AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81215

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81215.t1 MTR_5g097480 98.897 544 6 0 1 544 1 544 0.0 1129
MS.gene81215.t1 MTR_3g056110 57.713 551 217 5 1 544 1 542 0.0 649
MS.gene81215.t1 MTR_5g094450 56.937 555 205 8 1 544 1 532 0.0 620
MS.gene81215.t1 MTR_4g133660 57.350 551 218 8 1 543 1 542 0.0 609
MS.gene81215.t1 MTR_2g082090 63.824 387 137 1 157 543 195 578 0.0 526
MS.gene81215.t1 MTR_2g026250 46.599 588 253 7 12 544 15 596 2.98e-178 515
MS.gene81215.t1 MTR_1g029420 56.216 370 162 0 175 544 223 592 2.13e-159 467
MS.gene81215.t1 MTR_3g089055 54.278 374 171 0 171 544 192 565 5.28e-151 444
MS.gene81215.t1 MTR_3g089055 54.278 374 171 0 171 544 197 570 5.68e-151 445
MS.gene81215.t1 MTR_7g027190 35.603 514 271 9 49 544 202 673 2.99e-87 283
MS.gene81215.t1 MTR_3g065980 36.923 390 209 7 174 544 169 540 2.45e-73 243
MS.gene81215.t1 MTR_7g062120 34.048 373 245 1 173 544 368 740 3.84e-69 236
MS.gene81215.t1 MTR_4g064160 32.796 372 249 1 173 543 312 683 3.39e-65 225
MS.gene81215.t1 MTR_8g442410 33.594 384 234 7 173 544 151 525 6.45e-65 221
MS.gene81215.t1 MTR_4g064150 32.447 376 249 3 173 543 357 732 1.06e-64 224
MS.gene81215.t1 MTR_4g064200 33.245 376 246 4 170 543 277 649 4.77e-64 221
MS.gene81215.t1 MTR_2g097390 29.150 494 310 10 74 543 163 640 1.31e-63 219
MS.gene81215.t1 MTR_7g057230 33.494 415 255 7 140 543 252 656 4.39e-63 218
MS.gene81215.t1 MTR_2g097310 28.279 488 317 9 74 543 165 637 7.28e-62 215
MS.gene81215.t1 MTR_3g022830 31.265 419 228 7 178 544 26 436 9.48e-61 207
MS.gene81215.t1 MTR_6g047750 35.354 396 204 13 175 544 293 662 1.54e-60 211
MS.gene81215.t1 MTR_4g076140 30.787 432 231 7 174 544 45 469 3.28e-60 206
MS.gene81215.t1 MTR_2g097350 31.200 375 249 4 173 543 270 639 5.29e-60 210
MS.gene81215.t1 MTR_4g104020 32.292 384 238 6 174 544 146 520 1.16e-59 206
MS.gene81215.t1 MTR_4g064120 29.904 418 282 4 129 541 212 623 1.53e-59 208
MS.gene81215.t1 MTR_2g097410 31.117 376 252 4 173 543 367 740 2.01e-59 210
MS.gene81215.t1 MTR_2g097467 31.095 402 270 4 143 543 258 653 3.39e-59 208
MS.gene81215.t1 MTR_4g064180 31.903 373 249 3 173 540 250 622 4.45e-59 207
MS.gene81215.t1 MTR_4g097080 30.159 378 251 5 175 544 131 503 5.06e-59 204
MS.gene81215.t1 MTR_5g009080 29.792 433 235 7 174 544 53 478 5.22e-58 201
MS.gene81215.t1 MTR_2g097473 32.976 373 244 5 173 543 285 653 6.18e-58 204
MS.gene81215.t1 MTR_7g074650 34.511 368 220 7 181 544 442 792 1.27e-57 206
MS.gene81215.t1 MTR_2g097463 31.720 372 250 3 173 543 286 654 7.82e-56 199
MS.gene81215.t1 MTR_5g015490 29.592 392 253 8 169 544 106 490 1.01e-51 184
MS.gene81215.t1 MTR_4g102790 32.461 382 237 10 174 543 289 661 1.06e-51 187
MS.gene81215.t1 MTR_1g096030 31.778 450 254 16 127 544 31 459 2.00e-49 177
MS.gene81215.t1 MTR_2g097380 27.197 478 296 11 74 543 127 560 2.40e-49 179
MS.gene81215.t1 MTR_4g077760 32.979 376 237 8 174 544 190 555 7.37e-47 172
MS.gene81215.t1 MTR_4g076020 33.062 369 215 9 181 544 88 429 1.83e-46 169
MS.gene81215.t1 MTR_5g058860 31.008 387 244 10 174 543 116 496 5.97e-44 163
MS.gene81215.t1 MTR_3g072710 29.426 401 250 10 167 543 108 499 3.72e-43 161
MS.gene81215.t1 MTR_2g089100 27.295 403 251 8 174 543 49 442 1.38e-41 155
MS.gene81215.t1 MTR_2g099110 28.974 390 243 9 177 544 74 451 3.87e-40 151
MS.gene81215.t1 MTR_1g069725 30.053 376 240 10 173 543 105 462 7.17e-40 151
MS.gene81215.t1 MTR_2g034250 25.974 385 257 10 178 544 211 585 1.96e-35 140
MS.gene81215.t1 MTR_4g095500 26.794 418 252 9 174 543 56 467 3.37e-34 135
MS.gene81215.t1 MTR_5g015950 25.806 434 238 12 174 543 32 445 3.40e-34 135
