Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81453.t1 | KEH37974.1 | 92.5 | 134 | 9 | 1 | 1 | 134 | 93 | 225 | 2.50E-67 | 264.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81453.t1 | A0A072V7G2 | 92.5 | 134 | 9 | 1 | 1 | 134 | 93 | 225 | 1.8e-67 | 264.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene81453.t1 | TF | PLATZ |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81453.t1 | MTR_2g055210 | 92.537 | 134 | 9 | 1 | 1 | 134 | 93 | 225 | 1.37e-88 | 261 |
| MS.gene81453.t1 | MTR_4g094680 | 51.145 | 131 | 57 | 1 | 11 | 134 | 22 | 152 | 6.25e-48 | 154 |
| MS.gene81453.t1 | MTR_2g036170 | 53.600 | 125 | 51 | 2 | 11 | 134 | 37 | 155 | 1.40e-44 | 146 |
| MS.gene81453.t1 | MTR_4g116300 | 51.200 | 125 | 60 | 1 | 11 | 134 | 6 | 130 | 3.74e-42 | 140 |
| MS.gene81453.t1 | MTR_5g007230 | 43.651 | 126 | 69 | 2 | 11 | 134 | 21 | 146 | 5.38e-35 | 120 |
| MS.gene81453.t1 | MTR_7g102790 | 44.697 | 132 | 68 | 3 | 8 | 134 | 4 | 135 | 2.62e-34 | 118 |
| MS.gene81453.t1 | MTR_1g062160 | 43.077 | 130 | 71 | 3 | 8 | 134 | 4 | 133 | 6.21e-34 | 117 |
| MS.gene81453.t1 | MTR_8g066820 | 42.857 | 126 | 70 | 2 | 11 | 134 | 33 | 158 | 6.68e-34 | 118 |
| MS.gene81453.t1 | MTR_4g078170 | 42.857 | 126 | 70 | 2 | 11 | 134 | 15 | 140 | 1.27e-33 | 117 |
| MS.gene81453.t1 | MTR_5g030130 | 43.651 | 126 | 69 | 2 | 11 | 134 | 35 | 160 | 4.66e-33 | 115 |
| MS.gene81453.t1 | MTR_5g030130 | 43.651 | 126 | 69 | 2 | 11 | 134 | 35 | 160 | 9.88e-33 | 115 |
| MS.gene81453.t1 | MTR_2g084495 | 38.849 | 139 | 72 | 6 | 8 | 134 | 2 | 139 | 2.46e-24 | 93.6 |
| MS.gene81453.t1 | MTR_2g084525 | 36.029 | 136 | 76 | 4 | 10 | 134 | 4 | 139 | 5.92e-21 | 85.1 |
| MS.gene81453.t1 | MTR_2g060500 | 36.691 | 139 | 75 | 6 | 8 | 134 | 8 | 145 | 3.78e-20 | 80.9 |
| MS.gene81453.t1 | MTR_2g060540 | 35.971 | 139 | 76 | 6 | 8 | 134 | 8 | 145 | 8.58e-20 | 79.7 |
| MS.gene81453.t1 | MTR_8g105930 | 29.605 | 152 | 84 | 3 | 5 | 134 | 7 | 157 | 4.82e-18 | 77.4 |
| MS.gene81453.t1 | MTR_5g077740 | 31.159 | 138 | 83 | 4 | 4 | 134 | 16 | 148 | 3.66e-17 | 73.9 |
| MS.gene81453.t1 | MTR_2g084510 | 32.353 | 136 | 81 | 4 | 10 | 134 | 3 | 138 | 1.41e-15 | 70.9 |
| MS.gene81453.t1 | MTR_1g032880 | 42.857 | 84 | 42 | 3 | 56 | 134 | 27 | 109 | 1.58e-12 | 61.2 |
| MS.gene81453.t1 | MTR_8g097310 | 32.967 | 91 | 56 | 3 | 49 | 134 | 4 | 94 | 3.62e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81453.t1 | AT3G60670 | 60.000 | 125 | 49 | 1 | 11 | 134 | 7 | 131 | 3.12e-53 | 168 |
| MS.gene81453.t1 | AT1G31040 | 56.452 | 124 | 54 | 0 | 11 | 134 | 19 | 142 | 1.75e-49 | 158 |
| MS.gene81453.t1 | AT2G12646 | 54.400 | 125 | 56 | 1 | 11 | 134 | 3 | 127 | 3.86e-44 | 145 |
