AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80504

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80504.t1 MTR_6g043060 72.256 328 62 3 1 299 1 328 7.24e-169 471
MS.gene80504.t1 MTR_8g083100 76.259 278 23 2 23 257 23 300 1.07e-146 421
MS.gene80504.t1 MTR_8g083100 86.364 44 6 0 257 300 414 457 5.54e-18 84.3
MS.gene80504.t1 MTR_2g100680 58.140 301 80 4 1 257 12 310 3.15e-112 332
MS.gene80504.t1 MTR_6g044340 64.062 256 69 2 68 300 8 263 2.18e-110 320
MS.gene80504.t1 MTR_2g100670 53.420 307 118 8 1 298 14 304 7.44e-97 288
MS.gene80504.t1 MTR_2g100670 53.420 307 118 8 1 298 122 412 1.16e-94 286
MS.gene80504.t1 MTR_5g029680 49.763 211 92 4 22 218 32 242 1.60e-63 208
MS.gene80504.t1 MTR_8g087540 48.792 207 96 3 15 216 5 206 1.55e-59 195
MS.gene80504.t1 MTR_6g044430 48.413 252 43 6 100 300 14 229 1.36e-57 185
MS.gene80504.t1 MTR_6g044440 56.983 179 25 3 174 300 1 179 6.12e-56 179
MS.gene80504.t1 MTR_4g077970 44.019 209 105 3 20 216 10 218 2.90e-54 184
MS.gene80504.t1 MTR_1g102860 44.395 223 107 6 7 215 20 239 1.91e-52 175
MS.gene80504.t1 MTR_3g104550 44.118 204 108 3 19 217 43 245 7.65e-51 172
MS.gene80504.t1 MTR_7g095680 46.154 208 100 5 19 215 46 252 9.56e-51 171
MS.gene80504.t1 MTR_1g061170 37.607 234 131 4 20 243 47 275 5.33e-47 164
MS.gene80504.t1 MTR_1g071360 40.724 221 111 4 22 233 32 241 1.05e-46 160
MS.gene80504.t1 MTR_1g071360 40.724 221 111 4 22 233 29 238 1.72e-46 160
MS.gene80504.t1 MTR_3g101870 66.667 102 34 0 12 113 14 115 3.77e-45 157
MS.gene80504.t1 MTR_8g105780 47.179 195 90 6 20 201 20 214 8.23e-45 156
MS.gene80504.t1 MTR_5g017470 61.321 106 41 0 17 122 4 109 1.39e-42 148
MS.gene80504.t1 MTR_5g089170 68.817 93 29 0 21 113 24 116 3.33e-42 146
MS.gene80504.t1 MTR_6g086805 40.196 204 115 2 20 219 9 209 4.82e-42 148
MS.gene80504.t1 MTR_5g010680 59.259 108 44 0 6 113 8 115 1.34e-41 147
MS.gene80504.t1 MTR_2g017890 58.333 108 45 0 12 119 5 112 1.28e-40 145
MS.gene80504.t1 MTR_6g044350 54.795 146 33 3 174 286 1 146 1.79e-39 135
MS.gene80504.t1 MTR_0020s0020 62.366 93 35 0 21 113 32 124 2.25e-38 138
MS.gene80504.t1 MTR_7g091370 59.140 93 38 0 21 113 21 113 3.24e-38 135
MS.gene80504.t1 MTR_1g100777 60.000 95 38 0 21 115 17 111 1.40e-37 135
MS.gene80504.t1 MTR_4g088900 57.732 97 37 1 21 113 32 128 2.16e-34 124
MS.gene80504.t1 MTR_6g044510 66.197 71 16 1 238 300 18 88 4.28e-24 94.0
MS.gene80504.t1 MTR_5g047190 45.361 97 46 2 30 119 1 97 3.70e-22 89.0
MS.gene80504.t1 MTR_4g045757 67.241 58 19 0 22 79 14 71 3.42e-21 85.9
MS.gene80504.t1 MTR_1g061170 28.497 193 115 6 61 243 1 180 1.61e-13 70.5
MS.gene80504.t1 MTR_6g044500 46.341 82 19 3 205 262 1 81 3.54e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80504.t1 AT4G13980 53.755 253 107 3 1 243 1 253 2.19e-86 266
MS.gene80504.t1 AT4G17750 47.222 216 93 4 22 216 52 267 2.06e-58 195
MS.gene80504.t1 AT5G16820 47.867 211 95 5 20 216 25 234 8.32e-58 193
MS.gene80504.t1 AT5G16820 47.867 211 95 5 20 216 25 234 8.32e-58 193
MS.gene80504.t1 AT1G32330 45.532 235 109 5 1 216 16 250 9.65e-58 192
