AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81974

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81974.t1 MTR_3g090430 97.697 304 6 1 1 303 29 332 0.0 613
MS.gene81974.t1 MTR_1g056810 57.353 136 37 4 13 128 3 137 2.19e-42 147
MS.gene81974.t1 MTR_2g093220 83.871 62 10 0 16 77 33 94 2.07e-32 121
MS.gene81974.t1 MTR_3g091820 72.973 74 18 1 4 77 26 97 7.87e-32 120
MS.gene81974.t1 MTR_4g461080 73.239 71 19 0 5 75 11 81 5.24e-30 115
MS.gene81974.t1 MTR_4g088580 85.455 55 8 0 25 79 57 111 7.44e-30 117
MS.gene81974.t1 MTR_2g096740 62.338 77 28 1 25 101 79 154 2.02e-29 114
MS.gene81974.t1 MTR_2g096740 67.143 70 23 0 17 86 56 125 2.60e-29 113
MS.gene81974.t1 MTR_8g027295 77.419 62 12 1 16 77 19 78 3.47e-29 112
MS.gene81974.t1 MTR_7g059400 64.865 74 26 0 4 77 57 130 3.75e-29 114
MS.gene81974.t1 MTR_4g022370 64.865 74 26 0 4 77 63 136 5.64e-29 114
MS.gene81974.t1 MTR_8g479350 76.667 60 14 0 25 84 70 129 6.86e-29 113
MS.gene81974.t1 MTR_7g024670 74.242 66 14 1 22 84 76 141 7.22e-29 114
MS.gene81974.t1 MTR_4g063780 63.514 74 27 0 4 77 56 129 9.95e-29 112
MS.gene81974.t1 MTR_1g077600 37.576 165 90 3 2 157 14 174 1.36e-28 111
MS.gene81974.t1 MTR_1g077600 37.576 165 90 3 2 157 14 174 1.43e-28 111
MS.gene81974.t1 MTR_8g068210 73.333 60 16 0 20 79 29 88 3.64e-28 111
MS.gene81974.t1 MTR_3g077750 75.862 58 14 0 16 73 54 111 6.64e-28 110
MS.gene81974.t1 MTR_2g014060 86.000 50 7 0 25 74 27 76 2.64e-27 108
MS.gene81974.t1 MTR_5g031440 85.714 49 7 0 25 73 45 93 8.03e-27 107
MS.gene81974.t1 MTR_4g109980 87.755 49 6 0 25 73 42 90 2.45e-26 106
MS.gene81974.t1 MTR_8g079060 56.962 79 34 0 25 103 31 109 8.70e-26 102
MS.gene81974.t1 MTR_8g015840 82.000 50 9 0 25 74 22 71 1.13e-25 102
MS.gene81974.t1 MTR_4g089095 76.786 56 12 1 24 79 51 105 1.19e-25 103
MS.gene81974.t1 MTR_2g059540 82.353 51 9 0 21 71 29 79 1.31e-25 101
MS.gene81974.t1 MTR_4g089095 76.786 56 12 1 24 79 17 71 1.56e-25 102
MS.gene81974.t1 MTR_2g014170 84.000 50 8 0 22 71 44 93 1.99e-25 103
MS.gene81974.t1 MTR_2g013370 74.138 58 14 1 22 79 45 101 2.13e-25 102
MS.gene81974.t1 MTR_3g435480 73.077 52 14 0 25 76 138 189 4.47e-24 102
MS.gene81974.t1 MTR_7g010950 68.421 57 17 1 20 76 133 188 7.32e-23 98.6
MS.gene81974.t1 MTR_6g012450 71.154 52 15 0 25 76 138 189 9.33e-23 98.2
MS.gene81974.t1 MTR_2g016030 65.517 58 20 0 19 76 43 100 3.61e-22 91.3
MS.gene81974.t1 MTR_4g082060 59.155 71 27 2 6 76 107 175 4.27e-22 96.3
