AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81204

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81204.t1 MTR_7g005280 83.761 117 17 1 1 117 4 118 7.30e-67 206
MS.gene81204.t1 MTR_8g099750 66.346 104 33 2 1 103 4 106 1.34e-42 143
MS.gene81204.t1 MTR_3g098810 66.316 95 30 2 1 94 4 97 9.74e-40 134
MS.gene81204.t1 MTR_3g098810 66.316 95 30 2 1 94 4 97 6.00e-39 134
MS.gene81204.t1 MTR_2g080010 61.702 94 33 1 12 102 15 108 2.63e-33 122
MS.gene81204.t1 MTR_7g070140 49.375 160 36 6 57 208 12 134 2.82e-33 116
MS.gene81204.t1 MTR_4g089135 61.053 95 34 1 11 102 14 108 9.54e-33 120
MS.gene81204.t1 MTR_5g041940 60.204 98 36 1 10 104 1 98 1.28e-32 118
MS.gene81204.t1 MTR_7g085220 56.701 97 39 1 9 102 5 101 1.76e-32 120
MS.gene81204.t1 MTR_4g081870 61.458 96 34 1 12 104 9 104 2.71e-32 118
MS.gene81204.t1 MTR_2g093810 56.566 99 40 1 12 107 14 112 6.22e-32 118
MS.gene81204.t1 MTR_3g088110 58.511 94 36 1 9 99 4 97 9.64e-31 114
MS.gene81204.t1 MTR_7g100990 50.980 102 47 1 7 105 6 107 2.68e-30 114
MS.gene81204.t1 MTR_2157s0010 55.319 94 39 1 12 102 8 101 6.28e-29 108
MS.gene81204.t1 MTR_3g064580 55.789 95 37 1 10 99 4 98 1.64e-28 112
MS.gene81204.t1 MTR_5g076850 51.000 100 44 1 10 104 4 103 2.08e-27 108
MS.gene81204.t1 MTR_8g094580 53.684 95 41 1 8 99 3 97 3.09e-27 105
MS.gene81204.t1 MTR_6g084430 53.191 94 41 1 9 99 2 95 4.17e-27 105
MS.gene81204.t1 MTR_2g079990 52.525 99 44 1 9 104 13 111 5.93e-27 105
MS.gene81204.t1 MTR_2g079990 52.000 100 45 1 8 104 12 111 7.32e-27 105
MS.gene81204.t1 MTR_3g435150 51.000 100 46 1 3 99 7 106 8.99e-27 104
MS.gene81204.t1 MTR_8g059170 52.525 99 44 1 9 104 11 109 1.61e-26 103
MS.gene81204.t1 MTR_3g096920 55.670 97 36 3 8 99 3 97 4.16e-26 102
MS.gene81204.t1 MTR_4g108760 48.571 105 51 1 5 106 10 114 5.40e-26 103
MS.gene81204.t1 MTR_7g085260 47.748 111 55 1 10 117 15 125 6.53e-26 103
MS.gene81204.t1 MTR_3g109340 46.429 112 55 2 9 117 19 128 7.01e-26 102
MS.gene81204.t1 MTR_3g109340 46.429 112 55 2 9 117 19 128 7.03e-26 102
MS.gene81204.t1 MTR_3g096920 55.670 97 36 3 8 99 3 97 9.26e-26 102
MS.gene81204.t1 MTR_1g008740 48.673 113 52 3 9 117 10 120 1.34e-25 102
MS.gene81204.t1 MTR_1g008740 48.673 113 52 3 9 117 24 134 1.41e-25 102
MS.gene81204.t1 MTR_2g064090 51.020 98 42 2 12 104 6 102 1.76e-25 101
MS.gene81204.t1 MTR_2g078700 46.429 112 55 2 9 117 20 129 2.43e-25 101
MS.gene81204.t1 MTR_6g012670 49.495 99 47 1 4 99 12 110 2.51e-25 100
MS.gene81204.t1 MTR_3g070030 49.485 97 46 1 6 99 13 109 3.16e-25 100
MS.gene81204.t1 MTR_5g090970 50.926 108 49 3 5 110 12 117 3.37e-25 100
MS.gene81204.t1 MTR_4g098630 48.936 94 43 1 12 100 6 99 4.06e-25 100
MS.gene81204.t1 MTR_2g068920 43.333 120 63 2 2 117 12 130 5.33e-25 99.4
MS.gene81204.t1 MTR_5g014300 46.939 98 47 1 8 100 2 99 5.90e-25 100
MS.gene81204.t1 MTR_5g090970 50.459 109 49 3 5 110 12 118 1.29e-24 98.6
MS.gene81204.t1 MTR_2g068880 44.068 118 61 2 4 117 15 131 1.53e-24 98.6
MS.gene81204.t1 MTR_2g062730 43.925 107 54 1 3 103 1 107 1.94e-24 98.6
MS.gene81204.t1 MTR_8g076110 48.000 100 46 2 12 105 6 105 2.43e-24 97.8
MS.gene81204.t1 MTR_6g477900 49.474 95 43 1 10 99 4 98 3.14e-24 96.3
