AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80509

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80509.t1 MTR_6g043060 69.452 347 56 4 1 317 1 327 4.02e-166 465
MS.gene80509.t1 MTR_8g083100 75.000 288 39 5 23 277 23 310 3.58e-144 416
MS.gene80509.t1 MTR_8g083100 74.603 63 16 0 257 319 395 457 3.97e-22 96.7
MS.gene80509.t1 MTR_6g044340 65.672 268 64 2 68 319 8 263 1.17e-115 335
MS.gene80509.t1 MTR_2g100680 56.410 312 100 5 1 278 12 321 1.07e-110 329
MS.gene80509.t1 MTR_2g100670 56.884 276 101 5 1 261 14 286 5.88e-95 284
MS.gene80509.t1 MTR_2g100670 56.884 276 101 5 1 261 122 394 3.81e-93 283
MS.gene80509.t1 MTR_6g044430 52.569 253 42 5 100 315 14 225 2.36e-65 205
MS.gene80509.t1 MTR_6g044440 63.128 179 33 2 174 319 1 179 1.47e-64 202
MS.gene80509.t1 MTR_8g087540 36.798 356 165 8 15 315 5 355 4.10e-60 197
MS.gene80509.t1 MTR_5g029680 47.867 211 96 4 22 218 32 242 3.11e-59 197
MS.gene80509.t1 MTR_4g077970 42.105 209 109 3 20 216 10 218 2.13e-50 174
MS.gene80509.t1 MTR_3g104550 33.550 307 188 6 19 317 43 341 3.45e-49 168
MS.gene80509.t1 MTR_6g044350 62.416 149 36 3 174 305 1 146 5.17e-49 160
MS.gene80509.t1 MTR_1g102860 41.256 223 114 6 7 215 20 239 4.77e-48 164
MS.gene80509.t1 MTR_7g095680 43.478 207 107 4 19 215 46 252 1.01e-47 164
MS.gene80509.t1 MTR_1g061170 39.500 200 118 2 20 216 47 246 4.47e-45 159
MS.gene80509.t1 MTR_1g071360 39.535 215 118 3 22 231 32 239 1.27e-44 155
MS.gene80509.t1 MTR_1g071360 39.535 215 118 3 22 231 29 236 2.01e-44 155
MS.gene80509.t1 MTR_8g105780 45.128 195 94 6 20 201 20 214 2.47e-41 147
MS.gene80509.t1 MTR_3g101870 67.742 93 30 0 21 113 23 115 9.54e-41 146
MS.gene80509.t1 MTR_5g017470 59.434 106 43 0 17 122 4 109 3.76e-40 142
MS.gene80509.t1 MTR_6g086805 39.252 214 103 2 20 219 9 209 4.59e-40 144
MS.gene80509.t1 MTR_5g010680 55.556 108 48 0 6 113 8 115 1.17e-38 140
MS.gene80509.t1 MTR_5g089170 64.516 93 33 0 21 113 24 116 2.87e-38 136
MS.gene80509.t1 MTR_2g017890 44.755 143 72 2 12 147 5 147 1.31e-37 138
MS.gene80509.t1 MTR_7g091370 54.545 99 45 0 15 113 15 113 7.03e-36 129
MS.gene80509.t1 MTR_0020s0020 59.140 93 38 0 21 113 32 124 9.18e-36 132
MS.gene80509.t1 MTR_1g100777 56.842 95 41 0 21 115 17 111 9.46e-35 128
MS.gene80509.t1 MTR_6g044510 78.947 76 14 1 246 319 13 88 1.18e-32 116
MS.gene80509.t1 MTR_4g088900 56.701 97 38 1 21 113 32 128 2.37e-32 119
MS.gene80509.t1 MTR_4g045757 57.971 69 28 1 12 79 3 71 4.43e-20 83.2
MS.gene80509.t1 MTR_5g047190 41.237 97 50 2 30 119 1 97 2.43e-19 82.0
MS.gene80509.t1 MTR_6g044500 54.412 68 20 1 205 261 1 68 3.94e-14 67.4
MS.gene80509.t1 MTR_1g061170 29.560 159 101 4 61 216 1 151 1.91e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80509.t1 AT4G13980 53.846 234 106 2 1 232 1 234 3.66e-82 256
MS.gene80509.t1 AT5G16820 46.919 211 97 5 20 216 25 234 1.54e-54 185
MS.gene80509.t1 AT5G16820 46.919 211 97 5 20 216 25 234 1.54e-54 185
MS.gene80509.t1 AT5G45710 36.392 316 172 7 15 315 6 307 2.30e-54 181
MS.gene80509.t1 AT5G45710 36.392 316 172 7 15 315 6 307 2.30e-54 181
MS.gene80509.t1 AT3G02990 42.017 238 122 5 3 228 7 240 1.51e-53 182
MS.gene80509.t1 AT1G32330 43.404 235 114 5 1 216 16 250 2.30e-53 182
