AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81101

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81101.t1 MTR_2g061030 91.239 331 12 4 1 317 1 328 0.0 536
MS.gene81101.t1 MTR_1g054285 51.818 330 94 11 1 315 1 280 3.69e-83 253
MS.gene81101.t1 MTR_7g093430 50.779 321 91 10 1 302 1 273 2.50e-82 251
MS.gene81101.t1 MTR_4g100550 55.777 251 72 6 75 313 22 245 1.48e-72 226
MS.gene81101.t1 MTR_5g013010 61.502 213 47 8 111 312 72 260 1.59e-69 219
MS.gene81101.t1 MTR_4g097600 76.119 134 29 1 168 301 109 239 4.44e-67 211
MS.gene81101.t1 MTR_5g014890 63.462 156 50 2 175 327 113 264 3.26e-61 196
MS.gene81101.t1 MTR_8g006705 57.277 213 65 4 102 300 58 258 5.53e-61 197
MS.gene81101.t1 MTR_1g017090 63.433 134 42 2 171 302 117 245 4.68e-53 176
MS.gene81101.t1 MTR_4g084750 66.129 124 35 2 184 300 59 182 4.36e-49 163
MS.gene81101.t1 MTR_3g103590 66.667 129 39 2 175 303 134 258 1.65e-44 154
MS.gene81101.t1 MTR_2g038625 54.545 132 57 2 189 318 82 212 5.93e-38 134
MS.gene81101.t1 MTR_2g038580 54.545 132 57 2 189 318 82 212 5.93e-38 134
MS.gene81101.t1 MTR_4g126900 51.799 139 52 3 191 326 90 216 9.25e-35 126
MS.gene81101.t1 MTR_5g013010 55.000 140 29 7 111 240 72 187 2.83e-29 112
MS.gene81101.t1 MTR_6g007647 42.105 114 66 0 189 302 33 146 1.65e-18 84.0
MS.gene81101.t1 MTR_7g010020 45.946 111 55 3 175 281 67 176 1.81e-18 84.0
MS.gene81101.t1 MTR_6g007647 42.105 114 66 0 189 302 67 180 2.11e-18 84.3
MS.gene81101.t1 MTR_1g061660 44.086 93 52 0 185 277 55 147 2.87e-18 82.8
MS.gene81101.t1 MTR_4g107650 44.355 124 63 2 159 276 59 182 2.92e-18 84.7
MS.gene81101.t1 MTR_6g011610 41.085 129 51 2 154 275 79 189 3.13e-18 83.6
MS.gene81101.t1 MTR_6g011610 41.860 129 50 3 154 275 60 170 5.07e-18 82.8
MS.gene81101.t1 MTR_5g038280 35.196 179 102 5 106 275 7 180 1.10e-17 82.8
MS.gene81101.t1 MTR_7g103340 35.862 145 91 2 183 325 57 201 1.15e-17 81.3
MS.gene81101.t1 MTR_3g080100 43.119 109 55 1 175 276 6 114 1.96e-17 80.1
MS.gene81101.t1 MTR_5g039000 40.152 132 74 2 145 276 38 164 2.09e-17 81.3
MS.gene81101.t1 MTR_8g468210 38.411 151 73 4 139 282 37 174 2.54e-17 81.3
MS.gene81101.t1 MTR_3g080100 39.200 125 67 2 161 276 29 153 2.70e-17 80.5
MS.gene81101.t1 MTR_3g086790 36.986 146 80 4 176 310 46 190 1.21e-16 79.3
MS.gene81101.t1 MTR_8g089895 46.875 96 44 1 188 276 53 148 4.25e-16 78.2
MS.gene81101.t1 MTR_8g469430 44.681 94 45 1 189 275 89 182 2.94e-15 75.1
MS.gene81101.t1 MTR_5g019650 45.882 85 46 0 189 273 53 137 6.27e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81101.t1 AT5G06710 50.000 326 132 8 1 310 1 311 1.51e-85 261