MS.gene81974.t1 MTR_5g041420 56.338 71 29 2 6 76 123 191 6.39e-22 94.0
MS.gene81974.t1 MTR_7g086780 67.308 52 17 0 25 76 101 152 2.33e-21 94.0
MS.gene81974.t1 MTR_8g044220 64.151 53 19 0 25 77 120 172 2.36e-21 94.0
MS.gene81974.t1 MTR_6g027450 53.425 73 32 1 5 77 73 143 6.48e-21 91.3
MS.gene81974.t1 MTR_6g027460 54.167 72 31 1 5 76 80 149 8.93e-21 91.7
MS.gene81974.t1 MTR_5g041530 63.333 60 21 1 21 80 139 197 1.12e-20 91.3
MS.gene81974.t1 MTR_5g041380 62.712 59 18 1 22 76 122 180 9.89e-20 88.6
MS.gene81974.t1 MTR_5g041400 62.712 59 18 1 22 76 114 172 1.03e-19 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81974.t1 AT3G52440 61.864 118 31 3 15 127 13 121 2.85e-43 149
MS.gene81974.t1 AT3G52440 61.864 118 31 3 15 127 32 140 4.53e-43 149
MS.gene81974.t1 AT1G21340 42.222 225 105 10 3 215 15 226 3.82e-39 138
MS.gene81974.t1 AT5G60200 53.571 112 50 1 20 129 52 163 1.07e-34 127
MS.gene81974.t1 AT2G28510 87.500 56 7 0 22 77 47 102 3.74e-31 118
MS.gene81974.t1 AT4G24060 79.032 62 13 0 16 77 46 107 5.23e-31 119
MS.gene81974.t1 AT3G61850 87.037 54 7 0 24 77 75 128 3.36e-30 116
MS.gene81974.t1 AT3G61850 87.037 54 7 0 24 77 63 116 3.37e-30 115
MS.gene81974.t1 AT3G61850 87.037 54 7 0 24 77 63 116 3.37e-30 115
MS.gene81974.t1 AT2G37590 78.333 60 13 0 20 79 86 145 3.84e-30 116
MS.gene81974.t1 AT3G61850 87.037 54 7 0 24 77 63 116 4.06e-30 116
MS.gene81974.t1 AT3G61850 87.037 54 7 0 24 77 75 128 5.47e-30 116
MS.gene81974.t1 AT3G55370 75.000 64 16 0 17 80 70 133 9.68e-30 115
MS.gene81974.t1 AT3G55370 75.000 64 16 0 17 80 70 133 1.02e-29 115
MS.gene81974.t1 AT3G55370 75.000 64 16 0 17 80 115 178 1.15e-29 116
MS.gene81974.t1 AT1G07640 82.143 56 9 1 25 79 79 134 1.95e-29 114
MS.gene81974.t1 AT1G07640 82.143 56 9 1 25 79 87 142 2.48e-29 114
MS.gene81974.t1 AT1G07640 82.143 56 9 1 25 79 23 78 2.56e-29 113
MS.gene81974.t1 AT3G45610 49.107 112 51 3 20 126 37 147 7.17e-29 111
MS.gene81974.t1 AT5G02460 73.770 61 16 0 17 77 89 149 1.56e-28 113
MS.gene81974.t1 AT4G21050 57.831 83 35 0 25 107 26 108 2.08e-28 108
MS.gene81974.t1 AT5G60850 82.143 56 10 0 25 80 53 108 2.45e-28 111
MS.gene81974.t1 AT4G00940 85.185 54 8 0 24 77 69 122 3.28e-28 110
MS.gene81974.t1 AT4G00940 85.185 54 8 0 24 77 69 122 3.28e-28 110
MS.gene81974.t1 AT4G00940 85.185 54 8 0 24 77 69 122 3.28e-28 110
MS.gene81974.t1 AT5G65590 81.132 53 10 0 25 77 43 95 3.59e-28 111
