Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81548.t1 | XP_022729160.1 | 77.8 | 99 | 19 | 1 | 9 | 107 | 422 | 517 | 7.00E-38 | 166.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81548.t1 | Q39238 | 84.8 | 79 | 11 | 1 | 9 | 86 | 579 | 657 | 1.3e-35 | 150.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81548.t1 | A0A061GUZ6 | 78.8 | 104 | 4 | 1 | 9 | 94 | 588 | 691 | 6.6e-38 | 166.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81548.t1 | MTR_5g086790 | 97.436 | 78 | 2 | 0 | 9 | 86 | 562 | 639 | 1.41e-51 | 173 |
| MS.gene81548.t1 | MTR_5g087050 | 96.154 | 78 | 3 | 0 | 9 | 86 | 494 | 571 | 1.75e-51 | 172 |
| MS.gene81548.t1 | MTR_5g086940 | 94.872 | 78 | 4 | 0 | 9 | 86 | 233 | 310 | 2.87e-50 | 162 |
| MS.gene81548.t1 | MTR_5g086960 | 91.026 | 78 | 7 | 0 | 9 | 86 | 104 | 181 | 1.33e-49 | 157 |
| MS.gene81548.t1 | MTR_5g087240 | 92.308 | 78 | 6 | 0 | 9 | 86 | 506 | 583 | 5.73e-48 | 163 |
| MS.gene81548.t1 | MTR_5g086970 | 76.923 | 78 | 18 | 0 | 9 | 86 | 331 | 408 | 3.12e-39 | 136 |
| MS.gene81548.t1 | MTR_5g087250 | 78.205 | 78 | 17 | 0 | 9 | 86 | 399 | 476 | 4.89e-38 | 134 |
| MS.gene81548.t1 | MTR_3g045010 | 90.909 | 33 | 3 | 0 | 9 | 41 | 150 | 182 | 2.15e-15 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81548.t1 | AT5G20930 | 84.810 | 79 | 11 | 1 | 9 | 86 | 579 | 657 | 1.56e-45 | 157 |
Find 29 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGCCCATTAAAATGTTAA+TGG | 0.189449 | 5.1:+68705461 | MS.gene81548:CDS |
| CGTTCCTGCCCCTATGAAAT+AGG | 0.290825 | 5.1:-68704681 | None:intergenic |
| ATATGCTATGCCTATTTCAT+AGG | 0.292575 | 5.1:+68704671 | MS.gene81548:CDS |
| TCGTGAAGACACAATTATTA+AGG | 0.329793 | 5.1:+68705178 | MS.gene81548:CDS |
| GAAGTATTCTCTCTTGTGTT+TGG | 0.362711 | 5.1:-68705157 | None:intergenic |
| TTGCTGAGCTCAAAGCATTC+AGG | 0.362890 | 5.1:-68704847 | None:intergenic |
| CTCTTCGGCAGACGTCCTTT+CGG | 0.387682 | 5.1:+68705128 | MS.gene81548:CDS |
| TTAGAAACAGTAGGTCTTGC+GGG | 0.397662 | 5.1:-68705218 | None:intergenic |
| CAGGTTGATGTCTGGTCTGC+TGG | 0.447095 | 5.1:+68705086 | MS.gene81548:intron |
| TCCGACTTTATTCTATGTGC+AGG | 0.459765 | 5.1:+68705384 | MS.gene81548:CDS |
| GCAATCATATAACCTAAAAC+AGG | 0.468478 | 5.1:-68705354 | None:intergenic |
| TCATAGGGGCAGGAACGTAT+TGG | 0.474090 | 5.1:+68704687 | MS.gene81548:CDS |
| GTGTAAAACAGGTTGATGTC+TGG | 0.515517 | 5.1:+68705078 | MS.gene81548:intron |
| TACTGTTTCTAATGAGGCAG+AGG | 0.522738 | 5.1:+68705229 | MS.gene81548:CDS |
| TATGCTATGCCTATTTCATA+GGG | 0.528161 | 5.1:+68704672 | MS.gene81548:CDS |
| CACAATTATTAAGGCACGCA+AGG | 0.535508 | 5.1:+68705187 | MS.gene81548:CDS |
| ATGACCTTGGATGATATAAG+AGG | 0.545308 | 5.1:-68704874 | None:intergenic |
| TATGCCTATTTCATAGGGGC+AGG | 0.545706 | 5.1:+68704677 | MS.gene81548:CDS |
| GAATGTGCAAATTCTCTCCT+CGG | 0.546575 | 5.1:+68705432 | MS.gene81548:CDS |
| CACATTCAGATAAATTGTCA+AGG | 0.550459 | 5.1:-68705416 | None:intergenic |
| AAGACCTACTGTTTCTAATG+AGG | 0.577637 | 5.1:+68705223 | MS.gene81548:CDS |
| GACGCCTCTTATATCATCCA+AGG | 0.602777 | 5.1:+68704870 | MS.gene81548:CDS |
| ATTAGAAACAGTAGGTCTTG+CGG | 0.605838 | 5.1:-68705219 | None:intergenic |
