AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81356

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81356.t1 MTR_5g095070 85.714 280 31 2 1 280 1 271 3.24e-176 487
MS.gene81356.t1 MTR_5g080030 64.198 243 81 4 34 272 44 284 2.64e-96 289
MS.gene81356.t1 MTR_5g080030 63.462 104 33 2 178 280 264 363 1.22e-31 121
MS.gene81356.t1 MTR_1g084150 57.143 147 42 3 148 273 3 149 6.16e-39 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81356.t1 AT1G55650 25.210 238 147 6 34 246 38 269 1.15e-11 64.7

Find 57 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCTTCTTGAGGAATATT+TGG 0.163911 5.2:-80147630 None:intergenic
AGTAAAACAGGTACAAGTTT+AGG 0.215844 5.2:-80146121 None:intergenic
CTTTCTAAGGTCCAGTTAAA+AGG 0.247346 5.2:+80146819 MS.gene81356:intron
AATTTGACTAGAGTAGTTCT+TGG 0.346592 5.2:-80146912 None:intergenic
GTAAAACAGGTACAAGTTTA+GGG 0.365515 5.2:-80146120 None:intergenic
AGTTGTTTCTGCTCTAAAAC+TGG 0.365684 5.2:+80146575 MS.gene81356:CDS
TTCTTGCTTTCCTCTACATA+TGG 0.365771 5.2:-80147864 None:intergenic
AACTACTCTAGTCAAATTAC+AGG 0.369281 5.2:+80146917 MS.gene81356:CDS
TGGTAACCACTTTGGTTCCT+TGG 0.370453 5.2:-80147610 None:intergenic
GATGTCGGACATTGATAAAA+AGG 0.374210 5.2:+80147723 MS.gene81356:CDS
AGGTTGGCCATGAAAAGAAA+TGG 0.384383 5.2:+80146547 MS.gene81356:intron
TTGAATGAGTTTGATATAGA+AGG 0.393011 5.2:-80145795 None:intergenic
TTGAGAAATTGTACTTCTAC+AGG 0.406380 5.2:+80146661 MS.gene81356:CDS
AGAGGAAAGCAAGAAAATAA+AGG 0.412073 5.2:+80147872 MS.gene81356:CDS
TAGAAGGTCTCCAAGTCATT+AGG 0.425705 5.2:-80145779 None:intergenic
TTGTGACCTCCAAGTAAAAC+AGG 0.431064 5.2:-80146133 None:intergenic
ACATTGGTGAGTTGTGTGTT+TGG 0.432104 5.2:-80145725 None:intergenic
GGAATATTTGGTAACCACTT+TGG 0.442677 5.2:-80147618 None:intergenic
CTCACCAATGTTGTCGTGTC+CGG 0.446758 5.2:+80145737 MS.gene81356:CDS
AGTGCACTCAAGATCTTAAG+AGG 0.447998 5.2:+80147934 MS.gene81356:CDS
GGTTGGCCATGAAAAGAAAT+GGG 0.467471 5.2:+80146548 MS.gene81356:intron
TTGGATATCTTTGCCGCCAT+CGG 0.472444 5.2:-80146893 None:intergenic
CCTGCAACCGCCAATGCAAA+AGG 0.477405 5.2:+80146690 MS.gene81356:CDS
TCGACTGAAAGCTGATCATC+AGG 0.477735 5.2:+80147657 MS.gene81356:CDS
ACAAGTTTAGGGATGTTTCT+CGG 0.498193 5.2:-80146109 None:intergenic
AACAAATGGCTATTGATACT+TGG 0.501313 5.2:+80147695 MS.gene81356:CDS
CATACGCGTCCGAAAACAAA+TGG 0.508342 5.2:+80147681 MS.gene81356:CDS
TTCTTACTTAAAGCAGGAGA+TGG 0.509273 5.2:+80147517 MS.gene81356:intron