MS.gene81101.t1 AT4G37790 61.236 178 58 3 151 320 83 257 5.31e-65 206
MS.gene81101.t1 AT2G44910 58.209 201 55 3 113 304 99 279 7.94e-65 207
MS.gene81101.t1 AT3G60390 59.896 192 66 3 113 300 90 274 8.67e-64 204
MS.gene81101.t1 AT5G47370 51.128 266 91 10 72 319 17 261 1.44e-63 203
MS.gene81101.t1 AT4G16780 64.198 162 49 3 163 320 108 264 6.62e-61 196
MS.gene81101.t1 AT2G22800 75.862 116 28 0 186 301 110 225 1.38e-59 192
MS.gene81101.t1 AT4G17460 50.885 226 93 5 90 307 39 254 7.28e-59 191
MS.gene81101.t1 AT2G01430 57.042 142 59 2 175 316 125 264 1.42e-47 161
MS.gene81101.t1 AT1G70920 55.479 146 49 3 173 318 26 155 2.88e-41 142
MS.gene81101.t1 AT1G70920 55.479 146 49 3 173 318 56 185 3.41e-41 143
MS.gene81101.t1 AT5G06710 41.406 256 119 8 1 240 1 241 6.39e-39 139
MS.gene81101.t1 AT2G01430 63.636 66 24 0 175 240 125 190 7.18e-21 89.7
MS.gene81101.t1 AT5G03790 37.008 127 79 1 184 309 72 198 4.82e-18 82.4
MS.gene81101.t1 AT5G06710 37.118 229 113 11 1 213 1 214 6.73e-18 82.0
MS.gene81101.t1 AT5G06710 37.118 229 113 11 1 213 1 214 6.73e-18 82.0
MS.gene81101.t1 AT1G69780 44.554 101 56 0 176 276 72 172 2.63e-17 81.3
MS.gene81101.t1 AT1G26960 36.242 149 72 3 177 321 63 192 1.03e-16 79.0
MS.gene81101.t1 AT1G27045 51.852 81 38 1 175 254 10 90 3.52e-16 75.9
MS.gene81101.t1 AT1G27045 51.852 81 38 1 175 254 10 90 3.52e-16 75.9
MS.gene81101.t1 AT1G27045 51.852 81 38 1 175 254 2 82 3.94e-16 75.5
MS.gene81101.t1 AT1G27045 51.852 81 38 1 175 254 53 133 5.74e-16 76.3
MS.gene81101.t1 AT5G15150 40.909 110 54 2 173 275 103 208 7.70e-16 77.4
MS.gene81101.t1 AT2G36610 40.196 102 53 1 184 277 66 167 9.36e-16 74.7
MS.gene81101.t1 AT1G27045 51.852 81 38 1 175 254 53 133 1.13e-15 75.9
MS.gene81101.t1 AT3G01220 42.453 106 55 2 177 278 77 180 1.65e-15 75.9
MS.gene81101.t1 AT2G18550 37.671 146 84 2 167 312 40 178 1.73e-15 74.7
MS.gene81101.t1 AT3G01470 45.652 92 50 0 189 280 68 159 2.01e-15 75.5
MS.gene81101.t1 AT5G03790 37.190 121 68 1 184 296 72 192 4.07e-15 73.2
MS.gene81101.t1 AT5G65310 40.909 110 55 2 177 276 57 166 4.11e-15 75.1
MS.gene81101.t1 AT5G65310 40.909 110 55 2 177 276 39 148 4.24e-15 75.1
MS.gene81101.t1 AT2G22430 45.455 99 50 1 185 279 58 156 8.83e-15 74.3
MS.gene81101.t1 AT4G40060 39.669 121 67 2 163 279 35 153 2.31e-14 72.8
MS.gene81101.t1 AT4G36740 43.011 93 52 1 183 275 51 142 1.45e-12 66.6
MS.gene81101.t1 AT4G36740 43.011 93 52 1 183 275 50 141 1.53e-12 66.2
MS.gene81101.t1 AT2G46680 40.659 91 54 0 184 274 27 117 2.24e-12 66.6
MS.gene81101.t1 AT2G46680 40.659 91 54 0 184 274 27 117 2.52e-12 66.6