| TGTGTTTGGTCATGTCCGAA+AGG | 0.620138 | 5.1:-68705143 | None:intergenic |
| GCCTGCACATAGAATAAAGT+CGG | 0.627537 | 5.1:-68705385 | None:intergenic |
| TAATGGGCATTGTGATACCG+AGG | 0.642641 | 5.1:-68705449 | None:intergenic |
| CTGAGCTCAAAGCATTCAGG+AGG | 0.645161 | 5.1:-68704844 | None:intergenic |
| TCTGCCTCATTAGAAACAGT+AGG | 0.678978 | 5.1:-68705227 | None:intergenic |
| ATGCTATGCCTATTTCATAG+GGG | 0.723782 | 5.1:+68704673 | MS.gene81548:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAATTAATGACCT+TGG | - | chr5.1:68704890-68704909 | None:intergenic | 15.0% |
| !! | TAATATAAATAAATGAACGA+TGG | - | chr5.1:68705306-68705325 | None:intergenic | 15.0% |
| !! | TATATTATTCTATTATTCAG+TGG | + | chr5.1:68705319-68705338 | MS.gene81548:intron | 15.0% |
| !!! | CAAACCATTAACATTTTAAT+GGG | - | chr5.1:68705468-68705487 | None:intergenic | 20.0% |
| !!! | TCAAACCATTAACATTTTAA+TGG | - | chr5.1:68705469-68705488 | None:intergenic | 20.0% |
| ! | AATGCCCATTAAAATGTTAA+TGG | + | chr5.1:68705461-68705480 | MS.gene81548:CDS | 25.0% |
| ! | GCAAATCAAAAATGAAAGAA+AGG | - | chr5.1:68704815-68704834 | None:intergenic | 25.0% |
| !! | AAGTTTAAAACCAACAAGTT+CGG | - | chr5.1:68704759-68704778 | None:intergenic | 25.0% |
| !!! | TAACATAAGTTTTGTGATGT+CGG | + | chr5.1:68704990-68705009 | MS.gene81548:intron | 25.0% |
| !!! | TTCTTTCATTTTTGATTTGC+AGG | + | chr5.1:68704815-68704834 | MS.gene81548:intron | 25.0% |
| !!! | TTGTTTTATCAAATGCTCTT+CGG | + | chr5.1:68705113-68705132 | MS.gene81548:CDS | 25.0% |
| !!! | TTTTTTAAGTCGTGAATACA+TGG | + | chr5.1:68704905-68704924 | MS.gene81548:intron | 25.0% |
| AATGAAAGAAAGGCAAAAAG+AGG | - | chr5.1:68704805-68704824 | None:intergenic | 30.0% | |
| ATATGCTATGCCTATTTCAT+AGG | + | chr5.1:68704671-68704690 | MS.gene81548:CDS | 30.0% | |
| CACATTCAGATAAATTGTCA+AGG | - | chr5.1:68705419-68705438 | None:intergenic | 30.0% | |
| GCAATCATATAACCTAAAAC+AGG | - | chr5.1:68705357-68705376 | None:intergenic | 30.0% | |
| TATGCTATGCCTATTTCATA+GGG | + | chr5.1:68704672-68704691 | MS.gene81548:CDS | 30.0% | |
| TCGTGAAGACACAATTATTA+AGG | + | chr5.1:68705178-68705197 | MS.gene81548:CDS | 30.0% | |
| ! | ATTTCTGACTTGTGTAAAAC+AGG | + | chr5.1:68705067-68705086 | MS.gene81548:intron | 30.0% |
| !! | CTCAATTTGTCAGTCATTTT+TGG | + | chr5.1:68704949-68704968 | MS.gene81548:intron | 30.0% |
| ATGACCTTGGATGATATAAG+AGG | - | chr5.1:68704877-68704896 | None:intergenic | 35.0% | |
| ATGCTATGCCTATTTCATAG+GGG | + | chr5.1:68704673-68704692 | MS.gene81548:CDS | 35.0% | |
| ATTAGAAACAGTAGGTCTTG+CGG | - | chr5.1:68705222-68705241 | None:intergenic | 35.0% | |
| CGGGTAGTACTTAACATAAA+AGG | - | chr5.1:68705265-68705284 | None:intergenic | 35.0% | |
| GTGATGTAGATCATTGTTGT+AGG | + | chr5.1:68704713-68704732 | MS.gene81548:intron | 35.0% | |
| TTGTGCAAACAACTAAGTGT+TGG | + | chr5.1:68705015-68705034 | MS.gene81548:intron | 35.0% | |
| ! | ACACAATCTTCACCTGTTTT+AGG | + | chr5.1:68705342-68705361 | MS.gene81548:CDS | 35.0% |
| ! | GAAGTATTCTCTCTTGTGTT+TGG | - | chr5.1:68705160-68705179 | None:intergenic | 35.0% |
| !! | AAGACCTACTGTTTCTAATG+AGG | + | chr5.1:68705223-68705242 | MS.gene81548:CDS | 35.0% |
