AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80415

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80415.t1 MTR_3g465280 66.667 75 21 3 27 100 9 80 1.07e-24 99.8
MS.gene80415.t1 MTR_3g465280 42.149 121 60 5 4 122 81 193 2.12e-17 80.1
MS.gene80415.t1 MTR_4g079500 44.211 95 53 0 113 207 194 288 2.55e-18 83.2
MS.gene80415.t1 MTR_2g090900 43.158 95 54 0 113 207 191 285 2.04e-17 80.9
MS.gene80415.t1 MTR_2g090900 43.158 95 54 0 113 207 191 285 3.04e-17 80.1
MS.gene80415.t1 MTR_6g034945 35.088 114 74 0 112 225 150 263 1.99e-11 63.2
MS.gene80415.t1 MTR_5g075390 33.071 127 77 1 87 205 125 251 2.74e-11 63.2
MS.gene80415.t1 MTR_3g467120 38.298 94 58 0 112 205 176 269 4.57e-11 62.4
MS.gene80415.t1 MTR_4g072090 35.922 103 66 0 113 215 360 462 9.91e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80415.t1 AT2G42380 41.071 112 66 0 96 207 169 280 2.09e-19 85.9
MS.gene80415.t1 AT3G58120 40.708 113 66 1 95 207 185 296 1.26e-18 84.0
MS.gene80415.t1 AT2G42380 42.708 96 55 0 96 191 169 264 5.85e-17 78.6
MS.gene80415.t1 AT3G58120 42.268 97 55 1 95 191 185 280 3.57e-16 76.6
MS.gene80415.t1 AT2G42380 35.714 112 61 1 96 207 169 269 1.74e-13 69.3
MS.gene80415.t1 AT5G07160 40.860 93 55 0 113 205 48 140 5.70e-12 62.4
MS.gene80415.t1 AT2G40620 38.298 94 58 0 112 205 147 240 6.06e-11 62.0
MS.gene80415.t1 AT1G43700 39.362 94 57 0 112 205 193 286 8.43e-11 61.6

Find 51 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTTAACCAGCTGAAAT+TGG 0.206191 6.3:+29352026 MS.gene80415:intron
CAGCTATTTCAAGTCTTAAT+TGG 0.226858 6.3:-29350890 None:intergenic
GCTCGATTATATTGCTGATT+TGG 0.235809 6.3:+29350232 MS.gene80415:CDS
GCATGACCAAGATTTGATTT+TGG 0.261059 6.3:-29349586 None:intergenic
TTTGTACCAGTCATTCTCTT+TGG 0.270280 6.3:-29349373 None:intergenic
CTAACCATGTTTGATTTCTT+AGG 0.277873 6.3:-29349424 None:intergenic
GTATACCGTTTGAGCCTCTT+TGG 0.278573 6.3:-29349685 None:intergenic
TTCTCCATATTATGCTCATT+TGG 0.286048 6.3:-29352098 None:intergenic
CAGACCAAATGAGCATAATA+TGG 0.328196 6.3:+29352094 MS.gene80415:CDS
TTGTACCAGTCATTCTCTTT+GGG 0.334410 6.3:-29349372 None:intergenic
TTATGCTCATTTGGTCTGAT+TGG 0.342932 6.3:-29352089 None:intergenic
ATAAGAGAGCTGAGGTGAAT+AGG 0.348904 6.3:+29351044 MS.gene80415:CDS
TTCGATCATGGCAACCCTCT+TGG 0.403712 6.3:+29349476 MS.gene80415:CDS
ATGGTTAGACCTTCCTCATA+TGG 0.444092 6.3:+29349439 MS.gene80415:CDS
AGTGTACCAAAATCAAATCT+TGG 0.459311 6.3:+29349580 MS.gene80415:CDS
CGTCATCAACATTTCGATCA+TGG 0.470936 6.3:+29349464 MS.gene80415:CDS
TATTGCTGATTTGGAGCGGA+TGG 0.492832 6.3:+29350241 MS.gene80415:CDS
TCTTAATTGGTGGTGTTCCA+TGG 0.506174 6.3:-29350877 None:intergenic