MS.gene81974.t1 AT2G28810 76.364 55 13 0 20 74 74 128 1.07e-27 110
MS.gene81974.t1 AT1G28310 81.818 55 10 0 25 79 29 83 1.24e-27 109
MS.gene81974.t1 AT2G28810 76.364 55 13 0 20 74 91 145 1.61e-27 109
MS.gene81974.t1 AT1G28310 81.818 55 10 0 25 79 53 107 1.72e-27 109
MS.gene81974.t1 AT5G62940 87.755 49 6 0 25 73 75 123 3.83e-27 109
MS.gene81974.t1 AT2G46590 91.489 47 4 0 24 70 69 115 5.51e-27 108
MS.gene81974.t1 AT1G51700 56.471 85 31 2 25 103 35 119 6.08e-27 104
MS.gene81974.t1 AT2G46590 91.489 47 4 0 24 70 81 127 6.60e-27 108
MS.gene81974.t1 AT3G21270 86.275 51 7 0 25 75 31 81 8.82e-27 104
MS.gene81974.t1 AT1G64620 67.143 70 20 1 2 71 31 97 1.66e-26 107
MS.gene81974.t1 AT3G50410 77.358 53 12 0 25 77 32 84 2.41e-25 102
MS.gene81974.t1 AT4G38000 70.175 57 17 0 17 73 35 91 1.67e-24 99.8
MS.gene81974.t1 AT5G66940 70.370 54 16 0 24 77 33 86 9.47e-24 97.1
MS.gene81974.t1 AT1G69570 64.706 68 22 1 9 76 120 185 1.24e-23 100
MS.gene81974.t1 AT2G34140 71.154 52 15 0 25 76 60 111 3.80e-23 94.0
MS.gene81974.t1 AT1G29160 68.421 57 17 1 20 76 60 115 4.57e-23 94.0
MS.gene81974.t1 AT1G47655 78.723 47 10 0 25 71 31 77 6.13e-23 94.7
MS.gene81974.t1 AT3G47500 71.154 52 15 0 25 76 112 163 7.18e-23 98.2
MS.gene81974.t1 AT4G21040 75.000 52 13 0 25 76 27 78 7.86e-23 94.7
MS.gene81974.t1 AT1G26790 57.746 71 29 1 6 76 95 164 2.61e-22 95.9
MS.gene81974.t1 AT5G62430 54.930 71 31 1 6 76 38 107 3.98e-22 94.4
MS.gene81974.t1 AT4G21080 71.154 52 15 0 25 76 27 78 1.02e-21 92.0
MS.gene81974.t1 AT5G39660 71.154 52 15 0 25 76 140 191 1.09e-21 95.1
MS.gene81974.t1 AT5G39660 71.154 52 15 0 25 76 140 191 1.09e-21 95.1
MS.gene81974.t1 AT5G39660 71.154 52 15 0 25 76 140 191 1.09e-21 95.1
MS.gene81974.t1 AT4G21030 43.373 83 47 0 25 107 23 105 1.67e-15 73.9

Find 73 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATGAGGTTCATAATTA+TGG 0.189418 3.2:-76479663 MS.gene81974:CDS
TCACCATGATGAGTGCTATT+TGG 0.209784 3.2:+76479819 None:intergenic
GATGAGTGCTATTTGGATTT+TGG 0.211191 3.2:+76479826 None:intergenic
ATTATTGTAGTAACAGAATT+TGG 0.230505 3.2:+76480097 None:intergenic
GCAAAGGTTGTAGACGTTAT+TGG 0.239568 3.2:-76480046 MS.gene81974:CDS
TTCTCCGTTTCAACGCTTTC+GGG 0.242314 3.2:+76479714 None:intergenic
TAAGTGAAAGTTGGAATTCT+TGG 0.244522 3.2:-76479359 MS.gene81974:CDS
ATTCTCCGTTTCAACGCTTT+CGG 0.269550 3.2:+76479713 None:intergenic
