Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80166.t1 | XP_003603265.1 | 94.1 | 290 | 17 | 0 | 1 | 290 | 1 | 290 | 6.00E-111 | 410.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80166.t1 | Q940T9 | 50.5 | 374 | 87 | 12 | 1 | 289 | 1 | 361 | 2.1e-40 | 167.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80166.t1 | G7JPB9 | 94.1 | 290 | 17 | 0 | 1 | 290 | 1 | 290 | 4.3e-111 | 410.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene80166.t1 | TF | C2C2-CO-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80166.t1 | MTR_3g105710 | 97.931 | 290 | 6 | 0 | 1 | 290 | 1 | 290 | 0.0 | 592 |
| MS.gene80166.t1 | MTR_1g013450 | 54.717 | 318 | 114 | 7 | 1 | 290 | 1 | 316 | 1.21e-106 | 313 |
| MS.gene80166.t1 | MTR_4g128930 | 51.506 | 332 | 100 | 11 | 4 | 280 | 20 | 345 | 1.05e-89 | 271 |
| MS.gene80166.t1 | MTR_7g018170 | 63.043 | 92 | 34 | 0 | 3 | 94 | 23 | 114 | 1.95e-37 | 137 |
| MS.gene80166.t1 | MTR_7g018170 | 71.429 | 70 | 9 | 2 | 231 | 289 | 326 | 395 | 6.38e-23 | 97.8 |
| MS.gene80166.t1 | MTR_5g021580 | 50.602 | 83 | 33 | 1 | 5 | 79 | 4 | 86 | 1.23e-20 | 87.4 |
| MS.gene80166.t1 | MTR_5g021580 | 50.602 | 83 | 33 | 1 | 5 | 79 | 4 | 86 | 1.29e-20 | 87.4 |
| MS.gene80166.t1 | MTR_5g069480 | 43.333 | 90 | 51 | 0 | 5 | 94 | 4 | 93 | 2.55e-19 | 86.3 |
| MS.gene80166.t1 | MTR_5g069480 | 43.333 | 90 | 51 | 0 | 5 | 94 | 4 | 93 | 3.06e-19 | 87.4 |
| MS.gene80166.t1 | MTR_1g023260 | 43.299 | 97 | 45 | 1 | 6 | 92 | 5 | 101 | 6.65e-19 | 84.7 |
| MS.gene80166.t1 | MTR_1g023260 | 43.299 | 97 | 45 | 1 | 6 | 92 | 5 | 101 | 7.07e-19 | 82.8 |
| MS.gene80166.t1 | MTR_2g073370 | 44.792 | 96 | 44 | 1 | 6 | 92 | 5 | 100 | 1.41e-18 | 84.0 |
| MS.gene80166.t1 | MTR_4g067320 | 43.750 | 96 | 45 | 1 | 6 | 92 | 5 | 100 | 1.45e-18 | 83.2 |
| MS.gene80166.t1 | MTR_4g067320 | 43.750 | 96 | 45 | 1 | 6 | 92 | 5 | 100 | 1.47e-18 | 83.2 |
| MS.gene80166.t1 | MTR_2g099010 | 42.708 | 96 | 46 | 1 | 6 | 92 | 5 | 100 | 2.09e-17 | 80.1 |
| MS.gene80166.t1 | MTR_2g099010 | 42.708 | 96 | 46 | 1 | 6 | 92 | 5 | 100 | 2.10e-17 | 80.1 |
| MS.gene80166.t1 | MTR_4g071200 | 39.175 | 97 | 49 | 1 | 6 | 92 | 5 | 101 | 6.89e-17 | 78.2 |
| MS.gene80166.t1 | MTR_3g117320 | 38.144 | 97 | 50 | 1 | 6 | 92 | 5 | 101 | 7.86e-17 | 78.2 |
| MS.gene80166.t1 | MTR_7g108150 | 39.326 | 89 | 53 | 1 | 6 | 94 | 20 | 107 | 4.21e-16 | 77.8 |
| MS.gene80166.t1 | MTR_3g082630 | 39.216 | 102 | 61 | 1 | 5 | 106 | 4 | 104 | 4.58e-16 | 78.2 |
| MS.gene80166.t1 | MTR_3g082630 | 44.872 | 78 | 40 | 1 | 202 | 279 | 327 | 401 | 4.23e-11 | 63.5 |
| MS.gene80166.t1 | MTR_3g034680 | 61.702 | 47 | 18 | 0 | 51 | 97 | 12 | 58 | 1.32e-15 | 71.2 |
| MS.gene80166.t1 | MTR_2g088900 | 45.122 | 82 | 45 | 0 | 5 | 86 | 100 | 181 | 8.11e-15 | 74.7 |
