AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80507

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80507.t1 MTR_6g043060 69.452 347 56 4 1 317 1 327 4.33e-165 462
MS.gene80507.t1 MTR_8g083100 75.347 288 38 5 23 277 23 310 2.75e-144 416
MS.gene80507.t1 MTR_8g083100 74.603 63 16 0 257 319 395 457 5.29e-22 96.3
MS.gene80507.t1 MTR_6g044340 63.433 268 70 2 68 319 8 263 1.81e-110 322
MS.gene80507.t1 MTR_2g100680 56.090 312 101 5 1 278 12 321 8.70e-110 327
MS.gene80507.t1 MTR_2g100670 56.522 276 102 5 1 261 14 286 3.97e-95 284
MS.gene80507.t1 MTR_2g100670 56.522 276 102 5 1 261 122 394 3.04e-93 283
MS.gene80507.t1 MTR_5g029680 50.237 211 91 4 22 218 32 242 7.96e-63 207
MS.gene80507.t1 MTR_8g087540 38.177 351 167 7 15 315 5 355 1.09e-62 204
MS.gene80507.t1 MTR_6g044430 50.988 253 46 5 100 315 14 225 1.12e-61 196
MS.gene80507.t1 MTR_6g044440 60.894 179 37 2 174 319 1 179 7.94e-61 192
MS.gene80507.t1 MTR_4g077970 44.019 209 105 3 20 216 10 218 5.12e-53 181
MS.gene80507.t1 MTR_3g104550 34.967 306 185 6 19 317 43 341 4.02e-51 173
MS.gene80507.t1 MTR_1g102860 43.243 222 109 6 7 214 20 238 1.64e-50 171
MS.gene80507.t1 MTR_7g095680 45.631 206 102 4 19 214 46 251 2.98e-50 171
MS.gene80507.t1 MTR_6g044350 60.403 149 39 3 174 305 1 146 2.40e-46 154
MS.gene80507.t1 MTR_1g071360 44.571 175 89 2 22 195 32 199 3.29e-46 159
MS.gene80507.t1 MTR_1g071360 44.571 175 89 2 22 195 29 196 5.25e-46 160
MS.gene80507.t1 MTR_1g061170 40.404 198 115 2 20 214 47 244 1.05e-45 161
MS.gene80507.t1 MTR_8g105780 48.718 195 87 6 20 201 20 214 3.73e-45 157
MS.gene80507.t1 MTR_3g101870 66.667 102 34 0 12 113 14 115 2.54e-44 155
MS.gene80507.t1 MTR_5g017470 62.264 106 40 0 17 122 4 109 3.56e-43 150
MS.gene80507.t1 MTR_6g086805 39.720 214 102 2 20 219 9 209 6.92e-42 148
MS.gene80507.t1 MTR_5g089170 68.817 93 29 0 21 113 24 116 2.72e-41 144
MS.gene80507.t1 MTR_5g010680 59.259 108 44 0 6 113 8 115 4.54e-41 146
MS.gene80507.t1 MTR_2g017890 58.333 108 45 0 12 119 5 112 3.09e-40 145
MS.gene80507.t1 MTR_7g091370 59.140 93 38 0 21 113 21 113 6.58e-38 135
MS.gene80507.t1 MTR_0020s0020 62.366 93 35 0 21 113 32 124 1.10e-37 137
MS.gene80507.t1 MTR_1g100777 60.000 95 38 0 21 115 17 111 5.46e-37 134
MS.gene80507.t1 MTR_4g088900 58.763 97 36 1 21 113 32 128 1.08e-34 125
MS.gene80507.t1 MTR_6g044510 77.632 76 15 1 246 319 13 88 5.39e-32 115
MS.gene80507.t1 MTR_5g047190 45.361 97 46 2 30 119 1 97 1.28e-21 87.8
MS.gene80507.t1 MTR_4g045757 67.241 58 19 0 22 79 14 71 9.21e-21 85.1
MS.gene80507.t1 MTR_6g044500 52.941 68 21 1 205 261 1 68 6.59e-13 63.9
MS.gene80507.t1 MTR_1g061170 29.936 157 99 4 61 214 1 149 1.37e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80507.t1 AT4G13980 53.846 234 106 2 1 232 1 234 7.54e-82 255
MS.gene80507.t1 AT1G32330 49.765 213 92 4 1 198 16 228 1.20e-58 196
MS.gene80507.t1 AT1G32330 49.765 213 92 4 1 198 16 228 1.29e-58 196