| MS.gene81453.t1 | AT2G12646 | 54.400 | 125 | 56 | 1 | 11 | 134 | 6 | 130 | 3.88e-44 | 145 |
| MS.gene81453.t1 | AT2G12646 | 54.400 | 125 | 56 | 1 | 11 | 134 | 6 | 130 | 3.88e-44 | 145 |
| MS.gene81453.t1 | AT1G32700 | 43.846 | 130 | 71 | 2 | 7 | 134 | 8 | 137 | 1.45e-33 | 117 |
| MS.gene81453.t1 | AT4G17900 | 44.094 | 127 | 68 | 3 | 11 | 134 | 20 | 146 | 1.72e-32 | 114 |
| MS.gene81453.t1 | AT4G17900 | 44.094 | 127 | 68 | 3 | 11 | 134 | 34 | 160 | 1.89e-32 | 114 |
| MS.gene81453.t1 | AT1G21000 | 41.270 | 126 | 72 | 2 | 11 | 134 | 13 | 138 | 2.53e-31 | 112 |
| MS.gene81453.t1 | AT1G21000 | 41.270 | 126 | 72 | 2 | 11 | 134 | 16 | 141 | 2.77e-31 | 112 |
| MS.gene81453.t1 | AT2G27930 | 40.441 | 136 | 69 | 3 | 11 | 134 | 4 | 139 | 4.56e-30 | 106 |
| MS.gene81453.t1 | AT2G27930 | 40.441 | 136 | 69 | 3 | 11 | 134 | 4 | 139 | 4.56e-30 | 106 |
| MS.gene81453.t1 | AT1G76590 | 40.769 | 130 | 75 | 2 | 7 | 134 | 14 | 143 | 6.27e-30 | 108 |
| MS.gene81453.t1 | AT2G27930 | 40.441 | 136 | 69 | 3 | 11 | 134 | 4 | 139 | 1.11e-29 | 106 |
| MS.gene81453.t1 | AT2G27930 | 40.441 | 136 | 69 | 3 | 11 | 134 | 4 | 139 | 1.11e-29 | 106 |
| MS.gene81453.t1 | AT1G43000 | 40.769 | 130 | 75 | 2 | 7 | 134 | 5 | 134 | 5.21e-29 | 105 |
| MS.gene81453.t1 | AT1G43000 | 40.769 | 130 | 75 | 2 | 7 | 134 | 12 | 141 | 5.22e-29 | 105 |
| MS.gene81453.t1 | AT2G01818 | 41.353 | 133 | 67 | 3 | 13 | 134 | 13 | 145 | 6.26e-29 | 105 |
| MS.gene81453.t1 | AT5G46710 | 40.625 | 128 | 71 | 4 | 11 | 134 | 28 | 154 | 2.97e-26 | 97.1 |
| MS.gene81453.t1 | AT5G46710 | 40.625 | 128 | 71 | 4 | 11 | 134 | 12 | 138 | 3.47e-26 | 97.8 |
| MS.gene81453.t1 | AT5G46710 | 40.625 | 128 | 71 | 4 | 11 | 134 | 28 | 154 | 7.21e-26 | 97.4 |
| MS.gene81453.t1 | AT1G32700 | 45.918 | 98 | 51 | 2 | 39 | 134 | 1 | 98 | 4.00e-24 | 91.7 |
| MS.gene81453.t1 | AT2G27930 | 41.414 | 99 | 46 | 2 | 46 | 134 | 46 | 142 | 5.32e-17 | 73.6 |
| MS.gene81453.t1 | AT2G27930 | 41.414 | 99 | 46 | 2 | 46 | 134 | 46 | 142 | 5.32e-17 | 73.6 |
| MS.gene81453.t1 | AT2G01818 | 44.737 | 76 | 35 | 2 | 66 | 134 | 6 | 81 | 8.06e-13 | 62.0 |
Find 0 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAACTATGTATAT+TGG | - | 43398:41983-42002 | MS.gene81453:intron | 10.0% |
| !! | ATATATTAAACTATGTATAT+TGG | - | 43398:41953-41972 | MS.gene81453:intron | 10.0% |
| !!! | AATATACATAGTTTTTTTTT+TGG | + | 43398:41984-42003 | MS.gene81453:intergenic | 10.0% |
| !!! | ATTAATTTTTACAAGAATTT+AGG | - | 43398:42010-42029 | MS.gene81453:intron | 10.0% |
| !! | ATGTGTATCATGATATTATA+AGG | - | 43398:41843-41862 | MS.gene81453:intron | 20.0% |
| !! | TAATTCATCATGAAGAAAAA+AGG | - | 43398:41610-41629 | MS.gene81453:CDS | 20.0% |
