MS.gene78827.t1 AT4G21080 47.674 86 41 1 84 169 27 108 8.12e-21 90.9
MS.gene78827.t1 AT4G21030 55.932 59 23 1 71 129 13 68 3.03e-14 71.2

Find 103 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAGGAAGTGAACAATTT+TGG 0.154411 4.2:-80503585 MS.gene78827:CDS
GATTTCGTCGGAGGAGATTT+AGG 0.156968 4.2:-80503952 MS.gene78827:CDS
AATTGGACTGATCATGTTTC+TGG 0.157222 4.2:-80503529 MS.gene78827:CDS
TCAACTTGCTTCTGTGAAAA+TGG 0.216358 4.2:-80503662 MS.gene78827:CDS
TGGAATAGTGCTGTTATTAC+TGG 0.251462 4.2:-80503565 MS.gene78827:CDS
CCAGAAGTTGAAATCACTTT+TGG 0.251474 4.2:+80503694 None:intergenic
GCAAGTCTTGCAGAAGTGTC+TGG 0.259253 4.2:+80504245 None:intergenic
CACCTCAAATGTCATCATTA+AGG 0.271108 4.2:-80504014 MS.gene78827:CDS
CCAAAAGTGATTTCAACTTC+TGG 0.271253 4.2:-80503694 MS.gene78827:CDS
GGTGATTTGGGAAGTGCTTT+AGG 0.279369 4.2:-80503895 MS.gene78827:CDS
GGAAGAGTGGATATTGTTAT+TGG 0.289267 4.2:+80504056 None:intergenic
ATGGGAGGAGTAGGTGATTT+GGG 0.291520 4.2:-80503907 MS.gene78827:CDS
CTGGTAATTCTGCAGCTAAT+TGG 0.303967 4.2:-80503546 MS.gene78827:CDS
GAGACGGAGCCGCCGTTTCC+TGG 0.321013 4.2:+80504480 None:intergenic
CATGGGAGGAGTAGGTGATT+TGG 0.324852 4.2:-80503908 MS.gene78827:CDS
TCTCCTCCGACGAAATCATT+AGG 0.340259 4.2:+80503958 None:intergenic
GGACAAGAGGCGGGGCTCTA+AGG 0.342022 4.2:-80504212 MS.gene78827:CDS
TCCCGGTCGGTGGAGGCTTT+CGG 0.354829 4.2:-80504185 MS.gene78827:CDS
ATTTCGTCGGAGGAGATTTA+GGG 0.356337 4.2:-80503951 MS.gene78827:CDS
AATTAGCCAGAAAAGGAAAT+TGG 0.359477 4.2:+80503800 None:intergenic
CACTCTTCCGCCGATATATT+AGG 0.387275 4.2:-80504042 MS.gene78827:CDS
GGAGATTTAGGGTTAAACTA+TGG 0.392754 4.2:-80503940 MS.gene78827:CDS
GCCCTATCGGACATCGATCC+TGG 0.393185 4.2:+80504372 None:intergenic
TGTGACTGTGGTGGTGGTGG+TGG 0.398669 4.2:+80504435 None:intergenic
GCAAGCTCGGACCGCCAAAC+GGG 0.407681 4.2:-80504108 MS.gene78827:CDS
AATGGAAGATAGTATCAATA+GGG 0.409067 4.2:-80503644 MS.gene78827:CDS
GTTTGGCGGTCCGAGCTTGC+CGG 0.412883 4.2:+80504111 None:intergenic
TTGAACTAGCACTCCCCGTT+TGG 0.413567 4.2:+80504094 None:intergenic
CTCCTCCGACGAAATCATTA+GGG 0.413580 4.2:+80503959 None:intergenic
AAATGGAAGATAGTATCAAT+AGG 0.414206 4.2:-80503645 MS.gene78827:CDS
CTTATCTTGATGCAGCCAAC+TGG 0.417162 4.2:-80504774 MS.gene78827:CDS
CATCAAGTGGTTTGGAGCAA+TGG 0.430190 4.2:-80503855 MS.gene78827:CDS
ACACTATAAAATTCTACATC+TGG 0.437923 4.2:+80503476 None:intergenic
GGCAAGCTCGGACCGCCAAA+CGG 0.446315 4.2:-80504109 MS.gene78827:CDS