CTCAATGGAAGACAAGGAAA+TGG 0.517620 5.2:+80145688 None:intergenic
GTCGAGCACATTCCTTCTTG+AGG 0.518278 5.2:-80147639 None:intergenic
TACTTGGAGGTCACAAGAAG+AGG 0.530713 5.2:+80146140 MS.gene81356:CDS
GTATTCTTCTCCTAATGACT+TGG 0.531995 5.2:+80145769 MS.gene81356:CDS
AAGGTCCAGTTAAAAGGAAG+CGG 0.550807 5.2:+80146825 MS.gene81356:intron
CCAATGTTGTCGTGTCCGGC+GGG 0.557640 5.2:+80145741 MS.gene81356:CDS
ACCAATGTTGTCGTGTCCGG+CGG 0.561143 5.2:+80145740 MS.gene81356:CDS
AGGTCCAGTTAAAAGGAAGC+GGG 0.572045 5.2:+80146826 MS.gene81356:intron
GTTTCTGCTCTAAAACTGGA+AGG 0.575106 5.2:+80146579 MS.gene81356:CDS
CCCTAGTTTATCTCAATCAA+CGG 0.576803 5.2:+80146856 MS.gene81356:CDS
TTACCAAATATTCCTCAAGA+AGG 0.579179 5.2:+80147627 MS.gene81356:CDS
CCCGCCGGACACGACAACAT+TGG 0.584078 5.2:-80145741 None:intergenic
CCGTTGATTGAGATAAACTA+GGG 0.589080 5.2:-80146856 None:intergenic
TCCGTTGATTGAGATAAACT+AGG 0.590229 5.2:-80146857 None:intergenic
TTTGCATTGGCGGTTGCAGG+AGG 0.593878 5.2:-80146687 None:intergenic
AGACAACTCAATGGAAGACA+AGG 0.602364 5.2:+80145682 None:intergenic
TTGGAGGTCACAAGAAGAGG+AGG 0.603112 5.2:+80146143 MS.gene81356:CDS
AGAAACAGAGAGGTGCTCCA+AGG 0.610360 5.2:+80147593 MS.gene81356:CDS
TTTCATGTAGCCATATGTAG+AGG 0.614803 5.2:+80147854 MS.gene81356:intron
TGATACTTGGAAGAAGATGT+CGG 0.619673 5.2:+80147708 MS.gene81356:CDS
GGACGATGAAGATTATCCGA+TGG 0.637194 5.2:+80146877 MS.gene81356:CDS
GTGCACTCAAGATCTTAAGA+GGG 0.655456 5.2:+80147935 MS.gene81356:CDS
CAAGGAAATGGTGAAGACGA+CGG 0.673865 5.2:+80145700 MS.gene81356:CDS
CGATGAAGATTATCCGATGG+CGG 0.681671 5.2:+80146880 MS.gene81356:CDS
AGAAGAATACTGTTTCCCGC+CGG 0.682409 5.2:-80145756 None:intergenic
GAAAAGAAGCATAATGTGTG+TGG 0.683498 5.2:+80147960 MS.gene81356:CDS
AAAGAAAAGAAGAAACAGAG+AGG 0.700139 5.2:+80147583 MS.gene81356:CDS
GGTGCTCCAAGGAACCAAAG+TGG 0.716106 5.2:+80147604 MS.gene81356:CDS
GTTGGCCATGAAAAGAAATG+GGG 0.719191 5.2:+80146549 MS.gene81356:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTATTACATATAATTATTT+GGG + chr5.2:80146026-80146045 MS.gene81356:intron 10.0%
!! TGTTATTACATATAATTATT+TGG + chr5.2:80146025-80146044 MS.gene81356:intron 10.0%
!! TTATAAAAAAAAAATTTCCT+CGG + chr5.2:80146475-80146494 MS.gene81356:intron 10.0%
!! ATTAAAAGTTGAAAGATTTA+TGG + chr5.2:80147778-80147797 MS.gene81356:intron 15.0%
!!! GAGGAAATTTTTTTTTTATA+AGG - chr5.2:80146476-80146495 None:intergenic 15.0%
!! ATTATTTGGATGAGTTTAAA+GGG + chr5.2:80147219-80147238 MS.gene81356:intron 20.0%
