Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81936.t1 | RHN72439.1 | 74.4 | 211 | 54 | 0 | 165 | 375 | 104 | 314 | 8.40E-89 | 337.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81936.t1 | Q9SZL8 | 34.8 | 210 | 134 | 3 | 166 | 374 | 104 | 311 | 4.6e-27 | 123.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81936.t1 | A0A396J342 | 74.4 | 211 | 54 | 0 | 165 | 375 | 104 | 314 | 6.0e-89 | 337.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene81936.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81936.t1 | MTR_7g446480 | 60.963 | 374 | 73 | 5 | 1 | 374 | 1 | 301 | 5.05e-145 | 414 |
| MS.gene81936.t1 | MTR_7g446520 | 58.974 | 351 | 48 | 3 | 61 | 373 | 15 | 307 | 1.57e-129 | 375 |
| MS.gene81936.t1 | MTR_7g028855 | 57.233 | 318 | 42 | 6 | 1 | 279 | 1 | 263 | 1.88e-100 | 305 |
| MS.gene81936.t1 | MTR_7g446510 | 84.568 | 162 | 25 | 0 | 213 | 374 | 155 | 316 | 5.40e-96 | 290 |
| MS.gene81936.t1 | MTR_5g076070 | 30.303 | 264 | 162 | 4 | 124 | 367 | 21 | 282 | 3.87e-37 | 135 |
| MS.gene81936.t1 | MTR_1g070355 | 36.493 | 211 | 132 | 1 | 165 | 375 | 103 | 311 | 7.31e-37 | 140 |
| MS.gene81936.t1 | MTR_6g051425 | 31.313 | 198 | 134 | 2 | 178 | 374 | 61 | 257 | 1.38e-27 | 111 |
| MS.gene81936.t1 | MTR_6g022080 | 26.357 | 129 | 95 | 0 | 170 | 298 | 119 | 247 | 7.52e-15 | 73.9 |
| MS.gene81936.t1 | MTR_4g035820 | 26.821 | 302 | 195 | 9 | 94 | 374 | 19 | 315 | 3.37e-13 | 71.2 |
| MS.gene81936.t1 | MTR_0402s0040 | 33.793 | 145 | 85 | 3 | 172 | 312 | 63 | 200 | 8.12e-12 | 67.0 |
| MS.gene81936.t1 | MTR_1g008290 | 30.469 | 128 | 84 | 2 | 204 | 331 | 18 | 140 | 1.22e-11 | 62.4 |
| MS.gene81936.t1 | MTR_0558s0020 | 25.359 | 209 | 136 | 6 | 180 | 372 | 85 | 289 | 2.20e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81936.t1 | AT4G38180 | 35.149 | 202 | 128 | 3 | 174 | 374 | 112 | 311 | 4.51e-29 | 119 |
| MS.gene81936.t1 | AT4G15090 | 33.533 | 167 | 108 | 3 | 206 | 371 | 120 | 284 | 3.66e-19 | 89.7 |
| MS.gene81936.t1 | AT4G15090 | 33.533 | 167 | 108 | 3 | 206 | 371 | 120 | 284 | 3.66e-19 | 89.7 |
| MS.gene81936.t1 | AT4G15090 | 33.533 | 167 | 108 | 3 | 206 | 371 | 120 | 284 | 3.66e-19 | 89.7 |
| MS.gene81936.t1 | AT4G15090 | 33.533 | 167 | 108 | 3 | 206 | 371 | 120 | 284 | 3.66e-19 | 89.7 |
| MS.gene81936.t1 | AT4G15090 | 33.533 | 167 | 108 | 3 | 206 | 371 | 120 | 284 | 3.66e-19 | 89.7 |
| MS.gene81936.t1 | AT1G10240 | 29.949 | 197 | 133 | 4 | 176 | 369 | 90 | 284 | 1.69e-13 | 72.4 |
| MS.gene81936.t1 | AT1G10240 | 29.949 | 197 | 133 | 4 | 176 | 369 | 90 | 284 | 1.69e-13 | 72.4 |
| MS.gene81936.t1 | AT3G22170 | 30.409 | 171 | 95 | 3 | 204 | 374 | 150 | 296 | 1.47e-12 | 69.7 |
| MS.gene81936.t1 | AT3G22170 | 30.409 | 171 | 95 | 3 | 204 | 374 | 150 | 296 | 1.47e-12 | 69.7 |
