AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82030.t1 MTR_8g024770 94.466 253 14 0 1 253 202 454 1.68e-175 491
MS.gene82030.t1 MTR_8g024790 78.682 258 44 6 1 253 211 462 1.42e-134 387
MS.gene82030.t1 MTR_5g030430 51.485 202 89 4 59 253 474 673 5.44e-56 190
MS.gene82030.t1 MTR_8g067280 48.187 193 92 3 60 245 444 635 1.40e-51 177
MS.gene82030.t1 MTR_7g096350 40.000 200 76 5 27 225 319 475 6.11e-31 120
MS.gene82030.t1 MTR_8g027495 38.596 171 91 5 76 245 456 613 5.95e-26 106
MS.gene82030.t1 MTR_7g117670 35.465 172 95 3 76 245 400 557 4.32e-23 98.2
MS.gene82030.t1 MTR_3g104100 32.927 164 102 3 66 222 183 345 5.29e-18 82.8
MS.gene82030.t1 MTR_1g072320 31.579 209 115 6 43 234 432 629 6.12e-17 80.1
MS.gene82030.t1 MTR_4g009110 25.989 177 120 3 64 237 164 332 1.15e-16 78.6
MS.gene82030.t1 MTR_2g081040 33.548 155 95 3 75 222 174 327 6.84e-16 76.6
MS.gene82030.t1 MTR_4g009540 28.177 181 108 5 64 234 142 310 1.08e-15 75.5
MS.gene82030.t1 MTR_0250s0040 29.730 148 80 4 49 191 69 197 2.40e-15 73.9
MS.gene82030.t1 MTR_4g009540 28.177 181 108 5 64 234 142 310 2.50e-15 74.7
MS.gene82030.t1 MTR_3g030700 35.795 176 98 5 55 222 173 341 5.88e-15 73.9
MS.gene82030.t1 MTR_4g097940 29.193 161 99 4 64 218 138 289 1.88e-14 72.0
MS.gene82030.t1 MTR_7g083900 60.000 55 22 0 76 130 284 338 7.27e-14 70.9
MS.gene82030.t1 MTR_7g083900 60.000 55 22 0 76 130 284 338 7.42e-14 70.9
MS.gene82030.t1 MTR_3g498695 58.182 55 23 0 76 130 296 350 1.14e-13 70.5
MS.gene82030.t1 MTR_3g498695 58.182 55 23 0 76 130 296 350 1.15e-13 70.5
MS.gene82030.t1 MTR_5g014640 31.944 144 83 3 79 218 155 287 2.17e-13 68.9
MS.gene82030.t1 MTR_2g022280 26.786 168 106 5 77 231 1 164 3.61e-13 66.6
MS.gene82030.t1 MTR_4g097950 32.624 141 82 4 53 190 55 185 7.47e-13 66.6
MS.gene82030.t1 MTR_4g066460 30.263 152 72 5 45 191 139 261 1.16e-12 67.0
MS.gene82030.t1 MTR_4g067010 28.788 132 74 4 65 191 137 253 1.19e-12 67.0
MS.gene82030.t1 MTR_6g047570 30.159 126 74 3 71 191 152 268 1.99e-12 66.2
MS.gene82030.t1 MTR_8g098275 56.364 55 24 0 77 131 307 361 5.02e-12 65.5
MS.gene82030.t1 MTR_8g098275 56.364 55 24 0 77 131 243 297 5.07e-12 65.5
MS.gene82030.t1 MTR_8g098275 56.364 55 24 0 77 131 400 454 5.14e-12 65.9
MS.gene82030.t1 MTR_4g092700 38.372 86 48 2 46 131 131 211 7.01e-12 64.7
MS.gene82030.t1 MTR_2g084280 29.801 151 90 6 82 227 137 276 9.00e-12 64.3
MS.gene82030.t1 MTR_2g038040 50.000 62 24 1 79 133 53 114 9.45e-12 63.5
MS.gene82030.t1 MTR_2g104490 26.630 184 114 8 61 232 116 290 1.12e-11 63.9
MS.gene82030.t1 MTR_4g097920 29.167 168 98 7 57 220 121 271 1.79e-11 63.5
MS.gene82030.t1 MTR_7g110810 31.624 117 69 2 28 134 153 268 2.00e-11 63.5
MS.gene82030.t1 MTR_4g097920 29.167 168 98 7 57 220 136 286 2.02e-11 63.2
