AlfalfaGEDB | MS.gene80497

M. sativa cultivar XinJiangDaYe / MS.gene80497

KEGG: -
GO: -
PFAM: PF03634.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80497.t1	XP_027366958.1	74.3	101	26	0	17	117	82	182	1.60E-33	152.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80497.t1	Q9LT89	64.4	104	36	1	3	105	37	140	5.9e-29	128.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80497.t1	A0A1S2XYS3	73.7	99	26	0	19	117	71	169	1.1e-33	152.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene80497.t1	TF	TCP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80497.t1	MTR_1g101810	66.667	117	38	1	2	117	43	159	1.22e-48	158
MS.gene80497.t1	MTR_1g101810	66.667	117	38	1	2	117	73	189	3.23e-48	158
MS.gene80497.t1	MTR_8g033070	66.304	92	30	1	22	113	80	170	1.20e-36	127
MS.gene80497.t1	MTR_7g028160	58.242	91	38	0	10	100	38	128	9.82e-33	115
MS.gene80497.t1	MTR_2g006150	74.242	66	17	0	19	84	127	192	7.62e-30	111
MS.gene80497.t1	MTR_1g038650	55.682	88	37	1	12	97	22	109	5.36e-29	105
MS.gene80497.t1	MTR_1g114380	60.274	73	29	0	12	84	23	95	3.61e-27	100
MS.gene80497.t1	MTR_2g006150	58.242	91	38	0	19	109	127	217	4.89e-27	103
MS.gene80497.t1	MTR_1g063870	69.841	63	19	0	22	84	49	111	1.13e-26	98.6
MS.gene80497.t1	MTR_5g039600	64.000	75	27	0	10	84	55	129	1.35e-26	102
MS.gene80497.t1	MTR_4g108370	65.000	80	22	1	11	84	80	159	8.64e-26	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80497.t1	AT2G45680	67.010	97	31	1	22	117	75	171	5.27e-38	131
MS.gene80497.t1	AT5G51910	65.000	100	34	1	1	99	35	134	1.04e-37	129
MS.gene80497.t1	AT5G51910	65.000	100	34	1	1	99	35	134	1.04e-37	129
MS.gene80497.t1	AT1G72010	73.913	69	18	0	16	84	57	125	3.86e-31	113
MS.gene80497.t1	AT1G69690	74.603	63	16	0	22	84	52	114	4.37e-29	107
MS.gene80497.t1	AT5G23280	61.842	76	29	0	9	84	26	101	1.30e-28	104
MS.gene80497.t1	AT1G58100	71.212	66	19	0	19	84	57	122	1.38e-28	107
MS.gene80497.t1	AT1G35560	72.727	66	18	0	19	84	52	117	1.40e-28	106
MS.gene80497.t1	AT3G47620	59.091	88	31	1	2	84	92	179	1.62e-28	108
MS.gene80497.t1	AT3G27010	65.278	72	25	0	12	83	68	139	3.10e-28	105
MS.gene80497.t1	AT3G27010	65.278	72	25	0	12	83	68	139	3.10e-28	105
MS.gene80497.t1	AT5G08330	65.217	69	24	0	16	84	25	93	2.45e-27	100
MS.gene80497.t1	AT5G41030	62.319	69	26	0	19	87	65	133	2.01e-25	96.3
MS.gene80497.t1	AT2G37000	71.186	59	17	0	22	80	43	101	1.55e-24	92.4
