AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79454

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79454.t1 MTR_8g099880 95.113 266 9 1 1 266 66 327 2.30e-166 463
MS.gene79454.t1 MTR_3g498825 60.886 271 89 7 1 266 72 330 8.46e-94 280
MS.gene79454.t1 MTR_7g053410 50.532 188 67 7 22 187 234 417 2.41e-45 159
MS.gene79454.t1 MTR_7g053410 50.532 188 67 7 22 187 234 417 4.46e-45 159
MS.gene79454.t1 MTR_5g037250 54.305 151 59 2 14 155 34 183 1.71e-43 148
MS.gene79454.t1 MTR_1g017350 50.602 166 71 4 12 171 184 344 2.57e-43 152
MS.gene79454.t1 MTR_1g017350 50.602 166 71 4 12 171 184 344 3.17e-43 152
MS.gene79454.t1 MTR_1g017350 50.602 166 71 4 12 171 184 344 3.43e-43 152
MS.gene79454.t1 MTR_1g017350 50.602 166 71 4 12 171 184 344 4.05e-43 152
MS.gene79454.t1 MTR_8g065740 61.719 128 43 2 37 164 263 384 5.72e-42 149
MS.gene79454.t1 MTR_8g012290 65.546 119 36 2 55 173 264 377 1.82e-41 149
MS.gene79454.t1 MTR_8g012290 65.546 119 36 2 55 173 264 377 1.99e-41 149
MS.gene79454.t1 MTR_3g116770 82.558 86 14 1 79 164 186 270 5.60e-41 145
MS.gene79454.t1 MTR_3g116770 82.558 86 14 1 79 164 186 270 5.76e-41 145
MS.gene79454.t1 MTR_8g062240 55.479 146 53 2 22 155 77 222 9.23e-41 142
MS.gene79454.t1 MTR_5g048860 76.289 97 19 3 81 176 362 455 5.05e-40 145
MS.gene79454.t1 MTR_4g070320 82.143 84 14 1 81 164 140 222 5.50e-40 142
MS.gene79454.t1 MTR_1g059270 69.388 98 25 3 73 170 151 243 1.17e-39 138
MS.gene79454.t1 MTR_1g059270 66.667 102 29 3 69 170 147 243 4.02e-39 139
MS.gene79454.t1 MTR_7g092510 61.667 120 37 4 81 195 170 285 1.02e-37 135
MS.gene79454.t1 MTR_6g084120 46.212 132 47 4 40 168 122 232 1.93e-26 104
MS.gene79454.t1 MTR_4g131160 57.831 83 30 2 84 162 196 277 3.34e-21 92.0
MS.gene79454.t1 MTR_4g131160 57.831 83 30 2 84 162 199 280 3.48e-21 92.0
MS.gene79454.t1 MTR_1g080890 65.152 66 22 1 84 149 193 257 2.63e-19 86.3
MS.gene79454.t1 MTR_4g033295 55.385 65 29 0 84 148 118 182 1.05e-15 73.9
MS.gene79454.t1 MTR_4g033295 53.731 67 30 1 84 149 118 184 2.18e-15 72.8
MS.gene79454.t1 MTR_4g065870 56.923 65 28 0 84 148 355 419 3.95e-15 75.1
MS.gene79454.t1 MTR_1g069155 37.190 121 62 4 39 159 284 390 1.17e-14 73.9
MS.gene79454.t1 MTR_4g065870 56.452 62 27 0 84 145 355 416 2.14e-14 72.8
MS.gene79454.t1 MTR_7g099540 31.677 161 93 4 55 209 224 373 3.99e-14 72.0
MS.gene79454.t1 MTR_1g084980 38.202 89 54 1 61 149 439 526 8.80e-14 71.2
MS.gene79454.t1 MTR_2g098310 50.746 67 33 0 82 148 330 396 9.08e-14 70.9
MS.gene79454.t1 MTR_1g052470 50.725 69 34 0 84 152 298 366 1.09e-13 70.5
MS.gene79454.t1 MTR_1g070870 38.732 142 72 3 36 163 62 202 1.23e-13 69.7
MS.gene79454.t1 MTR_7g092540 46.154 78 39 1 75 152 237 311 1.59e-13 69.7
MS.gene79454.t1 MTR_2g098310 51.515 66 32 0 82 147 330 395 2.71e-13 69.3
MS.gene79454.t1 MTR_1g070870 51.190 84 36 2 84 163 123 205 7.13e-13 67.4
MS.gene79454.t1 MTR_7g092540 45.946 74 37 1 75 148 237 307 1.21e-12 67.0
MS.gene79454.t1 MTR_5g017040 38.596 114 62 4 53 159 68 180 1.26e-12 67.4
MS.gene79454.t1 MTR_3g028520 49.231 65 33 0 84 148 322 386 4.34e-12 65.9
MS.gene79454.t1 MTR_3g027650 49.231 65 33 0 84 148 322 386 4.34e-12 65.9
MS.gene79454.t1 MTR_7g099540 30.539 167 93 5 55 209 224 379 6.60e-12 65.5
MS.gene79454.t1 MTR_4g088710 55.357 56 22 2 214 266 12 67 9.66e-12 59.7
MS.gene79454.t1 MTR_1g018090 49.231 65 32 1 84 148 274 337 1.28e-11 64.3
MS.gene79454.t1 MTR_3g027650 50.000 62 31 0 84 145 322 383 1.96e-11 63.9