MS.gene82030.t1 MTR_0246s0020 28.814 118 75 2 78 190 198 311 2.05e-11 63.5
MS.gene82030.t1 MTR_0246s0020 28.814 118 75 2 78 190 198 311 2.22e-11 63.5
MS.gene82030.t1 MTR_2g104490 26.667 180 111 8 61 228 116 286 2.22e-11 63.2
MS.gene82030.t1 MTR_0011s0260 27.778 144 94 3 78 218 183 319 2.24e-11 63.5
MS.gene82030.t1 MTR_2g104550 20.707 198 146 5 48 239 104 296 2.43e-11 63.2
MS.gene82030.t1 MTR_0011s0210 31.624 117 70 3 78 191 183 292 2.54e-11 63.2
MS.gene82030.t1 MTR_2g010450 25.904 166 102 3 78 234 177 330 3.19e-11 62.8
MS.gene82030.t1 MTR_4g094762 30.769 156 94 6 79 224 293 444 3.34e-11 63.2
MS.gene82030.t1 MTR_1g112680 50.000 50 25 0 82 131 321 370 3.79e-11 63.2
MS.gene82030.t1 MTR_4g057270 52.000 50 24 0 82 131 153 202 5.45e-11 62.0
MS.gene82030.t1 MTR_4g066380 40.476 84 33 2 45 128 137 203 9.22e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82030.t1 AT1G32640 51.485 202 80 6 58 253 433 622 5.67e-55 186
MS.gene82030.t1 AT4G17880 48.691 191 83 5 59 244 397 577 1.97e-52 179
MS.gene82030.t1 AT5G46760 47.980 198 95 5 59 253 396 588 1.07e-51 177
MS.gene82030.t1 AT5G46830 49.412 170 77 4 76 244 341 502 1.30e-39 144
MS.gene82030.t1 AT4G00870 49.123 171 79 3 77 244 248 413 1.18e-38 139
MS.gene82030.t1 AT2G46510 37.089 213 97 6 28 231 342 526 1.63e-31 122
MS.gene82030.t1 AT1G01260 38.372 172 79 4 62 233 417 561 1.77e-28 113
MS.gene82030.t1 AT1G01260 38.372 172 79 4 62 233 417 561 1.77e-28 113
MS.gene82030.t1 AT1G01260 38.372 172 79 4 62 233 417 561 1.77e-28 113
MS.gene82030.t1 AT4G16430 36.095 169 81 2 58 226 300 441 1.66e-27 110
MS.gene82030.t1 AT4G37850 29.954 217 120 7 24 218 84 290 1.92e-16 77.8
MS.gene82030.t1 AT4G37850 29.954 217 120 7 24 218 84 290 1.92e-16 77.8
MS.gene82030.t1 AT3G26744 60.000 55 22 0 76 130 305 359 1.52e-14 73.2
MS.gene82030.t1 AT3G26744 60.000 55 22 0 76 130 305 359 1.52e-14 73.2
MS.gene82030.t1 AT3G26744 60.000 55 22 0 76 130 305 359 1.52e-14 73.2
MS.gene82030.t1 AT3G26744 60.000 55 22 0 76 130 305 359 1.57e-14 73.2
MS.gene82030.t1 AT1G12860 60.000 55 22 0 76 130 265 319 2.15e-14 72.4
MS.gene82030.t1 AT1G12860 60.000 55 22 0 76 130 265 319 2.49e-14 72.4
MS.gene82030.t1 AT5G57150 27.523 218 112 8 29 226 16 207 2.75e-13 67.4
MS.gene82030.t1 AT5G57150 27.523 218 112 8 29 226 16 207 3.67e-13 67.8
MS.gene82030.t1 AT5G57150 28.272 191 96 7 56 226 39 208 4.74e-13 67.0
MS.gene82030.t1 AT5G57150 28.272 191 96 7 56 226 39 208 5.23e-13 67.0
MS.gene82030.t1 AT1G63650 26.061 165 106 4 78 227 405 568 5.52e-13 68.6
MS.gene82030.t1 AT1G63650 26.061 165 106 4 78 227 405 568 5.52e-13 68.6
MS.gene82030.t1 AT1G63650 26.061 165 106 4 78 227 405 568 5.52e-13 68.6
MS.gene82030.t1 AT4G29930 62.222 45 17 0 79 123 55 99 6.75e-13 66.6