Find 0 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TACGATTTTTATTATAAGTA+CGG | + | 43366:128341-128360 | MS.gene81936:intron | 15.0% |
| !! | AATAAGAACTTGTCATATAA+TGG | + | 43366:128703-128722 | MS.gene81936:CDS | 20.0% |
| !! | ACTTAACATTCATATAGTTT+AGG | - | 43366:128847-128866 | None:intergenic | 20.0% |
| !! | ATAAGAACTTGTCATATAAT+GGG | + | 43366:128704-128723 | MS.gene81936:CDS | 20.0% |
| !! | CTTAACATTCATATAGTTTA+GGG | - | 43366:128846-128865 | None:intergenic | 20.0% |
| !!! | AATTTTACGAAAAATACTCA+AGG | + | 43366:127973-127992 | MS.gene81936:CDS | 20.0% |
| !!! | ATTTTACGAAAAATACTCAA+GGG | + | 43366:127974-127993 | MS.gene81936:CDS | 20.0% |
| ! | AAACAACAACAATGACTTTA+CGG | + | 43366:127789-127808 | MS.gene81936:CDS | 25.0% |
| ! | ATAAATTCATTAACTGCTGA+AGG | + | 43366:128281-128300 | MS.gene81936:intron | 25.0% |
| !!! | ACGTTTGAAATGTCTATTTT+GGG | + | 43366:128916-128935 | MS.gene81936:CDS | 25.0% |
| !!! | AGATTAGATTATTTCTGCTT+TGG | + | 43366:128953-128972 | MS.gene81936:CDS | 25.0% |
| AAAAATGCGTGCTCTTATTA+AGG | + | 43366:128802-128821 | MS.gene81936:CDS | 30.0% | |
| AAACATATCCGATCATTGTT+GGG | + | 43366:127866-127885 | MS.gene81936:CDS | 30.0% | |
| AGCATTTATCAAGGTTAGAT+AGG | + | 43366:128471-128490 | MS.gene81936:CDS | 30.0% | |
| GAATATTCACAAAAGGAATG+TGG | - | 43366:128881-128900 | None:intergenic | 30.0% | |
| GATTATAATGCTGGTGATAA+AGG | + | 43366:128063-128082 | MS.gene81936:CDS | 30.0% | |
| TGTTTATCGGAAAATATCTG+AGG | + | 43366:128643-128662 | MS.gene81936:CDS | 30.0% | |
| ! | AACAATGACTTTACGGATTT+TGG | + | 43366:127796-127815 | MS.gene81936:CDS | 30.0% |
| ! | ACAACATATCTACCCTTTTT+GGG | - | 43366:128560-128579 | None:intergenic | 30.0% |
| ! | CAGATATTTTCCGATAAACA+GGG | - | 43366:128643-128662 | None:intergenic | 30.0% |
| ! | TCAGATATTTTCCGATAAAC+AGG | - | 43366:128644-128663 | None:intergenic | 30.0% |
| ! | TTGTCATATAATGGGATACA+TGG | + | 43366:128712-128731 | MS.gene81936:CDS | 30.0% |
| !! | GACGTTTGAAATGTCTATTT+TGG | + | 43366:128915-128934 | MS.gene81936:CDS | 30.0% |
| !!! | AGTGTAAAGTTTTTGCTGTT+AGG | + | 43366:128366-128385 | MS.gene81936:intron | 30.0% |
| !!! | GTTGTGTCTATTTTTGAAGA+GGG | + | 43366:128575-128594 | MS.gene81936:CDS | 30.0% |
| !!! | TGTTGTGTCTATTTTTGAAG+AGG | + | 43366:128574-128593 | MS.gene81936:CDS | 30.0% |
| AGTGAATGTGATGAAAGTCA+AGG | + | 43366:128099-128118 | MS.gene81936:CDS | 35.0% | |
| AGTGGCAGAATATTCACAAA+AGG | - | 43366:128888-128907 | None:intergenic | 35.0% | |
| CATTAACTGCTGAAGGAATA+AGG | + | 43366:128288-128307 | MS.gene81936:intron | 35.0% | |
| CTGTTAGGAAGAGTGATATT+AGG | + | 43366:128381-128400 | MS.gene81936:intron | 35.0% | |
| GAAACATATCCGATCATTGT+TGG | + | 43366:127865-127884 | MS.gene81936:CDS | 35.0% | |
| GATATTAGGCGCAATGTTAA+CGG | + | 43366:128395-128414 | MS.gene81936:CDS | 35.0% | |