MS.gene80507.t1 AT4G17750 47.685 216 92 4 22 216 52 267 1.37e-57 193
MS.gene80507.t1 AT5G16820 48.341 211 94 5 20 216 25 234 6.77e-57 191
MS.gene80507.t1 AT5G16820 48.341 211 94 5 20 216 25 234 6.77e-57 191
MS.gene80507.t1 AT3G02990 42.918 233 120 4 8 228 9 240 1.21e-56 190
MS.gene80507.t1 AT5G45710 35.962 317 174 7 14 315 5 307 3.61e-53 178
MS.gene80507.t1 AT5G45710 35.962 317 174 7 14 315 5 307 3.61e-53 178
MS.gene80507.t1 AT4G18880 47.867 211 100 3 16 220 9 215 3.52e-51 174
MS.gene80507.t1 AT2G26150 42.793 222 119 4 19 232 41 262 2.67e-50 170
MS.gene80507.t1 AT2G26150 42.793 222 119 4 19 232 41 262 2.67e-50 170
MS.gene80507.t1 AT3G51910 43.128 211 103 5 22 226 29 228 9.87e-50 167
MS.gene80507.t1 AT3G22830 41.905 210 108 4 19 214 58 267 3.59e-47 164
MS.gene80507.t1 AT3G24520 46.243 173 73 3 20 191 15 168 1.20e-45 158
MS.gene80507.t1 AT5G03720 41.584 202 110 3 20 213 53 254 2.25e-45 159
MS.gene80507.t1 AT5G03720 41.584 202 110 3 20 213 53 254 2.25e-45 159
MS.gene80507.t1 AT1G67970 45.128 195 94 5 20 204 17 208 2.99e-44 155
MS.gene80507.t1 AT1G46264 62.500 112 32 1 21 122 33 144 7.50e-44 154
MS.gene80507.t1 AT5G43840 42.246 187 96 3 21 205 18 194 1.81e-42 148
MS.gene80507.t1 AT5G54070 42.703 185 100 2 20 203 69 248 2.02e-40 144
MS.gene80507.t1 AT3G63350 34.762 210 136 1 19 227 25 234 2.16e-39 140
MS.gene80507.t1 AT4G11660 61.000 100 39 0 14 113 51 150 2.37e-38 140
MS.gene80507.t1 AT4G36990 57.407 108 44 1 8 115 2 107 9.74e-38 136
MS.gene80507.t1 AT5G62020 50.420 119 58 1 1 118 1 119 1.62e-37 135
MS.gene80507.t1 AT2G41690 51.304 115 56 0 22 136 40 154 5.56e-37 133
MS.gene80507.t1 AT4G18870 46.591 88 47 0 22 109 146 233 2.25e-22 95.1
MS.gene80507.t1 AT4G18870 45.833 96 51 1 14 109 5 99 3.79e-22 94.7
MS.gene80507.t1 AT1G77570 42.391 92 49 3 19 106 22 113 6.62e-17 76.6
MS.gene80507.t1 AT2G26150 32.000 200 121 6 43 232 13 207 1.28e-15 76.3
MS.gene80507.t1 AT2G26150 32.000 200 121 6 43 232 13 207 1.28e-15 76.3
MS.gene80507.t1 AT4G19630 34.021 97 61 1 19 112 8 104 8.32e-14 67.8
MS.gene80507.t1 AT5G45710 25.333 225 139 7 106 315 1 211 2.42e-11 63.5
MS.gene80507.t1 AT5G45710 25.333 225 139 7 106 315 1 211 2.42e-11 63.5

Find 74 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCAATGCTGCCCTTTC+TGG -0.010683 6.3:+36994661 None:intergenic
CTTCTTTGTGAAGGCTCTTC+AGG 0.202681 6.3:-36994494 MS.gene80507:CDS
TGCAGGCTTTCCTCATCTTC+AGG 0.204361 6.3:+36994310 None:intergenic
ATAATTATAGCTTAAGAGTT+TGG 0.284728 6.3:+36994597 None:intergenic
GAAATTGCTGTGTTTGAAAT+AGG 0.299469 6.3:+36995867 None:intergenic
CCACAGATGAGATCGTTTCT+TGG 0.319260 6.3:-36995954 MS.gene80507:CDS
CAAAATCAAATCCATGGATT+TGG 0.330894 6.3:-36994443 MS.gene80507:CDS
CTTTCTGAAGGAGAACTTAC+TGG 0.346936 6.3:-36994280 MS.gene80507:CDS
ACGGAGTGAATGATGTGTTT+TGG 0.356521 6.3:-36994165 MS.gene80507:CDS
ATAACAAAAGCTTCATCGTT+TGG 0.362747 6.3:-36995924 MS.gene80507:CDS
GCAGGCTTTCCTCATCTTCA+GGG 0.373078 6.3:+36994311 None:intergenic