!! ATTTCAAATAAACCAACTAT+AGG - chr5.2:80147816-80147835 None:intergenic 20.0%
!!! TTCAATTTACAACGTTTTTA+TGG + chr5.2:80146503-80146522 MS.gene81356:intron 20.0%
! ATTTACTAAAGTTCCAAAAC+CGG + chr5.2:80147133-80147152 MS.gene81356:intron 25.0%
! CGAAAATTGAATTCATTGAT+TGG + chr5.2:80146967-80146986 MS.gene81356:intron 25.0%
! GATTATTTGGATGAGTTTAA+AGG + chr5.2:80147218-80147237 MS.gene81356:intron 25.0%
! GTAATCTAAATTAGTCAAGA+GGG + chr5.2:80146425-80146444 MS.gene81356:intron 25.0%
! TAAAATACGAGCTTGAAATA+TGG + chr5.2:80147362-80147381 MS.gene81356:intron 25.0%
! TAATCTAAATTAGTCAAGAG+GGG + chr5.2:80146426-80146445 MS.gene81356:intron 25.0%
! TAGTCAAATTACAGGTAATA+AGG + chr5.2:80146925-80146944 MS.gene81356:intron 25.0%
! TCTTGACTAATTTAGATTAC+AGG - chr5.2:80146425-80146444 None:intergenic 25.0%
! TGCTTAAGATAACCATAAAT+TGG - chr5.2:80146729-80146748 None:intergenic 25.0%
! TGTAATCTAAATTAGTCAAG+AGG + chr5.2:80146424-80146443 MS.gene81356:intron 25.0%
! TTGAAACTGCAAAATAGAAT+TGG - chr5.2:80146086-80146105 None:intergenic 25.0%
! TTGAATGAGTTTGATATAGA+AGG - chr5.2:80145798-80145817 None:intergenic 25.0%
!! TTGGTAACTCAATGATTATT+TGG + chr5.2:80147205-80147224 MS.gene81356:intron 25.0%
!!! TATCAATAGCCATTTGTTTT+CGG - chr5.2:80147693-80147712 None:intergenic 25.0%
!!! TCAAAATCTACTTTGAAATC+TGG - chr5.2:80147324-80147343 None:intergenic 25.0%
!!! TTCTTTTCTTTGTTACTTGA+GGG - chr5.2:80147574-80147593 None:intergenic 25.0%
AAAGAAAAGAAGAAACAGAG+AGG + chr5.2:80147583-80147602 MS.gene81356:CDS 30.0%
AACAAATGGCTATTGATACT+TGG + chr5.2:80147695-80147714 MS.gene81356:CDS 30.0%
AACACAAAAAGTAGCAAAAC+CGG - chr5.2:80147155-80147174 None:intergenic 30.0%
AACTACTCTAGTCAAATTAC+AGG + chr5.2:80146917-80146936 MS.gene81356:CDS 30.0%
AATTTGACTAGAGTAGTTCT+TGG - chr5.2:80146915-80146934 None:intergenic 30.0%
ACCTTAAATACTCCTTTCTA+AGG + chr5.2:80146806-80146825 MS.gene81356:intron 30.0%
AGAGGAAAGCAAGAAAATAA+AGG + chr5.2:80147872-80147891 MS.gene81356:CDS 30.0%
AGTAAAACAGGTACAAGTTT+AGG - chr5.2:80146124-80146143 None:intergenic 30.0%
ATGTAACCAAACAATATACC+TGG - chr5.2:80146175-80146194 None:intergenic 30.0%
ATTCCTTCTTGAGGAATATT+TGG - chr5.2:80147633-80147652 None:intergenic 30.0%
ATTGGATTCTTACTTAAAGC+AGG + chr5.2:80147511-80147530 MS.gene81356:intron 30.0%
ATTTCGTGCATTCATATTCA+AGG + chr5.2:80145911-80145930 MS.gene81356:intron 30.0%