GACCGTCTTTCCGCGAATTA+TGG 0.270747 3.2:-76479936 MS.gene81974:CDS
GATCAGTTTGAAGGGTTTCT+AGG 0.272563 3.2:-76479603 MS.gene81974:CDS
TAAATTGACACTAGTAGAAT+TGG 0.302955 3.2:+76479383 None:intergenic
GGTAATTCCATTTGTATCAT+TGG 0.309748 3.2:+76479477 None:intergenic
GAAGCACAACGAGGACAATT+AGG 0.359511 3.2:+76480128 None:intergenic
GAGAGCGAGGCTAATAATAA+TGG 0.378005 3.2:-76479780 MS.gene81974:CDS
TTGTTGCCATGTATAATTAT+TGG 0.378408 3.2:+76479506 None:intergenic
TCATCATGGTGATGAGGAAT+TGG 0.386158 3.2:-76479808 MS.gene81974:CDS
TATCACGCGAACCATTGTTT+TGG 0.390102 3.2:+76479883 None:intergenic
AAGTGAAAGTTGGAATTCTT+GGG 0.392557 3.2:-76479358 MS.gene81974:CDS
TGACAAATTGTTGATATTGT+TGG 0.392892 3.2:+76479743 None:intergenic
ATTTGTCCATTGAGGTTGTT+GGG 0.398492 3.2:+76480186 None:intergenic
TCAATTTAATAAGTGAAAGT+TGG 0.399372 3.2:-76479368 MS.gene81974:CDS
ATGTGGTACAAGATGTTCTA+TGG 0.409257 3.2:-76479569 MS.gene81974:CDS
TGGACAAATCAAATTCAAAC+TGG 0.417202 3.2:-76480172 MS.gene81974:CDS
TCCATTGAGGTTGTTGGGAA+TGG 0.426025 3.2:+76480191 None:intergenic
TTGCGGCAGCCACCGCCAAC+GGG 0.427583 3.2:+76479996 None:intergenic
CATCAAAAGGTTTCTGAGGC+TGG 0.436350 3.2:+76479679 None:intergenic
ATTGAATATGATCAGTTTGA+AGG 0.437833 3.2:-76479612 MS.gene81974:CDS
ATTACCAGGAGAGTTGGAAT+TGG 0.443432 3.2:-76479460 MS.gene81974:CDS
GGACCAAAGGTGGCTCTCTT+CGG 0.446025 3.2:-76480025 MS.gene81974:CDS
ACCACCACCAATGATACAAA+TGG 0.457520 3.2:-76479484 MS.gene81974:CDS
CTTCGGAATGTTCCCGTTGG+CGG 0.470284 3.2:-76480008 MS.gene81974:CDS
TAATTATGAACCTCATCAAA+AGG 0.476723 3.2:+76479666 None:intergenic
CGGAATGTTCCCGTTGGCGG+TGG 0.477735 3.2:-76480005 MS.gene81974:CDS
GTACCATTCTCATTATTCAA+TGG 0.481490 3.2:+76479414 None:intergenic
TTTGCGGCAGCCACCGCCAA+CGG 0.485880 3.2:+76479995 None:intergenic
ATTCCGAAGAGAGCCACCTT+TGG 0.486871 3.2:+76480022 None:intergenic
ATATTCATAGCATTAGAATC+AGG 0.491964 3.2:+76479546 None:intergenic
TCCATTTGTATCATTGGTGG+TGG 0.492349 3.2:+76479483 None:intergenic
GCCATTCCCAACAACCTCAA+TGG 0.494342 3.2:-76480192 MS.gene81974:CDS
CTTCTTCCAATAATTATACA+TGG 0.509902 3.2:-76479512 MS.gene81974:CDS
GATGAGGTTCATAATTATGG+AGG 0.510864 3.2:-76479660 MS.gene81974:CDS
AATGGAATTACCAGGAGAGT+TGG 0.513056 3.2:-76479466 MS.gene81974:CDS
GACACCCGAAAGCGTTGAAA+CGG 0.518206 3.2:-76479718 MS.gene81974:CDS
AATTCCATTTGTATCATTGG+TGG 0.522083 3.2:+76479480 None:intergenic
ATCAGCAGAGCCATAATTCG+CGG 0.525005 3.2:+76479926 None:intergenic