MS.gene79454.t1 MTR_3g449770 33.094 139 67 5 86 212 311 435 2.86e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79454.t1 AT5G50915 66.406 128 39 1 44 171 100 223 2.48e-55 179
MS.gene79454.t1 AT5G50915 66.406 128 39 1 44 171 100 223 2.48e-55 179
MS.gene79454.t1 AT5G50915 66.406 128 39 1 44 171 100 223 5.48e-55 179
MS.gene79454.t1 AT5G50915 66.406 128 39 1 44 171 100 223 5.48e-55 179
MS.gene79454.t1 AT3G23690 46.018 226 87 7 30 244 132 333 6.52e-47 160
MS.gene79454.t1 AT1G26260 54.658 161 53 5 40 189 117 268 3.67e-46 157
MS.gene79454.t1 AT1G26260 54.658 161 53 5 40 189 146 297 7.50e-46 157
MS.gene79454.t1 AT1G26260 54.658 161 53 5 40 189 168 319 9.85e-46 158
MS.gene79454.t1 AT1G26260 54.658 161 53 5 40 189 168 319 9.85e-46 158
MS.gene79454.t1 AT1G26260 54.658 161 53 5 40 189 168 319 9.85e-46 158
MS.gene79454.t1 AT1G26260 54.658 161 53 5 40 189 168 319 9.85e-46 158
MS.gene79454.t1 AT1G68920 55.758 165 59 6 62 216 279 439 1.31e-45 159
MS.gene79454.t1 AT1G68920 55.758 165 59 6 62 216 280 440 1.50e-45 159
MS.gene79454.t1 AT1G68920 55.758 165 59 6 62 216 280 440 1.50e-45 159
MS.gene79454.t1 AT1G68920 55.758 165 59 6 62 216 280 440 1.50e-45 159
MS.gene79454.t1 AT1G10120 48.889 180 74 5 7 171 114 290 2.63e-44 154
MS.gene79454.t1 AT1G10120 48.889 180 74 5 7 171 114 290 2.63e-44 154
MS.gene79454.t1 AT3G07340 76.699 103 22 2 62 164 237 337 3.53e-44 155
MS.gene79454.t1 AT3G07340 76.699 103 22 2 62 164 237 337 3.82e-44 155
MS.gene79454.t1 AT5G48560 80.899 89 17 0 76 164 293 381 1.04e-42 151
MS.gene79454.t1 AT1G59640 55.862 145 63 1 12 155 63 207 1.22e-42 146
MS.gene79454.t1 AT5G48560 80.899 89 17 0 76 164 293 381 1.73e-42 151
MS.gene79454.t1 AT1G59640 66.957 115 38 0 41 155 93 207 3.03e-42 147
MS.gene79454.t1 AT4G34530 82.558 86 14 1 79 164 167 251 2.64e-41 145
MS.gene79454.t1 AT5G62610 53.425 146 51 3 42 170 101 246 9.72e-39 137
MS.gene79454.t1 AT5G62610 53.425 146 51 3 42 170 101 246 9.72e-39 137
MS.gene79454.t1 AT3G57800 71.875 96 22 3 75 170 197 287 1.56e-38 139
MS.gene79454.t1 AT2G42300 59.677 124 47 2 47 170 148 268 3.13e-38 135
MS.gene79454.t1 AT2G42300 59.677 124 47 2 47 170 148 268 1.53e-37 135
MS.gene79454.t1 AT2G18300 50.993 151 71 2 6 156 110 257 9.45e-37 133
MS.gene79454.t1 AT2G18300 50.993 151 71 2 6 156 110 257 9.95e-37 133
MS.gene79454.t1 AT4G36540 57.851 121 40 3 43 156 97 213 1.31e-36 132
MS.gene79454.t1 AT4G36540 57.851 121 40 3 43 156 97 213 1.40e-36 132
MS.gene79454.t1 AT2G18300 50.993 151 71 2 6 156 110 257 1.80e-36 133
MS.gene79454.t1 AT1G18400 67.816 87 27 1 82 168 143 228 5.46e-35 126
MS.gene79454.t1 AT1G18400 67.816 87 27 1 82 168 143 228 6.65e-35 126
MS.gene79454.t1 AT1G73830 67.442 86 27 1 83 168 146 230 7.32e-34 124
MS.gene79454.t1 AT1G73830 67.442 86 27 1 83 168 146 230 8.03e-34 123
MS.gene79454.t1 AT1G25330 61.053 95 32 1 78 172 98 187 1.79e-33 121
MS.gene79454.t1 AT3G57800 48.951 143 21 4 75 170 197 334 4.99e-30 117
MS.gene79454.t1 AT5G58010 50.962 104 38 3 84 183 99 193 3.06e-21 90.9
MS.gene79454.t1 AT2G24260 62.500 72 26 1 78 149 29 99 3.47e-21 89.7
MS.gene79454.t1 AT4G30980 43.590 117 52 2 33 149 92 194 9.80e-21 89.7
MS.gene79454.t1 AT4G30980 68.182 66 20 1 84 149 178 242 1.56e-20 90.1
MS.gene79454.t1 AT2G24260 62.500 72 26 1 78 149 132 202 2.46e-20 89.4
MS.gene79454.t1 AT2G42300 58.442 77 32 0 47 123 148 224 4.31e-19 84.0
MS.gene79454.t1 AT2G42300 58.442 77 32 0 47 123 148 224 4.31e-19 84.0
MS.gene79454.t1 AT2G42300 58.442 77 32 0 47 123 148 224 6.02e-19 83.6