MS.gene82030.t1 AT4G29930 62.222 45 17 0 79 123 55 99 7.45e-13 66.6
MS.gene82030.t1 AT4G29930 62.222 45 17 0 79 123 55 99 7.52e-13 66.2
MS.gene82030.t1 AT4G09820 50.909 55 27 0 77 131 362 416 1.15e-12 67.8
MS.gene82030.t1 AT5G57150 27.979 193 98 7 54 226 37 208 1.16e-12 66.2
MS.gene82030.t1 AT4G29930 62.222 45 17 0 79 123 55 99 1.46e-12 66.2
MS.gene82030.t1 AT4G29930 62.222 45 17 0 79 123 55 99 1.46e-12 66.2
MS.gene82030.t1 AT4G29930 62.222 45 17 0 79 123 55 99 1.48e-12 65.9
MS.gene82030.t1 AT4G29930 63.636 44 16 0 80 123 56 99 2.07e-12 64.3
MS.gene82030.t1 AT2G16910 29.843 191 107 7 82 250 318 503 3.54e-12 66.2
MS.gene82030.t1 AT2G22750 36.842 133 75 4 65 190 137 267 2.69e-11 63.2
MS.gene82030.t1 AT2G22750 36.842 133 75 4 65 190 137 267 2.75e-11 63.2
MS.gene82030.t1 AT2G22750 36.842 133 75 4 65 190 109 239 2.95e-11 62.8
MS.gene82030.t1 AT2G22750 36.842 133 75 4 65 190 133 263 2.96e-11 62.8
MS.gene82030.t1 AT2G22750 36.842 133 75 4 65 190 109 239 2.99e-11 62.8
MS.gene82030.t1 AT5G41315 25.140 179 112 6 69 227 441 617 3.39e-11 63.2
MS.gene82030.t1 AT5G41315 25.140 179 112 6 69 227 438 614 3.61e-11 63.2
MS.gene82030.t1 AT5G41315 25.140 179 112 6 69 227 433 609 4.33e-11 63.2
MS.gene82030.t1 AT4G38070 31.579 114 73 2 19 130 37 147 5.24e-11 61.6
MS.gene82030.t1 AT3G24140 24.537 216 138 5 58 249 175 389 6.05e-11 62.4
MS.gene82030.t1 AT3G24140 24.537 216 138 5 58 249 175 389 6.09e-11 62.4
MS.gene82030.t1 AT1G10610 50.000 54 24 1 79 129 168 221 7.00e-11 62.0
MS.gene82030.t1 AT2G28160 36.735 98 50 2 34 131 99 184 7.62e-11 61.6
MS.gene82030.t1 AT1G10610 46.269 67 32 2 79 141 235 301 7.88e-11 62.0
MS.gene82030.t1 AT1G10610 46.269 67 32 2 79 141 265 331 8.72e-11 62.0
MS.gene82030.t1 AT2G28160 36.735 98 50 2 34 131 99 184 9.35e-11 61.2

Find 32 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATTGAAGATTTAAAATT+AGG 0.097958 8.4:-70295008 MS.gene82030:CDS
CTTGAAAGATTTGGAGTTTC+AGG 0.118528 8.4:-70294769 MS.gene82030:CDS
CTTCTCATGGGTGGCTTCTT+TGG 0.173047 8.4:+70295358 None:intergenic
GAGATTGATGTGAAGATTAT+AGG 0.182361 8.4:-70294858 MS.gene82030:CDS
TGAGAATTGCAGACCTAATT+AGG 0.229660 8.4:+70294655 None:intergenic
TACTACAACTAGGTTTGTTT+TGG 0.270219 8.4:+70294898 None:intergenic
TCTTCATTTCTCATCTCATT+AGG 0.290165 8.4:+70294681 None:intergenic
GACCGTTAATAAAGTTATAA+TGG 0.291909 8.4:-70294727 MS.gene82030:CDS
ATCTTAAAGCATAGAATCTA+TGG 0.304209 8.4:+70295111 None:intergenic
AGATTGAAGATTTAAAATTA+GGG 0.310294 8.4:-70295007 MS.gene82030:CDS
AACCATTATAACTTTATTAA+CGG 0.312837 8.4:+70294725 None:intergenic
TTATCCATCTTTGAAACGTT+TGG 0.324231 8.4:+70295080 None:intergenic
GCCAACATGGGTGTCGTCTC+TGG 0.337682 8.4:-70295389 None:intergenic
AATGAGTGTCTTGAAAGATT+TGG 0.347886 8.4:-70294778 MS.gene82030:CDS