Find 64 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTACCTTGCTCTTCTATTT+TGG 0.134685 2.2:-28036358 None:intergenic
AAAGCCTCTAATTGGATTAA+TGG 0.207556 2.2:+28035003 MS.gene81101:CDS
GACTCTTCAAAGCCTCTAAT+TGG 0.231354 2.2:+28034995 MS.gene81101:CDS
CTTTGTGTAGTACTAATAAT+TGG 0.238752 2.2:-28035091 None:intergenic
GGTAGGAGTTTGAGTGGTTC+AGG 0.247485 2.2:+28035577 MS.gene81101:CDS
AGTTGATGCAATACAGGATT+AGG 0.292885 2.2:-28035193 None:intergenic
AAGAAGATGCTGCACTATTC+GGG 0.307898 2.2:-28035513 None:intergenic
AATTTATTATCAACCGAGTT+TGG 0.329723 2.2:+28035439 MS.gene81101:CDS
GAAGCCTAAGCCAAGTTCTT+TGG 0.340212 2.2:-28035047 None:intergenic
AAAGAAGATGCTGCACTATT+CGG 0.346517 2.2:-28035514 None:intergenic
TTCAACACCACTTTATCAAT+TGG 0.386708 2.2:+28035067 MS.gene81101:CDS
CATCAAGCTTCCAAAGAACT+TGG 0.420365 2.2:+28035037 MS.gene81101:CDS
TGGAGGTTCTTCAAGGGGAT+TGG 0.425919 2.2:+28035459 MS.gene81101:CDS
AGATCAAGTTGATGCAATAC+AGG 0.427277 2.2:-28035199 None:intergenic
CCGAGTTTGGAGGTTCTTCA+AGG 0.430226 2.2:+28035452 MS.gene81101:CDS
CACATAGTTAAAGTAGTTGC+TGG 0.454337 2.2:-28036660 None:intergenic
AAACACTAACAGAAGAGAAT+AGG 0.466193 2.2:+28036571 MS.gene81101:CDS
GCTTCCAAAGAACTTGGCTT+AGG 0.468530 2.2:+28035043 MS.gene81101:CDS
AATTGGACCAATTGATAAAG+TGG 0.476543 2.2:-28035074 None:intergenic
ATGGAGCTAGGTTTAAGCTT+AGG 0.480018 2.2:+28034971 MS.gene81101:CDS
TTCCTCAACTCTCATTCCCA+TGG 0.491801 2.2:+28035224 MS.gene81101:CDS
AAGAAGAAGAAAAGACTCAA+AGG 0.493193 2.2:+28035137 MS.gene81101:CDS
TTTGGAACCAGACTTCTACT+TGG 0.497117 2.2:-28036340 None:intergenic
AATACTAGTGCCACAATCAA+TGG 0.499863 2.2:+28036735 MS.gene81101:CDS
GCTGGTAATTGCATGTTGAA+TGG 0.500603 2.2:-28036642 None:intergenic
AGTATTAGTGACTGAGGAAG+TGG 0.500829 2.2:-28036718 None:intergenic
GCCCTCGCCAAGTAGAAGTC+TGG 0.501753 2.2:+28036333 MS.gene81101:CDS
CGAGTTTGGAGGTTCTTCAA+GGG 0.504410 2.2:+28035453 MS.gene81101:CDS
AAGAAAAGCAGATTGATCCT+TGG 0.505090 2.2:-28035722 None:intergenic
AGAAGAAGAAAAGACTCAAA+GGG 0.508400 2.2:+28035138 MS.gene81101:CDS
TTGCAACACGTTCGCAAGAA+GGG 0.516542 2.2:-28036683 None:intergenic
GTAACACAAGGAAGAAACTT+AGG 0.517327 2.2:+28035695 MS.gene81101:CDS
TGGAATCCTCCTTCTCAAAA+CGG 0.522286 2.2:+28035244 MS.gene81101:CDS
GAGAAAGAAATCATGGAGCT+AGG 0.525164 2.2:+28034959 None:intergenic
GATAACTGTGGTGTTGGTAA+TGG 0.532740 2.2:+28035673 MS.gene81101:CDS
CCTTGAAGAACCTCCAAACT+CGG 0.537882 2.2:-28035452 None:intergenic
TTATTATCAACCGAGTTTGG+AGG 0.547939 2.2:+28035442 MS.gene81101:CDS
AGGAGTTTGAGTGGTTCAGG+AGG 0.550877 2.2:+28035580 MS.gene81101:CDS
ATGTACAGTCGAGGAGGAAG+TGG 0.554028 2.2:+28035553 MS.gene81101:CDS