MS.gene79454.t1 AT4G28800 45.833 72 38 1 87 158 252 322 8.45e-14 71.2
MS.gene79454.t1 AT4G28815 38.318 107 56 4 87 190 71 170 1.08e-13 68.9
MS.gene79454.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.16e-13 70.9
MS.gene79454.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.16e-13 70.9
MS.gene79454.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.16e-13 70.9
MS.gene79454.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.16e-13 70.9
MS.gene79454.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.16e-13 70.9
MS.gene79454.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.16e-13 70.9
MS.gene79454.t1 AT1G35460 50.769 65 32 0 84 148 181 245 1.81e-13 68.9
MS.gene79454.t1 AT1G51140 54.688 64 29 0 84 147 304 367 2.78e-13 69.3
MS.gene79454.t1 AT4G28815 38.318 107 56 4 87 190 147 246 3.81e-13 68.6
MS.gene79454.t1 AT2G20180 49.206 63 31 1 87 149 210 271 4.40e-13 68.9
MS.gene79454.t1 AT2G20180 49.206 63 31 1 87 149 210 271 4.40e-13 68.9
MS.gene79454.t1 AT2G20180 49.206 63 31 1 87 149 210 271 4.40e-13 68.9
MS.gene79454.t1 AT4G28800 42.169 83 44 2 87 166 264 345 4.63e-13 68.9
MS.gene79454.t1 AT2G20180 49.206 63 31 1 87 149 281 342 4.68e-13 68.9
MS.gene79454.t1 AT2G20180 49.206 63 31 1 87 149 281 342 4.68e-13 68.9
MS.gene79454.t1 AT2G20180 49.206 63 31 1 87 149 281 342 4.68e-13 68.9
MS.gene79454.t1 AT2G20180 49.206 63 31 1 87 149 281 342 4.68e-13 68.9
MS.gene79454.t1 AT2G42280 50.000 64 32 0 84 147 279 342 4.84e-13 68.6
MS.gene79454.t1 AT4G09180 49.254 67 34 0 82 148 182 248 5.37e-13 67.4
MS.gene79454.t1 AT2G42280 51.515 66 30 1 84 147 279 344 8.98e-13 67.8
MS.gene79454.t1 AT4G28790 36.735 98 58 2 62 159 252 345 9.17e-13 67.8
MS.gene79454.t1 AT4G02590 50.000 84 37 2 84 163 83 165 1.80e-12 65.9
MS.gene79454.t1 AT4G02590 50.000 84 37 2 84 163 146 228 2.91e-12 65.9
MS.gene79454.t1 AT4G02590 50.000 84 37 2 84 163 146 228 2.91e-12 65.9
MS.gene79454.t1 AT1G03040 59.091 66 26 1 84 149 139 203 1.02e-11 64.3
MS.gene79454.t1 AT1G03040 59.091 66 26 1 84 149 144 208 1.08e-11 64.3
MS.gene79454.t1 AT4G33880 45.783 83 42 3 84 165 266 346 1.35e-11 64.3
MS.gene79454.t1 AT1G03040 59.091 66 26 1 84 149 144 208 1.64e-11 63.9
MS.gene79454.t1 AT2G20180 49.153 59 29 1 87 145 281 338 1.78e-11 63.9
MS.gene79454.t1 AT2G43140 43.590 78 42 1 72 147 120 197 2.64e-11 62.0
MS.gene79454.t1 AT2G43140 43.590 78 42 1 72 147 217 294 3.32e-11 62.8
MS.gene79454.t1 AT4G00050 44.595 74 40 1 75 148 96 168 4.83e-11 62.4

Find 62 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAATTGATATTAATTTA+AGG 0.098761 4.1:+6573444 None:intergenic
AGCACACTGATGGACCATTT+TGG 0.191470 4.1:-6573528 MS.gene79454:CDS
TGTTAATAATGACTATGTTT+TGG 0.279340 4.1:-6574207 MS.gene79454:CDS
TCAGAAAGCTGTTACTTCTA+TGG 0.295160 4.1:-6575580 MS.gene79454:CDS
TTTCATATTAAATAGGTAAC+TGG 0.313052 4.1:-6574692 MS.gene79454:intron
TTTGAGTTGCCCCTTGTCTA+AGG 0.332895 4.1:-6575526 MS.gene79454:intron
CAAAAGCTTCTTAATCCATA+TGG 0.364693 4.1:-6573487 MS.gene79454:CDS
ATTGCAAAGTCAAAGAAGAT+TGG 0.375639 4.1:+6574484 None:intergenic
TATTTCCTCACCTGATGATC+TGG 0.386280 4.1:+6574436 None:intergenic
AAAACATAGTCATTATTAAC+AGG 0.386747 4.1:+6574209 None:intergenic
CTTCTTAATCCATATGGATT+CGG 0.417843 4.1:-6573481 MS.gene79454:CDS
AACTTGTGCCTGGCTGTGAT+AGG 0.422109 4.1:-6574930 MS.gene79454:CDS
TAGCCACAGCCTTGCTGAAA+GGG 0.444848 4.1:-6575242 MS.gene79454:intron
ACATGTGGTATTGGTGTTGA+TGG 0.451696 4.1:+6574293 None:intergenic