TTGCCATTGAATAATGAGAA+TGG 0.531446 3.2:-76479417 MS.gene81974:CDS
TTGAATATGATCAGTTTGAA+GGG 0.531887 3.2:-76479611 MS.gene81974:CDS
GATTTGTCCATTGAGGTTGT+TGG 0.532932 3.2:+76480185 None:intergenic
TTCTGACCGCCTTTCCACGA+CGG 0.537387 3.2:+76479970 None:intergenic
TGAATTTGATTTGTCCATTG+AGG 0.539035 3.2:+76480178 None:intergenic
TATTCCAATTCCAACTCTCC+TGG 0.541184 3.2:+76479456 None:intergenic
TTCTCATTATTCAATGGCAA+TGG 0.542279 3.2:+76479420 None:intergenic
ATGAGGTTCATAATTATGGA+GGG 0.547185 3.2:-76479659 MS.gene81974:CDS
TCTCTTCGGAATGTTCCCGT+TGG 0.557776 3.2:-76480011 MS.gene81974:CDS
AGCCATAATTCGCGGAAAGA+CGG 0.562712 3.2:+76479934 None:intergenic
AATCCAAATAGCACTCATCA+TGG 0.568770 3.2:-76479822 MS.gene81974:CDS
TTATTCAATGGCAATGGCAA+TGG 0.570708 3.2:+76479426 None:intergenic
ACCTCATCAAAAGGTTTCTG+AGG 0.573056 3.2:+76479675 None:intergenic
CAACCACGGTATTTCTGCAA+AGG 0.575852 3.2:-76480062 MS.gene81974:CDS
TGTAGACGTTATTGGACCAA+AGG 0.588786 3.2:-76480038 MS.gene81974:CDS
AGACGTTATTGGACCAAAGG+TGG 0.608980 3.2:-76480035 MS.gene81974:CDS
TGAGGTTCATAATTATGGAG+GGG 0.616763 3.2:-76479658 MS.gene81974:CDS
TAGCACTCATCATGGTGATG+AGG 0.617266 3.2:-76479814 MS.gene81974:CDS
TCTAGGTGTTGATGAAGATG+TGG 0.622739 3.2:-76479586 MS.gene81974:CDS
TTAGGAGCTATCTCAATGTG+TGG 0.628107 3.2:+76480146 None:intergenic
GGAATTGGAAATTGAGAGCG+AGG 0.633302 3.2:-76479793 MS.gene81974:CDS
CATCAGCAAAACCAAAACAA+TGG 0.640773 3.2:-76479894 MS.gene81974:CDS
ATGATACAAATGGAATTACC+AGG 0.647801 3.2:-76479474 MS.gene81974:CDS
TGAAACGGAGAATGATGCAG+TGG 0.672585 3.2:-76479703 MS.gene81974:CDS
CAACCTTTGCAGAAATACCG+TGG 0.687599 3.2:+76480059 None:intergenic
TGGTTCGCGTGATATAGACA+TGG 0.703930 3.2:-76479874 MS.gene81974:CDS
ATTATAGCTTGTCACAACCA+CGG 0.706519 3.2:-76480076 MS.gene81974:CDS
GTGTTTGATGAAGCACAACG+AGG 0.730421 3.2:+76480119 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTGTAGTAACAGAATT+TGG + chr3.2:76479405-76479424 None:intergenic 20.0%
!! TCAATTTAATAAGTGAAAGT+TGG - chr3.2:76480131-76480150 MS.gene81974:CDS 20.0%
! ATATTCATAGCATTAGAATC+AGG + chr3.2:76479956-76479975 None:intergenic 25.0%
! ATTGAATATGATCAGTTTGA+AGG - chr3.2:76479887-76479906 MS.gene81974:CDS 25.0%
! CTTCTTCCAATAATTATACA+TGG - chr3.2:76479987-76480006 MS.gene81974:CDS 25.0%
! TAAATTGACACTAGTAGAAT+TGG + chr3.2:76480119-76480138 None:intergenic 25.0%
! TAATTATGAACCTCATCAAA+AGG + chr3.2:76479836-76479855 None:intergenic 25.0%