| MS.gene80166.t1 | MTR_2g088900 | 38.318 | 107 | 46 | 2 | 189 | 279 | 411 | 513 | 5.43e-12 | 66.2 |
| MS.gene80166.t1 | MTR_3g113070 | 38.298 | 94 | 47 | 1 | 6 | 88 | 5 | 98 | 1.23e-14 | 72.0 |
| MS.gene80166.t1 | MTR_7g083540 | 44.444 | 72 | 40 | 0 | 6 | 77 | 5 | 76 | 1.79e-13 | 70.5 |
| MS.gene80166.t1 | MTR_1g109350 | 39.189 | 74 | 45 | 0 | 4 | 77 | 3 | 76 | 1.89e-12 | 67.0 |
| MS.gene80166.t1 | MTR_1g110870 | 61.818 | 55 | 17 | 1 | 224 | 274 | 370 | 424 | 3.94e-12 | 66.6 |
| MS.gene80166.t1 | MTR_8g104190 | 70.455 | 44 | 13 | 0 | 232 | 275 | 358 | 401 | 5.83e-12 | 65.9 |
| MS.gene80166.t1 | MTR_3g092780 | 49.231 | 65 | 32 | 1 | 219 | 283 | 576 | 639 | 8.51e-12 | 65.9 |
| MS.gene80166.t1 | MTR_3g092780 | 49.231 | 65 | 32 | 1 | 219 | 283 | 577 | 640 | 8.76e-12 | 65.9 |
| MS.gene80166.t1 | MTR_5g072780 | 66.667 | 45 | 15 | 0 | 230 | 274 | 172 | 216 | 9.03e-12 | 63.5 |
| MS.gene80166.t1 | MTR_3g092780 | 49.231 | 65 | 32 | 1 | 219 | 283 | 617 | 680 | 9.13e-12 | 65.9 |
| MS.gene80166.t1 | MTR_3g092780 | 49.231 | 65 | 32 | 1 | 219 | 283 | 618 | 681 | 9.22e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80166.t1 | AT5G24930 | 52.267 | 375 | 79 | 13 | 1 | 289 | 1 | 361 | 1.72e-95 | 286 |
| MS.gene80166.t1 | AT2G24790 | 53.767 | 292 | 98 | 10 | 2 | 277 | 4 | 274 | 1.72e-93 | 278 |
| MS.gene80166.t1 | AT5G57660 | 42.775 | 346 | 131 | 10 | 2 | 289 | 18 | 354 | 5.49e-74 | 231 |
| MS.gene80166.t1 | AT3G02380 | 40.058 | 342 | 142 | 9 | 3 | 289 | 13 | 346 | 2.77e-72 | 226 |
| MS.gene80166.t1 | AT5G15850 | 41.499 | 347 | 142 | 8 | 3 | 289 | 9 | 354 | 4.31e-70 | 221 |
| MS.gene80166.t1 | AT5G15840 | 39.058 | 361 | 135 | 14 | 6 | 289 | 20 | 372 | 6.68e-65 | 208 |
| MS.gene80166.t1 | AT2G24790 | 55.367 | 177 | 58 | 5 | 2 | 171 | 4 | 166 | 3.52e-57 | 183 |
| MS.gene80166.t1 | AT5G15840 | 41.398 | 186 | 83 | 7 | 6 | 166 | 20 | 204 | 9.79e-37 | 132 |
| MS.gene80166.t1 | AT1G49130 | 28.435 | 313 | 176 | 10 | 4 | 280 | 19 | 319 | 6.17e-23 | 96.7 |
| MS.gene80166.t1 | AT1G49130 | 28.435 | 313 | 176 | 10 | 4 | 280 | 26 | 326 | 6.54e-23 | 96.7 |
| MS.gene80166.t1 | AT4G38960 | 35.570 | 149 | 74 | 3 | 5 | 138 | 4 | 145 | 3.41e-20 | 86.3 |
| MS.gene80166.t1 | AT4G38960 | 35.570 | 149 | 74 | 3 | 5 | 138 | 4 | 145 | 3.41e-20 | 86.3 |
| MS.gene80166.t1 | AT2G21320 | 49.398 | 83 | 34 | 1 | 5 | 79 | 4 | 86 | 5.86e-20 | 85.1 |
| MS.gene80166.t1 | AT1G78600 | 44.792 | 96 | 44 | 1 | 6 | 92 | 5 | 100 | 4.65e-19 | 85.5 |
| MS.gene80166.t1 | AT1G78600 | 42.017 | 119 | 56 | 3 | 6 | 111 | 5 | 123 | 5.02e-19 | 85.9 |
| MS.gene80166.t1 | AT2G47890 | 42.857 | 91 | 50 | 1 | 4 | 94 | 11 | 99 | 4.33e-18 | 82.8 |
| MS.gene80166.t1 | AT2G47890 | 42.857 | 91 | 50 | 1 | 4 | 94 | 11 | 99 | 4.85e-18 | 83.2 |