GTAAAACAGGTACAAGTTTA+GGG - chr5.2:80146123-80146142 None:intergenic 30.0%
TTACAGGCACAACATATTTA+TGG - chr5.2:80146409-80146428 None:intergenic 30.0%
TTACCAAATATTCCTCAAGA+AGG + chr5.2:80147627-80147646 MS.gene81356:CDS 30.0%
TTGAGAAATTGTACTTCTAC+AGG + chr5.2:80146661-80146680 MS.gene81356:CDS 30.0%
! AAAAGGTTAACCTTTTGCAT+TGG - chr5.2:80146703-80146722 None:intergenic 30.0%
! AAACTTGTACCTGTTTTACT+TGG + chr5.2:80146124-80146143 MS.gene81356:CDS 30.0%
! ATGCAAAAGGTTAACCTTTT+AGG + chr5.2:80146703-80146722 MS.gene81356:intron 30.0%
! TAACCATAAATTGGCCTAAA+AGG - chr5.2:80146720-80146739 None:intergenic 30.0%
! TAACCTTTTAGGCCAATTTA+TGG + chr5.2:80146714-80146733 MS.gene81356:intron 30.0%
! TGCAAATAGAAGCTACATTT+TGG - chr5.2:80146375-80146394 None:intergenic 30.0%
! TTTTCACCAGGTATATTGTT+TGG + chr5.2:80146166-80146185 MS.gene81356:intron 30.0%
!! ACCTTAGAAAGGAGTATTTA+AGG - chr5.2:80146810-80146829 None:intergenic 30.0%
!! CTTCTTTTCTTTGTTACTTG+AGG - chr5.2:80147575-80147594 None:intergenic 30.0%
!! TTTTGCAATTAGCCTATAGT+TGG + chr5.2:80147801-80147820 MS.gene81356:intron 30.0%
!! TTTTTCAACTTGAGCACATA+AGG - chr5.2:80146616-80146635 None:intergenic 30.0%
!!! TAGAAGCTACATTTTGGTTA+TGG - chr5.2:80146369-80146388 None:intergenic 30.0%
!!! TGTTTCAGTTTGTTGCATTT+TGG + chr5.2:80146758-80146777 MS.gene81356:intron 30.0%
ACAACCCAAATTCAGTTTAG+AGG + chr5.2:80147450-80147469 MS.gene81356:intron 35.0%
ACAAGTTTAGGGATGTTTCT+CGG - chr5.2:80146112-80146131 None:intergenic 35.0%
CACAACTGATCAATTCACTA+TGG + chr5.2:80147103-80147122 MS.gene81356:intron 35.0%
CCCTAGTTTATCTCAATCAA+CGG + chr5.2:80146856-80146875 MS.gene81356:CDS 35.0%
GATGTCGGACATTGATAAAA+AGG + chr5.2:80147723-80147742 MS.gene81356:CDS 35.0%
GCAAAATAGAATTGGAACAG+AGG - chr5.2:80146078-80146097 None:intergenic 35.0%
GCAATGTCGAAAACTATATG+TGG - chr5.2:80146340-80146359 None:intergenic 35.0%
GGTTACATCATATGATGACA+TGG + chr5.2:80146187-80146206 MS.gene81356:intron 35.0%
GTATTCTTCTCCTAATGACT+TGG + chr5.2:80145769-80145788 MS.gene81356:CDS 35.0%
TGATACTTGGAAGAAGATGT+CGG + chr5.2:80147708-80147727 MS.gene81356:CDS 35.0%
TGGATGAGTTTAAAGGGATT+TGG + chr5.2:80147225-80147244 MS.gene81356:intron 35.0%
TTCTCTTGAATGTTTCCTAC+AGG + chr5.2:80146527-80146546 MS.gene81356:intron 35.0%
TTCTTACTTAAAGCAGGAGA+TGG + chr5.2:80147517-80147536 MS.gene81356:intron 35.0%
TTCTTGCTTTCCTCTACATA+TGG - chr5.2:80147867-80147886 None:intergenic 35.0%