! TGACAAATTGTTGATATTGT+TGG + chr3.2:76479759-76479778 None:intergenic 25.0%
! TTGAATATGATCAGTTTGAA+GGG - chr3.2:76479888-76479907 MS.gene81974:CDS 25.0%
! TTGTTGCCATGTATAATTAT+TGG + chr3.2:76479996-76480015 None:intergenic 25.0%
! TTTGATGAGGTTCATAATTA+TGG - chr3.2:76479836-76479855 MS.gene81974:CDS 25.0%
!! TTCTTTTGATCTTTCAACTA+TGG - chr3.2:76480165-76480184 MS.gene81974:CDS 25.0%
!!! TGGATTTTGGTTTAAGAATT+TGG + chr3.2:76479663-76479682 None:intergenic 25.0%
AAGTGAAAGTTGGAATTCTT+GGG - chr3.2:76480141-76480160 MS.gene81974:CDS 30.0%
AATTCCATTTGTATCATTGG+TGG + chr3.2:76480022-76480041 None:intergenic 30.0%
ATGAGGTTCATAATTATGGA+GGG - chr3.2:76479840-76479859 MS.gene81974:CDS 30.0%
ATGATACAAATGGAATTACC+AGG - chr3.2:76480025-76480044 MS.gene81974:CDS 30.0%
GGTAATTCCATTTGTATCAT+TGG + chr3.2:76480025-76480044 None:intergenic 30.0%
GTACCATTCTCATTATTCAA+TGG + chr3.2:76480088-76480107 None:intergenic 30.0%
TAAGTGAAAGTTGGAATTCT+TGG - chr3.2:76480140-76480159 MS.gene81974:CDS 30.0%
TGGACAAATCAAATTCAAAC+TGG - chr3.2:76479327-76479346 MS.gene81974:CDS 30.0%
TTCTCATTATTCAATGGCAA+TGG + chr3.2:76480082-76480101 None:intergenic 30.0%
TTGCCATTGAATAATGAGAA+TGG - chr3.2:76480082-76480101 MS.gene81974:CDS 30.0%
!! TGAATTTGATTTGTCCATTG+AGG + chr3.2:76479324-76479343 None:intergenic 30.0%
AATCCAAATAGCACTCATCA+TGG - chr3.2:76479677-76479696 MS.gene81974:CDS 35.0%
ATGTGGTACAAGATGTTCTA+TGG - chr3.2:76479930-76479949 MS.gene81974:CDS 35.0%
ATTTGTCCATTGAGGTTGTT+GGG + chr3.2:76479316-76479335 None:intergenic 35.0%
CATCAGCAAAACCAAAACAA+TGG - chr3.2:76479605-76479624 MS.gene81974:CDS 35.0%
GATGAGGTTCATAATTATGG+AGG - chr3.2:76479839-76479858 MS.gene81974:CDS 35.0%
TGAGGTTCATAATTATGGAG+GGG - chr3.2:76479841-76479860 MS.gene81974:CDS 35.0%
TTATTCAATGGCAATGGCAA+TGG + chr3.2:76480076-76480095 None:intergenic 35.0%
! ATTATAGCTTGTCACAACCA+CGG - chr3.2:76479423-76479442 MS.gene81974:CDS 35.0%
!!! GATGAGTGCTATTTGGATTT+TGG + chr3.2:76479676-76479695 None:intergenic 35.0%
AATGGAATTACCAGGAGAGT+TGG - chr3.2:76480033-76480052 MS.gene81974:CDS 40.0%
ACCACCACCAATGATACAAA+TGG - chr3.2:76480015-76480034 MS.gene81974:CDS 40.0%
ACCTCATCAAAAGGTTTCTG+AGG + chr3.2:76479827-76479846 None:intergenic 40.0%
ATTACCAGGAGAGTTGGAAT+TGG - chr3.2:76480039-76480058 MS.gene81974:CDS 40.0%
ATTCTCCGTTTCAACGCTTT+CGG + chr3.2:76479789-76479808 None:intergenic 40.0%