TTTATCTCTCCTTTGTCTCT+CGG 0.386285 8.4:+70295139 None:intergenic
ATGGGTGGCTTCTTTGGCGC+TGG 0.396457 8.4:+70295364 None:intergenic
GGTTCCAAACGTTTCAAAGA+TGG 0.424360 8.4:-70295084 MS.gene82030:CDS
GAAATGAAGAGAGCCTAATT+AGG 0.443372 8.4:-70294668 MS.gene82030:CDS
TTTAAAATTAGGGCAACAAA+AGG 0.456499 8.4:-70294997 MS.gene82030:CDS
AAAAGAGAACCGAAGAAAAG+AGG 0.478932 8.4:-70295209 MS.gene82030:CDS
TCGGCTTCCACATGGTTCAT+CGG 0.486588 8.4:+70295158 None:intergenic
GGTGTTTGAGTTCGTGTCAA+CGG 0.501817 8.4:+70295179 None:intergenic
ATTCTATGCTTTAAGATCTG+TGG 0.527981 8.4:-70295105 MS.gene82030:CDS
ACACTCATTAATCGTGCACT+AGG 0.531276 8.4:+70294792 None:intergenic
TTCTTTGTTATCAGATGCAG+TGG 0.539939 8.4:-70295054 MS.gene82030:CDS
TTTGTCTCTCGGCTTCCACA+TGG 0.548386 8.4:+70295150 None:intergenic
ATGTGGAAGCCGAGAGACAA+AGG 0.579932 8.4:-70295148 MS.gene82030:CDS
ATAAGATTCTTACTACAACT+AGG 0.600771 8.4:+70294888 None:intergenic
GATTATAGGACATGATGCTA+TGG 0.615350 8.4:-70294844 MS.gene82030:CDS
CACTCATTAATCGTGCACTA+GGG 0.626623 8.4:+70294793 None:intergenic
TGCAACGACAACTTCAATCG+TGG 0.678130 8.4:-70294925 MS.gene82030:CDS
TCAAACACCGATGAACCATG+TGG 0.723026 8.4:-70295165 MS.gene82030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACCATTATAACTTTATTAA+CGG + chr8.4:70295305-70295324 None:intergenic 15.0%
!!! AAGATTGAAGATTTAAAATT+AGG - chr8.4:70295019-70295038 MS.gene82030:CDS 15.0%
!!! AGATTGAAGATTTAAAATTA+GGG - chr8.4:70295020-70295039 MS.gene82030:CDS 15.0%
! ATAAGATTCTTACTACAACT+AGG + chr8.4:70295142-70295161 None:intergenic 25.0%
! ATCTTAAAGCATAGAATCTA+TGG + chr8.4:70294919-70294938 None:intergenic 25.0%
! GACCGTTAATAAAGTTATAA+TGG - chr8.4:70295300-70295319 MS.gene82030:CDS 25.0%
!! TTTAAAATTAGGGCAACAAA+AGG - chr8.4:70295030-70295049 MS.gene82030:CDS 25.0%
!!! CAATTTTTTTTGCTTCTCAT+GGG + chr8.4:70294684-70294703 None:intergenic 25.0%
AATGAGTGTCTTGAAAGATT+TGG - chr8.4:70295249-70295268 MS.gene82030:CDS 30.0%
GAGATTGATGTGAAGATTAT+AGG - chr8.4:70295169-70295188 MS.gene82030:CDS 30.0%
TCTTCATTTCTCATCTCATT+AGG + chr8.4:70295349-70295368 None:intergenic 30.0%
!! ATTCTATGCTTTAAGATCTG+TGG - chr8.4:70294922-70294941 MS.gene82030:CDS 30.0%
!! TACTACAACTAGGTTTGTTT+TGG + chr8.4:70295132-70295151 None:intergenic 30.0%
!! TTATCCATCTTTGAAACGTT+TGG + chr8.4:70294950-70294969 None:intergenic 30.0%
!!! GCAATTTTTTTTGCTTCTCA+TGG + chr8.4:70294685-70294704 None:intergenic 30.0%
!!! GTTGTTTTTGTTGCTAATAG+TGG + chr8.4:70294773-70294792 None:intergenic 30.0%
AAAAGAGAACCGAAGAAAAG+AGG - chr8.4:70294818-70294837 MS.gene82030:CDS 35.0%
GAAATGAAGAGAGCCTAATT+AGG - chr8.4:70295359-70295378 MS.gene82030:CDS 35.0%
GATTATAGGACATGATGCTA+TGG - chr8.4:70295183-70295202 MS.gene82030:CDS 35.0%
