Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80458.t1 | RHN49639.1 | 69.1 | 149 | 30 | 2 | 75 | 208 | 7 | 154 | 4.90E-49 | 204.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80458.t1 | A0A396H8N5 | 69.1 | 149 | 30 | 2 | 75 | 208 | 7 | 154 | 3.5e-49 | 204.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene80458.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80458.t1 | MTR_7g117590 | 66.667 | 150 | 35 | 2 | 73 | 208 | 11 | 159 | 4.01e-65 | 199 |
| MS.gene80458.t1 | MTR_1g054470 | 50.000 | 148 | 48 | 4 | 59 | 206 | 8 | 129 | 8.77e-41 | 136 |
| MS.gene80458.t1 | MTR_2g062330 | 40.385 | 104 | 57 | 3 | 106 | 208 | 27 | 126 | 1.26e-21 | 87.0 |
| MS.gene80458.t1 | MTR_2g012700 | 37.113 | 97 | 60 | 1 | 113 | 208 | 11 | 107 | 6.75e-20 | 82.0 |
| MS.gene80458.t1 | MTR_5g071400 | 67.273 | 55 | 16 | 2 | 73 | 127 | 7 | 59 | 4.92e-19 | 79.0 |
| MS.gene80458.t1 | MTR_5g071400 | 73.684 | 38 | 8 | 2 | 90 | 127 | 1 | 36 | 7.74e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGATCGGGTCATTATTT+TGG | 0.084758 | 6.3:+38311531 | MS.gene80458:CDS |
| GAAATGCTTGGGTTATTATA+AGG | 0.206478 | 6.3:+38311637 | MS.gene80458:CDS |
| TACCGGAAATGGGTGAAAAT+TGG | 0.219124 | 6.3:-38312089 | None:intergenic |
| CGAATCGTTTATGATGAAAA+TGG | 0.245135 | 6.3:+38312384 | MS.gene80458:CDS |
| ATAACCCAAGCATTTCTAAA+TGG | 0.252951 | 6.3:-38311630 | None:intergenic |
| CCATCAACTTCTTCTTCGTT+TGG | 0.276341 | 6.3:-38312121 | None:intergenic |
| ATGGACCGGGCAAACAATTT+CGG | 0.291715 | 6.3:+38311599 | MS.gene80458:CDS |
| TTTGAAACTGTGCAAATATT+GGG | 0.304159 | 6.3:-38312144 | None:intergenic |
| TGGACCGGGCAAACAATTTC+GGG | 0.354349 | 6.3:+38311600 | MS.gene80458:CDS |
| ATAAGGGTTGTATTGAATCT+TGG | 0.363489 | 6.3:+38311654 | MS.gene80458:CDS |
| CTTTGGATTGCAATCTTCTC+AGG | 0.365114 | 6.3:-38312336 | None:intergenic |
| GAATGTGAAGTTCGTGATAA+AGG | 0.381874 | 6.3:+38312417 | MS.gene80458:CDS |
| AAATGCTTGGGTTATTATAA+GGG | 0.385756 | 6.3:+38311638 | MS.gene80458:CDS |
| AAGTTCGTGATAAAGGAAAT+TGG | 0.388847 | 6.3:+38312424 | MS.gene80458:CDS |
| ATTGATTCACATACATACTT+TGG | 0.390585 | 6.3:-38312353 | None:intergenic |
| ACCGGAAATGGGTGAAAATT+GGG | 0.397872 | 6.3:-38312088 | None:intergenic |
| GGCTGTTGGAAGGAATGTTC+TGG | 0.414323 | 6.3:+38312219 | MS.gene80458:CDS |
| CATCCCATTTAGAAATGCTT+GGG | 0.433499 | 6.3:+38311626 | MS.gene80458:CDS |
| TAACCCAAGCATTTCTAAAT+GGG | 0.438694 | 6.3:-38311629 | None:intergenic |
| CCAAACGAAGAAGAAGTTGA+TGG | 0.464081 | 6.3:+38312121 | MS.gene80458:CDS |
| ATTAGTTCTGCTACGGCTGT+TGG | 0.466648 | 6.3:+38312205 | MS.gene80458:CDS |
| GCATCCCATTTAGAAATGCT+TGG | 0.467931 | 6.3:+38311625 | MS.gene80458:CDS |