| MS.gene80166.t1 | AT2G47890 | 27.273 | 308 | 161 | 14 | 3 | 276 | 53 | 331 | 8.44e-14 | 70.9 |
| MS.gene80166.t1 | AT2G47890 | 42.857 | 91 | 50 | 1 | 4 | 94 | 11 | 99 | 6.27e-18 | 81.3 |
| MS.gene80166.t1 | AT1G06040 | 43.750 | 96 | 45 | 1 | 6 | 92 | 5 | 100 | 6.88e-18 | 81.6 |
| MS.gene80166.t1 | AT1G06040 | 43.750 | 96 | 45 | 1 | 6 | 92 | 5 | 100 | 1.04e-17 | 79.3 |
| MS.gene80166.t1 | AT1G75540 | 40.404 | 99 | 47 | 1 | 6 | 92 | 5 | 103 | 1.21e-17 | 82.0 |
| MS.gene80166.t1 | AT2G33500 | 42.697 | 89 | 51 | 0 | 6 | 94 | 12 | 100 | 3.02e-17 | 81.6 |
| MS.gene80166.t1 | AT2G33500 | 52.632 | 57 | 27 | 0 | 220 | 276 | 345 | 401 | 1.03e-12 | 68.2 |
| MS.gene80166.t1 | AT2G33500 | 42.697 | 89 | 51 | 0 | 6 | 94 | 12 | 100 | 3.03e-17 | 81.6 |
| MS.gene80166.t1 | AT2G33500 | 52.632 | 57 | 27 | 0 | 220 | 276 | 344 | 400 | 1.08e-12 | 68.2 |
| MS.gene80166.t1 | AT2G31380 | 34.177 | 158 | 87 | 4 | 6 | 153 | 5 | 155 | 1.30e-16 | 77.8 |
| MS.gene80166.t1 | AT1G28050 | 41.573 | 89 | 52 | 0 | 6 | 94 | 9 | 97 | 4.26e-16 | 78.2 |
| MS.gene80166.t1 | AT1G28050 | 56.000 | 50 | 22 | 0 | 228 | 277 | 381 | 430 | 8.87e-11 | 62.4 |
| MS.gene80166.t1 | AT5G48250 | 38.043 | 92 | 55 | 1 | 5 | 94 | 4 | 95 | 1.10e-14 | 73.9 |
| MS.gene80166.t1 | AT3G07650 | 45.205 | 73 | 40 | 0 | 5 | 77 | 4 | 76 | 3.10e-14 | 72.4 |
| MS.gene80166.t1 | AT3G07650 | 58.333 | 48 | 20 | 0 | 232 | 279 | 315 | 362 | 9.29e-11 | 62.4 |
| MS.gene80166.t1 | AT3G07650 | 45.205 | 73 | 40 | 0 | 5 | 77 | 4 | 76 | 3.10e-14 | 72.4 |
| MS.gene80166.t1 | AT3G07650 | 58.333 | 48 | 20 | 0 | 232 | 279 | 315 | 362 | 9.29e-11 | 62.4 |
| MS.gene80166.t1 | AT3G07650 | 45.205 | 73 | 40 | 0 | 5 | 77 | 4 | 76 | 3.10e-14 | 72.4 |
| MS.gene80166.t1 | AT3G07650 | 58.333 | 48 | 20 | 0 | 232 | 279 | 315 | 362 | 9.29e-11 | 62.4 |
| MS.gene80166.t1 | AT3G07650 | 45.205 | 73 | 40 | 0 | 5 | 77 | 4 | 76 | 3.10e-14 | 72.4 |
| MS.gene80166.t1 | AT3G07650 | 58.333 | 48 | 20 | 0 | 232 | 279 | 315 | 362 | 9.29e-11 | 62.4 |
| MS.gene80166.t1 | AT4G10240 | 33.714 | 175 | 81 | 7 | 6 | 165 | 5 | 159 | 4.28e-14 | 68.9 |
| MS.gene80166.t1 | AT4G39070 | 37.755 | 98 | 51 | 1 | 5 | 92 | 4 | 101 | 1.72e-13 | 68.9 |
| MS.gene80166.t1 | AT1G73870 | 55.556 | 63 | 27 | 1 | 213 | 275 | 327 | 388 | 2.39e-13 | 70.1 |
| MS.gene80166.t1 | AT4G15250 | 40.476 | 84 | 50 | 0 | 6 | 89 | 5 | 88 | 4.64e-13 | 68.9 |
| MS.gene80166.t1 | AT1G68190 | 38.824 | 85 | 50 | 1 | 4 | 88 | 41 | 123 | 6.51e-13 | 68.9 |
| MS.gene80166.t1 | AT1G68190 | 38.824 | 85 | 50 | 1 | 4 | 88 | 12 | 94 | 7.76e-13 | 68.6 |
| MS.gene80166.t1 | AT1G68190 | 38.824 | 85 | 50 | 1 | 4 | 88 | 12 | 94 | 7.76e-13 | 68.6 |
| MS.gene80166.t1 | AT1G25440 | 36.029 | 136 | 72 | 5 | 158 | 286 | 288 | 415 | 4.86e-12 | 66.2 |