AGAAGAACGCGAGAGAATTC+AGG 0.373908 6.3:+36995895 None:intergenic
ACGTTACCTGAAGCCTCCTT+TGG 0.398346 6.3:+36994211 None:intergenic
ACCAAGCTACCTTCGGCAAC+AGG 0.417304 6.3:+36994373 None:intergenic
AGCTTAAGAGTTTGGATTGA+AGG 0.419735 6.3:+36994605 None:intergenic
TAAGAAGAGGGGATTGTCTC+AGG 0.434641 6.3:-36994410 MS.gene80507:CDS
TTCCCGCAAAATCAAATCCA+TGG 0.455530 6.3:-36994449 MS.gene80507:CDS
GAGTTTATTTGAGATATCTT+CGG 0.461136 6.3:+36994333 None:intergenic
TGGAAACTTATAATAAGAAG+AGG 0.463694 6.3:-36994423 MS.gene80507:CDS
TCTGCTTCCGTCGGCAGCAG+TGG 0.466428 6.3:+36996029 None:intergenic
GCTGATCCAAAGGAGGCTTC+AGG 0.466668 6.3:-36994217 MS.gene80507:CDS
CCACAGTCATAGTCATCCTC+CGG 0.469987 6.3:-36994698 MS.gene80507:CDS
GCCGAGCAGCTGCTTCGACT+TGG 0.471786 6.3:+36994188 None:intergenic
GGAAACTTATAATAAGAAGA+GGG 0.473221 6.3:-36994422 MS.gene80507:CDS
AAAATCAGATCCTGATCGTT+GGG 0.473476 6.3:-36994791 MS.gene80507:CDS
TTGGCAAACTCCCAACGATC+AGG 0.478885 6.3:+36994781 None:intergenic
TTGCTGTGTTTGAAATAGGC+TGG 0.480359 6.3:+36995871 None:intergenic
GCTTAAGAGTTTGGATTGAA+GGG 0.480417 6.3:+36994606 None:intergenic
ATTATAAGTTTCCAAATCCA+TGG 0.481800 6.3:+36994432 None:intergenic
AGATCCAGAAAGGGCAGCAT+TGG 0.494908 6.3:-36994665 MS.gene80507:CDS
CGACGGAAGCAGAGAAGGTA+TGG 0.499479 6.3:-36996019 MS.gene80507:CDS
TGAGACAGAAAAGCTTCAGC+TGG 0.500611 6.3:-36994572 MS.gene80507:CDS
ATTGAGCTGACGGATGAAGC+TGG 0.506271 6.3:+36995843 None:intergenic
GCCTGTTGCCGAAGGTAGCT+TGG 0.510527 6.3:-36994374 MS.gene80507:CDS
ATATCTTCGGCAACATTCCA+TGG 0.514486 6.3:+36994346 None:intergenic
TTTGCAGAACTTCTTTGTGA+AGG 0.519196 6.3:-36994503 MS.gene80507:CDS
TTTCTGAAGGAGAACTTACT+GGG 0.521375 6.3:-36994279 MS.gene80507:CDS
TGGATCAACCAAGCTACCTT+CGG 0.537647 6.3:+36994366 None:intergenic
AACAGCATGGACTTTCAGTA+AGG 0.541453 6.3:+36994134 None:intergenic
GAAAATCAGATCCTGATCGT+TGG 0.543561 6.3:-36994792 MS.gene80507:CDS
GAAACTTATAATAAGAAGAG+GGG 0.544462 6.3:-36994421 MS.gene80507:CDS
GTCGAAGCAGCTGCTCGGCA+CGG 0.548207 6.3:-36994184 MS.gene80507:CDS
TCAGCGGCGATTGGAAGGCA+TGG 0.549084 6.3:-36994542 MS.gene80507:CDS
TCCAAGTCGAAGCAGCTGCT+CGG 0.549998 6.3:-36994189 MS.gene80507:CDS
CCGGAGGATGACTATGACTG+TGG 0.551542 6.3:+36994698 None:intergenic
CACAGTCATAGTCATCCTCC+GGG 0.559092 6.3:-36994697 MS.gene80507:CDS
TTCTGCTGTAGATCCAGAAA+GGG 0.565003 6.3:-36994674 MS.gene80507:CDS
TCATACGTCTTCAGAAGAAA+CGG 0.565669 6.3:+36995994 None:intergenic
GTTCTGCTGTAGATCCAGAA+AGG 0.569845 6.3:-36994675 MS.gene80507:CDS
AAGGTAGCTTGGTTGATCCA+TGG 0.570487 6.3:-36994363 MS.gene80507:CDS
GACTGTGGATAGGTTTCTTG+CGG 0.574041 6.3:+36994713 None:intergenic
TGCTGTGTTTGAAATAGGCT+GGG 0.577118 6.3:+36995872 None:intergenic
AAATAAACTCCCTGAAGATG+AGG 0.580535 6.3:-36994320 MS.gene80507:CDS
GCTGCCGACGGAAGCAGAGA+AGG 0.581055 6.3:-36996024 MS.gene80507:CDS
AGAAACGATCTCATCTGTGG+TGG 0.582151 6.3:+36995957 None:intergenic
GATTGGAAGGCATGGAGAAG+AGG 0.593047 6.3:-36994534 MS.gene80507:CDS