CACAATGGTACATGTGGTAT+TGG 0.457363 4.1:+6574284 None:intergenic
TGCTCTTCTACGTCCCAAAA+TGG 0.463393 4.1:+6573514 None:intergenic
TTGTAGGATGTCTCAAAAGA+AGG 0.474006 4.1:-6575412 MS.gene79454:intron
GTGTTGTTGCCGAATCCATA+TGG 0.475225 4.1:+6573472 None:intergenic
GGTAGTGGACAATCTTGAAA+AGG 0.476116 4.1:-6575616 MS.gene79454:CDS
AATATTGAAGAAGTTCCAAC+TGG 0.478486 4.1:-6575304 MS.gene79454:CDS
GACCCCTTCTTGCTCTGACA+TGG 0.481977 4.1:+6575275 None:intergenic
CTTGGTTAGACCAGATCATC+AGG 0.485638 4.1:-6574446 MS.gene79454:intron
CAAAAGAAGAGTAGTGGTGA+GGG 0.489373 4.1:-6575376 MS.gene79454:CDS
AATCTTCTTTGACTTTGCAA+TGG 0.497293 4.1:-6574482 MS.gene79454:CDS
ATCAAATTTACCTTAGACAA+GGG 0.503038 4.1:+6575516 None:intergenic
AGCAAAAGGTGTGGCATGGT+TGG 0.509890 4.1:+6574256 None:intergenic
TGCTGAATCATCCATGGTAG+TGG 0.516620 4.1:-6575631 None:intergenic
TGTCAGAGCAAGAAGGGGTC+AGG 0.517190 4.1:-6575272 MS.gene79454:CDS
ATATTGCAGCAACTTGTGCC+TGG 0.524669 4.1:-6574940 MS.gene79454:CDS
GTGGTAATTGTGTCAGCAAA+AGG 0.534827 4.1:+6574242 None:intergenic
ATAGCCACAGCCTTGCTGAA+AGG 0.535109 4.1:-6575243 MS.gene79454:CDS
ATACCCTTTCAGCAAGGCTG+TGG 0.536175 4.1:+6575239 None:intergenic
GAGGTCAAATACCTTCTTTG+AGG 0.536244 4.1:-6574153 MS.gene79454:intron
GATCTAAGGTACCTCAAAGA+AGG 0.549335 4.1:+6574142 None:intergenic
TGTAGGATGTCTCAAAAGAA+GGG 0.553029 4.1:-6575411 MS.gene79454:intron
TATCCATGTCAGAGCAAGAA+GGG 0.554936 4.1:-6575278 MS.gene79454:CDS
GTAGTGGACAATCTTGAAAA+GGG 0.561287 4.1:-6575615 MS.gene79454:CDS
CATAGTCATTATTAACAGGA+TGG 0.563260 4.1:+6574213 None:intergenic
TTCAAGATTGTCCACTACCA+TGG 0.563562 4.1:+6575620 None:intergenic
ACTTGTGCCTGGCTGTGATA+GGG 0.572729 4.1:-6574929 MS.gene79454:intron
AACTGGAAAGGCCCTTGTGT+TGG 0.580713 4.1:-6574675 MS.gene79454:CDS
CATCAAATTTACCTTAGACA+AGG 0.584025 4.1:+6575515 None:intergenic
TGATTATTTCATCCAACACA+AGG 0.586530 4.1:+6574663 None:intergenic
AAAGAAGAGTAGTGGTGAGG+GGG 0.586778 4.1:-6575374 MS.gene79454:CDS
AATGGACCTAGACACACTCT+TGG 0.596392 4.1:-6574464 MS.gene79454:CDS
GTCCTTACAAAATCAAGTAG+AGG 0.597808 4.1:-6574630 MS.gene79454:intron
AAAAGAAGAGTAGTGGTGAG+GGG 0.607886 4.1:-6575375 MS.gene79454:CDS
GTCTAACCAAGAGTGTGTCT+AGG 0.610495 4.1:+6574458 None:intergenic
ACAAAAGAAGAGTAGTGGTG+AGG 0.612710 4.1:-6575377 MS.gene79454:CDS
ATATCCATGTCAGAGCAAGA+AGG 0.615381 4.1:-6575279 MS.gene79454:CDS
TATTAAATAGGTAACTGGAA+AGG 0.623233 4.1:-6574687 MS.gene79454:intron
AGACTCACCCTATCACAGCC+AGG 0.626341 4.1:+6574922 None:intergenic
GATTATTTCATCCAACACAA+GGG 0.628005 4.1:+6574664 None:intergenic
TCAAATTTACCTTAGACAAG+GGG 0.638877 4.1:+6575517 None:intergenic
TATTGCATACCCTTTCAGCA+AGG 0.648025 4.1:+6575233 None:intergenic
CTGACATGGATATAACCAGT+TGG 0.650898 4.1:+6575289 None:intergenic
ATTAACAGGATGGAAGCTTG+TGG 0.660676 4.1:+6574223 None:intergenic
ATCCATGTCAGAGCAAGAAG+GGG 0.665171 4.1:-6575277 MS.gene79454:CDS
GAGCTGCACAATGGTACATG+TGG 0.691981 4.1:+6574278 None:intergenic
TGTCAGCAAAAGGTGTGGCA+TGG 0.694030 4.1:+6574252 None:intergenic
GCATGGTTGGAGCTGCACAA+TGG 0.724748 4.1:+6574269 None:intergenic
AATTGTGTCAGCAAAAGGTG+TGG 0.745401 4.1:+6574247 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATGTTGCAAAAAAAT+TGG - chr4.1:6574071-6574090 MS.gene79454:intron 15.0%
!! ATAGAAACATATTCAAAAAA+GGG + chr4.1:6573605-6573624 None:intergenic 15.0%
!! TAATTGTTGAAGTTATTAAA+GGG + chr4.1:6575089-6575108 None:intergenic 15.0%
!! TATAGAAACATATTCAAAAA+AGG + chr4.1:6573606-6573625 None:intergenic 15.0%