MS.gene80504.t1 AT1G32330 45.532 235 109 5 1 216 16 250 9.86e-58 192
MS.gene80504.t1 AT3G02990 42.489 233 121 4 8 228 9 240 1.36e-57 192
MS.gene80504.t1 AT5G45710 39.048 315 154 9 14 299 5 310 1.45e-55 183
MS.gene80504.t1 AT5G45710 39.048 315 154 9 14 299 5 310 1.45e-55 183
MS.gene80504.t1 AT4G18880 48.598 214 94 4 16 220 9 215 3.65e-52 176
MS.gene80504.t1 AT2G26150 41.892 222 121 4 19 232 41 262 4.23e-50 169
MS.gene80504.t1 AT2G26150 41.892 222 121 4 19 232 41 262 4.23e-50 169
MS.gene80504.t1 AT3G51910 42.654 211 104 5 22 226 29 228 3.71e-48 162
MS.gene80504.t1 AT3G22830 40.741 216 115 3 19 221 58 273 7.13e-48 165
MS.gene80504.t1 AT5G03720 41.584 202 110 3 20 213 53 254 1.07e-45 159
MS.gene80504.t1 AT5G03720 41.584 202 110 3 20 213 53 254 1.07e-45 159
MS.gene80504.t1 AT1G67970 45.312 192 92 5 20 201 17 205 1.11e-44 156
MS.gene80504.t1 AT1G46264 62.500 112 32 1 21 122 33 144 1.32e-44 155
MS.gene80504.t1 AT3G24520 45.087 173 75 3 20 191 15 168 1.98e-44 154
MS.gene80504.t1 AT5G43840 42.246 187 96 3 21 205 18 194 1.93e-42 147
MS.gene80504.t1 AT5G54070 43.169 183 98 2 20 201 69 246 2.08e-41 146
MS.gene80504.t1 AT3G63350 32.509 283 157 8 19 297 25 277 1.02e-40 143
MS.gene80504.t1 AT4G11660 61.000 100 39 0 14 113 51 150 8.16e-39 140
MS.gene80504.t1 AT5G62020 50.420 119 58 1 1 118 1 119 4.71e-38 136
MS.gene80504.t1 AT4G36990 57.407 108 44 1 8 115 2 107 4.84e-38 136
MS.gene80504.t1 AT2G41690 60.000 95 38 0 22 116 40 134 3.09e-37 133
MS.gene80504.t1 AT4G18870 46.591 88 47 0 22 109 146 233 6.73e-23 96.3
MS.gene80504.t1 AT4G18870 45.833 96 51 1 14 109 5 99 1.25e-22 95.5
MS.gene80504.t1 AT1G77570 42.391 92 49 3 19 106 22 113 7.48e-17 76.3
MS.gene80504.t1 AT2G26150 31.000 200 123 6 43 232 13 207 1.87e-15 75.5
MS.gene80504.t1 AT2G26150 31.000 200 123 6 43 232 13 207 1.87e-15 75.5
MS.gene80504.t1 AT5G45710 30.493 223 117 10 106 299 1 214 2.06e-13 68.9
MS.gene80504.t1 AT5G45710 30.493 223 117 10 106 299 1 214 2.06e-13 68.9
MS.gene80504.t1 AT4G19630 34.021 97 61 1 19 112 8 104 3.37e-13 65.9

Find 70 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCAATGCTGCCCTTTC+TGG -0.010683 6.3:+37053939 None:intergenic
CTTCTTTGAGAAGGCTCTTC+AGG 0.202772 6.3:-37053772 MS.gene80504:CDS
ATAATTATAGCTTAGGAGTT+TGG 0.257010 6.3:+37053875 None:intergenic
GAAATTGCTGTGTTTGAAAT+AGG 0.280393 6.3:+37055484 None:intergenic
CCACAGATGAGATCGTTTCT+TGG 0.319260 6.3:-37055571 MS.gene80504:CDS
CAAAATCGAATCCATGGATT+TGG 0.330894 6.3:-37053721 MS.gene80504:CDS
ACGGAGTGAATGATGTGTTT+TGG 0.356521 6.3:-37053500 MS.gene80504:CDS
ATAACAAAAGCTTCATCGTT+TGG 0.362747 6.3:-37055541 MS.gene80504:CDS
AGAAGAACGCGAGAGAATTC+AGG 0.373908 6.3:+37055512 None:intergenic
ACGTTACCTGAAGCCTCCTT+TGG 0.398346 6.3:+37053546 None:intergenic
TAAGAAAAGGCGATTGTCTC+AGG 0.414889 6.3:-37053688 MS.gene80504:CDS
ACCAAGCTACCTTCGGCAAC+AGG 0.417714 6.3:+37053651 None:intergenic
AGCTTAGGAGTTTGGATTGA+AGG 0.419735 6.3:+37053883 None:intergenic
TGCTGTGTTTGAAATAGGTT+GGG 0.448167 6.3:+37055489 None:intergenic
TATCAAGATAATTATAGCTT+AGG 0.451761 6.3:+37053868 None:intergenic