| ! | AAAACAAGAAAGAGAAAAGA+AGG | + | 43398:41764-41783 | MS.gene81453:intergenic | 25.0% |
| ! | AAACAAGAAAGAGAAAAGAA+GGG | + | 43398:41763-41782 | MS.gene81453:intergenic | 25.0% |
| ! | GTATCATGATATTATAAGGA+TGG | - | 43398:41847-41866 | MS.gene81453:intron | 25.0% |
| !! | CTATTCAAGTAAGTGATTTT+TGG | - | 43398:41900-41919 | MS.gene81453:CDS | 25.0% |
| !!! | TTACCACAATTAATTTGTGT+TGG | - | 43398:42063-42082 | MS.gene81453:CDS | 25.0% |
| ACACCAACACAAATTAATTG+TGG | + | 43398:42069-42088 | MS.gene81452:intergenic | 30.0% | |
| AGAGAGAGCAGAATTTGTAA+GGG | + | 43398:42216-42235 | MS.gene81452:intergenic | 35.0% | |
| CTACAGTTCTACAGAACAAA+CGG | - | 43398:42100-42119 | MS.gene81453:CDS | 35.0% | |
| GAAAGAGAAAAGAAGGGTTA+AGG | + | 43398:41757-41776 | MS.gene81453:intergenic | 35.0% | |
| TTGAGAGAGAAAGAAGAAGA+CGG | + | 43398:41726-41745 | MS.gene81453:intergenic | 35.0% | |
| TTGCTTGAGTAGCATTTCTT+TGG | + | 43398:42160-42179 | MS.gene81453:intergenic | 35.0% | |
| !! | GAGTAGCATTTCTTTGGAAT+TGG | + | 43398:42154-42173 | MS.gene81453:intergenic | 35.0% |
| CAACGTGGAACAACAACATT+TGG | + | 43398:41549-41568 | MS.gene81452:intergenic | 40.0% | |
| GAGAGAGAGCAGAATTTGTA+AGG | + | 43398:42217-42236 | MS.gene81453:intergenic | 40.0% | |
| ! | CAAATGTTGTTGTTCCACGT+TGG | - | 43398:41547-41566 | MS.gene81453:CDS | 40.0% |
| AGCATAACTTGAAGCCAACG+TGG | + | 43398:41564-41583 | MS.gene81452:intergenic | 45.0% | |
| AACCTGCAAGAGTCTGTGAG+AGG | + | 43398:41703-41722 | MS.gene81452:intergenic | 50.0% | |
| GTAAGGGTCTTGCAGACTTC+TGG | + | 43398:42200-42219 | MS.gene81453:intergenic | 50.0% | |
| ! | AAGAGTCTGTGAGAGGCATG+AGG | + | 43398:41696-41715 | MS.gene81453:intergenic | 50.0% |
| GTCTTGCAGACTTCTGGCAC+AGG | + | 43398:42194-42213 | MS.gene81453:intergenic | 55.0% | |
| TGCCTCTCACAGACTCTTGC+AGG | - | 43398:41698-41717 | MS.gene81453:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43398 | gene | 41531 | 42243 | 41531 | ID=MS.gene81453 |
| 43398 | mRNA | 41531 | 42243 | 41531 | ID=MS.gene81453.t1;Parent=MS.gene81453 |
| 43398 | exon | 42055 | 42243 | 42055 | ID=MS.gene81453.t1.exon1;Parent=MS.gene81453.t1 |
| 43398 | CDS | 42055 | 42243 | 42055 | ID=cds.MS.gene81453.t1;Parent=MS.gene81453.t1 |
| 43398 | exon | 41867 | 41941 | 41867 | ID=MS.gene81453.t1.exon2;Parent=MS.gene81453.t1 |
| 43398 | CDS | 41867 | 41941 | 41867 | ID=cds.MS.gene81453.t1;Parent=MS.gene81453.t1 |
| 43398 | exon | 41531 | 41668 | 41531 | ID=MS.gene81453.t1.exon3;Parent=MS.gene81453.t1 |
| 43398 | CDS | 41531 | 41668 | 41531 | ID=cds.MS.gene81453.t1;Parent=MS.gene81453.t1 |
| Gene Sequence |
| Protein sequence |
