Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80404.t1 | RHN43809.1 | 70 | 80 | 9 | 2 | 26 | 100 | 56 | 125 | 2.80E-16 | 95.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80404.t1 | Q9ZT96 | 46.8 | 77 | 31 | 1 | 24 | 100 | 116 | 182 | 2.5e-10 | 67.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80404.t1 | G7LBF2 | 70.0 | 80 | 9 | 2 | 26 | 100 | 56 | 125 | 2.0e-16 | 95.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene80404.t1 | TR | mTERF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80404.t1 | MTR_1g115415 | 82.584 | 178 | 5 | 1 | 26 | 177 | 78 | 255 | 1.50e-91 | 276 |
| MS.gene80404.t1 | MTR_8g105560 | 79.268 | 164 | 19 | 2 | 19 | 177 | 49 | 202 | 2.33e-78 | 244 |
| MS.gene80404.t1 | MTR_5g068860 | 46.000 | 200 | 76 | 2 | 3 | 176 | 93 | 286 | 2.85e-47 | 162 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80404.t1 | AT2G44020 | 71.910 | 178 | 24 | 1 | 26 | 177 | 83 | 260 | 2.64e-79 | 245 |
| MS.gene80404.t1 | AT4G02990 | 50.000 | 182 | 65 | 1 | 23 | 178 | 115 | 296 | 8.30e-53 | 177 |
| MS.gene80404.t1 | AT4G02990 | 50.000 | 182 | 65 | 1 | 23 | 178 | 115 | 296 | 8.30e-53 | 177 |
Find 40 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCTGTTATTAAGTTTCTT+AGG | 0.190808 | 6.3:+28804395 | MS.gene80404:CDS |
| CCCTGATGAATTGCAGGATT+TGG | 0.241129 | 6.3:+28804141 | MS.gene80404:CDS |
| TACCGAAGTGCAACGGTTCC+AGG | 0.289835 | 6.3:-28804012 | None:intergenic |
| CTTCGATTATCTCAAGGGTT+TGG | 0.298946 | 6.3:+28804111 | MS.gene80404:CDS |
| CTCATGACTTCAACGGTTGC+AGG | 0.306808 | 6.3:-28804169 | None:intergenic |
| TTCGATTATCTCAAGGGTTT+GGG | 0.314201 | 6.3:+28804112 | MS.gene80404:CDS |
| TCCTGTTATTAAGTTTCTTA+GGG | 0.320182 | 6.3:+28804396 | MS.gene80404:CDS |
| AGCTTAAACCCTAAAAGTTC+AGG | 0.348375 | 6.3:-28804466 | None:intergenic |
| CCCCTAAGAAACTTAATAAC+AGG | 0.351787 | 6.3:-28804397 | None:intergenic |
| ATGTAGAGAAAGATGATATT+GGG | 0.358260 | 6.3:+28804425 | MS.gene80404:CDS |
| TGATTACTTGGTTATTTACT+TGG | 0.371549 | 6.3:+28804621 | MS.gene80404:CDS |
| TTAGTATTGGTGTTAATCCT+AGG | 0.373470 | 6.3:+28804524 | MS.gene80404:CDS |
| GATGTAGAGAAAGATGATAT+TGG | 0.374971 | 6.3:+28804424 | MS.gene80404:CDS |
| GTGGCTTATCTTGTTAGTAT+TGG | 0.382878 | 6.3:+28804511 | MS.gene80404:CDS |
| AAAGCTTCTTGTTGATTACT+TGG | 0.384482 | 6.3:+28804609 | MS.gene80404:CDS |
| AATTATCCCTGATGAATTGC+AGG | 0.399920 | 6.3:+28804135 | MS.gene80404:CDS |
| TCCAAATCCTGCAATTCATC+AGG | 0.400283 | 6.3:-28804142 | None:intergenic |
| TCCTAGGGATATTGGTCCTA+TGG | 0.421118 | 6.3:+28804540 | MS.gene80404:CDS |
| AGGAAATCTGCTCCTACTAC+TGG | 0.446030 | 6.3:+28803961 | MS.gene80404:intron |
| GGTGTTAATCCTAGGGATAT+TGG | 0.451217 | 6.3:+28804532 | MS.gene80404:CDS |
| AAACCTCACCGTTACCGTCC+TGG | 0.463835 | 6.3:+28803994 | MS.gene80404:CDS |
| TATCATCTTCGATTATCTCA+AGG | 0.496441 | 6.3:+28804105 | MS.gene80404:CDS |