GTTGCAACACGTTCGCAAGA+AGG 0.555531 2.2:-28036684 None:intergenic
AGTGGTGGTAGGAGTTTGAG+TGG 0.555829 2.2:+28035571 MS.gene81101:CDS
CACTAACAGAAGAGAATAGG+AGG 0.565496 2.2:+28036574 MS.gene81101:CDS
GAAGATGATAACTGTGGTGT+TGG 0.570473 2.2:+28035667 MS.gene81101:CDS
ATGGTGAAGGCTATGATCAA+AGG 0.573415 2.2:+28035623 MS.gene81101:CDS
TTTGAGAAGGAGGATTCCAT+GGG 0.580464 2.2:-28035240 None:intergenic
AACCAGACTTCTACTTGGCG+AGG 0.590253 2.2:-28036335 None:intergenic
GAGTTTGGAGGTTCTTCAAG+GGG 0.603616 2.2:+28035454 MS.gene81101:CDS
AGTTCTTTGGAAGCTTGATG+AGG 0.611059 2.2:-28035034 None:intergenic
ATTGGATGTGAACGATGAGA+TGG 0.615034 2.2:+28035477 MS.gene81101:CDS
TACAGTCGAGGAGGAAGTGG+TGG 0.618589 2.2:+28035556 MS.gene81101:CDS
ATTGTTATTACCATTGATTG+TGG 0.625260 2.2:-28036745 None:intergenic
TTCCATGGGAATGAGAGTTG+AGG 0.625263 2.2:-28035226 None:intergenic
ACCAGACTTCTACTTGGCGA+GGG 0.626726 2.2:-28036334 None:intergenic
GAAGAAACTTAGGCTCTCCA+AGG 0.627212 2.2:+28035705 MS.gene81101:CDS
GTCGAGGAGGAAGTGGTGGT+AGG 0.639483 2.2:+28035560 MS.gene81101:CDS
GGAGTTTGAGTGGTTCAGGA+GGG 0.643496 2.2:+28035581 MS.gene81101:CDS
GGTTCCAAAATAGAAGAGCA+AGG 0.645595 2.2:+28036354 MS.gene81101:CDS
AGAAGATGCTGCACTATTCG+GGG 0.651031 2.2:-28035512 None:intergenic
GGCACTAGTATTAGTGACTG+AGG 0.651032 2.2:-28036724 None:intergenic
GATCTATGCATGTACAGTCG+AGG 0.663378 2.2:+28035544 MS.gene81101:CDS
AGAGAATAGGAGGCTACACA+AGG 0.664591 2.2:+28036584 MS.gene81101:CDS
CTATGCATGTACAGTCGAGG+AGG 0.685551 2.2:+28035547 MS.gene81101:CDS
AGTGATGAAGATGATAACTG+TGG 0.745381 2.2:+28035661 MS.gene81101:CDS
GTGTTGGTAATGGTAACACA+AGG 0.792389 2.2:+28035683 MS.gene81101:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAATAAAACAAATAAA+TGG - chr2.2:28036227-28036246 None:intergenic 10.0%
!! ACAAAATAAAACAAATAAAT+GGG - chr2.2:28036226-28036245 None:intergenic 10.0%
!! AAGAGTAAAAGATAAAATAT+TGG - chr2.2:28035295-28035314 None:intergenic 15.0%
!! AATATTCTATGTAATGTTAT+TGG + chr2.2:28036009-28036028 MS.gene81101:intron 15.0%
!! ATATCTCTAATATATAGTAT+TGG + chr2.2:28036052-28036071 MS.gene81101:intron 15.0%
!! CTATATATTAGAGATATATT+TGG - chr2.2:28036049-28036068 None:intergenic 15.0%
!!! ATAGAATATTTTCTTTTGAA+AGG - chr2.2:28035999-28036018 None:intergenic 15.0%
!!! CAACTTTATTTTTTAAACTT+TGG + chr2.2:28036448-28036467 MS.gene81101:intron 15.0%
!!! TAAAATATTGGTAACTTTTT+AGG - chr2.2:28035283-28035302 None:intergenic 15.0%
!!! TTGTTTAAAATACTAATAGT+TGG + chr2.2:28036256-28036275 MS.gene81101:intron 15.0%
!! AGTGCTTATTATGATTATAA+GGG + chr2.2:28035393-28035412 MS.gene81101:intron 20.0%