TGAGAATTGCAGACCTAATT+AGG + chr8.4:70295375-70295394 None:intergenic 35.0%
TTTATCTCTCCTTTGTCTCT+CGG + chr8.4:70294891-70294910 None:intergenic 35.0%
! CTTGAAAGATTTGGAGTTTC+AGG - chr8.4:70295258-70295277 MS.gene82030:CDS 35.0%
! GGTTTTCTTCCTCTTTTCTT+CGG + chr8.4:70294830-70294849 None:intergenic 35.0%
! TTCTTTGTTATCAGATGCAG+TGG - chr8.4:70294973-70294992 MS.gene82030:CDS 35.0%
!!! TTTTTTTTGCTTCTCATGGG+TGG + chr8.4:70294681-70294700 None:intergenic 35.0%
ACACTCATTAATCGTGCACT+AGG + chr8.4:70295238-70295257 None:intergenic 40.0%
CACTCATTAATCGTGCACTA+GGG + chr8.4:70295237-70295256 None:intergenic 40.0%
GGTTCCAAACGTTTCAAAGA+TGG - chr8.4:70294943-70294962 MS.gene82030:CDS 40.0%
TCAAACACCGATGAACCATG+TGG - chr8.4:70294862-70294881 MS.gene82030:CDS 45.0%
TGCAACGACAACTTCAATCG+TGG - chr8.4:70295102-70295121 MS.gene82030:CDS 45.0%
! GGTGTTTGAGTTCGTGTCAA+CGG + chr8.4:70294851-70294870 None:intergenic 45.0%
ATGTGGAAGCCGAGAGACAA+AGG - chr8.4:70294879-70294898 MS.gene82030:CDS 50.0%
CTTCTCATGGGTGGCTTCTT+TGG + chr8.4:70294672-70294691 None:intergenic 50.0%
TCGGCTTCCACATGGTTCAT+CGG + chr8.4:70294872-70294891 None:intergenic 50.0%
TTTGTCTCTCGGCTTCCACA+TGG + chr8.4:70294880-70294899 None:intergenic 50.0%
ATGGGTGGCTTCTTTGGCGC+TGG + chr8.4:70294666-70294685 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 70294644 70295405 70294644 ID=MS.gene82030
chr8.4 mRNA 70294644 70295405 70294644 ID=MS.gene82030.t1;Parent=MS.gene82030
chr8.4 exon 70294644 70295405 70294644 ID=MS.gene82030.t1.exon1;Parent=MS.gene82030.t1
chr8.4 CDS 70294644 70295405 70294644 ID=cds.MS.gene82030.t1;Parent=MS.gene82030.t1
Gene Sequence

>MS.gene82030

ATGGGTGTCGTCTCTGGCGCCAGCGCCAAAGAAGCCACCCATGAGAAGCAAAAAAAATTGCAAGAAAAACATAAAAATGTTTCATCATGTTCTTTAGATTCTGAAGATTCTGATTCAGAATATTGTCCACTATTAGCAACAAAAACAACAACGACAAAGAACGATTCTTTTGAGAAAAGAGAACCGAAGAAAAGAGGAAGAAAACCGTTGACACGAACTCAAACACCGATGAACCATGTGGAAGCCGAGAGACAAAGGAGAGATAAACTAAACCATAGATTCTATGCTTTAAGATCTGTGGTTCCAAACGTTTCAAAGATGGATAAAGCTTCTTTGTTATCAGATGCAGTGGATTACATCAATGAGTTAAAAGCAAAGATTGAAGATTTAAAATTAGGGCAACAAAAGGAATCGAAAAAGTTGATGATGAAAACGATGAAAATTGTCGATAATAACAGTGCAACGACAACTTCAATCGTGGTAGACCAAAACAAACCTAGTTGTAGTAAGAATCTTATTGCTTTAGAGATTGATGTGAAGATTATAGGACATGATGCTATGGTGAGAGTTCAAAGTTTGAATGTTAATCACCCTAGTGCACGATTAATGAGTGTCTTGAAAGATTTGGAGTTTCAGGTACATCATGCTAGTATATCGACCGTTAATAAAGTTATAATGGTTCAAGATGTTATTGTTAAAATTCCTAATGAGATGAGAAATGAAGAGAGCCTAATTAGGTCTGCAATTCTCATGAAATTATGA

Protein sequence

>MS.gene82030.t1

MGVVSGASAKEATHEKQKKLQEKHKNVSSCSLDSEDSDSEYCPLLATKTTTTKNDSFEKREPKKRGRKPLTRTQTPMNHVEAERQRRDKLNHRFYALRSVVPNVSKMDKASLLSDAVDYINELKAKIEDLKLGQQKESKKLMMKTMKIVDNNSATTTSIVVDQNKPSCSKNLIALEIDVKIIGHDAMVRVQSLNVNHPSARLMSVLKDLEFQVHHASISTVNKVIMVQDVIVKIPNEMRNEESLIRSAILMKL