MS.gene81204.t1 MTR_6g032770 48.980 98 47 1 5 99 11 108 4.96e-24 98.2
MS.gene81204.t1 MTR_5g012080 50.526 95 41 1 11 99 6 100 5.64e-24 97.4
MS.gene81204.t1 MTR_4g101680 50.526 95 41 1 11 99 6 100 5.66e-24 97.8
MS.gene81204.t1 MTR_4g036030 52.632 95 39 2 11 99 6 100 5.77e-24 97.4
MS.gene81204.t1 MTR_5g040420 52.475 101 44 2 5 102 8 107 6.38e-24 97.8
MS.gene81204.t1 MTR_7g011130 50.515 97 45 1 6 99 13 109 6.41e-24 97.4
MS.gene81204.t1 MTR_7g011120 50.515 97 45 1 6 99 2 98 1.07e-23 96.3
MS.gene81204.t1 MTR_4g035590 52.525 99 41 2 12 104 8 106 1.08e-23 97.1
MS.gene81204.t1 MTR_5g021710 48.000 100 46 2 12 105 8 107 1.23e-23 96.7
MS.gene81204.t1 MTR_8g467490 51.546 97 43 2 9 102 12 107 2.24e-23 96.7
MS.gene81204.t1 MTR_6g011860 51.064 94 43 1 12 102 15 108 3.27e-23 95.9
MS.gene81204.t1 MTR_3g116070 48.113 106 50 3 2 103 14 118 1.28e-22 94.0
MS.gene81204.t1 MTR_3g093040 43.434 99 51 1 10 103 106 204 1.46e-22 93.2
MS.gene81204.t1 MTR_1g096430 45.000 100 49 1 12 105 7 106 1.91e-22 93.2
MS.gene81204.t1 MTR_8g024480 42.991 107 55 1 4 104 7 113 6.60e-22 92.8
MS.gene81204.t1 MTR_1g090720 51.613 93 40 1 12 99 42 134 1.32e-21 92.0
MS.gene81204.t1 MTR_1g069805 42.202 109 58 2 12 117 10 116 2.90e-21 89.0
MS.gene81204.t1 MTR_1g097300 46.392 97 43 2 15 104 22 116 1.09e-20 89.7
MS.gene81204.t1 MTR_3g093050 45.161 93 46 1 12 99 22 114 1.13e-20 89.7
MS.gene81204.t1 MTR_3g093050 45.161 93 46 1 12 99 22 114 1.21e-20 89.7
MS.gene81204.t1 MTR_5g069030 44.000 100 51 1 11 105 24 123 1.85e-20 89.0
MS.gene81204.t1 MTR_4g134460 47.872 94 45 2 9 99 52 144 5.43e-20 87.4
MS.gene81204.t1 MTR_2g064470 44.231 104 47 3 9 106 8 106 8.28e-20 85.5
MS.gene81204.t1 MTR_3g070040 42.000 100 58 0 6 105 2 101 1.43e-19 85.5
MS.gene81204.t1 MTR_7g097090 41.284 109 59 2 12 117 10 116 1.45e-19 84.7
MS.gene81204.t1 MTR_8g023840 45.745 94 45 2 12 99 11 104 3.14e-19 85.1
MS.gene81204.t1 MTR_3g096140 41.414 99 49 2 15 106 10 106 6.59e-19 82.8
MS.gene81204.t1 MTR_2g086880 42.105 95 51 1 15 105 21 115 7.93e-19 84.3
MS.gene81204.t1 MTR_2g086880 38.739 111 59 2 4 105 5 115 8.77e-19 84.3
MS.gene81204.t1 MTR_3g096140 41.414 99 49 2 15 106 10 106 2.16e-18 82.4
MS.gene81204.t1 MTR_4g075980 43.878 98 51 2 9 103 13 109 5.04e-17 77.8
MS.gene81204.t1 MTR_0036s0150 44.792 96 47 2 15 104 8 103 5.88e-17 79.0
MS.gene81204.t1 MTR_1g090723 43.750 96 48 2 15 104 11 106 6.49e-17 79.0
MS.gene81204.t1 MTR_1g090723 43.750 96 48 2 15 104 11 106 6.69e-17 79.0
MS.gene81204.t1 MTR_1g090723 43.750 96 48 2 15 104 11 106 6.69e-17 79.0
MS.gene81204.t1 MTR_4g094302 38.298 94 49 2 12 103 11 97 1.21e-16 74.3
MS.gene81204.t1 MTR_4g094302 38.298 94 49 2 12 103 11 97 5.00e-16 73.2
MS.gene81204.t1 MTR_8g023930 42.553 94 48 2 12 99 24 117 5.29e-16 76.3
MS.gene81204.t1 MTR_8g023860 41.053 95 50 2 11 99 11 105 6.99e-16 75.5
MS.gene81204.t1 MTR_8g063550 40.594 101 51 2 11 104 5 103 2.07e-15 73.9
MS.gene81204.t1 MTR_8g063550 40.594 101 51 2 11 104 5 103 2.13e-15 74.3
MS.gene81204.t1 MTR_8g063550 38.679 106 56 2 11 109 5 108 2.16e-15 74.3
MS.gene81204.t1 MTR_8g023900 38.182 110 60 3 14 117 195 302 3.57e-15 73.6