| MS.gene80166.t1 | AT3G21880 | 38.202 | 89 | 53 | 1 | 6 | 94 | 5 | 91 | 1.39e-11 | 64.7 |
| MS.gene80166.t1 | AT3G21880 | 38.202 | 89 | 53 | 1 | 6 | 94 | 9 | 95 | 1.40e-11 | 64.7 |
| MS.gene80166.t1 | AT3G21880 | 38.202 | 89 | 53 | 1 | 6 | 94 | 9 | 95 | 1.40e-11 | 64.7 |
| MS.gene80166.t1 | AT5G14370 | 63.043 | 46 | 17 | 0 | 232 | 277 | 294 | 339 | 1.80e-11 | 64.3 |
| MS.gene80166.t1 | AT4G25990 | 60.870 | 46 | 18 | 0 | 232 | 277 | 341 | 386 | 2.34e-11 | 64.3 |
| MS.gene80166.t1 | AT1G68520 | 68.182 | 44 | 14 | 0 | 232 | 275 | 357 | 400 | 2.43e-11 | 64.3 |
Find 74 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGCAGTTGACTCCATTTC+TGG | 0.143468 | 3.4:+92337320 | MS.gene80166:CDS |
| GAAACTAGGCCTAGGATTAA+AGG | 0.280004 | 3.4:+92337278 | MS.gene80166:CDS |
| CCGCAGAGGAAGGCGGAGTC+AGG | 0.296878 | 3.4:-92336446 | None:intergenic |
| TTCCGAAGCTATTCCGTTCA+TGG | 0.298068 | 3.4:+92336817 | MS.gene80166:CDS |
| AGGCTAGGGTTATGAGGTAT+AGG | 0.358381 | 3.4:+92337192 | MS.gene80166:CDS |
| GACGGTGTTGTTCCCGTTCA+TGG | 0.369225 | 3.4:+92336872 | MS.gene80166:CDS |
| GCAAACCTCCCTTTAATCCT+AGG | 0.392261 | 3.4:-92337287 | None:intergenic |
| CCGTTCATGGATCTCGATTA+CGG | 0.395344 | 3.4:+92336830 | MS.gene80166:CDS |
| TTTGTTAGCGGCGTGGACTT+TGG | 0.416071 | 3.4:-92336480 | None:intergenic |
| GGATCGAAGTTGCCATGAAC+GGG | 0.430806 | 3.4:-92336884 | None:intergenic |
| TGGCGGCGGATCGTGAGGCT+AGG | 0.437396 | 3.4:+92337177 | MS.gene80166:CDS |
| GATTCGAAGAACGGAGTAAC+GGG | 0.439450 | 3.4:-92336656 | None:intergenic |
| AGATATCGAACTGTGGTTAC+GGG | 0.444139 | 3.4:+92337150 | MS.gene80166:CDS |
| GAGATATCGAACTGTGGTTA+CGG | 0.445942 | 3.4:+92337149 | MS.gene80166:CDS |
| AACGATGCCGTATCCAGAAA+TGG | 0.452434 | 3.4:-92337333 | None:intergenic |
| GATGAGTTGAGATCTGCTTT+CGG | 0.461772 | 3.4:-92336776 | None:intergenic |
| GAGAGTGACGCGAGGGTGAC+GGG | 0.479132 | 3.4:-92336510 | None:intergenic |
| TCCGACACGGTGTTCCCGTC+AGG | 0.479338 | 3.4:-92337128 | None:intergenic |
| TTTAACAGAGTGAGAAGTGT+TGG | 0.480554 | 3.4:-92336678 | None:intergenic |
| GGCTAGGGTTATGAGGTATA+GGG | 0.482522 | 3.4:+92337193 | MS.gene80166:CDS |
| TGGTTACGGGACGGTGGCGG+CGG | 0.488159 | 3.4:+92337163 | MS.gene80166:CDS |
| AAACTAGGCCTAGGATTAAA+GGG | 0.490422 | 3.4:+92337279 | MS.gene80166:CDS |
| AGTGTGGATGTCACGGTCAC+AGG | 0.492186 | 3.4:-92336600 | None:intergenic |
| ATCCATGAACGGAATAGCTT+CGG | 0.492715 | 3.4:-92336819 | None:intergenic |
| TTATGAGGTATAGGGAGAAG+AGG | 0.496368 | 3.4:+92337201 | MS.gene80166:CDS |
| TTCTTGTAAGCTGAAACAAA+TGG | 0.496560 | 3.4:-92336905 | None:intergenic |
| GTTGACTCCATTTCTGGATA+CGG | 0.500668 | 3.4:+92337326 | MS.gene80166:CDS |
| GGCGGCGGATCGTGAGGCTA+GGG | 0.502731 | 3.4:+92337178 | MS.gene80166:CDS |
| TTAGGTATCGTCTTCGTCGA+TGG | 0.503016 | 3.4:+92337091 | MS.gene80166:intron |