GCAAGACTTGCAGAAGGTAC+TGG 0.454376 4.2:-80504233 MS.gene78827:CDS
TGCAGCCAACTGGCAGCAAC+AGG 0.457644 4.2:-80504764 MS.gene78827:intron
GAGGATCCACCGGCTCAATT+AGG 0.467697 4.2:-80504395 MS.gene78827:CDS
AACAAAGTCTCCGGCAAGCT+CGG 0.469322 4.2:-80504121 MS.gene78827:CDS
GTTCGCCATCCTTGCCCTAT+CGG 0.470894 4.2:+80504359 None:intergenic
CCCTCCAAATTAGCCAGAAA+AGG 0.471049 4.2:+80503793 None:intergenic
GAAGGTACTGGACAAGAGGC+GGG 0.472489 4.2:-80504221 MS.gene78827:CDS
TTTGAACATTTCCTTCAAAA+GGG 0.473490 4.2:+80503752 None:intergenic
AGGAATGTCCCGGTCGGTGG+AGG 0.474332 4.2:-80504192 MS.gene78827:CDS
CTCCGAAAGCCTCCACCGAC+CGG 0.477068 4.2:+80504183 None:intergenic
AACTAGCACTCCCCGTTTGG+CGG 0.478302 4.2:+80504097 None:intergenic
AGGTGTAAGGCCTAATATAT+CGG 0.483491 4.2:+80504032 None:intergenic
GGAAACAATAACAGTAATCA+AGG 0.492146 4.2:-80503601 MS.gene78827:CDS
ATGATGACATTTGAGGTGTA+AGG 0.497793 4.2:+80504019 None:intergenic
TTTAGTTACATGGGAGGAGT+AGG 0.502079 4.2:-80503916 MS.gene78827:CDS
TGAGATTGTGACTGTGGTGG+TGG 0.503091 4.2:+80504429 None:intergenic
CAAGTCTTGCAGAAGTGTCT+GGG 0.504581 4.2:+80504246 None:intergenic
GATTGTGACTGTGGTGGTGG+TGG 0.504773 4.2:+80504432 None:intergenic
AACCTTAATGATGACATTTG+AGG 0.519434 4.2:+80504012 None:intergenic
GGACATTTCAGTGCTGCCTC+CGG 0.520025 4.2:+80504327 None:intergenic
AGAGTGGATATTGTTATTGG+AGG 0.520652 4.2:+80504059 None:intergenic
TTCAGTGCTGCCTCCGGCAT+AGG 0.523439 4.2:+80504333 None:intergenic
CTAAGGAATGTCCCGGTCGG+TGG 0.528029 4.2:-80504195 MS.gene78827:CDS
TTACTGAAGTTATGATGTAA+AGG 0.534579 4.2:+80503982 None:intergenic
ATCAAGATAAGCTGCTGGTA+TGG 0.536728 4.2:+80504786 None:intergenic
ATTTACCTGTTGCTGCCAGT+TGG 0.539661 4.2:+80504759 None:intergenic
GATGGCGAACATGCCTATGC+CGG 0.544192 4.2:-80504346 MS.gene78827:CDS
CATCATCAGCAACCAGGAAA+CGG 0.545159 4.2:-80504492 MS.gene78827:CDS
CGATCCTGGCCTAATTGAGC+CGG 0.550851 4.2:+80504386 None:intergenic
TGGAAACTCAACAAAGTCTC+CGG 0.554123 4.2:-80504130 MS.gene78827:CDS
CCTCATGGAGGAGGATCCAC+CGG 0.558615 4.2:-80504405 MS.gene78827:CDS
TCCGAAAGCCTCCACCGACC+GGG 0.562265 4.2:+80504184 None:intergenic
GCCAGGATCGATGTCCGATA+GGG 0.563470 4.2:-80504373 MS.gene78827:CDS
CAGTCACAATCTCAACCTCA+TGG 0.566499 4.2:-80504420 MS.gene78827:CDS
TCAAGCAAAAGTAGCAACAA+TGG 0.567762 4.2:-80504150 MS.gene78827:CDS
TCCTGGCCTAATTGAGCCGG+TGG 0.569726 4.2:+80504389 None:intergenic