MS.gene81215.t1 MTR_3g053270 25.806 434 238 12 174 543 32 445 3.40e-34 135
MS.gene81215.t1 MTR_2g034260 25.882 340 229 8 178 503 211 541 1.99e-30 125
MS.gene81215.t1 MTR_2g034280 24.074 378 270 7 178 544 204 575 2.72e-30 125
MS.gene81215.t1 MTR_4g026485 24.595 370 261 8 178 544 270 624 2.60e-29 122
MS.gene81215.t1 MTR_0092s0100 23.662 355 254 7 193 544 391 731 4.43e-26 113
MS.gene81215.t1 MTR_0092s0100 23.662 355 254 7 193 544 388 728 4.48e-26 113
MS.gene81215.t1 MTR_7g109580 23.770 366 248 10 190 544 209 554 7.76e-26 111
MS.gene81215.t1 MTR_8g093070 28.186 408 249 19 154 543 114 495 1.25e-25 110
MS.gene81215.t1 MTR_1g086970 23.143 350 246 10 208 544 139 478 2.08e-20 94.7
MS.gene81215.t1 MTR_8g020840 25.185 405 243 16 175 544 169 548 4.84e-19 90.9
MS.gene81215.t1 MTR_3g022580 39.286 112 64 2 435 544 74 183 3.28e-18 83.2
MS.gene81215.t1 MTR_3g022005 43.158 95 50 2 452 544 33 125 1.50e-15 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81215.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 704
MS.gene81215.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 704
MS.gene81215.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 704
MS.gene81215.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 704
MS.gene81215.t1 AT1G50600 61.943 494 180 4 56 544 107 597 0.0 619
MS.gene81215.t1 AT1G50600 61.943 494 180 4 56 544 111 601 0.0 619
MS.gene81215.t1 AT1G50600 61.943 494 180 4 56 544 36 526 0.0 617
MS.gene81215.t1 AT2G04890 68.599 414 123 1 131 544 7 413 0.0 596
MS.gene81215.t1 AT4G17230 53.016 547 232 9 1 544 1 525 0.0 559
MS.gene81215.t1 AT4G17230 53.016 547 232 9 1 544 1 525 0.0 559
MS.gene81215.t1 AT1G21450 48.649 444 211 4 118 544 150 593 8.61e-151 445
MS.gene81215.t1 AT1G66350 35.678 398 221 11 158 544 133 506 1.42e-70 235
MS.gene81215.t1 AT3G03450 36.800 375 224 7 174 544 180 545 1.47e-70 236
MS.gene81215.t1 AT1G14920 36.061 391 225 9 163 544 155 529 3.03e-70 234
MS.gene81215.t1 AT2G01570 36.869 396 221 12 160 544 204 581 1.48e-68 231
MS.gene81215.t1 AT1G07530 33.333 375 248 2 171 543 390 764 3.47e-68 234
MS.gene81215.t1 AT5G52510 34.286 385 228 7 175 544 266 640 2.09e-66 227
MS.gene81215.t1 AT5G17490 34.933 375 225 9 174 544 157 516 1.31e-65 222
MS.gene81215.t1 AT5G59450 31.383 376 254 3 173 544 223 598 1.24e-64 222
MS.gene81215.t1 AT2G29060 32.533 375 249 3 173 543 280 654 1.44e-64 223
MS.gene81215.t1 AT2G29060 32.095 377 252 2 171 543 315 691 2.23e-64 223
MS.gene81215.t1 AT2G37650 31.903 373 251 2 173 544 331 701 3.03e-61 214
MS.gene81215.t1 AT2G37650 31.903 373 251 2 173 544 343 713 4.03e-61 214
MS.gene81215.t1 AT3G46600 31.452 372 253 2 174 544 1 371 4.70e-61 206
MS.gene81215.t1 AT1G50420 29.792 433 235 7 174 544 54 479 8.81e-61 208
MS.gene81215.t1 AT3G46600 31.367 373 254 2 173 544 208 579 4.49e-60 209
MS.gene81215.t1 AT3G46600 31.367 373 254 2 173 544 176 547 7.55e-60 207
MS.gene81215.t1 AT2G29065 32.461 382 245 7 173 543 248 627 3.14e-56 199
MS.gene81215.t1 AT3G54220 34.605 367 222 7 181 543 297 649 3.24e-56 200
MS.gene81215.t1 AT1G07520 30.446 381 253 5 173 543 150 528 3.37e-55 194
MS.gene81215.t1 AT1G07520 30.446 381 253 5 173 543 150 528 3.37e-55 194
MS.gene81215.t1 AT1G07520 30.446 381 253 5 173 543 314 692 1.26e-54 196
MS.gene81215.t1 AT1G07520 30.446 381 253 5 173 543 315 693 1.29e-54 196
MS.gene81215.t1 AT5G66770 34.595 370 227 8 181 544 224 584 1.23e-51 186
MS.gene81215.t1 AT5G41920 32.718 379 231 9 173 544 39 400 2.58e-48 173
MS.gene81215.t1 AT3G50650 35.294 357 207 9 200 544 198 542 9.92e-48 174
MS.gene81215.t1 AT4G37650 29.104 402 247 12 170 544 139 529 1.81e-47 173
MS.gene81215.t1 AT1G55580 30.994 342 204 8 226 544 113 445 1.15e-44 164