MS.gene80509.t1 AT1G32330 43.404 235 114 5 1 216 16 250 2.56e-53 182
MS.gene80509.t1 AT4G17750 44.444 216 99 4 22 216 52 267 4.72e-53 181
MS.gene80509.t1 AT2G26150 40.541 222 124 4 19 232 41 262 1.39e-48 166
MS.gene80509.t1 AT2G26150 40.541 222 124 4 19 232 41 262 1.39e-48 166
MS.gene80509.t1 AT4G18880 45.972 211 104 3 16 220 9 215 2.59e-47 164
MS.gene80509.t1 AT3G22830 37.963 216 121 3 19 221 58 273 2.70e-45 159
MS.gene80509.t1 AT3G51910 40.284 211 109 5 22 226 29 228 3.64e-45 155
MS.gene80509.t1 AT5G03720 40.099 202 113 3 20 213 53 254 2.73e-43 154
MS.gene80509.t1 AT5G03720 40.099 202 113 3 20 213 53 254 2.73e-43 154
MS.gene80509.t1 AT3G24520 42.623 183 84 4 20 202 15 176 7.23e-43 150
MS.gene80509.t1 AT1G67970 43.299 194 93 6 20 201 17 205 1.49e-40 145
MS.gene80509.t1 AT1G46264 51.852 135 46 2 21 136 33 167 1.62e-40 145
MS.gene80509.t1 AT3G63350 38.172 186 100 3 19 196 25 203 1.87e-39 140
MS.gene80509.t1 AT5G54070 40.437 183 103 2 20 201 69 246 3.76e-39 141
MS.gene80509.t1 AT5G43840 39.572 187 101 3 21 205 18 194 1.81e-38 138
MS.gene80509.t1 AT4G36990 54.630 108 47 1 8 115 2 107 4.40e-36 131
MS.gene80509.t1 AT4G11660 60.215 93 37 0 21 113 58 150 5.93e-36 133
MS.gene80509.t1 AT5G62020 47.899 119 61 1 1 118 1 119 3.93e-35 129
MS.gene80509.t1 AT2G41690 49.565 115 58 0 22 136 40 154 2.06e-34 126
MS.gene80509.t1 AT4G18870 42.045 88 51 0 22 109 146 233 1.87e-20 89.7
MS.gene80509.t1 AT4G18870 43.158 95 53 1 15 109 6 99 2.35e-20 89.7
MS.gene80509.t1 AT1G77570 40.217 92 51 3 19 106 22 113 6.76e-15 71.2
MS.gene80509.t1 AT2G26150 29.500 200 126 6 43 232 13 207 1.93e-13 70.1
MS.gene80509.t1 AT2G26150 29.500 200 126 6 43 232 13 207 1.93e-13 70.1
MS.gene80509.t1 AT5G45710 26.667 225 136 7 106 315 1 211 4.57e-13 68.2
MS.gene80509.t1 AT5G45710 26.667 225 136 7 106 315 1 211 4.57e-13 68.2
MS.gene80509.t1 AT4G19630 32.990 97 62 1 19 112 8 104 3.40e-12 63.2

Find 72 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCAATGCTGCCCTTTC+TGG -0.010683 6.3:+36932925 None:intergenic
TGCAGGCTTTCCTCATCTTC+AGG 0.204361 6.3:+36932574 None:intergenic
CTTCTTTGAGAAGGCTCTTC+AGG 0.211830 6.3:-36932758 MS.gene80509:CDS
ATAATTATAGCTTAGGAGTT+TGG 0.257010 6.3:+36932861 None:intergenic
GAAATTGCTGTGTTTGAAAT+AGG 0.280393 6.3:+36934227 None:intergenic
CAAAATCGAATCCATGGATT+TGG 0.330894 6.3:-36932707 MS.gene80509:CDS
CTACAGATGAGATCGTTTCT+TGG 0.332477 6.3:-36934314 MS.gene80509:CDS
CTTTCTGAAGGAGAACTTAC+TGG 0.346936 6.3:-36932544 MS.gene80509:CDS
ACGGAGTGAATGATGTGTTT+TGG 0.356521 6.3:-36932429 MS.gene80509:CDS
CATACCTTCTCTGCTTCCTT+TGG 0.362726 6.3:+36934380 None:intergenic
ATAACAAAAGCTTCATGGTT+TGG 0.362747 6.3:-36934284 MS.gene80509:CDS
CTCTGATAACAAAAGCTTCA+TGG 0.369020 6.3:-36934289 MS.gene80509:CDS
GCAGGCTTTCCTCATCTTCA+GGG 0.373078 6.3:+36932575 None:intergenic
ACGTTACCTGAAGCCTCCTT+TGG 0.398346 6.3:+36932475 None:intergenic
TAAGAAAAGGCGATTGTCTC+AGG 0.414889 6.3:-36932674 MS.gene80509:CDS
ACCAAGCTACCTTCGGCAAC+AGG 0.417304 6.3:+36932637 None:intergenic
AGCTTAGGAGTTTGGATTGA+AGG 0.419735 6.3:+36932869 None:intergenic
TGCTGTGTTTGAAATAGGTT+GGG 0.448167 6.3:+36934232 None:intergenic
