Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80106.t1 | ABD33026.2 | 78.2 | 206 | 44 | 1 | 10 | 215 | 361 | 565 | 1.80E-88 | 335.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80106.t1 | Q6NQJ7 | 31.7 | 205 | 131 | 4 | 8 | 210 | 4 | 201 | 5.7e-22 | 105.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80106.t1 | Q2HSZ7 | 78.2 | 206 | 44 | 1 | 10 | 215 | 361 | 565 | 1.3e-88 | 335.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene80106.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80106.t1 | MTR_7g446060 | 73.118 | 93 | 24 | 1 | 77 | 169 | 1 | 92 | 5.07e-43 | 142 |
| MS.gene80106.t1 | MTR_7g031620 | 62.353 | 85 | 28 | 1 | 3 | 87 | 2 | 82 | 1.29e-30 | 108 |
| MS.gene80106.t1 | MTR_1g008290 | 34.091 | 132 | 83 | 3 | 63 | 194 | 17 | 144 | 2.30e-19 | 81.3 |
| MS.gene80106.t1 | MTR_0856s0010 | 36.441 | 118 | 73 | 2 | 11 | 127 | 44 | 160 | 1.22e-18 | 79.7 |
| MS.gene80106.t1 | MTR_6g051425 | 29.054 | 148 | 105 | 0 | 68 | 215 | 92 | 239 | 1.03e-17 | 80.5 |
| MS.gene80106.t1 | MTR_0558s0020 | 24.255 | 235 | 145 | 6 | 11 | 217 | 46 | 275 | 4.99e-16 | 75.5 |
| MS.gene80106.t1 | MTR_1g083640 | 23.894 | 226 | 139 | 6 | 12 | 209 | 62 | 282 | 1.88e-14 | 70.9 |
| MS.gene80106.t1 | MTR_8g446020 | 22.500 | 160 | 119 | 2 | 61 | 217 | 78 | 235 | 2.60e-11 | 61.6 |
| MS.gene80106.t1 | MTR_1g013060 | 26.768 | 198 | 131 | 4 | 11 | 197 | 34 | 228 | 4.37e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80106.t1 | AT1G76320 | 31.707 | 205 | 131 | 4 | 8 | 210 | 4 | 201 | 9.25e-26 | 104 |
| MS.gene80106.t1 | AT1G76320 | 31.707 | 205 | 131 | 4 | 8 | 210 | 4 | 201 | 9.25e-26 | 104 |
| MS.gene80106.t1 | AT1G76320 | 31.707 | 205 | 131 | 4 | 8 | 210 | 4 | 201 | 9.42e-26 | 104 |
| MS.gene80106.t1 | AT1G76320 | 31.707 | 205 | 131 | 4 | 8 | 210 | 4 | 201 | 1.25e-25 | 104 |
| MS.gene80106.t1 | AT4G15090 | 28.293 | 205 | 141 | 4 | 16 | 215 | 66 | 269 | 7.90e-23 | 96.7 |
| MS.gene80106.t1 | AT4G15090 | 28.293 | 205 | 141 | 4 | 16 | 215 | 66 | 269 | 7.90e-23 | 96.7 |
| MS.gene80106.t1 | AT4G15090 | 28.293 | 205 | 141 | 4 | 16 | 215 | 66 | 269 | 7.90e-23 | 96.7 |
| MS.gene80106.t1 | AT4G15090 | 28.293 | 205 | 141 | 4 | 16 | 215 | 66 | 269 | 7.90e-23 | 96.7 |
| MS.gene80106.t1 | AT4G15090 | 28.293 | 205 | 141 | 4 | 16 | 215 | 66 | 269 | 7.90e-23 | 96.7 |
| MS.gene80106.t1 | AT3G22170 | 24.775 | 222 | 127 | 5 | 12 | 216 | 81 | 279 | 4.30e-16 | 77.0 |
| MS.gene80106.t1 | AT3G22170 | 24.775 | 222 | 127 | 5 | 12 | 216 | 81 | 279 | 4.30e-16 | 77.0 |
| MS.gene80106.t1 | AT4G38180 | 23.223 | 211 | 154 | 3 | 8 | 211 | 80 | 289 | 8.92e-14 | 70.1 |
| MS.gene80106.t1 | AT1G52520 | 31.500 | 200 | 117 | 7 | 16 | 209 | 100 | 285 | 1.32e-11 | 63.9 |
| MS.gene80106.t1 | AT1G80010 | 26.168 | 214 | 136 | 7 | 16 | 215 | 110 | 315 | 1.63e-11 | 63.5 |
Find 25 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCTAGATGTATAGGTTT+TGG | 0.261658 | 8.4:+69068666 | MS.gene80106:CDS |
| AAGAATTATGCTAGATGTAT+AGG | 0.283443 | 8.4:+69068660 | MS.gene80106:CDS |
| TGGAACAATTACTATTTCTA+AGG | 0.295449 | 8.4:+69068854 | MS.gene80106:CDS |