TTGTAAATTGAACATCTCCG+AGG - chr5.2:80146495-80146514 None:intergenic 35.0%
TTTCATGTAGCCATATGTAG+AGG + chr5.2:80147854-80147873 MS.gene81356:intron 35.0%
! ATTCAACTTTTAGACTTCGC+TGG + chr5.2:80145812-80145831 MS.gene81356:CDS 35.0%
! CCGTTGATTGAGATAAACTA+GGG - chr5.2:80146859-80146878 None:intergenic 35.0%
! CTGTGGACATGAGTTTATTT+TGG + chr5.2:80147186-80147205 MS.gene81356:intron 35.0%
! GAAAAGAAGCATAATGTGTG+TGG + chr5.2:80147960-80147979 MS.gene81356:CDS 35.0%
! GGAATATTTGGTAACCACTT+TGG - chr5.2:80147621-80147640 None:intergenic 35.0%
! TCCGTTGATTGAGATAAACT+AGG - chr5.2:80146860-80146879 None:intergenic 35.0%
! TTTTAGACTTCGCTGGAAAA+CGG + chr5.2:80145819-80145838 MS.gene81356:CDS 35.0%
!! AAAAGTAGCAAAACCGGTTT+TGG - chr5.2:80147149-80147168 None:intergenic 35.0%
!! AGTTGTTTCTGCTCTAAAAC+TGG + chr5.2:80146575-80146594 MS.gene81356:CDS 35.0%
!! CTTTCTAAGGTCCAGTTAAA+AGG + chr5.2:80146819-80146838 MS.gene81356:intron 35.0%
!! CTTTTAACTGGACCTTAGAA+AGG - chr5.2:80146821-80146840 None:intergenic 35.0%
!! GCTCAAGTTGAAAAACTCTA+TGG + chr5.2:80146621-80146640 MS.gene81356:CDS 35.0%
!! TGTGTGTTTACTGGTTTGTT+TGG + chr5.2:80145877-80145896 MS.gene81356:intron 35.0%
AAGGTCCAGTTAAAAGGAAG+CGG + chr5.2:80146825-80146844 MS.gene81356:intron 40.0%
ACATTGGTGAGTTGTGTGTT+TGG - chr5.2:80145728-80145747 None:intergenic 40.0%
AGCTGCCTCTAAACTGAATT+TGG - chr5.2:80147458-80147477 None:intergenic 40.0%
AGTGCACTCAAGATCTTAAG+AGG + chr5.2:80147934-80147953 MS.gene81356:CDS 40.0%
CCAGTAAACACACATGCAAA+CGG - chr5.2:80145871-80145890 None:intergenic 40.0%
CTTGAATGTTTCCTACAGGT+TGG + chr5.2:80146531-80146550 MS.gene81356:intron 40.0%
GCTGCCTCTAAACTGAATTT+GGG - chr5.2:80147457-80147476 None:intergenic 40.0%
GTGCACTCAAGATCTTAAGA+GGG + chr5.2:80147935-80147954 MS.gene81356:CDS 40.0%
GTGCTTATGCTGCAATACAT+TGG + chr5.2:80147493-80147512 MS.gene81356:intron 40.0%
GTTGGCCATGAAAAGAAATG+GGG + chr5.2:80146549-80146568 MS.gene81356:CDS 40.0%
TACGAGCTTGAAATATGGAC+CGG + chr5.2:80147367-80147386 MS.gene81356:intron 40.0%
TAGAAGGTCTCCAAGTCATT+AGG - chr5.2:80145782-80145801 None:intergenic 40.0%
TGTGTTTATTAGCTGCACTG+TGG + chr5.2:80147169-80147188 MS.gene81356:intron 40.0%
TTGTGACCTCCAAGTAAAAC+AGG - chr5.2:80146136-80146155 None:intergenic 40.0%
! AGGTTAACCTTTTGCATTGG+CGG - chr5.2:80146700-80146719 None:intergenic 40.0%
! AGGTTGGCCATGAAAAGAAA+TGG + chr5.2:80146547-80146566 MS.gene81356:intron 40.0%