TCATACGTCTTCTGAATAAA+CGG 0.454352 6.3:+36934354 None:intergenic
TTGCTGTGTTTGAAATAGGT+TGG 0.457272 6.3:+36934231 None:intergenic
GAGTTTATTTGAGATATCTT+CGG 0.461136 6.3:+36932597 None:intergenic
TGGAAACTTATAATAAGAAA+AGG 0.461564 6.3:-36932687 MS.gene80509:CDS
GCTGATCCAAAGGAGGCTTC+AGG 0.466668 6.3:-36932481 MS.gene80509:CDS
GCCGAGCAGCTGCTTCGACT+TGG 0.471786 6.3:+36932452 None:intergenic
AAAATCAGATCCTGATCGTT+GGG 0.473476 6.3:-36933055 MS.gene80509:CDS
TCTGCTTCCTTTGGCAGCAG+TGG 0.474416 6.3:+36934389 None:intergenic
TCTCAAGATAATTATAGCTT+AGG 0.475528 6.3:+36932854 None:intergenic
CCACAGTCATAGCCATCCTC+CGG 0.477012 6.3:-36932962 MS.gene80509:CDS
TTCCCGCAAAATCGAATCCA+TGG 0.478636 6.3:-36932713 MS.gene80509:CDS
GCTTAGGAGTTTGGATTGAA+GGG 0.480417 6.3:+36932870 None:intergenic
ATTATAAGTTTCCAAATCCA+TGG 0.481800 6.3:+36932696 None:intergenic
CAAAGGAAGCAGAGAAGGTA+TGG 0.493452 6.3:-36934379 MS.gene80509:CDS
AGATCCAGAAAGGGCAGCAT+TGG 0.494908 6.3:-36932929 MS.gene80509:CDS
AGAAACGATCTCATCTGTAG+TGG 0.495784 6.3:+36934317 None:intergenic
TTGACAAACTCCCAACGATC+AGG 0.500192 6.3:+36933045 None:intergenic
TGAGACAGAAAAGCTTCAGC+TGG 0.500611 6.3:-36932836 MS.gene80509:CDS
ATTGAGCTGACGGATGAAGC+TGG 0.506271 6.3:+36934203 None:intergenic
GCCTGTTGCCGAAGGTAGCT+TGG 0.510527 6.3:-36932638 MS.gene80509:CDS
ATATCTTCGGCAACATTCCA+TGG 0.514486 6.3:+36932610 None:intergenic
CCGGAGGATGGCTATGACTG+TGG 0.517314 6.3:+36932962 None:intergenic
TTTCTGAAGGAGAACTTACT+GGG 0.521375 6.3:-36932543 MS.gene80509:CDS
TTTACAGAACTTCTTTGAGA+AGG 0.522492 6.3:-36932767 MS.gene80509:CDS
TGGATCAACCAAGCTACCTT+CGG 0.537647 6.3:+36932630 None:intergenic
AACAGCATGGACTTTCAGTA+AGG 0.541453 6.3:+36932398 None:intergenic
GAAAATCAGATCCTGATCGT+TGG 0.543561 6.3:-36933056 MS.gene80509:CDS
GTCGAAGCAGCTGCTCGGCA+CGG 0.548207 6.3:-36932448 MS.gene80509:CDS
TCAGCGGCGATTGGATGGCA+TGG 0.549084 6.3:-36932806 MS.gene80509:CDS
TCCAAGTCGAAGCAGCTGCT+CGG 0.549998 6.3:-36932453 MS.gene80509:CDS
AAGGTAGCTTGGTTGATCCA+TGG 0.570487 6.3:-36932627 MS.gene80509:CDS
GACTGTGGATAGGTTTCTTG+CGG 0.574041 6.3:+36932977 None:intergenic
CACAGTCATAGCCATCCTCC+GGG 0.578669 6.3:-36932961 MS.gene80509:CDS
AAATAAACTCCCTGAAGATG+AGG 0.580535 6.3:-36932584 MS.gene80509:CDS
GCTGCCAAAGGAAGCAGAGA+AGG 0.583403 6.3:-36934384 MS.gene80509:CDS
GATTGGATGGCATGGAGAAG+AGG 0.590608 6.3:-36932798 MS.gene80509:CDS
AGATCGAAAACATGTTCTGA+CGG 0.592356 6.3:-36933007 MS.gene80509:CDS
TCATCAGCCACTGCTGCCAA+AGG 0.593850 6.3:-36934396 MS.gene80509:CDS
CACATGCAGCCTGTTGCCGA+AGG 0.596050 6.3:-36932646 MS.gene80509:CDS
TCTTCATTGTCTGAACAGCA+TGG 0.597736 6.3:+36932385 None:intergenic
TTACATAGGTATTGAGCTGA+CGG 0.598207 6.3:+36934193 None:intergenic
CTACAGCAAAACCCGGAGGA+TGG 0.600277 6.3:+36932950 None:intergenic
GGATGGCTATGACTGTGGAT+AGG 0.610377 6.3:+36932967 None:intergenic
GAAACGATCTCATCTGTAGT+GGG 0.620712 6.3:+36934318 None:intergenic
AGAACGCGAGAGAATTCATG+AGG 0.636031 6.3:+36934258 None:intergenic
ATGCTGTTCAGACAATGAAG+AGG 0.637659 6.3:-36932383 MS.gene80509:CDS
TATTAAGCTAGCTGATCCAA+AGG 0.648739 6.3:-36932491 MS.gene80509:CDS
TATTCAGAAGACGTATGACA+TGG 0.665030 6.3:-36934349 MS.gene80509:CDS