GAGAGCGAGGCTAATAATAA+TGG - chr3.2:76479719-76479738 MS.gene81974:CDS 40.0%
GATCAGTTTGAAGGGTTTCT+AGG - chr3.2:76479896-76479915 MS.gene81974:CDS 40.0%
GATTTGTCCATTGAGGTTGT+TGG + chr3.2:76479317-76479336 None:intergenic 40.0%
GCAAAGGTTGTAGACGTTAT+TGG - chr3.2:76479453-76479472 MS.gene81974:CDS 40.0%
TATTCCAATTCCAACTCTCC+TGG + chr3.2:76480046-76480065 None:intergenic 40.0%
TCCATTTGTATCATTGGTGG+TGG + chr3.2:76480019-76480038 None:intergenic 40.0%
TGTAGACGTTATTGGACCAA+AGG - chr3.2:76479461-76479480 MS.gene81974:CDS 40.0%
TTAGGAGCTATCTCAATGTG+TGG + chr3.2:76479356-76479375 None:intergenic 40.0%
! TATCACGCGAACCATTGTTT+TGG + chr3.2:76479619-76479638 None:intergenic 40.0%
! TCACCATGATGAGTGCTATT+TGG + chr3.2:76479683-76479702 None:intergenic 40.0%
! TCATCATGGTGATGAGGAAT+TGG - chr3.2:76479691-76479710 MS.gene81974:CDS 40.0%
!! TCTAGGTGTTGATGAAGATG+TGG - chr3.2:76479913-76479932 MS.gene81974:CDS 40.0%
AGACGTTATTGGACCAAAGG+TGG - chr3.2:76479464-76479483 MS.gene81974:CDS 45.0%
AGCCATAATTCGCGGAAAGA+CGG + chr3.2:76479568-76479587 None:intergenic 45.0%
ATCAGCAGAGCCATAATTCG+CGG + chr3.2:76479576-76479595 None:intergenic 45.0%
CAACCTTTGCAGAAATACCG+TGG + chr3.2:76479443-76479462 None:intergenic 45.0%
GAAGCACAACGAGGACAATT+AGG + chr3.2:76479374-76479393 None:intergenic 45.0%
GGAATTGGAAATTGAGAGCG+AGG - chr3.2:76479706-76479725 MS.gene81974:CDS 45.0%
GTGTTTGATGAAGCACAACG+AGG + chr3.2:76479383-76479402 None:intergenic 45.0%
TCCATTGAGGTTGTTGGGAA+TGG + chr3.2:76479311-76479330 None:intergenic 45.0%
TGAAACGGAGAATGATGCAG+TGG - chr3.2:76479796-76479815 MS.gene81974:CDS 45.0%
TGGTTCGCGTGATATAGACA+TGG - chr3.2:76479625-76479644 MS.gene81974:CDS 45.0%
TTCTCCGTTTCAACGCTTTC+GGG + chr3.2:76479788-76479807 None:intergenic 45.0%
! CAACCACGGTATTTCTGCAA+AGG - chr3.2:76479437-76479456 MS.gene81974:CDS 45.0%
! GCCTCAGAAACCTTTTGATG+AGG - chr3.2:76479823-76479842 MS.gene81974:CDS 45.0%
! TAGCACTCATCATGGTGATG+AGG - chr3.2:76479685-76479704 MS.gene81974:CDS 45.0%
!! CATCAAAAGGTTTCTGAGGC+TGG + chr3.2:76479823-76479842 None:intergenic 45.0%
ATTCCGAAGAGAGCCACCTT+TGG + chr3.2:76479480-76479499 None:intergenic 50.0%
CAAAAACCGTCGTGGAAAGG+CGG - chr3.2:76479523-76479542 MS.gene81974:CDS 50.0%
GACCGTCTTTCCGCGAATTA+TGG - chr3.2:76479563-76479582 MS.gene81974:CDS 50.0%
GCCATTCCCAACAACCTCAA+TGG - chr3.2:76479307-76479326 MS.gene81974:CDS 50.0%
TCTCTTCGGAATGTTCCCGT+TGG - chr3.2:76479488-76479507 MS.gene81974:CDS 50.0%