!! TGATACTATATGATTATAAT+TGG - chr4.1:6574964-6574983 MS.gene79454:CDS 15.0%
!! TTAATTGTTGAAGTTATTAA+AGG + chr4.1:6575090-6575109 None:intergenic 15.0%
!! TTCAATGAAAATTATTACAA+GGG + chr4.1:6574228-6574247 None:intergenic 15.0%
!!! AAGTACAACTTTTATATTTT+TGG + chr4.1:6574478-6574497 None:intergenic 15.0%
!! AAAACATAGTCATTATTAAC+AGG + chr4.1:6574868-6574887 None:intergenic 20.0%
!! ATTATTGCAAAATGTTAATC+AGG + chr4.1:6575431-6575450 None:intergenic 20.0%
!! CATTCCTTTAATATAGATAT+CGG - chr4.1:6574176-6574195 MS.gene79454:CDS 20.0%
!! CTTCAATGAAAATTATTACA+AGG + chr4.1:6574229-6574248 None:intergenic 20.0%
!! TAGGTTATTACAATTTACAA+CGG - chr4.1:6574005-6574024 MS.gene79454:intron 20.0%
!! TATCAAAATCTTATGATCTA+AGG + chr4.1:6574949-6574968 None:intergenic 20.0%
!! TTCTAAATATAAGTCCAATA+CGG - chr4.1:6575058-6575077 MS.gene79454:intron 20.0%
!! TTTCATATTAAATAGGTAAC+TGG - chr4.1:6574382-6574401 MS.gene79454:intron 20.0%
!!! ATTAACATTTTGCAATTTCA+AGG + chr4.1:6575199-6575218 None:intergenic 20.0%
!!! GATTTGGTTTCATATTAAAT+AGG - chr4.1:6574375-6574394 MS.gene79454:intron 20.0%
!!! TAACATTTTGCAATTTTAGA+GGG - chr4.1:6575378-6575397 MS.gene79454:CDS 20.0%
!!! TGTTAATAATGACTATGTTT+TGG - chr4.1:6574867-6574886 MS.gene79454:intron 20.0%
!!! TTAACATTTTGCAATTTTAG+AGG - chr4.1:6575377-6575396 MS.gene79454:CDS 20.0%
!!! TTATTGATTTTGTTACTTTG+TGG - chr4.1:6573636-6573655 MS.gene79454:intron 20.0%
!!! TTGAAATATCGGTTTTAAAT+AGG - chr4.1:6574322-6574341 MS.gene79454:intron 20.0%
!!! TTTTAAAATTACTACACTGA+TGG + chr4.1:6573926-6573945 None:intergenic 20.0%
!!! TTTTGGTAAAATGACTAAAT+TGG + chr4.1:6575469-6575488 None:intergenic 20.0%
! AAAAAACAAAAGAAGAGTAG+TGG - chr4.1:6573692-6573711 MS.gene79454:intron 25.0%
! AAACCGATATTTCAAATTGA+AGG + chr4.1:6574317-6574336 None:intergenic 25.0%
! AAGATGAATCTTGATATTCA+AGG + chr4.1:6575238-6575257 None:intergenic 25.0%
! ATATGAAACCAAATCAAATG+TGG + chr4.1:6574370-6574389 None:intergenic 25.0%
! ATCAAATTTACCTTAGACAA+GGG + chr4.1:6573561-6573580 None:intergenic 25.0%
! ATGTCCGATATCTATATTAA+AGG + chr4.1:6574183-6574202 None:intergenic 25.0%
! ATTACAATTTACAACGGAAA+GGG - chr4.1:6574011-6574030 MS.gene79454:intron 25.0%
! GTTTATAAAGTTAAGCTTGT+TGG + chr4.1:6574521-6574540 None:intergenic 25.0%
! TATTAAATAGGTAACTGGAA+AGG - chr4.1:6574387-6574406 MS.gene79454:intron 25.0%
! TATTACAATTTACAACGGAA+AGG - chr4.1:6574010-6574029 MS.gene79454:intron 25.0%
! TGTATGTATCAATGTTGTAT+CGG - chr4.1:6574256-6574275 MS.gene79454:CDS 25.0%
! TTGCAAAAAAATTGGATTTC+AGG - chr4.1:6574079-6574098 MS.gene79454:intron 25.0%
!! CAACTTCAATTTTTCTTCAA+GGG - chr4.1:6574896-6574915 MS.gene79454:intron 25.0%
!!! AAGAGATCGATTTTGATATT+TGG + chr4.1:6575131-6575150 None:intergenic 25.0%
!!! GATCAAAGTACTTTATGTTT+TGG + chr4.1:6575486-6575505 None:intergenic 25.0%
AATATTGAAGAAGTTCCAAC+TGG - chr4.1:6573770-6573789 MS.gene79454:intron 30.0%
ACAATGAGTTATGAACTTTG+AGG + chr4.1:6574050-6574069 None:intergenic 30.0%
ATCAACAAGTCTCTACATTA+TGG - chr4.1:6575158-6575177 MS.gene79454:intron 30.0%
ATGAATCTTGATATTCAAGG+AGG + chr4.1:6575235-6575254 None:intergenic 30.0%
ATTGCAAAGTCAAAGAAGAT+TGG + chr4.1:6574593-6574612 None:intergenic 30.0%
CAAAAGCTTCTTAATCCATA+TGG - chr4.1:6575587-6575606 MS.gene79454:CDS 30.0%
CATAGTCATTATTAACAGGA+TGG + chr4.1:6574864-6574883 None:intergenic 30.0%
CATCAAATTTACCTTAGACA+AGG + chr4.1:6573562-6573581 None:intergenic 30.0%
CCTTGAAGAAAAATTGAAGT+TGG + chr4.1:6574898-6574917 None:intergenic 30.0%