CAGAATCATCATCAGCAACC+AGG 0.572861 4.2:-80504498 MS.gene78827:CDS
GATGTCCGATAGGGCAAGGA+TGG 0.573727 4.2:-80504364 MS.gene78827:CDS
CAAGTGGTTTGGAGCAATGG+AGG 0.575412 4.2:-80503852 MS.gene78827:CDS
AGTAACCCTAATGATTTCGT+CGG 0.581465 4.2:-80503964 MS.gene78827:CDS
CTAATATATCGGCGGAAGAG+TGG 0.581651 4.2:+80504043 None:intergenic
TCCACCGGCTCAATTAGGCC+AGG 0.583672 4.2:-80504390 MS.gene78827:CDS
GATTTGGGAAGTGCTTTAGG+AGG 0.585086 4.2:-80503892 MS.gene78827:CDS
GGCCAGGATCGATGTCCGAT+AGG 0.588624 4.2:-80504374 MS.gene78827:CDS
CAATCTCAACCTCATGGAGG+AGG 0.588847 4.2:-80504414 MS.gene78827:CDS
CGGGGCTCTAAGGAATGTCC+CGG 0.591050 4.2:-80504202 MS.gene78827:CDS
GCTCTAAGGAATGTCCCGGT+CGG 0.592207 4.2:-80504198 MS.gene78827:CDS
CTGAAGCAGTTGTTGAGAGA+CGG 0.593094 4.2:+80504464 None:intergenic
GGCGAACATGCCTATGCCGG+AGG 0.593250 4.2:-80504343 MS.gene78827:CDS
AGAAGGTACTGGACAAGAGG+CGG 0.593495 4.2:-80504222 MS.gene78827:CDS
GCTGCATCAAGATAAGCTGC+TGG 0.594629 4.2:+80504781 None:intergenic
AACCCTAATGATTTCGTCGG+AGG 0.598673 4.2:-80503961 MS.gene78827:CDS
TGCAGAAGGTACTGGACAAG+AGG 0.603431 4.2:-80504225 MS.gene78827:CDS
CATGAGATGATAACTAGTGG+TGG 0.609089 4.2:-80503727 MS.gene78827:CDS
GATCGATGTCCGATAGGGCA+AGG 0.614474 4.2:-80504368 MS.gene78827:CDS
CAAGCTCGGACCGCCAAACG+GGG 0.615538 4.2:-80504107 MS.gene78827:CDS
AATCATGAGATGATAACTAG+TGG 0.640481 4.2:-80503730 MS.gene78827:CDS
ATGGAAGATAGTATCAATAG+GGG 0.643018 4.2:-80503643 MS.gene78827:CDS
TGTTGATGAGTCATTGACAT+TGG 0.643301 4.2:+80503826 None:intergenic
TGAGGTTGAGATTGTGACTG+TGG 0.644332 4.2:+80504423 None:intergenic
GGTTGAGATTGTGACTGTGG+TGG 0.650158 4.2:+80504426 None:intergenic
CATCAGCAACCAGGAAACGG+CGG 0.659295 4.2:-80504489 MS.gene78827:CDS
ACTTCTGCAAGACTTGCAGA+AGG 0.679196 4.2:-80504239 MS.gene78827:CDS
CCGGTGGATCCTCCTCCATG+AGG 0.687789 4.2:+80504405 None:intergenic
AAGGTACTGGACAAGAGGCG+GGG 0.703126 4.2:-80504220 MS.gene78827:CDS
TGTAAGGCCTAATATATCGG+CGG 0.708601 4.2:+80504035 None:intergenic
TAACTAGTGGTGGATATGTG+AGG 0.730675 4.2:-80503717 MS.gene78827:CDS
GTGTTGGTTGATTCACAACG+TGG 0.731087 4.2:+80504306 None:intergenic
TCACAATCTCAACCTCATGG+AGG 0.766876 4.2:-80504417 MS.gene78827:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTCAAAAGATAAAA+AGG - chr4.2:80503612-80503631 MS.gene78827:CDS 10.0%
!! TAAGAAAGAAATCAAATATT+TGG + chr4.2:80503685-80503704 None:intergenic 15.0%
!! AACAACATAAATAGACAAAA+AGG + chr4.2:80503760-80503779 None:intergenic 20.0%