MS.gene81215.t1 AT1G63100 32.665 349 217 6 174 515 249 586 5.56e-44 165
MS.gene81215.t1 AT1G63100 32.665 349 217 6 174 515 274 611 7.34e-44 165
MS.gene81215.t1 AT4G08250 28.079 406 270 9 147 543 84 476 2.17e-39 150
MS.gene81215.t1 AT3G49950 25.389 386 267 9 174 543 27 407 3.75e-31 125
MS.gene81215.t1 AT2G45160 25.532 376 231 13 191 544 291 639 6.50e-27 115
MS.gene81215.t1 AT3G60630 28.571 301 190 8 256 544 335 622 9.90e-25 108
MS.gene81215.t1 AT3G13840 27.749 382 247 12 175 544 146 510 3.67e-24 106
MS.gene81215.t1 AT4G36710 25.000 380 252 12 178 544 126 485 1.51e-20 95.1
MS.gene81215.t1 AT4G00150 23.098 368 253 11 182 544 212 554 4.28e-20 94.4
MS.gene81215.t1 AT5G67411 33.929 112 72 2 370 481 89 198 1.18e-11 64.7

Find 122 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCAGATGGTGTCGGTTTC+TGG 0.116927 5.2:+81824408 MS.gene81215:CDS
ACTGGCTTCCTTGAGCAATT+TGG 0.182016 5.2:-81824668 None:intergenic
GTTTGCCCTTACTTCAAATT+TGG 0.195361 5.2:+81824576 MS.gene81215:CDS
CAACAAGTGTCACGACTTTC+GGG 0.226734 5.2:-81825025 None:intergenic
CATTTCAAGCGAAGCGAAAT+TGG 0.230694 5.2:-81824262 None:intergenic
CCAGTGGATTTCGTTGATTC+AGG 0.242561 5.2:+81824686 MS.gene81215:CDS
TCAACAAGTGTCACGACTTT+CGG 0.277772 5.2:-81825026 None:intergenic
TCCTTACCCTCTGAGTTCTT+TGG 0.281649 5.2:+81825295 MS.gene81215:CDS
CAATTTCGCTTCGCTTGAAA+TGG 0.314726 5.2:+81824263 MS.gene81215:CDS
CAAGCACAAGCTTAGAGAAT+TGG 0.336532 5.2:+81824173 MS.gene81215:CDS
CAATGTTGAGCAGCATTGTT+TGG 0.339609 5.2:+81825169 MS.gene81215:CDS
CCACCACACATCCGGATTAC+AGG 0.342966 5.2:+81824732 MS.gene81215:CDS
TAGCATGAATGCGAAATTTA+CGG 0.352242 5.2:-81824928 None:intergenic
ATCATTGTCTGCAATTGCTT+TGG 0.368256 5.2:-81824352 None:intergenic
TCGATATTGCGGCAAGCAAT+AGG 0.368619 5.2:-81823872 None:intergenic
AATGGTTGATGGACGAATTA+CGG 0.368703 5.2:+81824388 MS.gene81215:CDS
GTGTCAGCACTCAAAATCAT+AGG 0.371130 5.2:+81824970 MS.gene81215:CDS
TGGTGAACCGATTCAAAGAT+TGG 0.393469 5.2:+81824428 MS.gene81215:CDS
CATAACCTTGCGGTTCGGCC+AGG 0.394232 5.2:+81824894 MS.gene81215:CDS
CTTGAAGCTACTTAAATCTT+CGG 0.395656 5.2:-81824154 None:intergenic
CCGGATGTGTGGTGGTCCCC+CGG 0.395807 5.2:-81824724 None:intergenic
CCTCTGAGTTCTTTGGTGAA+TGG 0.405916 5.2:+81825302 MS.gene81215:CDS
AGCCGAGAATTGAGTTGCTT+GGG 0.407459 5.2:-81823929 None:intergenic
GTTGCACGGTTGTCTGCATC+CGG 0.415709 5.2:+81824474 MS.gene81215:CDS
TGCAGACAATGATTTGCTAA+TGG 0.419025 5.2:+81824362 MS.gene81215:CDS
ATTTGGTTGCTTCTTGTGCT+TGG 0.429491 5.2:+81825414 MS.gene81215:CDS
ATGTAACCAAATTTGAAGTA+AGG 0.431720 5.2:-81824582 None:intergenic
TTTGGTTACATGTCTGCAAA+TGG 0.433675 5.2:+81824594 MS.gene81215:CDS
GTTATGCACGATCCACTCAT+TGG 0.435852 5.2:-81824132 None:intergenic
GCTAATGGCGCAATGGTTGA+TGG 0.437770 5.2:+81824377 MS.gene81215:CDS
TGAGATTCTTGCTGCGACAT+TGG 0.437774 5.2:-81824060 None:intergenic
TTTCACGCTGCAGCTATCTC+TGG 0.438144 5.2:+81824855 MS.gene81215:CDS
GAGCCGAGAATTGAGTTGCT+TGG 0.440934 5.2:-81823930 None:intergenic
AAAGCTTGCTCGGCATTTCA+AGG 0.448893 5.2:+81824821 MS.gene81215:CDS
AGATTTGTAGATTGAACTCC+CGG 0.451937 5.2:-81824493 None:intergenic
GGCGAGCCATAGGTATTGTC+GGG 0.452043 5.2:-81824111 None:intergenic
ACTCCAACGTGCAGTAATAA+AGG 0.452114 5.2:-81823966 None:intergenic
AGCCCAAGCAACTCAATTCT+CGG 0.455091 5.2:+81823927 MS.gene81215:CDS
TTGGAGTCGTCCTTGGCAGC+TGG 0.456230 5.2:+81823982 MS.gene81215:CDS
TGGTTGGATGAATAGAGATT+TGG 0.456807 5.2:+81825397 MS.gene81215:CDS
AACTGCATAACCTTGCGGTT+CGG 0.459814 5.2:+81824889 MS.gene81215:CDS
TCAGCTTATGCCCGTGGAGG+TGG 0.460311 5.2:+81824771 MS.gene81215:CDS
TGAGTGCTGACACTCTCATC+TGG 0.462960 5.2:-81824960 None:intergenic