CTTCTTAATCCATATGGATT+CGG - chr4.1:6575593-6575612 MS.gene79454:CDS 30.0%
GAAGAAAAAAGAAGAGAAGA+AGG - chr4.1:6573736-6573755 MS.gene79454:intron 30.0%
GATTATTTCATCCAACACAA+GGG + chr4.1:6574413-6574432 None:intergenic 30.0%
TCAAATTTACCTTAGACAAG+GGG + chr4.1:6573560-6573579 None:intergenic 30.0%
TGATTATTTCATCCAACACA+AGG + chr4.1:6574414-6574433 None:intergenic 30.0%
! AATCTTCTTTGACTTTGCAA+TGG - chr4.1:6574592-6574611 MS.gene79454:intron 30.0%
! ACGCCTTCAATTTGAAATAT+CGG - chr4.1:6574311-6574330 MS.gene79454:CDS 30.0%
! CCAACTTCAATTTTTCTTCA+AGG - chr4.1:6574895-6574914 MS.gene79454:intron 30.0%
! TACCTCTACTTGATTTTGTA+AGG + chr4.1:6574449-6574468 None:intergenic 30.0%
! TATGAACTTTGAGGAAATTG+AGG + chr4.1:6574041-6574060 None:intergenic 30.0%
!! AATGTAGAGACTTGTTGATA+AGG + chr4.1:6575157-6575176 None:intergenic 30.0%
!! CGCTTGAATTTGTTATCAAA+TGG - chr4.1:6575260-6575279 MS.gene79454:CDS 30.0%
!! TGTTACTTTGTGGTATTTGT+AGG - chr4.1:6573646-6573665 MS.gene79454:intron 30.0%
!!! GCTAGCTTTTCATCATAATA+AGG + chr4.1:6575513-6575532 None:intergenic 30.0%
!!! GGTTTTAAATAGGTTTACGT+AGG - chr4.1:6574332-6574351 MS.gene79454:intron 30.0%
!!! TGTTGAGTTGCTATTTTTAG+TGG + chr4.1:6575319-6575338 None:intergenic 30.0%
ATTTGTCCTTGCGTTATTAG+AGG + chr4.1:6575353-6575372 None:intergenic 35.0%
CACTAACACCTATCTAGTAT+CGG - chr4.1:6574286-6574305 MS.gene79454:CDS 35.0%
GTCCTTACAAAATCAAGTAG+AGG - chr4.1:6574444-6574463 MS.gene79454:intron 35.0%
TGTAGGATGTCTCAAAAGAA+GGG - chr4.1:6573663-6573682 MS.gene79454:intron 35.0%
TTGTAGGATGTCTCAAAAGA+AGG - chr4.1:6573662-6573681 MS.gene79454:intron 35.0%
TTTCACAGCCACATTTGATT+TGG - chr4.1:6574359-6574378 MS.gene79454:intron 35.0%
TTTGTCCTTGCGTTATTAGA+GGG + chr4.1:6575352-6575371 None:intergenic 35.0%
! CTCTGTTACTGAATTTTCAG+AGG - chr4.1:6574740-6574759 MS.gene79454:intron 35.0%
! TATCATTTTCAGCACACTGA+TGG - chr4.1:6575536-6575555 MS.gene79454:CDS 35.0%
! TCAGAAAGCTGTTACTTCTA+TGG - chr4.1:6573494-6573513 MS.gene79454:CDS 35.0%
!! GTAGTGGACAATCTTGAAAA+GGG - chr4.1:6573459-6573478 MS.gene79454:CDS 35.0%
AAAAGAAGAGTAGTGGTGAG+GGG - chr4.1:6573699-6573718 MS.gene79454:intron 40.0%
AATTGTGTCAGCAAAAGGTG+TGG + chr4.1:6574830-6574849 None:intergenic 40.0%
ACAAAAGAAGAGTAGTGGTG+AGG - chr4.1:6573697-6573716 MS.gene79454:intron 40.0%
ATATCCATGTCAGAGCAAGA+AGG - chr4.1:6573795-6573814 MS.gene79454:intron 40.0%
ATTAACAGGATGGAAGCTTG+TGG + chr4.1:6574854-6574873 None:intergenic 40.0%
CAAAAGAAGAGTAGTGGTGA+GGG - chr4.1:6573698-6573717 MS.gene79454:intron 40.0%
CACAATGGTACATGTGGTAT+TGG + chr4.1:6574793-6574812 None:intergenic 40.0%
CTGACATGGATATAACCAGT+TGG + chr4.1:6573788-6573807 None:intergenic 40.0%
GAGGTCAAATACCTTCTTTG+AGG - chr4.1:6574921-6574940 MS.gene79454:intron 40.0%
GTGGTAATTGTGTCAGCAAA+AGG + chr4.1:6574835-6574854 None:intergenic 40.0%
TATCCATGTCAGAGCAAGAA+GGG - chr4.1:6573796-6573815 MS.gene79454:intron 40.0%
TATTGCATACCCTTTCAGCA+AGG + chr4.1:6573844-6573863 None:intergenic 40.0%
TATTTCCTCACCTGATGATC+TGG + chr4.1:6574641-6574660 None:intergenic 40.0%
! AATTGGCTCATGAGATGTCA+GGG - chr4.1:6574981-6575000 MS.gene79454:intron 40.0%
! ATCAATGTTGTATCGGTGTC+AGG - chr4.1:6574263-6574282 MS.gene79454:CDS 40.0%
! CAATTTTTCTTCAAGGGCAG+AGG - chr4.1:6574902-6574921 MS.gene79454:intron 40.0%
! GATCTAAGGTACCTCAAAGA+AGG + chr4.1:6574935-6574954 None:intergenic 40.0%
! GTGTTGTCAGACAAAAGTGT+CGG - chr4.1:6573975-6573994 MS.gene79454:intron 40.0%
! TAATTGGCTCATGAGATGTC+AGG - chr4.1:6574980-6574999 MS.gene79454:intron 40.0%