| AGGATTTCTAGGTCTAAACT+TGG | 0.506242 | 6.3:+28804316 | MS.gene80404:CDS |
| TAGTATTGGTGTTAATCCTA+GGG | 0.522188 | 6.3:+28804525 | MS.gene80404:CDS |
| ATCATCTTCGATTATCTCAA+GGG | 0.534022 | 6.3:+28804106 | MS.gene80404:CDS |
| CCTGTTATTAAGTTTCTTAG+GGG | 0.577517 | 6.3:+28804397 | MS.gene80404:CDS |
| ACACTAGCATGCAAAACTTG+TGG | 0.577739 | 6.3:-28804358 | None:intergenic |
| CTGCAACCGTTGAAGTCATG+AGG | 0.579492 | 6.3:+28804170 | MS.gene80404:CDS |
| CAACGGTTCCAGGACGGTAA+CGG | 0.580492 | 6.3:-28804002 | None:intergenic |
| ACGTTCCCTCATGACTTCAA+CGG | 0.586865 | 6.3:-28804176 | None:intergenic |
| TAAGGATACTGTGTAACCAT+AGG | 0.587849 | 6.3:-28804556 | None:intergenic |
| CCAAATCCTGCAATTCATCA+GGG | 0.619542 | 6.3:-28804141 | None:intergenic |
| ACGGAATTACCGAAGTGCAA+CGG | 0.619995 | 6.3:-28804019 | None:intergenic |
| AAATCTGCTCCTACTACTGG+AGG | 0.621448 | 6.3:+28803964 | MS.gene80404:CDS |
| AGGTACTATGAGTACTTCAG+TGG | 0.624393 | 6.3:+28804492 | MS.gene80404:CDS |
| GTCCTGGAACCGTTGCACTT+CGG | 0.635725 | 6.3:+28804010 | MS.gene80404:CDS |
| ACCATAGGACCAATATCCCT+AGG | 0.641670 | 6.3:-28804541 | None:intergenic |
| TGCAACCGTTGAAGTCATGA+GGG | 0.653234 | 6.3:+28804171 | MS.gene80404:CDS |
| GTTCCAGGACGGTAACGGTG+AGG | 0.680687 | 6.3:-28803997 | None:intergenic |
| GAAGTGCAACGGTTCCAGGA+CGG | 0.701371 | 6.3:-28804008 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAGGTTATTTAGAAAAAAAT+AGG | + | chr6.3:28804296-28804315 | MS.gene80404:intron | 15.0% |
| !!! | AATTTTGGATTCATAATTTA+GGG | + | chr6.3:28804081-28804100 | MS.gene80404:intron | 15.0% |
| !! | GTTGAGTTTATTCAAAAATT+AGG | + | chr6.3:28804199-28804218 | MS.gene80404:CDS | 20.0% |
| !! | TAGAAAAAAATAGGATTTCT+AGG | + | chr6.3:28804305-28804324 | MS.gene80404:CDS | 20.0% |
| !! | TCTGAAAACCTAAAAAATTA+GGG | - | chr6.3:28804049-28804068 | None:intergenic | 20.0% |
| !!! | AAAAACATAATTCCTGTTTT+AGG | + | chr6.3:28804277-28804296 | MS.gene80404:intron | 20.0% |
| !!! | AAAAATATCCTGAACTTTTA+GGG | + | chr6.3:28804458-28804477 | MS.gene80404:CDS | 20.0% |
| !!! | AGAGTTTTGAATTTGAATTT+TGG | + | chr6.3:28804066-28804085 | MS.gene80404:intron | 20.0% |
| !!! | ATACTGATTTATATCATCAA+TGG | - | chr6.3:28804230-28804249 | None:intergenic | 20.0% |
| !!! | GAATTTTGGATTCATAATTT+AGG | + | chr6.3:28804080-28804099 | MS.gene80404:intron | 20.0% |
| !!! | TTTTCTAAATAACCTAAAAC+AGG | - | chr6.3:28804292-28804311 | None:intergenic | 20.0% |
| ! | ATGTAGAGAAAGATGATATT+GGG | + | chr6.3:28804425-28804444 | MS.gene80404:CDS | 25.0% |
| ! | CTGAAAACCTAAAAAATTAG+GGG | - | chr6.3:28804048-28804067 | None:intergenic | 25.0% |
| ! | TCCTGTTATTAAGTTTCTTA+GGG | + | chr6.3:28804396-28804415 | MS.gene80404:CDS | 25.0% |
| ! | TGATTACTTGGTTATTTACT+TGG | + | chr6.3:28804621-28804640 | MS.gene80404:CDS | 25.0% |
