Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79957.t1 | XP_024636235.1 | 94.2 | 243 | 14 | 0 | 1 | 243 | 1 | 243 | 4.30E-110 | 407.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79957.t1 | Q9M202 | 27.6 | 217 | 104 | 5 | 9 | 196 | 6 | 198 | 1.6e-09 | 64.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79957.t1 | A0A396I2K7 | 67.3 | 245 | 74 | 1 | 1 | 239 | 1 | 245 | 1.2e-66 | 262.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene79957.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79957.t1 | MTR_4g036615 | 80.769 | 104 | 20 | 0 | 78 | 181 | 1 | 104 | 1.96e-56 | 176 |
| MS.gene79957.t1 | MTR_3g408380 | 27.547 | 265 | 148 | 7 | 3 | 229 | 2 | 260 | 8.69e-20 | 85.9 |
| MS.gene79957.t1 | MTR_4g100860 | 27.820 | 266 | 144 | 8 | 9 | 229 | 6 | 268 | 1.15e-17 | 80.9 |
| MS.gene79957.t1 | MTR_3g408420 | 25.806 | 279 | 139 | 7 | 7 | 232 | 6 | 269 | 1.82e-16 | 77.0 |
| MS.gene79957.t1 | MTR_3g008310 | 26.038 | 265 | 158 | 7 | 3 | 229 | 2 | 266 | 7.38e-16 | 75.1 |
| MS.gene79957.t1 | MTR_0160s0030 | 41.975 | 81 | 37 | 1 | 170 | 240 | 239 | 319 | 4.46e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79957.t1 | AT3G60580 | 30.566 | 265 | 116 | 6 | 9 | 229 | 6 | 246 | 4.93e-19 | 84.3 |
| MS.gene79957.t1 | AT1G02030 | 45.570 | 79 | 30 | 2 | 171 | 236 | 160 | 238 | 3.02e-13 | 67.8 |
| MS.gene79957.t1 | AT5G61470 | 41.096 | 73 | 31 | 2 | 168 | 229 | 225 | 296 | 4.15e-12 | 65.1 |
Find 60 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAACACTTTGTTGCAATT+TGG | 0.166386 | 4.3:-63415832 | None:intergenic |
| TTCTGTGTTTGTTGTATTTC+GGG | 0.187551 | 4.3:-63415403 | None:intergenic |
| ATTCTGTGTTTGTTGTATTT+CGG | 0.237824 | 4.3:-63415404 | None:intergenic |
| TACTCAAGCTGTAGACAATT+TGG | 0.312612 | 4.3:+63415330 | MS.gene79957:CDS |
| AATTGGATCTCTTGCGGGTT+GGG | 0.313326 | 4.3:-63415507 | None:intergenic |
| TGAACACTTTGTTGCAATTT+GGG | 0.315013 | 4.3:-63415831 | None:intergenic |
| TGAGTATTAGTTTCAAGCTT+TGG | 0.337317 | 4.3:-63415313 | None:intergenic |
| GCCGCAATTGGATCTCTTGC+GGG | 0.342035 | 4.3:-63415512 | None:intergenic |
| CAATTGGATCTCTTGCGGGT+TGG | 0.402481 | 4.3:-63415508 | None:intergenic |
| ATTAAGTTCGCGGCCGCAAT+TGG | 0.437633 | 4.3:-63415524 | None:intergenic |
| ATTTGTGGGAAGTGGTTCTC+CGG | 0.453707 | 4.3:+63415235 | MS.gene79957:CDS |
| GTTTCAAGCTTTGGAGCAAT+CGG | 0.454553 | 4.3:-63415304 | None:intergenic |
| TTCCGATCTTATCAGGCTCT+TGG | 0.457389 | 4.3:+63415742 | MS.gene79957:CDS |
| TACAGAATCACAAATCAATA+CGG | 0.462697 | 4.3:-63415928 | None:intergenic |
| TTCTCCGGTGGGAAAGCCCT+TGG | 0.463478 | 4.3:+63415250 | MS.gene79957:CDS |