TTGCTGTGTTTGAAATAGGT+TGG 0.457272 6.3:+37055488 None:intergenic
TGGAAACTTATAATAAGAAA+AGG 0.461564 6.3:-37053701 MS.gene80504:CDS
TCTGCTTCCGTCGGCAGCAG+TGG 0.466428 6.3:+37055646 None:intergenic
CCACAGTCATAGTCATCCTC+CGG 0.469987 6.3:-37053976 MS.gene80504:CDS
GCCGAGCAGCTGCTTCGACT+TGG 0.471786 6.3:+37053523 None:intergenic
GCCTGTTGCCGAAGGTAGCT+TGG 0.473073 6.3:-37053652 MS.gene80504:CDS
GAAAGCAGATCCTGATCGTT+GGG 0.475772 6.3:-37054069 MS.gene80504:CDS
TTGGCAAACTCCCAACGATC+AGG 0.478885 6.3:+37054059 None:intergenic
GCTTAGGAGTTTGGATTGAA+GGG 0.480417 6.3:+37053884 None:intergenic
AGATCCAGAAAGGGCAGCAT+TGG 0.480739 6.3:-37053943 MS.gene80504:CDS
ATTATAAGTTTCCAAATCCA+TGG 0.481800 6.3:+37053710 None:intergenic
GCTGATCCAAAGGAGGCTTC+AGG 0.482903 6.3:-37053552 MS.gene80504:CDS
GAAGCCTCCTTTGGATCAGC+GGG 0.484852 6.3:+37053555 None:intergenic
TTTGCAAAACTTCTTTGAGA+AGG 0.487416 6.3:-37053781 MS.gene80504:CDS
TGATACAGAAAAGCTTCAGC+TGG 0.497298 6.3:-37053850 MS.gene80504:CDS
CGACGGAAGCAGAGAAGGTA+TGG 0.499479 6.3:-37055636 MS.gene80504:CDS
ATTGAGCTGACGGATGAAGC+TGG 0.506271 6.3:+37055460 None:intergenic
TTCTCTCAGAATCTTTCTGA+AGG 0.510971 6.3:-37053609 MS.gene80504:CDS
TTCCCGCAAAATCGAATCCA+TGG 0.512391 6.3:-37053727 MS.gene80504:CDS
AACTGACCCCGCTGATCCAA+AGG 0.526560 6.3:-37053562 MS.gene80504:CDS
CTTTCTGAAGGAGAACTGAC+AGG 0.534056 6.3:-37053597 MS.gene80504:CDS
TTCTGCTTTAGATCCAGAAA+GGG 0.539717 6.3:-37053952 MS.gene80504:CDS
GAGAGAAAACATTGCTAGGA+AGG 0.540423 6.3:+37053625 None:intergenic
AACAGCATGGACTTTCAGTA+AGG 0.541453 6.3:+37053469 None:intergenic
TCATACGTCTTCTGAAGAAA+CGG 0.543831 6.3:+37055611 None:intergenic
GGAAAGCAGATCCTGATCGT+TGG 0.545857 6.3:-37054070 MS.gene80504:CDS
GTCGAAGCAGCTGCTCGGCA+CGG 0.548207 6.3:-37053519 MS.gene80504:CDS
TCAGCGGCGATTGGATGGCA+TGG 0.549084 6.3:-37053820 MS.gene80504:CDS
TCCAAGTCGAAGCAGCTGCT+CGG 0.549998 6.3:-37053524 MS.gene80504:CDS
CCGGAGGATGACTATGACTG+TGG 0.551542 6.3:+37053976 None:intergenic
GTTCTGCTTTAGATCCAGAA+AGG 0.553189 6.3:-37053953 MS.gene80504:CDS
AAGGTCGACCAAGCTACCTT+CGG 0.554405 6.3:+37053644 None:intergenic
CACAGTCATAGTCATCCTCC+GGG 0.559092 6.3:-37053975 MS.gene80504:CDS
TTCTGAGAGAAAACATTGCT+AGG 0.570976 6.3:+37053621 None:intergenic
GACTGTGGATAGGTTTCTTG+CGG 0.574041 6.3:+37053991 None:intergenic
GCTGCCGACGGAAGCAGAGA+AGG 0.581055 6.3:-37055641 MS.gene80504:CDS
AGAAACGATCTCATCTGTGG+TGG 0.582151 6.3:+37055574 None:intergenic
TGAAGCCTCCTTTGGATCAG+CGG 0.591974 6.3:+37053554 None:intergenic
CACATGCAGCCTGTTGCCGA+AGG 0.596050 6.3:-37053660 MS.gene80504:CDS
TTACATAGGTATTGAGCTGA+CGG 0.598207 6.3:+37055450 None:intergenic
TCATCAGCCACTGCTGCCGA+CGG 0.606067 6.3:-37055653 MS.gene80504:CDS
GGATGACTATGACTGTGGAT+AGG 0.617132 6.3:+37053981 None:intergenic
TCTTCATTGTCTGAACAGCA+TGG 0.622573 6.3:+37053456 None:intergenic
CATACCTTCTCTGCTTCCGT+CGG 0.623107 6.3:+37055637 None:intergenic
GAAACGATCTCATCTGTGGT+GGG 0.626600 6.3:+37055575 None:intergenic
AGAACGCGAGAGAATTCAGG+AGG 0.628084 6.3:+37055515 None:intergenic
CCAAGAAACGATCTCATCTG+TGG 0.633047 6.3:+37055571 None:intergenic