| GTAGATAAAGCACAGATTGA+TGG | + | 43366:128665-128684 | MS.gene81936:CDS | 35.0% | |
| GTTCATTACAGACCCAAAAA+GGG | + | 43366:128545-128564 | MS.gene81936:CDS | 35.0% | |
| TGCATTTACACCCTGTTTAT+CGG | + | 43366:128630-128649 | MS.gene81936:CDS | 35.0% | |
| TGCTGAAGGAATAAGGAAAA+TGG | + | 43366:128295-128314 | MS.gene81936:intron | 35.0% | |
| TGTAAATGCACAACATTGGT+TGG | - | 43366:128620-128639 | None:intergenic | 35.0% | |
| TGTTCATTACAGACCCAAAA+AGG | + | 43366:128544-128563 | MS.gene81936:CDS | 35.0% | |
| TGTTCTGGTGATTATAATGC+TGG | + | 43366:128054-128073 | MS.gene81936:CDS | 35.0% | |
| TTAACGGTGAGATTGTAATG+AGG | + | 43366:128411-128430 | MS.gene81936:CDS | 35.0% | |
| ! | ATGTAGTTGTTGGTGAGAAA+TGG | + | 43366:128253-128272 | MS.gene81936:CDS | 35.0% |
| ! | CACAACATATCTACCCTTTT+TGG | - | 43366:128561-128580 | None:intergenic | 35.0% |
| ! | GAAGATGATGATGTAGTTGT+TGG | + | 43366:128243-128262 | MS.gene81936:CDS | 35.0% |
| ! | TCATATAATGGGATACATGG+TGG | + | 43366:128715-128734 | MS.gene81936:CDS | 35.0% |
| !! | AAATGTCTATTTTGGGCAGA+TGG | + | 43366:128923-128942 | MS.gene81936:CDS | 35.0% |
| !! | AGTGAAGTTGATGAAAGTGA+AGG | + | 43366:128174-128193 | MS.gene81936:CDS | 35.0% |
| !! | GAGAGAAGAAGCATTTATCA+AGG | + | 43366:128462-128481 | MS.gene81936:CDS | 35.0% |
| !! | TTTTGGGTCTGTAATGAACA+CGG | - | 43366:128544-128563 | None:intergenic | 35.0% |
| AAAGGAATGTGGATCAGTTG+AGG | - | 43366:128870-128889 | None:intergenic | 40.0% | |
| AAGGGAATACATGTGATGAG+CGG | + | 43366:127992-128011 | MS.gene81936:CDS | 40.0% | |
| AGGGTGTAAATGCACAACAT+TGG | - | 43366:128624-128643 | None:intergenic | 40.0% | |
| AGTATGTATGCAACAAGCAG+GGG | + | 43366:128435-128454 | MS.gene81936:CDS | 40.0% | |
| AGTGAAGGTGATGAAAGTGA+AGG | + | 43366:128144-128163 | MS.gene81936:CDS | 40.0% | |
| ATGCGTGCTCTTATTAAGGA+TGG | + | 43366:128806-128825 | MS.gene81936:CDS | 40.0% | |
| CAGTATGTATGCAACAAGCA+GGG | + | 43366:128434-128453 | MS.gene81936:CDS | 40.0% | |
| GAATATTCTGCCACTGATGA+CGG | + | 43366:128893-128912 | MS.gene81936:CDS | 40.0% | |
| GATGAAAGTCAAGGTGATGA+TGG | + | 43366:128108-128127 | MS.gene81936:CDS | 40.0% | |
| GGAGACAATGAAAGTGATGA+TGG | + | 43366:128027-128046 | MS.gene81936:CDS | 40.0% | |
| GGTGATAAAGGTGAAGATGA+TGG | + | 43366:128075-128094 | MS.gene81936:CDS | 40.0% | |
| GTAGAACCGTAATCATGTTC+CGG | - | 43366:127826-127845 | None:intergenic | 40.0% | |
| TGATTACGGTTCTACAGATG+TGG | + | 43366:127831-127850 | MS.gene81936:CDS | 40.0% | |
| ! | ATTGATGGTCTTCAGTCACA+CGG | + | 43366:128680-128699 | MS.gene81936:CDS | 40.0% |
| !! | CTTTACGGATTTTGGTACAC+CGG | + | 43366:127804-127823 | MS.gene81936:CDS | 40.0% |
| !! | GACAATGAAAGTGATGATGG+TGG | + | 43366:128030-128049 | MS.gene81936:CDS | 40.0% |
| !! | TTATTTCTGCTTTGGCGATG+TGG | + | 43366:128961-128980 | MS.gene81936:CDS | 40.0% |
