AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81113

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81113.t1 MTR_2g061690 94.690 339 6 1 10 336 12 350 0.0 667
MS.gene81113.t1 MTR_2g100280 51.014 345 150 4 10 335 14 358 6.04e-121 353
MS.gene81113.t1 MTR_2g078510 32.692 156 88 6 114 265 112 254 2.16e-17 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81113.t1 AT3G27970 71.884 345 82 3 6 335 10 354 0.0 532
MS.gene81113.t1 AT5G40310 71.637 342 82 3 9 335 13 354 0.0 523
MS.gene81113.t1 AT5G40310 71.554 341 82 3 10 335 5 345 0.0 521
MS.gene81113.t1 AT2G48100 46.628 341 152 5 9 335 15 339 3.87e-104 309
MS.gene81113.t1 AT2G48100 46.628 341 152 5 9 335 15 339 3.87e-104 309
MS.gene81113.t1 AT2G48100 46.628 341 152 5 9 335 15 339 3.87e-104 309
MS.gene81113.t1 AT2G48100 46.628 341 152 5 9 335 15 339 3.87e-104 309
MS.gene81113.t1 AT2G48100 46.628 341 152 5 9 335 15 339 3.87e-104 309
MS.gene81113.t1 AT2G48100 46.628 341 152 5 9 335 15 339 3.87e-104 309
MS.gene81113.t1 AT2G48100 44.118 238 109 4 9 232 15 242 1.03e-63 202
MS.gene81113.t1 AT2G48100 44.118 238 109 4 9 232 15 242 1.03e-63 202
MS.gene81113.t1 AT3G15080 36.420 162 91 5 129 286 84 237 4.97e-22 94.4

Find 72 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTTCTATTCTATGATTT+TGG 0.079022 2.3:+32427282 None:intergenic
CAATATTGAATGCAATATTA+AGG 0.242676 2.3:-32429657 MS.gene81113:intron
TTTGTAATGGAGAACCTATT+TGG 0.244094 2.3:-32428275 MS.gene81113:CDS
ATTTCTCTGCATTCTTGTTT+TGG 0.250746 2.3:+32429167 None:intergenic
TGGTGGATCCGGCGAGAGTT+AGG 0.269201 2.3:+32429098 None:intergenic
GCAGATCCTTTGAGTCTCTA+AGG 0.272727 2.3:-32429299 MS.gene81113:CDS
GAGTGAGTTGCTGAGCTTGC+TGG 0.317206 2.3:+32427715 None:intergenic
TCTCTAAGGGAACATCTTAT+AGG 0.339091 2.3:-32429285 MS.gene81113:intron
CTTGACCGAATTGTCCAAAT+AGG 0.349226 2.3:+32428261 None:intergenic
ATTCAAGATTTCCTTTGTAA+TGG 0.379368 2.3:-32428288 MS.gene81113:CDS
TTCCTTTGAACTACTTTCAA+TGG 0.383655 2.3:+32428312 None:intergenic
AACCAACCTGTGGTATTTGC+AGG 0.384345 2.3:-32429329 MS.gene81113:CDS
CGTGATAATTTGTCAATTAG+TGG 0.384940 2.3:-32428540 MS.gene81113:CDS
GAGATATCTAGCATTTGTTC+AGG 0.385415 2.3:+32427185 None:intergenic
TACTTAACACAAGCATATCT+TGG 0.388791 2.3:-32427689 MS.gene81113:CDS
TGCAGGTATGACATTCAAAC+AGG 0.390404 2.3:-32427365 MS.gene81113:intron
CTGTAGTTTGTCACTGGAAT+TGG 0.410776 2.3:+32428381 None:intergenic
GTGATAATTTGTCAATTAGT+GGG 0.430563 2.3:-32428539 MS.gene81113:CDS
AGTTGCACTAGCTTGCAAAA+TGG 0.454487 2.3:-32428499 MS.gene81113:CDS
GGTGGATCCGGCGAGAGTTA+GGG 0.465427 2.3:+32429099 None:intergenic
TTATTTATATAGGGAATATG+TGG 0.486928 2.3:-32428585 MS.gene81113:intron
GCAAAATCTGCACCCTCTGT+TGG 0.489517 2.3:+32429139 None:intergenic