! CCTTTCCACGACGGTTTTTG+CGG + chr3.2:76479523-76479542 None:intergenic 50.0%
!! GACACCCGAAAGCGTTGAAA+CGG - chr3.2:76479781-76479800 MS.gene81974:CDS 50.0%
CCGCAAAAACCGTCGTGGAA+AGG - chr3.2:76479520-76479539 MS.gene81974:CDS 55.0%
CTTCGGAATGTTCCCGTTGG+CGG - chr3.2:76479491-76479510 MS.gene81974:CDS 55.0%
GGACCAAAGGTGGCTCTCTT+CGG - chr3.2:76479474-76479493 MS.gene81974:CDS 55.0%
TTCTGACCGCCTTTCCACGA+CGG + chr3.2:76479532-76479551 None:intergenic 55.0%
CGGAATGTTCCCGTTGGCGG+TGG - chr3.2:76479494-76479513 MS.gene81974:CDS 65.0%
GGCTGCCGCAAAAACCGTCG+TGG - chr3.2:76479515-76479534 MS.gene81974:CDS 65.0%
TTTGCGGCAGCCACCGCCAA+CGG + chr3.2:76479507-76479526 None:intergenic 65.0%
TTGCGGCAGCCACCGCCAAC+GGG + chr3.2:76479506-76479525 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 76479305 76480216 76479305 ID=MS.gene81974
chr3.2 mRNA 76479305 76480216 76479305 ID=MS.gene81974.t1;Parent=MS.gene81974
chr3.2 exon 76479305 76480216 76479305 ID=MS.gene81974.t1.exon1;Parent=MS.gene81974.t1
chr3.2 CDS 76479305 76480216 76479305 ID=cds.MS.gene81974.t1;Parent=MS.gene81974.t1
Gene Sequence

>MS.gene81974

ATGCCATTCCCAACAACCTCAATGGACAAATCAAATTCAAACTGGAAACCACACATTGAGATAGCTCCTAATTGTCCTCGTTGTGCTTCATCAAACACCAAATTCTGTTACTACAATAATTATAGCTTGTCACAACCACGGTATTTCTGCAAAGGTTGTAGACGTTATTGGACCAAAGGTGGCTCTCTTCGGAATGTTCCCGTTGGCGGTGGCTGCCGCAAAAACCGTCGTGGAAAGGCGGTCAGAAATTCTCAGACTGACCGTCTTTCCGCGAATTATGGCTCTGCTGATGATGAAGATCATCAGCAAAACCAAAACAATGGTTCGCGTGATATAGACATGGCTGTTGTTTTTGCCAAATTCTTAAACCAAAATCCAAATAGCACTCATCATGGTGATGAGGAATTGGAAATTGAGAGCGAGGCTAATAATAATGGTTTGTCTTCTTCTTCCAACAATATCAACAATTTGTCATCGACACCCGAAAGCGTTGAAACGGAGAATGATGCAGTGGTCCAGCCTCAGAAACCTTTTGATGAGGTTCATAATTATGGAGGGGAACATGAATTAAGTTTGAGTGCGATTGAATATGATCAGTTTGAAGGGTTTCTAGGTGTTGATGAAGATGTGGTACAAGATGTTCTATGGCCTGATTCTAATGCTATGAATATGATGATGTCATCTTCTTCCAATAATTATACATGGCAACAACCACCACCAATGATACAAATGGAATTACCAGGAGAGTTGGAATTGGAATACTCAATGCCATTGCCATTGCCATTGAATAATGAGAATGGTACTATTAATTCCAATTCTACTAGTGTCAATTTAATAAGTGAAAGTTGGAATTCTTGGGATTCTTTTGATCTTTCAACTATGGAAGTCTTCTCTTCAAGTTCAAGACCTTAA
Protein sequence

>MS.gene81974.t1

MPFPTTSMDKSNSNWKPHIEIAPNCPRCASSNTKFCYYNNYSLSQPRYFCKGCRRYWTKGGSLRNVPVGGGCRKNRRGKAVRNSQTDRLSANYGSADDEDHQQNQNNGSRDIDMAVVFAKFLNQNPNSTHHGDEELEIESEANNNGLSSSSNNINNLSSTPESVETENDAVVQPQKPFDEVHNYGGEHELSLSAIEYDQFEGFLGVDEDVVQDVLWPDSNAMNMMMSSSSNNYTWQQPPPMIQMELPGELELEYSMPLPLPLNNENGTINSNSTSVNLISESWNSWDSFDLSTMEVFSSSSRP