MS.gene80497.t1	AT3G45150	52.542	59	28	0	23	81	18	76	6.04e-18	75.1

Find 38 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGGTGGCTTTGATGATTGC+AGG	0.311422	6.3:+37166442	None:intergenic
GGCTATGGCCGGAATTATTC+CGG	0.369975	6.3:+37166417	None:intergenic
AGAAACAATAGGGTGGTTAC+TGG	0.376057	6.3:-37166476	MS.gene80497:CDS
TTCAGAGGGCGGAAAGTTGA+TGG	0.396962	6.3:-37166392	MS.gene80497:CDS
CACCGGCACCGGAATAATTC+CGG	0.398178	6.3:-37166425	MS.gene80497:CDS
GACCAAGATCTCTCGTCAAC+TGG	0.398427	6.3:+37166512	None:intergenic
AAGTCTGATGGAGAAACAAT+AGG	0.400346	6.3:-37166487	MS.gene80497:CDS
TTCCAGTTGACGAGAGATCT+TGG	0.406107	6.3:-37166514	MS.gene80497:CDS
CAACTTTCCGCCCTCTGAAA+TGG	0.406495	6.3:+37166396	None:intergenic
GGTCTAGCTGCCGATGTTGT+TGG	0.412258	6.3:+37166367	None:intergenic
TTGCTGACAGTGTGACTCTT+CGG	0.478708	6.3:+37166554	None:intergenic
TTCGTGTGACGGTCTTCTGA+TGG	0.489473	6.3:+37166592	None:intergenic
ATCATCAAAGCCACCGGCAC+CGG	0.497106	6.3:-37166436	MS.gene80497:CDS
AGCTAGACCAGACGGCCAAA+AGG	0.501805	6.3:-37166353	MS.gene80497:CDS
CCCTCTGAAATGGCTATGGC+CGG	0.502325	6.3:+37166406	None:intergenic
TGTCAGCAACATGTGCATCT+AGG	0.504146	6.3:-37166540	MS.gene80497:CDS
AAAAGCAAGAGAGCCTCAAT+TGG	0.505951	6.3:-37166319	MS.gene80497:CDS
GGCCGGAATTATTCCGGTGC+CGG	0.511782	6.3:+37166423	None:intergenic
AGTCTGATGGAGAAACAATA+GGG	0.517180	6.3:-37166486	MS.gene80497:CDS
TCCGCCCTCTGAAATGGCTA+TGG	0.528278	6.3:+37166402	None:intergenic
CGGAATTATTCCGGTGCCGG+TGG	0.529649	6.3:+37166426	None:intergenic
AAAACAGACGTTGAACCCAC+TGG	0.536961	6.3:-37166647	MS.gene80497:CDS
GTCCCTCCACCTTCGTGTGA+CGG	0.543321	6.3:+37166581	None:intergenic
GTCCACAAATTAACCAATTG+AGG	0.547161	6.3:+37166306	None:intergenic
GATCTTGGTCACAAGTCTGA+TGG	0.556205	6.3:-37166499	MS.gene80497:CDS
AGACCGTCACACGAAGGTGG+AGG	0.561466	6.3:-37166584	MS.gene80497:CDS
CAGACGGCCAAAAGGCCCCA+AGG	0.564342	6.3:-37166345	MS.gene80497:CDS
GACCGTCACACGAAGGTGGA+GGG	0.603506	6.3:-37166583	MS.gene80497:CDS
TCCGGCCATAGCCATTTCAG+AGG	0.619818	6.3:-37166407	MS.gene80497:CDS
GCCATAGCCATTTCAGAGGG+CGG	0.621097	6.3:-37166403	MS.gene80497:CDS
AGAAGACCGTCACACGAAGG+TGG	0.622175	6.3:-37166587	MS.gene80497:CDS
CCTGCAATCATCAAAGCCAC+CGG	0.628378	6.3:-37166442	MS.gene80497:CDS
CCGGCCATAGCCATTTCAGA+GGG	0.636688	6.3:-37166406	MS.gene80497:CDS
ACATCGGCAGCTAGACCAGA+CGG	0.655173	6.3:-37166361	MS.gene80497:CDS