| GAAGATGAAGTTAGTGAAAG+AGG | 0.471983 | 6.3:+38311500 | None:intergenic |
| GCGACCCGAAATTGTTTGCC+CGG | 0.504401 | 6.3:-38311604 | None:intergenic |
| TGAAAATGTCTTCCTCTAGC+AGG | 0.518806 | 6.3:+38311718 | MS.gene80458:CDS |
| TGAAGTTAGTGAAAGAGGAT+AGG | 0.524234 | 6.3:+38311505 | MS.gene80458:CDS |
| CTGCAGCGATGTAATCGATC+CGG | 0.549158 | 6.3:+38312048 | MS.gene80458:intron |
| TCGTGATAAAGGAAATTGGA+TGG | 0.565929 | 6.3:+38312428 | MS.gene80458:CDS |
| CGATGTAATCGATCCGGTGT+TGG | 0.566499 | 6.3:+38312054 | MS.gene80458:CDS |
| GTTCTGCTACGGCTGTTGGA+AGG | 0.576179 | 6.3:+38312209 | MS.gene80458:CDS |
| AGGGCAAAAGAACTCAAAGC+TGG | 0.576793 | 6.3:+38312486 | MS.gene80458:CDS |
| GCATAGTATTAGTTCTGCTA+CGG | 0.580120 | 6.3:+38312198 | MS.gene80458:CDS |
| AATTGGATGGAGAAATACGT+TGG | 0.583531 | 6.3:+38312441 | MS.gene80458:CDS |
| TGAATCTTGGTATTGCTCGA+AGG | 0.587394 | 6.3:+38311667 | MS.gene80458:CDS |
| GGCATCAATTCGTCCAACAC+CGG | 0.589626 | 6.3:-38312067 | None:intergenic |
| ATGGAGAAATACGTTGGAAG+CGG | 0.596476 | 6.3:+38312447 | MS.gene80458:CDS |
| AGAAATACGTTGGAAGCGGA+TGG | 0.602100 | 6.3:+38312451 | MS.gene80458:CDS |
| ACTGATGCAAATAATTTGTG+TGG | 0.631126 | 6.3:-38312539 | None:intergenic |
| AAATTGGTGTTACCTGCTAG+AGG | 0.647410 | 6.3:-38311730 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAACAATTTTTTTTTTAC+AGG | - | chr6.3:38311825-38311844 | None:intergenic | 10.0% |
| !!! | AAAACAATTTTTTTTTTACA+GGG | - | chr6.3:38311824-38311843 | None:intergenic | 10.0% |
| !!! | TGTAAAAAAAAAATTGTTTT+TGG | + | chr6.3:38311824-38311843 | MS.gene80458:intron | 10.0% |
| !! | TGAATAAATAACACAAAAAT+TGG | - | chr6.3:38311749-38311768 | None:intergenic | 15.0% |
| !!! | AAAAAACAAAAAATTTTGCA+GGG | - | chr6.3:38311851-38311870 | None:intergenic | 15.0% |
| !!! | ATTTTTGTGTTATTTATTCA+AGG | + | chr6.3:38311749-38311768 | MS.gene80458:intron | 15.0% |
| !!! | TAAAAAACAAAAAATTTTGC+AGG | - | chr6.3:38311852-38311871 | None:intergenic | 15.0% |
| !! | ATAACCAAATGATTGAATAA+AGG | - | chr6.3:38312009-38312028 | None:intergenic | 20.0% |
| !! | ATTGAATAAAGGCTAAAATA+TGG | - | chr6.3:38311998-38312017 | None:intergenic | 20.0% |
| !! | ATTTATTCAAGGCTAAAATA+TGG | + | chr6.3:38311760-38311779 | MS.gene80458:intron | 20.0% |
| !!! | CAAAAACAAAATATTTTGCA+GGG | + | chr6.3:38311897-38311916 | MS.gene80458:intron | 20.0% |
| !!! | CAGTTTCAAAATTTTCAAAA+TGG | + | chr6.3:38312157-38312176 | MS.gene80458:CDS | 20.0% |
| !!! | GCAATTTTATTTGTAATTTG+TGG | + | chr6.3:38312267-38312286 | MS.gene80458:intron | 20.0% |
| !!! | TCAAAAACAAAATATTTTGC+AGG | + | chr6.3:38311896-38311915 | MS.gene80458:intron | 20.0% |