ACAAGTGTGCAGCATGCTAC+AGG 0.490167 2.3:-32429413 MS.gene81113:CDS
GAGAACCTATTTGGACAATT+CGG 0.491314 2.3:-32428266 MS.gene81113:CDS
GTTGATATATACACTTAATC+CGG 0.491465 2.3:+32429058 None:intergenic
TAGAATAGAAGATTACCCTT+TGG 0.492331 2.3:-32427273 MS.gene81113:CDS
TAATATTGCATTCAATATTG+TGG 0.493082 2.3:+32429660 None:intergenic
CAAGCTAGTGCAACTGCTTG+TGG 0.495162 2.3:+32428507 None:intergenic
GCTAGGATTCTTGTGGGTCA+TGG 0.501796 2.3:-32428228 MS.gene81113:CDS
TCATACCTGTAGTTTGTCAC+TGG 0.508450 2.3:+32428375 None:intergenic
AGGTGGAAGTGATGGCTCAT+TGG 0.511373 2.3:-32428472 MS.gene81113:CDS
TAGTTTGTCACTGGAATTGG+TGG 0.521362 2.3:+32428384 None:intergenic
GCTGCAACACAATCATCATA+AGG 0.521954 2.3:+32427332 None:intergenic
ACTTAACACAAGCATATCTT+GGG 0.523405 2.3:-32427688 MS.gene81113:intron
GCTTGCAAAATGGTGGGAGG+TGG 0.524173 2.3:-32428489 MS.gene81113:CDS
CAACAAAAGTGCCAACTCTC+CGG 0.527126 2.3:-32429077 MS.gene81113:CDS
GGTCAAGAGGTGGAAAAGCT+AGG 0.528425 2.3:-32428245 MS.gene81113:CDS
CAGATCCTTTGAGTCTCTAA+GGG 0.533011 2.3:-32429298 MS.gene81113:CDS
TGCACTAGCTTGCAAAATGG+TGG 0.540492 2.3:-32428496 MS.gene81113:CDS
TGACTACTACTGCTGGTGCA+TGG 0.544301 2.3:-32427156 MS.gene81113:CDS
GGAAAAGCTAGGATTCTTGT+GGG 0.547879 2.3:-32428234 MS.gene81113:CDS
ATGTTTCCTGCAAATACCAC+AGG 0.549170 2.3:+32429323 None:intergenic
TGATAATTTGTCAATTAGTG+GGG 0.552475 2.3:-32428538 MS.gene81113:CDS
CAAGGTCTGACTACTACTGC+TGG 0.556630 2.3:-32427163 MS.gene81113:CDS
TGCCATTGAAAGTAGTTCAA+AGG 0.557135 2.3:-32428314 MS.gene81113:CDS
ATGGTGGGAGGTGGAAGTGA+TGG 0.568037 2.3:-32428480 MS.gene81113:CDS
TGGAAAAGCTAGGATTCTTG+TGG 0.568629 2.3:-32428235 MS.gene81113:CDS
AACAAATGCTAGATATCTCA+AGG 0.568637 2.3:-32427181 MS.gene81113:CDS
ATGTTCCCTTAGAGACTCAA+AGG 0.571888 2.3:+32429293 None:intergenic
GCACTAGCTTGCAAAATGGT+GGG 0.572134 2.3:-32428495 MS.gene81113:CDS
CAATTCCAGTGACAAACTAC+AGG 0.573018 2.3:-32428380 MS.gene81113:intron
TTGGTGGAATGACATAGGAA+TGG 0.573587 2.3:+32428400 None:intergenic
TGGAATTGGTGGAATGACAT+AGG 0.577260 2.3:+32428395 None:intergenic
GGCGAGAGTTAGGGCTGTCA+AGG 0.580970 2.3:+32429108 None:intergenic
TTTGTGGATCAGATGCCAAA+GGG 0.581970 2.3:+32427258 None:intergenic
GAGAAATCTTTGCCAACAGA+GGG 0.589429 2.3:-32429151 MS.gene81113:CDS
TGGACAATTCGGTCAAGAGG+TGG 0.596708 2.3:-32428255 MS.gene81113:CDS
ATACACTTAATCCGGAGAGT+TGG 0.598394 2.3:+32429066 None:intergenic
GATAATTTGTCAATTAGTGG+GGG 0.602354 2.3:-32428537 MS.gene81113:CDS
TTCCTGCAAATACCACAGGT+TGG 0.608858 2.3:+32429327 None:intergenic
ATTTGGACAATTCGGTCAAG+AGG 0.611398 2.3:-32428258 MS.gene81113:CDS
CAACAAAGTGGAGCACCTAG+TGG 0.624003 2.3:-32429385 MS.gene81113:CDS
TTGACAGCCCTAACTCTCGC+CGG 0.624697 2.3:-32429106 MS.gene81113:CDS
ACTCAATTTGCAAAGATGCA+AGG 0.625364 2.3:+32428193 None:intergenic