MS.gene81204.t1 MTR_8g023900 34.328 134 73 4 12 139 12 136 7.23e-15 72.8
MS.gene81204.t1 MTR_8g093790 40.860 93 43 3 10 99 8 91 2.19e-14 68.9
MS.gene81204.t1 MTR_8g093580 38.614 101 53 2 11 104 5 103 1.38e-13 68.9
MS.gene81204.t1 MTR_2g086690 37.391 115 58 4 13 117 5 115 4.03e-13 66.6
MS.gene81204.t1 MTR_4g052620 35.088 114 63 4 12 117 5 115 2.53e-12 64.3
MS.gene81204.t1 MTR_3g096140 37.647 85 44 2 29 106 3 85 1.16e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81204.t1 AT5G13180 73.077 104 26 1 1 102 1 104 2.53e-50 164
MS.gene81204.t1 AT2G33480 75.000 96 24 0 4 99 7 102 6.77e-49 160
MS.gene81204.t1 AT2G33480 75.000 96 24 0 4 99 7 102 7.22e-49 160
MS.gene81204.t1 AT3G04070 57.000 100 40 1 3 99 1 100 1.86e-32 120
MS.gene81204.t1 AT1G61110 59.223 103 37 2 8 105 12 114 2.27e-32 119
MS.gene81204.t1 AT3G04070 57.000 100 40 1 3 99 1 100 2.46e-32 120
MS.gene81204.t1 AT1G69490 58.947 95 36 1 12 103 9 103 2.09e-31 115
MS.gene81204.t1 AT3G15510 61.111 90 32 1 16 102 21 110 2.81e-30 114
MS.gene81204.t1 AT1G77450 53.465 101 42 2 3 99 1 100 2.90e-30 112
MS.gene81204.t1 AT1G52880 58.889 90 34 1 16 102 21 110 1.71e-29 111
MS.gene81204.t1 AT1G65910 50.000 102 46 1 10 106 4 105 2.33e-29 114
MS.gene81204.t1 AT5G17260 53.684 95 39 1 10 99 4 98 1.29e-28 111
MS.gene81204.t1 AT3G03200 52.632 95 40 1 10 99 4 98 2.00e-28 111
MS.gene81204.t1 AT5G39610 47.706 109 54 1 1 106 9 117 2.98e-28 107
MS.gene81204.t1 AT1G01720 55.914 93 38 1 10 99 5 97 4.75e-28 107
MS.gene81204.t1 AT3G04060 56.989 93 37 1 10 99 18 110 5.39e-28 108
MS.gene81204.t1 AT4G27410 51.515 99 45 1 9 104 11 109 1.01e-27 106
MS.gene81204.t1 AT1G54330 53.763 93 38 1 12 99 6 98 1.10e-27 106
MS.gene81204.t1 AT5G63790 52.000 100 45 1 3 99 41 140 5.18e-27 105
MS.gene81204.t1 AT5G63790 52.000 100 45 1 3 99 51 150 5.92e-27 105
MS.gene81204.t1 AT5G61430 51.000 100 46 1 3 99 7 106 9.16e-27 104
MS.gene81204.t1 AT3G29035 47.619 105 52 1 4 105 16 120 1.80e-26 103
MS.gene81204.t1 AT5G07680 47.525 101 50 1 9 106 14 114 3.82e-26 103
MS.gene81204.t1 AT2G24430 47.826 115 55 2 6 117 10 122 3.92e-26 102
MS.gene81204.t1 AT2G24430 47.826 115 55 2 6 117 10 122 3.92e-26 102
MS.gene81204.t1 AT1G52890 52.128 94 42 1 9 99 11 104 5.27e-26 102
MS.gene81204.t1 AT5G07680 48.980 98 47 1 12 106 3 100 5.49e-26 102
MS.gene81204.t1 AT5G46590 48.515 101 47 1 8 103 2 102 5.72e-26 101
MS.gene81204.t1 AT5G46590 48.515 101 47 1 8 103 2 102 6.55e-26 101
MS.gene81204.t1 AT5G08790 54.255 94 40 1 9 99 4 97 8.47e-26 101
MS.gene81204.t1 AT5G53950 52.128 94 42 1 12 102 17 110 9.38e-26 102
MS.gene81204.t1 AT5G18270 53.763 93 40 1 10 99 19 111 9.55e-26 102
MS.gene81204.t1 AT3G17730 49.000 100 46 1 10 104 4 103 1.46e-25 99.8
MS.gene81204.t1 AT3G15170 50.515 97 45 1 9 102 17 113 1.51e-25 101
MS.gene81204.t1 AT5G18270 53.763 93 40 1 10 99 19 111 1.62e-25 101
MS.gene81204.t1 AT3G18400 50.515 97 45 1 9 102 2 98 1.94e-25 100
MS.gene81204.t1 AT4G17980 42.609 115 59 2 8 117 2 114 2.17e-25 99.8
MS.gene81204.t1 AT1G33060 49.495 99 44 2 12 105 24 121 2.84e-25 103
MS.gene81204.t1 AT1G33060 49.495 99 44 2 12 105 24 121 2.92e-25 103
MS.gene81204.t1 AT4G17980 42.609 115 59 2 8 117 2 114 3.00e-25 100