| TTGCAATTTGGGCAGTGAAA+AGG | 0.463868 | 4.3:-63415820 | None:intergenic |
| GGAAAGCCTGTGGGATTTGT+GGG | 0.467351 | 4.3:+63415221 | MS.gene79957:CDS |
| GGGAAAGCCTGTGGGATTTG+TGG | 0.468727 | 4.3:+63415220 | MS.gene79957:CDS |
| CGATCTTATCAGGCTCTTGG+TGG | 0.468794 | 4.3:+63415745 | MS.gene79957:CDS |
| GCCTCCAAGGGCTTTCCCAC+CGG | 0.472807 | 4.3:-63415254 | None:intergenic |
| AGATCTCATGTGGCCTCCAA+GGG | 0.485485 | 4.3:-63415266 | None:intergenic |
| TCCGGTGGGAAAGCCCTTGG+AGG | 0.494800 | 4.3:+63415253 | MS.gene79957:CDS |
| TTCAAGTCTGCTCAGGCACT+CGG | 0.500253 | 4.3:+63415850 | MS.gene79957:CDS |
| CCCCATAACATCAAAACACC+TGG | 0.500612 | 4.3:-63415580 | None:intergenic |
| GGCCGCAATTGGATCTCTTG+CGG | 0.503301 | 4.3:-63415513 | None:intergenic |
| TCTGTTTGAGTTTGCAGAGG+AGG | 0.504442 | 4.3:-63415455 | None:intergenic |
| AACGATGGCGAAAGCAGTAC+AGG | 0.507873 | 4.3:+63415658 | MS.gene79957:CDS |
| GCACCTCTCACAGTTGAACC+TGG | 0.509618 | 4.3:-63415710 | None:intergenic |
| CAGCCGCAGCATTAAGTTCG+CGG | 0.512384 | 4.3:-63415534 | None:intergenic |
| TTCTCTGTTTGAGTTTGCAG+AGG | 0.512537 | 4.3:-63415458 | None:intergenic |
| GAGATCTCATGTGGCCTCCA+AGG | 0.514030 | 4.3:-63415267 | None:intergenic |
| GAAGATATGTCAAGAGATCC+AGG | 0.516117 | 4.3:+63415562 | MS.gene79957:CDS |
| TGTGGGAAGTGGTTCTCCGG+TGG | 0.517822 | 4.3:+63415238 | MS.gene79957:CDS |
| ACTTTGAAATTCCTTAGCAC+TGG | 0.521740 | 4.3:-63415896 | None:intergenic |
| CAAAGTGTTCAAGTCTGCTC+AGG | 0.524975 | 4.3:+63415843 | MS.gene79957:CDS |
| CACAAGCAGACTCTGAAGCC+AGG | 0.539423 | 4.3:+63415692 | MS.gene79957:CDS |
| GTGTTTGTTGTATTTCGGGA+AGG | 0.552910 | 4.3:-63415399 | None:intergenic |
| CCTGTGGGATTTGTGGGAAG+TGG | 0.558125 | 4.3:+63415227 | MS.gene79957:CDS |
| GTGGGAAGTGGTTCTCCGGT+GGG | 0.561353 | 4.3:+63415239 | MS.gene79957:CDS |
| GATCTTATCAGGCTCTTGGT+GGG | 0.571526 | 4.3:+63415746 | MS.gene79957:CDS |
| GGTACACTCTTCCAGTGCTA+AGG | 0.575791 | 4.3:+63415885 | MS.gene79957:CDS |
| GCAACTATGGAGAAGAGCGA+TGG | 0.579019 | 4.3:+63415199 | None:intergenic |
| CACCAAGAGCCTGATAAGAT+CGG | 0.582662 | 4.3:-63415744 | None:intergenic |
| AAGAGCGATGGGAAAGCCTG+TGG | 0.598960 | 4.3:+63415211 | MS.gene79957:CDS |
| AGGCACTCGGTGGACACAAG+AGG | 0.606213 | 4.3:+63415863 | MS.gene79957:CDS |
| ACAGAATCACAAATCAATAC+GGG | 0.613334 | 4.3:-63415927 | None:intergenic |
| CAACTATGGAGAAGAGCGAT+GGG | 0.616240 | 4.3:+63415200 | None:intergenic |
| TACTGCAAAAGAGACAATGA+TGG | 0.621835 | 4.3:+63415778 | MS.gene79957:CDS |
| AAGCCAGGTTCAACTGTGAG+AGG | 0.622627 | 4.3:+63415707 | MS.gene79957:CDS |
| ATCACAAATCAATACGGGTG+TGG | 0.631203 | 4.3:-63415922 | None:intergenic |