AGATCTTCATGTGATCCACT+AGG 0.632720 2.3:+32429370 None:intergenic
AAATAGGTTCTCCATTACAA+AGG 0.640704 2.3:+32428277 None:intergenic
AGAGAAATCTTTGCCAACAG+AGG 0.640795 2.3:-32429152 MS.gene81113:CDS
CTACAGGCAGTTCAACAAAG+TGG 0.645951 2.3:-32429397 MS.gene81113:CDS
AGGTTGGTTCATGAGCAGAA+TGG 0.672430 2.3:+32429343 None:intergenic
TCTGCTCATGAACCAACCTG+TGG 0.693667 2.3:-32429339 MS.gene81113:CDS
GCAGGTATGACATTCAAACA+GGG 0.721520 2.3:-32427364 MS.gene81113:intron
CTAGCTTGCAAAATGGTGGG+AGG 0.729911 2.3:-32428492 MS.gene81113:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATTATTCTTATTCTA+AGG - chr2.3:32427236-32427255 MS.gene81113:CDS 10.0%
!! TAAAATTTAGAAACAATTAA+AGG + chr2.3:32428932-32428951 None:intergenic 10.0%
!!! TAGTTTTCATTTAATTAATT+TGG - chr2.3:32427778-32427797 MS.gene81113:intron 10.0%
!! AAAAAAATTATGCATATACA+TGG + chr2.3:32427205-32427224 None:intergenic 15.0%
!! AAAATGAAACTAACATATAT+AGG - chr2.3:32427353-32427372 MS.gene81113:CDS 15.0%
!! ACTCTTGTATTTATTTATAT+AGG - chr2.3:32428217-32428236 MS.gene81113:CDS 15.0%
!! CTCTTGTATTTATTTATATA+GGG - chr2.3:32428218-32428237 MS.gene81113:CDS 15.0%
!! TAAAAAATCAATAACAAAGA+TGG - chr2.3:32428950-32428969 MS.gene81113:intron 15.0%
!!! GTCTTATTATTACAATATAT+AGG - chr2.3:32427375-32427394 MS.gene81113:intron 15.0%
!!! TACAACATTTTTTTTTTTTG+TGG - chr2.3:32429254-32429273 MS.gene81113:intron 15.0%
!!! TACAACTTTTTTTTTTTTTG+TGG - chr2.3:32429201-32429220 MS.gene81113:intron 15.0%
!! ATATATTAATGATGTCTACA+GGG - chr2.3:32429044-32429063 MS.gene81113:intron 20.0%
!! ATATGTGAGATATATGAAAT+TGG + chr2.3:32428874-32428893 None:intergenic 20.0%
!! ATGATAAAGTAGTATATATG+AGG - chr2.3:32428088-32428107 MS.gene81113:intron 20.0%
!! CAATATTGAATGCAATATTA+AGG - chr2.3:32427155-32427174 MS.gene81113:CDS 20.0%
!! TAATATTGCATTCAATATTG+TGG + chr2.3:32427155-32427174 None:intergenic 20.0%
!! TATATATTAATGATGTCTAC+AGG - chr2.3:32429043-32429062 MS.gene81113:intron 20.0%
!! TTATTTATATAGGGAATATG+TGG - chr2.3:32428227-32428246 MS.gene81113:CDS 20.0%
!!! AAAGTGTAAAATGTTTTACA+CGG + chr2.3:32429174-32429193 None:intergenic 20.0%
!!! AAATGTTGTAATTTTTGATC+CGG + chr2.3:32429244-32429263 None:intergenic 20.0%
!!! AACATTTTTTTTTTTTGTGG+TGG - chr2.3:32429257-32429276 MS.gene81113:intron 20.0%
!!! AACTTTTTTTTTTTTTGTGG+TGG - chr2.3:32429204-32429223 MS.gene81113:intron 20.0%
!!! AATCTTCTATTCTATGATTT+TGG + chr2.3:32429533-32429552 None:intergenic 20.0%
!!! ATCTTCTATTCTATGATTTT+GGG + chr2.3:32429532-32429551 None:intergenic 20.0%
!!! ATTAGTCTTATTATATATGC+AGG - chr2.3:32429430-32429449 MS.gene81113:intron 20.0%
!!! GAAAATGATTACCTTATTTT+TGG + chr2.3:32428653-32428672 None:intergenic 20.0%
