AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80295

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80295.t1 MTR_0558s0020 86.486 296 39 1 1 295 1 296 0.0 541
MS.gene80295.t1 MTR_1g083640 82.042 284 35 1 1 268 1 284 4.97e-173 489
MS.gene80295.t1 MTR_8g446020 73.220 295 40 1 1 295 1 256 2.22e-154 440
MS.gene80295.t1 MTR_2g014100 81.224 245 37 1 1 236 1 245 1.18e-143 413
MS.gene80295.t1 MTR_6g090505 81.857 237 43 0 65 301 1 237 2.01e-142 414
MS.gene80295.t1 MTR_6g090505 67.647 102 6 2 396 495 230 306 1.43e-34 134
MS.gene80295.t1 MTR_0337s0040 71.154 260 35 2 1 259 1 221 6.07e-125 364
MS.gene80295.t1 MTR_3g008240 82.759 203 34 1 1 202 1 203 2.72e-118 347
MS.gene80295.t1 MTR_4g040430 82.178 202 32 1 1 202 1 198 2.60e-117 344
MS.gene80295.t1 MTR_5g071590 71.823 181 38 2 1 168 1 181 5.55e-90 273
MS.gene80295.t1 MTR_1g055240 85.211 142 21 0 91 232 3 144 1.14e-85 261
MS.gene80295.t1 MTR_0019s0290 79.605 152 31 0 82 233 9 160 8.37e-85 259
MS.gene80295.t1 MTR_1281s0010 79.817 109 22 0 19 127 1 109 3.65e-57 186
MS.gene80295.t1 MTR_6g051425 30.818 318 189 6 40 341 22 324 1.50e-37 141
MS.gene80295.t1 MTR_0337s0060 91.045 67 6 0 447 513 21 87 2.36e-35 132
MS.gene80295.t1 MTR_4g008820 71.717 99 27 1 1 98 125 223 3.93e-33 134
MS.gene80295.t1 MTR_8g046060 78.409 88 10 2 1 79 91 178 1.15e-32 124
MS.gene80295.t1 MTR_1g070355 25.767 326 217 8 41 362 80 384 6.68e-18 87.0
MS.gene80295.t1 MTR_0402s0040 26.486 185 117 4 49 233 40 205 2.36e-14 75.9
MS.gene80295.t1 MTR_4g035820 22.759 290 191 7 17 299 61 324 3.95e-14 75.5
MS.gene80295.t1 MTR_0337s0050 75.000 36 9 0 259 294 14 49 1.51e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80295.t1 AT4G38180 28.298 470 314 6 32 499 70 518 1.84e-60 213
MS.gene80295.t1 AT3G06250 26.709 468 271 10 34 498 189 587 2.02e-42 161
MS.gene80295.t1 AT3G06250 26.709 468 271 10 34 498 189 587 2.02e-42 161
MS.gene80295.t1 AT3G06250 26.709 468 271 10 34 498 189 587 2.02e-42 161
MS.gene80295.t1 AT4G15090 25.325 462 316 9 50 507 66 502 2.26e-41 159
MS.gene80295.t1 AT4G15090 25.325 462 316 9 50 507 66 502 2.26e-41 159
MS.gene80295.t1 AT4G15090 25.325 462 316 9 50 507 66 502 2.26e-41 159
MS.gene80295.t1 AT4G15090 25.325 462 316 9 50 507 66 502 2.26e-41 159
MS.gene80295.t1 AT4G15090 25.325 462 316 9 50 507 66 502 2.26e-41 159
MS.gene80295.t1 AT1G76320 26.269 453 292 11 50 496 12 428 5.06e-41 157
MS.gene80295.t1 AT1G76320 26.269 453 292 11 50 496 12 428 1.02e-40 156
MS.gene80295.t1 AT1G76320 26.269 453 292 11 50 496 12 428 1.02e-40 156
MS.gene80295.t1 AT1G76320 26.269 453 292 11 50 496 12 428 1.21e-40 156
MS.gene80295.t1 AT4G38170 28.623 276 186 4 235 507 24 291 9.93e-38 146
MS.gene80295.t1 AT1G10240 26.226 469 299 14 49 497 62 503 1.38e-37 147