TCTGGATCTACAGCAAAACC+CGG 0.667096 6.3:+36932943 None:intergenic
TAAGCTAGCTGATCCAAAGG+AGG 0.675297 6.3:-36932488 MS.gene80509:CDS
TTCTGAAGGAGAACTTACTG+GGG 0.695684 6.3:-36932542 MS.gene80509:CDS
GAACGCGAGAGAATTCATGA+GGG 0.704486 6.3:+36934259 None:intergenic
GCAGTGGCTGATGATGATTG+TGG 0.706595 6.3:+36934405 None:intergenic
GGATCTACAGCAAAACCCGG+AGG 0.768670 6.3:+36932946 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATCATATTTATATAGTTC+TGG + chr6.3:36933374-36933393 None:intergenic 15.0%
!!! ATGTATGTTTTAATTTGTTA+CGG - chr6.3:36933675-36933694 MS.gene80509:intron 15.0%
!! AAATATAAATCATGCACTTA+TGG - chr6.3:36933509-36933528 MS.gene80509:intron 20.0%
!! ACTAATTGATCATACAAAAT+AGG + chr6.3:36932657-36932676 None:intergenic 20.0%
!! ATATTCAAAGACTAAATTGA+TGG + chr6.3:36933330-36933349 None:intergenic 20.0%
!! ATATTCTTGTAGAGATTTAA+TGG - chr6.3:36933594-36933613 MS.gene80509:intron 20.0%
!! ATTATTTCATTGAGTTTAGT+TGG - chr6.3:36932854-36932873 MS.gene80509:CDS 20.0%
!! TGATTAGTAAGATAAGAATT+AGG - chr6.3:36932814-36932833 MS.gene80509:CDS 20.0%
!! TGGAAACTTATAATAAGAAA+AGG - chr6.3:36934099-36934118 MS.gene80509:intron 20.0%
!!! ATACTGAATTATGTCATTTT+CGG + chr6.3:36933008-36933027 None:intergenic 20.0%
!!! TAAGATATTATAGTCTTGTA+TGG - chr6.3:36932917-36932936 MS.gene80509:CDS 20.0%
!!! TTTGTTGTATTTGTATGATT+TGG - chr6.3:36932708-36932727 MS.gene80509:CDS 20.0%
! AAAAAAATTTCTGTAGCATC+TGG - chr6.3:36933267-36933286 MS.gene80509:intron 25.0%
! AAATCGTTCAAAAAGTTTGT+AGG - chr6.3:36933618-36933637 MS.gene80509:intron 25.0%
! ATAATTATAGCTTAGGAGTT+TGG + chr6.3:36933928-36933947 None:intergenic 25.0%
! ATTAGGTACAACTTTAGATT+TGG - chr6.3:36932831-36932850 MS.gene80509:CDS 25.0%
! ATTATAAGTTTCCAAATCCA+TGG + chr6.3:36934093-36934112 None:intergenic 25.0%
! CTTCATTACACAAAAACTTT+AGG + chr6.3:36933573-36933592 None:intergenic 25.0%
! TCTCAAGATAATTATAGCTT+AGG + chr6.3:36933935-36933954 None:intergenic 25.0%
! TGATATCTAAGTCTTAGATA+GGG - chr6.3:36933481-36933500 MS.gene80509:intron 25.0%
! TTCAGTATAATCATATGTGT+CGG - chr6.3:36933020-36933039 MS.gene80509:CDS 25.0%
! TTGATATCTAAGTCTTAGAT+AGG - chr6.3:36933480-36933499 MS.gene80509:intron 25.0%
! TTTGAATATCACACTATTTC+CGG - chr6.3:36933341-36933360 MS.gene80509:intron 25.0%
!! GAGTTTATTTGAGATATCTT+CGG + chr6.3:36934192-36934211 None:intergenic 25.0%
!!! ATTTACATGTGCTTTTTATC+AGG - chr6.3:36933701-36933720 MS.gene80509:intron 25.0%
!!! TTTACATGTGCTTTTTATCA+GGG - chr6.3:36933702-36933721 MS.gene80509:intron 25.0%
ATAACAAAAGCTTCATGGTT+TGG - chr6.3:36932502-36932521 MS.gene80509:CDS 30.0%
CAAAAAGTTTGTAGGTCTTA+TGG - chr6.3:36933626-36933645 MS.gene80509:intron 30.0%
CTTCGTCGAATTTAAATTAG+TGG - chr6.3:36932742-36932761 MS.gene80509:CDS 30.0%
TCATACGTCTTCTGAATAAA+CGG + chr6.3:36932435-36932454 None:intergenic 30.0%
! AAAACTTTAGGAAGCAAATC+TGG + chr6.3:36933561-36933580 None:intergenic 30.0%
! ATAATCATATGTGTCGGTAT+CGG - chr6.3:36933026-36933045 MS.gene80509:CDS 30.0%
! GAAATTGCTGTGTTTGAAAT+AGG + chr6.3:36932562-36932581 None:intergenic 30.0%
! TAGCATGAATTGACTTACAT+AGG + chr6.3:36932610-36932629 None:intergenic 30.0%