| !!! | TGTCAGTCATTTTTGGTCAA+TGG | + | chr5.1:68704956-68704975 | MS.gene81548:intron | 35.0% |
| ACCCGTAGCTAATTGTTCTT+TGG | + | chr5.1:68705280-68705299 | MS.gene81548:intron | 40.0% | |
| CACAATTATTAAGGCACGCA+AGG | + | chr5.1:68705187-68705206 | MS.gene81548:CDS | 40.0% | |
| GAATGTGCAAATTCTCTCCT+CGG | + | chr5.1:68705432-68705451 | MS.gene81548:CDS | 40.0% | |
| GCCAAAGAACAATTAGCTAC+GGG | - | chr5.1:68705284-68705303 | None:intergenic | 40.0% | |
| GCCTGCACATAGAATAAAGT+CGG | - | chr5.1:68705388-68705407 | None:intergenic | 40.0% | |
| GGCCAAAGAACAATTAGCTA+CGG | - | chr5.1:68705285-68705304 | None:intergenic | 40.0% | |
| TCCGACTTTATTCTATGTGC+AGG | + | chr5.1:68705384-68705403 | MS.gene81548:CDS | 40.0% | |
| TCTGCCTCATTAGAAACAGT+AGG | - | chr5.1:68705230-68705249 | None:intergenic | 40.0% | |
| TTAGAAACAGTAGGTCTTGC+GGG | - | chr5.1:68705221-68705240 | None:intergenic | 40.0% | |
| ! | TTGAAGTGTTCCGAACTTGT+TGG | + | chr5.1:68704746-68704765 | MS.gene81548:intron | 40.0% |
| !! | GTGTAAAACAGGTTGATGTC+TGG | + | chr5.1:68705078-68705097 | MS.gene81548:intron | 40.0% |
| !! | TACTGTTTCTAATGAGGCAG+AGG | + | chr5.1:68705229-68705248 | MS.gene81548:CDS | 40.0% |
| GACGCCTCTTATATCATCCA+AGG | + | chr5.1:68704870-68704889 | MS.gene81548:CDS | 45.0% | |
| GTTTCTAATGAGGCAGAGGT+AGG | + | chr5.1:68705233-68705252 | MS.gene81548:intron | 45.0% | |
| TATGCCTATTTCATAGGGGC+AGG | + | chr5.1:68704677-68704696 | MS.gene81548:CDS | 45.0% | |
| TGTGTTTGGTCATGTCCGAA+AGG | - | chr5.1:68705146-68705165 | None:intergenic | 45.0% | |
| ! | TAATGGGCATTGTGATACCG+AGG | - | chr5.1:68705452-68705471 | None:intergenic | 45.0% |
| ! | TTGCTGAGCTCAAAGCATTC+AGG | - | chr5.1:68704850-68704869 | None:intergenic | 45.0% |
| CGTTCCTGCCCCTATGAAAT+AGG | - | chr5.1:68704684-68704703 | None:intergenic | 50.0% | |
| TCATAGGGGCAGGAACGTAT+TGG | + | chr5.1:68704687-68704706 | MS.gene81548:CDS | 50.0% | |
| ! | CTGAGCTCAAAGCATTCAGG+AGG | - | chr5.1:68704847-68704866 | None:intergenic | 50.0% |
| CTCTTCGGCAGACGTCCTTT+CGG | + | chr5.1:68705128-68705147 | MS.gene81548:CDS | 55.0% | |
| !! | CAGGTTGATGTCTGGTCTGC+TGG | + | chr5.1:68705086-68705105 | MS.gene81548:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 68704668 | 68705488 | 68704668 | ID=MS.gene81548 |
| chr5.1 | mRNA | 68704668 | 68705488 | 68704668 | ID=MS.gene81548.t1;Parent=MS.gene81548 |
| chr5.1 | exon | 68704668 | 68704708 | 68704668 | ID=MS.gene81548.t1.exon1;Parent=MS.gene81548.t1 |
| chr5.1 | CDS | 68704668 | 68704708 | 68704668 | ID=cds.MS.gene81548.t1;Parent=MS.gene81548.t1 |
| chr5.1 | exon | 68704837 | 68704891 | 68704837 | ID=MS.gene81548.t1.exon2;Parent=MS.gene81548.t1 |
| chr5.1 | CDS | 68704837 | 68704891 | 68704837 | ID=cds.MS.gene81548.t1;Parent=MS.gene81548.t1 |
| chr5.1 | exon | 68705089 | 68705250 | 68705089 | ID=MS.gene81548.t1.exon3;Parent=MS.gene81548.t1 |
| chr5.1 | CDS | 68705089 | 68705250 | 68705089 | ID=cds.MS.gene81548.t1;Parent=MS.gene81548.t1 |
| chr5.1 | exon | 68705339 | 68705488 | 68705339 | ID=MS.gene81548.t1.exon4;Parent=MS.gene81548.t1 |
| chr5.1 | CDS | 68705339 | 68705488 | 68705339 | ID=cds.MS.gene81548.t1;Parent=MS.gene81548.t1 |
| Gene Sequence |
| Protein sequence |