ATGCTGTTCAGACAATGAAG+AGG 0.637659 6.3:-37053454 MS.gene80504:CDS
TGACCCCGCTGATCCAAAGG+AGG 0.662370 6.3:-37053559 MS.gene80504:CDS
GAACGCGAGAGAATTCAGGA+GGG 0.662504 6.3:+37055516 None:intergenic
TCTTCAGAAGACGTATGACA+TGG 0.665030 6.3:-37055606 MS.gene80504:CDS
TCTGGATCTAAAGCAGAACC+CGG 0.668961 6.3:+37053957 None:intergenic
GCAGTGGCTGATGATGATTG+TGG 0.706595 6.3:+37055662 None:intergenic
AAGCCTCCTTTGGATCAGCG+GGG 0.731334 6.3:+37053556 None:intergenic
GGATCTAAAGCAGAACCCGG+AGG 0.749915 6.3:+37053960 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTCTTGTAAAAATTTAA+TGG - chr6.3:37054886-37054905 MS.gene80504:intron 10.0%
!!! ATCTTTTATAAATTTTTCAT+TGG + chr6.3:37055077-37055096 None:intergenic 10.0%
!! TTATCATATTTATATAGTTC+AGG + chr6.3:37054349-37054368 None:intergenic 15.0%
!!! AAGTATGTTTTAATTTGTTA+TGG - chr6.3:37054989-37055008 MS.gene80504:intron 15.0%
!! AATGGGAAAATTTAACATTT+AGG + chr6.3:37054646-37054665 None:intergenic 20.0%
!! ACTAAATATTATGTCATTGT+CGG + chr6.3:37054079-37054098 None:intergenic 20.0%
!! AGGATTAAACAATAAAAATC+AGG + chr6.3:37054685-37054704 None:intergenic 20.0%
!! AGGTAATATTTGTATAACTA+TGG + chr6.3:37054578-37054597 None:intergenic 20.0%
!! ATAATTTCATTGAGTTTAGT+TGG - chr6.3:37053925-37053944 MS.gene80504:CDS 20.0%
!! ATATTCATAGACTAAATTGA+TGG + chr6.3:37054305-37054324 None:intergenic 20.0%
!! ATGGGAAAATTTAACATTTA+GGG + chr6.3:37054645-37054664 None:intergenic 20.0%
!! ATGTGAAAGATTTGAAAATA+AGG + chr6.3:37054598-37054617 None:intergenic 20.0%
!! GGATTAAACAATAAAAATCA+GGG + chr6.3:37054684-37054703 None:intergenic 20.0%
!! GTATTCTTGTAAAGATTTAA+TGG - chr6.3:37054908-37054927 MS.gene80504:intron 20.0%
!! TATCAAGATAATTATAGCTT+AGG + chr6.3:37055249-37055268 None:intergenic 20.0%
!! TGATTAGTAAGATAAGAATT+AGG - chr6.3:37053885-37053904 MS.gene80504:CDS 20.0%
!! TGGAAACTTATAATAAGAAA+AGG - chr6.3:37055413-37055432 MS.gene80504:intron 20.0%
!! TTTAGTATAATCATATGTGT+CGG - chr6.3:37054093-37054112 MS.gene80504:intron 20.0%
!!! ACTATTTATCATGTTTCTAT+AGG - chr6.3:37054490-37054509 MS.gene80504:intron 20.0%
!!! ATTTATGTGTGCTTTTTATT+AGG - chr6.3:37055015-37055034 MS.gene80504:intron 20.0%
!!! CTATTTATCATGTTTCTATA+GGG - chr6.3:37054491-37054510 MS.gene80504:intron 20.0%
!!! TTATTTTCAAATCTTTCACA+TGG - chr6.3:37054597-37054616 MS.gene80504:intron 20.0%
!!! TTTATGTGTGCTTTTTATTA+GGG - chr6.3:37055016-37055035 MS.gene80504:intron 20.0%
!!! TTTGTTGTATTTGTATGATT+TGG - chr6.3:37053779-37053798 MS.gene80504:CDS 20.0%
! AAATAAGTTTGCAGTTCTTA+TGG - chr6.3:37054941-37054960 MS.gene80504:intron 25.0%
! AAATAGAAATCATGCACTTA+TGG - chr6.3:37054797-37054816 MS.gene80504:intron 25.0%
! ATAATTATAGCTTAGGAGTT+TGG + chr6.3:37055242-37055261 None:intergenic 25.0%
! ATTACACACATAAAAACTTC+AGG + chr6.3:37054861-37054880 None:intergenic 25.0%
! ATTAGGTACAACTTTAGATT+TGG - chr6.3:37053902-37053921 MS.gene80504:CDS 25.0%
! ATTATAAGTTTCCAAATCCA+TGG + chr6.3:37055407-37055426 None:intergenic 25.0%
! TATGAATATCACACTATTTC+CGG - chr6.3:37054316-37054335 MS.gene80504:intron 25.0%