!! ACAGGTAAATATAATTAGTA+AGG - chr4.2:80503542-80503561 MS.gene78827:CDS 20.0%
!! AGTAAGGTTTCAATTTAATT+TGG - chr4.2:80503558-80503577 MS.gene78827:CDS 20.0%
!! AGTATATGAATTGAGAATAA+TGG + chr4.2:80503719-80503738 None:intergenic 20.0%
!!! AATTCAATTTTATCATCAAG+TGG - chr4.2:80504420-80504439 MS.gene78827:CDS 20.0%
!!! TTTTGAATTATTTTGCCTTT+TGG + chr4.2:80503605-80503624 None:intergenic 20.0%
! AAATGGAAGATAGTATCAAT+AGG - chr4.2:80504643-80504662 MS.gene78827:intron 25.0%
! AATGGAAGATAGTATCAATA+GGG - chr4.2:80504644-80504663 MS.gene78827:intron 25.0%
! TATTTGGTATATTCAAGAAG+AGG + chr4.2:80503669-80503688 None:intergenic 25.0%
! TTACTGAAGTTATGATGTAA+AGG + chr4.2:80504309-80504328 None:intergenic 25.0%
! TTTGAACATTTCCTTCAAAA+GGG + chr4.2:80504539-80504558 None:intergenic 25.0%
!! AATTGAGAATAATGGAAGAA+AGG + chr4.2:80503711-80503730 None:intergenic 25.0%
!! AATTTTATCATCAAGTGGTT+TGG - chr4.2:80504425-80504444 MS.gene78827:CDS 25.0%
!! GAAGTAACAAAATTTTGTGT+TGG + chr4.2:80504001-80504020 None:intergenic 25.0%
!! TTTTGAACATTTCCTTCAAA+AGG + chr4.2:80504540-80504559 None:intergenic 25.0%
!!! AAACTATGGTTTTAGTTACA+TGG - chr4.2:80504362-80504381 MS.gene78827:CDS 25.0%
!!! AACTATGGTTTTAGTTACAT+GGG - chr4.2:80504363-80504382 MS.gene78827:CDS 25.0%
AACCTTAATGATGACATTTG+AGG + chr4.2:80504279-80504298 None:intergenic 30.0%
AATCATGAGATGATAACTAG+TGG - chr4.2:80504558-80504577 MS.gene78827:intron 30.0%
AATTAGCCAGAAAAGGAAAT+TGG + chr4.2:80504491-80504510 None:intergenic 30.0%
ATGGAAGATAGTATCAATAG+GGG - chr4.2:80504645-80504664 MS.gene78827:intron 30.0%
GGAAACAATAACAGTAATCA+AGG - chr4.2:80504687-80504706 MS.gene78827:intron 30.0%
! ATCAAGGAAGTGAACAATTT+TGG - chr4.2:80504703-80504722 MS.gene78827:intron 30.0%
!! AGTAATTTGTACCCTTTTGA+AGG - chr4.2:80504525-80504544 MS.gene78827:intron 30.0%
!! CTTTTGTTTCTTTGTCCAAA+AGG - chr4.2:80503587-80503606 MS.gene78827:CDS 30.0%
!!! ATTTCCTTTTCTGGCTAATT+TGG - chr4.2:80504491-80504510 MS.gene78827:CDS 30.0%
AATTGGACTGATCATGTTTC+TGG - chr4.2:80504759-80504778 MS.gene78827:intron 35.0%
AGGTGTAAGGCCTAATATAT+CGG + chr4.2:80504259-80504278 None:intergenic 35.0%
AGTAACCCTAATGATTTCGT+CGG - chr4.2:80504324-80504343 MS.gene78827:CDS 35.0%
CACCTCAAATGTCATCATTA+AGG - chr4.2:80504274-80504293 MS.gene78827:CDS 35.0%
CCAAAAGTGATTTCAACTTC+TGG - chr4.2:80504594-80504613 MS.gene78827:intron 35.0%
