Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81553.t1 | EOY33208.1 | 93.7 | 79 | 5 | 0 | 9 | 87 | 588 | 666 | 1.10E-36 | 162.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81553.t1 | Q39238 | 83.5 | 79 | 12 | 1 | 9 | 86 | 579 | 657 | 1.5e-34 | 146.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81553.t1 | A0A061GUZ6 | 93.7 | 79 | 5 | 0 | 9 | 87 | 588 | 666 | 8.1e-37 | 162.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81553.t1 | MTR_5g086940 | 93.750 | 80 | 5 | 0 | 9 | 88 | 233 | 312 | 5.39e-51 | 163 |
| MS.gene81553.t1 | MTR_5g086790 | 96.154 | 78 | 3 | 0 | 9 | 86 | 562 | 639 | 2.35e-50 | 169 |
| MS.gene81553.t1 | MTR_5g087050 | 94.872 | 78 | 4 | 0 | 9 | 86 | 494 | 571 | 3.05e-50 | 167 |
| MS.gene81553.t1 | MTR_5g086960 | 89.873 | 79 | 8 | 0 | 9 | 87 | 104 | 182 | 2.84e-49 | 155 |
| MS.gene81553.t1 | MTR_5g087240 | 91.026 | 78 | 7 | 0 | 9 | 86 | 506 | 583 | 9.28e-47 | 159 |
| MS.gene81553.t1 | MTR_5g086970 | 77.215 | 79 | 18 | 0 | 9 | 87 | 331 | 409 | 4.77e-39 | 135 |
| MS.gene81553.t1 | MTR_5g087250 | 78.205 | 78 | 17 | 0 | 9 | 86 | 399 | 476 | 1.19e-37 | 132 |
| MS.gene81553.t1 | MTR_3g045010 | 85.714 | 35 | 5 | 0 | 9 | 43 | 150 | 184 | 6.56e-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81553.t1 | AT5G20930 | 83.544 | 79 | 12 | 1 | 9 | 86 | 579 | 657 | 2.01e-44 | 153 |
Find 22 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGGCCATTAAAATGTTAA+TGG | 0.216350 | 5.1:+68587330 | MS.gene81553:CDS |
| ATATGCTATGCCTATTTCAT+AGG | 0.292575 | 5.1:+68586542 | MS.gene81553:CDS |
| ATTACCTACCTTTGCCTCAT+TGG | 0.305618 | 5.1:-68587105 | None:intergenic |
| CGTTCCAGCCCCTATGAAAT+AGG | 0.308188 | 5.1:-68586552 | None:intergenic |
| TCGTGAAGACACAATTATTA+AGG | 0.329793 | 5.1:+68587046 | MS.gene81553:CDS |
| GAAGTATTCTCTCTTGTGTT+TGG | 0.362711 | 5.1:-68587025 | None:intergenic |
| TTGCTGAGCTCAAAGCATTC+AGG | 0.362890 | 5.1:-68586718 | None:intergenic |
| CTCATCGGAAGACGTCCTTT+CGG | 0.401676 | 5.1:+68586996 | MS.gene81553:CDS |
| CAGGTTGATGTCTGGTCTGC+TGG | 0.447095 | 5.1:+68586954 | MS.gene81553:intron |
| TCATAGGGGCTGGAACGTAT+TGG | 0.470439 | 5.1:+68586558 | MS.gene81553:CDS |
| GTGGAAAACAGGTTGATGTC+TGG | 0.502498 | 5.1:+68586946 | MS.gene81553:intron |
| CACAATTATTAAGGCACGCA+TGG | 0.525171 | 5.1:+68587055 | MS.gene81553:CDS |
| TATGCTATGCCTATTTCATA+GGG | 0.525989 | 5.1:+68586543 | MS.gene81553:CDS |
| ATGACCTTGGATGATATAAG+AGG | 0.545308 | 5.1:-68586745 | None:intergenic |
| TATGCCTATTTCATAGGGGC+TGG | 0.545554 | 5.1:+68586548 | MS.gene81553:CDS |
| GAATGTGCAAATTCTCTCCT+CGG | 0.546575 | 5.1:+68587301 | MS.gene81553:CDS |
| GACGCCTCTTATATCATCCA+AGG | 0.602777 | 5.1:+68586741 | MS.gene81553:CDS |
| TGTGTTTGGTCATGTCCGAA+AGG | 0.620138 | 5.1:-68587011 | None:intergenic |
| TAATGGCCATTGTGATACCG+AGG | 0.642641 | 5.1:-68587318 | None:intergenic |
| CTGAGCTCAAAGCATTCAGG+AGG | 0.645161 | 5.1:-68586715 | None:intergenic |
| TTCTCTCCTCGGTATCACAA+TGG | 0.664965 | 5.1:+68587312 | MS.gene81553:CDS |