! CTTCACCCCATTTCTTTTCA+TGG - chr5.2:80146557-80146576 None:intergenic 40.0%
! CTTGTACCTGTTTTACTTGG+AGG + chr5.2:80146127-80146146 MS.gene81356:CDS 40.0%
! GGTTGGCCATGAAAAGAAAT+GGG + chr5.2:80146548-80146567 MS.gene81356:intron 40.0%
!! GTTTCTGCTCTAAAACTGGA+AGG + chr5.2:80146579-80146598 MS.gene81356:CDS 40.0%
AGAAGAATACTGTTTCCCGC+CGG - chr5.2:80145759-80145778 None:intergenic 45.0%
AGCACATCGTCGTTCAATCA+AGG - chr5.2:80147397-80147416 None:intergenic 45.0%
AGCCTTCAACTTCAACCTAC+AGG + chr5.2:80146261-80146280 MS.gene81356:intron 45.0%
AGGTCCAGTTAAAAGGAAGC+GGG + chr5.2:80146826-80146845 MS.gene81356:intron 45.0%
CAAGGAAATGGTGAAGACGA+CGG + chr5.2:80145700-80145719 MS.gene81356:CDS 45.0%
CATACGCGTCCGAAAACAAA+TGG + chr5.2:80147681-80147700 MS.gene81356:CDS 45.0%
CCGTTTGCATGTGTGTTTAC+TGG + chr5.2:80145868-80145887 MS.gene81356:intron 45.0%
CCTTCAACTTCAACCTACAG+GGG + chr5.2:80146263-80146282 MS.gene81356:intron 45.0%
CGATGAAGATTATCCGATGG+CGG + chr5.2:80146880-80146899 MS.gene81356:CDS 45.0%
GCCTTCAACTTCAACCTACA+GGG + chr5.2:80146262-80146281 MS.gene81356:intron 45.0%
GGACGATGAAGATTATCCGA+TGG + chr5.2:80146877-80146896 MS.gene81356:CDS 45.0%
GGTGGTTCAAACAAGATCTG+CGG + chr5.2:80147297-80147316 MS.gene81356:intron 45.0%
GTGGACACACATTCGAACTT+TGG - chr5.2:80146321-80146340 None:intergenic 45.0%
TACTTGGAGGTCACAAGAAG+AGG + chr5.2:80146140-80146159 MS.gene81356:CDS 45.0%
TCGACTGAAAGCTGATCATC+AGG + chr5.2:80147657-80147676 MS.gene81356:CDS 45.0%
TTGCTAGATTCAACCCCTGT+AGG - chr5.2:80146279-80146298 None:intergenic 45.0%
TTGGATATCTTTGCCGCCAT+CGG - chr5.2:80146896-80146915 None:intergenic 45.0%
! AAGAAGAGGAGGTTTTCACC+AGG + chr5.2:80146154-80146173 MS.gene81356:CDS 45.0%
! TCTTTTCATGGCCAACCTGT+AGG - chr5.2:80146545-80146564 None:intergenic 45.0%
! TGGTAACCACTTTGGTTCCT+TGG - chr5.2:80147613-80147632 None:intergenic 45.0%
CTCACCAATGTTGTCGTGTC+CGG + chr5.2:80145737-80145756 MS.gene81356:CDS 50.0%
GTCGAGCACATTCCTTCTTG+AGG - chr5.2:80147642-80147661 None:intergenic 50.0%
GTCGTTCAATCAAGGTCGTC+CGG - chr5.2:80147389-80147408 None:intergenic 50.0%
TCGCACGCTTTCAACCACAT+CGG + chr5.2:80147273-80147292 MS.gene81356:intron 50.0%
TGTTTGAACCACCACCGATG+TGG - chr5.2:80147290-80147309 None:intergenic 50.0%
TTGGAGGTCACAAGAAGAGG+AGG + chr5.2:80146143-80146162 MS.gene81356:CDS 50.0%
! CCCCTGTAGGTTGAAGTTGA+AGG - chr5.2:80146266-80146285 None:intergenic 50.0%
! GAGTCCCGCTTCCTTTTAAC+TGG - chr5.2:80146833-80146852 None:intergenic 50.0%