| GCGAGATGTAGGGCTGCTTA+TGG | 0.310927 | 8.4:+69069233 | MS.gene80106:CDS |
| ATATAGACCCTAGTATAAAA+AGG | 0.351811 | 8.4:+69068943 | MS.gene80106:CDS |
| TTAAAATATCTTGATTTGCT+TGG | 0.366169 | 8.4:-69068909 | None:intergenic |
| GGATATGATAACCTTACATT+CGG | 0.409109 | 8.4:+69069038 | MS.gene80106:CDS |
| ACTATTTCTAAGGTTGCTCT+TGG | 0.411911 | 8.4:+69068864 | MS.gene80106:CDS |
| AAGTGAGGCGGCTTCGACTT+GGG | 0.428800 | 8.4:+69069090 | MS.gene80106:CDS |
| CGAGATGTAGGGCTGCTTAT+GGG | 0.435390 | 8.4:+69069234 | MS.gene80106:CDS |
| TACTTACATTAATCCAAGCT+TGG | 0.439441 | 8.4:-69068983 | None:intergenic |
| TAGCATGAAAACACATTGTA+AGG | 0.444794 | 8.4:+69068803 | MS.gene80106:CDS |
| TGGAGGTTAATGACCAAGCT+TGG | 0.446409 | 8.4:+69068970 | MS.gene80106:CDS |
| CTGCTTATGGGATGAGTTAG+TGG | 0.448679 | 8.4:+69069246 | MS.gene80106:CDS |
| TTTGGGCAGATGCGAGATGT+AGG | 0.485083 | 8.4:+69069222 | MS.gene80106:CDS |
| TTGGGCAGATGCGAGATGTA+GGG | 0.512318 | 8.4:+69069223 | MS.gene80106:CDS |
| AAAGTGAGGCGGCTTCGACT+TGG | 0.512577 | 8.4:+69069089 | MS.gene80106:CDS |
| TACTCACATAGCTTCTTGCA+CGG | 0.535718 | 8.4:-69068752 | None:intergenic |
| AGGCGGCTTCGACTTGGGAT+AGG | 0.542513 | 8.4:+69069095 | MS.gene80106:CDS |
| GATATGATAACCTTACATTC+GGG | 0.543620 | 8.4:+69069039 | MS.gene80106:CDS |
| AAGCTTGGATTAATGTAAGT+AGG | 0.576078 | 8.4:+69068985 | MS.gene80106:CDS |
| AATGTATGTGTGTCTTCAGA+TGG | 0.584390 | 8.4:+69068834 | MS.gene80106:CDS |
| GAAACTACATAGACAAAGTG+AGG | 0.617900 | 8.4:+69069075 | MS.gene80106:CDS |
| TCTATGGTTGTTGAAGCAAG+TGG | 0.653447 | 8.4:+69069017 | MS.gene80106:CDS |
| ACTACATAGACAAAGTGAGG+CGG | 0.739143 | 8.4:+69069078 | MS.gene80106:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTAACAAAATAATTTTGAA+TGG | - | chr8.4:69069132-69069151 | None:intergenic | 15.0% |
| !!! | ATTTGGCTTTAAAAGATTTT+TGG | - | chr8.4:69068781-69068800 | None:intergenic | 20.0% |
| !!! | GAAAAAAGTATTTTACTCTA+GGG | + | chr8.4:69068724-69068743 | MS.gene80106:CDS | 20.0% |
| !!! | TAGTGACTTTTATTATGTAA+TGG | + | chr8.4:69069166-69069185 | MS.gene80106:CDS | 20.0% |
| !!! | TTAAAATATCTTGATTTGCT+TGG | - | chr8.4:69068912-69068931 | None:intergenic | 20.0% |
| !!! | TTAATGTAAGTAGGAATTTT+TGG | + | chr8.4:69068994-69069013 | MS.gene80106:CDS | 20.0% |
| ! | AAGAATTATGCTAGATGTAT+AGG | + | chr8.4:69068660-69068679 | MS.gene80106:CDS | 25.0% |
| ! | AGCAAAATCAGTACAAAAAA+TGG | + | chr8.4:69068693-69068712 | MS.gene80106:CDS | 25.0% |
| ! | ATATAGACCCTAGTATAAAA+AGG | + | chr8.4:69068943-69068962 | MS.gene80106:CDS | 25.0% |
| !!! | CTTTTATTATGTAATGGATG+TGG | + | chr8.4:69069172-69069191 | MS.gene80106:CDS | 25.0% |
| !!! | GGAAAAAAGTATTTTACTCT+AGG | + | chr8.4:69068723-69068742 | MS.gene80106:CDS | 25.0% |
| !!! | TCGTTTACGAAATGTTTTTT+GGG | + | chr8.4:69069205-69069224 | MS.gene80106:CDS | 25.0% |
| !!! | TGGAACAATTACTATTTCTA+AGG | + | chr8.4:69068854-69068873 | MS.gene80106:CDS | 25.0% |
| AAGCTTGGATTAATGTAAGT+AGG | + | chr8.4:69068985-69069004 | MS.gene80106:CDS | 30.0% | |