AACAATAAAAATCAGGGTGT+TGG + chr6.3:37054678-37054697 None:intergenic 30.0%
AGATCAAAAACATCTTCTGA+CGG - chr6.3:37055093-37055112 MS.gene80504:intron 30.0%
ATAACAAAAGCTTCATCGTT+TGG - chr6.3:37053573-37053592 MS.gene80504:CDS 30.0%
ATATAGTTCAGGAACTAAAC+CGG + chr6.3:37054338-37054357 None:intergenic 30.0%
ATGGCAATGAGTAAAAACTA+AGG - chr6.3:37054816-37054835 MS.gene80504:intron 30.0%
CTTCATCGAAATTCAATTAG+TGG - chr6.3:37053813-37053832 MS.gene80504:CDS 30.0%
! ATGACTAACATGGGAAATTT+TGG + chr6.3:37054545-37054564 None:intergenic 30.0%
! GAAATTGCTGTGTTTGAAAT+AGG + chr6.3:37053633-37053652 None:intergenic 30.0%
! TAGCATGAATTGACTTACAT+AGG + chr6.3:37053681-37053700 None:intergenic 30.0%
! TATCATGTTTCTATAGGGTA+GGG - chr6.3:37054496-37054515 MS.gene80504:intron 30.0%
! TGACTAACATGGGAAATTTT+GGG + chr6.3:37054544-37054563 None:intergenic 30.0%
! TTATCATGTTTCTATAGGGT+AGG - chr6.3:37054495-37054514 MS.gene80504:intron 30.0%
! TTTGCAAAACTTCTTTGAGA+AGG - chr6.3:37055333-37055352 MS.gene80504:intron 30.0%
!! GTGCTTTTTATTAGGGATTT+CGG - chr6.3:37055023-37055042 MS.gene80504:intron 30.0%
AAAACTTCAGGAAGCAAATC+TGG + chr6.3:37054849-37054868 None:intergenic 35.0%
AAATTAAGACCTACGAAGCA+AGG + chr6.3:37054161-37054180 None:intergenic 35.0%
AGACACATCATGACTAACAT+GGG + chr6.3:37054554-37054573 None:intergenic 35.0%
CAAAATCGAATCCATGGATT+TGG - chr6.3:37055393-37055412 MS.gene80504:intron 35.0%
GACATGAGAATGTTGAAAGA+TGG - chr6.3:37053964-37053983 MS.gene80504:CDS 35.0%
TGACGCATTTCATATACTTC+AGG + chr6.3:37054378-37054397 None:intergenic 35.0%
TTCATTGTTTGTGTCGTGTT+TGG + chr6.3:37054047-37054066 None:intergenic 35.0%
TTCTCTCAGAATCTTTCTGA+AGG - chr6.3:37055505-37055524 MS.gene80504:CDS 35.0%
! AAGAATATTTCTGCAGCATC+TGG - chr6.3:37054242-37054261 MS.gene80504:intron 35.0%
! AATCCATGGATTCGATTTTG+CGG + chr6.3:37055393-37055412 None:intergenic 35.0%
! ATAATCATATGTGTCGGTGT+CGG - chr6.3:37054099-37054118 MS.gene80504:intron 35.0%
! GCTTCGTAGGTCTTAATTTT+CGG - chr6.3:37054162-37054181 MS.gene80504:intron 35.0%
! GTAGGGACTAAATTTGAGAT+AGG - chr6.3:37054513-37054532 MS.gene80504:intron 35.0%
! TCATACGTCTTCTGAAGAAA+CGG + chr6.3:37053506-37053525 None:intergenic 35.0%
! TGCTGTGTTTGAAATAGGTT+GGG + chr6.3:37053628-37053647 None:intergenic 35.0%
! TTCTGAGAGAAAACATTGCT+AGG + chr6.3:37055496-37055515 None:intergenic 35.0%
! TTCTGCTTTAGATCCAGAAA+GGG - chr6.3:37055162-37055181 MS.gene80504:intron 35.0%
! TTGCTGTGTTTGAAATAGGT+TGG + chr6.3:37053629-37053648 None:intergenic 35.0%
!! TTACATAGGTATTGAGCTGA+CGG + chr6.3:37053667-37053686 None:intergenic 35.0%
AACAGCATGGACTTTCAGTA+AGG + chr6.3:37055648-37055667 None:intergenic 40.0%
AGCTTAGGAGTTTGGATTGA+AGG + chr6.3:37055234-37055253 None:intergenic 40.0%
ATGCTGTTCAGACAATGAAG+AGG - chr6.3:37055660-37055679 MS.gene80504:CDS 40.0%
CAGACACATCATGACTAACA+TGG + chr6.3:37054555-37054574 None:intergenic 40.0%
CTACGATGTAGAAGAGAAGT+AGG + chr6.3:37054705-37054724 None:intergenic 40.0%
CTTTCACATGGCAAACCTAT+GGG - chr6.3:37054609-37054628 MS.gene80504:intron 40.0%