CACATGCAGCCTGTTGCCGA+AGG 0.596050 6.3:-36994382 MS.gene80507:CDS
TCTTCATTGTCTGAACAGCA+TGG 0.597736 6.3:+36994121 None:intergenic
TTACATAGGTATTGAGCTGA+CGG 0.598207 6.3:+36995833 None:intergenic
TCATCAGCCACTGCTGCCGA+CGG 0.606067 6.3:-36996036 MS.gene80507:CDS
TCTGGATCTACAGCAGAACC+CGG 0.614712 6.3:+36994679 None:intergenic
GGATGACTATGACTGTGGAT+AGG 0.617132 6.3:+36994703 None:intergenic
CATACCTTCTCTGCTTCCGT+CGG 0.623107 6.3:+36996020 None:intergenic
GAAACGATCTCATCTGTGGT+GGG 0.626600 6.3:+36995958 None:intergenic
AGAACGCGAGAGAATTCAGG+AGG 0.628084 6.3:+36995898 None:intergenic
CCAAGAAACGATCTCATCTG+TGG 0.633047 6.3:+36995954 None:intergenic
ATGCTGTTCAGACAATGAAG+AGG 0.637659 6.3:-36994119 MS.gene80507:CDS
TATTAAGCTAGCTGATCCAA+AGG 0.648739 6.3:-36994227 MS.gene80507:CDS
TCTTCTGAAGACGTATGACA+TGG 0.660108 6.3:-36995989 MS.gene80507:CDS
GAACGCGAGAGAATTCAGGA+GGG 0.662504 6.3:+36995899 None:intergenic
TAAGCTAGCTGATCCAAAGG+AGG 0.675297 6.3:-36994224 MS.gene80507:CDS
TTCTGAAGGAGAACTTACTG+GGG 0.695684 6.3:-36994278 MS.gene80507:CDS
GCAGTGGCTGATGATGATTG+TGG 0.706595 6.3:+36996045 None:intergenic
GGATCTACAGCAGAACCCGG+AGG 0.744225 6.3:+36994682 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTTTTATAAATTTTTCAT+TGG + chr6.3:36995403-36995422 None:intergenic 10.0%
!! TTATCATATTTATATAGTTC+AGG + chr6.3:36995014-36995033 None:intergenic 15.0%
!!! ATGTATGTTTTAATTTGTTA+CGG - chr6.3:36995315-36995334 MS.gene80507:intron 15.0%
!! AGGATTAAACAATAAAAATC+AGG + chr6.3:36995043-36995062 None:intergenic 20.0%
!! ATAATTATAGCTTAAGAGTT+TGG + chr6.3:36995568-36995587 None:intergenic 20.0%
!! ATATTCTTGTAGAGATTTAA+TGG - chr6.3:36995240-36995259 MS.gene80507:intron 20.0%
!! ATTATTTCATTGAGTTTAGT+TGG - chr6.3:36994590-36994609 MS.gene80507:CDS 20.0%
!! CTTTATTAAACAAAAACTTC+AGG + chr6.3:36995219-36995238 None:intergenic 20.0%
!!! TTTGTTGTATTTGTATGATT+TGG - chr6.3:36994444-36994463 MS.gene80507:CDS 20.0%
! AAATAGAAATCATGCACTTA+TGG - chr6.3:36995155-36995174 MS.gene80507:intron 25.0%
! ATATTCAGAGACTAAATTGA+TGG + chr6.3:36994970-36994989 None:intergenic 25.0%
! ATTAGGTACAACTTTAGATT+TGG - chr6.3:36994567-36994586 MS.gene80507:CDS 25.0%
! ATTATAAGTTTCCAAATCCA+TGG + chr6.3:36995733-36995752 None:intergenic 25.0%
! GAAACTTATAATAAGAAGAG+GGG - chr6.3:36995741-36995760 MS.gene80507:intron 25.0%
! GGAAACTTATAATAAGAAGA+GGG - chr6.3:36995740-36995759 MS.gene80507:intron 25.0%
! TGATATCTAAGTCTTAGATA+GGG - chr6.3:36995127-36995146 MS.gene80507:intron 25.0%
! TGATTAGCAAGATAAGAATT+AGG - chr6.3:36994550-36994569 MS.gene80507:CDS 25.0%
! TGGAAACTTATAATAAGAAG+AGG - chr6.3:36995739-36995758 MS.gene80507:intron 25.0%
! TTCAGTATAATCATATGTGT+CGG - chr6.3:36994756-36994775 MS.gene80507:CDS 25.0%
! TTGATATCTAAGTCTTAGAT+AGG - chr6.3:36995126-36995145 MS.gene80507:intron 25.0%
!! GAGTTTATTTGAGATATCTT+CGG + chr6.3:36995832-36995851 None:intergenic 25.0%