CTATTTCAAGTCTTAATTGG+TGG 0.506362 6.3:-29350887 None:intergenic
TGGATCCAAAGAGGCTCAAA+CGG 0.511736 6.3:+29349680 MS.gene80415:CDS
AAACAAGAGGCGAGTAGCAG+TGG 0.525757 6.3:+29349517 MS.gene80415:CDS
GAGCTGAGGTGAATAGGCTG+AGG 0.526514 6.3:+29351050 MS.gene80415:CDS
GGCATCCCAAAGAGAATGAC+TGG 0.538746 6.3:+29349367 MS.gene80415:CDS
GTGCAGAAATTCGATTTGCT+AGG 0.542578 6.3:-29350190 None:intergenic
AACATGTTGCACACAGAAGA+GGG 0.550817 6.3:+29349394 MS.gene80415:CDS
TTGCACACAGAAGAGGGACC+TGG 0.552875 6.3:+29349400 MS.gene80415:CDS
TGTTGATGACGATCCATATG+AGG 0.557119 6.3:-29349452 None:intergenic
ATGTTTGATTTCTTAGGCCC+AGG 0.557475 6.3:-29349418 None:intergenic
AGACTTCAACTTGTGGGCGA+AGG 0.559195 6.3:-29350830 None:intergenic
GATTGAGAATAAGAGAGCTG+AGG 0.559603 6.3:+29351036 MS.gene80415:CDS
GCATCTGCATGTGTTGGTGT+TGG 0.564639 6.3:+29349637 MS.gene80415:CDS
ATGCACTACCGCCACTGATG+TGG 0.592583 6.3:-29349618 None:intergenic
TGCACACAGAAGAGGGACCT+GGG 0.596481 6.3:+29349401 MS.gene80415:CDS
TTGCTGAAGCACAAAACAAG+AGG 0.602002 6.3:+29351092 MS.gene80415:CDS
AAACATGTTGCACACAGAAG+AGG 0.606246 6.3:+29349393 MS.gene80415:CDS
GGTAGTGCATCTGCATGTGT+TGG 0.606657 6.3:+29349631 MS.gene80415:CDS
TCAGCAGTGGTTGTTGACCA+TGG 0.606900 6.3:+29350860 MS.gene80415:CDS
AGCTGAGGTGAATAGGCTGA+GGG 0.614102 6.3:+29351051 MS.gene80415:CDS
ATTATATTGCTGATTTGGAG+CGG 0.619255 6.3:+29350237 MS.gene80415:CDS
TTAACCAGCTGAAATTGGAG+AGG 0.622671 6.3:+29352031 MS.gene80415:intron
AAAGAAAGGATACTTCAAGA+AGG 0.624481 6.3:+29350921 MS.gene80415:CDS
AGGTCAATGCTCTTCCAAGA+GGG 0.634364 6.3:-29349490 None:intergenic
TACTCGCCTCTTGTTTACCG+AGG 0.637734 6.3:-29349510 None:intergenic
CATTGACCTCGGTAAACAAG+AGG 0.644199 6.3:+29349504 MS.gene80415:CDS
GCTGAGAAGAACCACATCAG+TGG 0.645935 6.3:+29349607 MS.gene80415:CDS
GATGACGATCCATATGAGGA+AGG 0.651960 6.3:-29349448 None:intergenic
GAGGTCAATGCTCTTCCAAG+AGG 0.660030 6.3:-29349491 None:intergenic
GAGAAGAACCACATCAGTGG+CGG 0.665888 6.3:+29349610 MS.gene80415:CDS
AAATTGGAGAGGAAGCGTCA+AGG 0.673271 6.3:+29352042 MS.gene80415:CDS
TCTTGGAAGAGCATTGACCT+CGG 0.690835 6.3:+29349493 MS.gene80415:CDS
TGGGCCTAAGAAATCAAACA+TGG 0.705886 6.3:+29349420 MS.gene80415:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATAAATCTATATGT+TGG + chr6.3:29349964-29349983 MS.gene80415:intron 10.0%
!! CTGAAAAAAAAATTAATAAT+GGG + chr6.3:29349847-29349866 MS.gene80415:intron 10.0%
!! TAATATAGAAAAATATGTTA+TGG - chr6.3:29351820-29351839 None:intergenic 10.0%
!! AAAATCATTGTAAACAATAT+CGG - chr6.3:29351617-29351636 None:intergenic 15.0%
!! AAAATTGAAACAAAATATCT+CGG + chr6.3:29350094-29350113 MS.gene80415:intron 15.0%