!!! GTTGTATTTTTAATTATGCA+AGG + chr2.3:32429302-32429321 None:intergenic 20.0%
!!! TTTTTATAAGTTCTTCAAGA+TGG + chr2.3:32427943-32427962 None:intergenic 20.0%
! AACAAATACTAGCTAGAATA+TGG + chr2.3:32428064-32428083 None:intergenic 25.0%
! ATTCAAGATTTCCTTTGTAA+TGG - chr2.3:32428524-32428543 MS.gene81113:CDS 25.0%
! GTTGATATATACACTTAATC+CGG + chr2.3:32427757-32427776 None:intergenic 25.0%
! TTAAATTCAACGATTCTCTA+CGG - chr2.3:32428735-32428754 MS.gene81113:intron 25.0%
! TTAGAGTCACTTATGTATTT+AGG + chr2.3:32427273-32427292 None:intergenic 25.0%
!! AATGTTGTAATTTTTGATCC+GGG + chr2.3:32429243-32429262 None:intergenic 25.0%
!! GTGATAATTTGTCAATTAGT+GGG - chr2.3:32428273-32428292 MS.gene81113:CDS 25.0%
!! TATAATCTGTATGCTTTTCT+AGG - chr2.3:32427615-32427634 MS.gene81113:intron 25.0%
!! TGATAATTTGTCAATTAGTG+GGG - chr2.3:32428274-32428293 MS.gene81113:CDS 25.0%
!!! ATTTTTAATTATGCAAGGTC+CGG + chr2.3:32429297-32429316 None:intergenic 25.0%
!!! TGGACATTGATTTTTTTTTG+TGG + chr2.3:32427296-32427315 None:intergenic 25.0%
!!! TTTTTTTTTTTTGTGGTGGT+CGG - chr2.3:32429208-32429227 MS.gene81113:intron 25.0%
AAATAGGTTCTCCATTACAA+AGG + chr2.3:32428538-32428557 None:intergenic 30.0%
AACAAATGCTAGATATCTCA+AGG - chr2.3:32429631-32429650 MS.gene81113:intron 30.0%
ACTTAACACAAGCATATCTT+GGG - chr2.3:32429124-32429143 MS.gene81113:CDS 30.0%
AGAGAATCGTTGAATTTAAG+AGG + chr2.3:32428734-32428753 None:intergenic 30.0%
AGTATCCAACACCAAAAATA+AGG - chr2.3:32428639-32428658 MS.gene81113:intron 30.0%
CATGCACAGTTTAATACATA+TGG + chr2.3:32427316-32427335 None:intergenic 30.0%
GAGAATCGTTGAATTTAAGA+GGG + chr2.3:32428733-32428752 None:intergenic 30.0%
TACTTAACACAAGCATATCT+TGG - chr2.3:32429123-32429142 MS.gene81113:CDS 30.0%
TAGAATAGAAGATTACCCTT+TGG - chr2.3:32429539-32429558 MS.gene81113:intron 30.0%
TTCCTTTGAACTACTTTCAA+TGG + chr2.3:32428503-32428522 None:intergenic 30.0%
! ACAGAAACAATTTTGCTTCA+TGG - chr2.3:32429577-32429596 MS.gene81113:intron 30.0%
! ATGTTGTAATTTTTGATCCG+GGG + chr2.3:32429242-32429261 None:intergenic 30.0%
! CGTGATAATTTGTCAATTAG+TGG - chr2.3:32428272-32428291 MS.gene81113:CDS 30.0%
! GATAATTTGTCAATTAGTGG+GGG - chr2.3:32428275-32428294 MS.gene81113:CDS 30.0%
! TTTGTAATGGAGAACCTATT+TGG - chr2.3:32428537-32428556 MS.gene81113:CDS 30.0%
!! ATTTCTCTGCATTCTTGTTT+TGG + chr2.3:32427648-32427667 None:intergenic 30.0%
!! TTTTTAATTATGCAAGGTCC+GGG + chr2.3:32429296-32429315 None:intergenic 30.0%
!!! GATTACCTTATTTTTGGTGT+TGG + chr2.3:32428647-32428666 None:intergenic 30.0%
!!! GCAAAATTGTTTCTGTTTTG+TGG + chr2.3:32429573-32429592 None:intergenic 30.0%
!!! TTTTTTTTTTGTGGTGGTCA+GGG - chr2.3:32429263-32429282 MS.gene81113:intron 30.0%
!!! TTTTTTTTTTTGTGGTGGTC+AGG - chr2.3:32429209-32429228 MS.gene81113:intron 30.0%