| GTAAAGCATATGCGGAAACT+AGG | 0.510714 | 3.4:+92337264 | MS.gene80166:CDS |
| CTGTGGTTACGGGACGGTGG+CGG | 0.513006 | 3.4:+92337160 | MS.gene80166:CDS |
| CGCAGAGGAAGGCGGAGTCA+GGG | 0.518288 | 3.4:-92336445 | None:intergenic |
| TCGTCTTCGTCGATGGAGGT+TGG | 0.523017 | 3.4:+92337098 | MS.gene80166:CDS |
| AGGTTGGAGTTGTTCCTGAC+GGG | 0.524585 | 3.4:+92337114 | MS.gene80166:CDS |
| ATGAAGCTGAAGCTGCTTCG+TGG | 0.533741 | 3.4:+92336741 | MS.gene80166:CDS |
| GGATTCGAAGAACGGAGTAA+CGG | 0.536432 | 3.4:-92336657 | None:intergenic |
| GCTTTACACGTGACATGTGC+AGG | 0.538227 | 3.4:-92336554 | None:intergenic |
| AGAGAGTGACGCGAGGGTGA+CGG | 0.539503 | 3.4:-92336511 | None:intergenic |
| GGCGTGGACTTTGGAGTCAC+AGG | 0.543388 | 3.4:-92336471 | None:intergenic |
| GTCACAGGCGCCGCAGAGGA+AGG | 0.545693 | 3.4:-92336456 | None:intergenic |
| TCGGAATCAGAGAAAAGATA+AGG | 0.547155 | 3.4:-92336800 | None:intergenic |
| GACGGTGGCGGCGGATCGTG+AGG | 0.549105 | 3.4:+92337172 | MS.gene80166:CDS |
| TGGATCGAAGTTGCCATGAA+CGG | 0.558189 | 3.4:-92336885 | None:intergenic |
| CGCTCGTGACGTGCGGCGAG+AGG | 0.558417 | 3.4:-92336629 | None:intergenic |
| GCAGTAGAGAGTAGCGGTGG+CGG | 0.563031 | 3.4:-92336420 | None:intergenic |
| GAAGTGTTGGATTCGAAGAA+CGG | 0.565969 | 3.4:-92336665 | None:intergenic |
| GATGCAGAGAGAAGCTGCAT+CGG | 0.576026 | 3.4:-92336576 | None:intergenic |
| CATATGCGGAAACTAGGCCT+AGG | 0.577094 | 3.4:+92337270 | MS.gene80166:CDS |
| ACGGGAGGCGAGTTTGTTAG+CGG | 0.583367 | 3.4:-92336492 | None:intergenic |
| GGTATCGTCTTCGTCGATGG+AGG | 0.585063 | 3.4:+92337094 | MS.gene80166:intron |
| ACAGGCGCCGCAGAGGAAGG+CGG | 0.586506 | 3.4:-92336453 | None:intergenic |
| GATTAGCAGTGTGGATGTCA+CGG | 0.589251 | 3.4:-92336607 | None:intergenic |
| CGCTTCTCGTAAAGCATATG+CGG | 0.592961 | 3.4:+92337256 | MS.gene80166:CDS |
| GAGGTTGGAGTTGTTCCTGA+CGG | 0.607148 | 3.4:+92337113 | MS.gene80166:CDS |
| GGGAACACGCTCGTGACGTG+CGG | 0.610507 | 3.4:-92336636 | None:intergenic |
| ATCGTGAGGCTAGGGTTATG+AGG | 0.613658 | 3.4:+92337186 | MS.gene80166:CDS |
| TGGAGTCACAGGCGCCGCAG+AGG | 0.616423 | 3.4:-92336460 | None:intergenic |
| AGGCGAGTTTGTTAGCGGCG+TGG | 0.630051 | 3.4:-92336487 | None:intergenic |
| TGACTAATCTGCGACTGTGA+CGG | 0.630286 | 3.4:-92336962 | None:intergenic |
| GCGGCAGTAGAGAGTAGCGG+TGG | 0.638763 | 3.4:-92336423 | None:intergenic |
| AGGGCGGCAGTAGAGAGTAG+CGG | 0.646899 | 3.4:-92336426 | None:intergenic |
| CGGCGAGAGGATTAGCAGTG+TGG | 0.659805 | 3.4:-92336616 | None:intergenic |
| AGAGGAAGGCGGAGTCAGGG+CGG | 0.663446 | 3.4:-92336442 | None:intergenic |
| AGTGACGCGAGGGTGACGGG+AGG | 0.667459 | 3.4:-92336507 | None:intergenic |
| CCTGACTCCGCCTTCCTCTG+CGG | 0.671751 | 3.4:+92336446 | MS.gene80166:CDS |