!!! TGAGATATTATAGTCTTGTA+TGG - chr6.3:36994653-36994672 MS.gene80507:CDS 25.0%
AGATCAAAAACATCTTCTGA+CGG - chr6.3:36995419-36995438 MS.gene80507:intron 30.0%
ATAACAAAAGCTTCATCGTT+TGG - chr6.3:36994238-36994257 MS.gene80507:CDS 30.0%
ATATAGTTCAGGAACTAAAC+CGG + chr6.3:36995003-36995022 None:intergenic 30.0%
ATCGTTCAAAATAGCTCTTA+TGG - chr6.3:36995266-36995285 MS.gene80507:intron 30.0%
CAAAATCAAATCCATGGATT+TGG - chr6.3:36995719-36995738 MS.gene80507:intron 30.0%
CTTCATCGAATTTCAATTAG+TGG - chr6.3:36994478-36994497 MS.gene80507:CDS 30.0%
TCGTTCAAAATAGCTCTTAT+GGG - chr6.3:36995267-36995286 MS.gene80507:intron 30.0%
TCTGAATATCACACTATTTC+CGG - chr6.3:36994981-36995000 MS.gene80507:intron 30.0%
! GAAATTGCTGTGTTTGAAAT+AGG + chr6.3:36994298-36994317 None:intergenic 30.0%
! TTTTACTAATGTGGCTAATG+AGG - chr6.3:36995911-36995930 MS.gene80507:CDS 30.0%
!! AATCCATGGATTTGATTTTG+CGG + chr6.3:36995719-36995738 None:intergenic 30.0%
!! TTTACGTGTGCTTTTTATCA+GGG - chr6.3:36995342-36995361 MS.gene80507:intron 30.0%
AAAAAAATTGCTGCAGCATC+TGG - chr6.3:36994907-36994926 MS.gene80507:intron 35.0%
AAAACTTCAGGAAGCAAATC+TGG + chr6.3:36995207-36995226 None:intergenic 35.0%
AAAATCAGATCCTGATCGTT+GGG - chr6.3:36995371-36995390 MS.gene80507:intron 35.0%
AAATAAACTCCCTGAAGATG+AGG - chr6.3:36995842-36995861 MS.gene80507:CDS 35.0%
AAATTAAGACCTACGAAGCA+AGG + chr6.3:36994824-36994843 None:intergenic 35.0%
AGCTTAAGAGTTTGGATTGA+AGG + chr6.3:36995560-36995579 None:intergenic 35.0%
CTGCAGAAAAATCTTTCTGA+AGG - chr6.3:36995870-36995889 MS.gene80507:CDS 35.0%
GACATGAGAATGTTGAAAGA+TGG - chr6.3:36994629-36994648 MS.gene80507:CDS 35.0%
GCTTAAGAGTTTGGATTGAA+GGG + chr6.3:36995559-36995578 None:intergenic 35.0%
TATTAAGCTAGCTGATCCAA+AGG - chr6.3:36995935-36995954 MS.gene80507:CDS 35.0%
TCATACGTCTTCAGAAGAAA+CGG + chr6.3:36994171-36994190 None:intergenic 35.0%
TTTGCAGAACTTCTTTGTGA+AGG - chr6.3:36995659-36995678 MS.gene80507:intron 35.0%
! CTTCAGAAAGATTTTTCTGC+AGG + chr6.3:36995872-36995891 None:intergenic 35.0%
! GCTTCGTAGGTCTTAATTTT+CGG - chr6.3:36994825-36994844 MS.gene80507:intron 35.0%
! TAGCGTGAATTGACTTACAT+AGG + chr6.3:36994346-36994365 None:intergenic 35.0%
! TTTCTGAAGGAGAACTTACT+GGG - chr6.3:36995883-36995902 MS.gene80507:CDS 35.0%
!! ATCCATGGATTTGATTTTGC+GGG + chr6.3:36995718-36995737 None:intergenic 35.0%
!! GTTTACGTGTGCTTTTTATC+AGG - chr6.3:36995341-36995360 MS.gene80507:intron 35.0%
!! TAGGTCTTAATTTTCGGTGT+GGG - chr6.3:36994831-36994850 MS.gene80507:intron 35.0%
!! TTACATAGGTATTGAGCTGA+CGG + chr6.3:36994332-36994351 None:intergenic 35.0%
AACAGCATGGACTTTCAGTA+AGG + chr6.3:36996031-36996050 None:intergenic 40.0%
ATATCTTCGGCAACATTCCA+TGG + chr6.3:36995819-36995838 None:intergenic 40.0%
ATGCTGTTCAGACAATGAAG+AGG - chr6.3:36996043-36996062 MS.gene80507:CDS 40.0%
GAAAATCAGATCCTGATCGT+TGG - chr6.3:36995370-36995389 MS.gene80507:intron 40.0%
TCTTCATTGTCTGAACAGCA+TGG + chr6.3:36996044-36996063 None:intergenic 40.0%