!! GCTGAAAAAAAAATTAATAA+TGG + chr6.3:29349846-29349865 MS.gene80415:intron 15.0%
!!! AAAAAAAAATTAATAATGGG+AGG + chr6.3:29349850-29349869 MS.gene80415:intron 15.0%
!!! ATTTTTTTTTTTGTATATGC+AGG + chr6.3:29350794-29350813 MS.gene80415:intron 15.0%
!!! GATTTTTTTCAATTTCAATT+TGG - chr6.3:29350438-29350457 None:intergenic 15.0%
!! ACATACTAATGTGAAATAAA+TGG - chr6.3:29351002-29351021 None:intergenic 20.0%
!! ACTCATACATGATAAAAATA+TGG + chr6.3:29351236-29351255 MS.gene80415:intron 20.0%
!! GTCGATTATTTGTATTAATT+TGG + chr6.3:29350537-29350556 MS.gene80415:intron 20.0%
!! GTTGTTTATTATAGATCAAA+CGG - chr6.3:29351659-29351678 None:intergenic 20.0%
!! TGTTTATCAGAAAAAATGAA+GGG + chr6.3:29349346-29349365 MS.gene80415:intron 20.0%
!! TTGTTTATCAGAAAAAATGA+AGG + chr6.3:29349345-29349364 MS.gene80415:intron 20.0%
!!! ACTTCTATCATGTTTTTTTA+GGG + chr6.3:29351872-29351891 MS.gene80415:intron 20.0%
!!! ATATTATATCGATTATGTGT+AGG - chr6.3:29350130-29350149 None:intergenic 20.0%
!!! CAAATTCGTTTTTTGTTTTA+AGG - chr6.3:29351458-29351477 None:intergenic 20.0%
!!! TACTTCTATCATGTTTTTTT+AGG + chr6.3:29351871-29351890 MS.gene80415:intron 20.0%
!!! TTATTAGTTTTTTTGTCTTG+TGG - chr6.3:29349556-29349575 None:intergenic 20.0%
!!! TTTTTCTGATAAACAAGAAA+TGG - chr6.3:29349341-29349360 None:intergenic 20.0%
! ATAGATCGATTATGATTGAA+TGG - chr6.3:29350598-29350617 None:intergenic 25.0%
! ATGAATATGTAAGTAGGTAT+GGG - chr6.3:29351769-29351788 None:intergenic 25.0%
! ATTAGTATGTTTATTTCCAG+TGG + chr6.3:29351012-29351031 MS.gene80415:intron 25.0%
! CAGTTGGCAAATTAAAAATA+TGG + chr6.3:29349745-29349764 MS.gene80415:intron 25.0%
! CATAACACTAAAAGTTTGAT+GGG + chr6.3:29349787-29349806 MS.gene80415:intron 25.0%
! GTCAATATGAATATGTAAGT+AGG - chr6.3:29351775-29351794 None:intergenic 25.0%
! TAGTATACTGATATATGTTG+CGG + chr6.3:29351482-29351501 MS.gene80415:intron 25.0%
! TATGAATATGTAAGTAGGTA+TGG - chr6.3:29351770-29351789 None:intergenic 25.0%
! TCATAACACTAAAAGTTTGA+TGG + chr6.3:29349786-29349805 MS.gene80415:intron 25.0%
! TCATGTATCAACTTGTAATT+TGG + chr6.3:29350618-29350637 MS.gene80415:intron 25.0%
! TCTTGATCTGATTATTTGAA+TGG + chr6.3:29350676-29350695 MS.gene80415:intron 25.0%
! TGACATTCAATTAACATAGT+GGG + chr6.3:29351791-29351810 MS.gene80415:intron 25.0%
! TTGACATTCAATTAACATAG+TGG + chr6.3:29351790-29351809 MS.gene80415:intron 25.0%
!! CAAATTGAAATTCTTTGAAG+TGG - chr6.3:29350518-29350537 None:intergenic 25.0%
!!! ATATGTTGGTTTAAGTTTCA+TGG + chr6.3:29349978-29349997 MS.gene80415:intron 25.0%
!!! TCTTCACAAGTTTTTTGTTT+GGG - chr6.3:29350762-29350781 None:intergenic 25.0%
AAAGAAAGGATACTTCAAGA+AGG + chr6.3:29350921-29350940 MS.gene80415:CDS 30.0%