| ATCGAACTGTGGTTACGGGA+CGG | 0.672790 | 3.4:+92337154 | MS.gene80166:CDS |
| TCCTGACGGGAACACCGTGT+CGG | 0.682213 | 3.4:+92337127 | MS.gene80166:CDS |
| GAACTGTGGTTACGGGACGG+TGG | 0.687113 | 3.4:+92337157 | MS.gene80166:CDS |
| CTTCGCAGAGAGTGACGCGA+GGG | 0.694593 | 3.4:-92336517 | None:intergenic |
| CTAGGCCTAGGATTAAAGGG+AGG | 0.700310 | 3.4:+92337282 | MS.gene80166:CDS |
| ACTTCGCAGAGAGTGACGCG+AGG | 0.710854 | 3.4:-92336518 | None:intergenic |
| CCGTAATCGAGATCCATGAA+CGG | 0.730276 | 3.4:-92336830 | None:intergenic |
| ACAGTTCGATATCTCCGACA+CGG | 0.744977 | 3.4:-92337141 | None:intergenic |
| GTGTCGGAGATATCGAACTG+TGG | 0.760977 | 3.4:+92337143 | MS.gene80166:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTGTTGTGATTAATT+AGG | + | chr3.4:92337073-92337092 | MS.gene80166:intron | 15.0% |
| !!! | TAAGTTTGTTTTTATTGACA+TGG | + | chr3.4:92337014-92337033 | MS.gene80166:intron | 20.0% |
| ! | GTAATTGAACATAAAAACGA+CGG | + | chr3.4:92336854-92336873 | MS.gene80166:CDS | 25.0% |
| TTCTTGTAAGCTGAAACAAA+TGG | - | chr3.4:92336908-92336927 | None:intergenic | 30.0% | |
| AAACTAGGCCTAGGATTAAA+GGG | + | chr3.4:92337279-92337298 | MS.gene80166:CDS | 35.0% | |
| TCGGAATCAGAGAAAAGATA+AGG | - | chr3.4:92336803-92336822 | None:intergenic | 35.0% | |
| ! | TTTAACAGAGTGAGAAGTGT+TGG | - | chr3.4:92336681-92336700 | None:intergenic | 35.0% |
| AGATATCGAACTGTGGTTAC+GGG | + | chr3.4:92337150-92337169 | MS.gene80166:CDS | 40.0% | |
| ATCCATGAACGGAATAGCTT+CGG | - | chr3.4:92336822-92336841 | None:intergenic | 40.0% | |
| GAAACTAGGCCTAGGATTAA+AGG | + | chr3.4:92337278-92337297 | MS.gene80166:CDS | 40.0% | |
| GAGATATCGAACTGTGGTTA+CGG | + | chr3.4:92337149-92337168 | MS.gene80166:CDS | 40.0% | |
| GTAAAGCATATGCGGAAACT+AGG | + | chr3.4:92337264-92337283 | MS.gene80166:CDS | 40.0% | |
| TTATGAGGTATAGGGAGAAG+AGG | + | chr3.4:92337201-92337220 | MS.gene80166:CDS | 40.0% | |
| ! | GATGAGTTGAGATCTGCTTT+CGG | - | chr3.4:92336779-92336798 | None:intergenic | 40.0% |
| ! | GTTGACTCCATTTCTGGATA+CGG | + | chr3.4:92337326-92337345 | MS.gene80166:CDS | 40.0% |
| !! | GAAGTGTTGGATTCGAAGAA+CGG | - | chr3.4:92336668-92336687 | None:intergenic | 40.0% |
| AACGATGCCGTATCCAGAAA+TGG | - | chr3.4:92337336-92337355 | None:intergenic | 45.0% | |
| ACAGTTCGATATCTCCGACA+CGG | - | chr3.4:92337144-92337163 | None:intergenic | 45.0% | |
| AGGCTAGGGTTATGAGGTAT+AGG | + | chr3.4:92337192-92337211 | MS.gene80166:CDS | 45.0% | |
| CCGTAATCGAGATCCATGAA+CGG | - | chr3.4:92336833-92336852 | None:intergenic | 45.0% | |
| CCGTTCATGGATCTCGATTA+CGG | + | chr3.4:92336830-92336849 | MS.gene80166:CDS | 45.0% | |
| CGCTTCTCGTAAAGCATATG+CGG | + | chr3.4:92337256-92337275 | MS.gene80166:CDS | 45.0% | |
| GATTAGCAGTGTGGATGTCA+CGG | - | chr3.4:92336610-92336629 | None:intergenic | 45.0% | |