MS.gene80295.t1 AT1G10240 26.226 469 299 14 49 497 62 503 1.38e-37 147
MS.gene80295.t1 AT2G27110 26.790 377 251 7 127 499 108 463 5.99e-37 145
MS.gene80295.t1 AT2G27110 26.790 377 251 7 127 499 108 463 5.99e-37 145
MS.gene80295.t1 AT5G18960 25.208 480 286 12 24 498 200 611 6.19e-37 145
MS.gene80295.t1 AT5G18960 25.208 480 286 12 24 498 200 611 6.19e-37 145
MS.gene80295.t1 AT2G27110 29.066 289 197 3 213 499 36 318 1.16e-36 144
MS.gene80295.t1 AT5G28530 26.304 441 281 12 40 462 60 474 4.05e-36 142
MS.gene80295.t1 AT5G28530 26.304 441 281 12 40 462 60 474 4.44e-36 142
MS.gene80295.t1 AT5G28530 26.304 441 281 12 40 462 60 474 4.44e-36 142
MS.gene80295.t1 AT3G22170 22.634 486 321 11 34 507 69 511 5.48e-34 137
MS.gene80295.t1 AT3G22170 22.634 486 321 11 34 507 69 511 5.48e-34 137
MS.gene80295.t1 AT2G32250 24.056 503 302 13 1 496 8 437 2.83e-32 131
MS.gene80295.t1 AT2G32250 24.056 503 302 13 1 496 8 437 3.75e-32 131
MS.gene80295.t1 AT2G32250 24.056 503 302 13 1 496 8 437 3.75e-32 131
MS.gene80295.t1 AT2G32250 24.056 503 302 13 1 496 8 437 3.75e-32 131
MS.gene80295.t1 AT2G32250 24.056 503 302 13 1 496 8 437 3.91e-32 131
MS.gene80295.t1 AT2G32250 24.056 503 302 13 1 496 8 437 3.91e-32 131
MS.gene80295.t1 AT2G32250 24.449 454 267 11 50 496 12 396 1.02e-31 130
MS.gene80295.t1 AT2G32250 24.449 454 267 11 50 496 12 396 1.18e-31 130
MS.gene80295.t1 AT1G80010 25.855 468 268 17 41 478 101 519 1.73e-31 129
MS.gene80295.t1 AT1G52520 23.125 480 322 13 41 512 91 531 6.94e-29 121
MS.gene80295.t1 AT4G19990 24.009 454 280 12 41 483 27 426 7.14e-28 118
MS.gene80295.t1 AT4G19990 24.009 454 280 12 41 483 27 426 7.14e-28 118
MS.gene80295.t1 AT4G19990 24.009 454 280 12 41 483 27 426 7.46e-28 118
MS.gene80295.t1 AT4G19990 24.009 454 280 12 41 483 41 440 7.84e-28 118
MS.gene80295.t1 AT4G19990 24.009 454 280 12 41 483 27 426 1.07e-27 117
MS.gene80295.t1 AT4G19990 24.009 454 280 12 41 483 27 426 1.07e-27 117
MS.gene80295.t1 AT4G19990 28.358 268 185 4 219 483 106 369 1.41e-27 117

Find 74 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCGATGAGTTAACTAATT+TGG 0.156761 6.1:+59693435 MS.gene80295:CDS
CTTTAGTCATTTCAACATTA+TGG 0.194287 6.1:+59693702 MS.gene80295:CDS
TACATTTCAGCTGCCTTTCT+TGG 0.197151 6.1:+59693154 MS.gene80295:intron
CAACAAGATTATGCTCTATT+TGG 0.198611 6.1:+59692668 MS.gene80295:CDS
CATCGTAAGAGTGTGTTATT+TGG 0.276792 6.1:+59693187 MS.gene80295:CDS
TATGGTTTGAGGTTTCTTAT+TGG 0.280533 6.1:-59693281 None:intergenic
AATCAGATTTCGACTACAAT+TGG 0.318540 6.1:+59693476 MS.gene80295:CDS
ACCAGCCTGGTCTGTGTATA+TGG 0.341000 6.1:-59693299 None:intergenic
GCCGATATTTATTGTTTGTT+CGG 0.341758 6.1:-59692718 None:intergenic
GAGGGATAATCATGCTGATT+TGG 0.346844 6.1:+59693735 MS.gene80295:CDS
CTCAAGTAGCGCAAATGCTT+TGG 0.369487 6.1:-59692335 None:intergenic