| GGCACTCGGTGGACACAAGA+GGG | 0.632635 | 4.3:+63415864 | MS.gene79957:CDS |
| CCACTTCCCACAAATCCCAC+AGG | 0.647858 | 4.3:-63415227 | None:intergenic |
| AGAGCGATGGGAAAGCCTGT+GGG | 0.654994 | 4.3:+63415212 | MS.gene79957:CDS |
| CGGCCGCGAACTTAATGCTG+CGG | 0.660556 | 4.3:+63415531 | MS.gene79957:CDS |
| AGGTTCAACTGTGAGAGGTG+CGG | 0.663022 | 4.3:+63415712 | MS.gene79957:CDS |
| TTCGGGAAGGCAAGTCTGTG+AGG | 0.669270 | 4.3:-63415386 | None:intergenic |
| AAGTCTGCTCAGGCACTCGG+TGG | 0.670340 | 4.3:+63415853 | MS.gene79957:CDS |
| AGGCCACATGAGATCTCACA+TGG | 0.682156 | 4.3:+63415273 | MS.gene79957:CDS |
| ACCCGCAAGAGATCCAATTG+CGG | 0.686880 | 4.3:+63415511 | MS.gene79957:CDS |
| GAACCATGTGAGATCTCATG+TGG | 0.730676 | 4.3:-63415276 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATCAAGAAACAAAAAATGA+GGG | + | chr4.3:63415633-63415652 | MS.gene79957:CDS | 25.0% |
| !!! | ATTCTGTGTTTGTTGTATTT+CGG | - | chr4.3:63415407-63415426 | None:intergenic | 25.0% |
| AAAAATGTTCCGATCTTATC+AGG | + | chr4.3:63415735-63415754 | MS.gene79957:CDS | 30.0% | |
| GGATCAAGAAACAAAAAATG+AGG | + | chr4.3:63415632-63415651 | MS.gene79957:CDS | 30.0% | |
| TGAACACTTTGTTGCAATTT+GGG | - | chr4.3:63415834-63415853 | None:intergenic | 30.0% | |
| TTACAAAGATGCGCAAAAAA+GGG | + | chr4.3:63415606-63415625 | MS.gene79957:CDS | 30.0% | |
| TTGAACACTTTGTTGCAATT+TGG | - | chr4.3:63415835-63415854 | None:intergenic | 30.0% | |
| TTTACAAAGATGCGCAAAAA+AGG | + | chr4.3:63415605-63415624 | MS.gene79957:CDS | 30.0% | |
| ! | TGAGTATTAGTTTCAAGCTT+TGG | - | chr4.3:63415316-63415335 | None:intergenic | 30.0% |
| !! | AAGCAAATTGATTTTGACGA+TGG | - | chr4.3:63415366-63415385 | None:intergenic | 30.0% |
| !! | AGCAAATTGATTTTGACGAT+GGG | - | chr4.3:63415365-63415384 | None:intergenic | 30.0% |
| !! | TTCTGTGTTTGTTGTATTTC+GGG | - | chr4.3:63415406-63415425 | None:intergenic | 30.0% |
| CAAAAAATGAGGGAAAACGA+TGG | + | chr4.3:63415643-63415662 | MS.gene79957:CDS | 35.0% | |
| TACTCAAGCTGTAGACAATT+TGG | + | chr4.3:63415330-63415349 | MS.gene79957:CDS | 35.0% | |
| TACTGCAAAAGAGACAATGA+TGG | + | chr4.3:63415778-63415797 | MS.gene79957:CDS | 35.0% | |
| !! | ACTTTGAAATTCCTTAGCAC+TGG | - | chr4.3:63415899-63415918 | None:intergenic | 35.0% |
| !!! | ATCCAGGTGTTTTGATGTTA+TGG | + | chr4.3:63415578-63415597 | MS.gene79957:CDS | 35.0% |
| !!! | ATTTTGACGATGGGTTGAAT+CGG | - | chr4.3:63415356-63415375 | None:intergenic | 35.0% |
| !!! | CGAAGTTTTGTTTCATGGAT+AGG | - | chr4.3:63415432-63415451 | None:intergenic | 35.0% |
| !!! | TCCAGGTGTTTTGATGTTAT+GGG | + | chr4.3:63415579-63415598 | MS.gene79957:CDS | 35.0% |