! TTTACAGAACTTCTTTGAGA+AGG - chr6.3:36934019-36934038 MS.gene80509:intron 30.0%
!! ATATAGTTCTGGAACTAAAC+CGG + chr6.3:36933363-36933382 None:intergenic 30.0%
AAAATCAGATCCTGATCGTT+GGG - chr6.3:36933731-36933750 MS.gene80509:intron 35.0%
AAATAAACTCCCTGAAGATG+AGG - chr6.3:36934202-36934221 MS.gene80509:CDS 35.0%
AAATTAAGACCTACGAAGCA+AGG + chr6.3:36933088-36933107 None:intergenic 35.0%
AATTCGTAGGTCTTAATCTG+AGG - chr6.3:36933196-36933215 MS.gene80509:intron 35.0%
AGAAGCAGGATTAAACAATC+AGG + chr6.3:36933403-36933422 None:intergenic 35.0%
AGATCGAAAACATGTTCTGA+CGG - chr6.3:36933779-36933798 MS.gene80509:intron 35.0%
ATACAAAATGTTGAGACCTG+AGG - chr6.3:36932879-36932898 MS.gene80509:CDS 35.0%
CAAAATCGAATCCATGGATT+TGG - chr6.3:36934079-36934098 MS.gene80509:intron 35.0%
CTCTGATAACAAAAGCTTCA+TGG - chr6.3:36932497-36932516 MS.gene80509:CDS 35.0%
CTGCAGAAAAATCTTTCTGA+AGG - chr6.3:36934230-36934249 MS.gene80509:CDS 35.0%
TATTAAGCTAGCTGATCCAA+AGG - chr6.3:36934295-36934314 MS.gene80509:CDS 35.0%
TATTCAGAAGACGTATGACA+TGG - chr6.3:36932437-36932456 MS.gene80509:CDS 35.0%
! AATCCATGGATTCGATTTTG+CGG + chr6.3:36934079-36934098 None:intergenic 35.0%
! AATTGGTGGGTGATTTAGAT+AGG - chr6.3:36933124-36933143 MS.gene80509:intron 35.0%
! ATTGGTGGGTGATTTAGATA+GGG - chr6.3:36933125-36933144 MS.gene80509:intron 35.0%
! CTTCAGAAAGATTTTTCTGC+AGG + chr6.3:36934232-36934251 None:intergenic 35.0%
! GCTTCGTAGGTCTTAATTTT+CGG - chr6.3:36933089-36933108 MS.gene80509:intron 35.0%
! TGCTGTGTTTGAAATAGGTT+GGG + chr6.3:36932557-36932576 None:intergenic 35.0%
! TTGCTGTGTTTGAAATAGGT+TGG + chr6.3:36932558-36932577 None:intergenic 35.0%
! TTTCTGAAGGAGAACTTACT+GGG - chr6.3:36934243-36934262 MS.gene80509:CDS 35.0%
! TTTTACTAACGTGGCTAATG+AGG - chr6.3:36934271-36934290 MS.gene80509:CDS 35.0%
!! ATCTTGGTAATGATGGTACA+TGG - chr6.3:36933290-36933309 MS.gene80509:intron 35.0%
!! TAGGTCTTAATTTTCGGTGT+GGG - chr6.3:36933095-36933114 MS.gene80509:intron 35.0%
!! TTACATAGGTATTGAGCTGA+CGG + chr6.3:36932596-36932615 None:intergenic 35.0%
!! TTTTGCTGTAGATCCAGAAA+GGG - chr6.3:36933848-36933867 MS.gene80509:intron 35.0%
AACAGCATGGACTTTCAGTA+AGG + chr6.3:36934391-36934410 None:intergenic 40.0%
AGAAACGATCTCATCTGTAG+TGG + chr6.3:36932472-36932491 None:intergenic 40.0%
AGCTTAGGAGTTTGGATTGA+AGG + chr6.3:36933920-36933939 None:intergenic 40.0%
ATATCTTCGGCAACATTCCA+TGG + chr6.3:36934179-36934198 None:intergenic 40.0%
ATGCTGTTCAGACAATGAAG+AGG - chr6.3:36934403-36934422 MS.gene80509:CDS 40.0%
CTACAGATGAGATCGTTTCT+TGG - chr6.3:36932472-36932491 MS.gene80509:CDS 40.0%
GAAAATCAGATCCTGATCGT+TGG - chr6.3:36933730-36933749 MS.gene80509:intron 40.0%
GAAACGATCTCATCTGTAGT+GGG + chr6.3:36932471-36932490 None:intergenic 40.0%
GCTTAGGAGTTTGGATTGAA+GGG + chr6.3:36933919-36933938 None:intergenic 40.0%
TAAGAAAAGGCGATTGTCTC+AGG - chr6.3:36934112-36934131 MS.gene80509:intron 40.0%
TCTTCATTGTCTGAACAGCA+TGG + chr6.3:36934404-36934423 None:intergenic 40.0%
TTTCTGTAGCATCTGGATCT+TGG - chr6.3:36933274-36933293 MS.gene80509:intron 40.0%
! ACGGAGTGAATGATGTGTTT+TGG - chr6.3:36934357-36934376 MS.gene80509:CDS 40.0%
! ACTTTAGGAAGCAAATCTGG+CGG + chr6.3:36933558-36933577 None:intergenic 40.0%