GAGAGAAAACATTGCTAGGA+AGG + chr6.3:37055492-37055511 None:intergenic 40.0%
GCTTAGGAGTTTGGATTGAA+GGG + chr6.3:37055233-37055252 None:intergenic 40.0%
TAAGAAAAGGCGATTGTCTC+AGG - chr6.3:37055426-37055445 MS.gene80504:intron 40.0%
TCTTCAGAAGACGTATGACA+TGG - chr6.3:37053508-37053527 MS.gene80504:CDS 40.0%
TCTTCATTGTCTGAACAGCA+TGG + chr6.3:37055661-37055680 None:intergenic 40.0%
TCTTTCACATGGCAAACCTA+TGG - chr6.3:37054608-37054627 MS.gene80504:intron 40.0%
TGATACAGAAAAGCTTCAGC+TGG - chr6.3:37055264-37055283 MS.gene80504:intron 40.0%
! ACGGAGTGAATGATGTGTTT+TGG - chr6.3:37055614-37055633 MS.gene80504:CDS 40.0%
! ATCCATGGATTCGATTTTGC+GGG + chr6.3:37055392-37055411 None:intergenic 40.0%
! CGGAGTGAATGATGTGTTTT+GGG - chr6.3:37055615-37055634 MS.gene80504:CDS 40.0%
! GTTCTGCTTTAGATCCAGAA+AGG - chr6.3:37055161-37055180 MS.gene80504:intron 40.0%
! TGTGAATTGGTGGGTGATTT+AGG - chr6.3:37054191-37054210 MS.gene80504:intron 40.0%
! TTCAGCTGGAAGATTTTCAG+CGG - chr6.3:37055278-37055297 MS.gene80504:intron 40.0%
!! GGTGTTGGTATGCACTTAAT+GGG + chr6.3:37054663-37054682 None:intergenic 40.0%
ACTTCAGGAAGCAAATCTGG+TGG + chr6.3:37054846-37054865 None:intergenic 45.0%
AGAAACGATCTCATCTGTGG+TGG + chr6.3:37053543-37053562 None:intergenic 45.0%
AGAAGAACGCGAGAGAATTC+AGG + chr6.3:37053605-37053624 None:intergenic 45.0%
CCAAGAAACGATCTCATCTG+TGG + chr6.3:37053546-37053565 None:intergenic 45.0%
CCACAGATGAGATCGTTTCT+TGG - chr6.3:37053543-37053562 MS.gene80504:CDS 45.0%
CGTAGACATCTCAAGCATCT+AGG - chr6.3:37054720-37054739 MS.gene80504:intron 45.0%
GAAACGATCTCATCTGTGGT+GGG + chr6.3:37053542-37053561 None:intergenic 45.0%
GAAAGCAGATCCTGATCGTT+GGG - chr6.3:37055045-37055064 MS.gene80504:intron 45.0%
GACTGTGGATAGGTTTCTTG+CGG + chr6.3:37055126-37055145 None:intergenic 45.0%
GGATGACTATGACTGTGGAT+AGG + chr6.3:37055136-37055155 None:intergenic 45.0%
TAGGGTTCCAAAACACCCAT+AGG + chr6.3:37054627-37054646 None:intergenic 45.0%
TTCCCGCAAAATCGAATCCA+TGG - chr6.3:37055387-37055406 MS.gene80504:intron 45.0%
TTTCTGCAGCATCTGGATCT+TGG - chr6.3:37054249-37054268 MS.gene80504:intron 45.0%
! CTTCTTTGAGAAGGCTCTTC+AGG - chr6.3:37055342-37055361 MS.gene80504:intron 45.0%
! CTTTCTGAAGGAGAACTGAC+AGG - chr6.3:37055517-37055536 MS.gene80504:CDS 45.0%
! TCTGGATCTAAAGCAGAACC+CGG + chr6.3:37055160-37055179 None:intergenic 45.0%
! TGGCAAACCTATGGGTGTTT+TGG - chr6.3:37054617-37054636 MS.gene80504:intron 45.0%
!! GGGTGTTGGTATGCACTTAA+TGG + chr6.3:37054664-37054683 None:intergenic 45.0%
!! TTTTCGGTGTGCGTGTGAAT+TGG - chr6.3:37054178-37054197 MS.gene80504:intron 45.0%
AAGGTCGACCAAGCTACCTT+CGG + chr6.3:37055473-37055492 None:intergenic 50.0%
ACGTTACCTGAAGCCTCCTT+TGG + chr6.3:37055571-37055590 None:intergenic 50.0%
AGAACGCGAGAGAATTCAGG+AGG + chr6.3:37053602-37053621 None:intergenic 50.0%
AGATCCAGAAAGGGCAGCAT+TGG - chr6.3:37055171-37055190 MS.gene80504:intron 50.0%
CACAGTCATAGTCATCCTCC+GGG - chr6.3:37055139-37055158 MS.gene80504:intron 50.0%
CAGGAAGCAAATCTGGTGGT+GGG + chr6.3:37054842-37054861 None:intergenic 50.0%
CATACCTTCTCTGCTTCCGT+CGG + chr6.3:37053480-37053499 None:intergenic 50.0%