TATGCATTGGTCTTCAGTTT+GGG 0.370760 6.1:+59693576 MS.gene80295:CDS
TTCTCATACTCAACCAGAAC+TGG 0.385591 6.1:-59692188 None:intergenic
AACAATAAATATCGGCCATT+AGG 0.388497 6.1:+59692725 MS.gene80295:CDS
TCATGCTGATTTGGACTTCA+AGG 0.389304 6.1:+59693744 MS.gene80295:CDS
ACCGAACAAACAATAAATAT+CGG 0.389395 6.1:+59692717 MS.gene80295:CDS
GTATGCATTGGTCTTCAGTT+TGG 0.392120 6.1:+59693575 MS.gene80295:CDS
CCATGATTCTTTATCACTAA+AGG 0.406174 6.1:-59691949 None:intergenic
TAAAGAATCATGGAAGTCTT+TGG 0.408500 6.1:+59691959 MS.gene80295:CDS
CGAGAAGTAAATAAAGTTCA+AGG 0.413853 6.1:+59692365 MS.gene80295:CDS
ATAACACTGGATTCTCTGTT+AGG 0.417808 6.1:+59692030 MS.gene80295:CDS
TGAAGCGTTGAATGTCTATA+AGG 0.418336 6.1:+59692271 MS.gene80295:CDS
AACATGTGGCAGTCCAGTTC+TGG 0.427990 6.1:+59692175 MS.gene80295:CDS
AGCTGGAATATATTTGAAGT+TGG 0.433136 6.1:+59692247 MS.gene80295:CDS
TGAATGTCTATAAGGTATAT+AGG 0.445219 6.1:+59692279 MS.gene80295:CDS
TGGTCTGTGTATATGGTTTG+AGG 0.447350 6.1:-59693292 None:intergenic
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 6.1:+59692230 MS.gene80295:CDS
CGTTATGCTTGTAACAAAGA+AGG 0.449439 6.1:+59692113 MS.gene80295:CDS
GCTTTAATAAATTCTAAAGC+TGG 0.450264 6.1:+59692395 MS.gene80295:CDS
ATCTGAGAGTGGACAATTGA+CGG 0.452529 6.1:+59691884 MS.gene80295:CDS
TGGTCTTTGCTCATGGCACA+TGG 0.452802 6.1:+59693366 MS.gene80295:CDS
GGCCTACTTCAGACTTTAAG+TGG 0.462107 6.1:+59693530 MS.gene80295:CDS
AAATGCTTTGGTTTATGTAA+TGG 0.467024 6.1:-59692323 None:intergenic
ACAAAATAAGAAATCGGATA+AGG 0.469178 6.1:+59692079 MS.gene80295:CDS
GAAGTGATGGGTCAAGGAGT+TGG 0.484292 6.1:+59692440 MS.gene80295:CDS
TTACAACCAACCCTTTCCTC+TGG 0.485330 6.1:-59692224 None:intergenic
TTTAATTATGCATATAACAC+TGG 0.485542 6.1:+59692017 MS.gene80295:CDS
TCTAATGACAACTCGTCAAA+AGG 0.488137 6.1:+59692508 MS.gene80295:CDS
CAATTCACAAAATAAGAAAT+CGG 0.490263 6.1:+59692073 MS.gene80295:CDS
ATGATTGTCAAAACCAAGAA+AGG 0.491378 6.1:-59693167 None:intergenic
ATCAGATTTCGACTACAATT+GGG 0.491708 6.1:+59693477 MS.gene80295:CDS
CGTCAAAAGGAGATGGTGGC+TGG 0.492100 6.1:+59692521 MS.gene80295:CDS
ACATTATGGTTCAGAGAATG+AGG 0.494022 6.1:+59693716 MS.gene80295:CDS
GACCGCATAAGCTGACTGTT+GGG 0.496307 6.1:-59693796 None:intergenic
GAGAACATCTTATGAAGTGA+TGG 0.499875 6.1:+59692427 MS.gene80295:CDS
TGTCTTCTGCAGCATCAACT+TGG 0.502977 6.1:-59692613 None:intergenic
CTCATTCAAATCGTATGCAT+TGG 0.507227 6.1:+59693563 MS.gene80295:CDS
TTTAAGTCCGAAAATCGAAA+AGG 0.508080 6.1:-59692479 None:intergenic
ATTGGTCTTCAGTTTGGGTG+AGG 0.509410 6.1:+59693581 MS.gene80295:CDS
TAAAGAATCGAACAAGTGAA+TGG 0.519618 6.1:-59693684 None:intergenic
TGTCTATAAGGTATATAGGT+GGG 0.530085 6.1:+59692283 MS.gene80295:CDS
TAACAAAGAAGGTTTCAAGA+AGG 0.538422 6.1:+59692124 MS.gene80295:CDS
AATCGAACAAGTGAATGGTC+TGG 0.540971 6.1:-59693679 None:intergenic
