AlfalfaGEDB | MS.gene80501

M. sativa cultivar XinJiangDaYe / MS.gene80501

KEGG: -
GO: -
PFAM: PF03634.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80501.t1	XP_027366958.1	75.2	101	25	0	17	117	82	182	1.20E-33	152.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80501.t1	Q9LT89	64.4	104	36	1	3	105	37	140	7.7e-29	127.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80501.t1	A0A151U6N1	72.1	104	29	0	14	117	2	105	1.1e-33	152.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene80501.t1	TF	TCP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80501.t1	MTR_1g101810	66.667	117	38	1	2	117	43	159	1.17e-48	158
MS.gene80501.t1	MTR_1g101810	66.667	117	38	1	2	117	73	189	2.96e-48	158
MS.gene80501.t1	MTR_8g033070	64.356	101	29	2	22	115	80	180	4.17e-38	131
MS.gene80501.t1	MTR_7g028160	58.242	91	38	0	10	100	38	128	1.02e-31	113
MS.gene80501.t1	MTR_2g006150	58.242	91	38	0	19	109	127	217	6.67e-30	111
MS.gene80501.t1	MTR_4g108370	65.000	80	22	1	11	84	80	159	8.42e-29	108
MS.gene80501.t1	MTR_1g038650	63.014	73	27	0	12	84	22	94	1.05e-28	104
MS.gene80501.t1	MTR_1g114380	60.274	73	29	0	12	84	23	95	4.32e-27	100
MS.gene80501.t1	MTR_2g006150	58.242	91	38	0	19	109	127	217	6.73e-27	103
MS.gene80501.t1	MTR_1g063870	69.841	63	19	0	22	84	49	111	1.52e-26	98.2
MS.gene80501.t1	MTR_5g039600	53.684	95	44	0	10	104	55	149	2.71e-26	101

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80501.t1	AT5G51910	66.000	100	33	1	1	99	35	134	1.51e-38	131
MS.gene80501.t1	AT5G51910	66.000	100	33	1	1	99	35	134	1.51e-38	131
MS.gene80501.t1	AT2G45680	67.010	97	31	1	22	117	75	171	4.89e-38	131
MS.gene80501.t1	AT1G72010	73.913	69	18	0	16	84	57	125	5.62e-31	113
MS.gene80501.t1	AT1G69690	74.603	63	16	0	22	84	52	114	7.32e-29	107
MS.gene80501.t1	AT1G35560	72.727	66	18	0	19	84	52	117	1.71e-28	106
MS.gene80501.t1	AT5G23280	61.842	76	29	0	9	84	26	101	1.75e-28	104
MS.gene80501.t1	AT1G58100	71.212	66	19	0	19	84	57	122	2.02e-28	107
MS.gene80501.t1	AT3G47620	59.091	88	31	1	2	84	92	179	2.45e-28	107
MS.gene80501.t1	AT3G27010	65.278	72	25	0	12	83	68	139	5.24e-28	104
MS.gene80501.t1	AT3G27010	65.278	72	25	0	12	83	68	139	5.24e-28	104
MS.gene80501.t1	AT5G08330	65.217	69	24	0	16	84	25	93	2.97e-27	100
MS.gene80501.t1	AT5G41030	62.319	69	26	0	19	87	65	133	1.93e-25	96.3
MS.gene80501.t1	AT2G37000	71.186	59	17	0	22	80	43	101	2.90e-24	92.0