| AAAGATGCGCAAAAAAGGGA+AGG | + | chr4.3:63415610-63415629 | MS.gene79957:CDS | 40.0% | |
| AAGATGCGCAAAAAAGGGAA+GGG | + | chr4.3:63415611-63415630 | MS.gene79957:CDS | 40.0% | |
| GAAGATATGTCAAGAGATCC+AGG | + | chr4.3:63415562-63415581 | MS.gene79957:CDS | 40.0% | |
| TTGCAATTTGGGCAGTGAAA+AGG | - | chr4.3:63415823-63415842 | None:intergenic | 40.0% | |
| ! | TTCTCTGTTTGAGTTTGCAG+AGG | - | chr4.3:63415461-63415480 | None:intergenic | 40.0% |
| !! | GTGTTTGTTGTATTTCGGGA+AGG | - | chr4.3:63415402-63415421 | None:intergenic | 40.0% |
| !! | GTTTCAAGCTTTGGAGCAAT+CGG | - | chr4.3:63415307-63415326 | None:intergenic | 40.0% |
| !!! | CCAGGTGTTTTGATGTTATG+GGG | + | chr4.3:63415580-63415599 | MS.gene79957:CDS | 40.0% |
| AATTGGATCTCTTGCGGGTT+GGG | - | chr4.3:63415510-63415529 | None:intergenic | 45.0% | |
| ATTTGTGGGAAGTGGTTCTC+CGG | + | chr4.3:63415235-63415254 | MS.gene79957:CDS | 45.0% | |
| CACCAAGAGCCTGATAAGAT+CGG | - | chr4.3:63415747-63415766 | None:intergenic | 45.0% | |
| CCCCATAACATCAAAACACC+TGG | - | chr4.3:63415583-63415602 | None:intergenic | 45.0% | |
| GAACCATGTGAGATCTCATG+TGG | - | chr4.3:63415279-63415298 | None:intergenic | 45.0% | |
| GATCTTATCAGGCTCTTGGT+GGG | + | chr4.3:63415746-63415765 | MS.gene79957:CDS | 45.0% | |
| TTCCGATCTTATCAGGCTCT+TGG | + | chr4.3:63415742-63415761 | MS.gene79957:CDS | 45.0% | |
| ! | CAAAGTGTTCAAGTCTGCTC+AGG | + | chr4.3:63415843-63415862 | MS.gene79957:CDS | 45.0% |
| ! | TCTGTTTGAGTTTGCAGAGG+AGG | - | chr4.3:63415458-63415477 | None:intergenic | 45.0% |
| !!! | GGCAGCGAAGTTTTGTTTCA+TGG | - | chr4.3:63415437-63415456 | None:intergenic | 45.0% |
| AACGATGGCGAAAGCAGTAC+AGG | + | chr4.3:63415658-63415677 | MS.gene79957:CDS | 50.0% | |
| AAGCCAGGTTCAACTGTGAG+AGG | + | chr4.3:63415707-63415726 | MS.gene79957:CDS | 50.0% | |
| ACCCGCAAGAGATCCAATTG+CGG | + | chr4.3:63415511-63415530 | MS.gene79957:CDS | 50.0% | |
| AGATCTCATGTGGCCTCCAA+GGG | - | chr4.3:63415269-63415288 | None:intergenic | 50.0% | |
| AGGCCACATGAGATCTCACA+TGG | + | chr4.3:63415273-63415292 | MS.gene79957:CDS | 50.0% | |
| AGGTTCAACTGTGAGAGGTG+CGG | + | chr4.3:63415712-63415731 | MS.gene79957:CDS | 50.0% | |
| ATTAAGTTCGCGGCCGCAAT+TGG | - | chr4.3:63415527-63415546 | None:intergenic | 50.0% | |
| CAATTGGATCTCTTGCGGGT+TGG | - | chr4.3:63415511-63415530 | None:intergenic | 50.0% | |
| CGATCTTATCAGGCTCTTGG+TGG | + | chr4.3:63415745-63415764 | MS.gene79957:CDS | 50.0% | |
| GGAAAGCCTGTGGGATTTGT+GGG | + | chr4.3:63415221-63415240 | MS.gene79957:CDS | 50.0% | |
| GGTACACTCTTCCAGTGCTA+AGG | + | chr4.3:63415885-63415904 | MS.gene79957:CDS | 50.0% | |