CCTTTAGTGATAAAGAATCA+TGG 0.543522 6.1:+59691949 MS.gene80295:CDS
AACAAAGAAGGTTTCAAGAA+GGG 0.545537 6.1:+59692125 MS.gene80295:CDS
ACTTCAACAACGCACCAGCC+TGG 0.557139 6.1:-59693312 None:intergenic
TGAAAATTCAGTTATTGATG+CGG 0.565546 6.1:+59691908 MS.gene80295:CDS
GTGATGGGTCAAGGAGTTGG+AGG 0.568802 6.1:+59692443 MS.gene80295:CDS
GGACCGCATAAGCTGACTGT+TGG 0.579264 6.1:-59693797 None:intergenic
TCTTATGAAGTGATGGGTCA+AGG 0.589029 6.1:+59692434 MS.gene80295:CDS
ACCATATACACAGACCAGGC+TGG 0.590176 6.1:+59693298 MS.gene80295:CDS
GACAACTCGTCAAAAGGAGA+TGG 0.599038 6.1:+59692514 MS.gene80295:CDS
AGAACATCTTATGAAGTGAT+GGG 0.601988 6.1:+59692428 MS.gene80295:CDS
AACTCGTCAAAAGGAGATGG+TGG 0.603142 6.1:+59692517 MS.gene80295:CDS
ATCCCAACAGTCAGCTTATG+CGG 0.606153 6.1:+59693794 MS.gene80295:CDS
AGAACTGGACTGCCACATGT+TGG 0.612393 6.1:-59692173 None:intergenic
ATATCAACTTCATCTGAGAG+TGG 0.614980 6.1:+59691873 MS.gene80295:CDS
AGCCACTTAAAGTCTGAAGT+AGG 0.617789 6.1:-59693532 None:intergenic
AATGAAGCTCTCTCAAAACC+AGG 0.618452 6.1:+59692575 MS.gene80295:CDS
GGGTCAAGGAGTTGGAGGCA+CGG 0.621882 6.1:+59692448 MS.gene80295:CDS
ATGTCTATAAGGTATATAGG+TGG 0.625332 6.1:+59692282 MS.gene80295:CDS
GGTCTTTGCTCATGGCACAT+GGG 0.639768 6.1:+59693367 MS.gene80295:CDS
CATTATGGTTCAGAGAATGA+GGG 0.643278 6.1:+59693717 MS.gene80295:CDS
TCAAACCATATACACAGACC+AGG 0.671057 6.1:+59693294 MS.gene80295:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAACACATTAAATTCTA+AGG - chr6.1:59692939-59692958 None:intergenic 15.0%
!! CAATTCACAAAATAAGAAAT+CGG + chr6.1:59692073-59692092 MS.gene80295:CDS 20.0%
!! TTTAATTATGCATATAACAC+TGG + chr6.1:59692017-59692036 MS.gene80295:CDS 20.0%
!!! AATTTAATGTTCTGGTTTAT+TGG + chr6.1:59692900-59692919 MS.gene80295:intron 20.0%
!!! ATGATGAAAAACTGTTTTAA+TGG + chr6.1:59693505-59693524 MS.gene80295:CDS 20.0%
!!! TGAATGTTTATATTGATTGA+AGG + chr6.1:59692872-59692891 MS.gene80295:intron 20.0%
! AAAACACCTTGAAAAACATT+CGG - chr6.1:59693343-59693362 None:intergenic 25.0%
! ACAAAATAAGAAATCGGATA+AGG + chr6.1:59692079-59692098 MS.gene80295:CDS 25.0%
! ACCGAACAAACAATAAATAT+CGG + chr6.1:59692717-59692736 MS.gene80295:CDS 25.0%
! AGTACAAAATACTAACCTAA+TGG - chr6.1:59692743-59692762 None:intergenic 25.0%
! CATAATAGTATTTGAAGGAA+AGG - chr6.1:59693876-59693895 None:intergenic 25.0%
! CTTTAGTCATTTCAACATTA+TGG + chr6.1:59693702-59693721 MS.gene80295:CDS 25.0%
! TGAAAATTCAGTTATTGATG+CGG + chr6.1:59691908-59691927 MS.gene80295:CDS 25.0%
! TGAAATTTGAATGAATGTTC+TGG + chr6.1:59692790-59692809 MS.gene80295:intron 25.0%
! TGAATAAATGAAAAGCAAGA+TGG - chr6.1:59693841-59693860 None:intergenic 25.0%
! TGAATGTCTATAAGGTATAT+AGG + chr6.1:59692279-59692298 MS.gene80295:CDS 25.0%
! TTCCTTCAAATACTATTATG+AGG + chr6.1:59693876-59693895 MS.gene80295:intron 25.0%