MS.gene80501.t1	AT3G45150	52.542	59	28	0	23	81	18	76	1.27e-17	74.3

Find 38 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGGTGGCGTTGATGATTGC+AGG	0.349030	6.3:+37113684	None:intergenic
GGCTATGGCCGGAATTATTC+CGG	0.369975	6.3:+37113659	None:intergenic
AGAAACAATAGGGTGGTTAC+TGG	0.376057	6.3:-37113718	MS.gene80501:CDS
CACCGGCACCGGAATAATTC+CGG	0.395013	6.3:-37113667	MS.gene80501:CDS
CAACTTTCCGCCCTCTGAAA+TGG	0.397867	6.3:+37113638	None:intergenic
GACCAAGATCTCTCGTCAAC+TGG	0.398427	6.3:+37113754	None:intergenic
AAGTCTGATGGAGAAACAAT+AGG	0.400346	6.3:-37113729	MS.gene80501:CDS
TTCCAGTTGACGAGAGATCT+TGG	0.406107	6.3:-37113756	MS.gene80501:CDS
GGTCTAGCTGCCGATGTTGT+TGG	0.423161	6.3:+37113609	None:intergenic
TTCAGAGGGCGGAAAGTTGA+AGG	0.466569	6.3:-37113634	MS.gene80501:CDS
TTGCTGACAGTGTGACTCTT+CGG	0.478708	6.3:+37113796	None:intergenic
AGCTAGACCAAACGGCCAAA+AGG	0.481264	6.3:-37113595	MS.gene80501:CDS
ATCCACAAATTAACCAATTG+AGG	0.488844	6.3:+37113548	None:intergenic
TTCGTGTGACGGTCTTCTGA+TGG	0.489473	6.3:+37113834	None:intergenic
ATCATCAACGCCACCGGCAC+CGG	0.490098	6.3:-37113678	MS.gene80501:CDS
CCCTCTGAAATGGCTATGGC+CGG	0.502325	6.3:+37113648	None:intergenic
TGTCAGCAACATGTGCATCT+AGG	0.504146	6.3:-37113782	MS.gene80501:CDS
AAAAGCAAGAGAGCCTCAAT+TGG	0.505951	6.3:-37113561	MS.gene80501:CDS
GGCCGGAATTATTCCGGTGC+CGG	0.511782	6.3:+37113665	None:intergenic
AGTCTGATGGAGAAACAATA+GGG	0.517180	6.3:-37113728	MS.gene80501:CDS
TCCGCCCTCTGAAATGGCTA+TGG	0.528278	6.3:+37113644	None:intergenic
AAAACAGACGTTGAACCCGC+TGG	0.542316	6.3:-37113889	MS.gene80501:CDS
GTCCCTCCACCTTCGTGTGA+CGG	0.543321	6.3:+37113823	None:intergenic
CGGAATTATTCCGGTGCCGG+TGG	0.551061	6.3:+37113668	None:intergenic
GATCTTGGTCACAAGTCTGA+TGG	0.556205	6.3:-37113741	MS.gene80501:CDS
AGACCGTCACACGAAGGTGG+AGG	0.561466	6.3:-37113826	MS.gene80501:CDS
ACATCGGCAGCTAGACCAAA+CGG	0.561909	6.3:-37113603	MS.gene80501:CDS
CAAACGGCCAAAAGGCCCCA+AGG	0.576905	6.3:-37113587	MS.gene80501:CDS
CCTGCAATCATCAACGCCAC+CGG	0.585604	6.3:-37113684	MS.gene80501:CDS
GACCGTCACACGAAGGTGGA+GGG	0.603506	6.3:-37113825	MS.gene80501:CDS
TCCGGCCATAGCCATTTCAG+AGG	0.619818	6.3:-37113649	MS.gene80501:CDS
GCCATAGCCATTTCAGAGGG+CGG	0.621097	6.3:-37113645	MS.gene80501:CDS
AGAAGACCGTCACACGAAGG+TGG	0.622175	6.3:-37113829	MS.gene80501:CDS
CCGGCCATAGCCATTTCAGA+GGG	0.636688	6.3:-37113648	MS.gene80501:CDS