AAATTCAGATCCAAAAAGCA+GGG - chr6.3:29351536-29351555 None:intergenic 30.0%
AATTCTGTGATTTATTCCAG+CGG + chr6.3:29351700-29351719 MS.gene80415:intron 30.0%
AGATCAAACGGTTCAAATTA+AGG - chr6.3:29351647-29351666 None:intergenic 30.0%
AGTGTACCAAAATCAAATCT+TGG + chr6.3:29349580-29349599 MS.gene80415:CDS 30.0%
AGTTTGATGGGAATAGTATT+TGG + chr6.3:29349799-29349818 MS.gene80415:intron 30.0%
CAAATCAACTATATTAGCCA+GGG - chr6.3:29350039-29350058 None:intergenic 30.0%
GAATTTCCTATTGTTAGAAC+AGG + chr6.3:29351550-29351569 MS.gene80415:intron 30.0%
GATCAAACGGTTCAAATTAA+GGG - chr6.3:29351646-29351665 None:intergenic 30.0%
TATAATTATATGTGTGCTCG+CGG + chr6.3:29351395-29351414 MS.gene80415:intron 30.0%
TCAAATCAACTATATTAGCC+AGG - chr6.3:29350040-29350059 None:intergenic 30.0%
TGAATATGTAAGTAGGTATG+GGG - chr6.3:29351768-29351787 None:intergenic 30.0%
TGTTTGATCCAAAACAACAA+GGG + chr6.3:29350714-29350733 MS.gene80415:intron 30.0%
TTCTCCATATTATGCTCATT+TGG - chr6.3:29352101-29352120 None:intergenic 30.0%
TTGTTTGATCCAAAACAACA+AGG + chr6.3:29350713-29350732 MS.gene80415:intron 30.0%
TTTCTTTAACCAGCTGAAAT+TGG + chr6.3:29352026-29352045 MS.gene80415:intron 30.0%
! AAAGTGAGAGTATAGATAGA+AGG + chr6.3:29351313-29351332 MS.gene80415:intron 30.0%
! CAGCTATTTCAAGTCTTAAT+TGG - chr6.3:29350893-29350912 None:intergenic 30.0%
! CTAACCATGTTTGATTTCTT+AGG - chr6.3:29349427-29349446 None:intergenic 30.0%
! CTATTTCAAGTCTTAATTGG+TGG - chr6.3:29350890-29350909 None:intergenic 30.0%
! TTTTGTAGACTTCAACTTGT+GGG - chr6.3:29350839-29350858 None:intergenic 30.0%
! TTTTGTCTTCTGTTGATCTT+AGG + chr6.3:29350165-29350184 MS.gene80415:intron 30.0%
! TTTTTGTAGACTTCAACTTG+TGG - chr6.3:29350840-29350859 None:intergenic 30.0%
!! ATTATATTGCTGATTTGGAG+CGG + chr6.3:29350237-29350256 MS.gene80415:CDS 30.0%
!!! AATGGGAGGTTGTTTTATTT+AGG + chr6.3:29349864-29349883 MS.gene80415:intron 30.0%
!!! CTCTTCACAAGTTTTTTGTT+TGG - chr6.3:29350763-29350782 None:intergenic 30.0%
!!! GTTTTTTGTTTGGGTAGATA+TGG - chr6.3:29350753-29350772 None:intergenic 30.0%
!!! TGTTTTATTTAGGCTTGTCA+TGG + chr6.3:29349874-29349893 MS.gene80415:intron 30.0%
AAGTCTACAAAAATCAGCAG+TGG + chr6.3:29350847-29350866 MS.gene80415:CDS 35.0%
AATTCAGATCCAAAAAGCAG+GGG - chr6.3:29351535-29351554 None:intergenic 35.0%
ACCTTCAGATCTTAAATGTG+TGG + chr6.3:29351591-29351610 MS.gene80415:intron 35.0%
ATTCTGTGATTTATTCCAGC+GGG + chr6.3:29351701-29351720 MS.gene80415:intron 35.0%
CAGACCAAATGAGCATAATA+TGG + chr6.3:29352094-29352113 MS.gene80415:CDS 35.0%
CATCTATTAATGTCATACCC+TGG + chr6.3:29350019-29350038 MS.gene80415:intron 35.0%
CATTCTCATCAAAAGACCAT+AGG - chr6.3:29350392-29350411 None:intergenic 35.0%