! TTTCGATGAGTTAACTAATT+TGG + chr6.1:59693435-59693454 MS.gene80295:CDS 25.0%
!! AAATGCTTTGGTTTATGTAA+TGG - chr6.1:59692326-59692345 None:intergenic 25.0%
!! AGGTTTAGAATTTAATGTTC+TGG + chr6.1:59692892-59692911 MS.gene80295:intron 25.0%
!! TGTTGTATTTTGTTCTGATT+TGG + chr6.1:59693125-59693144 MS.gene80295:intron 25.0%
!!! AAGACATAGCTAGTATTTTT+TGG + chr6.1:59692630-59692649 MS.gene80295:CDS 25.0%
!!! AGACATAGCTAGTATTTTTT+GGG + chr6.1:59692631-59692650 MS.gene80295:CDS 25.0%
!!! ATGTTGATAATGATAATGAG+AGG + chr6.1:59693977-59693996 MS.gene80295:intron 25.0%
!!! CTTATTTTGTGAATTGTTCT+TGG - chr6.1:59692069-59692088 None:intergenic 25.0%
!!! GCTTTAATAAATTCTAAAGC+TGG + chr6.1:59692395-59692414 MS.gene80295:CDS 25.0%
!!! TGAAAAACTGTTTTAATGGA+AGG + chr6.1:59693509-59693528 MS.gene80295:CDS 25.0%
!!! TGTTGATAATGATAATGAGA+GGG + chr6.1:59693978-59693997 MS.gene80295:intron 25.0%
AACAAAGAAGGTTTCAAGAA+GGG + chr6.1:59692125-59692144 MS.gene80295:CDS 30.0%
AACAATAAATATCGGCCATT+AGG + chr6.1:59692725-59692744 MS.gene80295:CDS 30.0%
AATCAGATTTCGACTACAAT+TGG + chr6.1:59693476-59693495 MS.gene80295:CDS 30.0%
AGAACATCTTATGAAGTGAT+GGG + chr6.1:59692428-59692447 MS.gene80295:CDS 30.0%
ATCAGATTTCGACTACAATT+GGG + chr6.1:59693477-59693496 MS.gene80295:CDS 30.0%
ATGATTGTCAAAACCAAGAA+AGG - chr6.1:59693170-59693189 None:intergenic 30.0%
ATGTCTATAAGGTATATAGG+TGG + chr6.1:59692282-59692301 MS.gene80295:CDS 30.0%
CAACAAGATTATGCTCTATT+TGG + chr6.1:59692668-59692687 MS.gene80295:CDS 30.0%
CCATGATTCTTTATCACTAA+AGG - chr6.1:59691952-59691971 None:intergenic 30.0%
CCTTTAGTGATAAAGAATCA+TGG + chr6.1:59691949-59691968 MS.gene80295:CDS 30.0%
CGAGAAGTAAATAAAGTTCA+AGG + chr6.1:59692365-59692384 MS.gene80295:CDS 30.0%
CTCCTCATAATAGTATTTGA+AGG - chr6.1:59693881-59693900 None:intergenic 30.0%
CTGGTATATGTGTGTTAATT+TGG + chr6.1:59692829-59692848 MS.gene80295:intron 30.0%
CTTCAAATACTATTATGAGG+AGG + chr6.1:59693879-59693898 MS.gene80295:intron 30.0%
GAGAATGCAAAGAAAAATCT+CGG + chr6.1:59693391-59693410 MS.gene80295:CDS 30.0%
GCCGATATTTATTGTTTGTT+CGG - chr6.1:59692721-59692740 None:intergenic 30.0%
TAAAGAAAAACCAGAGGAAA+GGG + chr6.1:59692214-59692233 MS.gene80295:CDS 30.0%
TAAAGAATCATGGAAGTCTT+TGG + chr6.1:59691959-59691978 MS.gene80295:CDS 30.0%
TAAAGAATCGAACAAGTGAA+TGG - chr6.1:59693687-59693706 None:intergenic 30.0%
TAACAAAGAAGGTTTCAAGA+AGG + chr6.1:59692124-59692143 MS.gene80295:CDS 30.0%
TGGATATCATAGTAAAAACC+TGG - chr6.1:59692596-59692615 None:intergenic 30.0%
TGGTGTATACTTGTTTACTA+CGG - chr6.1:59693821-59693840 None:intergenic 30.0%
TGTCTATAAGGTATATAGGT+GGG + chr6.1:59692283-59692302 MS.gene80295:CDS 30.0%
TTTAAGTCCGAAAATCGAAA+AGG - chr6.1:59692482-59692501 None:intergenic 30.0%
! AGCTGGAATATATTTGAAGT+TGG + chr6.1:59692247-59692266 MS.gene80295:CDS 30.0%