! ATCCATGGATTCGATTTTGC+GGG + chr6.3:36934078-36934097 None:intergenic 40.0%
! CGGAGTGAATGATGTGTTTT+GGG - chr6.3:36934358-36934377 MS.gene80509:CDS 40.0%
! CTTTCTGAAGGAGAACTTAC+TGG - chr6.3:36934242-36934261 MS.gene80509:CDS 40.0%
! GTAGGTCTTAATTTTCGGTG+TGG - chr6.3:36933094-36933113 MS.gene80509:intron 40.0%
! TTCAGCTGGAAGATTTTCAG+CGG - chr6.3:36933964-36933983 MS.gene80509:intron 40.0%
! TTCTGAAGGAGAACTTACTG+GGG - chr6.3:36934244-36934263 MS.gene80509:CDS 40.0%
!! CATCTGGATCTTGGTAATGA+TGG - chr6.3:36933283-36933302 MS.gene80509:intron 40.0%
!! GTTTTGCTGTAGATCCAGAA+AGG - chr6.3:36933847-36933866 MS.gene80509:intron 40.0%
!! TCTTAATTTTCGGTGTGGGT+GGG - chr6.3:36933099-36933118 MS.gene80509:intron 40.0%
AAGGTAGCTTGGTTGATCCA+TGG - chr6.3:36934159-36934178 MS.gene80509:intron 45.0%
AATCCGAGGAGTGAATTGGT+GGG - chr6.3:36933153-36933172 MS.gene80509:intron 45.0%
AGAACGCGAGAGAATTCATG+AGG + chr6.3:36932531-36932550 None:intergenic 45.0%
ATCCATCTTTCAGCATCCTC+AGG + chr6.3:36932898-36932917 None:intergenic 45.0%
CAAAGGAAGCAGAGAAGGTA+TGG - chr6.3:36932407-36932426 MS.gene80509:CDS 45.0%
CATACCTTCTCTGCTTCCTT+TGG + chr6.3:36932409-36932428 None:intergenic 45.0%
CCAATTCACTCCTCGGATTA+AGG + chr6.3:36933152-36933171 None:intergenic 45.0%
CCTTAATCCGAGGAGTGAAT+TGG - chr6.3:36933149-36933168 MS.gene80509:intron 45.0%
CGTACACATCTCAAGCATCT+AGG - chr6.3:36933432-36933451 MS.gene80509:intron 45.0%
CTTGAGTGCATTTAGTGTCC+TGG + chr6.3:36933237-36933256 None:intergenic 45.0%
GAACGCGAGAGAATTCATGA+GGG + chr6.3:36932530-36932549 None:intergenic 45.0%
GACTGTGGATAGGTTTCTTG+CGG + chr6.3:36933812-36933831 None:intergenic 45.0%
TAAGCTAGCTGATCCAAAGG+AGG - chr6.3:36934298-36934317 MS.gene80509:CDS 45.0%
TAATCCGAGGAGTGAATTGG+TGG - chr6.3:36933152-36933171 MS.gene80509:intron 45.0%
TAGATAGGGTCCTTAATCCG+AGG - chr6.3:36933139-36933158 MS.gene80509:intron 45.0%
TCTGGATCTACAGCAAAACC+CGG + chr6.3:36933846-36933865 None:intergenic 45.0%
TGAGACAGAAAAGCTTCAGC+TGG - chr6.3:36933950-36933969 MS.gene80509:intron 45.0%
TGGATCAACCAAGCTACCTT+CGG + chr6.3:36934159-36934178 None:intergenic 45.0%
TTCCCGCAAAATCGAATCCA+TGG - chr6.3:36934073-36934092 MS.gene80509:intron 45.0%
TTCGTTGTTTGTGTCGTGTC+TGG + chr6.3:36932976-36932995 None:intergenic 45.0%
TTGACAAACTCCCAACGATC+AGG + chr6.3:36933744-36933763 None:intergenic 45.0%
! AATTGGTGGGTGAATTGGTG+TGG - chr6.3:36933166-36933185 MS.gene80509:intron 45.0%
! ATTGGTGGGTGAATTGGTGT+GGG - chr6.3:36933167-36933186 MS.gene80509:intron 45.0%
! CTTCTTTGAGAAGGCTCTTC+AGG - chr6.3:36934028-36934047 MS.gene80509:intron 45.0%
! GAGTGAATTGGTGGGTGAAT+TGG - chr6.3:36933161-36933180 MS.gene80509:intron 45.0%
!! GTCTTAATTTTCGGTGTGGG+TGG - chr6.3:36933098-36933117 MS.gene80509:intron 45.0%
!! TGGGGCATCTTTTACTAACG+TGG - chr6.3:36934262-36934281 MS.gene80509:CDS 45.0%
ACGTTACCTGAAGCCTCCTT+TGG + chr6.3:36934314-36934333 None:intergenic 50.0%
AGATCCAGAAAGGGCAGCAT+TGG - chr6.3:36933857-36933876 MS.gene80509:intron 50.0%
GACCTGAGGATGCTGAAAGA+TGG - chr6.3:36932893-36932912 MS.gene80509:CDS 50.0%
GATTGGATGGCATGGAGAAG+AGG - chr6.3:36933988-36934007 MS.gene80509:intron 50.0%