| ! | AAATGCTTGGGTTATTATAA+GGG | + | chr6.3:38311638-38311657 | MS.gene80458:CDS | 25.0% |
| !! | AAAATGGTGATCATACTTTT+TGG | + | chr6.3:38312173-38312192 | MS.gene80458:CDS | 25.0% |
| !! | ATTGATTCACATACATACTT+TGG | - | chr6.3:38312356-38312375 | None:intergenic | 25.0% |
| !! | TGAACCCATTTTGTAATTTT+TGG | - | chr6.3:38311924-38311943 | None:intergenic | 25.0% |
| !! | TTAGCCTTTATTCAATCATT+TGG | + | chr6.3:38312002-38312021 | MS.gene80458:intron | 25.0% |
| !! | TTTGAAACTGTGCAAATATT+GGG | - | chr6.3:38312147-38312166 | None:intergenic | 25.0% |
| !!! | TTGTTTTTTATAATAGTCCC+TGG | + | chr6.3:38311862-38311881 | MS.gene80458:intron | 25.0% |
| !!! | TTTTGAAACTGTGCAAATAT+TGG | - | chr6.3:38312148-38312167 | None:intergenic | 25.0% |
| AAGTTCGTGATAAAGGAAAT+TGG | + | chr6.3:38312424-38312443 | MS.gene80458:CDS | 30.0% | |
| AGAGGATAGGTTAAAAAGAT+CGG | + | chr6.3:38311518-38311537 | MS.gene80458:CDS | 30.0% | |
| AGGGACCAAAAATTACAAAA+TGG | + | chr6.3:38311916-38311935 | MS.gene80458:intron | 30.0% | |
| ATAACCCAAGCATTTCTAAA+TGG | - | chr6.3:38311633-38311652 | None:intergenic | 30.0% | |
| ATAAGGGTTGTATTGAATCT+TGG | + | chr6.3:38311654-38311673 | MS.gene80458:CDS | 30.0% | |
| CGAATCGTTTATGATGAAAA+TGG | + | chr6.3:38312384-38312403 | MS.gene80458:CDS | 30.0% | |
| GAAATGCTTGGGTTATTATA+AGG | + | chr6.3:38311637-38311656 | MS.gene80458:CDS | 30.0% | |
| GGGACCAAAAATTACAAAAT+GGG | + | chr6.3:38311917-38311936 | MS.gene80458:intron | 30.0% | |
| TAACCCAAGCATTTCTAAAT+GGG | - | chr6.3:38311632-38311651 | None:intergenic | 30.0% | |
| ! | AAAAGATCGGGTCATTATTT+TGG | + | chr6.3:38311531-38311550 | MS.gene80458:CDS | 30.0% |
| ! | AAAGATCGGGTCATTATTTT+GGG | + | chr6.3:38311532-38311551 | MS.gene80458:CDS | 30.0% |
| ! | ACTGATGCAAATAATTTGTG+TGG | - | chr6.3:38312542-38312561 | None:intergenic | 30.0% |
| ! | TTTTTATAATAGTCCCTGGA+GGG | + | chr6.3:38311866-38311885 | MS.gene80458:intron | 30.0% |
| !!! | AAATGGGTTCAGTTTTTTAC+AGG | + | chr6.3:38311933-38311952 | MS.gene80458:intron | 30.0% |
| !!! | AATGGGTTCAGTTTTTTACA+GGG | + | chr6.3:38311934-38311953 | MS.gene80458:intron | 30.0% |
| !!! | TTTTTTATAATAGTCCCTGG+AGG | + | chr6.3:38311865-38311884 | MS.gene80458:intron | 30.0% |
| AATTGGATGGAGAAATACGT+TGG | + | chr6.3:38312441-38312460 | MS.gene80458:CDS | 35.0% | |
| AGGCTGAGAAGAAAAATCTT+AGG | + | chr6.3:38311687-38311706 | MS.gene80458:CDS | 35.0% | |
| CAAAACGAGGTATATTTGCA+GGG | + | chr6.3:38311965-38311984 | MS.gene80458:intron | 35.0% | |
| CATCCCATTTAGAAATGCTT+GGG | + | chr6.3:38311626-38311645 | MS.gene80458:CDS | 35.0% | |
| GAATGTGAAGTTCGTGATAA+AGG | + | chr6.3:38312417-38312436 | MS.gene80458:CDS | 35.0% | |
