AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81267

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81267.t1 MTR_5g096790 95.152 165 8 0 1 165 154 318 4.83e-112 322
MS.gene81267.t1 MTR_3g061090 57.059 170 71 1 1 170 86 253 6.72e-58 182
MS.gene81267.t1 MTR_3g061090 57.059 170 71 1 1 170 158 325 1.13e-56 181
MS.gene81267.t1 MTR_5g096700 53.333 165 76 1 1 165 99 262 9.36e-56 177
MS.gene81267.t1 MTR_5g096700 53.333 165 76 1 1 165 158 321 1.21e-54 176
MS.gene81267.t1 MTR_3g088560 42.775 173 96 2 1 172 32 202 3.69e-40 135
MS.gene81267.t1 MTR_1g029850 41.212 165 95 1 1 165 158 320 5.23e-40 138
MS.gene81267.t1 MTR_3g088560 42.775 173 96 2 1 172 84 254 1.86e-39 135
MS.gene81267.t1 MTR_3g088560 42.775 173 96 2 1 172 99 269 3.89e-39 134
MS.gene81267.t1 MTR_3g088560 42.775 173 96 2 1 172 154 324 2.11e-38 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81267.t1 AT1G55760 55.152 165 72 1 1 165 157 319 2.85e-53 172
MS.gene81267.t1 AT1G21780 44.848 165 89 1 1 165 154 316 2.64e-41 141
MS.gene81267.t1 AT1G21780 44.848 165 89 1 1 165 154 316 2.64e-41 141

Find 18 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCAATATCTAGTGGAATT+TGG 0.233852 5.2:-81174039 MS.gene81267:CDS
TTGGTAAAATACATGAAATC+TGG 0.300649 5.2:-81174020 MS.gene81267:CDS
TATGTTTGCACAAGATTCTA+AGG 0.372257 5.2:-81174325 MS.gene81267:CDS
GAATCATAGTTTCGAGCTAT+TGG 0.408610 5.2:-81174202 MS.gene81267:CDS
ATGAATGCTTGACAAACTTC+AGG 0.409567 5.2:+81174264 None:intergenic
ATCTAGCTGTTCTTGCTGCC+CGG 0.416939 5.2:-81174365 MS.gene81267:CDS
ATATCTGAGATGTTAATAAC+AGG 0.455807 5.2:+81174291 None:intergenic
TGGTAAAATACATGAAATCT+GGG 0.484453 5.2:-81174019 MS.gene81267:CDS
TTGGTTGCTGCTGAGAAATA+CGG 0.484832 5.2:-81174183 MS.gene81267:CDS
TATATTCAAACATAGGTGAC+CGG 0.536701 5.2:+81174346 None:intergenic
AGCGCAACTAATCTTCAAGT+AGG 0.555426 5.2:+81174059 None:intergenic
GCAAACATATATTCAAACAT+AGG 0.563128 5.2:+81174339 None:intergenic
GCGCAACTAATCTTCAAGTA+GGG 0.602481 5.2:+81174060 None:intergenic
CGGCGTTAATGATTTGAGAG+AGG 0.605267 5.2:-81174163 MS.gene81267:CDS
TAGTTGCGCTCAATATCTAG+TGG 0.608180 5.2:-81174046 MS.gene81267:CDS
ATTGATAGAAATATGCAGCA+TGG 0.618155 5.2:+81174085 None:intergenic
ATATTCAAACATAGGTGACC+GGG 0.654376 5.2:+81174347 None:intergenic
GCTTGATTGTAGCGTCTGTG+TGG 0.704769 5.2:+81174418 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTTTCTTAGTTAAACT+AGG - chr5.2:81174413-81174432 MS.gene81267:CDS 20.0%
! ACAGAAAAAATATACTTGAG+AGG - chr5.2:81174252-81174271 MS.gene81267:CDS 25.0%
! GCAAACATATATTCAAACAT+AGG + chr5.2:81174026-81174045 None:intergenic 25.0%
! TGGTAAAATACATGAAATCT+GGG - chr5.2:81174343-81174362 MS.gene81267:CDS 25.0%
! TTGGTAAAATACATGAAATC+TGG - chr5.2:81174342-81174361 MS.gene81267:CDS 25.0%
!! ATATCTGAGATGTTAATAAC+AGG + chr5.2:81174074-81174093 None:intergenic 25.0%
ATTGATAGAAATATGCAGCA+TGG + chr5.2:81174280-81174299 None:intergenic 30.0%
TATATTCAAACATAGGTGAC+CGG + chr5.2:81174019-81174038 None:intergenic 30.0%
TATGTTTGCACAAGATTCTA+AGG - chr5.2:81174037-81174056 MS.gene81267:CDS 30.0%
! TTTTCTTGCCTTCCTAAAAT+TGG - chr5.2:81174370-81174389 MS.gene81267:CDS 30.0%
ATATTCAAACATAGGTGACC+GGG + chr5.2:81174018-81174037 None:intergenic 35.0%
ATGAATGCTTGACAAACTTC+AGG + chr5.2:81174101-81174120 None:intergenic 35.0%
GAATCATAGTTTCGAGCTAT+TGG - chr5.2:81174160-81174179 MS.gene81267:CDS 35.0%
GCTCAATATCTAGTGGAATT+TGG - chr5.2:81174323-81174342 MS.gene81267:CDS 35.0%
! CATCATCATCTGCCAATTTT+AGG + chr5.2:81174385-81174404 None:intergenic 35.0%
!! ATCATCTGCCAATTTTAGGA+AGG + chr5.2:81174381-81174400 None:intergenic 35.0%
AGCGCAACTAATCTTCAAGT+AGG + chr5.2:81174306-81174325 None:intergenic 40.0%
GCGCAACTAATCTTCAAGTA+GGG + chr5.2:81174305-81174324 None:intergenic 40.0%
TAGTTGCGCTCAATATCTAG+TGG - chr5.2:81174316-81174335 MS.gene81267:CDS 40.0%
TTGGTTGCTGCTGAGAAATA+CGG - chr5.2:81174179-81174198 MS.gene81267:CDS 40.0%
CGGCGTTAATGATTTGAGAG+AGG - chr5.2:81174199-81174218 MS.gene81267:CDS 45.0%
ATCTAGCTGTTCTTGCTGCC+CGG - chr5.2:81173997-81174016 MS.gene81267:CDS 50.0%
GCTTGATTGTAGCGTCTGTG+TGG + chr5.2:81173947-81173966 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 81173927 81174457 81173927 ID=MS.gene81267
chr5.2 mRNA 81173927 81174457 81173927 ID=MS.gene81267.t1;Parent=MS.gene81267
chr5.2 exon 81173927 81174457 81173927 ID=MS.gene81267.t1.exon1;Parent=MS.gene81267.t1
chr5.2 CDS 81173927 81174457 81173927 ID=cds.MS.gene81267.t1;Parent=MS.gene81267.t1
Gene Sequence