GAAAATACACGCAAACACAA+AGG - chr6.3:29351266-29351285 None:intergenic 35.0%
GAAATTCAGATCCAAAAAGC+AGG - chr6.3:29351537-29351556 None:intergenic 35.0%
GAATATGTAAGTAGGTATGG+GGG - chr6.3:29351767-29351786 None:intergenic 35.0%
GAGAAAATCACTAAGGAAGA+GGG - chr6.3:29351745-29351764 None:intergenic 35.0%
TAGAAGTAGTGAAGCAGATA+CGG - chr6.3:29351859-29351878 None:intergenic 35.0%
TAGCTGTACGAGAAAAAGAA+AGG + chr6.3:29350907-29350926 MS.gene80415:CDS 35.0%
TGAGCAACACAAAAATCAGT+TGG + chr6.3:29349729-29349748 MS.gene80415:intron 35.0%
TGATCAAGCAACACAAAAAC+TGG + chr6.3:29349660-29349679 MS.gene80415:CDS 35.0%
TTATGCTCATTTGGTCTGAT+TGG - chr6.3:29352092-29352111 None:intergenic 35.0%
TTGTACCAGTCATTCTCTTT+GGG - chr6.3:29349375-29349394 None:intergenic 35.0%
TTTGTACCAGTCATTCTCTT+TGG - chr6.3:29349376-29349395 None:intergenic 35.0%
TTTGTTTGGGTAGATATGGT+GGG - chr6.3:29350749-29350768 None:intergenic 35.0%
! GCTCGATTATATTGCTGATT+TGG + chr6.3:29350232-29350251 MS.gene80415:CDS 35.0%
!! GCATGACCAAGATTTGATTT+TGG - chr6.3:29349589-29349608 None:intergenic 35.0%
!! TTTTGTTTGGGTAGATATGG+TGG - chr6.3:29350750-29350769 None:intergenic 35.0%
!!! ATATGGATCCCTTGTTGTTT+TGG - chr6.3:29350725-29350744 None:intergenic 35.0%
!!! TTTCAAGTTGTTTTGCGTCT+TGG - chr6.3:29351681-29351700 None:intergenic 35.0%
AAACATGTTGCACACAGAAG+AGG + chr6.3:29349393-29349412 MS.gene80415:CDS 40.0%
AACATGTTGCACACAGAAGA+GGG + chr6.3:29349394-29349413 MS.gene80415:CDS 40.0%
ACTGATATATGTTGCGGTTG+TGG + chr6.3:29351488-29351507 MS.gene80415:intron 40.0%
ATAAGAGAGCTGAGGTGAAT+AGG + chr6.3:29351044-29351063 MS.gene80415:CDS 40.0%
ATGGTTAGACCTTCCTCATA+TGG + chr6.3:29349439-29349458 MS.gene80415:CDS 40.0%
CACAAAAACTGGATCCAAAG+AGG + chr6.3:29349671-29349690 MS.gene80415:CDS 40.0%
CGTCATCAACATTTCGATCA+TGG + chr6.3:29349464-29349483 MS.gene80415:CDS 40.0%
CTCTTATTCTCAATCTCCAC+TGG - chr6.3:29351031-29351050 None:intergenic 40.0%
GATTGAGAATAAGAGAGCTG+AGG + chr6.3:29351036-29351055 MS.gene80415:CDS 40.0%
GCGGATGGAAAAATATTTGC+AGG + chr6.3:29350256-29350275 MS.gene80415:CDS 40.0%
GGAGAAAATCACTAAGGAAG+AGG - chr6.3:29351746-29351765 None:intergenic 40.0%
TATGGGGGAGAAAATCACTA+AGG - chr6.3:29351752-29351771 None:intergenic 40.0%
TGGGCCTAAGAAATCAAACA+TGG + chr6.3:29349420-29349439 MS.gene80415:CDS 40.0%
TTAACCAGCTGAAATTGGAG+AGG + chr6.3:29352031-29352050 MS.gene80415:intron 40.0%
TTGCTGAAGCACAAAACAAG+AGG + chr6.3:29351092-29351111 MS.gene80415:CDS 40.0%
! ATGTTTGATTTCTTAGGCCC+AGG - chr6.3:29349421-29349440 None:intergenic 40.0%
! GCCACACATTTAAGATCTGA+AGG - chr6.3:29351595-29351614 None:intergenic 40.0%
! GTGCAGAAATTCGATTTGCT+AGG - chr6.3:29350193-29350212 None:intergenic 40.0%