TTCCCGCAAAATCAAATCCA+TGG - chr6.3:36995713-36995732 MS.gene80507:intron 40.0%
TTCTGCTGTAGATCCAGAAA+GGG - chr6.3:36995488-36995507 MS.gene80507:intron 40.0%
! ACGGAGTGAATGATGTGTTT+TGG - chr6.3:36995997-36996016 MS.gene80507:CDS 40.0%
! CGGAGTGAATGATGTGTTTT+GGG - chr6.3:36995998-36996017 MS.gene80507:CDS 40.0%
! CTTTCTGAAGGAGAACTTAC+TGG - chr6.3:36995882-36995901 MS.gene80507:CDS 40.0%
! GTAGGTCTTAATTTTCGGTG+TGG - chr6.3:36994830-36994849 MS.gene80507:intron 40.0%
! TCTTCTGAAGACGTATGACA+TGG - chr6.3:36994173-36994192 MS.gene80507:CDS 40.0%
! TGCTGTGTTTGAAATAGGCT+GGG + chr6.3:36994293-36994312 None:intergenic 40.0%
! TGTGAATTGGTGGGTGATTT+AGG - chr6.3:36994856-36994875 MS.gene80507:intron 40.0%
! TTCAGCTGGAAGATTTTCAG+CGG - chr6.3:36995604-36995623 MS.gene80507:intron 40.0%
! TTCTGAAGGAGAACTTACTG+GGG - chr6.3:36995884-36995903 MS.gene80507:CDS 40.0%
! TTGCTGTGTTTGAAATAGGC+TGG + chr6.3:36994294-36994313 None:intergenic 40.0%
!! CATCTGGATCTTGGTAATGA+TGG - chr6.3:36994923-36994942 MS.gene80507:intron 40.0%
!! TGGGGCATCTTTTACTAATG+TGG - chr6.3:36995902-36995921 MS.gene80507:CDS 40.0%
AAGGTAGCTTGGTTGATCCA+TGG - chr6.3:36995799-36995818 MS.gene80507:intron 45.0%
ACTTCAGGAAGCAAATCTGG+CGG + chr6.3:36995204-36995223 None:intergenic 45.0%
AGAAACGATCTCATCTGTGG+TGG + chr6.3:36994208-36994227 None:intergenic 45.0%
AGAAGAACGCGAGAGAATTC+AGG + chr6.3:36994270-36994289 None:intergenic 45.0%
CCAAGAAACGATCTCATCTG+TGG + chr6.3:36994211-36994230 None:intergenic 45.0%
CCACAGATGAGATCGTTTCT+TGG - chr6.3:36994208-36994227 MS.gene80507:CDS 45.0%
CGTAGACATCTCAAGCATCT+AGG - chr6.3:36995078-36995097 MS.gene80507:intron 45.0%
CTTCTTTGTGAAGGCTCTTC+AGG - chr6.3:36995668-36995687 MS.gene80507:intron 45.0%
GAAACGATCTCATCTGTGGT+GGG + chr6.3:36994207-36994226 None:intergenic 45.0%
GACTGTGGATAGGTTTCTTG+CGG + chr6.3:36995452-36995471 None:intergenic 45.0%
GGATGACTATGACTGTGGAT+AGG + chr6.3:36995462-36995481 None:intergenic 45.0%
GTTCTGCTGTAGATCCAGAA+AGG - chr6.3:36995487-36995506 MS.gene80507:intron 45.0%
TAAGAAGAGGGGATTGTCTC+AGG - chr6.3:36995752-36995771 MS.gene80507:intron 45.0%
TAAGCTAGCTGATCCAAAGG+AGG - chr6.3:36995938-36995957 MS.gene80507:CDS 45.0%
TGAGACAGAAAAGCTTCAGC+TGG - chr6.3:36995590-36995609 MS.gene80507:intron 45.0%
TGGATCAACCAAGCTACCTT+CGG + chr6.3:36995799-36995818 None:intergenic 45.0%
TTCGTTGTTTGTGTCGTGTC+TGG + chr6.3:36994712-36994731 None:intergenic 45.0%
ACGTTACCTGAAGCCTCCTT+TGG + chr6.3:36995954-36995973 None:intergenic 50.0%
AGAACGCGAGAGAATTCAGG+AGG + chr6.3:36994267-36994286 None:intergenic 50.0%
AGATCCAGAAAGGGCAGCAT+TGG - chr6.3:36995497-36995516 MS.gene80507:intron 50.0%
CACAGTCATAGTCATCCTCC+GGG - chr6.3:36995465-36995484 MS.gene80507:intron 50.0%
CATACCTTCTCTGCTTCCGT+CGG + chr6.3:36994145-36994164 None:intergenic 50.0%
CCACAGTCATAGTCATCCTC+CGG - chr6.3:36995464-36995483 MS.gene80507:intron 50.0%
CTACGATGCAGAAGAGAAGC+AGG + chr6.3:36995063-36995082 None:intergenic 50.0%