! TACTACACTGATGGAAGTGT+CGG + chr4.1:6573917-6573936 None:intergenic 40.0%
!! ACATGTGGTATTGGTGTTGA+TGG + chr4.1:6574784-6574803 None:intergenic 40.0%
!! ATTAAAGGGAAGCACCGTAT+TGG + chr4.1:6575075-6575094 None:intergenic 40.0%
!! GGTAGTGGACAATCTTGAAA+AGG - chr4.1:6573458-6573477 MS.gene79454:CDS 40.0%
AAAGAAGAGTAGTGGTGAGG+GGG - chr4.1:6573700-6573719 MS.gene79454:intron 45.0%
AATGGACCTAGACACACTCT+TGG - chr4.1:6574610-6574629 MS.gene79454:intron 45.0%
ACAGTCCCTCTAATAACGCA+AGG - chr4.1:6575344-6575363 MS.gene79454:CDS 45.0%
ATATTGCAGCAACTTGTGCC+TGG - chr4.1:6574134-6574153 MS.gene79454:intron 45.0%
ATCCATGTCAGAGCAAGAAG+GGG - chr4.1:6573797-6573816 MS.gene79454:intron 45.0%
CGGTGTTTGTGTTTGCGTTA+GGG + chr4.1:6573885-6573904 None:intergenic 45.0%
CTTGGTTAGACCAGATCATC+AGG - chr4.1:6574628-6574647 MS.gene79454:intron 45.0%
TGCTCTTCTACGTCCCAAAA+TGG + chr4.1:6575563-6575582 None:intergenic 45.0%
TGTTTGCGTTAGGGCTTCAT+AGG + chr4.1:6573876-6573895 None:intergenic 45.0%
TTAGACCAGATCATCAGGTG+AGG - chr4.1:6574633-6574652 MS.gene79454:CDS 45.0%
! ATTGGCTCATGAGATGTCAG+GGG - chr4.1:6574982-6575001 MS.gene79454:intron 45.0%
! GTCTAACCAAGAGTGTGTCT+AGG + chr4.1:6574619-6574638 None:intergenic 45.0%
! GTGTTGTTGCCGAATCCATA+TGG + chr4.1:6575605-6575624 None:intergenic 45.0%
! TCGGTGTTTGTGTTTGCGTT+AGG + chr4.1:6573886-6573905 None:intergenic 45.0%
!! AGCACACTGATGGACCATTT+TGG - chr4.1:6575546-6575565 MS.gene79454:CDS 45.0%
!! CAAAAGTGTCGGAGCTACAT+AGG - chr4.1:6573986-6574005 MS.gene79454:intron 45.0%
!! GCACACTGATGGACCATTTT+GGG - chr4.1:6575547-6575566 MS.gene79454:CDS 45.0%
!! TTTGAGTTGCCCCTTGTCTA+AGG - chr4.1:6573548-6573567 MS.gene79454:intron 45.0%
AACTGGAAAGGCCCTTGTGT+TGG - chr4.1:6574399-6574418 MS.gene79454:intron 50.0%
AACTTGTGCCTGGCTGTGAT+AGG - chr4.1:6574144-6574163 MS.gene79454:intron 50.0%
ACTTGTGCCTGGCTGTGATA+GGG - chr4.1:6574145-6574164 MS.gene79454:intron 50.0%
AGGCGTGACCGATACTAGAT+AGG + chr4.1:6574297-6574316 None:intergenic 50.0%
ATACCCTTTCAGCAAGGCTG+TGG + chr4.1:6573838-6573857 None:intergenic 50.0%
ATAGCCACAGCCTTGCTGAA+AGG - chr4.1:6573831-6573850 MS.gene79454:intron 50.0%
GAGCTGCACAATGGTACATG+TGG + chr4.1:6574799-6574818 None:intergenic 50.0%
GGACTATAACGCTGCTGAGT+TGG - chr4.1:6575399-6575418 MS.gene79454:CDS 50.0%
TAGCCACAGCCTTGCTGAAA+GGG - chr4.1:6573832-6573851 MS.gene79454:intron 50.0%
! AGCAAAAGGTGTGGCATGGT+TGG + chr4.1:6574821-6574840 None:intergenic 50.0%
! TGTCAGCAAAAGGTGTGGCA+TGG + chr4.1:6574825-6574844 None:intergenic 50.0%
!! GGAAGTGTCGGTGTTGTGTT+CGG + chr4.1:6573905-6573924 None:intergenic 50.0%
AGACTCACCCTATCACAGCC+AGG + chr4.1:6574155-6574174 None:intergenic 55.0%
AGTAGTGGTGAGGGGGTGAA+AGG - chr4.1:6573707-6573726 MS.gene79454:intron 55.0%
GACCCCTTCTTGCTCTGACA+TGG + chr4.1:6573802-6573821 None:intergenic 55.0%
GCATGGTTGGAGCTGCACAA+TGG + chr4.1:6574808-6574827 None:intergenic 55.0%
TGTCAGAGCAAGAAGGGGTC+AGG - chr4.1:6573802-6573821 MS.gene79454:intron 55.0%
TGGTGAGGGGGTGAAAGGAG+AGG - chr4.1:6573712-6573731 MS.gene79454:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 6573456 6575640 6573456 ID=MS.gene79454
chr4.1 mRNA 6573456 6575640 6573456 ID=MS.gene79454.t1;Parent=MS.gene79454
chr4.1 exon 6575527 6575640 6575527 ID=MS.gene79454.t1.exon1;Parent=MS.gene79454.t1
chr4.1 CDS 6575527 6575640 6575527 ID=cds.MS.gene79454.t1;Parent=MS.gene79454.t1
chr4.1 exon 6575243 6575428 6575243 ID=MS.gene79454.t1.exon2;Parent=MS.gene79454.t1