GTTGAAGGTTCCAACAACAT+CGG	0.665250	6.3:-37113619	MS.gene80501:CDS
ATCAGAAGACCGTCACACGA+AGG	0.685962	6.3:-37113832	MS.gene80501:CDS
GGAACCACAAAAGACAGTGT+CGG	0.689675	6.3:-37113868	MS.gene80501:CDS
CTGATGGAGAAACAATAGGG+TGG	0.705438	6.3:-37113725	MS.gene80501:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTCTGATGGAGAAACAAT+AGG	-	chr6.3:37113722-37113741	MS.gene80501:CDS	35.0%
	AGTCTGATGGAGAAACAATA+GGG	-	chr6.3:37113723-37113742	MS.gene80501:CDS	35.0%
!	GTCTTCTGATGGTTTCTTTT+CGG	+	chr6.3:37113609-37113628	None:intergenic	35.0%
	AAAAGCAAGAGAGCCTCAAT+TGG	-	chr6.3:37113890-37113909	MS.gene80501:CDS	40.0%
	AGAAACAATAGGGTGGTTAC+TGG	-	chr6.3:37113733-37113752	MS.gene80501:CDS	40.0%
	GTTGAAGGTTCCAACAACAT+CGG	-	chr6.3:37113832-37113851	MS.gene80501:CDS	40.0%
!!	CTCTCTTGCTTTTCTTCCTT+GGG	+	chr6.3:37113883-37113902	None:intergenic	40.0%
!!	TCTCTTGCTTTTCTTCCTTG+GGG	+	chr6.3:37113882-37113901	None:intergenic	40.0%
	CTGATGGAGAAACAATAGGG+TGG	-	chr6.3:37113726-37113745	MS.gene80501:CDS	45.0%
	GATCTTGGTCACAAGTCTGA+TGG	-	chr6.3:37113710-37113729	MS.gene80501:CDS	45.0%
	GGAACCACAAAAGACAGTGT+CGG	-	chr6.3:37113583-37113602	MS.gene80501:CDS	45.0%
	TGTCAGCAACATGTGCATCT+AGG	-	chr6.3:37113669-37113688	MS.gene80501:CDS	45.0%
	TTCCAGTTGACGAGAGATCT+TGG	-	chr6.3:37113695-37113714	MS.gene80501:CDS	45.0%
	TTGCTGACAGTGTGACTCTT+CGG	+	chr6.3:37113658-37113677	None:intergenic	45.0%
!	ACTGTCTTTTGTGGTTCCAG+CGG	+	chr6.3:37113581-37113600	None:intergenic	45.0%
!	TTTTCTTCCTTGGGGCCTTT+TGG	+	chr6.3:37113874-37113893	None:intergenic	45.0%
!!	GCTCTCTTGCTTTTCTTCCT+TGG	+	chr6.3:37113884-37113903	None:intergenic	45.0%
	AAAACAGACGTTGAACCCGC+TGG	-	chr6.3:37113562-37113581	MS.gene80501:CDS	50.0%
	ACATCGGCAGCTAGACCAAA+CGG	-	chr6.3:37113848-37113867	MS.gene80501:CDS	50.0%
	AGCTAGACCAAACGGCCAAA+AGG	-	chr6.3:37113856-37113875	MS.gene80501:CDS	50.0%
	ATCAGAAGACCGTCACACGA+AGG	-	chr6.3:37113619-37113638	MS.gene80501:CDS	50.0%
	GACCAAGATCTCTCGTCAAC+TGG	+	chr6.3:37113700-37113719	None:intergenic	50.0%
	GGCTATGGCCGGAATTATTC+CGG	+	chr6.3:37113795-37113814	None:intergenic	50.0%
	TTCAGAGGGCGGAAAGTTGA+AGG	-	chr6.3:37113817-37113836	MS.gene80501:CDS	50.0%
	TTCGTGTGACGGTCTTCTGA+TGG	+	chr6.3:37113620-37113639	None:intergenic	50.0%
!	CAACTTTCCGCCCTCTGAAA+TGG	+	chr6.3:37113816-37113835	None:intergenic	50.0%
!	CTGTCTTTTGTGGTTCCAGC+GGG	+	chr6.3:37113580-37113599	None:intergenic	50.0%