! CAAAGTTTTCACAATTGAGA+AGG + chr6.1:59693936-59693955 MS.gene80295:intron 30.0%
! GAATCTTCCTTTTCGATTTT+CGG + chr6.1:59692472-59692491 MS.gene80295:CDS 30.0%
! GGTTTCAAATGTTGATGTTT+TGG + chr6.1:59693999-59694018 MS.gene80295:intron 30.0%
! TATGGTTTGAGGTTTCTTAT+TGG - chr6.1:59693284-59693303 None:intergenic 30.0%
! TCAATTCCGAATGTTTTTCA+AGG + chr6.1:59693334-59693353 MS.gene80295:CDS 30.0%
!! ATGTTCTGGTTTAGAATTGA+AGG + chr6.1:59692804-59692823 MS.gene80295:intron 30.0%
!!! GTTTTTCAAGGTGTTTTTCA+TGG + chr6.1:59693346-59693365 MS.gene80295:CDS 30.0%
ACATTATGGTTCAGAGAATG+AGG + chr6.1:59693716-59693735 MS.gene80295:CDS 35.0%
ATAACACTGGATTCTCTGTT+AGG + chr6.1:59692030-59692049 MS.gene80295:CDS 35.0%
ATATCAACTTCATCTGAGAG+TGG + chr6.1:59691873-59691892 MS.gene80295:CDS 35.0%
CATTATGGTTCAGAGAATGA+GGG + chr6.1:59693717-59693736 MS.gene80295:CDS 35.0%
CGTTATGCTTGTAACAAAGA+AGG + chr6.1:59692113-59692132 MS.gene80295:CDS 35.0%
CTAAAGAAAAACCAGAGGAA+AGG + chr6.1:59692213-59692232 MS.gene80295:CDS 35.0%
CTCATTCAAATCGTATGCAT+TGG + chr6.1:59693563-59693582 MS.gene80295:CDS 35.0%
GAAAGCTAAAGAAAAACCAG+AGG + chr6.1:59692208-59692227 MS.gene80295:CDS 35.0%
GAGAACATCTTATGAAGTGA+TGG + chr6.1:59692427-59692446 MS.gene80295:CDS 35.0%
GTCTGCTTAATCCTAAAAAC+AGG + chr6.1:59692150-59692169 MS.gene80295:CDS 35.0%
TCTAATGACAACTCGTCAAA+AGG + chr6.1:59692508-59692527 MS.gene80295:CDS 35.0%
! CATCGTAAGAGTGTGTTATT+TGG + chr6.1:59693187-59693206 MS.gene80295:CDS 35.0%
! TCTTTCAATGCTTTTCTTCG+TGG + chr6.1:59693646-59693665 MS.gene80295:CDS 35.0%
! TGGTTTAGAATTGAAGGTTC+TGG + chr6.1:59692810-59692829 MS.gene80295:intron 35.0%
! TTTTTCATGGTCTTTGCTCA+TGG + chr6.1:59693359-59693378 MS.gene80295:CDS 35.0%
!! TATGCATTGGTCTTCAGTTT+GGG + chr6.1:59693576-59693595 MS.gene80295:CDS 35.0%
!! TGAAGCGTTGAATGTCTATA+AGG + chr6.1:59692271-59692290 MS.gene80295:CDS 35.0%
!!! AGACATCAGCTACTTTTGAT+TGG + chr6.1:59693230-59693249 MS.gene80295:CDS 35.0%
AATCGAACAAGTGAATGGTC+TGG - chr6.1:59693682-59693701 None:intergenic 40.0%
AATGAAGCTCTCTCAAAACC+AGG + chr6.1:59692575-59692594 MS.gene80295:CDS 40.0%
ACCTATGATCTTTGCATAGC+TGG - chr6.1:59693034-59693053 None:intergenic 40.0%
ATCTGAGAGTGGACAATTGA+CGG + chr6.1:59691884-59691903 MS.gene80295:CDS 40.0%
ATGAGGAGGACACAACAAAA+GGG + chr6.1:59693893-59693912 MS.gene80295:intron 40.0%
TACATTTCAGCTGCCTTTCT+TGG + chr6.1:59693154-59693173 MS.gene80295:intron 40.0%
TATGAGGAGGACACAACAAA+AGG + chr6.1:59693892-59693911 MS.gene80295:intron 40.0%
TCAAACCATATACACAGACC+AGG + chr6.1:59693294-59693313 MS.gene80295:CDS 40.0%
TCCAGCTATGCAAAGATCAT+AGG + chr6.1:59693030-59693049 MS.gene80295:intron 40.0%
TGGTCTGTGTATATGGTTTG+AGG - chr6.1:59693295-59693314 None:intergenic 40.0%
TTCTCATACTCAACCAGAAC+TGG - chr6.1:59692191-59692210 None:intergenic 40.0%
