AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79191

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79191.t1 MTR_7g029400 95.833 192 7 1 1 191 1 192 1.44e-136 379
MS.gene79191.t1 MTR_8g015250 89.529 191 10 1 1 191 1 181 8.64e-124 347
MS.gene79191.t1 MTR_6g016375 62.121 132 42 1 30 161 44 167 1.16e-45 149
MS.gene79191.t1 MTR_7g017880 60.000 140 52 3 50 187 53 190 6.64e-44 145
MS.gene79191.t1 MTR_4g070860 52.055 73 35 0 80 152 26 98 4.43e-18 77.4
MS.gene79191.t1 MTR_1g022495 42.553 94 53 1 61 154 8 100 3.46e-17 75.1
MS.gene79191.t1 MTR_3g117120 49.315 73 37 0 80 152 26 98 1.84e-15 70.5
MS.gene79191.t1 MTR_4g097440 34.646 127 71 2 42 156 21 147 2.91e-15 70.5
MS.gene79191.t1 MTR_5g015090 42.708 96 48 1 81 169 58 153 7.21e-15 68.9
MS.gene79191.t1 MTR_8g086020 34.375 96 63 0 57 152 8 103 1.18e-12 63.2
MS.gene79191.t1 MTR_1g087040 42.391 92 47 3 61 152 10 95 2.13e-12 62.4
MS.gene79191.t1 MTR_5g028850 39.362 94 52 2 59 147 280 373 1.51e-11 62.4
MS.gene79191.t1 MTR_5g028850 39.362 94 52 2 59 147 278 371 1.74e-11 62.4
MS.gene79191.t1 MTR_3g103360 36.283 113 68 1 47 159 23 131 7.35e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79191.t1 AT3G30530 68.103 116 35 1 62 175 57 172 4.62e-49 157
MS.gene79191.t1 AT1G13600 55.319 141 51 4 35 171 45 177 4.08e-40 135
MS.gene79191.t1 AT5G38800 63.208 106 39 0 61 166 49 154 8.49e-40 133
MS.gene79191.t1 AT5G15830 57.983 119 42 3 61 172 52 169 1.23e-35 123
MS.gene79191.t1 AT2G04038 59.459 111 41 1 65 171 55 165 4.55e-35 121
MS.gene79191.t1 AT1G68880 45.455 99 52 1 63 161 28 124 2.04e-22 88.2
MS.gene79191.t1 AT1G75390 46.809 94 45 1 64 152 16 109 4.66e-17 75.1
MS.gene79191.t1 AT5G60830 48.718 78 40 0 82 159 69 146 5.71e-16 73.2
MS.gene79191.t1 AT1G75390 47.059 85 41 1 62 142 15 99 6.45e-16 70.9
MS.gene79191.t1 AT2G18160 47.222 72 38 0 81 152 28 99 1.57e-14 68.6
MS.gene79191.t1 AT3G62420 37.624 101 59 1 80 176 21 121 1.16e-12 63.2
MS.gene79191.t1 AT4G37730 47.619 84 44 0 76 159 189 272 2.90e-12 64.3
MS.gene79191.t1 AT4G34590 43.243 74 42 0 79 152 22 95 7.72e-12 61.2