| GATTCGAAGAACGGAGTAAC+GGG | - | chr3.4:92336659-92336678 | None:intergenic | 45.0% | |
| GCAAACCTCCCTTTAATCCT+AGG | - | chr3.4:92337290-92337309 | None:intergenic | 45.0% | |
| GGATTCGAAGAACGGAGTAA+CGG | - | chr3.4:92336660-92336679 | None:intergenic | 45.0% | |
| GGCTAGGGTTATGAGGTATA+GGG | + | chr3.4:92337193-92337212 | MS.gene80166:CDS | 45.0% | |
| TGACTAATCTGCGACTGTGA+CGG | - | chr3.4:92336965-92336984 | None:intergenic | 45.0% | |
| TGGATCGAAGTTGCCATGAA+CGG | - | chr3.4:92336888-92336907 | None:intergenic | 45.0% | |
| TTCCGAAGCTATTCCGTTCA+TGG | + | chr3.4:92336817-92336836 | MS.gene80166:CDS | 45.0% | |
| ! | GATGCAGTTGACTCCATTTC+TGG | + | chr3.4:92337320-92337339 | MS.gene80166:CDS | 45.0% |
| ! | TTAGGTATCGTCTTCGTCGA+TGG | + | chr3.4:92337091-92337110 | MS.gene80166:intron | 45.0% |
| AGGTTGGAGTTGTTCCTGAC+GGG | + | chr3.4:92337114-92337133 | MS.gene80166:CDS | 50.0% | |
| ATCGAACTGTGGTTACGGGA+CGG | + | chr3.4:92337154-92337173 | MS.gene80166:CDS | 50.0% | |
| ATCGTGAGGCTAGGGTTATG+AGG | + | chr3.4:92337186-92337205 | MS.gene80166:CDS | 50.0% | |
| ATGAAGCTGAAGCTGCTTCG+TGG | + | chr3.4:92336741-92336760 | MS.gene80166:CDS | 50.0% | |
| CATATGCGGAAACTAGGCCT+AGG | + | chr3.4:92337270-92337289 | MS.gene80166:CDS | 50.0% | |
| CTAGGCCTAGGATTAAAGGG+AGG | + | chr3.4:92337282-92337301 | MS.gene80166:CDS | 50.0% | |
| GAGGTTGGAGTTGTTCCTGA+CGG | + | chr3.4:92337113-92337132 | MS.gene80166:CDS | 50.0% | |
| GATGCAGAGAGAAGCTGCAT+CGG | - | chr3.4:92336579-92336598 | None:intergenic | 50.0% | |
| GCTTTACACGTGACATGTGC+AGG | - | chr3.4:92336557-92336576 | None:intergenic | 50.0% | |
| GGATCGAAGTTGCCATGAAC+GGG | - | chr3.4:92336887-92336906 | None:intergenic | 50.0% | |
| GTGTCGGAGATATCGAACTG+TGG | + | chr3.4:92337143-92337162 | MS.gene80166:CDS | 50.0% | |
| TTTGTTAGCGGCGTGGACTT+TGG | - | chr3.4:92336483-92336502 | None:intergenic | 50.0% | |
| AGTGTGGATGTCACGGTCAC+AGG | - | chr3.4:92336603-92336622 | None:intergenic | 55.0% | |
| GGTATCGTCTTCGTCGATGG+AGG | + | chr3.4:92337094-92337113 | MS.gene80166:intron | 55.0% | |
| TCGTCTTCGTCGATGGAGGT+TGG | + | chr3.4:92337098-92337117 | MS.gene80166:CDS | 55.0% | |
| !! | ACGGGAGGCGAGTTTGTTAG+CGG | - | chr3.4:92336495-92336514 | None:intergenic | 55.0% |
| !! | GACGGTGTTGTTCCCGTTCA+TGG | + | chr3.4:92336872-92336891 | MS.gene80166:CDS | 55.0% |
| ACTTCGCAGAGAGTGACGCG+AGG | - | chr3.4:92336521-92336540 | None:intergenic | 60.0% | |
| AGAGAGTGACGCGAGGGTGA+CGG | - | chr3.4:92336514-92336533 | None:intergenic | 60.0% | |
| AGGGCGGCAGTAGAGAGTAG+CGG | - | chr3.4:92336429-92336448 | None:intergenic | 60.0% | |
| CTTCGCAGAGAGTGACGCGA+GGG | - | chr3.4:92336520-92336539 | None:intergenic | 60.0% | |