GTTGATGGTTCCAACAACAT+CGG	0.672071	6.3:-37166377	MS.gene80497:CDS
GGAACCACAAAAGACAGTGT+CGG	0.676346	6.3:-37166626	MS.gene80497:CDS
ATCAGAAGACCGTCACACGA+AGG	0.685962	6.3:-37166590	MS.gene80497:CDS
CTGATGGAGAAACAATAGGG+TGG	0.705438	6.3:-37166483	MS.gene80497:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTCTGATGGAGAAACAAT+AGG	-	chr6.3:37166480-37166499	MS.gene80497:CDS	35.0%
	AGTCTGATGGAGAAACAATA+GGG	-	chr6.3:37166481-37166500	MS.gene80497:CDS	35.0%
!	GTCTTCTGATGGTTTCTTTT+CGG	+	chr6.3:37166367-37166386	None:intergenic	35.0%
	AAAAGCAAGAGAGCCTCAAT+TGG	-	chr6.3:37166648-37166667	MS.gene80497:CDS	40.0%
	AGAAACAATAGGGTGGTTAC+TGG	-	chr6.3:37166491-37166510	MS.gene80497:CDS	40.0%
!!	CTCTCTTGCTTTTCTTCCTT+GGG	+	chr6.3:37166641-37166660	None:intergenic	40.0%
!!	GTTGATGGTTCCAACAACAT+CGG	-	chr6.3:37166590-37166609	MS.gene80497:CDS	40.0%
!!	TCTCTTGCTTTTCTTCCTTG+GGG	+	chr6.3:37166640-37166659	None:intergenic	40.0%
	AAAACAGACGTTGAACCCAC+TGG	-	chr6.3:37166320-37166339	MS.gene80497:CDS	45.0%
	CTGATGGAGAAACAATAGGG+TGG	-	chr6.3:37166484-37166503	MS.gene80497:CDS	45.0%
	GATCTTGGTCACAAGTCTGA+TGG	-	chr6.3:37166468-37166487	MS.gene80497:CDS	45.0%
	GGAACCACAAAAGACAGTGT+CGG	-	chr6.3:37166341-37166360	MS.gene80497:CDS	45.0%
	TGTCAGCAACATGTGCATCT+AGG	-	chr6.3:37166427-37166446	MS.gene80497:CDS	45.0%
	TTCCAGTTGACGAGAGATCT+TGG	-	chr6.3:37166453-37166472	MS.gene80497:CDS	45.0%
	TTGCTGACAGTGTGACTCTT+CGG	+	chr6.3:37166416-37166435	None:intergenic	45.0%
!	ACTGTCTTTTGTGGTTCCAG+TGG	+	chr6.3:37166339-37166358	None:intergenic	45.0%
!	CTGTCTTTTGTGGTTCCAGT+GGG	+	chr6.3:37166338-37166357	None:intergenic	45.0%
!	TTTTCTTCCTTGGGGCCTTT+TGG	+	chr6.3:37166632-37166651	None:intergenic	45.0%
!!	GCTCTCTTGCTTTTCTTCCT+TGG	+	chr6.3:37166642-37166661	None:intergenic	45.0%
	ATCAGAAGACCGTCACACGA+AGG	-	chr6.3:37166377-37166396	MS.gene80497:CDS	50.0%
	CCTGCAATCATCAAAGCCAC+CGG	-	chr6.3:37166525-37166544	MS.gene80497:CDS	50.0%
	GACCAAGATCTCTCGTCAAC+TGG	+	chr6.3:37166458-37166477	None:intergenic	50.0%
	GGCTATGGCCGGAATTATTC+CGG	+	chr6.3:37166553-37166572	None:intergenic	50.0%
	TTCAGAGGGCGGAAAGTTGA+TGG	-	chr6.3:37166575-37166594	MS.gene80497:CDS	50.0%
	TTCGTGTGACGGTCTTCTGA+TGG	+	chr6.3:37166378-37166397	None:intergenic	50.0%
!	CAACTTTCCGCCCTCTGAAA+TGG	+	chr6.3:37166574-37166593	None:intergenic	50.0%
	ACATCGGCAGCTAGACCAGA+CGG	-	chr6.3:37166606-37166625	MS.gene80497:CDS	55.0%