!! AGAAACAGAGAGGTGCTCCA+AGG + chr5.2:80147593-80147612 MS.gene81356:CDS 50.0%
ACCAATGTTGTCGTGTCCGG+CGG + chr5.2:80145740-80145759 MS.gene81356:CDS 55.0%
AGCGTGCGATCACAGGATTG+TGG - chr5.2:80147262-80147281 None:intergenic 55.0%
CACGCTTTCAACCACATCGG+TGG + chr5.2:80147276-80147295 MS.gene81356:intron 55.0%
CCTGCAACCGCCAATGCAAA+AGG + chr5.2:80146690-80146709 MS.gene81356:CDS 55.0%
GCTTTCAACCACATCGGTGG+TGG + chr5.2:80147279-80147298 MS.gene81356:intron 55.0%
GGTGCTCCAAGGAACCAAAG+TGG + chr5.2:80147604-80147623 MS.gene81356:CDS 55.0%
! GGTTGAAAGCGTGCGATCAC+AGG - chr5.2:80147269-80147288 None:intergenic 55.0%
! TTTGCATTGGCGGTTGCAGG+AGG - chr5.2:80146690-80146709 None:intergenic 55.0%
!! CCTTTTGCATTGGCGGTTGC+AGG - chr5.2:80146693-80146712 None:intergenic 55.0%
CCAATGTTGTCGTGTCCGGC+GGG + chr5.2:80145741-80145760 MS.gene81356:CDS 60.0%
CCCGCCGGACACGACAACAT+TGG - chr5.2:80145744-80145763 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 80145692 80147992 80145692 ID=MS.gene81356
chr5.2 mRNA 80145692 80147992 80145692 ID=MS.gene81356.t1;Parent=MS.gene81356
chr5.2 exon 80145692 80145840 80145692 ID=MS.gene81356.t1.exon1;Parent=MS.gene81356.t1
chr5.2 CDS 80145692 80145840 80145692 ID=cds.MS.gene81356.t1;Parent=MS.gene81356.t1
chr5.2 exon 80146100 80146175 80146100 ID=MS.gene81356.t1.exon2;Parent=MS.gene81356.t1
chr5.2 CDS 80146100 80146175 80146100 ID=cds.MS.gene81356.t1;Parent=MS.gene81356.t1
chr5.2 exon 80146549 80146711 80146549 ID=MS.gene81356.t1.exon3;Parent=MS.gene81356.t1
chr5.2 CDS 80146549 80146711 80146549 ID=cds.MS.gene81356.t1;Parent=MS.gene81356.t1
chr5.2 exon 80146828 80146938 80146828 ID=MS.gene81356.t1.exon4;Parent=MS.gene81356.t1
chr5.2 CDS 80146828 80146938 80146828 ID=cds.MS.gene81356.t1;Parent=MS.gene81356.t1
chr5.2 exon 80147530 80147744 80147530 ID=MS.gene81356.t1.exon5;Parent=MS.gene81356.t1
chr5.2 CDS 80147530 80147744 80147530 ID=cds.MS.gene81356.t1;Parent=MS.gene81356.t1
chr5.2 exon 80147864 80147992 80147864 ID=MS.gene81356.t1.exon6;Parent=MS.gene81356.t1
chr5.2 CDS 80147864 80147992 80147864 ID=cds.MS.gene81356.t1;Parent=MS.gene81356.t1
Gene Sequence

>MS.gene81356