GGAAGAGTGGATATTGTTAT+TGG + chr4.2:80504235-80504254 None:intergenic 35.0%
TCAAGCAAAAGTAGCAACAA+TGG - chr4.2:80504138-80504157 MS.gene78827:CDS 35.0%
! AGAGTGGATATTGTTATTGG+AGG + chr4.2:80504232-80504251 None:intergenic 35.0%
! AGTATCAATAGGGGCTTTTT+GGG - chr4.2:80504654-80504673 MS.gene78827:intron 35.0%
! ATGATGACATTTGAGGTGTA+AGG + chr4.2:80504272-80504291 None:intergenic 35.0%
! CCAGAAGTTGAAATCACTTT+TGG + chr4.2:80504597-80504616 None:intergenic 35.0%
! GGAGATTTAGGGTTAAACTA+TGG - chr4.2:80504348-80504367 MS.gene78827:CDS 35.0%
! TAGTATCAATAGGGGCTTTT+TGG - chr4.2:80504653-80504672 MS.gene78827:intron 35.0%
! TCAACTTGCTTCTGTGAAAA+TGG - chr4.2:80504626-80504645 MS.gene78827:intron 35.0%
! TGGAATAGTGCTGTTATTAC+TGG - chr4.2:80504723-80504742 MS.gene78827:intron 35.0%
! TGTTGATGAGTCATTGACAT+TGG + chr4.2:80504465-80504484 None:intergenic 35.0%
!!! TATGGTTTTAGTTACATGGG+AGG - chr4.2:80504366-80504385 MS.gene78827:CDS 35.0%
ATCAAGATAAGCTGCTGGTA+TGG + chr4.2:80503505-80503524 None:intergenic 40.0%
ATTTCGTCGGAGGAGATTTA+GGG - chr4.2:80504337-80504356 MS.gene78827:CDS 40.0%
CATGAGATGATAACTAGTGG+TGG - chr4.2:80504561-80504580 MS.gene78827:intron 40.0%
CTGGTAATTCTGCAGCTAAT+TGG - chr4.2:80504742-80504761 MS.gene78827:intron 40.0%
TGGAAACTCAACAAAGTCTC+CGG - chr4.2:80504158-80504177 MS.gene78827:CDS 40.0%
TGTAAGGCCTAATATATCGG+CGG + chr4.2:80504256-80504275 None:intergenic 40.0%
TTTAGTTACATGGGAGGAGT+AGG - chr4.2:80504372-80504391 MS.gene78827:CDS 40.0%
! ACAGCACCAATTTCCTTTTC+TGG - chr4.2:80504482-80504501 MS.gene78827:CDS 40.0%
!! TAACTAGTGGTGGATATGTG+AGG - chr4.2:80504571-80504590 MS.gene78827:intron 40.0%
!!! CCTTTTCTGGCTAATTTGGA+GGG - chr4.2:80504495-80504514 MS.gene78827:CDS 40.0%
!!! TCCTTTTCTGGCTAATTTGG+AGG - chr4.2:80504494-80504513 MS.gene78827:CDS 40.0%
AACCCTAATGATTTCGTCGG+AGG - chr4.2:80504327-80504346 MS.gene78827:CDS 45.0%
ACTTCTGCAAGACTTGCAGA+AGG - chr4.2:80504049-80504068 MS.gene78827:CDS 45.0%
ATGGGAGGAGTAGGTGATTT+GGG - chr4.2:80504381-80504400 MS.gene78827:CDS 45.0%
ATTTACCTGTTGCTGCCAGT+TGG + chr4.2:80503532-80503551 None:intergenic 45.0%
CACTCTTCCGCCGATATATT+AGG - chr4.2:80504246-80504265 MS.gene78827:CDS 45.0%
CAGAATCATCATCAGCAACC+AGG - chr4.2:80503790-80503809 MS.gene78827:CDS 45.0%
CAGTCACAATCTCAACCTCA+TGG - chr4.2:80503868-80503887 MS.gene78827:CDS 45.0%
CATCATCAGCAACCAGGAAA+CGG - chr4.2:80503796-80503815 MS.gene78827:CDS 45.0%