| !! | CAAAAATATCCTGAACTTTT+AGG | + | chr6.3:28804457-28804476 | MS.gene80404:CDS | 25.0% |
| !! | CTCTGAAAACCTAAAAAATT+AGG | - | chr6.3:28804050-28804069 | None:intergenic | 25.0% |
| !!! | ACACAGTATCCTTATTTTTT+AGG | + | chr6.3:28804565-28804584 | MS.gene80404:CDS | 25.0% |
| !!! | CACAGTATCCTTATTTTTTA+GGG | + | chr6.3:28804566-28804585 | MS.gene80404:CDS | 25.0% |
| AAATCAGTATCCTTTGATTC+TGG | + | chr6.3:28804240-28804259 | MS.gene80404:intron | 30.0% | |
| AATCAGTATCCTTTGATTCT+GGG | + | chr6.3:28804241-28804260 | MS.gene80404:intron | 30.0% | |
| ACTCTCATCCCTAAAAAATA+AGG | - | chr6.3:28804577-28804596 | None:intergenic | 30.0% | |
| ATCATCTTCGATTATCTCAA+GGG | + | chr6.3:28804106-28804125 | MS.gene80404:CDS | 30.0% | |
| CCTAAAAAATTAGGGGAAAA+CGG | - | chr6.3:28804041-28804060 | None:intergenic | 30.0% | |
| CCTGTTATTAAGTTTCTTAG+GGG | + | chr6.3:28804397-28804416 | MS.gene80404:CDS | 30.0% | |
| CTCCTGTTATTAAGTTTCTT+AGG | + | chr6.3:28804395-28804414 | MS.gene80404:CDS | 30.0% | |
| GATGTAGAGAAAGATGATAT+TGG | + | chr6.3:28804424-28804443 | MS.gene80404:CDS | 30.0% | |
| TATCATCTTCGATTATCTCA+AGG | + | chr6.3:28804105-28804124 | MS.gene80404:CDS | 30.0% | |
| ! | AAAGCTTCTTGTTGATTACT+TGG | + | chr6.3:28804609-28804628 | MS.gene80404:CDS | 30.0% |
| !! | TAGTATTGGTGTTAATCCTA+GGG | + | chr6.3:28804525-28804544 | MS.gene80404:CDS | 30.0% |
| !! | TTAGTATTGGTGTTAATCCT+AGG | + | chr6.3:28804524-28804543 | MS.gene80404:CDS | 30.0% |
| !!! | TATTTTTTAGGGATGAGAGT+TGG | + | chr6.3:28804577-28804596 | MS.gene80404:CDS | 30.0% |
| AATTATCCCTGATGAATTGC+AGG | + | chr6.3:28804135-28804154 | MS.gene80404:CDS | 35.0% | |
| AGCTTAAACCCTAAAAGTTC+AGG | - | chr6.3:28804469-28804488 | None:intergenic | 35.0% | |
| CCCCTAAGAAACTTAATAAC+AGG | - | chr6.3:28804400-28804419 | None:intergenic | 35.0% | |
| GTGGCTTATCTTGTTAGTAT+TGG | + | chr6.3:28804511-28804530 | MS.gene80404:CDS | 35.0% | |
| TAAGGATACTGTGTAACCAT+AGG | - | chr6.3:28804559-28804578 | None:intergenic | 35.0% | |
| TTCGATTATCTCAAGGGTTT+GGG | + | chr6.3:28804112-28804131 | MS.gene80404:CDS | 35.0% | |
| ! | AGGATTTCTAGGTCTAAACT+TGG | + | chr6.3:28804316-28804335 | MS.gene80404:CDS | 35.0% |
| ! | CCGTTTTCCCCTAATTTTTT+AGG | + | chr6.3:28804038-28804057 | MS.gene80404:intron | 35.0% |
| !!! | CTTTTAGGGTTTAAGCTTGA+AGG | + | chr6.3:28804472-28804491 | MS.gene80404:CDS | 35.0% |
| !!! | TTTCATCGCTTTTTTCGTTC+AGG | + | chr6.3:28803933-28803952 | MS.gene80404:intron | 35.0% |
| ACACTACAACCCAGAATCAA+AGG | - | chr6.3:28804253-28804272 | None:intergenic | 40.0% | |
| ACACTAGCATGCAAAACTTG+TGG | - | chr6.3:28804361-28804380 | None:intergenic | 40.0% | |
| AGGTACTATGAGTACTTCAG+TGG | + | chr6.3:28804492-28804511 | MS.gene80404:CDS | 40.0% | |
| CCAAATCCTGCAATTCATCA+GGG | - | chr6.3:28804144-28804163 | None:intergenic | 40.0% | |
| CTTCGATTATCTCAAGGGTT+TGG | + | chr6.3:28804111-28804130 | MS.gene80404:CDS | 40.0% | |