>MS.gene81267

ATGTTAAGAGAAAGTATCCACACAGACGCTACAATCAAGCTCGTATCATCAGATAAAATCATAAGAGTTCATCTAGCTGTTCTTGCTGCCCGGTCACCTATGTTTGAATATATGTTTGCACAAGATTCTAAGGAGAATAACATGCCTGTTATTAACATCTCAGATATGTCACCTGAAGTTTGTCAAGCATTCATAGATTATCTATATGACAACATCGACGATGAAGAACTTGCGAATCATAGTTTCGAGCTATTGGTTGCTGCTGAGAAATACGGCGTTAATGATTTGAGAGAGGAGTGTGAAAAGAGTCTTCAAGAAGATATCAACAGAAAAAATATACTTGAGAGGCTCCATGCTGCATATTTCTATCAATTGCCCTACTTGAAGATTAGTTGCGCTCAATATCTAGTGGAATTTGGTAAAATACATGAAATCTGGGATGATTTTCTTGCCTTCCTAAAATTGGCAGATGATGATGTAATTTGCGAAATTTTCTTAGTTAAACTAGGTTTGAGTACCTTTCAGTTTTGA
Protein sequence

>MS.gene81267.t1

MLRESIHTDATIKLVSSDKIIRVHLAVLAARSPMFEYMFAQDSKENNMPVINISDMSPEVCQAFIDYLYDNIDDEELANHSFELLVAAEKYGVNDLREECEKSLQEDINRKNILERLHAAYFYQLPYLKISCAQYLVEFGKIHEIWDDFLAFLKLADDDVICEIFLVKLGLSTFQF