ATCCTCTCTTTATGCTCTCT+CGG 0.463692 5.2:-81825146 None:intergenic
AGGTCAAGATTTGCAATGGC+TGG 0.465717 5.2:+81825266 MS.gene81215:CDS
TGGCGAGCCATAGGTATTGT+CGG 0.470434 5.2:-81824112 None:intergenic
GAGAGAAGCTCGGCACTCTC+TGG 0.471288 5.2:-81824531 None:intergenic
TATGCTCCCAATCTTTGAAT+CGG 0.477205 5.2:-81824435 None:intergenic
AGAGATGGAGCTCTATATCT+TGG 0.480538 5.2:+81825377 MS.gene81215:CDS
TAGGCTTCAATTTGCTGCAA+TGG 0.480682 5.2:-81823853 None:intergenic
GGTTCGGCCAGGGGAAGCTC+TGG 0.484150 5.2:+81824905 MS.gene81215:CDS
GGTGAACCGATTCAAAGATT+GGG 0.489961 5.2:+81824429 MS.gene81215:CDS
CTGCACGTTGGAGTCGTCCT+TGG 0.491134 5.2:+81823975 MS.gene81215:CDS
CTATCAAGATTATCAGAGTC+AGG 0.491387 5.2:-81824213 None:intergenic
ATAACCTTGCGGTTCGGCCA+GGG 0.491957 5.2:+81824895 MS.gene81215:CDS
GAAATCGATTATATGAACTC+TGG 0.496688 5.2:-81824646 None:intergenic
TAATAAAGGTCAGAAGAAGT+TGG 0.499526 5.2:-81823952 None:intergenic
CATCAGCTGTACAACAATGA+TGG 0.501721 5.2:+81823904 MS.gene81215:CDS
AACAAAATTTCCAGCTGCCA+AGG 0.505944 5.2:-81823992 None:intergenic
CTTCGCTTGAAATGGACAGT+TGG 0.507924 5.2:+81824271 MS.gene81215:CDS
ACCAAAGAACTCAGAGGGTA+AGG 0.509605 5.2:-81825296 None:intergenic
GATCATCAATACCTGTAATC+CGG 0.510867 5.2:-81824743 None:intergenic
GACACGAGCTGCTAGGGAAG+TGG 0.514693 5.2:+81825243 MS.gene81215:CDS
GTGTCACGACTTTCGGGGAT+AGG 0.518113 5.2:-81825019 None:intergenic
TCCACTCATTGGCGAGCCAT+AGG 0.519637 5.2:-81824121 None:intergenic
TGGAACGACACGAGCTGCTA+GGG 0.521228 5.2:+81825237 MS.gene81215:CDS
GTGGAACGACACGAGCTGCT+AGG 0.524981 5.2:+81825236 MS.gene81215:CDS
ATGGAGCTCTATATCTTGGT+TGG 0.526117 5.2:+81825381 MS.gene81215:CDS
TTGCTTTGGCACATGCAGTA+AGG 0.529396 5.2:-81824338 None:intergenic
GTTAACATCATAGCTTGTGA+AGG 0.534917 5.2:+81825203 MS.gene81215:CDS
CGAATTACGGCAGATGGTGT+CGG 0.542149 5.2:+81824401 MS.gene81215:CDS
TGATTTAGATTTCGATATTG+CGG 0.543411 5.2:-81823883 None:intergenic
GCTGCAGCGTGAAACTCAAA+TGG 0.545283 5.2:-81824846 None:intergenic
TGTAACCAAATTTGAAGTAA+GGG 0.545514 5.2:-81824581 None:intergenic
TAACCTTGCGGTTCGGCCAG+GGG 0.545938 5.2:+81824896 MS.gene81215:CDS
ACCTATGGCTCGCCAATGAG+TGG 0.546193 5.2:+81824120 MS.gene81215:CDS
ATCGATTTCCAAATTGCTCA+AGG 0.546444 5.2:+81824660 MS.gene81215:CDS
GCACTCAAAATCATAGGGAT+AGG 0.551891 5.2:+81824976 MS.gene81215:CDS
TTAACATCATAGCTTGTGAA+GGG 0.552742 5.2:+81825204 MS.gene81215:CDS
GGGGAATGATACTTATCAGA+TGG 0.562828 5.2:-81824090 None:intergenic
TGCGACATTGGACTATCGTT+AGG 0.563587 5.2:-81824048 None:intergenic
TTTACGGCCAGAGCTTCCCC+TGG 0.565827 5.2:-81824912 None:intergenic
CCTGAATCAACGAAATCCAC+TGG 0.568290 5.2:-81824686 None:intergenic
GAATTGGAAAATGTAATGCT+TGG 0.570289 5.2:+81824189 MS.gene81215:CDS
TGTCAGCACTCAAAATCATA+GGG 0.577179 5.2:+81824971 MS.gene81215:CDS
CTTCCCCTGGCCGAACCGCA+AGG 0.580073 5.2:-81824899 None:intergenic
TTGCACGGTTGTCTGCATCC+GGG 0.585418 5.2:+81824475 MS.gene81215:CDS
TGTTGGAAGGACTCGTTGCA+CGG 0.585796 5.2:+81824460 MS.gene81215:CDS
TATATGTAGTCCACCTCCAC+GGG 0.589655 5.2:-81824781 None:intergenic
TATAGACTTGAAGAGAGAGA+TGG 0.590094 5.2:+81825362 MS.gene81215:CDS
TTGTGAAGGGGTCGAGAGAG+TGG 0.594827 5.2:+81825217 MS.gene81215:CDS
GACTACATATAGTTGAAAAG+AGG 0.595404 5.2:+81824793 MS.gene81215:CDS
AGAGGTTATCAAAGCTTGCT+CGG 0.596778 5.2:+81824811 MS.gene81215:CDS
AAGATTGGGAGCATACATGT+TGG 0.605912 5.2:+81824443 MS.gene81215:CDS
CTATATGTAGTCCACCTCCA+CGG 0.608482 5.2:-81824782 None:intergenic
TCCATAGTTTCGAGAAAACG+TGG 0.611195 5.2:-81825083 None:intergenic
GTGGAGGTCAAGATTTGCAA+TGG 0.615832 5.2:+81825262 MS.gene81215:CDS
TTGGGAGCATACATGTTGGA+AGG 0.617711 5.2:+81824447 MS.gene81215:CDS
TACGACAGTGCTATCAGCAA+CGG 0.617830 5.2:+81824237 MS.gene81215:CDS
