AlfalfaGEDB | MS.gene80495

M. sativa cultivar XinJiangDaYe / MS.gene80495

KEGG: -
GO: -
PFAM: PF03634.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80495.t1	XP_027366958.1	73.3	105	28	0	1	105	78	182	5.80E-35	156.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80495.t1	O64647	65.3	101	34	1	6	105	71	171	2.0e-28	126.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80495.t1	A0A151U6N1	72.4	105	29	0	1	105	1	105	7.1e-35	156.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene80495.t1	TF	TCP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80495.t1	MTR_1g101810	72.381	105	29	0	1	105	55	159	4.40e-50	161
MS.gene80495.t1	MTR_1g101810	72.381	105	29	0	1	105	85	189	1.04e-49	161
MS.gene80495.t1	MTR_8g033070	67.391	92	29	1	10	101	80	170	5.23e-38	130
MS.gene80495.t1	MTR_7g028160	64.557	79	28	0	10	88	50	128	4.09e-31	111
MS.gene80495.t1	MTR_2g006150	58.242	91	38	0	7	97	127	217	1.27e-28	107
MS.gene80495.t1	MTR_2g006150	58.242	91	38	0	7	97	127	217	2.58e-28	106
MS.gene80495.t1	MTR_1g038650	64.286	70	25	0	3	72	25	94	4.40e-28	102
MS.gene80495.t1	MTR_1g114380	62.857	70	26	0	3	72	26	95	3.14e-27	100
MS.gene80495.t1	MTR_1g063870	69.841	63	19	0	10	72	49	111	7.96e-27	98.2
MS.gene80495.t1	MTR_5g039600	56.977	86	37	0	6	91	63	148	1.40e-26	101
MS.gene80495.t1	MTR_4g108370	68.919	74	17	1	5	72	86	159	2.89e-26	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80495.t1	AT2G45680	68.041	97	30	1	10	105	75	171	2.80e-39	134
MS.gene80495.t1	AT5G51910	71.429	84	23	1	5	87	51	134	2.28e-36	125
MS.gene80495.t1	AT5G51910	71.429	84	23	1	5	87	51	134	2.28e-36	125
MS.gene80495.t1	AT1G35560	72.727	66	18	0	7	72	52	117	9.10e-29	106
MS.gene80495.t1	AT1G69690	68.000	75	18	1	4	72	40	114	3.30e-28	104
MS.gene80495.t1	AT1G72010	73.913	69	18	0	4	72	57	125	5.44e-28	104
MS.gene80495.t1	AT3G27010	74.194	62	16	0	10	71	78	139	9.39e-28	103
MS.gene80495.t1	AT3G27010	74.194	62	16	0	10	71	78	139	9.39e-28	103
MS.gene80495.t1	AT5G08330	65.217	69	24	0	4	72	25	93	1.56e-27	100
MS.gene80495.t1	AT5G23280	63.380	71	26	0	2	72	31	101	1.97e-27	100
MS.gene80495.t1	AT1G58100	54.945	91	41	0	7	97	57	147	7.05e-26	99.4
MS.gene80495.t1	AT5G41030	62.319	69	26	0	7	75	65	133	1.03e-25	96.3
MS.gene80495.t1	AT3G47620	65.789	76	20	1	3	72	104	179	1.67e-25	99.4
MS.gene80495.t1	AT2G37000	71.186	59	17	0	10	68	43	101	1.15e-24	92.4
MS.gene80495.t1	AT3G45150	52.542	59	28	0	11	69	18	76	3.40e-18	75.1

Find 36 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGGTGGCTTTGATGATTGC+AGG	0.311422	6.3:+37185014	None:intergenic
GGCTATGGCCGGAATTATTC+CGG	0.369975	6.3:+37184989	None:intergenic
AGAAACAATAGGGTGGTTAC+TGG	0.376057	6.3:-37185048	MS.gene80495:CDS
GGTCTAGCTGCTGATGTTGT+TGG	0.394840	6.3:+37184939	None:intergenic
CAACTTTCCGCCCTCTGAAA+TGG	0.397867	6.3:+37184968	None:intergenic
CACCGGCACCGGAATAATTC+CGG	0.398178	6.3:-37184997	MS.gene80495:CDS
GACCAAGATCTCTCGTCAAC+TGG	0.398427	6.3:+37185084	None:intergenic