Find 28 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTATTTAGATGGTAATT+TGG 0.228192 7.2:+68630707 None:intergenic
TTCCTAATAGATTATTTAGA+TGG 0.311060 7.2:+68630699 None:intergenic
AATTGATCATGGCCTGCTAA+TGG 0.346031 7.2:+68630656 None:intergenic
TACCATCTAAATAATCTATT+AGG 0.346268 7.2:-68630701 MS.gene79191:CDS
GCTAATGGGTAGTGGAAGTT+TGG 0.389519 7.2:+68630671 None:intergenic
ATGGTAGCTGACATGCAAAT+TGG 0.415880 7.2:-68630341 MS.gene79191:CDS
ATTGATCATGGCCTGCTAAT+GGG 0.427113 7.2:+68630657 None:intergenic
ACTGAAAATCACAACCTTGT+TGG 0.430174 7.2:-68630449 MS.gene79191:CDS
TTTGCATGTCAGCTACCATT+TGG 0.441661 7.2:+68630345 None:intergenic
CATGGTTTAGTTTGCCAACA+AGG 0.492975 7.2:+68630435 None:intergenic
GAGAATCAGCTAGGAGATCA+AGG 0.496394 7.2:-68630526 MS.gene79191:CDS
AACTTCCACTACCCATTAGC+AGG 0.516381 7.2:-68630668 MS.gene79191:CDS
CATAAATCTTCCATGTTGCA+AGG 0.518307 7.2:+68630308 None:intergenic
TAATTTGGAATTTCATTCTG+TGG 0.545612 7.2:+68630722 None:intergenic
CAGCTAGGAGATCAAGGATG+AGG 0.553553 7.2:-68630520 MS.gene79191:CDS
CATGGCCTGCTAATGGGTAG+TGG 0.565162 7.2:+68630663 None:intergenic
TTGTGTTGAGATGTGTTGCA+TGG 0.567617 7.2:+68630278 None:intergenic
AAGGTGGAGCAAATTGATCA+TGG 0.575829 7.2:+68630645 None:intergenic
GAGTTGCTGCTTAAACAAGA+AGG 0.605858 7.2:+68630626 None:intergenic
TTGACTCCTCTCATCTCACT+AGG 0.610669 7.2:+68630797 None:intergenic
AGTTCAACATCATCGACGAA+AGG 0.627624 7.2:-68630574 MS.gene79191:CDS
TATCGAATAGAGAATCAGCT+AGG 0.637053 7.2:-68630535 MS.gene79191:CDS
TTGCTGCTTAAACAAGAAGG+TGG 0.640187 7.2:+68630629 None:intergenic
TCTCATCTCACTAGGAACCA+TGG 0.643297 7.2:+68630805 None:intergenic
TCATCGACGAAAGGAAGCAT+CGG 0.644628 7.2:-68630565 MS.gene79191:CDS
TGTCATGTGACTCAGACACA+TGG 0.644643 7.2:+68630417 None:intergenic
GGATGAACTTTGGTCACAAG+TGG 0.651183 7.2:-68630483 MS.gene79191:CDS
ATGGTTCCTAGTGAGATGAG+AGG 0.683863 7.2:-68630803 MS.gene79191:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATTATTTAGATGGTAATT+TGG + chr7.2:68630349-68630368 None:intergenic 20.0%
!! TACCATCTAAATAATCTATT+AGG - chr7.2:68630352-68630371 MS.gene79191:CDS 20.0%
!! TTCCTAATAGATTATTTAGA+TGG + chr7.2:68630357-68630376 None:intergenic 20.0%
!! TAATTTGGAATTTCATTCTG+TGG + chr7.2:68630334-68630353 None:intergenic 25.0%
AAAAACACTTGGATGAACTT+TGG - chr7.2:68630560-68630579 MS.gene79191:CDS 30.0%
!! ACATGGAATGAATTTTCAAG+AGG + chr7.2:68630292-68630311 None:intergenic 30.0%
!!! AGACAAAGTTTTGTTGAACA+TGG + chr7.2:68630309-68630328 None:intergenic 30.0%
ACTGAAAATCACAACCTTGT+TGG - chr7.2:68630604-68630623 MS.gene79191:CDS 35.0%
CATAAATCTTCCATGTTGCA+AGG + chr7.2:68630748-68630767 None:intergenic 35.0%
GATGAGGAAACAAAAACACT+TGG - chr7.2:68630549-68630568 MS.gene79191:CDS 35.0%
TATCGAATAGAGAATCAGCT+AGG - chr7.2:68630518-68630537 MS.gene79191:CDS 35.0%
! AACTTTTGATCTTCGCCAAA+TGG - chr7.2:68630693-68630712 MS.gene79191:CDS 35.0%
! TAACTCTTTTCCTTGCAACA+TGG - chr7.2:68630735-68630754 MS.gene79191:CDS 35.0%
AAGGTGGAGCAAATTGATCA+TGG + chr7.2:68630411-68630430 None:intergenic 40.0%
AATTGATCATGGCCTGCTAA+TGG + chr7.2:68630400-68630419 None:intergenic 40.0%
AGTTCAACATCATCGACGAA+AGG - chr7.2:68630479-68630498 MS.gene79191:CDS 40.0%
ATGGTAGCTGACATGCAAAT+TGG - chr7.2:68630712-68630731 MS.gene79191:CDS 40.0%
ATTGATCATGGCCTGCTAAT+GGG + chr7.2:68630399-68630418 None:intergenic 40.0%
TTGTGTTGAGATGTGTTGCA+TGG + chr7.2:68630778-68630797 None:intergenic 40.0%
TTTGCATGTCAGCTACCATT+TGG + chr7.2:68630711-68630730 None:intergenic 40.0%
! GAGTTGCTGCTTAAACAAGA+AGG + chr7.2:68630430-68630449 None:intergenic 40.0%
! TTGCTGCTTAAACAAGAAGG+TGG + chr7.2:68630427-68630446 None:intergenic 40.0%
!! CATGGTTTAGTTTGCCAACA+AGG + chr7.2:68630621-68630640 None:intergenic 40.0%
AACTTCCACTACCCATTAGC+AGG - chr7.2:68630385-68630404 MS.gene79191:CDS 45.0%
ATGGTTCCTAGTGAGATGAG+AGG - chr7.2:68630250-68630269 MS.gene79191:CDS 45.0%
GAGAATCAGCTAGGAGATCA+AGG - chr7.2:68630527-68630546 MS.gene79191:CDS 45.0%
GGATGAACTTTGGTCACAAG+TGG - chr7.2:68630570-68630589 MS.gene79191:CDS 45.0%
TGTCATGTGACTCAGACACA+TGG + chr7.2:68630639-68630658 None:intergenic 45.0%
TTGACTCCTCTCATCTCACT+AGG + chr7.2:68630259-68630278 None:intergenic 45.0%
! TCATCGACGAAAGGAAGCAT+CGG - chr7.2:68630488-68630507 MS.gene79191:CDS 45.0%
!! GCTAATGGGTAGTGGAAGTT+TGG + chr7.2:68630385-68630404 None:intergenic 45.0%
CAGCTAGGAGATCAAGGATG+AGG - chr7.2:68630533-68630552 MS.gene79191:CDS 50.0%
! CATGGCCTGCTAATGGGTAG+TGG + chr7.2:68630393-68630412 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 68630250 68630825 68630250 ID=MS.gene79191
chr7.2 mRNA 68630250 68630825 68630250 ID=MS.gene79191.t1;Parent=MS.gene79191
chr7.2 exon 68630250 68630825 68630250 ID=MS.gene79191.t1.exon1;Parent=MS.gene79191.t1
chr7.2 CDS 68630250 68630825 68630250 ID=cds.MS.gene79191.t1;Parent=MS.gene79191.t1
Gene Sequence