!!! TTTTTTTTTTTGTGGTGGTC+GGG - chr2.3:32429209-32429228 MS.gene81113:intron 30.0%
ACTCAATTTGCAAAGATGCA+AGG + chr2.3:32428622-32428641 None:intergenic 35.0%
CTGAAAACAGTCTCATGAAT+AGG - chr2.3:32427967-32427986 MS.gene81113:intron 35.0%
GAGAACCTATTTGGACAATT+CGG - chr2.3:32428546-32428565 MS.gene81113:CDS 35.0%
GAGATATCTAGCATTTGTTC+AGG + chr2.3:32429630-32429649 None:intergenic 35.0%
TACCTCATACATCTCAAAGT+TGG - chr2.3:32428807-32428826 MS.gene81113:intron 35.0%
TGACAGATATTCATGCATCA+TGG - chr2.3:32427810-32427829 MS.gene81113:intron 35.0%
TGCCATTGAAAGTAGTTCAA+AGG - chr2.3:32428498-32428517 MS.gene81113:CDS 35.0%
! GAAACAATTTTGCTTCATGG+AGG - chr2.3:32429580-32429599 MS.gene81113:intron 35.0%
! TCTCTAAGGGAACATCTTAT+AGG - chr2.3:32427527-32427546 MS.gene81113:intron 35.0%
!! TTTTAATTATGCAAGGTCCG+GGG + chr2.3:32429295-32429314 None:intergenic 35.0%
!!! TTTTTTTTTTGTGGTGGTCG+GGG - chr2.3:32429210-32429229 MS.gene81113:intron 35.0%
AACTACAGGTATGAGACAAC+AGG - chr2.3:32428446-32428465 MS.gene81113:CDS 40.0%
AATGCAACATCGTATTGCAG+CGG + chr2.3:32428841-32428860 None:intergenic 40.0%
AGAGAAATCTTTGCCAACAG+AGG - chr2.3:32427660-32427679 MS.gene81113:intron 40.0%
AGATCTTCATGTGATCCACT+AGG + chr2.3:32427445-32427464 None:intergenic 40.0%
ATACACTTAATCCGGAGAGT+TGG + chr2.3:32427749-32427768 None:intergenic 40.0%
ATACGATGTTGCATTCGTAG+AGG - chr2.3:32428846-32428865 MS.gene81113:intron 40.0%
ATGTTCCCTTAGAGACTCAA+AGG + chr2.3:32427522-32427541 None:intergenic 40.0%
ATGTTTCCTGCAAATACCAC+AGG + chr2.3:32427492-32427511 None:intergenic 40.0%
ATTTGGACAATTCGGTCAAG+AGG - chr2.3:32428554-32428573 MS.gene81113:CDS 40.0%
CAATTCCAGTGACAAACTAC+AGG - chr2.3:32428432-32428451 MS.gene81113:CDS 40.0%
CAGATCCTTTGAGTCTCTAA+GGG - chr2.3:32427514-32427533 MS.gene81113:intron 40.0%
CTAACACTAGCAAGACTGTT+TGG + chr2.3:32428021-32428040 None:intergenic 40.0%
CTTGACCGAATTGTCCAAAT+AGG + chr2.3:32428554-32428573 None:intergenic 40.0%
GAGAAATCTTTGCCAACAGA+GGG - chr2.3:32427661-32427680 MS.gene81113:intron 40.0%
GCAGGTATGACATTCAAACA+GGG - chr2.3:32429448-32429467 MS.gene81113:intron 40.0%
GCTGCAACACAATCATCATA+AGG + chr2.3:32429483-32429502 None:intergenic 40.0%
GGAAAAGCTAGGATTCTTGT+GGG - chr2.3:32428578-32428597 MS.gene81113:intron 40.0%
TGCAGGTATGACATTCAAAC+AGG - chr2.3:32429447-32429466 MS.gene81113:intron 40.0%
TGGAAAAGCTAGGATTCTTG+TGG - chr2.3:32428577-32428596 MS.gene81113:intron 40.0%
TGGAATTGGTGGAATGACAT+AGG + chr2.3:32428420-32428439 None:intergenic 40.0%
TTGGTGGAATGACATAGGAA+TGG + chr2.3:32428415-32428434 None:intergenic 40.0%
TTTGTGGATCAGATGCCAAA+GGG + chr2.3:32429557-32429576 None:intergenic 40.0%
! AGTTGCACTAGCTTGCAAAA+TGG - chr2.3:32428313-32428332 MS.gene81113:CDS 40.0%