| GAACTGTGGTTACGGGACGG+TGG | + | chr3.4:92337157-92337176 | MS.gene80166:CDS | 60.0% | |
| GCAGTAGAGAGTAGCGGTGG+CGG | - | chr3.4:92336423-92336442 | None:intergenic | 60.0% | |
| TCCTGACGGGAACACCGTGT+CGG | + | chr3.4:92337127-92337146 | MS.gene80166:CDS | 60.0% | |
| ! | CGGCGAGAGGATTAGCAGTG+TGG | - | chr3.4:92336619-92336638 | None:intergenic | 60.0% |
| !! | AGGCGAGTTTGTTAGCGGCG+TGG | - | chr3.4:92336490-92336509 | None:intergenic | 60.0% |
| !! | GGCGTGGACTTTGGAGTCAC+AGG | - | chr3.4:92336474-92336493 | None:intergenic | 60.0% |
| AGAGGAAGGCGGAGTCAGGG+CGG | - | chr3.4:92336445-92336464 | None:intergenic | 65.0% | |
| CCTGACTCCGCCTTCCTCTG+CGG | + | chr3.4:92336446-92336465 | MS.gene80166:CDS | 65.0% | |
| CGCAGAGGAAGGCGGAGTCA+GGG | - | chr3.4:92336448-92336467 | None:intergenic | 65.0% | |
| CTGTGGTTACGGGACGGTGG+CGG | + | chr3.4:92337160-92337179 | MS.gene80166:CDS | 65.0% | |
| GAGAGTGACGCGAGGGTGAC+GGG | - | chr3.4:92336513-92336532 | None:intergenic | 65.0% | |
| GCGGCAGTAGAGAGTAGCGG+TGG | - | chr3.4:92336426-92336445 | None:intergenic | 65.0% | |
| GGGAACACGCTCGTGACGTG+CGG | - | chr3.4:92336639-92336658 | None:intergenic | 65.0% | |
| TCCGACACGGTGTTCCCGTC+AGG | - | chr3.4:92337131-92337150 | None:intergenic | 65.0% | |
| CCGCAGAGGAAGGCGGAGTC+AGG | - | chr3.4:92336449-92336468 | None:intergenic | 70.0% | |
| GGCGGCGGATCGTGAGGCTA+GGG | + | chr3.4:92337178-92337197 | MS.gene80166:CDS | 70.0% | |
| TGGCGGCGGATCGTGAGGCT+AGG | + | chr3.4:92337177-92337196 | MS.gene80166:CDS | 70.0% | |
| ! | ACAGGCGCCGCAGAGGAAGG+CGG | - | chr3.4:92336456-92336475 | None:intergenic | 70.0% |
| ! | GTCACAGGCGCCGCAGAGGA+AGG | - | chr3.4:92336459-92336478 | None:intergenic | 70.0% |
| ! | TGGAGTCACAGGCGCCGCAG+AGG | - | chr3.4:92336463-92336482 | None:intergenic | 70.0% |
| ! | TGGTTACGGGACGGTGGCGG+CGG | + | chr3.4:92337163-92337182 | MS.gene80166:CDS | 70.0% |
| !! | AGTGACGCGAGGGTGACGGG+AGG | - | chr3.4:92336510-92336529 | None:intergenic | 70.0% |
| CGCTCGTGACGTGCGGCGAG+AGG | - | chr3.4:92336632-92336651 | None:intergenic | 75.0% | |
| ! | GACGGTGGCGGCGGATCGTG+AGG | + | chr3.4:92337172-92337191 | MS.gene80166:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 92336389 | 92337367 | 92336389 | ID=MS.gene80166 |
| chr3.4 | mRNA | 92336389 | 92337367 | 92336389 | ID=MS.gene80166.t1;Parent=MS.gene80166 |
| chr3.4 | exon | 92336389 | 92336988 | 92336389 | ID=MS.gene80166.t1.exon1;Parent=MS.gene80166.t1 |
| chr3.4 | CDS | 92336389 | 92336988 | 92336389 | ID=cds.MS.gene80166.t1;Parent=MS.gene80166.t1 |
| chr3.4 | exon | 92337095 | 92337367 | 92337095 | ID=MS.gene80166.t1.exon2;Parent=MS.gene80166.t1 |
| chr3.4 | CDS | 92337095 | 92337367 | 92337095 | ID=cds.MS.gene80166.t1;Parent=MS.gene80166.t1 |
| Gene Sequence |
| Protein sequence |