!!! AGATTTTTTTTTTTTATGGC+CGG - chr2.2:28035901-28035920 None:intergenic 20.0%
!!! GTTATTATTGTTTTCACAAT+AGG + chr2.2:28035415-28035434 MS.gene81101:intron 20.0%
!!! TAATCCTATTTTAAAAACAC+TGG - chr2.2:28035362-28035381 None:intergenic 20.0%
! AAAACAAATAAATGGGTCAA+AGG - chr2.2:28036219-28036238 None:intergenic 25.0%
! AAAATTATAGTCAAATGCAG+TGG + chr2.2:28036422-28036441 MS.gene81101:intron 25.0%
! AATTTATTATCAACCGAGTT+TGG + chr2.2:28035439-28035458 MS.gene81101:CDS 25.0%
! AGTGAAAAAAAACACATACA+GGG - chr2.2:28035780-28035799 None:intergenic 25.0%
! ATAAATTTATGTTCTGATGC+AGG + chr2.2:28036496-28036515 MS.gene81101:intron 25.0%
! CAGTGCTTATTATGATTATA+AGG + chr2.2:28035392-28035411 MS.gene81101:intron 25.0%
! CTTTGTGTAGTACTAATAAT+TGG - chr2.2:28035094-28035113 None:intergenic 25.0%
! GTTTCTACTTTAGATTGAAT+TGG + chr2.2:28036097-28036116 MS.gene81101:intron 25.0%
! TGCATAAAAATATGCAAAGT+CGG + chr2.2:28035865-28035884 MS.gene81101:intron 25.0%
!! ATTGTTATTACCATTGATTG+TGG - chr2.2:28036748-28036767 None:intergenic 25.0%
!! TTTTCCATTAATCCAATTAG+AGG - chr2.2:28035010-28035029 None:intergenic 25.0%
!!! ATTTTTTTTTTTTATGGCCG+GGG - chr2.2:28035899-28035918 None:intergenic 25.0%
!!! GAAACCAGTGTTTTTAAAAT+AGG + chr2.2:28035355-28035374 MS.gene81101:intron 25.0%
!!! GATTTTTTTTTTTTATGGCC+GGG - chr2.2:28035900-28035919 None:intergenic 25.0%
AAACACTAACAGAAGAGAAT+AGG + chr2.2:28036571-28036590 MS.gene81101:CDS 30.0%
AAAGCCTCTAATTGGATTAA+TGG + chr2.2:28035003-28035022 MS.gene81101:CDS 30.0%
AAGAAGAAGAAAAGACTCAA+AGG + chr2.2:28035137-28035156 MS.gene81101:CDS 30.0%
AATTGGACCAATTGATAAAG+TGG - chr2.2:28035077-28035096 None:intergenic 30.0%
AGAAGAAGAAAAGACTCAAA+GGG + chr2.2:28035138-28035157 MS.gene81101:CDS 30.0%
CATAAAAATATGCAAAGTCG+GGG + chr2.2:28035867-28035886 MS.gene81101:intron 30.0%
GAGTGAAAAAAAACACATAC+AGG - chr2.2:28035781-28035800 None:intergenic 30.0%
GCATAAAAATATGCAAAGTC+GGG + chr2.2:28035866-28035885 MS.gene81101:intron 30.0%
TTCAACACCACTTTATCAAT+TGG + chr2.2:28035067-28035086 MS.gene81101:CDS 30.0%
! AACAAGAGAGAATTTTCTGA+TGG + chr2.2:28035604-28035623 MS.gene81101:CDS 30.0%
!! ATTGGTAACTTTTTAGGAAG+TGG - chr2.2:28035277-28035296 None:intergenic 30.0%
!! TTGGTAACTTTTTAGGAAGT+GGG - chr2.2:28035276-28035295 None:intergenic 30.0%
AAAGAAGATGCTGCACTATT+CGG - chr2.2:28035517-28035536 None:intergenic 35.0%
AAGAAAAGCAGATTGATCCT+TGG - chr2.2:28035725-28035744 None:intergenic 35.0%
AATACTAGTGCCACAATCAA+TGG + chr2.2:28036735-28036754 MS.gene81101:CDS 35.0%
AGTGATGAAGATGATAACTG+TGG + chr2.2:28035661-28035680 MS.gene81101:CDS 35.0%