AAGTCTGATGGAGAAACAAT+AGG	0.400346	6.3:-37185059	MS.gene80495:CDS
TTCCAGTTGACGAGAGATCT+TGG	0.406107	6.3:-37185086	MS.gene80495:CDS
TTCAGAGGGCGGAAAGTTGA+AGG	0.466569	6.3:-37184964	MS.gene80495:CDS
ATCCACAAATTAACCAATTG+AGG	0.488844	6.3:+37184878	None:intergenic
TTCGTGTGACGGTCTTCTGA+TGG	0.489473	6.3:+37185164	None:intergenic
AGCTAGACCAGACGGCCAAA+AGG	0.496901	6.3:-37184925	MS.gene80495:CDS
ATCATCAAAGCCACCGGCAC+CGG	0.497106	6.3:-37185008	MS.gene80495:CDS
CCCTCTGAAATGGCTATGGC+CGG	0.502325	6.3:+37184978	None:intergenic
TGTCAGCAACATGTGCATCT+AGG	0.504146	6.3:-37185112	MS.gene80495:CDS
AAAAGCAAGAGAGCCTCAAT+TGG	0.505951	6.3:-37184891	MS.gene80495:CDS
GGCCGGAATTATTCCGGTGC+CGG	0.511782	6.3:+37184995	None:intergenic
AGTCTGATGGAGAAACAATA+GGG	0.517180	6.3:-37185058	MS.gene80495:CDS
TCCGCCCTCTGAAATGGCTA+TGG	0.528278	6.3:+37184974	None:intergenic
TTGCTGACAGTGTAACTCTT+CGG	0.528603	6.3:+37185126	None:intergenic
CGGAATTATTCCGGTGCCGG+TGG	0.529649	6.3:+37184998	None:intergenic
GTCCCTCCACCTTCGTGTGA+CGG	0.543321	6.3:+37185153	None:intergenic
GATCTTGGTCACAAGTCTGA+TGG	0.556205	6.3:-37185071	MS.gene80495:CDS
AGACCGTCACACGAAGGTGG+AGG	0.561466	6.3:-37185156	MS.gene80495:CDS
CAGACGGCCAAAAGGCCCCA+AGG	0.564342	6.3:-37184917	MS.gene80495:CDS
GACCGTCACACGAAGGTGGA+GGG	0.603506	6.3:-37185155	MS.gene80495:CDS
TCCGGCCATAGCCATTTCAG+AGG	0.619818	6.3:-37184979	MS.gene80495:CDS
GCCATAGCCATTTCAGAGGG+CGG	0.621097	6.3:-37184975	MS.gene80495:CDS
AGAAGACCGTCACACGAAGG+TGG	0.622175	6.3:-37185159	MS.gene80495:CDS
CCTGCAATCATCAAAGCCAC+CGG	0.628378	6.3:-37185014	MS.gene80495:CDS
CCGGCCATAGCCATTTCAGA+GGG	0.636688	6.3:-37184978	MS.gene80495:CDS
ACATCAGCAGCTAGACCAGA+CGG	0.655173	6.3:-37184933	MS.gene80495:CDS
GGAACCACAAATGACAGTGT+CGG	0.659107	6.3:-37185198	None:intergenic
ATCAGAAGACCGTCACACGA+AGG	0.685962	6.3:-37185162	MS.gene80495:CDS
CTGATGGAGAAACAATAGGG+TGG	0.705438	6.3:-37185055	MS.gene80495:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTCTGATGGAGAAACAAT+AGG	-	chr6.3:37185016-37185035	MS.gene80495:CDS	35.0%
	AGTCTGATGGAGAAACAATA+GGG	-	chr6.3:37185017-37185036	MS.gene80495:CDS	35.0%
	AAAAGCAAGAGAGCCTCAAT+TGG	-	chr6.3:37185184-37185203	MS.gene80495:CDS	40.0%
	AGAAACAATAGGGTGGTTAC+TGG	-	chr6.3:37185027-37185046	MS.gene80495:CDS	40.0%
	TTGCTGACAGTGTAACTCTT+CGG	+	chr6.3:37184952-37184971	None:intergenic	40.0%
!!	CTCTCTTGCTTTTCTTCCTT+GGG	+	chr6.3:37185177-37185196	None:intergenic	40.0%
!!	TCTCTTGCTTTTCTTCCTTG+GGG	+	chr6.3:37185176-37185195	None:intergenic	40.0%
	CTGATGGAGAAACAATAGGG+TGG	-	chr6.3:37185020-37185039	MS.gene80495:CDS	45.0%
	GATCTTGGTCACAAGTCTGA+TGG	-	chr6.3:37185004-37185023	MS.gene80495:CDS	45.0%
	TGTCAGCAACATGTGCATCT+AGG	-	chr6.3:37184963-37184982	MS.gene80495:CDS	45.0%
	TTCCAGTTGACGAGAGATCT+TGG	-	chr6.3:37184989-37185008	MS.gene80495:CDS	45.0%
!	TTTTCTTCCTTGGGGCCTTT+TGG	+	chr6.3:37185168-37185187	None:intergenic	45.0%