CCACAGTCATAGTCATCCTC+CGG - chr6.3:37055138-37055157 MS.gene80504:intron 50.0%
GAACGCGAGAGAATTCAGGA+GGG + chr6.3:37053601-37053620 None:intergenic 50.0%
GGAAAGCAGATCCTGATCGT+TGG - chr6.3:37055044-37055063 MS.gene80504:intron 50.0%
TACGAAGCAAGGACACTCCT+CGG + chr6.3:37054150-37054169 None:intergenic 50.0%
TCAGGAAGCAAATCTGGTGG+TGG + chr6.3:37054843-37054862 None:intergenic 50.0%
TGAAGCCTCCTTTGGATCAG+CGG + chr6.3:37055563-37055582 None:intergenic 50.0%
TTCTCCAATGCTGCCCTTTC+TGG + chr6.3:37055178-37055197 None:intergenic 50.0%
TTGGCAAACTCCCAACGATC+AGG + chr6.3:37055058-37055077 None:intergenic 50.0%
! ATTGAGCTGACGGATGAAGC+TGG + chr6.3:37053657-37053676 None:intergenic 50.0%
! GATTTTCAGCGGCGATTGGA+TGG - chr6.3:37055289-37055308 MS.gene80504:intron 50.0%
! GCAGTGGCTGATGATGATTG+TGG + chr6.3:37053455-37053474 None:intergenic 50.0%
! GGAAGATTTTCAGCGGCGAT+TGG - chr6.3:37055285-37055304 MS.gene80504:intron 50.0%
AACTGACCCCGCTGATCCAA+AGG - chr6.3:37055552-37055571 MS.gene80504:CDS 55.0%
AAGCCTCCTTTGGATCAGCG+GGG + chr6.3:37055561-37055580 None:intergenic 55.0%
ACCAAGCTACCTTCGGCAAC+AGG + chr6.3:37055466-37055485 None:intergenic 55.0%
CCGGAGGATGACTATGACTG+TGG + chr6.3:37055141-37055160 None:intergenic 55.0%
CGACGGAAGCAGAGAAGGTA+TGG - chr6.3:37053478-37053497 MS.gene80504:CDS 55.0%
CGGTGTGCGTGTGAATTGGT+GGG - chr6.3:37054182-37054201 MS.gene80504:intron 55.0%
GAAGCCTCCTTTGGATCAGC+GGG + chr6.3:37055562-37055581 None:intergenic 55.0%
GCAAGGACACTCCTCGGATT+AGG + chr6.3:37054144-37054163 None:intergenic 55.0%
GCTGATCCAAAGGAGGCTTC+AGG - chr6.3:37055562-37055581 MS.gene80504:CDS 55.0%
TCCAAGTCGAAGCAGCTGCT+CGG - chr6.3:37055590-37055609 MS.gene80504:CDS 55.0%
TCGGTGTGCGTGTGAATTGG+TGG - chr6.3:37054181-37054200 MS.gene80504:intron 55.0%
! GGATCTAAAGCAGAACCCGG+AGG + chr6.3:37055157-37055176 None:intergenic 55.0%
!! GAGGAGTGTCCTTGCTTCGT+AGG - chr6.3:37054149-37054168 MS.gene80504:intron 55.0%
CACATGCAGCCTGTTGCCGA+AGG - chr6.3:37055454-37055473 MS.gene80504:CDS 60.0%
GATTAGGTGCGTCCTGGCGT+CGG + chr6.3:37054128-37054147 None:intergenic 60.0%
GCCTGTTGCCGAAGGTAGCT+TGG - chr6.3:37055462-37055481 MS.gene80504:CDS 60.0%
TCAGCGGCGATTGGATGGCA+TGG - chr6.3:37055294-37055313 MS.gene80504:intron 60.0%
TCATCAGCCACTGCTGCCGA+CGG - chr6.3:37053461-37053480 MS.gene80504:CDS 60.0%
TGACCCCGCTGATCCAAAGG+AGG - chr6.3:37055555-37055574 MS.gene80504:CDS 60.0%
! TCCTCGGATTAGGTGCGTCC+TGG + chr6.3:37054134-37054153 None:intergenic 60.0%
GCCAGGACGCACCTAATCCG+AGG - chr6.3:37054130-37054149 MS.gene80504:intron 65.0%
GCCGAGCAGCTGCTTCGACT+TGG + chr6.3:37055594-37055613 None:intergenic 65.0%
GCTGCCGACGGAAGCAGAGA+AGG - chr6.3:37053473-37053492 MS.gene80504:CDS 65.0%
GTCGAAGCAGCTGCTCGGCA+CGG - chr6.3:37055595-37055614 MS.gene80504:CDS 65.0%
TCTGCTTCCGTCGGCAGCAG+TGG + chr6.3:37053471-37053490 None:intergenic 65.0%
! CGGTGTCGGACACCGACGCC+AGG - chr6.3:37054113-37054132 MS.gene80504:intron 75.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 37053440 37055696 37053440 ID=MS.gene80504