CACATAGTTAAAGTAGTTGC+TGG - chr2.2:28036663-28036682 None:intergenic 35.0%
CTAATAGTTGGACACAATTG+TGG + chr2.2:28036268-28036287 MS.gene81101:intron 35.0%
GTAACACAAGGAAGAAACTT+AGG + chr2.2:28035695-28035714 MS.gene81101:CDS 35.0%
TAGTATGTGTGGTATGAATC+TGG - chr2.2:28035937-28035956 None:intergenic 35.0%
TATCGACATGTTAGTATGTG+TGG - chr2.2:28035948-28035967 None:intergenic 35.0%
TGTGGTGTAGAAACAAAAAC+TGG + chr2.2:28036286-28036305 MS.gene81101:intron 35.0%
TTATTATCAACCGAGTTTGG+AGG + chr2.2:28035442-28035461 MS.gene81101:CDS 35.0%
! AGTTGATGCAATACAGGATT+AGG - chr2.2:28035196-28035215 None:intergenic 35.0%
! TGTACCTTGCTCTTCTATTT+TGG - chr2.2:28036361-28036380 None:intergenic 35.0%
!! AGAGAATTTTCTGATGGTGA+AGG + chr2.2:28035610-28035629 MS.gene81101:CDS 35.0%
!! AGATCAAGTTGATGCAATAC+AGG - chr2.2:28035202-28035221 None:intergenic 35.0%
AAGAAGATGCTGCACTATTC+GGG - chr2.2:28035516-28035535 None:intergenic 40.0%
AGTATTAGTGACTGAGGAAG+TGG - chr2.2:28036721-28036740 None:intergenic 40.0%
ATGGAGCTAGGTTTAAGCTT+AGG + chr2.2:28034971-28034990 MS.gene81101:CDS 40.0%
ATGGTGAAGGCTATGATCAA+AGG + chr2.2:28035623-28035642 MS.gene81101:CDS 40.0%
ATTGGATGTGAACGATGAGA+TGG + chr2.2:28035477-28035496 MS.gene81101:CDS 40.0%
CACTAACAGAAGAGAATAGG+AGG + chr2.2:28036574-28036593 MS.gene81101:CDS 40.0%
CATCAAGCTTCCAAAGAACT+TGG + chr2.2:28035037-28035056 MS.gene81101:CDS 40.0%
GAAGATGATAACTGTGGTGT+TGG + chr2.2:28035667-28035686 MS.gene81101:CDS 40.0%
GACTCTTCAAAGCCTCTAAT+TGG + chr2.2:28034995-28035014 MS.gene81101:CDS 40.0%
GCTGGTAATTGCATGTTGAA+TGG - chr2.2:28036645-28036664 None:intergenic 40.0%
GGTTCCAAAATAGAAGAGCA+AGG + chr2.2:28036354-28036373 MS.gene81101:CDS 40.0%
TGGAATCCTCCTTCTCAAAA+CGG + chr2.2:28035244-28035263 MS.gene81101:CDS 40.0%
TTTGAGAAGGAGGATTCCAT+GGG - chr2.2:28035243-28035262 None:intergenic 40.0%
! AGTTCTTTGGAAGCTTGATG+AGG - chr2.2:28035037-28035056 None:intergenic 40.0%
! TGCAGCATCTTCTTTTCAGA+TGG + chr2.2:28035522-28035541 MS.gene81101:CDS 40.0%
! TTTGGAACCAGACTTCTACT+TGG - chr2.2:28036343-28036362 None:intergenic 40.0%
!! GATAACTGTGGTGTTGGTAA+TGG + chr2.2:28035673-28035692 MS.gene81101:CDS 40.0%
!! GTGTTGGTAATGGTAACACA+AGG + chr2.2:28035683-28035702 MS.gene81101:CDS 40.0%
!! TTTTGAGAAGGAGGATTCCA+TGG - chr2.2:28035244-28035263 None:intergenic 40.0%
!!! TTGTTACCGTTTTGAGAAGG+AGG - chr2.2:28035253-28035272 None:intergenic 40.0%
AGAAGATGCTGCACTATTCG+GGG - chr2.2:28035515-28035534 None:intergenic 45.0%
AGAGAATAGGAGGCTACACA+AGG + chr2.2:28036584-28036603 MS.gene81101:CDS 45.0%
CCTTGAAGAACCTCCAAACT+CGG - chr2.2:28035455-28035474 None:intergenic 45.0%