| ATGCTATGCCTATTTCATAG+GGG | 0.698120 | 5.1:+68586544 | MS.gene81553:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAATTAATGACCT+TGG | - | chr5.1:68586761-68586780 | None:intergenic | 15.0% |
| !! | TATATTATTCTATTATTCAG+TGG | + | chr5.1:68587188-68587207 | MS.gene81553:intron | 15.0% |
| !!! | TCAAACCATTAACATTTTAA+TGG | - | chr5.1:68587338-68587357 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTTAAGTCGTGAA+TGG | + | chr5.1:68586776-68586795 | MS.gene81553:intron | 20.0% |
| ! | AATGGCCATTAAAATGTTAA+TGG | + | chr5.1:68587330-68587349 | MS.gene81553:CDS | 25.0% |
| ! | CTGGTAATACTTATCATAAA+AGG | - | chr5.1:68587136-68587155 | None:intergenic | 25.0% |
| ! | GCAAATCAAAAATGAAAGAA+AGG | - | chr5.1:68586686-68586705 | None:intergenic | 25.0% |
| ! | GCAATAATAAAACCTAAAAC+AGG | - | chr5.1:68587226-68587245 | None:intergenic | 25.0% |
| !! | AAGTTTAAAACCAACAAGTT+TGG | - | chr5.1:68586630-68586649 | None:intergenic | 25.0% |
| !! | CACATTCAGATAAATTTTCA+AGG | - | chr5.1:68587288-68587307 | None:intergenic | 25.0% |
| !!! | TTCTTTCATTTTTGATTTGC+AGG | + | chr5.1:68586686-68586705 | MS.gene81553:intron | 25.0% |
| !!! | TTGTTTTATCAAATGCTCAT+CGG | + | chr5.1:68586981-68587000 | MS.gene81553:CDS | 25.0% |
| ATATGCTATGCCTATTTCAT+AGG | + | chr5.1:68586542-68586561 | MS.gene81553:CDS | 30.0% | |
| TAACATAAGTTTCGTGATGT+CGG | + | chr5.1:68586863-68586882 | MS.gene81553:intron | 30.0% | |
| TATGCTATGCCTATTTCATA+GGG | + | chr5.1:68586543-68586562 | MS.gene81553:CDS | 30.0% | |
| TCGTGAAGACACAATTATTA+AGG | + | chr5.1:68587046-68587065 | MS.gene81553:CDS | 30.0% | |
| ! | TCTCAATTTGTCAGTCATTT+TGG | + | chr5.1:68586822-68586841 | MS.gene81553:intron | 30.0% |
| !!! | TTTTTTAAGTCGTGAATGGA+TGG | + | chr5.1:68586780-68586799 | MS.gene81553:intron | 30.0% |
| ATGACCTTGGATGATATAAG+AGG | - | chr5.1:68586748-68586767 | None:intergenic | 35.0% | |
| ATGCTATGCCTATTTCATAG+GGG | + | chr5.1:68586544-68586563 | MS.gene81553:CDS | 35.0% | |
| ATTGGAAAAAGTAGGTCTTG+AGG | - | chr5.1:68587090-68587109 | None:intergenic | 35.0% | |
| ATTGTGCAAACAACTAGTGT+TGG | + | chr5.1:68586887-68586906 | MS.gene81553:intron | 35.0% | |
| GTGATGAAGATCATTGTTGT+AGG | + | chr5.1:68586584-68586603 | MS.gene81553:intron | 35.0% | |
| TCCTGCACATAGAATAAAGT+CGG | - | chr5.1:68587257-68587276 | None:intergenic | 35.0% | |
| TGGACAGAACAATTAGATAC+TGG | - | chr5.1:68587155-68587174 | None:intergenic | 35.0% | |
| TTGGAAAAAGTAGGTCTTGA+GGG | - | chr5.1:68587089-68587108 | None:intergenic | 35.0% | |
| TTTGCCTCATTGGAAAAAGT+AGG | - | chr5.1:68587098-68587117 | None:intergenic | 35.0% | |
| ! | ACACAATCTTTGCCTGTTTT+AGG | + | chr5.1:68587211-68587230 | MS.gene81553:intron | 35.0% |
| ! | ATTTCTGACTTGTGGAAAAC+AGG | + | chr5.1:68586935-68586954 | MS.gene81553:intron | 35.0% |
| ! | CACATTACATTTCTGACTTG+TGG | + | chr5.1:68586927-68586946 | MS.gene81553:intron | 35.0% |
| ! | GAAGTATTCTCTCTTGTGTT+TGG | - | chr5.1:68587028-68587047 | None:intergenic | 35.0% |