! CTGTAGTTTGTCACTGGAAT+TGG + chr2.3:32428434-32428453 None:intergenic 40.0%
! TAGTTTGTCACTGGAATTGG+TGG + chr2.3:32428431-32428450 None:intergenic 40.0%
! TCATACCTGTAGTTTGTCAC+TGG + chr2.3:32428440-32428459 None:intergenic 40.0%
! TTTTGTGGATCAGATGCCAA+AGG + chr2.3:32429558-32429577 None:intergenic 40.0%
!! GTCCAACTTTGAGATGTATG+AGG + chr2.3:32428812-32428831 None:intergenic 40.0%
!!! ATGTGGTCGCTTTTCAAACT+TGG - chr2.3:32428244-32428263 MS.gene81113:CDS 40.0%
!!! CTGCATTCTTGTTTTGGCAA+TGG + chr2.3:32427642-32427661 None:intergenic 40.0%
!!! TGTGGTCGCTTTTCAAACTT+GGG - chr2.3:32428245-32428264 MS.gene81113:CDS 40.0%
AACCAACCTGTGGTATTTGC+AGG - chr2.3:32427483-32427502 MS.gene81113:intron 45.0%
ACAACTGAGTTAAGCTCACG+AGG - chr2.3:32429317-32429336 MS.gene81113:CDS 45.0%
CTACAGGCAGTTCAACAAAG+TGG - chr2.3:32427415-32427434 MS.gene81113:intron 45.0%
GCAGATCCTTTGAGTCTCTA+AGG - chr2.3:32427513-32427532 MS.gene81113:intron 45.0%
TTCCTGCAAATACCACAGGT+TGG + chr2.3:32427488-32427507 None:intergenic 45.0%
TTGTAGCGTAACTGCAACTG+CGG - chr2.3:32427179-32427198 MS.gene81113:CDS 45.0%
TTGTCAATTAGTGGGGGAAG+AGG - chr2.3:32428281-32428300 MS.gene81113:CDS 45.0%
! AGAGTTGGCACTTTTGTTGG+TGG + chr2.3:32427734-32427753 None:intergenic 45.0%
! AGGTTGGTTCATGAGCAGAA+TGG + chr2.3:32427472-32427491 None:intergenic 45.0%
! GCACTAGCTTGCAAAATGGT+GGG - chr2.3:32428317-32428336 MS.gene81113:CDS 45.0%
! TGCACTAGCTTGCAAAATGG+TGG - chr2.3:32428316-32428335 MS.gene81113:CDS 45.0%
!! CAACAAAAGTGCCAACTCTC+CGG - chr2.3:32427735-32427754 MS.gene81113:CDS 45.0%
!!! AGCTTGCTGGTTTTCATGAG+TGG + chr2.3:32429087-32429106 None:intergenic 45.0%
!!! TGCTGGTTTTCATGAGTGGA+GGG + chr2.3:32429083-32429102 None:intergenic 45.0%
!!! TTGCTGGTTTTCATGAGTGG+AGG + chr2.3:32429084-32429103 None:intergenic 45.0%
ACAAGTGTGCAGCATGCTAC+AGG - chr2.3:32427399-32427418 MS.gene81113:intron 50.0%
ACATCGTATTGCAGCGGTAG+AGG + chr2.3:32428835-32428854 None:intergenic 50.0%
AGGTGGAAGTGATGGCTCAT+TGG - chr2.3:32428340-32428359 MS.gene81113:intron 50.0%
CAAGCTAGTGCAACTGCTTG+TGG + chr2.3:32428308-32428327 None:intergenic 50.0%
CAAGGTCTGACTACTACTGC+TGG - chr2.3:32429649-32429668 MS.gene81113:intron 50.0%
CATCGTATTGCAGCGGTAGA+GGG + chr2.3:32428834-32428853 None:intergenic 50.0%
GCAAAATCTGCACCCTCTGT+TGG + chr2.3:32427676-32427695 None:intergenic 50.0%
GCTAGGATTCTTGTGGGTCA+TGG - chr2.3:32428584-32428603 MS.gene81113:intron 50.0%
GGTCAAGAGGTGGAAAAGCT+AGG - chr2.3:32428567-32428586 MS.gene81113:CDS 50.0%
TCTGCTCATGAACCAACCTG+TGG - chr2.3:32427473-32427492 MS.gene81113:intron 50.0%
TGACTACTACTGCTGGTGCA+TGG - chr2.3:32429656-32429675 MS.gene81113:intron 50.0%
TGGACAATTCGGTCAAGAGG+TGG - chr2.3:32428557-32428576 MS.gene81113:CDS 50.0%