GAACGCGAGAGAATTCAGGA+GGG + chr6.3:36994266-36994285 None:intergenic 50.0%
GCAAGGACACTCCTCAGATT+AGG + chr6.3:36994807-36994826 None:intergenic 50.0%
GCAGGCTTTCCTCATCTTCA+GGG + chr6.3:36995854-36995873 None:intergenic 50.0%
GTGTGGGTGTGTGAATTGGT+GGG - chr6.3:36994847-36994866 MS.gene80507:intron 50.0%
TCCTCAGATTAGGTGTGTCC+TGG + chr6.3:36994797-36994816 None:intergenic 50.0%
TCTGGATCTACAGCAGAACC+CGG + chr6.3:36995486-36995505 None:intergenic 50.0%
TGCAGGCTTTCCTCATCTTC+AGG + chr6.3:36995855-36995874 None:intergenic 50.0%
TTCTCCAATGCTGCCCTTTC+TGG + chr6.3:36995504-36995523 None:intergenic 50.0%
TTGCTGCAGCATCTGGATCT+TGG - chr6.3:36994914-36994933 MS.gene80507:intron 50.0%
TTGGCAAACTCCCAACGATC+AGG + chr6.3:36995384-36995403 None:intergenic 50.0%
! ATTGAGCTGACGGATGAAGC+TGG + chr6.3:36994322-36994341 None:intergenic 50.0%
! GATTGGAAGGCATGGAGAAG+AGG - chr6.3:36995628-36995647 MS.gene80507:intron 50.0%
! GATTTTCAGCGGCGATTGGA+AGG - chr6.3:36995615-36995634 MS.gene80507:intron 50.0%
! GCAGTGGCTGATGATGATTG+TGG + chr6.3:36994120-36994139 None:intergenic 50.0%
! GGAAGATTTTCAGCGGCGAT+TGG - chr6.3:36995611-36995630 MS.gene80507:intron 50.0%
! TTCGGTGTGGGTGTGTGAAT+TGG - chr6.3:36994843-36994862 MS.gene80507:intron 50.0%
ACCAAGCTACCTTCGGCAAC+AGG + chr6.3:36995792-36995811 None:intergenic 55.0%
AGGAAGCAAATCTGGCGGTG+GGG + chr6.3:36995199-36995218 None:intergenic 55.0%
CAGGAAGCAAATCTGGCGGT+GGG + chr6.3:36995200-36995219 None:intergenic 55.0%
CCGGAGGATGACTATGACTG+TGG + chr6.3:36995467-36995486 None:intergenic 55.0%
CGACGGAAGCAGAGAAGGTA+TGG - chr6.3:36994143-36994162 MS.gene80507:CDS 55.0%
GATTAGGTGTGTCCTGGCGT+CGG + chr6.3:36994791-36994810 None:intergenic 55.0%
GCCAGGACACACCTAATCTG+AGG - chr6.3:36994793-36994812 MS.gene80507:CDS 55.0%
GCTGATCCAAAGGAGGCTTC+AGG - chr6.3:36995945-36995964 MS.gene80507:CDS 55.0%
GGTGTGGGTGTGTGAATTGG+TGG - chr6.3:36994846-36994865 MS.gene80507:intron 55.0%
TCAGGAAGCAAATCTGGCGG+TGG + chr6.3:36995201-36995220 None:intergenic 55.0%
TCCAAGTCGAAGCAGCTGCT+CGG - chr6.3:36995973-36995992 MS.gene80507:CDS 55.0%
!! GAGGAGTGTCCTTGCTTCGT+AGG - chr6.3:36994812-36994831 MS.gene80507:intron 55.0%
CACATGCAGCCTGTTGCCGA+AGG - chr6.3:36995780-36995799 MS.gene80507:intron 60.0%
GCCTGTTGCCGAAGGTAGCT+TGG - chr6.3:36995788-36995807 MS.gene80507:intron 60.0%
GGATCTACAGCAGAACCCGG+AGG + chr6.3:36995483-36995502 None:intergenic 60.0%
TCAGCGGCGATTGGAAGGCA+TGG - chr6.3:36995620-36995639 MS.gene80507:intron 60.0%
TCATCAGCCACTGCTGCCGA+CGG - chr6.3:36994126-36994145 MS.gene80507:CDS 60.0%
GCCGAGCAGCTGCTTCGACT+TGG + chr6.3:36995977-36995996 None:intergenic 65.0%
GCTGCCGACGGAAGCAGAGA+AGG - chr6.3:36994138-36994157 MS.gene80507:CDS 65.0%
GTCGAAGCAGCTGCTCGGCA+CGG - chr6.3:36995978-36995997 MS.gene80507:CDS 65.0%
TCTGCTTCCGTCGGCAGCAG+TGG + chr6.3:36994136-36994155 None:intergenic 65.0%