!! TCTTAATTGGTGGTGTTCCA+TGG - chr6.3:29350880-29350899 None:intergenic 40.0%
!! TGTTGATGACGATCCATATG+AGG - chr6.3:29349455-29349474 None:intergenic 40.0%
!! TTCTTTGAAGTGGTAACCAG+CGG - chr6.3:29350508-29350527 None:intergenic 40.0%
AGACCATAGGCTTTCACGAT+CGG - chr6.3:29350379-29350398 None:intergenic 45.0%
AGGTCAATGCTCTTCCAAGA+GGG - chr6.3:29349493-29349512 None:intergenic 45.0%
CAGCGTCCTGTTCTAACAAT+AGG - chr6.3:29351559-29351578 None:intergenic 45.0%
CATTGACCTCGGTAAACAAG+AGG + chr6.3:29349504-29349523 MS.gene80415:CDS 45.0%
GATGACGATCCATATGAGGA+AGG - chr6.3:29349451-29349470 None:intergenic 45.0%
GTATACCGTTTGAGCCTCTT+TGG - chr6.3:29349688-29349707 None:intergenic 45.0%
TGGATCCAAAGAGGCTCAAA+CGG + chr6.3:29349680-29349699 MS.gene80415:CDS 45.0%
TTTATTCCAGCGGGCAATCT+TGG + chr6.3:29351710-29351729 MS.gene80415:intron 45.0%
! GGGTAGATATGGTGGGAATA+TGG - chr6.3:29350742-29350761 None:intergenic 45.0%
!! AAATTGGAGAGGAAGCGTCA+AGG + chr6.3:29352042-29352061 MS.gene80415:CDS 45.0%
!! TATTGCTGATTTGGAGCGGA+TGG + chr6.3:29350241-29350260 MS.gene80415:CDS 45.0%
!! TCTTGGAAGAGCATTGACCT+CGG + chr6.3:29349493-29349512 MS.gene80415:CDS 45.0%
!!! TTTTTCAAAATTTATAATTA+GGG + chr6.3:29351187-29351206 MS.gene80415:intron 5.0%
!!! TTTTTTCAAAATTTATAATT+AGG + chr6.3:29351186-29351205 MS.gene80415:intron 5.0%
AAACAAGAGGCGAGTAGCAG+TGG + chr6.3:29349517-29349536 MS.gene80415:CDS 50.0%
ACCATAGGCTTTCACGATCG+GGG - chr6.3:29350377-29350396 None:intergenic 50.0%
AGACTTCAACTTGTGGGCGA+AGG - chr6.3:29350833-29350852 None:intergenic 50.0%
AGCTGAGGTGAATAGGCTGA+GGG + chr6.3:29351051-29351070 MS.gene80415:CDS 50.0%
ATGTTGCGGTTGTGGCATGT+GGG + chr6.3:29351496-29351515 MS.gene80415:intron 50.0%
GACCATAGGCTTTCACGATC+GGG - chr6.3:29350378-29350397 None:intergenic 50.0%
GAGAAGAACCACATCAGTGG+CGG + chr6.3:29349610-29349629 MS.gene80415:CDS 50.0%
GAGGTCAATGCTCTTCCAAG+AGG - chr6.3:29349494-29349513 None:intergenic 50.0%
GCTGAGAAGAACCACATCAG+TGG + chr6.3:29349607-29349626 MS.gene80415:CDS 50.0%
GCTTCCTCTCCAATTTCAGC+TGG - chr6.3:29352038-29352057 None:intergenic 50.0%
GGCATCCCAAAGAGAATGAC+TGG + chr6.3:29349367-29349386 MS.gene80415:CDS 50.0%
GGTAGTGCATCTGCATGTGT+TGG + chr6.3:29349631-29349650 MS.gene80415:CDS 50.0%
TATGTTGCGGTTGTGGCATG+TGG + chr6.3:29351495-29351514 MS.gene80415:intron 50.0%
TCAGCAGTGGTTGTTGACCA+TGG + chr6.3:29350860-29350879 MS.gene80415:CDS 50.0%
TTCGATCATGGCAACCCTCT+TGG + chr6.3:29349476-29349495 MS.gene80415:CDS 50.0%
! GCATCTGCATGTGTTGGTGT+TGG + chr6.3:29349637-29349656 MS.gene80415:CDS 50.0%
! TACTCGCCTCTTGTTTACCG+AGG - chr6.3:29349513-29349532 None:intergenic 50.0%