CCCTCCAAATTAGCCAGAAA+AGG + chr4.2:80504498-80504517 None:intergenic 45.0%
CTAATATATCGGCGGAAGAG+TGG + chr4.2:80504248-80504267 None:intergenic 45.0%
CTCCTCCGACGAAATCATTA+GGG + chr4.2:80504332-80504351 None:intergenic 45.0%
CTGAAGCAGTTGTTGAGAGA+CGG + chr4.2:80503827-80503846 None:intergenic 45.0%
CTTATCTTGATGCAGCCAAC+TGG - chr4.2:80503514-80503533 MS.gene78827:CDS 45.0%
GATTTCGTCGGAGGAGATTT+AGG - chr4.2:80504336-80504355 MS.gene78827:CDS 45.0%
TCACAATCTCAACCTCATGG+AGG - chr4.2:80503871-80503890 MS.gene78827:CDS 45.0%
TCTCCTCCGACGAAATCATT+AGG + chr4.2:80504333-80504352 None:intergenic 45.0%
TGAGGTTGAGATTGTGACTG+TGG + chr4.2:80503868-80503887 None:intergenic 45.0%
! CATCAAGTGGTTTGGAGCAA+TGG - chr4.2:80504433-80504452 MS.gene78827:CDS 45.0%
!! CAAGTCTTGCAGAAGTGTCT+GGG + chr4.2:80504045-80504064 None:intergenic 45.0%
!! GATTTGGGAAGTGCTTTAGG+AGG - chr4.2:80504396-80504415 MS.gene78827:CDS 45.0%
!! GGCTTTTTGGGAATTAGCAC+AGG - chr4.2:80504666-80504685 MS.gene78827:intron 45.0%
!! GGTGATTTGGGAAGTGCTTT+AGG - chr4.2:80504393-80504412 MS.gene78827:CDS 45.0%
!! GTGTTGGTTGATTCACAACG+TGG + chr4.2:80503985-80504004 None:intergenic 45.0%
AACAAAGTCTCCGGCAAGCT+CGG - chr4.2:80504167-80504186 MS.gene78827:CDS 50.0%
CAATCTCAACCTCATGGAGG+AGG - chr4.2:80503874-80503893 MS.gene78827:CDS 50.0%
CATGGGAGGAGTAGGTGATT+TGG - chr4.2:80504380-80504399 MS.gene78827:CDS 50.0%
GCTGCATCAAGATAAGCTGC+TGG + chr4.2:80503510-80503529 None:intergenic 50.0%
GGTTGAGATTGTGACTGTGG+TGG + chr4.2:80503865-80503884 None:intergenic 50.0%
TGAGATTGTGACTGTGGTGG+TGG + chr4.2:80503862-80503881 None:intergenic 50.0%
TTGAACTAGCACTCCCCGTT+TGG + chr4.2:80504197-80504216 None:intergenic 50.0%
! CAAGTGGTTTGGAGCAATGG+AGG - chr4.2:80504436-80504455 MS.gene78827:CDS 50.0%
! GCAAGACTTGCAGAAGGTAC+TGG - chr4.2:80504055-80504074 MS.gene78827:CDS 50.0%
!! AGAAGGTACTGGACAAGAGG+CGG - chr4.2:80504066-80504085 MS.gene78827:CDS 50.0%
!! GCAAGTCTTGCAGAAGTGTC+TGG + chr4.2:80504046-80504065 None:intergenic 50.0%
!! TGCAGAAGGTACTGGACAAG+AGG - chr4.2:80504063-80504082 MS.gene78827:CDS 50.0%
AACTAGCACTCCCCGTTTGG+CGG + chr4.2:80504194-80504213 None:intergenic 55.0%
CATCAGCAACCAGGAAACGG+CGG - chr4.2:80503799-80503818 MS.gene78827:CDS 55.0%
CGATCCTGGCCTAATTGAGC+CGG + chr4.2:80503905-80503924 None:intergenic 55.0%
GATCGATGTCCGATAGGGCA+AGG - chr4.2:80503920-80503939 MS.gene78827:CDS 55.0%