! CAACAAAGTGGAGCACCTAG+TGG - chr2.3:32427427-32427446 MS.gene81113:intron 50.0%
! CGGAGAGTTGGCACTTTTGT+TGG + chr2.3:32427737-32427756 None:intergenic 50.0%
! CTAGCTTGCAAAATGGTGGG+AGG - chr2.3:32428320-32428339 MS.gene81113:CDS 50.0%
!! GGCACTTTTGTTGGTGGATC+CGG + chr2.3:32427728-32427747 None:intergenic 50.0%
CATCATGTGCAGTGCTGCAG+AGG - chr2.3:32428763-32428782 MS.gene81113:intron 55.0%
GAGTGAGTTGCTGAGCTTGC+TGG + chr2.3:32429100-32429119 None:intergenic 55.0%
TTGACAGCCCTAACTCTCGC+CGG - chr2.3:32427706-32427725 MS.gene81113:CDS 55.0%
! ATGGTGGGAGGTGGAAGTGA+TGG - chr2.3:32428332-32428351 MS.gene81113:intron 55.0%
! GCTTGCAAAATGGTGGGAGG+TGG - chr2.3:32428323-32428342 MS.gene81113:CDS 55.0%
GGCGAGAGTTAGGGCTGTCA+AGG + chr2.3:32427707-32427726 None:intergenic 60.0%
GGTGGATCCGGCGAGAGTTA+GGG + chr2.3:32427716-32427735 None:intergenic 60.0%
GGTGGTCAGGGTTTGAACCC+CGG - chr2.3:32429275-32429294 MS.gene81113:intron 60.0%
! TGGTGGATCCGGCGAGAGTT+AGG + chr2.3:32427717-32427736 None:intergenic 60.0%
GGTGGTCGGGGTTTGAACCC+CGG - chr2.3:32429222-32429241 MS.gene81113:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 32427148 32429686 32427148 ID=MS.gene81113
chr2.3 mRNA 32427148 32429686 32427148 ID=MS.gene81113.t1;Parent=MS.gene81113
chr2.3 exon 32429658 32429686 32429658 ID=MS.gene81113.t1.exon1;Parent=MS.gene81113.t1
chr2.3 CDS 32429658 32429686 32429658 ID=cds.MS.gene81113.t1;Parent=MS.gene81113.t1
chr2.3 exon 32429286 32429437 32429286 ID=MS.gene81113.t1.exon2;Parent=MS.gene81113.t1
chr2.3 CDS 32429286 32429437 32429286 ID=cds.MS.gene81113.t1;Parent=MS.gene81113.t1
chr2.3 exon 32429070 32429197 32429070 ID=MS.gene81113.t1.exon3;Parent=MS.gene81113.t1
chr2.3 CDS 32429070 32429197 32429070 ID=cds.MS.gene81113.t1;Parent=MS.gene81113.t1
chr2.3 exon 32428381 32428595 32428381 ID=MS.gene81113.t1.exon4;Parent=MS.gene81113.t1
chr2.3 CDS 32428381 32428595 32428381 ID=cds.MS.gene81113.t1;Parent=MS.gene81113.t1
chr2.3 exon 32428174 32428344 32428174 ID=MS.gene81113.t1.exon5;Parent=MS.gene81113.t1
chr2.3 CDS 32428174 32428344 32428174 ID=cds.MS.gene81113.t1;Parent=MS.gene81113.t1
chr2.3 exon 32427689 32427769 32427689 ID=MS.gene81113.t1.exon6;Parent=MS.gene81113.t1
chr2.3 CDS 32427689 32427769 32427689 ID=cds.MS.gene81113.t1;Parent=MS.gene81113.t1
chr2.3 exon 32427148 32427382 32427148 ID=MS.gene81113.t1.exon7;Parent=MS.gene81113.t1
chr2.3 CDS 32427148 32427382 32427148 ID=cds.MS.gene81113.t1;Parent=MS.gene81113.t1
Gene Sequence

>MS.gene81113