MS.gene81204.t1 AT4G27410 44.828 116 44 2 9 104 11 126 7.30e-25 99.4
MS.gene81204.t1 AT3G15500 51.064 94 43 1 9 99 11 104 7.64e-25 99.4
MS.gene81204.t1 AT1G76420 47.959 98 48 1 9 103 19 116 1.95e-24 98.6
MS.gene81204.t1 AT1G79580 48.148 108 49 2 4 104 9 116 2.32e-24 99.0
MS.gene81204.t1 AT1G79580 48.148 108 49 2 4 104 9 116 2.32e-24 99.0
MS.gene81204.t1 AT1G79580 48.148 108 49 2 4 104 9 116 2.32e-24 99.0
MS.gene81204.t1 AT1G79580 48.148 108 49 2 4 104 9 116 2.32e-24 99.0
MS.gene81204.t1 AT1G79580 48.148 108 49 2 4 104 9 116 2.32e-24 99.0
MS.gene81204.t1 AT5G66300 47.222 108 51 2 4 105 4 111 2.94e-24 97.4
MS.gene81204.t1 AT5G62380 49.038 104 43 4 10 105 5 106 4.90e-24 97.8
MS.gene81204.t1 AT5G62380 49.038 104 43 4 10 105 5 106 4.90e-24 97.8
MS.gene81204.t1 AT5G39820 50.505 99 45 2 4 99 13 110 7.03e-24 97.1
MS.gene81204.t1 AT4G36160 47.000 100 47 1 12 105 10 109 7.63e-24 97.8
MS.gene81204.t1 AT4G36160 47.000 100 47 1 12 105 10 109 7.63e-24 97.8
MS.gene81204.t1 AT4G36160 48.000 100 46 2 12 105 14 113 7.79e-24 97.8
MS.gene81204.t1 AT3G10490 51.613 93 40 1 12 99 27 119 7.88e-24 95.1
MS.gene81204.t1 AT2G18060 48.000 100 46 2 12 105 9 108 8.83e-24 97.4
MS.gene81204.t1 AT2G18060 48.000 100 46 2 12 105 9 108 8.83e-24 97.4
MS.gene81204.t1 AT1G33280 50.505 99 43 1 12 104 8 106 9.00e-24 96.3
MS.gene81204.t1 AT4G35580 49.462 93 42 1 12 99 9 101 1.40e-23 98.2
MS.gene81204.t1 AT4G35580 49.462 93 42 1 12 99 9 101 1.45e-23 97.8
MS.gene81204.t1 AT4G35580 49.462 93 42 1 12 99 9 101 1.55e-23 97.8
MS.gene81204.t1 AT3G10480 52.688 93 39 1 12 99 27 119 1.69e-23 97.4
MS.gene81204.t1 AT3G10480 52.688 93 39 1 12 99 27 119 1.70e-23 97.4
MS.gene81204.t1 AT4G10350 51.515 99 42 2 12 104 9 107 2.04e-23 95.9
MS.gene81204.t1 AT1G12260 52.128 94 39 2 12 99 7 100 2.15e-23 95.9
MS.gene81204.t1 AT1G12260 52.128 94 39 2 12 99 7 100 3.77e-23 95.9
MS.gene81204.t1 AT1G62700 53.684 95 38 2 11 99 6 100 4.21e-23 95.9
MS.gene81204.t1 AT1G62700 53.684 95 38 2 11 99 6 100 4.21e-23 95.9
MS.gene81204.t1 AT1G26870 50.495 101 46 2 5 102 10 109 4.48e-23 95.9
MS.gene81204.t1 AT2G46770 44.860 107 53 2 4 104 8 114 5.22e-23 95.1
MS.gene81204.t1 AT3G10500 48.000 100 47 1 12 106 9 108 5.30e-23 96.3
MS.gene81204.t1 AT3G61910 44.118 102 51 2 4 99 3 104 7.67e-23 94.4
MS.gene81204.t1 AT5G14000 43.434 99 55 1 7 104 10 108 9.45e-23 90.1
MS.gene81204.t1 AT3G10490 51.613 93 40 1 12 99 27 119 1.20e-22 95.1
MS.gene81204.t1 AT1G32770 46.465 99 47 1 7 99 11 109 2.00e-22 93.6
MS.gene81204.t1 AT5G04410 48.000 100 47 1 12 106 9 108 2.09e-22 94.7
MS.gene81204.t1 AT1G71930 45.263 95 46 1 12 100 9 103 2.50e-22 92.8
MS.gene81204.t1 AT1G71930 45.263 95 46 1 12 100 9 103 2.50e-22 92.8
MS.gene81204.t1 AT5G14000 43.434 99 55 1 7 104 10 108 5.76e-22 89.4
MS.gene81204.t1 AT1G32510 47.872 94 44 1 12 100 6 99 6.80e-22 90.9
MS.gene81204.t1 AT2G43000 50.000 94 43 2 9 99 16 108 2.14e-21 89.4
MS.gene81204.t1 AT3G12910 50.000 94 42 3 10 99 20 112 1.19e-20 87.8
MS.gene81204.t1 AT3G12977 41.584 101 56 1 9 106 15 115 1.22e-20 87.4
MS.gene81204.t1 AT1G56010 42.157 102 55 2 9 106 16 117 1.26e-20 88.2