GCAGGCTTTCCTCATCTTCA+GGG + chr6.3:36934214-36934233 None:intergenic 50.0%
GGATGGCTATGACTGTGGAT+AGG + chr6.3:36933822-36933841 None:intergenic 50.0%
GTACGATGCAGAAGAGAAGC+AGG + chr6.3:36933417-36933436 None:intergenic 50.0%
TACGAAGCAAGGACACTCCT+CGG + chr6.3:36933077-36933096 None:intergenic 50.0%
TAGGAAGCAAATCTGGCGGT+GGG + chr6.3:36933554-36933573 None:intergenic 50.0%
TCACCCACCAATTCACTCCT+CGG + chr6.3:36933159-36933178 None:intergenic 50.0%
TGCAGGCTTTCCTCATCTTC+AGG + chr6.3:36934215-36934234 None:intergenic 50.0%
TTAGGAAGCAAATCTGGCGG+TGG + chr6.3:36933555-36933574 None:intergenic 50.0%
TTCTCCAATGCTGCCCTTTC+TGG + chr6.3:36933864-36933883 None:intergenic 50.0%
TTTAGTGTCCTGGCGAAGCA+AGG + chr6.3:36933227-36933246 None:intergenic 50.0%
! ATTGAGCTGACGGATGAAGC+TGG + chr6.3:36932586-36932605 None:intergenic 50.0%
! GATTTTCAGCGGCGATTGGA+TGG - chr6.3:36933975-36933994 MS.gene80509:intron 50.0%
! GCAGTGGCTGATGATGATTG+TGG + chr6.3:36932384-36932403 None:intergenic 50.0%
! GGAAGATTTTCAGCGGCGAT+TGG - chr6.3:36933971-36933990 MS.gene80509:intron 50.0%
ACCAAGCTACCTTCGGCAAC+AGG + chr6.3:36934152-36934171 None:intergenic 55.0%
AGGAAGCAAATCTGGCGGTG+GGG + chr6.3:36933553-36933572 None:intergenic 55.0%
CACAGTCATAGCCATCCTCC+GGG - chr6.3:36933825-36933844 MS.gene80509:intron 55.0%
CCACAGTCATAGCCATCCTC+CGG - chr6.3:36933824-36933843 MS.gene80509:intron 55.0%
CTACAGCAAAACCCGGAGGA+TGG + chr6.3:36933839-36933858 None:intergenic 55.0%
GCAAGGACACTCCTCGGATT+AGG + chr6.3:36933071-36933090 None:intergenic 55.0%
GCTGATCCAAAGGAGGCTTC+AGG - chr6.3:36934305-36934324 MS.gene80509:CDS 55.0%
GCTGCCAAAGGAAGCAGAGA+AGG - chr6.3:36932402-36932421 MS.gene80509:CDS 55.0%
GGATCTACAGCAAAACCCGG+AGG + chr6.3:36933843-36933862 None:intergenic 55.0%
GTGGGTGAATTGGTGTGGGT+GGG - chr6.3:36933171-36933190 MS.gene80509:intron 55.0%
GTGTGGGTGGGTGAATTCGT+AGG - chr6.3:36933183-36933202 MS.gene80509:intron 55.0%
GTGTGGGTGGGTGAATTGGT+GGG - chr6.3:36933111-36933130 MS.gene80509:intron 55.0%
TCATCAGCCACTGCTGCCAA+AGG - chr6.3:36932390-36932409 MS.gene80509:CDS 55.0%
TCCAAGTCGAAGCAGCTGCT+CGG - chr6.3:36934333-36934352 MS.gene80509:CDS 55.0%
TCTGCTTCCTTTGGCAGCAG+TGG + chr6.3:36932400-36932419 None:intergenic 55.0%
! TCCTCGGATTAGGTGTGTCC+TGG + chr6.3:36933061-36933080 None:intergenic 55.0%
! TTCGGTGTGGGTGGGTGAAT+TGG - chr6.3:36933107-36933126 MS.gene80509:intron 55.0%
!! GAGGAGTGTCCTTGCTTCGT+AGG - chr6.3:36933076-36933095 MS.gene80509:intron 55.0%
CACATGCAGCCTGTTGCCGA+AGG - chr6.3:36934140-36934159 MS.gene80509:intron 60.0%
CCGGAGGATGGCTATGACTG+TGG + chr6.3:36933827-36933846 None:intergenic 60.0%
GCCAGGACACACCTAATCCG+AGG - chr6.3:36933057-36933076 MS.gene80509:CDS 60.0%
GCCTGTTGCCGAAGGTAGCT+TGG - chr6.3:36934148-36934167 MS.gene80509:intron 60.0%
GGTGGGTGAATTGGTGTGGG+TGG - chr6.3:36933170-36933189 MS.gene80509:intron 60.0%
GGTGTGGGTGGGTGAATTGG+TGG - chr6.3:36933110-36933129 MS.gene80509:intron 60.0%
TCAGCGGCGATTGGATGGCA+TGG - chr6.3:36933980-36933999 MS.gene80509:intron 60.0%
!! AGGAGTGTCCTTGCTTCGCC+AGG - chr6.3:36933216-36933235 MS.gene80509:intron 60.0%
GCCGAGCAGCTGCTTCGACT+TGG + chr6.3:36934337-36934356 None:intergenic 65.0%