ATGTCCACAATATTGAATGCAATATTAAGGTTTGTAGCGTAACTGCAACTGCGGCCATGTATATGCATAATTTTTTTCTTTACAGAGAATTATATTATTCTTATTCTAAGGAGTATTTCAAACCTAAATACATAAGTGACTCTAACCACAAAAAAAAATCAATGTCCATATGTATTAAACTGTGCATGTGCATCTTAAATTATTTAAAATGAAACTAACATATATAGGTCTTATTATTACAATATATAGGAACAAGTGTGCAGCATGCTACAGGCAGTTCAACAAAGTGGAGCACCTAGTGGATCACATGAAGATCTCTTACCATTCTGCTCATGAACCAACCTGTGGTATTTGCAGGAAACATTGCAGATCCTTTGAGTCTCTAAGGGAACATCTTATAGGTAATTAACATTTTCATAAATATTTATTGATAATAAAGTTGTGACAATCAATACTTAATTAATTAATATAATCTGTATGCTTTTCTAGGTCCATTGCCAAAACAAGAATGCAGAGAAATCTTTGCCAACAGAGGGTGCAGATTTTGCTTGAAAGTCCTTGACAGCCCTAACTCTCGCCGGATCCACCAACAAAAGTGCCAACTCTCCGGATTAAGTGTATATATCAACTTAGTTTTCATTTAATTAATTTGGAACTTGAATTGACAGATATTCATGCATCATGGACAGACAGTTTAAATAAGTGTTTGTAACATAAACGTTTATGTTATTTTTATAACAAAAGATAAAATAAAGTCAAACATTTGATGTATACGCTATAAGCTGTCATAAACCATCTTGAAGAACTTATAAAAATAAGCTGAAAACAGTCTCATGAATAGGTCAAATATATGATATTTCAATAAGTTTTCCAAACAGTCTTGCTAGTGTTAGATAAACTCAAATGAACTAATCCATATTCTAGCTAGTATTTGTTGATAATGATAAAGTAGTATATATGAGGTAAAGAGTTACTAAGCAATAGTAACTATTTCATTCAAACATCTAAATTATAGTTATTAAGCTATAGTAACAAGTTAAAGAGTTACTTTTGAAGTAAGTTGATTCTGACTCTTGTATTTATTTATATAGGGAATATGTGGTCGCTTTTCAAACTTGGGAATTCGTGATAATTTGTCAATTAGTGGGGGAAGAGGACCACAAGCAGTTGCACTAGCTTGCAAAATGGTGGGAGGTGGAAGTGATGGCTCATTGGATATATGTGCAAGAGTTTGCTTAATAGATGAATATGAGAATATCATCTTCCATTCCTATGTCATTCCACCAATTCCAGTGACAAACTACAGGTATGAGACAACAGGTATTAGACATGAATATTTAAGAGATGCAATGCCATTGAAAGTAGTTCAAAGGAAGATTCAAGATTTCCTTTGTAATGGAGAACCTATTTGGACAATTCGGTCAAGAGGTGGAAAAGCTAGGATTCTTGTGGGTCATGGTTTAGATCATGACCTTGCATCTTTGCAAATTGAGTATCCAACACCAAAAATAAGGTAATCATTTTCATTATTACATACTTGAGTTCAGCTAGCGTCTTTTAAGTTGAATGAGTTTTAAATTTTCCCTCTTAAATTCAACGATTCTCTACGGTAATGCATCATGTGCAGTGCTGCAGAGGATCTAAAAGTCGCTAAGTTGCTACCTCATACATCTCAAAGTTGGACCCTCTACCGCTGCAATACGATGTTGCATTCGTAGAGGATCCAATTTCATATATCTCACATATTATAATATATGTTCATAAATTGAGAAACACTTGCACCTTTAATTGTTTCTAAATTTTAAAAAATCAATAACAAAGATGGATGACATAGATCAAATAGAGCTGCAAAAATCAACAATTCATTAAATAAATATATATTGACAATGAATTTGTATATATTAATGATGTCTACAGGGACACTGCAAAATACCCTCCACTCATGAAAACCAGCAAGCTCAGCAACTCACTCAAGTACTTAACACAAGCATATCTTGGGTAAGTATAATCTAAATAAAAGTGTCCGTGTAAAACATTTTACACTTTCAAAAATTACAACTTTTTTTTTTTTTGTGGTGGTCGGGGTTTGAACCCCGGATCAAAAATTACAACATTTTTTTTTTTTGTGGTGGTCAGGGTTTGAACCCCGGACCTTGCATAATTAAAAATACAACTGAGTTAAGCTCACGAGGACTTCAAAAATTACAACTAATCGTACATACGACTTTTAAAATAGTCATAATTAAAATAAAATAAATTTAATGATGTGACGACTATAATTGATTAGTCTTATTATATATGCAGGTATGACATTCAAACAGGGATACAAGATCCTTATGATGATTGTGTTGCAGCAATGAGACTCTACATGAGAATGAGAACCCAAAATCATAGAATAGAAGATTACCCTTTGGCATCTGATCCACAAAACAGAAACAATTTTGCTTCATGGAGGCAAAGTGAGCTTGAAAGAATGAGTCCTGAACAAATGCTAGATATCTCAAGGTCTGACTACTACTGCTGGTGCATGGATCCATGA
Protein sequence

>MS.gene81113.t1

MSTILNAILRNKCAACYRQFNKVEHLVDHMKISYHSAHEPTCGICRKHCRSFESLREHLIGPLPKQECREIFANRGCRFCLKVLDSPNSRRIHQQKCQLSGLSGICGRFSNLGIRDNLSISGGRGPQAVALACKMVGGGSDGSLDICARVCLIDEYENIIFHSYVIPPIPVTNYRDAMPLKVVQRKIQDFLCNGEPIWTIRSRGGKARILVGHGLDHDLASLQIEYPTPKIRDTAKYPPLMKTSKLSNSLKYLTQAYLGYDIQTGIQDPYDDCVAAMRLYMRMRTQNHRIEDYPLASDPQNRNNFASWRQSELERMSPEQMLDISRSDYYCWCMDP