MS.gene81204.t1 AT2G17040 45.455 99 50 2 9 104 4 101 1.48e-20 87.0
MS.gene81204.t1 AT1G34190 45.192 104 51 2 6 104 11 113 2.25e-20 89.0
MS.gene81204.t1 AT2G02450 47.727 88 43 1 15 99 54 141 3.73e-20 87.4
MS.gene81204.t1 AT2G02450 47.727 88 43 1 15 99 54 141 4.03e-20 87.8
MS.gene81204.t1 AT5G64060 41.121 107 58 1 8 109 2 108 7.02e-20 86.7
MS.gene81204.t1 AT5G24590 45.161 93 46 1 12 99 13 105 1.01e-19 86.7
MS.gene81204.t1 AT1G34180 46.154 104 50 2 6 104 11 113 1.30e-19 86.7
MS.gene81204.t1 AT1G34180 46.154 104 50 2 6 104 11 113 1.50e-19 86.3
MS.gene81204.t1 AT1G34180 41.379 116 50 2 6 104 11 125 8.73e-19 84.3
MS.gene81204.t1 AT1G34180 41.379 116 50 2 6 104 11 125 1.13e-18 84.0
MS.gene81204.t1 AT2G33480 81.395 43 8 0 57 99 3 45 2.44e-18 80.1
MS.gene81204.t1 AT3G49530 41.935 93 49 1 12 99 13 105 6.90e-18 81.6
MS.gene81204.t1 AT3G49530 41.935 93 49 1 12 99 13 105 7.20e-18 81.3
MS.gene81204.t1 AT5G09330 39.450 109 57 2 8 109 2 108 1.36e-17 80.9
MS.gene81204.t1 AT5G09330 39.450 109 57 2 8 109 2 108 1.36e-17 80.9
MS.gene81204.t1 AT5G09330 39.450 109 57 2 8 109 2 108 1.36e-17 80.9
MS.gene81204.t1 AT5G09330 39.450 109 57 2 8 109 2 108 1.36e-17 80.9
MS.gene81204.t1 AT5G09330 39.450 109 57 2 8 109 2 108 1.36e-17 80.9
MS.gene81204.t1 AT5G64530 39.806 103 51 3 10 110 1 94 1.85e-17 76.3
MS.gene81204.t1 AT4G28530 40.541 111 45 4 11 101 9 118 3.32e-17 78.6
MS.gene81204.t1 AT5G64530 39.806 103 51 3 10 110 1 94 5.23e-17 75.9
MS.gene81204.t1 AT5G04395 39.252 107 49 1 9 99 25 131 5.51e-17 75.9
MS.gene81204.t1 AT4G28530 40.541 111 45 4 11 101 9 118 6.67e-17 78.2
MS.gene81204.t1 AT3G44290 37.895 95 55 1 15 105 17 111 1.28e-16 75.9
MS.gene81204.t1 AT2G27300 36.458 96 57 1 15 106 17 112 5.01e-16 75.5
MS.gene81204.t1 AT3G44290 37.895 95 55 1 15 105 17 111 5.11e-16 75.5
MS.gene81204.t1 AT5G22290 35.789 95 57 1 15 105 24 118 1.09e-15 74.7
MS.gene81204.t1 AT1G02250 38.136 118 66 2 13 123 4 121 2.54e-15 73.9
MS.gene81204.t1 AT1G02230 37.288 118 67 3 13 123 4 121 3.77e-15 73.2
MS.gene81204.t1 AT1G32870 42.478 113 56 3 1 108 1 109 3.05e-14 71.2
MS.gene81204.t1 AT1G32870 42.478 113 56 3 1 108 1 109 3.13e-14 70.9
MS.gene81204.t1 AT1G32870 42.478 113 56 3 1 108 37 145 3.24e-14 70.9
MS.gene81204.t1 AT3G04420 38.136 118 66 3 13 123 4 121 6.80e-13 66.2
MS.gene81204.t1 AT3G04420 38.136 118 66 3 13 123 4 121 8.11e-13 66.6
MS.gene81204.t1 AT5G14000 50.943 53 26 0 7 59 10 62 6.77e-12 63.2
MS.gene81204.t1 AT3G44350 38.043 92 47 3 12 94 5 95 6.24e-11 58.9

Find 47 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCACTCCCATCCTCTTT+TGG 0.255864 5.2:+81961097 MS.gene81204:CDS
GAACTACAAACTCTGGTTAC+TGG 0.339051 5.2:+81960649 MS.gene81204:CDS
TCGATTCCCATTTGGATACT+TGG 0.356013 5.2:-81960619 None:intergenic
TCTTCTCAAGTCTGATCCTT+GGG 0.357339 5.2:+81960354 MS.gene81204:CDS
ATCTTCTCAAGTCTGATCCT+TGG 0.365591 5.2:+81960353 MS.gene81204:CDS
CTGTTAGATCGATTCCCATT+TGG 0.390612 5.2:-81960627 None:intergenic
TTCATGTTCATTTGTGATTG+AGG 0.408967 5.2:-81961059 None:intergenic
TTACTGGAAGGCAACTGGTT+TGG 0.413426 5.2:+81960665 MS.gene81204:CDS
ACAACAAATTCTTCATCTGT+TGG 0.429581 5.2:-81960268 None:intergenic