! AGCCACTTAAAGTCTGAAGT+AGG - chr6.1:59693535-59693554 None:intergenic 40.0%
! GAGGGATAATCATGCTGATT+TGG + chr6.1:59693735-59693754 MS.gene80295:CDS 40.0%
! GTGAGGTCACATTTTACAAC+TGG + chr6.1:59693598-59693617 MS.gene80295:CDS 40.0%
!! GTATGCATTGGTCTTCAGTT+TGG + chr6.1:59693575-59693594 MS.gene80295:CDS 40.0%
!! TCATGCTGATTTGGACTTCA+AGG + chr6.1:59693744-59693763 MS.gene80295:CDS 40.0%
!! TCTTATGAAGTGATGGGTCA+AGG + chr6.1:59692434-59692453 MS.gene80295:CDS 40.0%
!!! GCTAGTATTTTTTGGGCAGA+TGG + chr6.1:59692638-59692657 MS.gene80295:CDS 40.0%
AACTCGTCAAAAGGAGATGG+TGG + chr6.1:59692517-59692536 MS.gene80295:CDS 45.0%
ATCCCAACAGTCAGCTTATG+CGG + chr6.1:59693794-59693813 MS.gene80295:CDS 45.0%
CCTAAAAACAGGCCAACATG+TGG + chr6.1:59692161-59692180 MS.gene80295:CDS 45.0%
CTCAAGTAGCGCAAATGCTT+TGG - chr6.1:59692338-59692357 None:intergenic 45.0%
GAAAAACCAGAGGAAAGGGT+TGG + chr6.1:59692218-59692237 MS.gene80295:CDS 45.0%
GACAACTCGTCAAAAGGAGA+TGG + chr6.1:59692514-59692533 MS.gene80295:CDS 45.0%
GGCCTACTTCAGACTTTAAG+TGG + chr6.1:59693530-59693549 MS.gene80295:CDS 45.0%
TGTCTTCTGCAGCATCAACT+TGG - chr6.1:59692616-59692635 None:intergenic 45.0%
TTACAACCAACCCTTTCCTC+TGG - chr6.1:59692227-59692246 None:intergenic 45.0%
!! ATTGGTCTTCAGTTTGGGTG+AGG + chr6.1:59693581-59693600 MS.gene80295:CDS 45.0%
!! CCACATGTTGGCCTGTTTTT+AGG - chr6.1:59692164-59692183 None:intergenic 45.0%
!! GAAAGGGTTGGTTGTAAAGC+TGG + chr6.1:59692230-59692249 MS.gene80295:CDS 45.0%
AACATGTGGCAGTCCAGTTC+TGG + chr6.1:59692175-59692194 MS.gene80295:CDS 50.0%
ACCAGCCTGGTCTGTGTATA+TGG - chr6.1:59693302-59693321 None:intergenic 50.0%
ACCATATACACAGACCAGGC+TGG + chr6.1:59693298-59693317 MS.gene80295:CDS 50.0%
AGAACTGGACTGCCACATGT+TGG - chr6.1:59692176-59692195 None:intergenic 50.0%
GACCGCATAAGCTGACTGTT+GGG - chr6.1:59693799-59693818 None:intergenic 50.0%
GGTCTTTGCTCATGGCACAT+GGG + chr6.1:59693367-59693386 MS.gene80295:CDS 50.0%
TGGTCTTTGCTCATGGCACA+TGG + chr6.1:59693366-59693385 MS.gene80295:CDS 50.0%
!! GAAGTGATGGGTCAAGGAGT+TGG + chr6.1:59692440-59692459 MS.gene80295:CDS 50.0%
ACTTCAACAACGCACCAGCC+TGG - chr6.1:59693315-59693334 None:intergenic 55.0%
GGACCGCATAAGCTGACTGT+TGG - chr6.1:59693800-59693819 None:intergenic 55.0%
! CGTCAAAAGGAGATGGTGGC+TGG + chr6.1:59692521-59692540 MS.gene80295:CDS 55.0%
!! GTGATGGGTCAAGGAGTTGG+AGG + chr6.1:59692443-59692462 MS.gene80295:CDS 55.0%
GGGTCAAGGAGTTGGAGGCA+CGG + chr6.1:59692448-59692467 MS.gene80295:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 59691864 59694034 59691864 ID=MS.gene80295
chr6.1 mRNA 59691864 59694034 59691864 ID=MS.gene80295.t1;Parent=MS.gene80295
chr6.1 exon 59691864 59692746 59691864 ID=MS.gene80295.t1.exon1;Parent=MS.gene80295.t1