GTCGAAGCAGCTGCTCGGCA+CGG - chr6.3:36934338-36934357 MS.gene80509:CDS 65.0%
! CGGTATCGGACACTGACGCC+AGG - chr6.3:36933040-36933059 MS.gene80509:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 36932369 36934439 36932369 ID=MS.gene80509
chr6.3 mRNA 36932369 36934439 36932369 ID=MS.gene80509.t1;Parent=MS.gene80509
chr6.3 exon 36934197 36934439 36934197 ID=MS.gene80509.t1.exon1;Parent=MS.gene80509.t1
chr6.3 CDS 36934197 36934439 36934197 ID=cds.MS.gene80509.t1;Parent=MS.gene80509.t1
chr6.3 exon 36932369 36933085 36932369 ID=MS.gene80509.t1.exon2;Parent=MS.gene80509.t1
chr6.3 CDS 36932369 36933085 36932369 ID=cds.MS.gene80509.t1;Parent=MS.gene80509.t1
Gene Sequence

>MS.gene80509

ATGAATTCTGCACCACAATCATCATCAGCCACTGCTGCCAAAGGAAGCAGAGAAGGTATGGCACCGTTTATTCAGAAGACGTATGACATGGTAGATGATCCCACTACAGATGAGATCGTTTCTTGGAGCTCTGATAACAAAAGCTTCATGGTTTGGAACCCTCATGAATTCTCTCGCGTTCTTCTCCCAACCTATTTCAAACACAGCAATTTCTCCAGCTTCATCCGTCAGCTCAATACCTATGTAAGTCAATTCATGCTATAAGCTTCTTTTGATTATTTCATTCCTATTTTGTATGATCAATTAGTTATTGTCTTTCACTGTGTTTTTGCTTGATTGTTTGTTGTATTTGTATGATTTGGTTTCATTGTGTCTTCGTCGAATTTAAATTAGTGGATACTGATTATAATGATGATCATGATCATTAAGAGATTGATGAAAAAAATGATTAGTAAGATAAGAATTAGGTACAACTTTAGATTTGGATTATTTCATTGAGTTTAGTTGGAGATACAAAATGTTGAGACCTGAGGATGCTGAAAGATGGATAAGATATTATAGTCTTGTATGGCACTTTTATTCTATGAAACATAAAACACAGACACCAGACACGACACAAACAACGAAACGCAAACACCGAAAATGACATAATTCAGTATAATCATATGTGTCGGTATCGGACACTGACGCCAGGACACACCTAATCCGAGGAGTGTCCTTGCTTCGTAGGTCTTAATTTTCGGTGTGGGTGGGTGAATTGGTGGGTGATTTAGATAGGGTCCTTAATCCGAGGAGTGAATTGGTGGGTGAATTGGTGTGGGTGGGTGAATTCGTAGGTCTTAATCTGAGGAGTGTCCTTGCTTCGCCAGGACACTAAATGCACTCAAGAAAAACGATCAAAAAAATTTCTGTAGCATCTGGATCTTGGTAATGATGGTACATGGAATCATGAATAAAACCATCAATTTAGTCTTTGAATATCACACTATTTCCGGTTTAGTTCCAGAACTATATAAATATGATAAGTAGTTCCTGATTGTTTAATCCTGCTTCTCTTCTGCATCGTACACATCTCAAGCATCTAGGTTTTCAATTATTGAACTTGTTGAAATTGATATCTAAGTCTTAGATAGGGTTGAAAAATATAAATCATGCACTTATGGCAATGATTAAAAACTAATCCCCACCGCCAGATTTGCTTCCTAAAGTTTTTGTGTAATGAAGAATATTCTTGTAGAGATTTAATGGTAAATCGTTCAAAAAGTTTGTAGGTCTTATGGTTGTAATGATAAATAATTGTTGAAGAATGTATGTTTTAATTTGTTACGGTTTATTTACATGTGCTTTTTATCAGGGATTTCGAAAATCAGATCCTGATCGTTGGGAGTTTGTCAATGAAAAATTTATAAAAGATCGAAAACATGTTCTGACGGATATACACCGCAAGAAACCTATCCACAGTCATAGCCATCCTCCGGGTTTTGCTGTAGATCCAGAAAGGGCAGCATTGGAGAAAGAAATAGAGAAGCTGTTTCACGAGAAAAATGCCCTTCAATCCAAACTCCTAAGCTATAATTATCTTGAGACAGAAAAGCTTCAGCTGGAAGATTTTCAGCGGCGATTGGATGGCATGGAGAAGAGGCAGACAAATTTACAGAACTTCTTTGAGAAGGCTCTTCAGGATTCTTTTATTGTTGAACTTCTTTCCCGCAAAATCGAATCCATGGATTTGGAAACTTATAATAAGAAAAGGCGATTGTCTCAGGTTGATCACATGCAGCCTGTTGCCGAAGGTAGCTTGGTTGATCCATGGAATGTTGCCGAAGATATCTCAAATAAACTCCCTGAAGATGAGGAAAGCCTGCAGAAAAATCTTTCTGAAGGAGAACTTACTGGGGCATCTTTTACTAACGTGGCTAATGAGGCTATTAAGCTAGCTGATCCAAAGGAGGCTTCAGGTAACGTCCAAGTCGAAGCAGCTGCTCGGCACGGAGTGAATGATGTGTTTTGGGAACAATTCCTTACTGAAAGTCCATGCTGTTCAGACAATGAAGAGGAAATGTTCAACTAA
Protein sequence

>MS.gene80509.t1

MNSAPQSSSATAAKGSREGMAPFIQKTYDMVDDPTTDEIVSWSSDNKSFMVWNPHEFSRVLLPTYFKHSNFSSFIRQLNTYGFRKSDPDRWEFVNEKFIKDRKHVLTDIHRKKPIHSHSHPPGFAVDPERAALEKEIEKLFHEKNALQSKLLSYNYLETEKLQLEDFQRRLDGMEKRQTNLQNFFEKALQDSFIVELLSRKIESMDLETYNKKRRLSQVDHMQPVAEGSLVDPWNVAEDISNKLPEDEESLQKNLSEGELTGASFTNVANEAIKLADPKEASGNVQVEAAARHGVNDVFWEQFLTESPCCSDNEEEMFN