!!	GCTCTCTTGCTTTTCTTCCT+TGG	+	chr6.3:37185178-37185197	None:intergenic	45.0%
	ACATCAGCAGCTAGACCAGA+CGG	-	chr6.3:37185142-37185161	MS.gene80495:CDS	50.0%
	ATCAGAAGACCGTCACACGA+AGG	-	chr6.3:37184913-37184932	MS.gene80495:CDS	50.0%
	CCTGCAATCATCAAAGCCAC+CGG	-	chr6.3:37185061-37185080	MS.gene80495:CDS	50.0%
	GACCAAGATCTCTCGTCAAC+TGG	+	chr6.3:37184994-37185013	None:intergenic	50.0%
	GGCTATGGCCGGAATTATTC+CGG	+	chr6.3:37185089-37185108	None:intergenic	50.0%
	GGTCTAGCTGCTGATGTTGT+TGG	+	chr6.3:37185139-37185158	None:intergenic	50.0%
	TTCAGAGGGCGGAAAGTTGA+AGG	-	chr6.3:37185111-37185130	MS.gene80495:CDS	50.0%
	TTCGTGTGACGGTCTTCTGA+TGG	+	chr6.3:37184914-37184933	None:intergenic	50.0%
!	CAACTTTCCGCCCTCTGAAA+TGG	+	chr6.3:37185110-37185129	None:intergenic	50.0%
	AGAAGACCGTCACACGAAGG+TGG	-	chr6.3:37184916-37184935	MS.gene80495:CDS	55.0%
	AGCTAGACCAGACGGCCAAA+AGG	-	chr6.3:37185150-37185169	MS.gene80495:CDS	55.0%
	ATCATCAAAGCCACCGGCAC+CGG	-	chr6.3:37185067-37185086	MS.gene80495:CDS	55.0%
	CCGGCCATAGCCATTTCAGA+GGG	-	chr6.3:37185097-37185116	MS.gene80495:CDS	55.0%
	GCCATAGCCATTTCAGAGGG+CGG	-	chr6.3:37185100-37185119	MS.gene80495:CDS	55.0%
	TCCGGCCATAGCCATTTCAG+AGG	-	chr6.3:37185096-37185115	MS.gene80495:CDS	55.0%
!	CACCGGCACCGGAATAATTC+CGG	-	chr6.3:37185078-37185097	MS.gene80495:CDS	55.0%
!!	CCCTCTGAAATGGCTATGGC+CGG	+	chr6.3:37185100-37185119	None:intergenic	55.0%
!!	CCGGTGGCTTTGATGATTGC+AGG	+	chr6.3:37185064-37185083	None:intergenic	55.0%
!!	TCCGCCCTCTGAAATGGCTA+TGG	+	chr6.3:37185104-37185123	None:intergenic	55.0%
	AGACCGTCACACGAAGGTGG+AGG	-	chr6.3:37184919-37184938	MS.gene80495:CDS	60.0%
	GACCGTCACACGAAGGTGGA+GGG	-	chr6.3:37184920-37184939	MS.gene80495:CDS	60.0%
	GGCCGGAATTATTCCGGTGC+CGG	+	chr6.3:37185083-37185102	None:intergenic	60.0%
	GTCCCTCCACCTTCGTGTGA+CGG	+	chr6.3:37184925-37184944	None:intergenic	60.0%
!	CGGAATTATTCCGGTGCCGG+TGG	+	chr6.3:37185080-37185099	None:intergenic	60.0%
	CAGACGGCCAAAAGGCCCCA+AGG	-	chr6.3:37185158-37185177	MS.gene80495:CDS	65.0%
!	CTTGGGGCCTTTTGGCCGTC+TGG	+	chr6.3:37185160-37185179	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	37184887	37185210	37184887	ID=MS.gene80495
chr6.3	mRNA	37184887	37185210	37184887	ID=MS.gene80495.t1;Parent=MS.gene80495
chr6.3	exon	37184887	37185210	37184887	ID=MS.gene80495.t1.exon1;Parent=MS.gene80495.t1
chr6.3	CDS	37184887	37185210	37184887	ID=cds.MS.gene80495.t1;Parent=MS.gene80495.t1

Gene Sequence	>MS.gene80495 ATGACAGTGTCGGCTGAAAAGAAACCATCAGAAGACCGTCACACGAAGGTGGAGGGACGTGTCCGAAGAGTTACACTGTCAGCAACATGTGCATCTAGGATCTTCCAGTTGACGAGAGATCTTGGTCACAAGTCTGATGGAGAAACAATAGGGTGGTTACTGGAACAAGCTGAACCTGCAATCATCAAAGCCACCGGCACCGGAATAATTCCGGCCATAGCCATTTCAGAGGGCGGAAAGTTGAAGGTTCCAACAACATCAGCAGCTAGACCAGACGGCCAAAAGGCCCCAAGGAAGAAAAGCAAGAGAGCCTCAATTGGTTAA
Protein sequence	>MS.gene80495.t1 MTVSAEKKPSEDRHTKVEGRVRRVTLSATCASRIFQLTRDLGHKSDGETIGWLLEQAEPAIIKATGTGIIPAIAISEGGKLKVPTTSAARPDGQKAPRKKSKRASIG