	AGAAGACCGTCACACGAAGG+TGG	-	chr6.3:37166380-37166399	MS.gene80497:CDS	55.0%
	AGCTAGACCAGACGGCCAAA+AGG	-	chr6.3:37166614-37166633	MS.gene80497:CDS	55.0%
	ATCATCAAAGCCACCGGCAC+CGG	-	chr6.3:37166531-37166550	MS.gene80497:CDS	55.0%
	CCGGCCATAGCCATTTCAGA+GGG	-	chr6.3:37166561-37166580	MS.gene80497:CDS	55.0%
	GCCATAGCCATTTCAGAGGG+CGG	-	chr6.3:37166564-37166583	MS.gene80497:CDS	55.0%
	GGTCTAGCTGCCGATGTTGT+TGG	+	chr6.3:37166603-37166622	None:intergenic	55.0%
	TCCGGCCATAGCCATTTCAG+AGG	-	chr6.3:37166560-37166579	MS.gene80497:CDS	55.0%
!	CACCGGCACCGGAATAATTC+CGG	-	chr6.3:37166542-37166561	MS.gene80497:CDS	55.0%
!	TCGGCCGACACTGTCTTTTG+TGG	+	chr6.3:37166348-37166367	None:intergenic	55.0%
!!	CCCTCTGAAATGGCTATGGC+CGG	+	chr6.3:37166564-37166583	None:intergenic	55.0%
!!	CCGGTGGCTTTGATGATTGC+AGG	+	chr6.3:37166528-37166547	None:intergenic	55.0%
!!	TCCGCCCTCTGAAATGGCTA+TGG	+	chr6.3:37166568-37166587	None:intergenic	55.0%
	AGACCGTCACACGAAGGTGG+AGG	-	chr6.3:37166383-37166402	MS.gene80497:CDS	60.0%
	GACCGTCACACGAAGGTGGA+GGG	-	chr6.3:37166384-37166403	MS.gene80497:CDS	60.0%
	GGCCGGAATTATTCCGGTGC+CGG	+	chr6.3:37166547-37166566	None:intergenic	60.0%
	GTCCCTCCACCTTCGTGTGA+CGG	+	chr6.3:37166389-37166408	None:intergenic	60.0%
!	CGGAATTATTCCGGTGCCGG+TGG	+	chr6.3:37166544-37166563	None:intergenic	60.0%
	CAGACGGCCAAAAGGCCCCA+AGG	-	chr6.3:37166622-37166641	MS.gene80497:CDS	65.0%
!	CTTGGGGCCTTTTGGCCGTC+TGG	+	chr6.3:37166624-37166643	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	37166315	37166674	37166315	ID=MS.gene80497
chr6.3	mRNA	37166315	37166674	37166315	ID=MS.gene80497.t1;Parent=MS.gene80497
chr6.3	exon	37166315	37166674	37166315	ID=MS.gene80497.t1.exon1;Parent=MS.gene80497.t1
chr6.3	CDS	37166315	37166674	37166315	ID=cds.MS.gene80497.t1;Parent=MS.gene80497.t1

Gene Sequence	>MS.gene80497 ATGGAAAAACAGACGTTGAACCCACTGGAACCACAAAAGACAGTGTCGGCCGAAAAGAAACCATCAGAAGACCGTCACACGAAGGTGGAGGGACGTGTCCGAAGAGTCACACTGTCAGCAACATGTGCATCTAGGATCTTCCAGTTGACGAGAGATCTTGGTCACAAGTCTGATGGAGAAACAATAGGGTGGTTACTGGAACAAGCTGAACCTGCAATCATCAAAGCCACCGGCACCGGAATAATTCCGGCCATAGCCATTTCAGAGGGCGGAAAGTTGATGGTTCCAACAACATCGGCAGCTAGACCAGACGGCCAAAAGGCCCCAAGGAAGAAAAGCAAGAGAGCCTCAATTGGTTAA
Protein sequence	>MS.gene80497.t1 MEKQTLNPLEPQKTVSAEKKPSEDRHTKVEGRVRRVTLSATCASRIFQLTRDLGHKSDGETIGWLLEQAEPAIIKATGTGIIPAIAISEGGKLMVPTTSAARPDGQKAPRKKSKRASIG