TGACCTTTATTACTGCACGT+TGG 0.620270 5.2:+81823963 MS.gene81215:CDS
GGCGAGAAACTAACAGTCGA+AGG 0.623572 5.2:-81824027 None:intergenic
GATGGACGAATTACGGCAGA+TGG 0.627072 5.2:+81824395 MS.gene81215:CDS
TTACATGAACATACTTTACG+AGG 0.627108 5.2:+81824554 MS.gene81215:CDS
ACGAGCTGCTAGGGAAGTGG+AGG 0.634312 5.2:+81825246 MS.gene81215:CDS
CCACGGGCATAAGCTGATGT+CGG 0.634703 5.2:-81824765 None:intergenic
CATTCACCAAAGAACTCAGA+GGG 0.637813 5.2:-81825301 None:intergenic
CCGACATCAGCTTATGCCCG+TGG 0.638695 5.2:+81824765 MS.gene81215:CDS
ATGATTTGCTAATGGCGCAA+TGG 0.646542 5.2:+81824370 MS.gene81215:CDS
TTCCGAGAGAGCATAAAGAG+AGG 0.651166 5.2:+81825144 MS.gene81215:CDS
GCGAGCCATAGGTATTGTCG+GGG 0.657718 5.2:-81824110 None:intergenic
TGTTCAACTGCATAACCTTG+CGG 0.660074 5.2:+81824884 MS.gene81215:CDS
ACATCAGCTTATGCCCGTGG+AGG 0.663564 5.2:+81824768 MS.gene81215:CDS
TAACATCATAGCTTGTGAAG+GGG 0.668817 5.2:+81825205 MS.gene81215:CDS
CCTGTAATCCGGATGTGTGG+TGG 0.675147 5.2:-81824732 None:intergenic
GGACAGTTGGAGACAAACAA+TGG 0.681512 5.2:+81824284 MS.gene81215:CDS
AACAAGTGTCACGACTTTCG+GGG 0.682943 5.2:-81825024 None:intergenic
GTTCATGTAAGAGAGAAGCT+CGG 0.686963 5.2:-81824541 None:intergenic
AAATTGCTCAAGGAAGCCAG+TGG 0.689859 5.2:+81824670 MS.gene81215:CDS
CCATTCACCAAAGAACTCAG+AGG 0.710837 5.2:-81825302 None:intergenic
ATACCTGTAATCCGGATGTG+TGG 0.719840 5.2:-81824735 None:intergenic
CGAGCCATAGGTATTGTCGG+GGG 0.727707 5.2:-81824109 None:intergenic
AGAATCTAACACAAACACTG+CGG 0.784454 5.2:+81825052 MS.gene81215:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATTTAGATTTCGATATTG+CGG - chr5.2:81823886-81823905 None:intergenic 25.0%
!! ATGTAACCAAATTTGAAGTA+AGG - chr5.2:81824585-81824604 None:intergenic 25.0%
!! TGTAACCAAATTTGAAGTAA+GGG - chr5.2:81824584-81824603 None:intergenic 25.0%
!!! GTTTTCAAGCAATTTCTTTA+TGG - chr5.2:81825330-81825349 None:intergenic 25.0%
CTTGAAGCTACTTAAATCTT+CGG - chr5.2:81824157-81824176 None:intergenic 30.0%
GAAATCGATTATATGAACTC+TGG - chr5.2:81824649-81824668 None:intergenic 30.0%
GAATTGGAAAATGTAATGCT+TGG + chr5.2:81824189-81824208 MS.gene81215:CDS 30.0%
GACTACATATAGTTGAAAAG+AGG + chr5.2:81824793-81824812 MS.gene81215:CDS 30.0%
TAATAAAGGTCAGAAGAAGT+TGG - chr5.2:81823955-81823974 None:intergenic 30.0%
TAGCATGAATGCGAAATTTA+CGG - chr5.2:81824931-81824950 None:intergenic 30.0%
TTACATGAACATACTTTACG+AGG + chr5.2:81824554-81824573 MS.gene81215:CDS 30.0%
! TTAACATCATAGCTTGTGAA+GGG + chr5.2:81825204-81825223 MS.gene81215:CDS 30.0%
AGAATCTAACACAAACACTG+CGG + chr5.2:81825052-81825071 MS.gene81215:CDS 35.0%
AGATTTGTAGATTGAACTCC+CGG - chr5.2:81824496-81824515 None:intergenic 35.0%
ATCATTGTCTGCAATTGCTT+TGG - chr5.2:81824355-81824374 None:intergenic 35.0%
CTATCAAGATTATCAGAGTC+AGG - chr5.2:81824216-81824235 None:intergenic 35.0%
GATCATCAATACCTGTAATC+CGG - chr5.2:81824746-81824765 None:intergenic 35.0%
GTTTGCCCTTACTTCAAATT+TGG + chr5.2:81824576-81824595 MS.gene81215:CDS 35.0%
TATAGACTTGAAGAGAGAGA+TGG + chr5.2:81825362-81825381 MS.gene81215:CDS 35.0%
TATGCTCCCAATCTTTGAAT+CGG - chr5.2:81824438-81824457 None:intergenic 35.0%
TGGTTGGATGAATAGAGATT+TGG + chr5.2:81825397-81825416 MS.gene81215:CDS 35.0%
TGTCAGCACTCAAAATCATA+GGG + chr5.2:81824971-81824990 MS.gene81215:CDS 35.0%
TTTGGTTACATGTCTGCAAA+TGG + chr5.2:81824594-81824613 MS.gene81215:CDS 35.0%
! ATCATAGGGATAGGCTTTTA+AGG + chr5.2:81824985-81825004 MS.gene81215:CDS 35.0%
! ATCGATTTCCAAATTGCTCA+AGG + chr5.2:81824660-81824679 MS.gene81215:CDS 35.0%
! ATTTTTCATGGCTTCTGCAA+TGG - chr5.2:81824622-81824641 None:intergenic 35.0%
! GTTAACATCATAGCTTGTGA+AGG + chr5.2:81825203-81825222 MS.gene81215:CDS 35.0%