| TTCAAGTCTGCTCAGGCACT+CGG | + | chr4.3:63415850-63415869 | MS.gene79957:CDS | 50.0% | |
| !!! | TCTTGCGGGTTGGGTTTTTC+AGG | - | chr4.3:63415501-63415520 | None:intergenic | 50.0% |
| AAGAGCGATGGGAAAGCCTG+TGG | + | chr4.3:63415211-63415230 | MS.gene79957:CDS | 55.0% | |
| AGAGCGATGGGAAAGCCTGT+GGG | + | chr4.3:63415212-63415231 | MS.gene79957:CDS | 55.0% | |
| CAGCCGCAGCATTAAGTTCG+CGG | - | chr4.3:63415537-63415556 | None:intergenic | 55.0% | |
| CCACTTCCCACAAATCCCAC+AGG | - | chr4.3:63415230-63415249 | None:intergenic | 55.0% | |
| CCTGTGGGATTTGTGGGAAG+TGG | + | chr4.3:63415227-63415246 | MS.gene79957:CDS | 55.0% | |
| GAGATCTCATGTGGCCTCCA+AGG | - | chr4.3:63415270-63415289 | None:intergenic | 55.0% | |
| GCACCTCTCACAGTTGAACC+TGG | - | chr4.3:63415713-63415732 | None:intergenic | 55.0% | |
| GCCGCAATTGGATCTCTTGC+GGG | - | chr4.3:63415515-63415534 | None:intergenic | 55.0% | |
| GGCCGCAATTGGATCTCTTG+CGG | - | chr4.3:63415516-63415535 | None:intergenic | 55.0% | |
| GGGAAAGCCTGTGGGATTTG+TGG | + | chr4.3:63415220-63415239 | MS.gene79957:CDS | 55.0% | |
| TTCGGGAAGGCAAGTCTGTG+AGG | - | chr4.3:63415389-63415408 | None:intergenic | 55.0% | |
| !! | CACAAGCAGACTCTGAAGCC+AGG | + | chr4.3:63415692-63415711 | MS.gene79957:CDS | 55.0% |
| !!! | GCGGGTTGGGTTTTTCAGGT+TGG | - | chr4.3:63415497-63415516 | None:intergenic | 55.0% |
| AAGTCTGCTCAGGCACTCGG+TGG | + | chr4.3:63415853-63415872 | MS.gene79957:CDS | 60.0% | |
| CGGCCGCGAACTTAATGCTG+CGG | + | chr4.3:63415531-63415550 | MS.gene79957:CDS | 60.0% | |
| GTGGGAAGTGGTTCTCCGGT+GGG | + | chr4.3:63415239-63415258 | MS.gene79957:CDS | 60.0% | |
| TGTGGGAAGTGGTTCTCCGG+TGG | + | chr4.3:63415238-63415257 | MS.gene79957:CDS | 60.0% | |
| TTCTCCGGTGGGAAAGCCCT+TGG | + | chr4.3:63415250-63415269 | MS.gene79957:CDS | 60.0% | |
| ! | AGGCACTCGGTGGACACAAG+AGG | + | chr4.3:63415863-63415882 | MS.gene79957:CDS | 60.0% |
| ! | GGCACTCGGTGGACACAAGA+GGG | + | chr4.3:63415864-63415883 | MS.gene79957:CDS | 60.0% |
| GCCTCCAAGGGCTTTCCCAC+CGG | - | chr4.3:63415257-63415276 | None:intergenic | 65.0% | |
| TCCGGTGGGAAAGCCCTTGG+AGG | + | chr4.3:63415253-63415272 | MS.gene79957:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 63415205 | 63415936 | 63415205 | ID=MS.gene79957 |
| chr4.3 | mRNA | 63415205 | 63415936 | 63415205 | ID=MS.gene79957.t1;Parent=MS.gene79957 |
| chr4.3 | exon | 63415205 | 63415936 | 63415205 | ID=MS.gene79957.t1.exon1;Parent=MS.gene79957.t1 |
| chr4.3 | CDS | 63415205 | 63415936 | 63415205 | ID=cds.MS.gene79957.t1;Parent=MS.gene79957.t1 |
| Gene Sequence |
| Protein sequence |