chr6.3 mRNA 37053440 37055696 37053440 ID=MS.gene80504.t1;Parent=MS.gene80504
chr6.3 exon 37055454 37055696 37055454 ID=MS.gene80504.t1.exon1;Parent=MS.gene80504.t1
chr6.3 CDS 37055454 37055696 37055454 ID=cds.MS.gene80504.t1;Parent=MS.gene80504.t1
chr6.3 exon 37053440 37054099 37053440 ID=MS.gene80504.t1.exon2;Parent=MS.gene80504.t1
chr6.3 CDS 37053440 37054099 37053440 ID=cds.MS.gene80504.t1;Parent=MS.gene80504.t1
Gene Sequence

>MS.gene80504

ATGAATTCTGCACCACAATCATCATCAGCCACTGCTGCCGACGGAAGCAGAGAAGGTATGGCACCGTTTCTTCAGAAGACGTATGACATGGTAGATGATCCCACCACAGATGAGATCGTTTCTTGGAGCTCTGATAACAAAAGCTTCATCGTTTGGAACCCTCCTGAATTCTCTCGCGTTCTTCTCCCAACCTATTTCAAACACAGCAATTTCTCCAGCTTCATCCGTCAGCTCAATACCTATGTAAGTCAATTCATGCTATAAGCTTCTTTTGATTATTTCATTGTTACTTTGTATGATCAATTAGTTATTGTCTTTCACTGTGTTTTTGCTTGATTGTTTGTTGTATTTGTATGATTTGGTTTCATTGTGTCTTCATCGAAATTCAATTAGTGGATATTGATTATTATGATGATCATGATCATTAAGAGATTGATGAAAAAAATGATTAGTAAGATAAGAATTAGGTACAACTTTAGATTTGGATAATTTCATTGAGTTTAGTTGGAGATACAAAATGTTGAGACATGAGAATGTTGAAAGATGGATGAGATATTATAGTCTTGTATGACACTTTTATTCTATGAAACATAAAACACAGACACCAAACACGACACAAACAATGAAACGCAAACACCGACAATGACATAATATTTAGTATAATCATATGTGTCGGTGTCGGACACCGACGCCAGGACGCACCTAATCCGAGGAGTGTCCTTGCTTCGTAGGTCTTAATTTTCGGTGTGCGTGTGAATTGGTGGGTGATTTAGGACTCTAAATGCACTCAAGAAAAACGATCAAGAATATTTCTGCAGCATCTGGATCTTGGTAATGATGATACATCGAATCATGAATAAAACCATCAATTTAGTCTATGAATATCACACTATTTCCGGTTTAGTTCCTGAACTATATAAATATGATAAGTAGTTCCTGAAGTATATGAAATGCGTCAATTTAATCTTTGAAGTATACTGAAATTCGTCAATTTTGTCTCTAACATCTTAATTGTTGTGACTAAATTTATGAGTTTCATATACTTTAGAGACTATTTATCATGTTTCTATAGGGTAGGGACTAAATTTGAGATAGGATTTACCCAAAATTTCCCATGTTAGTCATGATGTGTCTGCCATAGTTATACAAATATTACCTTATTTTCAAATCTTTCACATGGCAAACCTATGGGTGTTTTGGAACCCTAAATGTTAAATTTTCCCATTAAGTGCATACCAACACCCTGATTTTTATTGTTTAATCCTACTTCTCTTCTACATCGTAGACATCTCAAGCATCTAGGTTTTCATTTATTGAACTTGTTGAAATTGATATCTAAGTCTTAGATATGATTGAAAAATAGAAATCATGCACTTATGGCAATGAGTAAAAACTAAGGCCCACCACCAGATTTGCTTCCTGAAGTTTTTATGTGTGTAATGAAGAATATTCTTGTAAAAATTTAATGGTATTCTTGTAAAGATTTAATGGTAAATCGTTCAAATAAGTTTGCAGTTCTTATGGCTTTAATGATAAATAATTGATTATGAAGTATGTTTTAATTTGTTATGGTTTATTTATGTGTGCTTTTTATTAGGGATTTCGGAAAGCAGATCCTGATCGTTGGGAGTTTGCCAATGAAAAATTTATAAAAGATCAAAAACATCTTCTGACGGATATACACCGCAAGAAACCTATCCACAGTCATAGTCATCCTCCGGGTTCTGCTTTAGATCCAGAAAGGGCAGCATTGGAGAAAGAAATAGAGAAGCTGTTGCGCGAGAAAAATGCCCTTCAATCCAAACTCCTAAGCTATAATTATCTTGATACAGAAAAGCTTCAGCTGGAAGATTTTCAGCGGCGATTGGATGGCATGGAGAAGAAGCAGACAAATTTGCAAAACTTCTTTGAGAAGGCTCTTCAGGATTCTTTTATTGTTGAACTACTTTCCCGCAAAATCGAATCCATGGATTTGGAAACTTATAATAAGAAAAGGCGATTGTCTCAGGTTGATCACATGCAGCCTGTTGCCGAAGGTAGCTTGGTCGACCTTCCTAGCAATGTTTTCTCTCAGAATCTTTCTGAAGGAGAACTGACAGGAATACAGACAAGAACTGACCCCGCTGATCCAAAGGAGGCTTCAGGTAACGTCCAAGTCGAAGCAGCTGCTCGGCACGGAGTGAATGATGTGTTTTGGGAACAATTCCTTACTGAAAGTCCATGCTGTTCAGACAATGAAGAGGCATTGTCCAACTAA
Protein sequence

>MS.gene80504.t1

MNSAPQSSSATAADGSREGMAPFLQKTYDMVDDPTTDEIVSWSSDNKSFIVWNPPEFSRVLLPTYFKHSNFSSFIRQLNTYGFRKADPDRWEFANEKFIKDQKHLLTDIHRKKPIHSHSHPPGSALDPERAALEKEIEKLLREKNALQSKLLSYNYLDTEKLQLEDFQRRLDGMEKKQTNLQNFFEKALQDSFIVELLSRKIESMDLETYNKKRRLSQVDHMQPVAEGSLVDLPSNVFSQNLSEGELTGIQTRTDPADPKEASGNVQVEAAARHGVNDVFWEQFLTESPCCSDNEEALSN