! TAACATCATAGCTTGTGAAG+GGG + chr5.2:81825205-81825224 MS.gene81215:CDS 35.0%
!! AACTCTGGCTTCATTTTTCA+TGG - chr5.2:81824634-81824653 None:intergenic 35.0%
!! AATGGTTGATGGACGAATTA+CGG + chr5.2:81824388-81824407 MS.gene81215:CDS 35.0%
!! TGCAGACAATGATTTGCTAA+TGG + chr5.2:81824362-81824381 MS.gene81215:CDS 35.0%
AACAAAATTTCCAGCTGCCA+AGG - chr5.2:81823995-81824014 None:intergenic 40.0%
ACTCCAACGTGCAGTAATAA+AGG - chr5.2:81823969-81823988 None:intergenic 40.0%
AGAGATGGAGCTCTATATCT+TGG + chr5.2:81825377-81825396 MS.gene81215:CDS 40.0%
ATCCTCTCTTTATGCTCTCT+CGG - chr5.2:81825149-81825168 None:intergenic 40.0%
ATGGAGCTCTATATCTTGGT+TGG + chr5.2:81825381-81825400 MS.gene81215:CDS 40.0%
ATTTGGTTGCTTCTTGTGCT+TGG + chr5.2:81825414-81825433 MS.gene81215:CDS 40.0%
CAAGCACAAGCTTAGAGAAT+TGG + chr5.2:81824173-81824192 MS.gene81215:CDS 40.0%
CAATGTTGAGCAGCATTGTT+TGG + chr5.2:81825169-81825188 MS.gene81215:CDS 40.0%
CAATTTCGCTTCGCTTGAAA+TGG + chr5.2:81824263-81824282 MS.gene81215:CDS 40.0%
CATCAGCTGTACAACAATGA+TGG + chr5.2:81823904-81823923 MS.gene81215:CDS 40.0%
CATTCACCAAAGAACTCAGA+GGG - chr5.2:81825304-81825323 None:intergenic 40.0%
CATTTCAAGCGAAGCGAAAT+TGG - chr5.2:81824265-81824284 None:intergenic 40.0%
GCACTCAAAATCATAGGGAT+AGG + chr5.2:81824976-81824995 MS.gene81215:CDS 40.0%
GGGGAATGATACTTATCAGA+TGG - chr5.2:81824093-81824112 None:intergenic 40.0%
GTGTCAGCACTCAAAATCAT+AGG + chr5.2:81824970-81824989 MS.gene81215:CDS 40.0%
GTTCATGTAAGAGAGAAGCT+CGG - chr5.2:81824544-81824563 None:intergenic 40.0%
TCAACAAGTGTCACGACTTT+CGG - chr5.2:81825029-81825048 None:intergenic 40.0%
TCCATAGTTTCGAGAAAACG+TGG - chr5.2:81825086-81825105 None:intergenic 40.0%
TGACCTTTATTACTGCACGT+TGG + chr5.2:81823963-81823982 MS.gene81215:CDS 40.0%
TGTTCAACTGCATAACCTTG+CGG + chr5.2:81824884-81824903 MS.gene81215:CDS 40.0%
! AAGATTGGGAGCATACATGT+TGG + chr5.2:81824443-81824462 MS.gene81215:CDS 40.0%
! AGAGGTTATCAAAGCTTGCT+CGG + chr5.2:81824811-81824830 MS.gene81215:CDS 40.0%
! GGTGAACCGATTCAAAGATT+GGG + chr5.2:81824429-81824448 MS.gene81215:CDS 40.0%
! TCCACGTTTTCTCGAAACTA+TGG + chr5.2:81825082-81825101 MS.gene81215:CDS 40.0%
! TGGTGAACCGATTCAAAGAT+TGG + chr5.2:81824428-81824447 MS.gene81215:CDS 40.0%
!! ATGATTTGCTAATGGCGCAA+TGG + chr5.2:81824370-81824389 MS.gene81215:CDS 40.0%
!! TAGGCTTCAATTTGCTGCAA+TGG - chr5.2:81823856-81823875 None:intergenic 40.0%
!!! TAGGGATAGGCTTTTAAGGT+TGG + chr5.2:81824989-81825008 MS.gene81215:CDS 40.0%
AAATTGCTCAAGGAAGCCAG+TGG + chr5.2:81824670-81824689 MS.gene81215:CDS 45.0%
AACAAGTGTCACGACTTTCG+GGG - chr5.2:81825027-81825046 None:intergenic 45.0%
AACTGCATAACCTTGCGGTT+CGG + chr5.2:81824889-81824908 MS.gene81215:CDS 45.0%
ACCAAAGAACTCAGAGGGTA+AGG - chr5.2:81825299-81825318 None:intergenic 45.0%
AGCCCAAGCAACTCAATTCT+CGG + chr5.2:81823927-81823946 MS.gene81215:CDS 45.0%
AGGTCAAGATTTGCAATGGC+TGG + chr5.2:81825266-81825285 MS.gene81215:CDS 45.0%
ATACCTGTAATCCGGATGTG+TGG - chr5.2:81824738-81824757 None:intergenic 45.0%
CAACAAGTGTCACGACTTTC+GGG - chr5.2:81825028-81825047 None:intergenic 45.0%
CCATTCACCAAAGAACTCAG+AGG - chr5.2:81825305-81825324 None:intergenic 45.0%
CCTCTGAGTTCTTTGGTGAA+TGG + chr5.2:81825302-81825321 MS.gene81215:CDS 45.0%
CCTGAATCAACGAAATCCAC+TGG - chr5.2:81824689-81824708 None:intergenic 45.0%
CTATATGTAGTCCACCTCCA+CGG - chr5.2:81824785-81824804 None:intergenic 45.0%
CTTCGCTTGAAATGGACAGT+TGG + chr5.2:81824271-81824290 MS.gene81215:CDS 45.0%
GGACAGTTGGAGACAAACAA+TGG + chr5.2:81824284-81824303 MS.gene81215:CDS 45.0%
GTGGAGGTCAAGATTTGCAA+TGG + chr5.2:81825262-81825281 MS.gene81215:CDS 45.0%
GTTATGCACGATCCACTCAT+TGG - chr5.2:81824135-81824154 None:intergenic 45.0%