! CGGTGTTAGACACCGACGCC+AGG - chr6.3:36994776-36994795 MS.gene80507:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 36994105 36996079 36994105 ID=MS.gene80507
chr6.3 mRNA 36994105 36996079 36994105 ID=MS.gene80507.t1;Parent=MS.gene80507
chr6.3 exon 36995837 36996079 36995837 ID=MS.gene80507.t1.exon1;Parent=MS.gene80507.t1
chr6.3 CDS 36995837 36996079 36995837 ID=cds.MS.gene80507.t1;Parent=MS.gene80507.t1
chr6.3 exon 36994105 36994821 36994105 ID=MS.gene80507.t1.exon2;Parent=MS.gene80507.t1
chr6.3 CDS 36994105 36994821 36994105 ID=cds.MS.gene80507.t1;Parent=MS.gene80507.t1
Gene Sequence

>MS.gene80507

ATGAATTCTGCACCACAATCATCATCAGCCACTGCTGCCGACGGAAGCAGAGAAGGTATGGCACCGTTTCTTCTGAAGACGTATGACATGGTAGATGATCCCACCACAGATGAGATCGTTTCTTGGAGCTCTGATAACAAAAGCTTCATCGTTTGGAACCCTCCTGAATTCTCTCGCGTTCTTCTCCCAGCCTATTTCAAACACAGCAATTTCTCCAGCTTCATCCGTCAGCTCAATACCTATGTAAGTCAATTCACGCTATAAGCTTCTTTTGATTATTTCATTGTCACTTTGTATGATCAATTAGTTATTGTCTTTCACTGTGTTTTTGCTTGATTGTTTGTTGTATTTGTATGATTTGGTTTCATTTTGTCTTCATCGAATTTCAATTAGTGGATACTGATTATTATGATGATAATGATCATTAAGAGATTGATGAAAAAAATGATTAGCAAGATAAGAATTAGGTACAACTTTAGATTTGGATTATTTCATTGAGTTTAGTTGGAGATACAAAATGTTGAGACATGAGAATGTTGAAAGATGGATGAGATATTATAGTCTTGTATGGCACTTTTATTCTACGAAACATAAAACATAGACACCAGACACGACACAAACAACGAAACGCAAACAACGACAATGACATAATTCAGTATAATCATATGTGTCGGTGTTAGACACCGACGCCAGGACACACCTAATCTGAGGAGTGTCCTTGCTTCGTAGGTCTTAATTTTCGGTGTGGGTGTGTGAATTGGTGGGTGATTTAGGACTCTAAATGCACTCAAGAAAAACGATCAAAAAAATTGCTGCAGCATCTGGATCTTGGTAATGATGGTACATCGAATTATGAATAAAACCATCAATTTAGTCTCTGAATATCACACTATTTCCGGTTTAGTTCCTGAACTATATAAATATGATAAGTAGTTCCTGATTTTTATTGTTTAATCCTGCTTCTCTTCTGCATCGTAGACATCTCAAGCATCTAGGTTTTCATTTATTGAACTTGTTGAAATTGATATCTAAGTCTTAGATAGGGTTGAAAAATAGAAATCATGCACTTATGGCAATGATTAAAAACTAAGCCCCACCGCCAGATTTGCTTCCTGAAGTTTTTGTTTAATAAAGAATATTCTTGTAGAGATTTAATGGTAAATCGTTCAAAATAGCTCTTATGGGTGTAATGATAAATAATTGTCTAAGAATGTATGTTTTAATTTGTTACGGTTTGTTTACGTGTGCTTTTTATCAGGGATTTCGAAAATCAGATCCTGATCGTTGGGAGTTTGCCAATGAAAAATTTATAAAAGATCAAAAACATCTTCTGACGGATATACACCGCAAGAAACCTATCCACAGTCATAGTCATCCTCCGGGTTCTGCTGTAGATCCAGAAAGGGCAGCATTGGAGAAAGAAATAGAGAAGCTGTGTCGCAAGAAAAATGCCCTTCAATCCAAACTCTTAAGCTATAATTATCTTGAGACAGAAAAGCTTCAGCTGGAAGATTTTCAGCGGCGATTGGAAGGCATGGAGAAGAGGCAGACAAATTTGCAGAACTTCTTTGTGAAGGCTCTTCAGGATTCTTTCATTGTTGAACTTCTTTCCCGCAAAATCAAATCCATGGATTTGGAAACTTATAATAAGAAGAGGGGATTGTCTCAGGTTGATCACATGCAGCCTGTTGCCGAAGGTAGCTTGGTTGATCCATGGAATGTTGCCGAAGATATCTCAAATAAACTCCCTGAAGATGAGGAAAGCCTGCAGAAAAATCTTTCTGAAGGAGAACTTACTGGGGCATCTTTTACTAATGTGGCTAATGAGGCTATTAAGCTAGCTGATCCAAAGGAGGCTTCAGGTAACGTCCAAGTCGAAGCAGCTGCTCGGCACGGAGTGAATGATGTGTTTTGGGAACGATTCCTTACTGAAAGTCCATGCTGTTCAGACAATGAAGAGGAAATGTTCAACTAA
Protein sequence

>MS.gene80507.t1

MNSAPQSSSATAADGSREGMAPFLLKTYDMVDDPTTDEIVSWSSDNKSFIVWNPPEFSRVLLPAYFKHSNFSSFIRQLNTYGFRKSDPDRWEFANEKFIKDQKHLLTDIHRKKPIHSHSHPPGSAVDPERAALEKEIEKLCRKKNALQSKLLSYNYLETEKLQLEDFQRRLEGMEKRQTNLQNFFVKALQDSFIVELLSRKIKSMDLETYNKKRGLSQVDHMQPVAEGSLVDPWNVAEDISNKLPEDEESLQKNLSEGELTGASFTNVANEAIKLADPKEASGNVQVEAAARHGVNDVFWERFLTESPCCSDNEEEMFN