GATGGCGAACATGCCTATGC+CGG - chr4.2:80503942-80503961 MS.gene78827:CDS 55.0%
GATGTCCGATAGGGCAAGGA+TGG - chr4.2:80503924-80503943 MS.gene78827:CDS 55.0%
GCCAGGATCGATGTCCGATA+GGG - chr4.2:80503915-80503934 MS.gene78827:CDS 55.0%
GGACATTTCAGTGCTGCCTC+CGG + chr4.2:80503964-80503983 None:intergenic 55.0%
GTTCGCCATCCTTGCCCTAT+CGG + chr4.2:80503932-80503951 None:intergenic 55.0%
!! AAGGTACTGGACAAGAGGCG+GGG - chr4.2:80504068-80504087 MS.gene78827:CDS 55.0%
!! GAAGGTACTGGACAAGAGGC+GGG - chr4.2:80504067-80504086 MS.gene78827:CDS 55.0%
!! GAGGATCCACCGGCTCAATT+AGG - chr4.2:80503893-80503912 MS.gene78827:CDS 55.0%
!! GATTGTGACTGTGGTGGTGG+TGG + chr4.2:80503859-80503878 None:intergenic 55.0%
!! GCTCTAAGGAATGTCCCGGT+CGG - chr4.2:80504090-80504109 MS.gene78827:CDS 55.0%
CCTCATGGAGGAGGATCCAC+CGG - chr4.2:80503883-80503902 MS.gene78827:CDS 60.0%
CTAAGGAATGTCCCGGTCGG+TGG - chr4.2:80504093-80504112 MS.gene78827:CDS 60.0%
GCCCTATCGGACATCGATCC+TGG + chr4.2:80503919-80503938 None:intergenic 60.0%
GGCCAGGATCGATGTCCGAT+AGG - chr4.2:80503914-80503933 MS.gene78827:CDS 60.0%
TCCTGGCCTAATTGAGCCGG+TGG + chr4.2:80503902-80503921 None:intergenic 60.0%
TGCAGCCAACTGGCAGCAAC+AGG - chr4.2:80503524-80503543 MS.gene78827:CDS 60.0%
TTCAGTGCTGCCTCCGGCAT+AGG + chr4.2:80503958-80503977 None:intergenic 60.0%
!! CGGGGCTCTAAGGAATGTCC+CGG - chr4.2:80504086-80504105 MS.gene78827:CDS 60.0%
!! TCCACCGGCTCAATTAGGCC+AGG - chr4.2:80503898-80503917 MS.gene78827:CDS 60.0%
!! TGTGACTGTGGTGGTGGTGG+TGG + chr4.2:80503856-80503875 None:intergenic 60.0%
AGGAATGTCCCGGTCGGTGG+AGG - chr4.2:80504096-80504115 MS.gene78827:CDS 65.0%
CAAGCTCGGACCGCCAAACG+GGG - chr4.2:80504181-80504200 MS.gene78827:CDS 65.0%
CCGGTGGATCCTCCTCCATG+AGG + chr4.2:80503886-80503905 None:intergenic 65.0%
CTCCGAAAGCCTCCACCGAC+CGG + chr4.2:80504108-80504127 None:intergenic 65.0%
GCAAGCTCGGACCGCCAAAC+GGG - chr4.2:80504180-80504199 MS.gene78827:CDS 65.0%
GGACAAGAGGCGGGGCTCTA+AGG - chr4.2:80504076-80504095 MS.gene78827:CDS 65.0%
GGCAAGCTCGGACCGCCAAA+CGG - chr4.2:80504179-80504198 MS.gene78827:CDS 65.0%
GGCGAACATGCCTATGCCGG+AGG - chr4.2:80503945-80503964 MS.gene78827:CDS 65.0%
GTTTGGCGGTCCGAGCTTGC+CGG + chr4.2:80504180-80504199 None:intergenic 65.0%
TCCCGGTCGGTGGAGGCTTT+CGG - chr4.2:80504103-80504122 MS.gene78827:CDS 65.0%
TCCGAAAGCCTCCACCGACC+GGG + chr4.2:80504107-80504126 None:intergenic 65.0%
!! GACTGTGGTGGTGGTGGTGG+TGG + chr4.2:80503853-80503872 None:intergenic 65.0%