TACGACAGTGCTATCAGCAA+CGG + chr5.2:81824237-81824256 MS.gene81215:CDS 45.0%
TATATGTAGTCCACCTCCAC+GGG - chr5.2:81824784-81824803 None:intergenic 45.0%
TCCTTACCCTCTGAGTTCTT+TGG + chr5.2:81825295-81825314 MS.gene81215:CDS 45.0%
TCGATATTGCGGCAAGCAAT+AGG - chr5.2:81823875-81823894 None:intergenic 45.0%
TGAGATTCTTGCTGCGACAT+TGG - chr5.2:81824063-81824082 None:intergenic 45.0%
TGCGACATTGGACTATCGTT+AGG - chr5.2:81824051-81824070 None:intergenic 45.0%
TTGCTTTGGCACATGCAGTA+AGG - chr5.2:81824341-81824360 None:intergenic 45.0%
! AAAGCTTGCTCGGCATTTCA+AGG + chr5.2:81824821-81824840 MS.gene81215:CDS 45.0%
! ACTGGCTTCCTTGAGCAATT+TGG - chr5.2:81824671-81824690 None:intergenic 45.0%
! AGCCGAGAATTGAGTTGCTT+GGG - chr5.2:81823932-81823951 None:intergenic 45.0%
! TGATTCAGGCTTTTGCAGCT+AGG + chr5.2:81824700-81824719 MS.gene81215:CDS 45.0%
! TTCCGAGAGAGCATAAAGAG+AGG + chr5.2:81825144-81825163 MS.gene81215:CDS 45.0%
! TTGGGAGCATACATGTTGGA+AGG + chr5.2:81824447-81824466 MS.gene81215:CDS 45.0%
!! CCAGTGGATTTCGTTGATTC+AGG + chr5.2:81824686-81824705 MS.gene81215:CDS 45.0%
CATTCCCCCGACAATACCTA+TGG + chr5.2:81824105-81824124 MS.gene81215:CDS 50.0%
CGAATTACGGCAGATGGTGT+CGG + chr5.2:81824401-81824420 MS.gene81215:CDS 50.0%
GATGGACGAATTACGGCAGA+TGG + chr5.2:81824395-81824414 MS.gene81215:CDS 50.0%
GCTAATGGCGCAATGGTTGA+TGG + chr5.2:81824377-81824396 MS.gene81215:CDS 50.0%
GCTGCAGCGTGAAACTCAAA+TGG - chr5.2:81824849-81824868 None:intergenic 50.0%
GGCGAGAAACTAACAGTCGA+AGG - chr5.2:81824030-81824049 None:intergenic 50.0%
TGTTGGAAGGACTCGTTGCA+CGG + chr5.2:81824460-81824479 MS.gene81215:CDS 50.0%
TTTCACGCTGCAGCTATCTC+TGG + chr5.2:81824855-81824874 MS.gene81215:CDS 50.0%
! GAGCCGAGAATTGAGTTGCT+TGG - chr5.2:81823933-81823952 None:intergenic 50.0%
! TGGCGAGCCATAGGTATTGT+CGG - chr5.2:81824115-81824134 None:intergenic 50.0%
!! TGAGTGCTGACACTCTCATC+TGG - chr5.2:81824963-81824982 None:intergenic 50.0%
ACATCAGCTTATGCCCGTGG+AGG + chr5.2:81824768-81824787 MS.gene81215:CDS 55.0%
ACCTATGGCTCGCCAATGAG+TGG + chr5.2:81824120-81824139 MS.gene81215:CDS 55.0%
CCACCACACATCCGGATTAC+AGG + chr5.2:81824732-81824751 MS.gene81215:CDS 55.0%
CCACGGGCATAAGCTGATGT+CGG - chr5.2:81824768-81824787 None:intergenic 55.0%
CCTGTAATCCGGATGTGTGG+TGG - chr5.2:81824735-81824754 None:intergenic 55.0%
GTGTCACGACTTTCGGGGAT+AGG - chr5.2:81825022-81825041 None:intergenic 55.0%
GTTGCACGGTTGTCTGCATC+CGG + chr5.2:81824474-81824493 MS.gene81215:CDS 55.0%
TCCACTCATTGGCGAGCCAT+AGG - chr5.2:81824124-81824143 None:intergenic 55.0%
TGGAACGACACGAGCTGCTA+GGG + chr5.2:81825237-81825256 MS.gene81215:CDS 55.0%
TTGCACGGTTGTCTGCATCC+GGG + chr5.2:81824475-81824494 MS.gene81215:CDS 55.0%
TTGTGAAGGGGTCGAGAGAG+TGG + chr5.2:81825217-81825236 MS.gene81215:CDS 55.0%
! ATAACCTTGCGGTTCGGCCA+GGG + chr5.2:81824895-81824914 MS.gene81215:CDS 55.0%
! CGAGCCATAGGTATTGTCGG+GGG - chr5.2:81824112-81824131 None:intergenic 55.0%
! GCGAGCCATAGGTATTGTCG+GGG - chr5.2:81824113-81824132 None:intergenic 55.0%
! GGCGAGCCATAGGTATTGTC+GGG - chr5.2:81824114-81824133 None:intergenic 55.0%
ACGAGCTGCTAGGGAAGTGG+AGG + chr5.2:81825246-81825265 MS.gene81215:CDS 60.0%
CCGACATCAGCTTATGCCCG+TGG + chr5.2:81824765-81824784 MS.gene81215:CDS 60.0%
CTGCACGTTGGAGTCGTCCT+TGG + chr5.2:81823975-81823994 MS.gene81215:CDS 60.0%
GACACGAGCTGCTAGGGAAG+TGG + chr5.2:81825243-81825262 MS.gene81215:CDS 60.0%
GAGAGAAGCTCGGCACTCTC+TGG - chr5.2:81824534-81824553 None:intergenic 60.0%
GTGGAACGACACGAGCTGCT+AGG + chr5.2:81825236-81825255 MS.gene81215:CDS 60.0%
TCAGCTTATGCCCGTGGAGG+TGG + chr5.2:81824771-81824790 MS.gene81215:CDS 60.0%
TTGGAGTCGTCCTTGGCAGC+TGG + chr5.2:81823982-81824001 MS.gene81215:CDS 60.0%