!! GGTTTGTAGCCCCTGCTTTT+TGG + chr6.3:29351523-29351542 MS.gene80415:intron 50.0%
ATGCACTACCGCCACTGATG+TGG - chr6.3:29349621-29349640 None:intergenic 55.0%
CAAGAACCAAGATTGCCCGC+TGG - chr6.3:29351719-29351738 None:intergenic 55.0%
GAGCTGAGGTGAATAGGCTG+AGG + chr6.3:29351050-29351069 MS.gene80415:CDS 55.0%
TCCCCGATCGTGAAAGCCTA+TGG + chr6.3:29350373-29350392 MS.gene80415:intron 55.0%
TGCACACAGAAGAGGGACCT+GGG + chr6.3:29349401-29349420 MS.gene80415:CDS 55.0%
TGCTCTACATGTAGAGCCGC+TGG + chr6.3:29350489-29350508 MS.gene80415:intron 55.0%
TTGCACACAGAAGAGGGACC+TGG + chr6.3:29349400-29349419 MS.gene80415:CDS 55.0%
CGGTTGTGGCATGTGGGTAG+GGG + chr6.3:29351502-29351521 MS.gene80415:intron 60.0%
GCGGTTGTGGCATGTGGGTA+GGG + chr6.3:29351501-29351520 MS.gene80415:intron 60.0%
TGCGGTTGTGGCATGTGGGT+AGG + chr6.3:29351500-29351519 MS.gene80415:intron 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 29349245 29352124 29349245 ID=MS.gene80415
chr6.3 mRNA 29349245 29352124 29349245 ID=MS.gene80415.t1;Parent=MS.gene80415
chr6.3 exon 29349245 29349254 29349245 ID=MS.gene80415.t1.exon1;Parent=MS.gene80415.t1
chr6.3 CDS 29349245 29349254 29349245 ID=cds.MS.gene80415.t1;Parent=MS.gene80415.t1
chr6.3 exon 29349356 29349701 29349356 ID=MS.gene80415.t1.exon2;Parent=MS.gene80415.t1
chr6.3 CDS 29349356 29349701 29349356 ID=cds.MS.gene80415.t1;Parent=MS.gene80415.t1
chr6.3 exon 29350187 29350277 29350187 ID=MS.gene80415.t1.exon3;Parent=MS.gene80415.t1
chr6.3 CDS 29350187 29350277 29350187 ID=cds.MS.gene80415.t1;Parent=MS.gene80415.t1
chr6.3 exon 29350816 29350942 29350816 ID=MS.gene80415.t1.exon4;Parent=MS.gene80415.t1
chr6.3 CDS 29350816 29350942 29350816 ID=cds.MS.gene80415.t1;Parent=MS.gene80415.t1
chr6.3 exon 29351032 29351133 29351032 ID=MS.gene80415.t1.exon5;Parent=MS.gene80415.t1
chr6.3 CDS 29351032 29351133 29351032 ID=cds.MS.gene80415.t1;Parent=MS.gene80415.t1
chr6.3 exon 29352039 29352124 29352039 ID=MS.gene80415.t1.exon6;Parent=MS.gene80415.t1
chr6.3 CDS 29352039 29352124 29352039 ID=cds.MS.gene80415.t1;Parent=MS.gene80415.t1
Gene Sequence

>MS.gene80415

ATGGAAAAAGGTTAGTAATTTTTTTTCTTCTTTCTATTAAGTTTGTGTGAATTTGAGTCTCTTATTTCAACATATTGAGATGATGCATGTTTTCCATTTCTTGTTTATCAGAAAAAATGAAGGGCATCCCAAAGAGAATGACTGGTACAAACATGTTGCACACAGAAGAGGGACCTGGGCCTAAGAAATCAAACATGGTTAGACCTTCCTCATATGGATCGTCATCAACATTTCGATCATGGCAACCCTCTTGGAAGAGCATTGACCTCGGTAAACAAGAGGCGAGTAGCAGTGGAGTCAAAGCAACTCCACAAGACAAAAAAACTAATAACATTAGTGTACCAAAATCAAATCTTGGTCATGCTGAGAAGAACCACATCAGTGGCGGTAGTGCATCTGCATGTGTTGGTGTTGGTGATCAAGCAACACAAAAACTGGATCCAAAGAGGCTCAAACGGTATACTTGTGTTACTTATTGTTGTTATGAGCAACACAAAAATCAGTTGGCAAATTAAAAATATGGAAGATCGAAATTTTTTATTCATAACACTAAAAGTTTGATGGGAATAGTATTTGGTTTTTATTACATTTTTTTGTGAAAGCTGAAAAAAAAATTAATAATGGGAGGTTGTTTTATTTAGGCTTGTCATGGTCAACTGTGATTTTAATAATAAATTTAATAAATTTTGTAATTGTTTTCATCTTATTTTTAAAAAAAAATTAAAATAAATCTATATGTTGGTTTAAGTTTCATGGTATTTAGATTAGATTTATCATCTATTAATGTCATACCCTGGCTAATATAGTTGATTTGATTGATTTTATTCTAATCAAATCTAAATCAGTGAAAAAATTGAAACAAAATATCTCGGTTGTTTATTTCCTACACATAATCGATATAATATATTTTTATTTTCATCTTTTGTCTTCTGTTGATCTTAGGATCCTAGCAAATCGAATTTCTGCACAAAAATCGAGATTGAAAAAGCTCGATTATATTGCTGATTTGGAGCGGATGGAAAAATATTTGCAGGTTCACATCATTATCTATCGTATCTCAAAGATTGTAATTTCTGAATTTTTTGTCTTATTAATATATGTTCACGTGTCTCGAAGAGAACTAGATTATCCCCGATCGTGAAAGCCTATGGTCTTTTGATGAGAATGAAACAACGCAAATTCTAATTTCACCAAATTGAAATTGAAAAAAATCATATAATTTGTGTTGTTTGATCTTCATCAATTGCTCTACATGTAGAGCCGCTGGTTACCACTTCAAAGAATTTCAATTTGTCGATTATTTGTATTAATTTGGTGTAGACAAATACACAACGATCTTGTTGATTTGAACCATTCAATCATAATCGATCTATTCATGTATCAACTTGTAATTTGGAATTCAATTGTAAATTTGACATGCGACTCATTTGATCTTGATCTGATTATTTGAATGGCATAACTCAACAAATTGTTTGATCCAAAACAACAAGGGATCCATATTCCCACCATATCTACCCAAACAAAAAACTTGTGAAGAGTTGATTTTGAGATTTTTTTTTTTGTATATGCAGGCTGAGTTATCTTTCCTTCGCCCACAAGTTGAAGTCTACAAAAATCAGCAGTGGTTGTTGACCATGGAACACCACCAATTAAGACTTGAAATAGCTGTACGAGAAAAAGAAAGGATACTTCAAGAAGGTGATAACACTCATTAGTCTTTATTTATATCTCTAGCATTTATTCTCATGTCTCTACCATTTATTTCACATTAGTATGTTTATTTCCAGTGGAGATTGAGAATAAGAGAGCTGAGGTGAATAGGCTGAGGGAGCTTCAGAAGAAACTTGTTGCTGAAGCACAAAACAAGAGGTATATTGAAATGCTTACTAGTATTTTTAACAATATTTTTGTACTAATAAAATGACTCATATGTCTAAATTGTTTTTTCAAAATTTATAATTAGGGTTTGTATATTAAAAATAATATATCATACTCATACATGATAAAAATATGGTACTCCTTTGTGTTTGCGTGTATTTTCATGATCTGTGCATGTTGTATAAGATAAAAAGTGAGAGTATAGATAGAAGGATACAATGTTACACTTACATAGATGTCTTAAAATTTGTATTTAAAAAATACAACTCAAATATAATTATATGTGTGCTCGCGGATGTGTGTGTATATACATGTATAATCTTTGTTGATATCCTTAAAACAAAAAACGAATTTGTTGATAGTATACTGATATATGTTGCGGTTGTGGCATGTGGGTAGGGGTTTGTAGCCCCTGCTTTTTGGATCTGAATTTCCTATTGTTAGAACAGGACGCTGTAACAGATCAAAACCTTCAGATCTTAAATGTGTGGCCGATATTGTTTACAATGATTTTATAACTCCCTTAATTTGAACCGTTTGATCTATAATAAACAACCAAGACGCAAAACAACTTGAAAATTCTGTGATTTATTCCAGCGGGCAATCTTGGTTCTTGTTTCCCTCTTCCTTAGTGATTTTCTCCCCCATACCTACTTACATATTCATATTGACATTCAATTAACATAGTGGGCAGCCATAACATATTTTTCTATATTATACATATCATGTTTGTCCGTATCTGCTTCACTACTTCTATCATGTTTTTTTAGGGATTGTATCATGTTTATCTGCATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAATACACACATATTTTTAACTATATTTTGCTAATTTCTTTAACCAGCTGAAATTGGAGAGGAAGCGTCAAGGATGATGCAAACTGTTACTACTATGCCAATCAGACCAAATGAGCATAATATGGAGAAATAA
Protein sequence

>MS.gene80415.t1

MEKEKMKGIPKRMTGTNMLHTEEGPGPKKSNMVRPSSYGSSSTFRSWQPSWKSIDLGKQEASSSGVKATPQDKKTNNISVPKSNLGHAEKNHISGGSASACVGVGDQATQKLDPKRLKRILANRISAQKSRLKKLDYIADLERMEKYLQAELSFLRPQVEVYKNQQWLLTMEHHQLRLEIAVREKERILQEVEIENKRAEVNRLRELQKKLVAEAQNKRYIEMLTTEIGEEASRMMQTVTTMPIRPNEHNMEK