GAGACGGAGCCGCCGTTTCC+TGG + chr4.2:80503811-80503830 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 80503489 80504821 80503489 ID=MS.gene78827
chr4.2 mRNA 80503489 80504821 80503489 ID=MS.gene78827.t1;Parent=MS.gene78827
chr4.2 exon 80504765 80504821 80504765 ID=MS.gene78827.t1.exon1;Parent=MS.gene78827.t1
chr4.2 CDS 80504765 80504821 80504765 ID=cds.MS.gene78827.t1;Parent=MS.gene78827.t1
chr4.2 exon 80503489 80504523 80503489 ID=MS.gene78827.t1.exon2;Parent=MS.gene78827.t1
chr4.2 CDS 80503489 80504523 80503489 ID=cds.MS.gene78827.t1;Parent=MS.gene78827.t1
Gene Sequence

>MS.gene78827

ATGGTTTTTACTTCCATACCAGCAGCTTATCTTGATGCAGCCAACTGGCAGCAACAGGTAAATATAATTAGTAAGGTTTCAATTTAATTTGGTTCTTGCTTTTGTTTCTTTGTCCAAAAGGCAAAATAATTCAAAAGATAAAAAGGAAAAACAATATTCTTGTTACTTTTATTTATACCTCTTCTTGAATATACCAAATATTTGATTTCTTTCTTATTACCTTTCTTCCATTATTCTCAATTCATATACTCTTTTTCTAATTTTTCTTCCTTTTTGTCTATTTATGTTGTTATTTCAGCAACAGAATCATCATCAGCAACCAGGAAACGGCGGCTCCGTCTCTCAACAACTGCTTCAGACACCACCACCACCACCACCACAGTCACAATCTCAACCTCATGGAGGAGGATCCACCGGCTCAATTAGGCCAGGATCGATGTCCGATAGGGCAAGGATGGCGAACATGCCTATGCCGGAGGCAGCACTGAAATGTCCACGTTGTGAATCAACCAACACAAAATTTTGTTACTTCAACAACTACAGTCTCTCTCAGCCCAGACACTTCTGCAAGACTTGCAGAAGGTACTGGACAAGAGGCGGGGCTCTAAGGAATGTCCCGGTCGGTGGAGGCTTTCGGAGAAACAAGAGATCAAGCAAAAGTAGCAACAATGGAAACTCAACAAAGTCTCCGGCAAGCTCGGACCGCCAAACGGGGAGTGCTAGTTCAACAAACTCAATCACCTCCAATAACAATATCCACTCTTCCGCCGATATATTAGGCCTTACACCTCAAATGTCATCATTAAGGTTCATGACACCTTTACATCATAACTTCAGTAACCCTAATGATTTCGTCGGAGGAGATTTAGGGTTAAACTATGGTTTTAGTTACATGGGAGGAGTAGGTGATTTGGGAAGTGCTTTAGGAGGTAATTCAATTTTATCATCAAGTGGTTTGGAGCAATGGAGGATGCCAATGTCAATGACTCATCAACAGCACCAATTTCCTTTTCTGGCTAATTTGGAGGGTTCAAATAGTAATTTGTACCCTTTTGAAGGAAATGTTCAAAATCATGAGATGATAACTAGTGGTGGATATGTGAGGCCAAAAGTGATTTCAACTTCTGGAATTATGACTCAACTTGCTTCTGTGAAAATGGAAGATAGTATCAATAGGGGCTTTTTGGGAATTAGCACAGGAAACAATAACAGTAATCAAGGAAGTGAACAATTTTGGAATAGTGCTGTTATTACTGGTAATTCTGCAGCTAATTGGACTGATCATGTTTCTGGTTTTAGCTCTTCTTCTAATACTACTAGTAACCAGATGTAG
Protein sequence

>MS.gene78827.t1

MVFTSIPAAYLDAANWQQQQQNHHQQPGNGGSVSQQLLQTPPPPPPQSQSQPHGGGSTGSIRPGSMSDRARMANMPMPEAALKCPRCESTNTKFCYFNNYSLSQPRHFCKTCRRYWTRGGALRNVPVGGGFRRNKRSSKSSNNGNSTKSPASSDRQTGSASSTNSITSNNNIHSSADILGLTPQMSSLRFMTPLHHNFSNPNDFVGGDLGLNYGFSYMGGVGDLGSALGGNSILSSSGLEQWRMPMSMTHQQHQFPFLANLEGSNSNLYPFEGNVQNHEMITSGGYVRPKVISTSGIMTQLASVKMEDSINRGFLGISTGNNNSNQGSEQFWNSAVITGNSAANWTDHVSGFSSSSNTTSNQM