TTTACGGCCAGAGCTTCCCC+TGG - chr5.2:81824915-81824934 None:intergenic 60.0%
! AGGCTTTTGCAGCTAGGCCC+GGG + chr5.2:81824706-81824725 MS.gene81215:CDS 60.0%
! CAGGCTTTTGCAGCTAGGCC+CGG + chr5.2:81824705-81824724 MS.gene81215:CDS 60.0%
! CATAACCTTGCGGTTCGGCC+AGG + chr5.2:81824894-81824913 MS.gene81215:CDS 60.0%
! TAACCTTGCGGTTCGGCCAG+GGG + chr5.2:81824896-81824915 MS.gene81215:CDS 60.0%
!! CGGCAGATGGTGTCGGTTTC+TGG + chr5.2:81824408-81824427 MS.gene81215:CDS 60.0%
! GCTTTTGCAGCTAGGCCCGG+GGG + chr5.2:81824708-81824727 MS.gene81215:CDS 65.0%
! GGCTTTTGCAGCTAGGCCCG+GGG + chr5.2:81824707-81824726 MS.gene81215:CDS 65.0%
CCGGATGTGTGGTGGTCCCC+CGG - chr5.2:81824727-81824746 None:intergenic 70.0%
CCGGGGGACCACCACACATC+CGG + chr5.2:81824724-81824743 MS.gene81215:CDS 70.0%
CGGATGTGTGGTGGTCCCCC+GGG - chr5.2:81824726-81824745 None:intergenic 70.0%
CTTCCCCTGGCCGAACCGCA+AGG - chr5.2:81824902-81824921 None:intergenic 70.0%
GGTTCGGCCAGGGGAAGCTC+TGG + chr5.2:81824905-81824924 MS.gene81215:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 81823808 81825442 81823808 ID=MS.gene81215
chr5.2 mRNA 81823808 81825442 81823808 ID=MS.gene81215.t1;Parent=MS.gene81215
chr5.2 exon 81823808 81825442 81823808 ID=MS.gene81215.t1.exon1;Parent=MS.gene81215.t1
chr5.2 CDS 81823808 81825442 81823808 ID=cds.MS.gene81215.t1;Parent=MS.gene81215.t1
Gene Sequence

>MS.gene81215

ATGCAAGCATCAGAGCAGCATAGAAGTTCAAGTATGTACTATCAACCATTGCAGCAAATTGAAGCCTATTGCTTGCCGCAATATCGAAATCTAAATCATCAGCTGTACAACAATGATGGAGCCCAAGCAACTCAATTCTCGGCTCCAACTTCTTCTGACCTTTATTACTGCACGTTGGAGTCGTCCTTGGCAGCTGGAAATTTTGTTGTTTACAACTCTCCTTCGACTGTTAGTTTCTCGCCTAACGATAGTCCAATGTCGCAGCAAGAATCTCAGTCTTATCCATCTGATAAGTATCATTCCCCCGACAATACCTATGGCTCGCCAATGAGTGGATCGTGCATAACCGAAGATTTAAGTAGCTTCAAGCACAAGCTTAGAGAATTGGAAAATGTAATGCTTGGTCCTGACTCTGATAATCTTGATAGTTACGACAGTGCTATCAGCAACGGAACCAATTTCGCTTCGCTTGAAATGGACAGTTGGAGACAAACAATGGTAGCAATTTCGAGCAAAAATCTAAAGCACATCCTTACTGCATGTGCCAAAGCAATTGCAGACAATGATTTGCTAATGGCGCAATGGTTGATGGACGAATTACGGCAGATGGTGTCGGTTTCTGGTGAACCGATTCAAAGATTGGGAGCATACATGTTGGAAGGACTCGTTGCACGGTTGTCTGCATCCGGGAGTTCAATCTACAAATCTTTAAGATGCAAAGAACCAGAGAGTGCCGAGCTTCTCTCTTACATGAACATACTTTACGAGGTTTGCCCTTACTTCAAATTTGGTTACATGTCTGCAAATGGAGCCATTGCAGAAGCCATGAAAAATGAAGCCAGAGTTCATATAATCGATTTCCAAATTGCTCAAGGAAGCCAGTGGATTTCGTTGATTCAGGCTTTTGCAGCTAGGCCCGGGGGACCACCACACATCCGGATTACAGGTATTGATGATCCGACATCAGCTTATGCCCGTGGAGGTGGACTACATATAGTTGAAAAGAGGTTATCAAAGCTTGCTCGGCATTTCAAGGTGCCATTTGAGTTTCACGCTGCAGCTATCTCTGGTTGTGATGTTCAACTGCATAACCTTGCGGTTCGGCCAGGGGAAGCTCTGGCCGTAAATTTCGCATTCATGCTACATCACATGCCAGATGAGAGTGTCAGCACTCAAAATCATAGGGATAGGCTTTTAAGGTTGGTTAAGAGCCTATCCCCGAAAGTCGTGACACTTGTTGAGCAAGAATCTAACACAAACACTGCGGCATTCTTTCCACGTTTTCTCGAAACTATGGACTATTACACAGCAATGTTCGAGTCAATAGACGTGACTCTTCCGAGAGAGCATAAAGAGAGGATCAATGTTGAGCAGCATTGTTTGGCAAGAGATTTAGTTAACATCATAGCTTGTGAAGGGGTCGAGAGAGTGGAACGACACGAGCTGCTAGGGAAGTGGAGGTCAAGATTTGCAATGGCTGGTTTCACTCCTTACCCTCTGAGTTCTTTGGTGAATGGTACCATAAAGAAATTGCTTGAAAACTACAGTGATAGATATAGACTTGAAGAGAGAGATGGAGCTCTATATCTTGGTTGGATGAATAGAGATTTGGTTGCTTCTTGTGCTTGGAAATGA
Protein sequence

>MS.gene81215.t1

MQASEQHRSSSMYYQPLQQIEAYCLPQYRNLNHQLYNNDGAQATQFSAPTSSDLYYCTLESSLAAGNFVVYNSPSTVSFSPNDSPMSQQESQSYPSDKYHSPDNTYGSPMSGSCITEDLSSFKHKLRELENVMLGPDSDNLDSYDSAISNGTNFASLEMDSWRQTMVAISSKNLKHILTACAKAIADNDLLMAQWLMDELRQMVSVSGEPIQRLGAYMLEGLVARLSASGSSIYKSLRCKEPESAELLSYMNILYEVCPYFKFGYMSANGAIAEAMKNEARVHIIDFQIAQGSQWISLIQAFAARPGGPPHIRITGIDDPTSAYARGGGLHIVEKRLSKLARHFKVPFEFHAAAISGCDVQLHNLAVRPGEALAVNFAFMLHHMPDESVSTQNHRDRLLRLVKSLSPKVVTLVEQESNTNTAAFFPRFLETMDYYTAMFESIDVTLPREHKERINVEQHCLARDLVNIIACEGVERVERHELLGKWRSRFAMAGFTPYPLSSLVNGTIKKLLENYSDRYRLEERDGALYLGWMNRDLVASCAWK