TGTTCAATATTTGAAGTGTA+AGG 0.430660 5.2:+81960288 MS.gene81204:CDS
TCTGGTTACTGGAAGGCAAC+TGG 0.438388 5.2:+81960660 MS.gene81204:CDS
AAAGAAGCCAAGTATCCAAA+TGG 0.440359 5.2:+81960612 MS.gene81204:CDS
AAGATCAACTTGAGGAATGA+TGG 0.463760 5.2:-81960335 None:intergenic
TTCCTCTCACTCCAACCCCT+TGG 0.484311 5.2:+81960818 MS.gene81204:CDS
CTCAGCTTCAAGTTCGAAAG+TGG 0.494091 5.2:+81960982 MS.gene81204:CDS
TTCATTTGTGATTGAGGAGG+AGG 0.495223 5.2:-81961053 None:intergenic
GAATGATGGAAGCAGGTAAA+GGG 0.498100 5.2:-81960321 None:intergenic
AAGATGACTCATGAAGTTGT+TGG 0.514252 5.2:+81960711 MS.gene81204:CDS
GGTTGGAGTGAGAGGAAGTG+AGG 0.514880 5.2:-81960812 None:intergenic
TAAGCATTTCCAAAAGAGGA+TGG 0.518802 5.2:-81961106 None:intergenic
GGATTAAGCATTTCCAAAAG+AGG 0.521576 5.2:-81961110 None:intergenic
AAATTCAGAGAAAGAAAGAA+AGG 0.524155 5.2:-81960857 None:intergenic
AAGAAGCCAAGTATCCAAAT+GGG 0.526074 5.2:+81960613 MS.gene81204:CDS
GGAATGATGGAAGCAGGTAA+AGG 0.537069 5.2:-81960322 None:intergenic
ACTTGAGGAATGATGGAAGC+AGG 0.539819 5.2:-81960328 None:intergenic
TCTAACAGAACTACAAACTC+TGG 0.548094 5.2:+81960642 MS.gene81204:CDS
GACCAAGGGGTTGGAGTGAG+AGG 0.548262 5.2:-81960820 None:intergenic
CTCATGGTTCAAGAACTGAT+TGG 0.548704 5.2:+81960772 MS.gene81204:CDS
AGAAAGACTGACCAAGGGGT+TGG 0.548887 5.2:-81960829 None:intergenic
AAGCATTTCCAAAAGAGGAT+GGG 0.563423 5.2:-81961105 None:intergenic
TCTGATCCTTGGGATTTACC+TGG 0.570788 5.2:+81960364 MS.gene81204:CDS
GGATGACAACGGAAACCTGC+TGG 0.577662 5.2:-81960247 None:intergenic
TACAGAGGCAAACCACCTCA+TGG 0.581454 5.2:+81960756 MS.gene81204:intron
ATGTTCATTTGTGATTGAGG+AGG 0.585375 5.2:-81961056 None:intergenic
CTTCATCTGTTGGATGACAA+CGG 0.600381 5.2:-81960258 None:intergenic
GAAGAAAGAAAGACTGACCA+AGG 0.608088 5.2:-81960835 None:intergenic
TCAGTTCTTGAACCATGAGG+TGG 0.615010 5.2:-81960768 None:intergenic
TACAAACTCTGGTTACTGGA+AGG 0.624609 5.2:+81960653 MS.gene81204:CDS
CACATATTTCTGAAGAGGAG+AGG 0.625698 5.2:+81960938 MS.gene81204:intron
TATATACCAGGTAAATCCCA+AGG 0.628388 5.2:-81960370 None:intergenic
GACTTGAGAAGATCAACTTG+AGG 0.632069 5.2:-81960343 None:intergenic
GGTGAAGTAAGATTGCCAGC+AGG 0.635653 5.2:+81960232 MS.gene81204:CDS
AAGAAAGAAAGACTGACCAA+GGG 0.651415 5.2:-81960834 None:intergenic
ATTTCTGAAGAGGAGAGGTG+AGG 0.686579 5.2:+81960943 MS.gene81204:intron
TAGGTGATATCGAACAAGAG+AGG 0.689390 5.2:+81960574 MS.gene81204:intron
CAATCAGTTCTTGAACCATG+AGG 0.707588 5.2:-81960771 None:intergenic
AGAAAGAAAGACTGACCAAG+GGG 0.736262 5.2:-81960833 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATTAATATGAATGAATT+GGG + chr5.2:81960886-81960905 MS.gene81204:intron 15.0%
!! TCTTAATTTATAATGAAACA+CGG + chr5.2:81960414-81960433 MS.gene81204:intron 15.0%
!! TGATATTAATATGAATGAAT+TGG + chr5.2:81960885-81960904 MS.gene81204:intron 15.0%
!! TTAAGAAAAAAGAAAAGAAA+AGG - chr5.2:81960400-81960419 None:intergenic 15.0%
! AAATTCAGAGAAAGAAAGAA+AGG - chr5.2:81960860-81960879 None:intergenic 25.0%
!! GGTTTTGCAAAATGAAAATT+GGG + chr5.2:81960907-81960926 MS.gene81204:intron 25.0%
!! TGTTCAATATTTGAAGTGTA+AGG + chr5.2:81960288-81960307 MS.gene81204:CDS 25.0%
!!! GTTGTTTTCTATGATTTCTT+AGG + chr5.2:81961004-81961023 MS.gene81204:CDS 25.0%