| GAGGATAGGTTAAAAAGATC+GGG | + | chr6.3:38311519-38311538 | MS.gene80458:CDS | 35.0% | |
| GCTTTCAAGGCAAAAAGATT+GGG | + | chr6.3:38311566-38311585 | MS.gene80458:CDS | 35.0% | |
| TCGTGATAAAGGAAATTGGA+TGG | + | chr6.3:38312428-38312447 | MS.gene80458:CDS | 35.0% | |
| TGAAGTTAGTGAAAGAGGAT+AGG | + | chr6.3:38311505-38311524 | MS.gene80458:CDS | 35.0% | |
| ! | AAGATTGGGCTTTTTACGTA+TGG | + | chr6.3:38311580-38311599 | MS.gene80458:CDS | 35.0% |
| ! | CAATCTTTTTGCCTTGAAAG+CGG | - | chr6.3:38311567-38311586 | None:intergenic | 35.0% |
| ! | GATTTTCTTCTACCGGAAAT+GGG | - | chr6.3:38312102-38312121 | None:intergenic | 35.0% |
| ! | TCGTTTGGATTTTCTTCTAC+CGG | - | chr6.3:38312109-38312128 | None:intergenic | 35.0% |
| !! | CGGATGGTTTGATTTTGTTA+GGG | + | chr6.3:38312467-38312486 | MS.gene80458:CDS | 35.0% |
| !! | GCATAGTATTAGTTCTGCTA+CGG | + | chr6.3:38312198-38312217 | MS.gene80458:CDS | 35.0% |
| !! | TTTTGAAAATAGTCCCTCCA+GGG | - | chr6.3:38311882-38311901 | None:intergenic | 35.0% |
| !! | TTTTTGAAAATAGTCCCTCC+AGG | - | chr6.3:38311883-38311902 | None:intergenic | 35.0% |
| ACCGGAAATGGGTGAAAATT+GGG | - | chr6.3:38312091-38312110 | None:intergenic | 40.0% | |
| ATGGAGAAATACGTTGGAAG+CGG | + | chr6.3:38312447-38312466 | MS.gene80458:CDS | 40.0% | |
| CAAAACGAGGCATATTTGCA+GGG | - | chr6.3:38311793-38311812 | None:intergenic | 40.0% | |
| CCAAAACGAGGTATATTTGC+AGG | + | chr6.3:38311964-38311983 | MS.gene80458:intron | 40.0% | |
| CCAAACGAAGAAGAAGTTGA+TGG | + | chr6.3:38312121-38312140 | MS.gene80458:CDS | 40.0% | |
| CCATCAACTTCTTCTTCGTT+TGG | - | chr6.3:38312124-38312143 | None:intergenic | 40.0% | |
| CGCTTTCAAGGCAAAAAGAT+TGG | + | chr6.3:38311565-38311584 | MS.gene80458:CDS | 40.0% | |
| CTTTGGATTGCAATCTTCTC+AGG | - | chr6.3:38312339-38312358 | None:intergenic | 40.0% | |
| GCATCCCATTTAGAAATGCT+TGG | + | chr6.3:38311625-38311644 | MS.gene80458:CDS | 40.0% | |
| TACCGGAAATGGGTGAAAAT+TGG | - | chr6.3:38312092-38312111 | None:intergenic | 40.0% | |
| TGAAAATGTCTTCCTCTAGC+AGG | + | chr6.3:38311718-38311737 | MS.gene80458:CDS | 40.0% | |
| TGAATCTTGGTATTGCTCGA+AGG | + | chr6.3:38311667-38311686 | MS.gene80458:CDS | 40.0% | |
| ! | CCTGCAAATATACCTCGTTT+TGG | - | chr6.3:38311967-38311986 | None:intergenic | 40.0% |
| ! | GGATTTTCTTCTACCGGAAA+TGG | - | chr6.3:38312103-38312122 | None:intergenic | 40.0% |
| ! | TTTTTGCCTTGAAAGCGGAT+CGG | - | chr6.3:38311562-38311581 | None:intergenic | 40.0% |
| !! | AAATTGGTGTTACCTGCTAG+AGG | - | chr6.3:38311733-38311752 | None:intergenic | 40.0% |
| !! | GCGGATGGTTTGATTTTGTT+AGG | + | chr6.3:38312466-38312485 | MS.gene80458:CDS | 40.0% |
| AGAAATACGTTGGAAGCGGA+TGG | + | chr6.3:38312451-38312470 | MS.gene80458:CDS | 45.0% | |