| ! | TTGAAGTGTTCCAAACTTGT+TGG | + | chr5.1:68586617-68586636 | MS.gene81553:intron | 35.0% |
| !! | TTGTCAGTCATTTTGGTCAA+TGG | + | chr5.1:68586829-68586848 | MS.gene81553:intron | 35.0% |
| !!! | AAGACCTACTTTTTCCAATG+AGG | + | chr5.1:68587091-68587110 | MS.gene81553:CDS | 35.0% |
| !!! | TACTTTTTCCAATGAGGCAA+AGG | + | chr5.1:68587097-68587116 | MS.gene81553:CDS | 35.0% |
| ATTACCTACCTTTGCCTCAT+TGG | - | chr5.1:68587108-68587127 | None:intergenic | 40.0% | |
| CACAATTATTAAGGCACGCA+TGG | + | chr5.1:68587055-68587074 | MS.gene81553:CDS | 40.0% | |
| GAATGTGCAAATTCTCTCCT+CGG | + | chr5.1:68587301-68587320 | MS.gene81553:CDS | 40.0% | |
| TCCGACTTTATTCTATGTGC+AGG | + | chr5.1:68587253-68587272 | MS.gene81553:intron | 40.0% | |
| ! | TTTTCCAATGAGGCAAAGGT+AGG | + | chr5.1:68587101-68587120 | MS.gene81553:CDS | 40.0% |
| GACGCCTCTTATATCATCCA+AGG | + | chr5.1:68586741-68586760 | MS.gene81553:CDS | 45.0% | |
| TAATGGCCATTGTGATACCG+AGG | - | chr5.1:68587321-68587340 | None:intergenic | 45.0% | |
| TATGCCTATTTCATAGGGGC+TGG | + | chr5.1:68586548-68586567 | MS.gene81553:CDS | 45.0% | |
| TGTGTTTGGTCATGTCCGAA+AGG | - | chr5.1:68587014-68587033 | None:intergenic | 45.0% | |
| TTCTCTCCTCGGTATCACAA+TGG | + | chr5.1:68587312-68587331 | MS.gene81553:CDS | 45.0% | |
| ! | TTGCTGAGCTCAAAGCATTC+AGG | - | chr5.1:68586721-68586740 | None:intergenic | 45.0% |
| !! | GTGGAAAACAGGTTGATGTC+TGG | + | chr5.1:68586946-68586965 | MS.gene81553:intron | 45.0% |
| !! | TAATATAAATAAATGAAATA+TGG | - | chr5.1:68587175-68587194 | None:intergenic | 5.0% |
| CGTTCCAGCCCCTATGAAAT+AGG | - | chr5.1:68586555-68586574 | None:intergenic | 50.0% | |
| CTCATCGGAAGACGTCCTTT+CGG | + | chr5.1:68586996-68587015 | MS.gene81553:CDS | 50.0% | |
| TCATAGGGGCTGGAACGTAT+TGG | + | chr5.1:68586558-68586577 | MS.gene81553:CDS | 50.0% | |
| ! | CTGAGCTCAAAGCATTCAGG+AGG | - | chr5.1:68586718-68586737 | None:intergenic | 50.0% |
| !! | CAGGTTGATGTCTGGTCTGC+TGG | + | chr5.1:68586954-68586973 | MS.gene81553:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 68586539 | 68587357 | 68586539 | ID=MS.gene81553 |
| chr5.1 | mRNA | 68586539 | 68587357 | 68586539 | ID=MS.gene81553.t1;Parent=MS.gene81553 |
| chr5.1 | exon | 68586539 | 68586579 | 68586539 | ID=MS.gene81553.t1.exon1;Parent=MS.gene81553.t1 |
| chr5.1 | CDS | 68586539 | 68586579 | 68586539 | ID=cds.MS.gene81553.t1;Parent=MS.gene81553.t1 |
| chr5.1 | exon | 68586708 | 68586762 | 68586708 | ID=MS.gene81553.t1.exon2;Parent=MS.gene81553.t1 |
| chr5.1 | CDS | 68586708 | 68586762 | 68586708 | ID=cds.MS.gene81553.t1;Parent=MS.gene81553.t1 |
| chr5.1 | exon | 68586957 | 68587122 | 68586957 | ID=MS.gene81553.t1.exon3;Parent=MS.gene81553.t1 |
| chr5.1 | CDS | 68586957 | 68587122 | 68586957 | ID=cds.MS.gene81553.t1;Parent=MS.gene81553.t1 |
| chr5.1 | exon | 68587275 | 68587357 | 68587275 | ID=MS.gene81553.t1.exon4;Parent=MS.gene81553.t1 |
| chr5.1 | CDS | 68587275 | 68587357 | 68587275 | ID=cds.MS.gene81553.t1;Parent=MS.gene81553.t1 |
| Gene Sequence |
| Protein sequence |