>MS.gene79191

ATGGTTCCTAGTGAGATGAGAGGAGTCAATTATCTAACTCCTCTTGAAAATTCATTCCATGTTCAACAAAACTTTGTCTTGCCACAGAATGAAATTCCAAATTACCATCTAAATAATCTATTAGGAAGCTTGCCAAACTTCCACTACCCATTAGCAGGCCATGATCAATTTGCTCCACCTTCTTGTTTAAGCAGCAACTCAACTACTTCTGATGAAGCAGATGAACTTCAGTTCAACATCATCGACGAAAGGAAGCATCGGCGAATGATATCGAATAGAGAATCAGCTAGGAGATCAAGGATGAGGAAACAAAAACACTTGGATGAACTTTGGTCACAAGTGGTGAGACTGAGAACTGAAAATCACAACCTTGTTGGCAAACTAAACCATGTGTCTGAGTCACATGACAAAGTTGTTCAAGAGAATATACGTCTTAAAGAAGAAACTTTTGATCTTCGCCAAATGGTAGCTGACATGCAAATTGGTAACTCTTTTCCTTGCAACATGGAAGATTTATGTGAAATTCCATGCAACACATCTCAACACAAAGATGATTCTTCTAAAATTCATGATTGA
Protein sequence

>MS.gene79191.t1

MVPSEMRGVNYLTPLENSFHVQQNFVLPQNEIPNYHLNNLLGSLPNFHYPLAGHDQFAPPSCLSSNSTTSDEADELQFNIIDERKHRRMISNRESARRSRMRKQKHLDELWSQVVRLRTENHNLVGKLNHVSESHDKVVQENIRLKEETFDLRQMVADMQIGNSFPCNMEDLCEIPCNTSQHKDDSSKIHD