| AGGGCAAAAGAACTCAAAGC+TGG | + | chr6.3:38312486-38312505 | MS.gene80458:CDS | 45.0% | |
| ATGGACCGGGCAAACAATTT+CGG | + | chr6.3:38311599-38311618 | MS.gene80458:CDS | 45.0% | |
| CAGGGACTAAAACCAAAACG+AGG | + | chr6.3:38311952-38311971 | MS.gene80458:intron | 45.0% | |
| CAGGGACTAAAACCAAAACG+AGG | - | chr6.3:38311971-38311952 | None:intergenic | 45.0% | |
| CATCGCTGCAGTTACAACAA+CGG | - | chr6.3:38312039-38312058 | None:intergenic | 45.0% | |
| CCAAAACGAGGCATATTTGC+AGG | - | chr6.3:38311794-38311813 | None:intergenic | 45.0% | |
| GTTGGAAGGAATGTTCTGGT+TGG | + | chr6.3:38312223-38312242 | MS.gene80458:intron | 45.0% | |
| ! | CCTGCAAATATGCCTCGTTT+TGG | + | chr6.3:38311791-38311810 | MS.gene80458:intron | 45.0% |
| ! | GCCCAATTTTCACCCATTTC+CGG | + | chr6.3:38312087-38312106 | MS.gene80458:CDS | 45.0% |
| ! | TGGGCTTTTTACGTATGGAC+CGG | + | chr6.3:38311585-38311604 | MS.gene80458:CDS | 45.0% |
| !! | ATTAGTTCTGCTACGGCTGT+TGG | + | chr6.3:38312205-38312224 | MS.gene80458:CDS | 45.0% |
| CGATGTAATCGATCCGGTGT+TGG | + | chr6.3:38312054-38312073 | MS.gene80458:CDS | 50.0% | |
| CTGCAGCGATGTAATCGATC+CGG | + | chr6.3:38312048-38312067 | MS.gene80458:intron | 50.0% | |
| GGCATCAATTCGTCCAACAC+CGG | - | chr6.3:38312070-38312089 | None:intergenic | 50.0% | |
| GGCTGTTGGAAGGAATGTTC+TGG | + | chr6.3:38312219-38312238 | MS.gene80458:CDS | 50.0% | |
| TGGACCGGGCAAACAATTTC+GGG | + | chr6.3:38311600-38311619 | MS.gene80458:CDS | 50.0% | |
| ! | GGGCTTTTTACGTATGGACC+GGG | + | chr6.3:38311586-38311605 | MS.gene80458:CDS | 50.0% |
| ! | GGTTTTCCGATCCGCTTTCA+AGG | + | chr6.3:38311553-38311572 | MS.gene80458:CDS | 50.0% |
| GCGACCCGAAATTGTTTGCC+CGG | - | chr6.3:38311607-38311626 | None:intergenic | 55.0% | |
| GTTCTGCTACGGCTGTTGGA+AGG | + | chr6.3:38312209-38312228 | MS.gene80458:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 38311504 | 38312563 | 38311504 | ID=MS.gene80458 |
| chr6.3 | mRNA | 38311504 | 38312563 | 38311504 | ID=MS.gene80458.t1;Parent=MS.gene80458 |
| chr6.3 | exon | 38311504 | 38311739 | 38311504 | ID=MS.gene80458.t1.exon1;Parent=MS.gene80458.t1 |
| chr6.3 | CDS | 38311504 | 38311739 | 38311504 | ID=cds.MS.gene80458.t1;Parent=MS.gene80458.t1 |
| chr6.3 | exon | 38312054 | 38312240 | 38312054 | ID=MS.gene80458.t1.exon2;Parent=MS.gene80458.t1 |
| chr6.3 | CDS | 38312054 | 38312240 | 38312054 | ID=cds.MS.gene80458.t1;Parent=MS.gene80458.t1 |
| chr6.3 | exon | 38312330 | 38312563 | 38312330 | ID=MS.gene80458.t1.exon3;Parent=MS.gene80458.t1 |
| chr6.3 | CDS | 38312330 | 38312563 | 38312330 | ID=cds.MS.gene80458.t1;Parent=MS.gene80458.t1 |
| Gene Sequence |
| Protein sequence |