CGAGTTTGGAGGTTCTTCAA+GGG + chr2.2:28035453-28035472 MS.gene81101:CDS 45.0%
GAAGAAACTTAGGCTCTCCA+AGG + chr2.2:28035705-28035724 MS.gene81101:CDS 45.0%
GAAGCCTAAGCCAAGTTCTT+TGG - chr2.2:28035050-28035069 None:intergenic 45.0%
GAGTTTGGAGGTTCTTCAAG+GGG + chr2.2:28035454-28035473 MS.gene81101:CDS 45.0%
GATCTATGCATGTACAGTCG+AGG + chr2.2:28035544-28035563 MS.gene81101:CDS 45.0%
GCTTCCAAAGAACTTGGCTT+AGG + chr2.2:28035043-28035062 MS.gene81101:CDS 45.0%
GGCACTAGTATTAGTGACTG+AGG - chr2.2:28036727-28036746 None:intergenic 45.0%
TTCCATGGGAATGAGAGTTG+AGG - chr2.2:28035229-28035248 None:intergenic 45.0%
TTCCTCAACTCTCATTCCCA+TGG + chr2.2:28035224-28035243 MS.gene81101:CDS 45.0%
TTGCAACACGTTCGCAAGAA+GGG - chr2.2:28036686-28036705 None:intergenic 45.0%
!!! GGGTTGTTACCGTTTTGAGA+AGG - chr2.2:28035256-28035275 None:intergenic 45.0%
!!! TTATAGATTTTTTTTTTTTA+TGG - chr2.2:28035905-28035924 None:intergenic 5.0%
AACCAGACTTCTACTTGGCG+AGG - chr2.2:28036338-28036357 None:intergenic 50.0%
ACCAGACTTCTACTTGGCGA+GGG - chr2.2:28036337-28036356 None:intergenic 50.0%
AGGAGTTTGAGTGGTTCAGG+AGG + chr2.2:28035580-28035599 MS.gene81101:CDS 50.0%
AGTGGTGGTAGGAGTTTGAG+TGG + chr2.2:28035571-28035590 MS.gene81101:CDS 50.0%
ATGTACAGTCGAGGAGGAAG+TGG + chr2.2:28035553-28035572 MS.gene81101:CDS 50.0%
CCGAGTTTGGAGGTTCTTCA+AGG + chr2.2:28035452-28035471 MS.gene81101:CDS 50.0%
CTATGCATGTACAGTCGAGG+AGG + chr2.2:28035547-28035566 MS.gene81101:CDS 50.0%
GGAGTTTGAGTGGTTCAGGA+GGG + chr2.2:28035581-28035600 MS.gene81101:CDS 50.0%
GGTAGGAGTTTGAGTGGTTC+AGG + chr2.2:28035577-28035596 MS.gene81101:CDS 50.0%
GTTGCAACACGTTCGCAAGA+AGG - chr2.2:28036687-28036706 None:intergenic 50.0%
TGGAGGTTCTTCAAGGGGAT+TGG + chr2.2:28035459-28035478 MS.gene81101:CDS 50.0%
CAAAGTCGGGGTTCAAACCC+CGG + chr2.2:28035879-28035898 MS.gene81101:intron 55.0%
TACAGTCGAGGAGGAAGTGG+TGG + chr2.2:28035556-28035575 MS.gene81101:CDS 55.0%
GCCCTCGCCAAGTAGAAGTC+TGG + chr2.2:28036333-28036352 MS.gene81101:CDS 60.0%
GTCGAGGAGGAAGTGGTGGT+AGG + chr2.2:28035560-28035579 MS.gene81101:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 28034971 28036785 28034971 ID=MS.gene81101
chr2.2 mRNA 28034971 28036785 28034971 ID=MS.gene81101.t1;Parent=MS.gene81101
chr2.2 exon 28034971 28035265 28034971 ID=MS.gene81101.t1.exon1;Parent=MS.gene81101.t1
chr2.2 CDS 28034971 28035265 28034971 ID=cds.MS.gene81101.t1;Parent=MS.gene81101.t1
chr2.2 exon 28035437 28035780 28035437 ID=MS.gene81101.t1.exon2;Parent=MS.gene81101.t1
chr2.2 CDS 28035437 28035780 28035437 ID=cds.MS.gene81101.t1;Parent=MS.gene81101.t1