ACAACAAATTCTTCATCTGT+TGG - chr5.2:81960271-81960290 None:intergenic 30.0%
TAGACATAGTTATCATGTCT+CGG - chr5.2:81960502-81960521 None:intergenic 30.0%
TTCATGTTCATTTGTGATTG+AGG - chr5.2:81961062-81961081 None:intergenic 30.0%
! ATGGATTTTGTGAAGAAGAA+TGG + chr5.2:81960211-81960230 MS.gene81204:CDS 30.0%
! GGGTTTTGCAAAATGAAAAT+TGG + chr5.2:81960906-81960925 MS.gene81204:intron 30.0%
!!! AAAACTCTTGTTTTCTACAG+AGG + chr5.2:81960741-81960760 MS.gene81204:intron 30.0%
AAAGAAGCCAAGTATCCAAA+TGG + chr5.2:81960612-81960631 MS.gene81204:CDS 35.0%
AAGAAAGAAAGACTGACCAA+GGG - chr5.2:81960837-81960856 None:intergenic 35.0%
AAGAAGCCAAGTATCCAAAT+GGG + chr5.2:81960613-81960632 MS.gene81204:CDS 35.0%
AAGATCAACTTGAGGAATGA+TGG - chr5.2:81960338-81960357 None:intergenic 35.0%
AAGATGACTCATGAAGTTGT+TGG + chr5.2:81960711-81960730 MS.gene81204:CDS 35.0%
AAGCATTTCCAAAAGAGGAT+GGG - chr5.2:81961108-81961127 None:intergenic 35.0%
ATGTTCATTTGTGATTGAGG+AGG - chr5.2:81961059-81961078 None:intergenic 35.0%
TAAGCATTTCCAAAAGAGGA+TGG - chr5.2:81961109-81961128 None:intergenic 35.0%
TATATACCAGGTAAATCCCA+AGG - chr5.2:81960373-81960392 None:intergenic 35.0%
TCTAACAGAACTACAAACTC+TGG + chr5.2:81960642-81960661 MS.gene81204:CDS 35.0%
! AGAAAAGGTGCATATATACC+AGG - chr5.2:81960385-81960404 None:intergenic 35.0%
! TGAGTGTGTGTTGTTCTATT+AGG + chr5.2:81960555-81960574 MS.gene81204:intron 35.0%
! TGTGTCACATATTTCTGAAG+AGG + chr5.2:81960933-81960952 MS.gene81204:intron 35.0%
AGAAAGAAAGACTGACCAAG+GGG - chr5.2:81960836-81960855 None:intergenic 40.0%
ATCTTCTCAAGTCTGATCCT+TGG + chr5.2:81960353-81960372 MS.gene81204:CDS 40.0%
CAATCAGTTCTTGAACCATG+AGG - chr5.2:81960774-81960793 None:intergenic 40.0%
CTCATGGTTCAAGAACTGAT+TGG + chr5.2:81960772-81960791 MS.gene81204:CDS 40.0%
CTTCATCTGTTGGATGACAA+CGG - chr5.2:81960261-81960280 None:intergenic 40.0%
GAACTACAAACTCTGGTTAC+TGG + chr5.2:81960649-81960668 MS.gene81204:CDS 40.0%
GAAGAAAGAAAGACTGACCA+AGG - chr5.2:81960838-81960857 None:intergenic 40.0%
GACTTGAGAAGATCAACTTG+AGG - chr5.2:81960346-81960365 None:intergenic 40.0%
TACAAACTCTGGTTACTGGA+AGG + chr5.2:81960653-81960672 MS.gene81204:CDS 40.0%
TAGGTGATATCGAACAAGAG+AGG + chr5.2:81960574-81960593 MS.gene81204:intron 40.0%
TCGATTCCCATTTGGATACT+TGG - chr5.2:81960622-81960641 None:intergenic 40.0%
TCTTCTCAAGTCTGATCCTT+GGG + chr5.2:81960354-81960373 MS.gene81204:CDS 40.0%
TTCATTTGTGATTGAGGAGG+AGG - chr5.2:81961056-81961075 None:intergenic 40.0%
! CACATATTTCTGAAGAGGAG+AGG + chr5.2:81960938-81960957 MS.gene81204:intron 40.0%
! CTGTTAGATCGATTCCCATT+TGG - chr5.2:81960630-81960649 None:intergenic 40.0%
! GAATGATGGAAGCAGGTAAA+GGG - chr5.2:81960324-81960343 None:intergenic 40.0%
ATTGACATGTCGACACGTGT+TGG + chr5.2:81960457-81960476 MS.gene81204:intron 45.0%
CTACTACATGCAATGTCGTG+TGG + chr5.2:81960525-81960544 MS.gene81204:intron 45.0%
GTCAATGTCGATGTTGTGTC+TGG - chr5.2:81960443-81960462 None:intergenic 45.0%
TCAGTTCTTGAACCATGAGG+TGG - chr5.2:81960771-81960790 None:intergenic 45.0%
TCTGATCCTTGGGATTTACC+TGG + chr5.2:81960364-81960383 MS.gene81204:CDS 45.0%
TTACTGGAAGGCAACTGGTT+TGG + chr5.2:81960665-81960684 MS.gene81204:CDS 45.0%