| GGTGTTAATCCTAGGGATAT+TGG | + | chr6.3:28804532-28804551 | MS.gene80404:CDS | 40.0% | |
| TCCAAATCCTGCAATTCATC+AGG | - | chr6.3:28804145-28804164 | None:intergenic | 40.0% | |
| !! | TTTTTGACTCCTCCAGTAGT+AGG | - | chr6.3:28803976-28803995 | None:intergenic | 40.0% |
| !!! | CTTTTTTCGTTCAGGCTTCT+AGG | + | chr6.3:28803941-28803960 | MS.gene80404:intron | 40.0% |
| AAATCTGCTCCTACTACTGG+AGG | + | chr6.3:28803964-28803983 | MS.gene80404:CDS | 45.0% | |
| ACCATAGGACCAATATCCCT+AGG | - | chr6.3:28804544-28804563 | None:intergenic | 45.0% | |
| ACGGAATTACCGAAGTGCAA+CGG | - | chr6.3:28804022-28804041 | None:intergenic | 45.0% | |
| ACGTTCCCTCATGACTTCAA+CGG | - | chr6.3:28804179-28804198 | None:intergenic | 45.0% | |
| AGGAAATCTGCTCCTACTAC+TGG | + | chr6.3:28803961-28803980 | MS.gene80404:intron | 45.0% | |
| CCCTGATGAATTGCAGGATT+TGG | + | chr6.3:28804141-28804160 | MS.gene80404:CDS | 45.0% | |
| TCCTAGGGATATTGGTCCTA+TGG | + | chr6.3:28804540-28804559 | MS.gene80404:CDS | 45.0% | |
| TGCAACCGTTGAAGTCATGA+GGG | + | chr6.3:28804171-28804190 | MS.gene80404:CDS | 45.0% | |
| CTCATGACTTCAACGGTTGC+AGG | - | chr6.3:28804172-28804191 | None:intergenic | 50.0% | |
| CTGCAACCGTTGAAGTCATG+AGG | + | chr6.3:28804170-28804189 | MS.gene80404:CDS | 50.0% | |
| AAACCTCACCGTTACCGTCC+TGG | + | chr6.3:28803994-28804013 | MS.gene80404:CDS | 55.0% | |
| CAACGGTTCCAGGACGGTAA+CGG | - | chr6.3:28804005-28804024 | None:intergenic | 55.0% | |
| GAAGTGCAACGGTTCCAGGA+CGG | - | chr6.3:28804011-28804030 | None:intergenic | 55.0% | |
| GTCCTGGAACCGTTGCACTT+CGG | + | chr6.3:28804010-28804029 | MS.gene80404:CDS | 55.0% | |
| TACCGAAGTGCAACGGTTCC+AGG | - | chr6.3:28804015-28804034 | None:intergenic | 55.0% | |
| ! | GTTCCAGGACGGTAACGGTG+AGG | - | chr6.3:28804000-28804019 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 28803891 | 28804666 | 28803891 | ID=MS.gene80404 |
| chr6.3 | mRNA | 28803891 | 28804666 | 28803891 | ID=MS.gene80404.t1;Parent=MS.gene80404 |
| chr6.3 | exon | 28803891 | 28803898 | 28803891 | ID=MS.gene80404.t1.exon1;Parent=MS.gene80404.t1 |
| chr6.3 | CDS | 28803891 | 28803898 | 28803891 | ID=cds.MS.gene80404.t1;Parent=MS.gene80404.t1 |
| chr6.3 | exon | 28803963 | 28804031 | 28803963 | ID=MS.gene80404.t1.exon2;Parent=MS.gene80404.t1 |
| chr6.3 | CDS | 28803963 | 28804031 | 28803963 | ID=cds.MS.gene80404.t1;Parent=MS.gene80404.t1 |
| chr6.3 | exon | 28804102 | 28804220 | 28804102 | ID=MS.gene80404.t1.exon3;Parent=MS.gene80404.t1 |
| chr6.3 | CDS | 28804102 | 28804220 | 28804102 | ID=cds.MS.gene80404.t1;Parent=MS.gene80404.t1 |
| chr6.3 | exon | 28804299 | 28804666 | 28804299 | ID=MS.gene80404.t1.exon4;Parent=MS.gene80404.t1 |
| chr6.3 | CDS | 28804299 | 28804666 | 28804299 | ID=cds.MS.gene80404.t1;Parent=MS.gene80404.t1 |
| Gene Sequence |
| Protein sequence |