chr2.2 exon 28036296 28036375 28036296 ID=MS.gene81101.t1.exon3;Parent=MS.gene81101.t1
chr2.2 CDS 28036296 28036375 28036296 ID=cds.MS.gene81101.t1;Parent=MS.gene81101.t1
chr2.2 exon 28036518 28036785 28036518 ID=MS.gene81101.t1.exon4;Parent=MS.gene81101.t1
chr2.2 CDS 28036518 28036785 28036518 ID=cds.MS.gene81101.t1;Parent=MS.gene81101.t1
Gene Sequence

>MS.gene81101

ATGGAGCTAGGTTTAAGCTTAGGAGACTCTTCAAAGCCTCTAATTGGATTAATGGAAAAGCATCCTCATCAAGCTTCCAAAGAACTTGGCTTAGGCTTCAACACCACTTTATCAATTGGTCCAATTATTAGTACTACACAAAGAGATCAACTTCAACAAGAAGAAGAAGAAGAAGAAAAGACTCAAAGGGACATCAACAACAACAGTAATAGAACTGAAAATCCTAATCCTGTATTGCATCAACTTGATCTTCTTCCTCAACTCTCATTCCCATGGAATCCTCCTTCTCAAAACGGTAACAACCCACTTCCTAAAAAGTTACCAATATTTTATCTTTTACTCTTGTTTTTTCTCTTATAAAATTTAACTAGTTATAGTGATGATGAAACCAGTGTTTTTAAAATAGGATTAAAAATAGAATCAGTGCTTATTATGATTATAAGGGTTATTATTGTTTTCACAATAGGTAATTTATTATCAACCGAGTTTGGAGGTTCTTCAAGGGGATTGGATGTGAACGATGAGATGGCGCTTTCGTCGTCCCCGAATAGTGCAGCATCTTCTTTTCAGATGGATCTATGCATGTACAGTCGAGGAGGAAGTGGTGGTAGGAGTTTGAGTGGTTCAGGAGGGAACAAGAGAGAATTTTCTGATGGTGAAGGCTATGATCAAAGGAATTCTTCAAGAGTTAGTGATGAAGATGATAACTGTGGTGTTGGTAATGGTAACACAAGGAAGAAACTTAGGCTCTCCAAGGATCAATCTGCTTTTCTTGAAGAAAGCTTCAAAGAACATCACACTCTTAACCCTGTATGTGTTTTTTTTCACTCTCATGTGAAGTAAGTATAAATTAGTTTATATTGTCGTGTTCAGCATAACTAAGCTTATGCACGATGCATAAAAATATGCAAAGTCGGGGTTCAAACCCCGGCCATAAAAAAAAAAAATCTATAAAAAATACTTCCAGATTCATACCACACATACTAACATGTCGATATCAATAATAAATCTATATATGTAATGTTCCTTTCAAAAGAAAATATTCTATGTAATGTTATTGGTGTCATAAGAAACACCAAATATATCTCTAATATATAGTATTGGTGTTACAAATGTTTAAATGAATGTTTCTACTTTAGATTGAATTGGAAAAAAAATGAATGTTTAAATTTTAACACAATTTTTTTTATTGTACATTTTTTTGTACTAATTTTGTAATAAGATGAAAATTTTATGACAATTCTTCCTTTGACCCATTTATTTGTTTTATTTTGTTTTTGATTTTTTGTTTAAAATACTAATAGTTGGACACAATTGTGGTGTAGAAACAAAAACTGGCTCTTGCAAAACAACTCAATCTTCGCCCTCGCCAAGTAGAAGTCTGGTTCCAAAATAGAAGAGCAAGGTACAGATACACTTTGCTAATTTTGATAAATCATACTTAGATTAAAAAAATTATAGTCAAATGCAGTGGATACAACTTTATTTTTTAAACTTTGGATATGAAAATATATAATTTAATCATATAAATTTATGTTCTGATGCAGGACTAAGTTGAAACAAACAGAAGTAGATTGTGAATACTTAAAGAGATGCTGTGAAACACTAACAGAAGAGAATAGGAGGCTACACAAGGAACTTCAAGAATTAAGAGCTTTAAAAACTTCAAATCCATTCAACATGCAATTACCAGCAACTACTTTAACTATGTGCCCTTCTTGCGAACGTGTTGCAACAAATTCTACAGCCACTTCCTCAGTCACTAATACTAGTGCCACAATCAATGGTAATAACAATAACAACATTAACCAGTGA
Protein sequence

>MS.gene81101.t1

MELGLSLGDSSKPLIGLMEKHPHQASKELGLGFNTTLSIGPIISTTQRDQLQQEEEEEEKTQRDINNNSNRTENPNPVLHQLDLLPQLSFPWNPPSQNGNLLSTEFGGSSRGLDVNDEMALSSSPNSAASSFQMDLCMYSRGGSGGRSLSGSGGNKREFSDGEGYDQRNSSRVSDEDDNCGVGNGNTRKKLRLSKDQSAFLEESFKEHHTLNPKQKLALAKQLNLRPRQVEVWFQNRRARTKLKQTEVDCEYLKRCCETLTEENRRLHKELQELRALKTSNPFNMQLPATTLTMCPSCERVATNSTATSSVTNTSATINGNNNNNINQ