! AACTCACTCCCATCCTCTTT+TGG + chr5.2:81961097-81961116 MS.gene81204:CDS 45.0%
! ACTTGAGGAATGATGGAAGC+AGG - chr5.2:81960331-81960350 None:intergenic 45.0%
! ATTTCTGAAGAGGAGAGGTG+AGG + chr5.2:81960943-81960962 MS.gene81204:intron 45.0%
! CTCAGCTTCAAGTTCGAAAG+TGG + chr5.2:81960982-81961001 MS.gene81204:CDS 45.0%
! GGAATGATGGAAGCAGGTAA+AGG - chr5.2:81960325-81960344 None:intergenic 45.0%
AGAAAGACTGACCAAGGGGT+TGG - chr5.2:81960832-81960851 None:intergenic 50.0%
GGTGAAGTAAGATTGCCAGC+AGG + chr5.2:81960232-81960251 MS.gene81204:CDS 50.0%
TACAGAGGCAAACCACCTCA+TGG + chr5.2:81960756-81960775 MS.gene81204:intron 50.0%
TCTGGTTACTGGAAGGCAAC+TGG + chr5.2:81960660-81960679 MS.gene81204:CDS 50.0%
!! ACACGTGTTGGTGTCAGACA+TGG + chr5.2:81960469-81960488 MS.gene81204:intron 50.0%
GGATGACAACGGAAACCTGC+TGG - chr5.2:81960250-81960269 None:intergenic 55.0%
GGTTGGAGTGAGAGGAAGTG+AGG - chr5.2:81960815-81960834 None:intergenic 55.0%
TTCCTCTCACTCCAACCCCT+TGG + chr5.2:81960818-81960837 MS.gene81204:CDS 55.0%
GACCAAGGGGTTGGAGTGAG+AGG - chr5.2:81960823-81960842 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 81960211 81961129 81960211 ID=MS.gene81204
chr5.2 mRNA 81960211 81961129 81960211 ID=MS.gene81204.t1;Parent=MS.gene81204
chr5.2 exon 81960211 81960385 81960211 ID=MS.gene81204.t1.exon1;Parent=MS.gene81204.t1
chr5.2 CDS 81960211 81960385 81960211 ID=cds.MS.gene81204.t1;Parent=MS.gene81204.t1
chr5.2 exon 81960577 81960732 81960577 ID=MS.gene81204.t1.exon2;Parent=MS.gene81204.t1
chr5.2 CDS 81960577 81960732 81960577 ID=cds.MS.gene81204.t1;Parent=MS.gene81204.t1
chr5.2 exon 81960761 81960879 81960761 ID=MS.gene81204.t1.exon3;Parent=MS.gene81204.t1
chr5.2 CDS 81960761 81960879 81960761 ID=cds.MS.gene81204.t1;Parent=MS.gene81204.t1
chr5.2 exon 81960953 81961129 81960953 ID=MS.gene81204.t1.exon4;Parent=MS.gene81204.t1
chr5.2 CDS 81960953 81961129 81960953 ID=cds.MS.gene81204.t1;Parent=MS.gene81204.t1
Gene Sequence

>MS.gene81204

ATGGATTTTGTGAAGAAGAATGGTGAAGTAAGATTGCCAGCAGGTTTCCGTTGTCATCCAACAGATGAAGAATTTGTTGTTCAATATTTGAAGTGTAAGGTCTTCTCTTTCCCTTTACCTGCTTCCATCATTCCTCAAGTTGATCTTCTCAAGTCTGATCCTTGGGATTTACCTGGTATATATGCACCTTTTCTTTTCTTTTTTCTTAATTTATAATGAAACACGGACACCAGACACAACATCGACATTGACATGTCGACACGTGTTGGTGTCAGACATGGTCTGATACCGAGACATGATAACTATGTCTACTACTACTACATGCAATGTCGTGTGGATTAATTTGAGTGTGTGTTGTTCTATTAGGTGATATCGAACAAGAGAGGTACTTTTTCAGTACTAAAGAAGCCAAGTATCCAAATGGGAATCGATCTAACAGAACTACAAACTCTGGTTACTGGAAGGCAACTGGTTTGGACAAACAAATCATGAATTCTTCGAAGATGACTCATGAAGTTGTTGGTATGAAGAAAACTCTTGTTTTCTACAGAGGCAAACCACCTCATGGTTCAAGAACTGATTGGATCATGCATGAATATCGCCTCACTTCCTCTCACTCCAACCCCTTGGTCAGTCTTTCTTTCTTCCTTTCTTTCTTTCTCTGAATTTGTAATTGATATTAATATGAATGAATTGGGTTTTGCAAAATGAAAATTGGGTTATGTGTCACATATTTCTGAAGAGGAGAGGTGAGGTGAAGAAGAAGCAGAACTCAGCTTCAAGTTCGAAAGTGGTTGTTTTCTATGATTTCTTAGGAGAGAAGAAGAACAATACTGATGCATCCTCCTCCTCAATCACAAATGAACATGAAAATGAAGACAGCAGTAACTCACTCCCATCCTCTTTTGGAAATGCTTAA
Protein sequence

>MS.gene81204.t1

MDFVKKNGEVRLPAGFRCHPTDEEFVVQYLKCKVFSFPLPASIIPQVDLLKSDPWDLPGDIEQERYFFSTKEAKYPNGNRSNRTTNSGYWKATGLDKQIMNSSKMTHEVVEANHLMVQELIGSCMNIASLPLTPTPWSVFLSSFLSFSEFRRGEVKKKQNSASSSKVVVFYDFLGEKKNNTDASSSSITNEHENEDSSNSLPSSFGNA