chr4.1 CDS 6575243 6575428 6575243 ID=cds.MS.gene79454.t1;Parent=MS.gene79454.t1
chr4.1 exon 6574930 6574995 6574930 ID=MS.gene79454.t1.exon3;Parent=MS.gene79454.t1
chr4.1 CDS 6574930 6574995 6574930 ID=cds.MS.gene79454.t1;Parent=MS.gene79454.t1
chr4.1 exon 6574631 6574699 6574631 ID=MS.gene79454.t1.exon4;Parent=MS.gene79454.t1
chr4.1 CDS 6574631 6574699 6574631 ID=cds.MS.gene79454.t1;Parent=MS.gene79454.t1
chr4.1 exon 6574447 6574536 6574447 ID=MS.gene79454.t1.exon5;Parent=MS.gene79454.t1
chr4.1 CDS 6574447 6574536 6574447 ID=cds.MS.gene79454.t1;Parent=MS.gene79454.t1
chr4.1 exon 6574154 6574336 6574154 ID=MS.gene79454.t1.exon6;Parent=MS.gene79454.t1
chr4.1 CDS 6574154 6574336 6574154 ID=cds.MS.gene79454.t1;Parent=MS.gene79454.t1
chr4.1 exon 6573456 6573548 6573456 ID=MS.gene79454.t1.exon7;Parent=MS.gene79454.t1
chr4.1 CDS 6573456 6573548 6573456 ID=cds.MS.gene79454.t1;Parent=MS.gene79454.t1
Gene Sequence

>MS.gene79454

ATGGTAGTGGACAATCTTGAAAAGGGTGAACAAGTTACTCAGAAAGCTGTTACTTCTATGGAGAAGAAAAGAAGAATCAGAAATAAGACTTCTTTGAGTTGCCCCTTGTCTAAGGTAAATTTGATGCAGTTTTTTTTTTTTTTTTACCCTTTTTTGAATATGTTTCTATATTGTTTCACGTTATTGATTTTGTTACTTTGTGGTATTTGTAGGATGTCTCAAAAGAAGGGAGAAGCAAAAAACAAAAGAAGAGTAGTGGTGAGGGGGTGAAAGGAGAGGAGAAGAAAAAAGAAGAGAAGAAGGATCAAAAGAAAAATATTGAAGAAGTTCCAACTGGTTATATCCATGTCAGAGCAAGAAGGGGTCAGGCAACAGATAGCCACAGCCTTGCTGAAAGGGTATGCAATACAGTTTTTGCCTATGAAGCCCTAACGCAAACACAAACACCGAACACAACACCGACACTTCCATCAGTGTAGTAATTTTAAAAAATTGAATGTAATAACATGTGTCAGTAGTGTGTTGTCAGACAAAAGTGTCGGAGCTACATAGGTTATTACAATTTACAACGGAAAGGGAATTCCTCAATTTCCTCAAAGTTCATAACTCATTGTTAATAAATGTTGCAAAAAAATTGGATTTCAGGTGAGAAGAGAAAAAATAAGTGAAAGAATGAAGATATTGCAGCAACTTGTGCCTGGCTGTGATAGGGTGAGTCTTCATTCCTTTAATATAGATATCGGACATAACATTGATACTGACACATTGACCCTTGTAATAATTTTCATTGAAGAAGTTATTGTATGTATCAATGTTGTATCGGTGTCAGGCACTAACACCTATCTAGTATCGGTCACGCCTTCAATTTGAAATATCGGTTTTAAATAGGTTTACGTAGGCTCTTTTCACAGCCACATTTGATTTGGTTTCATATTAAATAGGTAACTGGAAAGGCCCTTGTGTTGGATGAAATAATCAATTATGTACAGTCCTTACAAAATCAAGTAGAGGTAATGCTGCCAAAAATATAAAAGTTGTACTTTTGAATCATTGATTCATAAACCAACAAGCTTAACTTTATAAACTTCTATATTTCTGATGCAGTTCTTGTCAATGAAACTTGCTTCAGTGAATCCAATCTTCTTTGACTTTGCAATGGACCTAGACACACTCTTGGTTAGACCAGATCATCAGGTGAGGAAATATAACTCAGTAATTAATTTTCATGTACTTGAATTTATATTAGCTGACTCAAACAAATTTATTAATGATGAAATAATGAAACTCTGTTACTGAATTTTCAGAGGTTAAATAGCATAACATCACCATCAACACCAATACCACATGTACCATTGTGCAGCTCCAACCATGCCACACCTTTTGCTGACACAATTACCACAAGCTTCCATCCTGTTAATAATGACTATGTTTTGGATTATCCAACTTCAATTTTTCTTCAAGGGCAGAGGTCAAATACCTTCTTTGAGGTACCTTAGATCATAAGATTTTGATACTATATGATTATAATTGGCTCATGAGATGTCAGGGGATACAATTATGTAATTAGCATACTAGTAAGTTTGAAAATTTTTGTCATTATTTTTCTAAATATAAGTCCAATACGGTGCTTCCCTTTAATAACTTCAACAATTAAATATATGTATACAAGTGACCAAATATCAAAATCGATCTCTTGCACCTTATCAACAAGTCTCTACATTATGGATATTTTGAAATGATCCTTGAAATTGCAAAATGTTAATCAAGTCTATAACACCTCCTTGAATATCAAGATTCATCTTTACGCTTGAATTTGTTATCAAATGGATCTCTGACATTATTCACTTAGTTCAAAATGAACCACTAAAAATAGCAACTCAACATCGTTACAGTCCCTCTAATAACGCAAGGACAAATCTGATTAACATTTTGCAATTTTAGAGGGACTATAACGCTGCTGAGTTGGTACAAACCTGATTAACATTTTGCAATAATTATTAAGTAAAATATCCAATTTAGTCATTTTACCAAAACATAAAGTACTTTGATCATTTCCTTATTATGATGAAAAGCTAGCATATATCATTTTCAGCACACTGATGGACCATTTTGGGACGTAGAAGAGCAACGACAAAAGCTTCTTAATCCATATGGATTCGGCAACAACACATGTTCCTTAAATTAA
Protein sequence

>MS.gene79454.t1

MVVDNLEKGEQVTQKAVTSMEKKRRIRNKTSLSCPLSKDVSKEGRSKKQKKSSGEGVKGEEKKKEEKKDQKKNIEEVPTGYIHVRARRGQATDSHSLAERVRREKISERMKILQQLVPGCDRVTGKALVLDEIINYVQSLQNQVEFLSMKLASVNPIFFDFAMDLDTLLVRPDHQRLNSITSPSTPIPHVPLCSSNHATPFADTITTSFHPVNNDYVLDYPTSIFLQGQRSNTFFEHTDGPFWDVEEQRQKLLNPYGFGNNTCSLN