| AGTACAAAAAATGGAGATGA+TGG | + | chr8.4:69068702-69068721 | MS.gene80106:CDS | 30.0% | |
| GATATGATAACCTTACATTC+GGG | + | chr8.4:69069039-69069058 | MS.gene80106:CDS | 30.0% | |
| GGATATGATAACCTTACATT+CGG | + | chr8.4:69069038-69069057 | MS.gene80106:CDS | 30.0% | |
| TACTTACATTAATCCAAGCT+TGG | - | chr8.4:69068986-69069005 | None:intergenic | 30.0% | |
| TAGCATGAAAACACATTGTA+AGG | + | chr8.4:69068803-69068822 | MS.gene80106:CDS | 30.0% | |
| TAGTATAAAAAGGAGATTGG+AGG | + | chr8.4:69068953-69068972 | MS.gene80106:CDS | 30.0% | |
| ! | CCAATCTCCTTTTTATACTA+GGG | - | chr8.4:69068953-69068972 | None:intergenic | 30.0% |
| ! | TATGCTAGATGTATAGGTTT+TGG | + | chr8.4:69068666-69068685 | MS.gene80106:CDS | 30.0% |
| ! | TCCAATCTCCTTTTTATACT+AGG | - | chr8.4:69068954-69068973 | None:intergenic | 30.0% |
| ! | TTACAATGTGTTTTCATGCT+AGG | - | chr8.4:69068804-69068823 | None:intergenic | 30.0% |
| !!! | AAGTAGGAATTTTTGGTCTA+TGG | + | chr8.4:69069001-69069020 | MS.gene80106:CDS | 30.0% |
| !!! | GTCGTTTACGAAATGTTTTT+TGG | + | chr8.4:69069204-69069223 | MS.gene80106:CDS | 30.0% |
| AATGTATGTGTGTCTTCAGA+TGG | + | chr8.4:69068834-69068853 | MS.gene80106:CDS | 35.0% | |
| CCCTAGTATAAAAAGGAGAT+TGG | + | chr8.4:69068950-69068969 | MS.gene80106:CDS | 35.0% | |
| GAAACTACATAGACAAAGTG+AGG | + | chr8.4:69069075-69069094 | MS.gene80106:CDS | 35.0% | |
| ! | TGTGTTTTCATGCTAGGATT+TGG | - | chr8.4:69068798-69068817 | None:intergenic | 35.0% |
| !! | ACTATTTCTAAGGTTGCTCT+TGG | + | chr8.4:69068864-69068883 | MS.gene80106:CDS | 35.0% |
| !!! | CATCTTTTTTCCCGAATGTA+AGG | - | chr8.4:69069052-69069071 | None:intergenic | 35.0% |
| ACTACATAGACAAAGTGAGG+CGG | + | chr8.4:69069078-69069097 | MS.gene80106:CDS | 40.0% | |
| TACTCACATAGCTTCTTGCA+CGG | - | chr8.4:69068755-69068774 | None:intergenic | 40.0% | |
| TCTATGGTTGTTGAAGCAAG+TGG | + | chr8.4:69069017-69069036 | MS.gene80106:CDS | 40.0% | |
| TGGAGGTTAATGACCAAGCT+TGG | + | chr8.4:69068970-69068989 | MS.gene80106:CDS | 45.0% | |
| CGAGATGTAGGGCTGCTTAT+GGG | + | chr8.4:69069234-69069253 | MS.gene80106:CDS | 50.0% | |
| TTGGGCAGATGCGAGATGTA+GGG | + | chr8.4:69069223-69069242 | MS.gene80106:CDS | 50.0% | |
| TTTGGGCAGATGCGAGATGT+AGG | + | chr8.4:69069222-69069241 | MS.gene80106:CDS | 50.0% | |
| AAAGTGAGGCGGCTTCGACT+TGG | + | chr8.4:69069089-69069108 | MS.gene80106:CDS | 55.0% | |
| AAGTGAGGCGGCTTCGACTT+GGG | + | chr8.4:69069090-69069109 | MS.gene80106:CDS | 55.0% | |
| GCGAGATGTAGGGCTGCTTA+TGG | + | chr8.4:69069233-69069252 | MS.gene80106:CDS | 55.0% | |
| !! | AGGCGGCTTCGACTTGGGAT+AGG | + | chr8.4:69069095-69069114 | MS.gene80106:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 69068609 | 69069265 | 69068609 | ID=MS.gene80106 |
| chr8.4 | mRNA | 69068609 | 69069265 | 69068609 | ID=MS.gene80106.t1;Parent=MS.gene80106 |
| chr8.4 | exon | 69068609 | 69069265 | 69068609 | ID=MS.gene80106.t1.exon1;Parent=MS.gene80106.t1 |
| chr8.4 | CDS | 69068609 | 69069265 | 69068609 | ID=cds.MS.gene80106.t1;Parent=MS.gene80106.t1 |
| Gene Sequence |
| Protein sequence |