	AGAAGACCGTCACACGAAGG+TGG	-	chr6.3:37113622-37113641	MS.gene80501:CDS	55.0%
	CCGGCCATAGCCATTTCAGA+GGG	-	chr6.3:37113803-37113822	MS.gene80501:CDS	55.0%
	CCTGCAATCATCAACGCCAC+CGG	-	chr6.3:37113767-37113786	MS.gene80501:CDS	55.0%
	GCCATAGCCATTTCAGAGGG+CGG	-	chr6.3:37113806-37113825	MS.gene80501:CDS	55.0%
	GGTCTAGCTGCCGATGTTGT+TGG	+	chr6.3:37113845-37113864	None:intergenic	55.0%
	TCCGGCCATAGCCATTTCAG+AGG	-	chr6.3:37113802-37113821	MS.gene80501:CDS	55.0%
!	CACCGGCACCGGAATAATTC+CGG	-	chr6.3:37113784-37113803	MS.gene80501:CDS	55.0%
!	TCGGCCGACACTGTCTTTTG+TGG	+	chr6.3:37113590-37113609	None:intergenic	55.0%
!!	CCCTCTGAAATGGCTATGGC+CGG	+	chr6.3:37113806-37113825	None:intergenic	55.0%
!!	TCCGCCCTCTGAAATGGCTA+TGG	+	chr6.3:37113810-37113829	None:intergenic	55.0%
	AGACCGTCACACGAAGGTGG+AGG	-	chr6.3:37113625-37113644	MS.gene80501:CDS	60.0%
	ATCATCAACGCCACCGGCAC+CGG	-	chr6.3:37113773-37113792	MS.gene80501:CDS	60.0%
	CAAACGGCCAAAAGGCCCCA+AGG	-	chr6.3:37113864-37113883	MS.gene80501:CDS	60.0%
	GACCGTCACACGAAGGTGGA+GGG	-	chr6.3:37113626-37113645	MS.gene80501:CDS	60.0%
	GGCCGGAATTATTCCGGTGC+CGG	+	chr6.3:37113789-37113808	None:intergenic	60.0%
	GTCCCTCCACCTTCGTGTGA+CGG	+	chr6.3:37113631-37113650	None:intergenic	60.0%
!	CGGAATTATTCCGGTGCCGG+TGG	+	chr6.3:37113786-37113805	None:intergenic	60.0%
!	CTTGGGGCCTTTTGGCCGTT+TGG	+	chr6.3:37113866-37113885	None:intergenic	60.0%
!!	CCGGTGGCGTTGATGATTGC+AGG	+	chr6.3:37113770-37113789	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	37113557	37113916	37113557	ID=MS.gene80501
chr6.3	mRNA	37113557	37113916	37113557	ID=MS.gene80501.t1;Parent=MS.gene80501
chr6.3	exon	37113557	37113916	37113557	ID=MS.gene80501.t1.exon1;Parent=MS.gene80501.t1
chr6.3	CDS	37113557	37113916	37113557	ID=cds.MS.gene80501.t1;Parent=MS.gene80501.t1

Gene Sequence	>MS.gene80501 ATGGAAAAACAGACGTTGAACCCGCTGGAACCACAAAAGACAGTGTCGGCCGAAAAGAAACCATCAGAAGACCGTCACACGAAGGTGGAGGGACGTGTCCGAAGAGTCACACTGTCAGCAACATGTGCATCTAGGATCTTCCAGTTGACGAGAGATCTTGGTCACAAGTCTGATGGAGAAACAATAGGGTGGTTACTGGAACAAGCTGAACCTGCAATCATCAACGCCACCGGCACCGGAATAATTCCGGCCATAGCCATTTCAGAGGGCGGAAAGTTGAAGGTTCCAACAACATCGGCAGCTAGACCAAACGGCCAAAAGGCCCCAAGGAAGAAAAGCAAGAGAGCCTCAATTGGTTAA
Protein sequence	>MS.gene80501.t1 MEKQTLNPLEPQKTVSAEKKPSEDRHTKVEGRVRRVTLSATCASRIFQLTRDLGHKSDGETIGWLLEQAEPAIINATGTGIIPAIAISEGGKLKVPTTSAARPNGQKAPRKKSKRASIG