| ACTTTGTACGAGTGAGACGT+CGG | - | 43366:128508-128527 | None:intergenic | 45.0% | |
| GATGAGCGGTATGATGAAAC+TGG | + | 43366:128006-128025 | MS.gene81936:CDS | 45.0% | |
| GCAGTATGTATGCAACAAGC+AGG | + | 43366:128433-128452 | MS.gene81936:CDS | 45.0% | |
| GGTACACCGGAACATGATTA+CGG | + | 43366:127817-127836 | MS.gene81936:CDS | 45.0% | |
| GGTGAACGTGATGAAAGTGA+AGG | + | 43366:128129-128148 | MS.gene81936:CDS | 45.0% | |
| GGTGATGAAAGTGAAGGTCA+TGG | + | 43366:128150-128169 | MS.gene81936:CDS | 45.0% | |
| TATCCGATCATTGTTGGGAG+AGG | + | 43366:127871-127890 | MS.gene81936:CDS | 45.0% | |
| TTCTTAGTGAACCCAACACC+AGG | - | 43366:128758-128777 | None:intergenic | 45.0% | |
| TTTCAAACGTCCGTCATCAG+TGG | - | 43366:128906-128925 | None:intergenic | 45.0% | |
| ! | ACATTGGTTGGAGTAGGTTC+AGG | - | 43366:128608-128627 | None:intergenic | 45.0% |
| ! | AGTGAAGGTCATGGTAGTGA+AGG | + | 43366:128159-128178 | MS.gene81936:CDS | 45.0% |
| ! | CATTGGTTGGAGTAGGTTCA+GGG | - | 43366:128607-128626 | None:intergenic | 45.0% |
| ! | GATGCTGATGATGTGAGTCA+TGG | + | 43366:127949-127968 | MS.gene81936:CDS | 45.0% |
| ! | GTTGTTGGTGAGAAATGGTG+AGG | + | 43366:128258-128277 | MS.gene81936:intron | 45.0% |
| ! | TGCACAACATTGGTTGGAGT+AGG | - | 43366:128614-128633 | None:intergenic | 45.0% |
| !! | AGTGATGATGGTGGTTGTTC+TGG | + | 43366:128039-128058 | MS.gene81936:CDS | 45.0% |
| AAGGGTGGATATCCTGGTGT+TGG | + | 43366:128743-128762 | MS.gene81936:CDS | 50.0% | |
| AGGGTGGATATCCTGGTGTT+GGG | + | 43366:128744-128763 | MS.gene81936:CDS | 50.0% | |
| CCACCTCTCCCAACAATGAT+CGG | - | 43366:127877-127896 | None:intergenic | 50.0% | |
| GGATACATGGTGGCTCAGAA+GGG | + | 43366:128725-128744 | MS.gene81936:CDS | 50.0% | |
| TAATGAACACGGAGCCTAGC+TGG | - | 43366:128533-128552 | None:intergenic | 50.0% | |
| ! | CTCGTACAAAGTGTCCAGCT+AGG | + | 43366:128516-128535 | MS.gene81936:CDS | 50.0% |
| CCGATCATTGTTGGGAGAGG+TGG | + | 43366:127874-127893 | MS.gene81936:CDS | 55.0% | |
| GCTCAGAAGGGTGGATATCC+TGG | + | 43366:128737-128756 | MS.gene81936:CDS | 55.0% | |
| GGGATACATGGTGGCTCAGA+AGG | + | 43366:128724-128743 | MS.gene81936:CDS | 55.0% | |
| TACATGGTGGCTCAGAAGGG+TGG | + | 43366:128728-128747 | MS.gene81936:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43366 | gene | 127784 | 129024 | 127784 | ID=MS.gene81936 |
| 43366 | mRNA | 127784 | 129024 | 127784 | ID=MS.gene81936.t1;Parent=MS.gene81936 |
| 43366 | exon | 127784 | 128274 | 127784 | ID=MS.gene81936.t1.exon1;Parent=MS.gene81936.t1 |
| 43366 | CDS | 127784 | 128274 | 127784 | ID=cds.MS.gene81936.t1;Parent=MS.gene81936.t1 |
| 43366 | exon | 128388 | 129024 | 128388 | ID=MS.gene81936.t1.exon2;Parent=MS.gene81936.t1 |
| 43366 | CDS | 128388 | 129024 | 128388 | ID=cds.MS.gene81936.t1;Parent=MS.gene81936.t1 |
| Gene Sequence |
| Protein sequence |