chr6.1 CDS 59691864 59692746 59691864 ID=cds.MS.gene80295.t1;Parent=MS.gene80295.t1
chr6.1 exon 59693164 59693815 59693164 ID=MS.gene80295.t1.exon2;Parent=MS.gene80295.t1
chr6.1 CDS 59693164 59693815 59693164 ID=cds.MS.gene80295.t1;Parent=MS.gene80295.t1
chr6.1 exon 59694028 59694034 59694028 ID=MS.gene80295.t1.exon3;Parent=MS.gene80295.t1
chr6.1 CDS 59694028 59694034 59694028 ID=cds.MS.gene80295.t1;Parent=MS.gene80295.t1
Gene Sequence

>MS.gene80295

ATGGATTTGATATCAACTTCATCTGAGAGTGGACAATTGACGGTTGAAAATTCAGTTATTGATGCGGATAGTTCTCAATGTTACTCCTTTAGTGATAAAGAATCATGGAAGTCTTTGGTCTTTAAATCTATAAAAGAGATTGAAGATTTTTATTTTAATTATGCATATAACACTGGATTCTCTGTTAGGAGTATGTTGAAGTCCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGCTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGGCAGTCCAGTTCTGGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGGTATATAGGTGGGAAGTTGATCACTGTCATCCATTACATAAACCAAAGCATTTGCGCTACTTGAGATCATTTCGAGAAGTAAATAAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTCAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACAACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCAAAACCAGGTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCTAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTGGTGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGGTTAGTATTTTGTACTTCTCACAGTTTTTAATTCTTATATTCTTGAAATTTGAATGAATGTTCTGGTTTAGAATTGAAGGTTCTGGTATATGTGTGTTAATTTGGCTCTTGTGTGTGTTTAGAATTGAATGTTTATATTGATTGAAGGTTTAGAATTTAATGTTCTGGTTTATTGGTGATCTTATCTTGCCTTAGAATTTAATGTGTTTTTTTATCGATGATAACTAAATAGCTCTTTCTGTGTTTGCGAGTTTAGACATCTCGCTTGAGAAGTTGTGCTATGTCCAGCTATGCAAAGATCATAGGTGTTTTTTTACAGTTGTTAAATGAATAGCTTTTTCTATTTACACAGCACAATTGTTTTATATTTTTTAAATATGTTGTATTTTGTTCTGATTTGGTTCATTTACATTTCAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTCCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAATGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTAATGGAAGGCCTACTTCAGACTTTAAGTGGCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAACTGGTTTGAAAACGACTCAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGATTTGGACTTCAAGGCTGCAAACACTAGAACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACACAACAAAAGGGTCTGAATTGAACAAGTTCTTCAAAGTTTTCACAATTGAGAAGGTTGATTATAATTACGACGATGTTGATAATGATAATGAGAGGGTTTCAAATGTTGATGTTTTGGATGCAGAACTTGA
Protein sequence

>MS.gene80295.t1

MDLISTSSESGQLTVENSVIDADSSQCYSFSDKESWKSLVFKSIKEIEDFYFNYAYNTGFSVRSMLKSKNNSQNKKSDKVHYVRYACNKEGFKKGSLLNPKNRPTCGSPVLVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKVYRWEVDHCHPLHKPKHLRYLRSFREVNKVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPGFYYDIQVDAAEDIASIFWADGIMQQDYALFGDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGALLKSIPNVFQGVFHGLCSWHMGENAKKNLGSRANNAFFDELTNLVSNVEDESDFDYNWDQMMKNCFNGRPTSDFKWLVQTHSNRMHWSSVWVRSHFTTGLKTTQLSESFNAFLRGFLQPDHSLVRFFSHFNIMVQRMRDNHADLDFKAANTRTKNNYPNSQLMRT