ATGGAAGACAAGGAAATGGTGAAGACGACGGTTCCAAACACACAACTCACCAATGTTGTCGTGTCCGGCGGGAAACAGTATTCTTCTCCTAATGACTTGGAGACCTTCTATATCAAACTCATTCAACTTTTAGACTTCGCTGGAAAACGGTAAGTTAGTTTCTTCAATCTCATTTTCCGTTTGCATGTGTGTTTACTGGTTTGTTTGGTTTGCTAAAAAATTTCGTGCATTCATATTCAAGGCATTTTGATTTAGAGAGTCTTTTTCTACTCTATTTTCTTTTCATGTGTTTTTCAGTTGAAAAATGATGTGAAATATGAATGAAACATGAAATGTTATTACATATAATTATTTGGGAAAAAATATGATTGATATATAAATGACCTCTGTTCCAATTCTATTTTGCAGTTTCAATGTCCGAGAAACATCCCTAAACTTGTACCTGTTTTACTTGGAGGTCACAAGAAGAGGAGGTTTTCACCAGGTATATTGTTTGGTTACATCATATGATGACATGGTATTGTTCTATATTATTTTTTTAGAAATGCATGTGTATATGTTATATATGTAGCCTTCAACTTCAACCTACAGGGGTTGAATCTAGCAAAAAAGTTAGTCTCAGCAAACCAAAGTTCGAATGTGTGTCCACATATAGTTTTCGACATTGCATAACGCCATAACCAAAATGTAGCTTCTATTTGCATATCAAGTTATCCATAAATATGTTGTGCCTGTAATCTAAATTAGTCAAGAGGGGCTTTTAAGTAGTTTTCATATATATCCTTATAAAAAAAAAATTTCCTCGGAGATGTTCAATTTACAACGTTTTTATGGATTCTCTTGAATGTTTCCTACAGGTTGGCCATGAAAAGAAATGGGGTGAAGTTGTTTCTGCTCTAAAACTGGAAGGAAACAATGCAACCTTATGTGCTCAAGTTGAAAAACTCTATGGTCATCTTCTTTACGAATTTGAGAAATTGTACTTCTACAGGTCTCCTCCTGCAACCGCCAATGCAAAAGGTTAACCTTTTAGGCCAATTTATGGTTATCTTAAGCAGATATTTCTTGTTTCAGTTTGTTGCATTTTGGTATTAAGTTGAGTATGTTCTGTTTAACCTTAAATACTCCTTTCTAAGGTCCAGTTAAAAGGAAGCGGGACTCATGCCCTAGTTTATCTCAATCAACGGACGATGAAGATTATCCGATGGCGGCAAAGATATCCAAGAACTACTCTAGTCAAATTACAGGTAATAAGGATCTAGAATATTAATGATTCGAAAATTGAATTCATTGATTGGATGAAAAAGTATGCAATCAAGTGTTTGTATATGCTAATTAATTAGTAACTAGCTAACTATACTTGATTCAAAATAACTATCATAACTGAATACAACTAACTTTAGTAACTGAACACAACTGATCAATTCACTATGGTAACTGAATTTACTAAAGTTCCAAAACCGGTTTTGCTACTTTTTGTGTTTATTAGCTGCACTGTGGACATGAGTTTATTTTGGTAACTCAATGATTATTTGGATGAGTTTAAAGGGATTTGGATTGTGTGAAGCCACAATCCTGTGATCGCACGCTTTCAACCACATCGGTGGTGGTTCAAACAAGATCTGCGGTCCAGATTTCAAAGTAGATTTTGATATTTTGTTTTATAAATCTAAAATACGAGCTTGAAATATGGACCGGACGACCTTGATTGAACGACGATGTGCTTGAATGAGTACAATCACGAGATTATGACTTCACACAACCCAAATTCAGTTTAGAGGCAGCTCATGTTAATTCAGATGTGCTTATGCTGCAATACATTGGATTCTTACTTAAAGCAGGAGATGGATATCATGAATCTCAAGTGTTTCAACAAGCACCCTCAAGTAACAAAGAAAAGAAGAAACAGAGAGGTGCTCCAAGGAACCAAAGTGGTTACCAAATATTCCTCAAGAAGGAATGTGCTCGACTGAAAGCTGATCATCAGGACATACGCGTCCGAAAACAAATGGCTATTGATACTTGGAAGAAGATGTCGGACATTGATAAAAAGGTACAATATAATGAAATTGACATAATAATATCTATTAAAAGTTGAAAGATTTATGGTTTTGCAATTAGCCTATAGTTGGTTTATTTGAAATTTGCAACTTCTAATTGCTTTTCATGTAGCCATATGTAGAGGAAAGCAAGAAAATAAAGGAAAAAAACAAAGAAGCAATGATAGTTGACAATGAACAGAAGTGCACTCAAGATCTTAAGAGGGATGAAAAGAAGCATAATGTGTGTGGTGACTACTAA
Protein sequence

>MS.gene81356.t1

MEDKEMVKTTVPNTQLTNVVVSGGKQYSSPNDLETFYIKLIQLLDFAGKRFNVRETSLNLYLFYLEVTRRGGFHQVGHEKKWGEVVSALKLEGNNATLCAQVEKLYGHLLYEFEKLYFYRSPPATANAKGPVKRKRDSCPSLSQSTDDEDYPMAAKISKNYSSQITAGDGYHESQVFQQAPSSNKEKKKQRGAPRNQSGYQIFLKKECARLKADHQDIRVRKQMAIDTWKKMSDIDKKPYVEESKKIKEKNKEAMIVDNEQKCTQDLKRDEKKHNVCGDY